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spatial-temporal distribution of aedes (stegomyia) aegypti and locations of recycling units in southeastern brazil.dengue is an arbovirus disease that threatens approximately 200 million people annually worldwide. aedes (stegomyia) aegypti (linnaeus, 1762) is anthropophilic mosquito, extremely well adapted to the urban environment and utilizes varied habitats for egg-laying and development. this study analysed the distribution of mosquito larvae and eggs in urban area of santa bárbara d'oeste, são paulo, brazil. the spatial correlation between locations in which people store recyclable materials and the dist ...201931727179
dengue virus reduces agpat1 expression to alter phospholipids and enhance infection in aedes aegypti.more than half of the world population is at risk of dengue virus (denv) infection because of the global distribution of its mosquito vectors. denv is an envelope virus that relies on host lipid membranes for its life-cycle. here, we characterized how denv hijacks the mosquito lipidome to identify targets for novel transmission-blocking interventions. to describe metabolic changes throughout the mosquito denv cycle, we deployed a liquid chromatography-high resolution mass spectrometry (lc-hrms) ...201931815960
genetic diversity of laboratory strains and implications for research: the case of aedes aegypti.the yellow fever mosquito (aedes aegypti), is the primary vector of dengue, zika, and chikungunya fever, among other arboviral diseases. it is also a popular laboratory model in vector biology due to its ease of rearing and manipulation in the lab. established laboratory strains have been used worldwide in thousands of studies for decades. laboratory evolution of reference strains and contamination among strains are potential severe problems that could dramatically change experimental outcomes a ...201931815934
glopid-r report on chikungunya, o'nyong-nyong and mayaro virus, part 5: entomological aspects.the glopid-r (global research collaboration for infectious disease preparedness) chikungunya (chikv), o'nyong-nyong (onnv) and mayaro virus (mayv) working group has been established to investigate natural history, epidemiology and clinical aspects of infection by these viruses. here, we present a report dedicated to entomological aspects of chikv, onnv and mayv. recent global expansion of chikungunya virus has been possible because chikv established a transmission cycle in urban settings using a ...201931812638
cryo-electron microscopy reveals that sperm modification coincides with female fertility in the mosquito aedes aegypti.manipulating mosquito reproduction is a promising approach to reducing mosquito populations and the burden of diseases they carry. a thorough understanding of reproductive processes is necessary to develop such strategies, but little is known about how sperm are processed and prepared for fertilization within female mosquitoes. by employing cryo-electron microscopy for the first time to study sperm of the mosquito aedes aegypti, we reveal that sperm shed their entire outer coat, the glycocalyx, ...201931811199
assessment of aedes aegypti (diptera: culicidae) males flight ability for sit application: effect of device design, duration of test, and male age.the sterile insect technique (sit) is a pest control method where large numbers of sterile males are released to induce sterility in wild populations. since a successful sit application depends on the released sterile males being competitive with wild males, standard quality control tests are a necessary component of any sit program. flight ability (ability to fly out from a device) is a reliable indicator of insect quality. based on previous studies, we developed four new tubular devices constr ...201931808821
malaria and dengue mosquito vectors from lao pdr show a lack of the rdl mutant allele responsible for cyclodiene insecticide resistance.the gamma-aminobutyric acid (gaba) receptor, rdl, plays important roles in neuronal signaling and is the target of highly effective insecticides. a mutation in rdl, commonly a296s, underlies resistance to several insecticides such as cyclodienes. even though the use of cyclodienes has been banned, the occurrence of mutations substituting a296 is notably high in mosquitoes from several countries. here, we report a survey investigating the prevalence of the rdl mutant allele in mosquitoes from lao ...201931807752
the consequences of brugia malayi infection on the flight and energy resources of aedes aegypti mosquitoes.evidence from experimental infection studies has shown that infected mosquitoes exhibit altered host-seeking behaviours, with suppression and activation of behaviours dependent on the parasite's development stage. the mechanisms are poorly characterised; however, infections can impact mosquito energy reserves, thereby influencing key life-history traits and behaviours. in addition, filarial infection is likely detrimental to flight due to damage caused by developing worms. this study aimed to ev ...201931804546
finding the sweet spot: what levels of larval mortality lead to compensation or overcompensation in adult production?extrinsic mortality impinging on negatively density-dependent populations can result in no change in the number of survivors (compensation) or an increase (overcompensation) by releasing the population from density-dependent effects on survivorship. the relationship between the level of extrinsic mortality (i.e., percentage of mortality) and the level and likelihood of overcompensation is theoretically important, but rarely investigated. we tested the hypothesis that overcompensation occurs belo ...201931803516
diterpenes and their derivatives as promising agents against dengue virus and dengue vectors: a literature-based review.dengue, a mosquito-borne viral disease, causes about 100 million cases of infection annually. it is a major public concern, and if left untreated or improperly diagnosed, may cause serious health problems or even death. historically, dengue has not considered priorities for pharmaceutical companies made the available treatment options. therefore, medicinal scientists are revealing new insights and enabling novel interventions and approaches to dengue prevention and control. diterpenes, a class o ...201931802573
[river boats as a means of expansion of aedes aegypti towards border zones of the peruvian amazon].to assess the entomological risk of aedes aegypti in boats traversing border river routes in loreto.201931800930
investigating male aedes aegypti (diptera: culicidae) attraction to different oviposition containers using various configurations of the sound gravid aedes trap.aedes aegypti (linnaeus), the primary vectors of the arboviruses dengue virus and zika virus, continue to expand their global distributions. in efforts to better control such species, several mosquito control programs are investigating the efficacy of rearing and releasing millions of altered male aedes throughout landscapes to reduce populations and disease transmission risk. unfortunately, little is known about ae. aegypti, especially male, dispersal behaviors within urban habitats. we deploye ...201931799614
drug repurposing of bromodomain inhibitors as potential novel therapeutic leads for lymphatic filariasis guided by multispecies transcriptomics.to better understand the transcriptomic interplay of organisms associated with lymphatic filariasis, we conducted multispecies transcriptome sequencing (rna-seq) on the filarial nematode brugia malayi, its wolbachia endosymbiont wbm, and its laboratory vector aedes aegypti across the entire b. malayi life cycle. in wbm, transcription of the noncoding 6s rna suggests that it may be a regulator of bacterial cell growth, as its transcript levels correlate with bacterial replication rates. for a. ae ...201931796568
cdte-gsh as luminescent biomarker for labeling the larvicidal action of wsmol lectin in aedes aegypti larvae.mosquito-borne arboviruses compromise human health worldwide. due to resistance to chemical insecticides, natural compounds have been studied to combat mosquitoes. previous works have demonstrated a larvicidal activity of the water-soluble moringa oleifera lectin (wsmol) against aedes aegypti, suggesting a mechanism of action based on the interaction between lectin and chitin present in the larvae's peritrophic matrix. in this work, it was investigated the wsmol activity against aedes aegypti la ...201931796242
genome sequences of chikungunya virus isolates from an outbreak in southwest bangkok in 2018.an outbreak of chikungunya virus (chikv) infection occurred in southwest bangkok during the 2018 rainy season. the envelope glycoprotein e1 gene sequence of the infecting strain belonged to an east/central/south african lineage with alanine at residue 226. mutations in the predicted e1 (k211e) and e2 (v264a) proteins of chikv were identified in chikv-infected patients and in an aedes aegypti mosquito. analysis of the complete genome sequences showed marked differences from the strains causing pr ...201931834526
distribution of insecticide resistance and mechanisms involved in the arbovirus vector aedes aegypti in laos and implication for vector control.the yellow fever mosquito aedes aegypti is the major vector of dengue, yellow fever, zika, and chikungunya viruses. worldwide vector control is largely based on insecticide treatments but, unfortunately, vector control programs are facing operational challenges due to mosquitoes becoming resistant to commonly used insecticides. in southeast asia, resistance of ae. aegypti to chemical insecticides has been documented in several countries but no data regarding insecticide resistance has been repor ...201931830027
effects of larval diets and temperature regimes on life history traits, energy reserves and temperature tolerance of male aedes aegypti (diptera: culicidae): optimizing rearing techniques for the sterile insect programmes.producing high quality sterile males is vital in aedes aegypti rear-and-release birth control strategies. larval diets, rearing temperatures, and their interactions determine the accumulation rates of essential nutrients in larvae, but these factors have been understudied in relation to mass-rearing techniques for producing eminent males.201931823817
social capital is associated with lower mosquito vector indices: secondary analysis from a cluster randomised controlled trial of community mobilisation for dengue prevention in mexico.control of the aedes aegypti mosquito is central to reducing the risk of dengue, zika, chikungunya, and yellow fever. randomised controlled trials, including the camino verde trial in mexico and nicaragua, demonstrate the convincing impact of community mobilisation interventions on vector indices. these interventions might work through building social capital but little is known about the relationship between social capital and vector indices.201931823786
zika virus: a possible emerging threat for bangladesh!zika virus, a member of flaviviridae is the etiology of zika or zika fever or zika virus (zikv) disease characterized by mild symptoms similar to very mild form of dengue or chikungunya. the virus transmits through aedes mosquitoes, particularly by aedes aegypti. the most dangerous effect of zikv infection is the ability of the virus to cause microcephaly and congenital malformation to the newborn baby if the mother is infected. the neurological disorders including guillain-barré syndrome might ...201931819889
successive blood meals enhance virus dissemination within mosquitoes and increase transmission potential.the recent zika virus (zikv) and chikungunya virus epidemics highlight the explosive nature of arthropod-borne viruses (arboviruses) transmitted by aedes spp. mosquitoes1,2. vector competence and the extrinsic incubation period (eip) are two key entomological parameters used to assess the public health risk posed by arboviruses3. these are typically measured empirically by offering mosquitoes an infectious blood meal and temporally sampling mosquitoes to determine the infection and transmission ...201931819213
natural infection of aedes aegypti by chikungunya and dengue type 2 virus in a transition area of north-northeast brazil.dengue fever, chikungunya, and zika are diseases caused by viruses transmitted by aedes aegypti and aedes albopictus. in brazil, the number of human infections is high, but few studies are performed in mosquito vectors. this study aimed to investigate the presence of zika, dengue and chikungunya viruses in ae. aegypti and ae. albopictus from the municipalities of alto alegre, caxias, codó, and são mateus do maranhão, located in the state of maranhão, northeast brazil. the mosquitoes were collect ...201931817553
zika virus disease.the zika virus is an arbovirus belonging to the virus family flaviviridae. the virus was isolated in 1947 from a rhesus monkey in the zika forest of uganda. the virus causes sporadic mild human infections in africa and later in asia. however, by 2007 a major shift in its infection pattern was noticed and thousands of human infections were reported in the state of yap and federated states of micronesia. in the last 3 years, major outbreaks have continued to occur and the virus has spread to sever ...201930787745
[coinfection of dengue and chikungunya viruses. narrative review].dengue and chikungunya viruses are transmitted by the female aedes aegypti and aedes albopictus, which are widely distributed in tropical and subtropical areas, facilitating coinfection.201931095186
high frequencies of f1534c and v1016i kdr mutations and association with pyrethroid resistance in aedes aegypti from somgandé (ouagadougou), burkina faso.resistance to pyrethroid insecticides involving kdr mutations is widespread in aedes aegypti (l.) (diptera: culicidae) and potentially could impact control efforts in endemic countries. dengue cases had been sporadic in burkina faso for over a decade prior to the 2016-2017 outbreak that resulted in 15,074 suspected cases and 36 deaths, mainly in ouagadougou. these outbreaks highlighted the lack of information on numerous aspects of the biology, behaviour and insecticide status of local dengue ve ...201930787670
experimental study on the action of larvicides in aedes aegypti populations collected in the brazilian municipality of itabuna, bahia, under simulated field conditions.to evaluate, under simulated field conditions, the efficacy of pyriproxyfen (juvenile hormone), novaluron (chitin inhibitor) and spinosad (biolarvicide) in controlling aedes aegypti.201930785572
entomological and virological surveillance for dengue virus in churches in merida, mexico.this study was designed to assess whether churches in endemic dengue districts in merida, mexico provide suitable breeding habitats for mosquitoes and are potential sites for dengue virus (denv) transmission. churches were inspected for immature and adult mosquitoes once every week from november 2015 to october 2016. a total of 10,997 immatures of five species were collected. the most abundant species were aedes aegypti (6,051) and culex quinquefasciatus (3,018). the most common source of immatu ...201930785563
origin and expansion of the mosquito aedes aegypti in madeira island (portugal).historically known as the yellow fever mosquito, aedes aegypti invaded madeira island in 2005 and was the vector of the island's first dengue outbreak in 2012. we have studied genetic variation at 16 microsatellites and two mitochondrial dna genes in temporal samples of madeira island, in order to assess the origin of the invasion and the population structure of this mosquito vector. our results indicated at least two independent colonization events occurred on the island, both having a south am ...201930783149
optimization of irradiation dose to aedes aegypti and ae. albopictus in a sterile insect technique program.the sterile insect technique (sit) may offer a means to control the transmission of mosquito borne diseases. sit involves the release of male insects that have been sterilized by exposure to ionizing radiation. we determined the effects of different doses of radiation on the survival and reproductive capacity of local strains of aedes aegypti and ae. albopictus in southern mexico. the survival of irradiated pupae was invariably greater than 90% and did not differ significantly in either sex for ...201930779779
larvicidal and ovicidal activities of artocarpus blancoi extracts against aedes aegypti.dengue control may be done by targeting its vector. in this study, we used artocarpus blancoi (elm.) merr. (moraceae) leaves, an endemic hematophagous insect repellent as a larvicide and ovicide.201930776315
natural vertical infection by dengue virus serotype 4, zika virus and mayaro virus in aedes (stegomyia) aegypti and aedes (stegomyia) albopictus.vertical transmission to progeny ensures the maintenance of arboviruses in their natural vectors. this mechanism is largely reported for dengue virus (denv) and yellow fever virus (yfv). few studies have addressed this mechanism for zika virus (zikv), mayaro virus (mayv) and other arboviruses. the present study investigated the natural infection rate by arboviruses in 4490 aedes (stegomyia) aegypti and 296 aedes (stegomyia) albopictus (diptera: culicidae) reared from eggs collected with ovitraps ...201930776139
interventions for the control of aedes aegypti in latin america and the caribbean: systematic review and meta-analysis.to determine the effectiveness and degree of implementation of interventions for the control of aedes aegypti in latin america and the caribbean (lac) as reported in scientific literature.201930771267
juvenile hormone controls ovarian development in female anopheles albimanus mosquitoes.anophelinae mosquitoes are vectors of human malaria, a disease that infects hundreds of millions of people and causes almost 600,000 fatalities annually. despite their medical importance, laboratory studies on key aspects of anophelinae reproductive biology have been limited, and in particular, relatively little is known about the role of juvenile hormone (jh) in the control of female reproduction. the study presented here attempts to fill a gap of knowledge in our understanding of the jh contro ...201930765796
female resistance and harmonic convergence influence male mating success in aedes aegypti.despite the importance of mosquito mating biology to reproductive control strategies, a mechanistic understanding of individual mating interactions is currently lacking. using synchronised high-speed video and audio recordings, we quantified behavioural and acoustic features of mating attempts between tethered female and free-flying male aedes aegypti. in most couplings, males were actively displaced by female kicks in the early phases of the interaction, while flight cessation prior to adoption ...201930765779
surveillance of aedes aegypti indoors and outdoors using autocidal gravid ovitraps in south texas during local transmission of zika virus, 2016 to 2018.the yellow fever mosquito, aedes aegypti, has facilitated the re-emergence of dengue virus (denv) and emergence of chikungunya virus (chikv) and zika virus (zikv) in the americas and the caribbean. the recent transmission of these arboviruses in the continental united states has been limited, to date, to south florida and south texas despite ae. aegypti occurring over a much larger geographical region within the country. the main goal of our study was to provide the first long term longitudinal ...201930763563
potential for sylvatic and urban aedes mosquitoes from senegal to transmit the new emerging dengue serotypes 1, 3 and 4 in west africa.dengue fever (den) is the most common arboviral disease in the world and dengue virus (denv) causes 390 million annual infections around the world, of which 240 million are inapparent and 96 million are symptomatic. during the past decade a changing epidemiological pattern has been observed in africa, with den outbreaks reported in all regions. in senegal, all denv serotypes have been reported. these important changes in the epidemiological profile of den are occurring in a context where there i ...201930759080
exome-wide association of deltamethrin resistance in aedes aegypti from mexico.aedes aegypti is the major vector of a number of arboviruses that cause disease in humans. without vaccines or pharmaceuticals, pyrethroid insecticides remain the major tool for public health protection. pyrethroid resistance is now widespread. replacement substitutions in the voltage-gated sodium channel (vgsc) that reduce the stability of pyrethroid binding account for most of the resistance, but metabolic mechanisms also inactivate pyrethroids. high-throughput sequencing and the a. aegypti l5 ...201930758862
risk of dengue transmission in cocody (abidjan, ivory coast).in recent years, an upsurge of vector-borne diseases has been reported in several parts of the world. among these is dengue fever, the first arbovirus transmitted by mosquitoes of the genus aedes. after the detection of the dengue virus serological types (type 1, 2, and 3) in the health district of cocody-bingerville in ivory coast, entomological investigations were carried out in the city of cocody (host of most cases) to evaluate the risk of transmission of the disease in view of an effective ...201930755798
[effect of attraction of transfluthrin and linalool on the mosquito vector aedes aegypti]. 201930753763
citywide control of aedes aegypti (diptera: culicidae) during the 2016 zika epidemic by integrating community awareness, education, source reduction, larvicides, and mass mosquito trapping.this investigation was initiated to control aedes aegypti and zika virus transmission in caguas city, puerto rico, during the 2016 epidemic using integrated vector management (ivm), which included community awareness and education, source reduction, larviciding, and mass-trapping with autocidal gravid ovitraps (ago). the epidemic peaked in august to october 2016 and waned after april 2017. there was a preintervention period in october/november 2016 and ivm lasted until august 2017. the area unde ...201930753539
quantitative evaluation of the behavioral response to attractant and repellent compounds in anopheles pseudopunctipennis and aedes aegypti (diptera: culicidae) larvae.the mosquito anopheles pseudopunctipennis (theobald) is the principal vector for malaria in latin-america. aedes aegypti (l.) (diptera: culicidae) is the key vector of four important arboviral diseases: dengue, yellow fever, zika, and chikungunya. controlling larval stages to reduce the production of new adult mosquitoes is part of the integrated vector management strategies. however, there are few studies about the olfactory behavior on immature stages of mosquitoes, especially in an. pseudopun ...201930753516
vector competence of biting midges and mosquitoes for shuni virus.shuni virus (shuv) is an orthobunyavirus that belongs to the simbu serogroup. shuv was isolated from diverse species of domesticated animals and wildlife, and is associated with neurological disease, abortions, and congenital malformations. recently, shuv caused outbreaks among ruminants in israel, representing the first incursions outside the african continent. the isolation of shuv from a febrile child in nigeria and seroprevalence among veterinarians in south africa suggests that the virus ma ...201930753187
feasibility of feeding aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in iquitos, peru.transmission of dengue virus (denv) from humans to mosquitoes represents a critical component of dengue epidemiology. examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of denv by mosquitoes from humans. in this study, we assessed artificial and natural blood feeding methods based on rates of denv infection and dissemination within mosquitoes for use in a field-based epidemiological cohort study in iquitos, peru.201930753180
bacteria: a novel source for potent mosquito feeding-deterrents.antibiotic and insecticidal bioactivities of the extracellular secondary metabolites produced by entomopathogenic bacteria belonging to genus xenorhabdus have been identified; however, their novel applications such as mosquito feeding-deterrence have not been reported. here, we show that a mixture of compounds isolated from xenorhabdus budapestensis in vitro cultures exhibits potent feeding-deterrent activity against three deadly mosquito vectors: aedes aegypti, anopheles gambiae, and culex pipi ...201930746455
the long and short of hearing in the mosquito aedes aegypti.mating behavior in aedes aegypti mosquitoes occurs mid-air and involves the exchange of auditory signals at close range (millimeters to centimeters) [1-6]. it is widely assumed that this intimate signaling distance reflects short-range auditory sensitivity of their antennal hearing organs to faint flight tones [7, 8]. to the contrary, we show here that male mosquitoes can hear the female's flight tone at surprisingly long distances-from several meters to up to 10 m-and that unrestrained, resting ...201930744970
detection of rna-dependent rna polymerase of hubei reo-like virus 7 by next-generation sequencing in aedes aegypti and culex quinquefasciatus mosquitoes from brazil.advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. hubei reo-like virus 7 (hrlv 7) was recently detected by the virome analysis of fecal samples from migratory birds in australia. we now report the detection of rna-dependent rna polymerase sequences of hrlv 7 in pools of aedes aegypti and culex quinquefasciatus mosquitoes species from the brazilian amazon forest. phylogenetic ...201930744159
modeling the present and future distribution of arbovirus vectors aedes aegypti and aedes albopictus under climate change scenarios in mainland china.aedes aegypti and aedes albopictus are two important mosquito species which transmit various infectious arbovirus diseases represented mainly by dengue fever. these two species of mosquito have a wide range of distribution and strong transfer capacity. with ongoing global climate change, we are facing an increasing public health threat from the rapid spread of vectors in wider geographical areas. based on observed occurrence records of ae. aegypti and ae. albopictus and high-resolution environme ...201930743113
acceptability of aedes aegypti blood feeding on dengue virus-infected human volunteers for vector competence studies in iquitos, peru.as part of a study to investigate drivers of dengue virus (denv) transmission dynamics, this qualitative study explored whether denv-infected residents of iquitos, peru, considered it acceptable (1) to participate in direct mosquito feeding experiments (lab-reared aedes aegypti mosquitoes fed directly on human volunteers) and (2) to provide blood meals indirectly (ae. aegypti fed on blood drawn from participants by venipuncture). twelve focus group discussions (fgds; 94 participants: 82 females ...201930742621
mathematical modeling of dengue epidemic: control methods and vaccination strategies.dengue is, in terms of death and economic cost, one of the most important infectious diseases in the world. so, its mathematical modeling can be a valuable tool to help us to understand the dynamics of the disease and to infer about its spreading by the proposition of control methods. in this paper, control strategies, which aim to eliminate the aedes aegypti mosquito, as well as proposals for the vaccination campaign are evaluated. in our mathematical model, the mechanical control is accomplish ...201930740641
small-molecule agonists of ae. aegypti neuropeptide y receptor block mosquito biting.female aedes aegypti mosquitoes bite humans to obtain blood to develop their eggs. remarkably, their strong attraction to humans is suppressed for days after the blood meal by an unknown mechanism. we investigated a role for neuropeptide y (npy)-related signaling in long-term behavioral suppression and discovered that drugs targeting human npy receptors modulate mosquito host-seeking. in a screen of all 49 predicted ae. aegypti peptide receptors, we identified npy-like receptor 7 (npylr7) as the ...201930735632
the perfect appetizer: a pharmacological strategy for a non-biting mosquito.new possibilities for vector-borne disease control are revealed by duvall et al. (2019), who link host-seeking behavioral modulation in aedes aegypti to neuropeptide y (npy)-like receptor 7. small-molecule screening yields agonist compounds able to activate npylr7 and suppress attraction to hosts.201930735629
loop-mediated isothermal amplification (lamp) for the identification of invasive aedes mosquito species.invasive aedes mosquito species (diptera: culicidae) are of public health concern in europe because they are either recognized or potential vectors of pathogens. loop-mediated isothermal amplification (lamp) is a rapid and simple method for amplifying dna with high specificity and efficiency, with the technique having potential for application in the field, including in high-throughput format. specific lamp assays based on rdna internal transcribed spacers 1 or 2 sequences, considering intraspec ...201930734975
genome sequences of chikungunya virus isolates circulating in midwestern brazil.chikungunya virus (chikv) is a reemerging arbovirus of the family togaviridae that causes chikv fever, a disease that can extend from weeks to years depending on whether clinical signs of arthralgia persist. chikv is mainly transmitted by aedes aegypti mosquitoes and possibly reached the americas in 2013, causing an outbreak in brazil in 2015. so far, two evolutionary lineages of chikv have been reported in brazil: the asian and the east-central-south african (ecsa) lineages. in this study, six ...201930729309
repellency assessment of nepeta cataria essential oils and isolated nepetalactones on aedes aegypti.there is an increased need for improved and affordable insect repellents to reduce transmission of rapidly spreading diseases with high mortality rates. natural products are often used when deet cannot be afforded or accessed and when consumers choose not to use a synthetic repellent. the essential oils from two newly bred nepeta cataria (catnip) plants representing two different chemotypes and their respective isolated nepetalactone isomers were evaluated as mosquito repellents against aedes ae ...201930728370
engineered resistance to zika virus in transgenic aedes aegypti expressing a polycistronic cluster of synthetic small rnas.recent zika virus (zikv) outbreaks have highlighted the necessity for development of novel vector control strategies to combat arboviral transmission, including genetic versions of the sterile insect technique, artificial infection with wolbachia to reduce population size and/or vectoring competency, and gene drive-based methods. here, we describe the development of mosquitoes synthetically engineered to impede vector competence to zikv. we demonstrate that a polycistronic cluster of engineered ...201930723148
genotoxic and mutagenic assessment of spinosad using bioassays with tradescantia pallida and drosophila melanogaster.spinosad (spn) is a naturally-occurring insecticide obtained from the fermentation process of the actinomycete saccharopolyspora spinosa. owing to the larvicidal action, the compound has been used in the control of aedes aegypti. as a new insecticide commercially available in the market, few data are reported on genotoxic effects in non-target organisms. the objective of the present study was to evaluate the mutagenic effect of spn through the micronucleus test in tradescantia pallida (trad-mcn) ...201930721808
assessment of acute toxicity and cytotoxicity of fluorescent markers produced by cardanol and glycerol, which are industrial waste, to different biological models.the amphyphylic triazoanilines recently synthesized 1-(4-(3-aminophenyl)-1h-1,2,3- triazole-1-yl)-3-(3-pentadecylphenoxy)propan-2-ol (1) and 1-(4-(4-aminophenyl)-1h- 1,2,3-triazole-1-yl)-3-(3-pentadecylphenoxy)propan-2-ol (2), synthesized from cardanol and glycerol, have photophysical properties which allow their use in the development of fluorescent biomarkers with applicability in the biodiesel quality control. based on this, the present research evaluated the toxic effects of both compounds i ...201930719661
evaluation of enhanced oviposition attractant formulations against aedes and culex vector mosquitoes in urban and semi-urban areas.surveillance is not only an important tool to assess the population dynamics of vector mosquitoes, but it can also be used to control vector-borne diseases. mosquito vectors that belong to several genera such as anopheles, aedes, and culex play a crucial role in the transmission of malaria, dengue, chikungunya, zika, and elephantiasis diseases worldwide. we tested the efficacy of two commercial-grade oviposition attractant formulations that were developed for the container-inhabiting aedes aegyp ...201930719534
homologs of human dengue-resistance genes, fkbp1b and atcay, confer antiviral resistance in aedes aegypti mosquitoes.dengue virus (denv) is transmitted by mosquitoes and is a major public health concern. the study of innate mosquito defense mechanisms against denv have revealed crucial roles for the toll, imd, jak-stat, and rnai pathways in mediating denv in the mosquito. often overlooked in such studies is the role of intrinsic cellular defense mechanisms that we hypothesize to work in concert with the classical immune pathways to affect organismal defense. our understanding of the molecular interaction of de ...201930717390
attraction of female aedes aegypti (l.) to aphid honeydew.plant sugar is an essential dietary constituent for mosquitoes, and hemipteran honeydew is one of the many forms of plant sugar that is important to mosquitoes. many insects rely on volatile honeydew semiochemicals to locate aphids or honeydew itself. mosquitoes exploit volatile semiochemicals to locate sources of plant sugar but their attraction to honeydew has not previously been investigated. here, we report the attraction of female yellow fever mosquitoes, aedes aegypti, to honeydew odorants ...201930717169
analysis of salivary glands and saliva from aedes albopictus and aedes aegypti infected with chikungunya viruses.chikungunya virus (chikv) is a medically important mosquito-borne virus transmitted to humans by infected aedes (stegomyia) species. in 2013⁻2014, ae. aegypti transmitted chikv to humans in the caribbean and in 2005⁻2006, ae. albopictus transmitted chikv on la réunion island (indian ocean basin). chikv lr2006 opy1 from the la réunion epidemic was associated with a mutation (e1:a226v) in the viral e1 glycoprotein that enhanced chikv transmission by ae. albopictus. chikv r99659 from the caribbean ...201930717086
water-induced strong protection against acute exposure to low subzero temperature of adult aedes albopictus.as an important vector of dengue and zika, aedes albopictus has been the fastest spreading invasive mosquitoes in the world over the last 3-4 decades. cold tolerance is important for survival and expansion of insects. ae. albopictus adults are generally considered to be cold-intolerant that cannot survive at subzero temperature. however, we found that ae. albopictus could survive for several hours' exposure to -9 to -19 oc so long as it was exposed with water. median lethal time (lt50) of ae. al ...201930716071
pyrethroids use: threats on metabolic-mediated resistance mechanisms in the primary dengue vector aedes aegypti (diptera: culicidae).the emergence of pyrethroid resistance in aedes aegypti (l.) has limited the success of vector control. early detection of resistance could assist authorities in deciding well-suited control strategies to minimize operational failures of ae. aegypti control. herein, biochemical analysis was performed to investigate the mechanisms involved in pyrethroid resistance in nine populations of indonesian ae. aegypti. enzymes of adult ae. aegypti such as esterases (ests), glutathione-s-transferases (gsts ...201930715464
transcriptional profile of aedes aegypti leucine-rich repeat proteins in response to zika and chikungunya viruses.aedes aegypti (l.) is the primary vector of chikungunya, dengue, yellow fever, and zika viruses. the leucine-rich repeats (lrr)-containing domain is evolutionarily conserved in many proteins associated with innate immunity in invertebrates and vertebrates, as well as plants. we focused on the aaelrim1 and aaeapl1 gene expressions in response to zika virus (zikv) and chikungunya virus (chikv) infection using a time course study, as well as the developmental expressions in the eggs, larvae, pupae, ...201930708982
glycosaminoglycan compositional analysis of relevant tissues in zika virus pathogenesis and in vitro evaluation of heparin as an antiviral against zika virus infection.zika virus (zikv) is an enveloped rna virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women. previously, we established that zikv-ep (envelope protein) binds to human placental chondroitin sulfate (cs), suggesting that cs may be a potential host cell surface receptor in zikv pathogenesis. in this study, we further characterized the gag disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in zikv pathoge ...201930698412
presence of the point mutations val1016gly in the voltage-gated sodium channel detected in a single mosquito from panama.in panama, arboviroses such as dengue fever, and more recently chikungunya fever and zika disease, are transmitted by aedes aegypti and aedes albopictus. their control is based on the elimination of breeding sites and fogging with pyrethroid insecticides. however, one of the significant issues derived from the prolonged use of pyrethroid insecticide is the development of resistance mechanisms, such as knockdown resistance or kdr. the objective of this study was to evaluate the presence of kdr mu ...201930691518
identification of potential inhibitors from pyriproxyfen with insecticidal activity by virtual screening.aedes aegypti is the main vector of dengue fever transmission, yellow fever, zika, and chikungunya in tropical and subtropical regions and it is considered to cause health risks to millions of people in the world. in this study, we search to obtain new molecules with insecticidal potential against ae. aegypti via virtual screening. pyriproxyfen was chosen as a template compound to search molecules in the database zinc_natural_stock (znst) with structural similarity using rocs (rapid overlay of c ...201930691028
factors affecting short-range host-seeking for the yellow fever mosquito (diptera: culicidae).understanding short-range cues (e.g., host odorants, heat, moisture) of host-seeking female aedes aegypti l. is very important for attempts to reduce mosquito bites, to complement current control strategies, and to develop potential spatial repellents. we investigated behavior under semi-field conditions utilizing a new portable uni-port taxis box with a caged chicken host. the combined influences of airflow regimes (0, 1.5, 3, 5, and 6 m/s), distance from host odor (10, 50, 100 cm), host-odor c ...201930689913
predation on eggs of aedes aegypti (diptera: culicidae): temporal dynamics and identification of potential predators during the winter season in a temperate region.in temperate regions, the populations of aedes aegypti (l.) remain in the egg stage during the cold season. the ability of these eggs to survive until the next favorable season is affected by several mortality factors, including the action of predators. in the present study, we analyzed the temporal dynamics of the loss of eggs and identified predators of ae. aegypti eggs along the unfavorable season in a temperate region of argentina. to this end, eggs were exposed in field conditions in pitfal ...201930689912
microbial exopolymer-capped selenium nanowires - towards new antibacterial, antibiofilm and arbovirus vector larvicides?arboviral diseases and microbial pathogens resistant to commercially available drugs are on the rise. herein, a facile microbial-based approach was developed to synthesize selenium nanowires (se nws) using microbial exopolymer (mep) extracted from the bacillus licheniformis (probiotic bacteria). mep-se nws were characterized using uv-visible, xrd, ftir, hr-tem, fe-sem and edx. an uv-visible peak was detected at 330 nm while xrd spectrum data pointed out the crystalline nature of mep-se nws. ftir ...201930685584
complete nmr chemical shift assignments of odorant binding protein 22 from the yellow fever mosquito, aedes aegypti, bound to arachidonic acid.aedes aegypti mosquitoes are the vector for transmission of dengue, zika and chikungunya viruses. these mosquitos feed exclusively on human hosts for a blood meal. previous studies have established that dengue virus infection of the mosquito results in increased expression of the odorant binding proteins 22 and 10 within the mosquito salivary gland and silencing of these genes dramatically reduces blood-feeding behaviors. odorant binding proteins are implicated in modulating the chemosensory per ...201930684234
toxicity and possible mechanisms of action of honokiol from magnolia denudata seeds against four mosquito species.this study was performed to determine the toxicity and possible mechanism of the larvicidal action of honokiol, extracted from magnolia denudata seeds, and its 10 related compounds against third-instar larvae of insecticide-susceptible culex pipiens pallens, aedes aegypti, and aedes albopictus and anopheles sinensis resistant to deltamethrin and temephos. honokiol (lc50, 6.13-7.37 mg/l) was highly effective against larvae of all of the four mosquito species, although the toxicity of the compound ...201930674912
chemical composition, aedes mosquito larvicidal activity, and repellent activity against triatoma rubrofasciata of severinia monophylla leaf essential oil.aedes aegypti and ae. albopictus are key vectors in the spread of arboviruses such as dengue, chikungunya, yellow fever, and zika. triatoma rubrofasciata is an "assassin bug" whose populations and association with humans have dramatically increased and may represent a serious health concern. control of insect vectors is a logical course of action to prevent the spread of these insect-borne infections. this work presents the leaf essential oil composition, mosquito larvicidal activities, and inse ...201930671730
rapid loss of resistance to satyrization in invasive mosquitoes and the effects of age on interspecific mating frequency.in several areas where aedes aegypti (l.) (diptera: culicidae) and aedes albopictus (skuse) (diptera: culicidae) have come in contact following successful invasions, ae. aegypti have been rapidly displaced by ae. albopictus. recent work has confirmed that mating interference, in the form of satyrization, is likely a driving factor in these competitive displacements. however, in sites of sympatry, ae. aegypti females evolve resistance to satyrization, and in the laboratory, satyrization-susceptib ...201930668807
florida aedes aegypti (diptera: culicidae) and aedes albopictus vector competency for zika virus.zika virus (zikv) first spread into brazil in 2013 and is now present throughout the americas. in 2016, florida witnessed the beginnings of local zikv transmission. no reports of local transmission have been reported for 2018; however, travel related cases continue to be reported. recurrence of local transmission in the united states is a major public health risk in florida where vectors aedes aegypti (diptera: culicidae, linnaeus) and aedes albopictus (diptera: culicidae, skuse) are abundant an ...201930668772
infection pattern of mayaro virus in aedes aegypti (diptera: culicidae) and transmission potential of the virus in mixed infections with chikungunya virus.mayaro virus (mayv; togaviridae; alphavirus) has drawn increasing attention as an arthropod-borne virus with potential to cause outbreaks among the human populations of the western hemisphere. in the tropical regions of central and south america, the virus exists in sylvatic cycles between mosquitoes and primate reservoirs such as marmosets. although forest-dwelling mosquitoes are regarded as important vectors for mayv, it has been shown previously that the virus can infect and potentially be tr ...201930668762
response of aedes aegypti and aedes albopictus (diptera: culicidae) survival, life history, and population growth to oak leaf and acorn detritus.aedes albopictus (skuse) (diptera: culicidae) and aedes aegypti (l.) (diptera: culicidae) are invasive container mosquitoes that are of potential medical importance in the southern united states. seeds (acorns) and leaves from oak trees can contribute seasonally to the detritus of larval container habitats. herein, we examined the effect of acorns and leaves from the southern live oak (quercus virginiana mill.), which has a concomitant range with these mosquitoes, on the population performance o ...201930668756
spermathecal filling in aedes aegypti and aedes albopictus: effects of female and male body sizes and species.the mosquitoes aedes aegypti (l.) (diptera: culicidae) and aedes albopictus (skuse) (diptera: culicidae) are ecologically similar species that have evolved independently in their native ranges, ae. aegypti in africa and ae. albopictus in asia. where their invasive ranges overlap, interspecific mating is facilitated by both species swarming to mate around bloodmeal hosts during daylight. here, we test hypotheses to account for variation in spermathecal filling in females of ae. aegypti and ae. al ...201930668724
arbovirus coinfection and co-transmission: a neglected public health concern?epidemiological synergy between outbreaks of viruses transmitted by aedes aegypti mosquitoes, such as chikungunya, dengue, and zika viruses, has resulted in coinfection of humans with multiple viruses. despite the potential impact on public health, we know only little about the occurrence and consequences of such coinfections. here, we review the impact of coinfection on clinical disease in humans, discuss the possibility for co-transmission from mosquito to human, and describe a role for modeli ...201930668574
entomological and sociobehavioral components of heartworm (dirofilaria immitis) infection in two florida communities with a high or low prevalence of dogs with heartworm infection.objective to assess knowledge, attitudes, and practices regarding heartworms (dirofilaria immitis) and mosquito vectors among residents (dog owners and non-dog owners) in 2 neighborhoods in florida and to perform entomological surveys of mosquito species in these neighborhoods and identify mosquito species infected with heartworm. design cross-sectional study. sample 2,572 mosquitoes and 96 residents of 2 northern florida communities. procedures a 32-item questionnaire was orally administered to ...201930668298
effect of meteorological factors on the seasonal prevalence of dengue vectors in upland hilly and lowland terai regions of nepal.the expansion of dengue vectors from lowland plains to the upland hilly regions of nepal suggests the likelihood of increased risk of dengue. our objective was to assess the effects of meteorological variables on vector indices and populations of dengue vectors in two different ecological regions of nepal. an entomological survey was conducted in kathmandu and lalitpur (upland) and chitwan (lowland) of nepal in three different seasons from july 2015 to may 2016. the effect of meteorological vari ...201930658693
aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity.the mosquito aedes aegypti is the main vector of dengue, chikungunya, zika, and yellow fever viruses, which are responsible for high human morbidity and mortality. the fight against these pathogens is mainly based on the control of the insect vector with the use of insecticides. among insecticides, spinosad bioinsecticide is efficient against a. aegypti larvae and may be an alternative for vector control. here, we investigate the sublethal effects of spinosad during midgut metamorphosis of a. ae ...201930654260
a role for the insulin receptor in the suppression of dengue virus and zika virus in wolbachia-infected mosquito cells.wolbachia-infected mosquitoes are refractory to super-infection with arthropod-borne pathogens, but the role of host cell signaling proteins in pathogen-blocking mechanisms remains to be elucidated. here, we use an antibody microarray approach to provide a comprehensive picture of the signaling response of aedes aegypti-derived cells to wolbachia. this approach identifies the host cell insulin receptor as being downregulated by the bacterium. furthermore, sirna-mediated knockdown and treatment w ...201930650347
extremely long viability of aedes aegypti (diptera: culicidae) eggs stored under normal room condition.the viability of aedes aegypti (linnaeus) eggs was determined by storing the eggs for long period of up to 1,889 d. the viability of eggs declined over time from 88.54 to 4.89% for the storage period of 1-1,883 d (5.15 yr). the percentage of collapsed eggs was inversely proportional to the hatching rate of eggs. in the first day of storage, the eggs remained healthy and intact, and except a few, the eggs were deformed and boat shaped with cleft due to inward shrinkage during long storage of 1,88 ...201930649399
cross-generational effects of heat stress on fitness and wolbachia density in aedes aegypti mosquitoes.aedes aegypti mosquitoes infected with wolbachia symbionts are now being released into the field to control the spread of pathogenic human arboviruses. wolbachia can spread throughout vector populations by inducing cytoplasmic incompatibility and can reduce disease transmission by interfering with virus replication. the success of this strategy depends on the effects of wolbachia on mosquito fitness and the stability of wolbachia infections across generations. wolbachia infections are vulnerable ...201930642130
application of convolutional neural networks for classification of adult mosquitoes in the field.dengue, chikungunya and zika are arboviruses transmitted by mosquitos of the genus aedes and have caused several outbreaks in world over the past ten years. morphological identification of mosquitos is currently restricted due to the small number of adequately trained professionals. we implemented a computational model based on a convolutional neural network (cnn) to extract features from mosquito images to identify adult mosquitoes from the species aedes aegypti, aedes albopictus and culex quin ...201930640961
genome-wide transcriptome profiling reveals genes associated with meiotic drive system of aedes aegypti.aedes aegypti is an important mosquito vector of several arboviruses, including dengue, yellow fever, zika, and chikungunya, which cause significant human morbidity and mortality globally. in certain populations of this mosquito, a native meiotic drive system causes abnormal spermatogenesis that results in highly male-biased progenies from some matings. although the basic genetics and cytogenetics of the drive mechanism were elucidated, very little is known on a transcriptome level about how the ...201930634571
aspergillus flavus (link) toxins reduces the fitness of dengue vector aedes aegypti (linn.) and their non-target toxicity against aquatic predator.mosquito that accountable for dispersal of dengue fever is aedes aegypti linn. and considered to be a chief vector for dengue especially in south asian countries. aspergillus flavus is considered to be wild growing green yellow colonies and synthesis highly regulating aflatoxins (b1, b2, g1 and g2) as a secondary metabolite. mycotoxins of a. flavus showed its efficacy against iii and iv instars of ae. aegypti with more than 90% mortality at the prominent dosage of 2 × 108 conidia/ml. the proxima ...201930633984
impact of sugar composition on meal distribution, longevity, and insecticide toxicity in aedes aegypti.toxic sugar baits (tsbs) are an inexpensive and field-applicable approach to deliver a variety of insecticides to sugar-seeking mosquitoes. we reasoned that carbohydrate chemistry could alter the performance and efficacy of tsbs. in this study, the uptake, distribution, and survival of female aedes aegypti provided with twelve different aqueous sugar meals was recorded. sucrose, a standard control sugar used in mosquito rearing, is always diverted to the ventral diverticulum upon ingestion; but ...201930633897
honeysuckle essential oil as a potential source of ecofriendly larvicides for mosquito control.some plant essential oils have insecticidal properties against mosquitoes and can be harnessed as ecofriendly tools for mosquito control. we conducted bioassays to determine the toxicity of italian honeysuckle (lonicera caprifolium) essential oil and its fractions against larvae of the yellow fever mosquito, aedes aegypti.201930632272
sex and age modulate antennal chemosensory-related genes linked to the onset of host seeking in the yellow-fever mosquito, aedes aegypti.the mosquito aedes aegypti is the primary vector for the fastest growing infectious disease in the world, dengue fever. disease transmission heavily relies on the ability of female mosquitoes to locate their human hosts. additionally, males may be found in close proximity to humans, where they can find mates. host seeking behaviour of both sexes is dependent on adult sexual maturation. identifying the molecular basis for the onset of host seeking may help to determine targets for future vector c ...201930631085
crossing the mucosal barrier: a commensal bacterium gives dengue virus a leg-up in the mosquito midgut.commensal bacteria that colonize the midgut of aedes aegypti mosquitoes can influence the transmission of arthropod-borne viruses. in this issue of cell host & microbe, wu et al. (2019) show that serratia marcescens bacteria secrete enhancin proteins that cleave membrane-bound mucins, thereby facilitating dengue virus infection of midgut epithelial cells.201930629911
economic burden of dengue in indonesia.dengue is associated with significant economic expenditure and it is estimated that the asia pacific region accounts for >50% of the global cost. indonesia has one of the world's highest dengue burdens; aedes aegypti and aedes albopictus are the primary and secondary vectors. in the absence of local data on disease cost, this study estimated the annual economic burden during 2015 of both hospitalized and ambulatory dengue cases in indonesia.201930629593
ocular findings in yellow fever infection.yellow fever virus (yfv) is a reemerging, potentially lethal arboviral disease that has been occurring recently in africa and south america. poor levels of immunization have facilitated the viral spread in southeastern brazil, leading to an unprecedented outbreak that started in late 2016. although human cases have been linked to sylvatic mosquitoes, the concern is that yfv may spread to urban centers infested with aedes aegypti and aedes albopictus mosquitoes and start a true urban cycle.201930629101
adaptation of aedes aegypti to salinity: characterized by larger anal papillae in larvae. 201930618451
epidemic potential of zika virus in australia: implications for blood transfusion safety.zika virus (zikv) is transfusion-transmissible. in australia the primary vector, aedes aegypti, is established in the north-east, such that local transmission is possible following importation of an index case, which has the potential to impact on blood transfusion safety and public health. we estimated the basic reproduction number (r 0 ) to model the epidemic potential of zikv in australian locations, compared this with the ecologically similar dengue viruses (denv), and examined possible impl ...201930618208
larvicidal and pupicidal activities of eco-friendly phenolic lipid products from anacardium occidentale nutshell against arbovirus vectors.aedes aegypti and culex quinquefasciatus are vectors of diseases that constitute public health problems. the discovery of products capable of inhibiting their development which are less harmful to the environment would have a huge impact on vector control. here, natural cashew nut shell liquid (cnsl), technical cnsl, anacardic acid, cardanol, and cardol were isolated from anacardium occidentale and evaluated for larvicidal and pupicidal activity against ae. aegypti and cx. quinquefasciatus under ...201930610586
assessment of combined tools and strategies for aedes aegypti control with low environmental impact.the control of the mosquito vector aedes aegypti l. (diptera: culicidae) is the main action against dengue, chikungunya, and zika. the excessive use of conventional insecticides has promoted the development of other control methods and strategies with lower environmental impact. we evaluated the effectiveness of applying triflumuron 1 ppm and emptying water-filled containers in a field trial in temperate argentina. both control methods were implemented either individually or combined and regular ...201930607607
alternative strategies for mosquito-borne arbovirus control.mosquito-borne viruses-such as zika, chikungunya, dengue fever, and yellow fever, among others-are of global importance. although vaccine development for prevention of mosquito-borne arbovirus infections has been a focus, mitigation strategies continue to rely on vector control. however, vector control has failed to prevent recent epidemics and arrest expanding geographic distribution of key arboviruses, such as dengue. as a consequence, there has been increasing necessity to further optimize cu ...201930605475
laboratory evaluation of repellency of traditional czech homemade repellents against aedes aegypti.in the czech republic, autochtonous transmission of mosquito borne diseases is not common; however, the need for personal protection should not be underestimated. many people still rely on homemade repellents utilizing recipes based on local folk wisdom that are published annually in local czech media. despite minimal disease risk, nuisance biting and potential allergic responses make it essential to evaluate the chemical composition, effect, and duration of four homemade repellents often used a ...201930602280
igg1 and igg4 antibodies against aedes aegypti salivary proteins and risk for dengue infections.dengue virus (denv) is an arbovirus responsible for a significant number of deaths in latin america. this virus is transmitted through the bite of aedes aegypti, the main mosquito vector, and ae. albopictus. during blood uptake, the mosquito injects its saliva into the host to facilitate the feeding process. mosquito saliva contains potent immunogens capable of inducing antibody production directly related to mosquito bite exposure intensity and disease risk. in this study, we first determined t ...201930601814
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