Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the subcellular localization of apomucin and nonreducing terminal n-acetylgalactosamine in porcine submaxillary glands. | antibodies prepared against enzymatically deglycosylated porcine submaxillary gland mucin (apomucin), which were unreactive with native mucin and its partially deglycosylated derivatives, were used to immunolocalize apomucin in situ. electron microscopy of sections of lowicryl k4m-embedded tissue reacted successively with antibodies and protein a-gold complexes showed apomucin exclusively in mucous cells within the rough endoplasmic reticulum, transitional elements of the endoplasmic reticulum, ... | 1988 | 3339018 |
| cytochemical localization of blood group substances in human salivary glands using lectin-gold complexes. | we investigated localization of blood group antigens and their related substances in human labial salivary and submandibular glands by application of a post-embedding cytochemical staining procedure using lectin- or glycoprotein-gold complexes. surgical tissue was obtained from 10 patients. blood group-specific lectins, such as dolichos biflorus agglutinin or helix pomatia agglutinin (group a-specific), griffonia simplicifolia agglutinin-i b4 (group b-specific), and ulex europaeus agglutinin i ( ... | 1988 | 3346537 |
| ultrastructural studies of glycoconjugates in brain micro-blood vessels and amyloid plaques of scrapie-infected mice. | lectin or glycoprotein-gold complexes and samples of scrapie-infected mouse brain embedded in lowicryl k4m were used for ultrastructural localization of glycoconjugates. the lectins tested recognize the following residues: beta-d-galactosyl [rca, ricinus communis agglutinin (aggl.) 120], n-acetyl and n-glycolyl neuraminic acid (lfa, limax flavus aggl.), n-acetyl-d-glucosaminyl and sialyl (wga, wheat germ aggl.), n-acetyl-d-galactosaminyl (hpa, helix pomatia aggl., and dba, dolichos biflorus aggl ... | 1988 | 3348084 |
| lectin-gold labeling of glycoconjugates in normal and brattleboro rat papilla: effect of vasopressin. | some reports suggest that the plasma membrane glycocalyx of collecting duct epithelial cells, as well as interstitial glycoconjugates, may be involved in vasopressin action and urinary concentration. in view of this, we have used the lectin-gold technique to map and quantify helix pomatia lectin (hpl)-binding sites in the inner medulla of kidneys from normal long-evans rats, vasopressin-deficient brattleboro rats, and brattleboro rats treated for up to 5 wk with exogenous vasopressin. the result ... | 1988 | 3348385 |
| glycolipid-lectin interactions: reactivity of lectins from helix pomatia, wisteria floribunda, and dolichos biflorus with glycolipids containing n-acetylgalactosamine. | the autoradiographic detection of 125i-labeled lectins binding to glycolipids on thin-layer chromatograms can be used to rapidly analyze total glycolipid extracts of cells or tissues for specific oligosaccharide structures. the helix pomatia lectin which binds with high affinity to terminal alpha-linked galnac residues did not bind to globoside (terminal beta 1-3galnac) but did bind the ganglioside gm2 and its asialo derivative which have terminal beta 1-4galnac residues. the lectin from dolicho ... | 1988 | 3355161 |
| biosynthesis of the human sucrase-isomaltase complex. differential o-glycosylation of the sucrase subunit correlates with its position within the enzyme complex. | the biosynthesis and maturation of human sucrase-isomaltase (si, ec 3.2.1.48-10), was studied in cultured small intestinal biopsy specimens and mucosa explants. pulse-chase experiments with [35s]methionine revealed one high mannose intermediate of mr = 210,000 (pro-sih) which was processed at a slow rate to an endo h-resistant, mature form of mr = 245,000 (pro-sic). the fully core-glycosylated form (mr = 212,000) was detected only when 1-deoxynojirimycin was added to the culture medium, thus ind ... | 1988 | 3366777 |
| purification of forssman and human blood group a glycolipids by affinity chromatography on immobilized helix pomatia lectin. | a method for the affinity purification of intact glycolipids having nonreducing terminal alpha 1-3 linked n-acetylgalatosamine residues has been developed. this technique relies on the retention of the carbohydrate-binding specificity of immobilized helix pomatia lectin in aqueous solutions of tetrahydrofuran. both forssman glycolipid and a mouse blood group a-active hexaosylceramide were bound by columns of the lectin equilibrated in a solvent containing 95% tetrahydrofuran and 5% water. after ... | 1988 | 3389512 |
| alpha-n-acetylgalactosaminidase deficiency, a new lysosomal storage disorder. | a new lysosomal storage disease with autosomal recessive inheritance is described in two male siblings of 5 1/2 and 4 years of age. clinical manifestations started after 9 months of age with neurological symptoms, followed by progressive psychomotor deterioration. urinary oligosaccharide excretion was abnormal and showed a characteristic pattern on chromatography. enzyme assays showed a profound deficiency of lysosomal alpha-n-acetylgalactosaminidase in cultured fibroblasts, leukocytes and plasm ... | 1988 | 3149698 |
| cytochemical characterization of mouse l1210 leukemia. | mouse lymphatic leukemia l1210 cells were characterized with polyclonal and monoclonal antibodies and lectins. the cells were found to have the phenotype igg-, thy-1.2-, lyt-1-, lyt-2-, asialo-gm1-, tl-, i-ad-, il-2r+, peanut agglutinin+, and helix pomatia lectin +/-. they retained expression of h-2kd and h-2dd. thus, these cells resemble "null" cells. | 1988 | 2466781 |
| detection of b and t cells, with lectins or antibodies, in healthy and bovine leukemia virus-infected cattle. | lectins, polyclonal antibodies and monoclonal antibodies (mabs) were evaluated as markers for bovine lymphocytes obtained from healthy animals and from cattle infected with bovine leukemia virus (blv). in the blood from healthy cattle the proportion of cells identified as t lymphocytes with the lectin helix pomatia (hp) (67.8 +/- 6.2%) using the indirect immunofluorescence technique was similar to the proportion of cells identified by the mabs p5 (66.1 +/- 3.8%) and blt-1 (59.8 +/- 7.1%). the pr ... | 1988 | 2839934 |
| heterogeneity of apical glycoconjugates in kidney collecting ducts: further studies using simultaneous detection of lectin binding sites and immunocytochemical detection of key transport enzymes. | in the search for a functional role for the polarized glycoconjugates of rat collecting duct epithelial cells, the relation between binding of various lectins and expression of cellular transport enzyme profile of the cells was studied. for this purpose, principal and intercalated cells of rat kidney collecting duct were identified by morphological criteria and by their immunocytochemically determined content of (na+ + k+)-atpase and carbonic anhydrase (ca ii), respectively. various n-acetylgala ... | 1988 | 2853698 |
| the reaction of hydroxyurea with oxyhaemocyanin and methaemocyanin of the crayfish astacus leptodactylus and the snail helix pomatia. | the reaction of hydroxyurea with the oxyhaemocyanins of astacus leptodactylus and helix pomatia yielded methaemocyanins which could be regenerated with hydroxylamine. hydroxyurea did not react with astacus methaemocyanin, but quantitatively regenerated helix methaemocyanin under n2. the reaction of hydroxyurea with helix haemocyanin at ph 5.7 under air thus led to a steady state, with an oxyhaemocyanin/methaemocyanin ratio of 2.05:1. | 1988 | 3178776 |
| enteric neuromuscular junctions: comparison of ultrastructural features in different phylogenetic groups. | the enteric neuromuscular junctions of snail (helix pomatia), locust (locusta migratoria migratorioides), cockroach (periplaneta americana), carp (cyprinus carpio) and tench (tinca tinca) were studied by means of different light and electron microscopic methods. the nitroblue tetrazolium staining revealed that the myenteric plexuses of the above species are composed of nerve cells, a network of varicose nerves and nerve bundles. instead of highly organized ganglia, single neurons or small groups ... | 1988 | 3393275 |
| ultrastructural demonstration of maclura pomifera agglutinin binding sites in the membranocystic lesions of membranous lipodystrophy (nasu-hakola disease). | this paper reports three cases of membranous lipodystrophy (nasu-hakola disease) in two families and studies the carbohydrate components of membranocystic lesions in all three cases, using twelve kinds of lectins labelled by horseradish peroxidase (hrp). maclura pomifera agglutinin (mpa), which specifically binds alpha-d-galactose residues, strongly stained typical membranocystic lesions, whereas the other lectins did not. however, helix pomatia agglutinin (hpa), which specifically binds to n-ac ... | 1988 | 3144083 |
| lectin histochemistry of plaques and tangles in alzheimer's disease. | biotinyl derivatives of several lectins and avidin-horseradish peroxidase were used to study the localization of glycoconjugates in amyloid plaques and in neuritic tangles in brains of patients with alzheimer's disease (ad), downs syndrome (ds) and gerstmann-sträussler syndrome (gss). the lectins tested recognize the following residues: beta-d-galactosyl [ricinus communis agglutinin 120, (rca-1) and peanut agglutinin, (pna)]; alpha-d-galactosyl [griffonia simplicifolia agglutinin (gsa)]; alpha-d ... | 1987 | 2955631 |
| the reaction of nitric oxide with copper proteins and the photodissociation of copper-no complexes. | the reactivity with nitric oxide was investigated for a number of type-1, type-2 and type-3 copper proteins azurin from pseudomonas aeruginosa (type-1 copper); bovine superoxide dismutase, diamine oxidase from pig kidney and galactose oxidase from dactylium dendroides (type-2 copper); haemocyanin from helix pomatia (type-3 copper); the blue oxidases ceruloplasmin from pig serum, and ascorbate oxidase from cucurbita pepo medullosa. type-1 copper formed complexes with no in the oxidised state, whi ... | 1987 | 2822126 |
| host cell-induced differences in o-glycosylation of herpes simplex virus gc-1. i. structures of nonsialylated hpa- and pna-binding carbohydrates. | lectins with narrow oligosaccharide specificities were established as probes to study the host cell influence on the biosynthesis of o-linked oligosaccharides of the herpes simplex virus type 1 (hsv-1)-specified glycoprotein c (gc-1). we found that only gc-1 and no other glycoprotein bound to the peanut lectin (pna), with main specificity for gal(beta 1-3)galnac. previously, we have shown that only gc-1 binds to the helix pomatia lectin (hpa), with main specificity for terminal galnac. the o-lin ... | 1987 | 2825412 |
| the carbohydrate moieties of suppressor igg-binding factor released by murine t cells. | the carbohydrate moieties of murine igg-binding factor (igg-bf) were studied using lectins binding n-glycosylated sequences such as concanavalin a (con a), lens culinaris agglutinin (lca), and wheat germ agglutinin (wga), and lectins binding o-glycosylated sequences such as peanut agglutinin (pna) and helix pomatia agglutinin (hpa). sources of igg-bf were: (1) supernatants from t2d4, a t cell hybridoma constitutively producing igg-bf, and (2) factor purified by affinity chromatography on rabbit ... | 1987 | 3500399 |
| glycosylation of developing human glomeruli: lectin binding sites during cell induction and maturation. | expression of cellular glycoconjugates during differentiation of human fetal kidney was studied using fluorochrome-labeled lectins. each lectin revealed a characteristic binding pattern during the phenotypic change of the nephrogenic mesenchyme and during distinct stages of nephron development. the uninduced mesenchymal cells were positive for pisum sativum (psa), concanavalin a (cona), wistaria floribunda (wga), and ricinus communis (rca-i) lectins. however, these lectins failed to react with t ... | 1987 | 3794308 |
| identification of ecdysone 25-o-beta-d-glucopyranoside as a new metabolite of ecdysone in the nematode parascaris equorum. | a major metabolite of [3h]ecdysone injected into adults of the nematode parascaris equorum maintained in vitro for 48 h was secreted into the culture medium. the compound could be hydrolysed with a crude hydrolase preparation from helix pomatia, yielding ecdysone. sufficient quantity of this material for identification was produced by administration of ecdysone to the parasites. the resulting compound was purified by h.p.l.c. and identified as ecdysone 25-o-beta-d-glucopyranoside by n.m.r. spect ... | 1987 | 3435447 |
| ultrastructural localization of helix pomatia lectin-binding sites in mouse lung elastic fibers. | helix pomatia (snail) lectin complexed with colloidal gold (hpl-gold) recognized binding sites on elastic fibers in plastic embedded sections of lung tissue from mice of several ages. deposition of the lectin-gold particles was examined by electron microscopy. structures such as the elastic laminae of pulmonary vessels and elastic fibers throughout the lung was specifically and intensely decorated by the hpl-gold complex and easily visualized. the binding of the hpl-gold particles was primarily ... | 1987 | 3436842 |
| helix pomatia and ulex europeus lectin binding in human breast carcinoma. | formalin fixed, paraffin embedded tissue from 100 consecutive cases of breast carcinoma were studied for binding with helix pomatia (hpa) and ulex europeus (uea1) lectins. serial sections were pretreated with trypsin or neuraminidase to determine the effect of these enzymes on lectin binding. the lectins were visualized by the peroxidase antiperoxidase technique and the cell staining proportion assessed in a semi-quantitative manner under the light microscope. correlating staining with prognosti ... | 1987 | 3309230 |
| glycoconjugates of the human sperm surface: distribution and alterations that accompany capacitation in vitro. | we have studied changes in the binding of fluoresceinated lectins to human sperm during in vitro capacitation. we first determined the surface labeling pattern of viable sperm obtained by the swim-up procedure. sperm were labeled with 100 micrograms/ml fitc-conjugated lectin at 4 degrees c for 30 min. we simultaneously used hoechst stain 33258 as a supravital stain to help differentiate surface from intracellular lectin labeling. of 14 lectins studied, six (phytohemagglutinin-e, concanavalin a, ... | 1987 | 3147937 |
| lectin-binding characteristics of wuchereria bancrofti microfilariae. | the binding of 10 different lectins to the surface of microfilariae of wuchereria bancrofti has been investigated. wheat germ agglutinin (wga) and helix pomatia lectin (hpa) bound specifically to the sheathed microfilariae indicating the presence of n-acetyl-d-glucosamine and n-acetyl-d-galactosamine respectively on the surface. exsheathed microfilariae did not react with any of the lectins. treatment of sheathed microfilariae with proteases resulted in increased binding of wga and hpa. such tre ... | 1987 | 2884837 |
| adp-ribosyltransferase from helix pomatia. purification and characterization. | adp-ribosyltransferases from several higher eukaryotes have been purified and characterized, but little is known about adp-ribosyltransferases in lower eukaryotes. we have purified an adp-ribosyltransferase (ec 2.4.2.30) from helix pomatia. the enzyme has an apparent km of 26.7 microm. optimal conditions for the enzyme reaction are 17.5 degrees c and ph 8. the time course is linear during the first 10 min of the reaction. the enzyme is capable of poly-adp-ribosylation. the most highly purified p ... | 1987 | 3124818 |
| t and b peripheral blood lymphocytes in normal and lymphocytotic sheep. | surface immunoglobulins (sig), peanut agglutinin (pna), spontaneous erythrocyte rosette (e-rosette) and helix pomatia (hp) marker were investigated in normal and bovine leukemia virus (blv)-infected sheep. in normal sheep, 19.3% +/- 4.9 of peripheral blood lymphocytes (pbl) were sig+, whereas 58% +/- 5.69 were pna+, and 19.6 +/- 5.2 were e-rosette forming cells (e-rfc). in blv-induced lymphocytotic sheep, sig+ cells in pbl reached 59.4% +/- 15.06. in the same animals, pna bound to 20.6% +/- 9.69 ... | 1987 | 3040327 |
| cytotoxic activity in the coelomic fluid of the annelid eisenia foetida sav. | the annelid eisenia foetida not only causes hemolysis of red blood cells of several vertebrate species, but also has a toxic effect on a variety of cell types, such as chicken fibroblasts, guinea-pig polymorphonuclear leukocytes and insect hemocytes. however, it has no influence on the vitality of the coelomocytes of lumbricus terrestris and other lumbricides, nor on the hemocytes of the snail helix pomatia, the mussels anodonta cygnea and unio tumidus, free cells of the turbellarian euplanaria ... | 1987 | 3571568 |
| the golgi apparatus in the acinar cells of the developing embryonic pancreas: ii. localization of lectin-binding sites. | the reaction patterns of the golgi apparatus following staining with the lectins concanavalin a (cona), ricinus communis i agglutinin (rca i), and helix pomatia lectin (hpa) were studied in the pancreas acinar cells of rat embryos in the course of cell differentiation from day 13 through day 20 of gestation. the binding reactions were localized by means of pre-embedment incubation of 10-microns-thick cryosections of pancreas tissue, prefixed in a mixture of 4% formaldehyde/0.5% glutaraldehyde, u ... | 1987 | 3578086 |
| litomosoides carinii: characterization of surface carbohydrates of microfilariae and infective larvae. | the carbohydrate moieties of microfilariae (mf) and infective larvae (l3) have been investigated by lectin-binding technique. mf derived from three sources, namely, uteri (in utero), released in vitro from adults and from blood of rodents infected with litomosoides carinii were examined by using fluoresceinated lectins. wheat germ agglutinin (wga) bound to these mf and the binding was inhibited by n-acetyl glucosamine. in addition to wga, concanavalin a (con a) and lentil lectin (lch) bound to i ... | 1987 | 3602835 |
| a new variant of blood group a. apae. | a new variant of blood group a, termed apae, was found as an inherited characteristic in three unrelated white families. apae was distinguished from other a variants by its agglutination and absorption-elution reactions with only certain human anti-a and -a,b serums, by the red cells that reacted with anti-a from helix pomatia without the need for enzyme treatment, by enhancement of the latter reaction with bromelinization, and by the presence in the serum of an anti-a of normal avidity and tite ... | 1987 | 3603659 |
| complete amino-acid sequence of a functional unit from a molluscan hemocyanin (helix pomatia). | from the beta c-hemocyanin (beta c-hc) of the vineyard snail, helix pomatia, the functional unit d (mr approximately equal to 50,000-55,000) was isolated by limited proteolysis and gel chromatography. a small quantity of functional unit d was obtained intact, but the major part in the form of two peptides (mr approximately equal to 43,000 and 10,000, respectively) connected by a disulfide bridge. after reduction and carboxymethylation, these were separated from each other and cleaved by conventi ... | 1987 | 3620107 |
| analysis of urinary aldosterone metabolites in the guinea-pig. | analysis of urinary metabolites of [1, 2-3h]-aldosterone was performed in the male guinea-pig. separation of urinary metabolites was carried out by countercurrent distribution followed by deae-sephadex a-25 column chromatography. a major component was obtained which was both hydrolyzable with sulphatase from helix pomatia and solvolyzable. paper chromatography of freed steroids revealed the presence of at least two components and the major aglycone cochromatographed with 3 beta, 5 alpha-tetrahyd ... | 1987 | 3622385 |
| echinococcus granulosus: occurrence of ecdysteroids in protoscoleces and hydatid cyst fluid. | both free ecdysteroids and hydrolysable polar conjugated ecdysteroids were detected in protoscoleces of echinococcus granulosus from the equine host, and in hydatid cyst fluid from the same source. comparisons were made of hydatid cyst fluid from e. granulosus infections of three intermediate host species: horses, sheep and humans. ecdysone and 20-hydroxyecdysone were identified in both protoscoleces and hydatid cyst fluids by high-performance liquid chromatography monitoring fractions by radioi ... | 1987 | 3627169 |
| differences in glycocalyx composition between cells of the cortical thick ascending limb of henle and the macula densa revealed by lectin-gold cytochemistry. | the glycocalyx composition of cells from the macula densa and the cortical thick ascending limb of henle was examined in rabbit kidney by means of the lectin-gold technique. a quantitative evaluation at the ultrastructural level showed that macula densa cells had a considerably greater affinity for helix pomatia lectin than adjacent cells of the thick ascending limb. wheat germ lectin and concanavalin a bound equally well to both cell types. this difference in plasma membrane glycocalyx composit ... | 1987 | 3631539 |
| neurite-promoting activity from fetal skeletal muscle: partial purification of a high-molecular-weight form. | neurite extension from sensory neuroblasts dissociated from chick embryo dorsal root ganglia can be stimulated by precoating the polylysine culture surface with extracts of skeletal muscle from bovine fetuses. the active factor(s) may be partially purified from cytosolic extracts of muscle by chromatography on sepharose 6b and affinity chromatography on wheat germ agglutinin or helix pomatia agglutinin columns. extract concentrations of 10-50 micrograms protein per 1 ml were active in promoting ... | 1986 | 3701885 |
| [changes in the chemosensitive properties of the central neurons of the snail helix pomatia and of the mouse as affected by opioid peptides]. | in experiments on neurones of the isolated ganglia of the snail and surviving hippocampal slices from mice, studies have been made of the effect of met- and leu-enkephalins on neuronal sensitivity to acetylcholine and norepinephrine. it was shown that in some cases the enkephalins do not affect bioelectrical activity of the neurones, but changed their responses to acetylcholine and norepinephrine. it is concluded that during investigation of the effect of enkephalins on the nervous cells, alongs ... | 1986 | 3716685 |
| insulin-like growth factor-binding protein from human plasma. purification and characterization. | insulin-like growth factor (igf)-binding protein (bp) has been purified from cohn fraction iv of human plasma by acidification, ion exchange to remove endogenous ligands, and affinity chromatography on agarose-igf-ii. the pure protein appeared as a single peak by high performance reverse-phase and gel permeation chromatography (molecular mass, 45-50 kda), but on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gave a major band at 53 kda and a minor band at 47 kda, unreduced, or 43 and ... | 1986 | 3722172 |
| [effect of enkephalins on central neuronal activity in the snail helix pomatia and the white mouse]. | 1986 | 3739467 | |
| changes in the distribution of endothelial surface glycoconjugates associated with altered permeability of brain micro-blood vessels. | lectin-binding sites located on the endothelial cell (ec) surfaces in unaltered, leaking and resorbing micro-blood vessels (mbvs) in cryo-injured cat brain were studied. lectin or glycoprotein-gold complexes and brain samples embedded in hydrophilic resin lowicryl k4m were used. the lectins tested recognize the following residues: beta-d-galactosyl (ricinus communis agglutinin 120, rca and peanut agglutinin, pna), sialyl (limax flavus agglutinin), n-acetyl-d-galactosaminyl (helix pomatia aggluti ... | 1986 | 3739620 |
| histochemistry of glycosaminoglycans in cartilage ground substance. alcian-blue staining and lectin-binding affinities in semithin epon sections. | the critical-electrolyte-concentration staining method using alcian blue (ab) was applied to etched semithin epon-embedded sections. the distribution of various glycosaminoglycans (gags) was studied in hyaline, elastic, cellular and fibrous cartilage obtained from humans and rodents. the staining patterns in semithin sections were found to correspond to those obtained using paraffin-embedded material. lectin histochemistry was performed on consecutive sections. the following peroxidase-labelled ... | 1986 | 3744895 |
| ultrastructural localization of lectin receptors on the luminal and abluminal aspects of brain micro-blood vessels. | lectin- or glycoprotein-colloidal gold complexes were used for detection of specific monosaccharide residues in mouse brain micro-blood vessels (mbvs). the lectins tested recognize the following residues: beta-d-galactosyl (ricinus communis agglutinin-120, rca-1), alpha-n-acetylgalactosaminyl (helix pomatia agglutinin, hpa), alpha-d-mannosyl and alpha-d-glucosyl (concanavalin a, con a), sialoglycoconjugates (limax flavus agglutinin, lfa), n-acetylglucosaminyl and sialyl (wheat germ agglutinin, w ... | 1986 | 3753715 |
| lectin-dependent recognition of foreign cells by hemocytes of the mussel, mytilus edulis. | phagocytosis of human erythrocytes (rbc) by hemocytes of the mussel mytilus edulis was found to be influenced by four heterologous lectins. the effects were examined in the absence of ca++ ions under three experimental conditions: when the lectins were bound to 1) both hemocytes and rbc, 2) only hemocytes, but not to rbc, and 3) only rbc, but not to hemocytes. the lectins used included: albumen gland agglutinin from helix pomatia (hpa), wheat germ agglutinin (wga), ricinus-120 (ric-120) and conc ... | 1986 | 3754847 |
| characterization of surface glycoproteins on schistosoma mansoni adult worms by lectin affinity chromatography. | adult schistosoma mansoni were radiolabeled by direct radioiodination using the bolton-hunter reagent or by metabolic labeling using radioactive hexose precursors. tegumental material was extracted by freeze-thaw or by incubation in the non-ionic detergent nonidet p-40, then applied to chromatography columns containing the following immobilized lectins: con a, lentil lectin, wheat germ agglutinin, soybean agglutinin and the agglutinins from ricinus communis and helix pomatia. sds-page analysis o ... | 1986 | 3755470 |
| hydrolysis of conjugated steroids by beta-glucuronidase from ampullaria and application to the determination of urinary 17-hydroxycorticosteroids. | the usefulness of ampullaria beta-glucuronidase as a reagent for the hydrolysis of steroid glucuronides was examined. the purified enzyme showed an optimum ph of 4, and was stable between ph 4.5 and 7.5. the enzyme from ampullaria and helix pomatia hydrolyzed various artificial substrates and conjugated steroids. affinity of the ampullaria enzyme for 17-hydroxycorticosteroid glucuronide was 7-fold the affinity of the helix pomatia enzyme. hydrolysis of urinary steroid conjugates was completed by ... | 1986 | 3791634 |
| polyethylene glycol-enhanced nephelometric quantification of bovine submaxillary mucin with helix pomatia lectin. | a new principle for quantification of intact mucin has been developed. the method is reproducible, simple, and sensitive at the submicrogram level and can be performed within an hour. the procedure involves incubation of mucin (bovine submaxillary mucin) and polyethylene glycol with the lectin from the snail helix pomatia and the complexes formed are quantitated by nephelometry. polyethylene glycol was found to enhance the affinitin reaction in a dose-dependent fashion. increasing concentrations ... | 1986 | 3944416 |
| duck lymphocytes. i. purification and preliminary observations on surface markers. | high yields of lymphocytes were obtained by centrifuging duck blood, collected into an equal volume of heparinized phosphate-buffered saline, over ficoll-diatrizoate, sg. 1.077, for 25 min at 200 x g. these lymphocytes did not form e rosettes under a variety of conditions, or ea rosettes with sheep erythrocytes sensitized with rabbit antibodies. high proportions (50-80%) of blood and organ (thymus, bursa of fabricius, spleen, cervical lymph node) lymphocytes had surface immunoglobulin (smig). th ... | 1986 | 3944475 |
| lectin histochemistry of scrapie amyloid plaques. | peroxidase-labeled lectins were used for detection of specific monosaccharide residues in amyloid plaques in brains of scrapie-infected mice. the lectins tested recognize the following residues: beta-d-galactosyl (ricinus communis agglutinin 120, rca-1), alpha-d-galactosyl and alpha-d-galactopyranoside (bandeirea simplicifolia aggl., bsa), alpha-d-mannosyl and alpha-d-glucosyl (concanavalin a, con a), n-acetylglucosaminyl and sialyl (wheat germ aggl., wga), sialoglycoconjugates (limulus polyphem ... | 1986 | 3962600 |
| separation of human b lymphocytes on helix pomatia a haemagglutinin into two major fractions differing in responsiveness to t-dependent mitogen (pokeweed mitogen) or antigen (tetanus toxoid). | a b-cell fraction consisting of 70% of cells carrying the b-cell-associated b1 antigen, 15-20% of m1+ non-b cells, and less than 3% of t cells was prepared from the peripheral blood of healthy human donors, previously vaccinated with tetanus toxoid (tt). as assessed by immunofluorescence after treatment with neuraminidase, approximately 40-50% of the b cells had surface structures binding to helix pomatia a haemagglutinin (hp). the cells were separated into three fractions by affinity chromatogr ... | 1986 | 3081997 |
| comparative investigations of latin american trypanosomes with lectins and complement lysis test. | four-days old epimastigote culture forms of different stocks of trypanosoma cruzi from colombia, costa rica and mexico as well of colombian stocks of t. rangeli were tested with 27 lectins and the complement lysis test. while the stocks of t. cruzi and t. rangeli showed common agglutination reactions with the lectins of canavalia ensiformis and pisum sativum, only the stocks of t. cruzi were additionally agglutinated by ricinus communis-120, glycine maxima, helix pomatia, axinella polypoides, ba ... | 1986 | 3085200 |
| the sheep erythrocyte receptor and both alpha and beta chains of the human t-lymphocyte antigen receptor bind the mitogenic lectin (phytohaemagglutinin) from phaseolus vulgaris. | we have studied the interaction of mitogenic lectins such as phytohaemagglutinin (pha) and concanavalin a (con a) with both surface molecules which, by the use of monoclonal antibodies, are known to trigger t-cell mitogenesis. monoclonal antibodies recognizing the t-lymphocyte receptor for antigen (ti) and/or its associated structure, cd3, activate t cells. more recently, a second pathway of activation has been described which involves the sheep erythrocyte binding glycoprotein cd2, a surface mo ... | 1986 | 3085210 |
| the role of phosphate groups in the interaction of human c-reactive protein with galactan polysaccharides. | human c-reactive protein (crp) shows binding specificities for phosphate monoesters, polycations and for several other biological macromolecules lacking these ligands. we report here that the formerly observed interaction of crp with snail galactans, as exemplified by helix pomatia galactan, is not due to a lectin-like carbohydrate-binding reactivity, but, instead that crp obviously binds to phosphate groups that are minor constituents of these polysaccharides. structural analysis of the galacta ... | 1986 | 3086219 |
| lectin histochemistry of papillary and follicular carcinoma of the thyroid gland. | the lectin-binding properties of human follicular and papillary carcinoma were studied histochemically and compared with lectin binding to normal or goitrous thyroid tissue. well-differentiated minimally invasive follicular carcinoma showed a lectin-binding pattern essentially identical to those of the normal thyroid gland and benign adenomatous lesions. overtly invasive follicular carcinoma showed focal reactivity with some lectins that were nonreactive with normal follicular thyroid cells (sol ... | 1986 | 3089192 |
| receptors for erythrogenic toxin a of streptococcus pyogenes on human peripheral blood lymphocytes and other blood cells. | the binding of erythrogenic toxin a (eta) of group a streptococci on human peripheral blood cells was demonstrated light- and electronmicroscopically by labelling with eta-gold. double labelling experiments of lymphocytes and the use of purified t-lymphocytes revealed that the eta-receptor was confined to t cells. besides lymphocytes, monocytes and especially granulocytes and thrombocytes showed a significant eta-gold labelling. the lymphocyte eta-receptor was found to be not sensitive to trypsi ... | 1986 | 3090624 |
| b lymphoblastoid cell lines with normal and defective o-glycosylation established from an individual with blood group tn. | individuals with the tn blood group contain terminal serine/threonine-linked n-acetylgalactosamine residues in their blood cells. this is due to lack of udp-d-galactose: d-n-acetyl galactosamine beta-d-galactosyl transferase from part of their red cells and probably from their leukocytes. we have established b lymphoblastoid cell lines from such an individual by in vitro infection of his lymphocytes with epstein-barr virus. the original line contained a mixture of cells reactive and nonreactive ... | 1986 | 3006838 |
| characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for n-acetylgalactosamine-specific lectins and its identification as g92k or gg. | extracts from herpes simplex virus type 2 (hsv-2)-infected cells were subjected to affinity chromatography with gel-bound helix pomatia lectin (hpa). only one hsv-2-specified glycoprotein was isolated by this procedure and the glycoprotein had an apparent molecular weight of 130 000 (130k). the hpa-binding glycoprotein was genetically mapped, using hsv-1 x hsv-2 intertypic recombinants into the short component of the hsv-2 genome. the mapping position, electrophoretic mobility and the antigenic ... | 1986 | 3007660 |
| in vitro attachment of mono- and oligosaccharides to surface glycoconjugates of intact cells. | we have synthesized glycosylhydrazines of various mono- and oligosaccharides and coupled these to periodate- or galactose oxidase-treated human red cells and k562 erythroleukemia cells. the optimal conditions for this carbohydrate modification of cells have been established. this method makes it possible to specifically elongate oligosaccharide chains of cell surface glycoconjugates with desired carbohydrates. in this way, new antigenic and receptor properties can be conferred to cells, and the ... | 1986 | 3013895 |
| lectin histochemistry applied to human nerves. | histologic sections of normal and pathologically altered human peripheral nerves were stained with a battery of 20 fluorescein isothiocyanate-labeled lectins to determine whether these histochemical reagents could be used to identify peripheral nerve injury. eight plant lectins--from canavalia ensiformis, lens culinaris, triticum vulgare (wheat germ), types e4 and l4 from phaseolus vulgaris, types i and ii from ricinus communis, and wistaria floribunda--were found to bind to normal and pathologi ... | 1986 | 3015069 |
| isolation of glycoproteins of parainfluenza i (sendai) by helix pomatia lectin column. | glycoproteins of sendai virus were successfully isolated on a column of helix pomatia lectin-sepharose 6mb following solubilization with nonidet p-40. this technique can be used as a preparative step for the study of viral glycoproteins. | 1986 | 3021254 |
| [cell surface receptors in lymphoid lines studied using lectins]. | the nature and distribution of some carbohydrate receptors on the cell membrane of transformed human b- and t-derived lymphocyte cell lines (rpmi-1788, daudi, jurkat, molt-4) are studied by ricinus communis agglutinin, soybean agglutinin, helix pomatia lectin, peanut agglutinin and wheat germ agglutinin conjugated with ferritin and colloidal gold. the quality of receptors and the manner of their distribution detected by different lectins are considerably different. | 1986 | 3023007 |
| characterization of the soluble immune complexes that are detected by three different techniques. | three different tests which are based on different principles were used for the detection of soluble immune complexes (ic): (i) a peg precipitation test, which is based on the solubility characteristics of ic; (ii) a solid-phase c1q binding assay, which is based on the complement binding property of ic, and uses peroxidase-linked anti-human igg to detect the bound ic (c1q-elisa); and (iii) the indirect granulocyte phagocytosis test (igpt) which is based on the fc r- and possibly the c3 r-binding ... | 1986 | 3484441 |
| lectin histochemistry of galactose and n-acetyl-galactosamine glycoconjugates in normal gastric mucosa and gastric cancer and the relationship with abo and secretor status. | the histochemical binding of four lectin-peroxidase conjugates to normal human gastric mucosa and gastric carcinoma is described. the lectins were peanut agglutinin (pna) which is specific for galactose residues and soy bean agglutinin (sba), dolichos biflorus agglutinin (dba) and helix pomatia agglutinin (hpa) which are specific for n-acetylgalactosamine. binding of pna to surface mucous cells or normal gastric mucosa occurred in non-secretors but not secretors and was independent of abo blood ... | 1986 | 3794866 |
| glycoconjugate cytochemistry of the rat small intestine using helix pomatia agglutinin and colloidal gold conjugates. | 1986 | 3805962 | |
| preparation of protein colloidal gold complexes in the presence of commonly used buffers. | in the presence of various commonly used buffers, phosphate-buffered saline (pbs), tris-buffered saline (tbs), na-cacodylate, bovine serum albumin and a wide range of cytochemically active proteins (monoclonal and polyclonal igg, concanavalin a, ricinus communis lectin i, helix pomatia lectin, protein a) were complexed to colloidal gold of different particle sizes (6 nm, 9 nm, 22 nm). the resulting complexes were active in cytochemical labelling. complex-formation in the presence of electrolyte ... | 1986 | 3816820 |
| fluorochrome-coupled lectins reveal distinct cellular domains in human epidermis. | the distribution of saccharide moieties in human interfollicular epidermis was studied with fluorochrome-coupled lectins. in frozen sections concanavalin a (con a), lens culinaris agglutinin (lca), ricinus communis agglutinin i (rcai), and wheat germ agglutinin (wga) stained intensively both dermis and viable epidermal cell layers, whereas peanut agglutinin (pna) bound only to living epidermal cell layers. ulex europaeus agglutinin i (ueai) bound to dermal endothelial cells and upper cell layers ... | 1986 | 3512692 |
| biochemical properties of larval excretory-secretory glycoproteins of the parasitic nematode toxocara canis. | toxocara canis larvae, infective to man, secrete a number of antigenic macromolecules into culture medium over prolonged periods of time. these antigens have been collected and characterised with respect to molecular weight by sodium dodecyl sulphate-polyacrylamide gel electrophoresis following extrinsic and intrinsic radiolabelling, or electrophoresis followed by gel staining or immunoblotting. panels of enzymes and lectins have been applied to examine protease sensitivity and carbohydrate comp ... | 1986 | 3515176 |
| isolation and identification of a new major metabolite of diflunisal in man. the sulfate conjugate. | a new metabolite of diflunisal has been identified in volunteers and patients after multiple dose administration. the metabolite was isolated from human urine by silica gel chromatography and was further purified by reversed phase hplc. arylsulfatase from helix pomatia and from aerobacter aerogenes completely hydrolyzed the isolated metabolite to diflunisal, although hydrolysis by bacterial arylsulfatase was extremely slow. electron impact mass spectra for diflunisal and its sulfate conjugate we ... | 1986 | 2868855 |
| hopantenic acid beta-glucoside as a new urinary metabolite of calcium hopantenate in dogs. | the metabolism of calcium hopantenate (hopa) was studied in beagle dogs. after oral administration of 14c-labeled hopa, 25.5% of the administered radioactivity was excreted in the urine within 24 hr, mostly in the form of unchanged drug. the only metabolite, accounting for 4.2% of the radioactivity in the urine, was isolated by hplc. the metabolite was hydrolyzed by the treatment of beta-glucuronidase (helix pomatia), acid phosphatase, or beta-glucosidase. these enzyme activities were not inhibi ... | 1986 | 2877836 |
| regional heterogeneity of glycoconjugate distribution in the glomerulus revealed by lectin-gold cytochemistry and sds-page. | the authors have used sds-page and lectin overlay analysis in parallel with lectin-gold cytochemistry to identify helix pomatia lectin (hpl) binding glycoconjugates in rat kidney glomeruli. previous work revealed hpl binding sites only beneath podocyte foot process bases, where they contact the glomerular basement membrane. it is shown here that after neuraminidase digestion of thin sections of glomeruli before incubation with hpl-gold complexes, the number of hpl binding sites is markedly incre ... | 1986 | 2432793 |
| localization and quantitative distribution of biogenic monoamines in the intestinal tract of locust, snail and carp. | the localization and quantitative distribution of the biogenic monoamines of the intestinal tract has been studied in locusta migratoria, helix pomatia and cyprinus carpio with morphological and biochemical methods. electron microscopically dense-core vesicles of aminergic character were found in the varicose nerve fibres located in the intestinal muscles of all three animal species. intensive green fluorescence characteristic of catecholamines was detectable in both the varicose nerve fibres an ... | 1986 | 20493154 |
| [lectin binding of psoriatic skin]. | the aim of our study was the characterization of epidermal lectin binding pattern in psoriatic vs. non-psoriatic skin in order to reveal possible alterations of the glycocalyx composition of psoriatic keratinocytes. we used fluoroisothiocyanate-labeled lectins of canavalia ensiformis (cona), phaseolus vulgaris (pha), lens culinaris (lca), and helix pomatia (hpa). the binding pattern of psoriatic (involved and non-lesional) skin did not differ from the control samples in cona, pha, and lca. in ps ... | 1986 | 3776279 |
| nerve cells and synaptic connections in the intestinal nerve of the snail, helix pomatia l. an ultrastructural and hrp study. | the ultrastructure of nerve cells and the fine-structural organization of synaptic contacts have been investigated in the intestinal nerve in the snail helix pomatia. three types of nerve cells, occurring singly or in groups, can be distinguished on the basis of the ultrastructure of their perikaryon and content of granules. the peripheral output of these nerve cells has been verified by retrograde cocl2 and nicl2 staining. both axosomatic and axo-axonic specialized synaptic contacts occur in th ... | 1985 | 3986882 |
| characterization of candida antigens by crossed-immunoaffinoelectrophoresis. | the antigens of three candida albicans strains (3153 a, 3156 b and cbs 1905) and one c. tropicalis strain were studied by means of crossed-immunoaffinoelectrophoresis with the corresponding polyvalent antisera. most antigens (from 63.8% to 77.7% depending on the strain) were bound to concanavalin a-sepharose and about 20% to blue cibacron-sepharose for all the strains tested. free concanavalin a, wheat germ lectin-sepharose and helix pomatia lectin-sepharose revealed differences between c. albic ... | 1985 | 2409611 |
| alterations in glomerular lectin binding sites of human kidney as detected by fluorescence microscopy. | different lectins were used to study frozen sections of kidney samples showing alterations in routine immunofluorescence studies. arachis hypogaea agglutinin (peanut lectin, pna), lacking binding sites in normal glomeruli, bound to the glomeruli in two of the five samples studied, giving a granular fluorescence pattern. concomitantly with the appearance of pna-binding, binding sites for wheat germ agglutinin (wga) appeared to be lost at glomeruli. furthermore, changes in the expression of glomer ... | 1985 | 2415488 |
| lyophilization of protein-gold complexes. | conditions for dialysis, freezing and lyophilization of protein-gold complexes were established. lyophilized complexes formed by colloidal gold with staphylococcal protein a, with helix pomatia lectin and with horseradish peroxidase retained their original staining properties when reconstituted after several months of storage. | 1985 | 2416715 |
| tn epitopes, immunoreactive with ordinary anti-tn antibodies, on normal, desialylated human erythrocytes and on thomsen-friedenreich antigen isolated therefrom. | hybridoma generation, using specifically, maximally desialylated human blood group o erythrocytes (t rbc) as immunogen, and biochemical studies suggested the presence of immunogenic tn epitopes. galnac alpha-o, on t rbc. we therefore investigated by immunochemical means whether or not tn-specific epitopes immunoreactive with anti-tn antibodies present in ordinary human sera occur on t rbc and on thomsen-friedenreich (t) antigen prepared from them. we did detect the tn epitope with such antibodie ... | 1985 | 2417112 |
| gas chromatographic/mass spectrometric analysis of 19-nortestosterone urinary metabolites in man. | a sensitive and highly specific method based on capillary column gas chromatography/mass spectrometry has been developed for the detection of 19-nortestosterone (17 beta-hydroxy-4-estren-3-one) metabolites in urine. after intramuscular administration of 19-nortestosterone decanoate to man, urine samples were collected during several days and treated with helix pomatia digestive juice. the free steroids were extracted and converted into o-methyl-oxime-trimethylsilyl or the trimethylsilyl ether de ... | 1985 | 3160396 |
| lectin-binding to hemocytes of mytilus edulis. | lectin receptors for wga, ricinus 60, ricinus 120, and for the agglutinin from the albumin gland of helix pomatia were detected on the surface of mytilus hemocytes by an agglutination assay. when hemocyte monolayers on slides were incubated with these lectins, strong rosette formation was obtained after addition of human erythrocytes. the lowest lectin concentration which caused formation of rosettes was determined to be 5 micrograms/ml of wga or of helix agglutinin, and 20 micrograms/ml of rici ... | 1985 | 3839466 |
| phylogenetic aspects of cardiac hormones as revealed by immunocytochemistry, electronmicroscopy, and bioassay. | a hormone family of cardiac peptides has recently been isolated and biochemically and pharmacologically characterized by the relaxation of vascular smooth muscle, diuretic and natriuretic activities. the cardiac hormones are stored in specific granules of the atrial myoendocrine cells. since data is available only from mammals (rat, pig, man) we started a phylogenetic study by investigating representatives of the higher vertebrate classes (birds, reptiles, amphibians, bony fish) as well as an in ... | 1985 | 3841692 |
| [lectin-inducible cell cytotoxicity and the production of the cytotoxic factor of natural killer cells during immobilization stress in mice]. | mice exposed to immobilization stress manifested a dramatic lowering of the activity of normal killers against yac-1 tumor as well as against the lectin-induced cellular cytotoxicity mediated by helix pomatia agglutinin and detected by the cytolytic test against xenogeneic tumor cells k 562. exposure to stress did not provoke any change in the production of cytotoxic factor of normal killers, whereas dexamethasone (5 x 10(-7) m) in vitro inhibited the production of this factor. | 1985 | 3860263 |
| biosynthesis of the major human red cell sialoglycoprotein, glycophorin a. o-glycosylation. | the biosynthesis of the major human red cell sialoglycoprotein, glycophorin a, was studied in the erythroleukemia cell line k562 with emphasis on o-glycosylation. the cells were pulse-chase labeled with [35s] methionine, and either directly immune precipitated with anti-glycophorin a antiserum or detergent-solubilized extracts first passed through columns containing the n-acetylgalactosamine-specific lectin from helix pomatia or the glucose/mannose specific lectin from lentil beans. from the sug ... | 1985 | 3861610 |
| mutagenicity of beta-glucuronidase in the ames test. | the enzyme preparation beta-glucuronidase/arylsulphatase from helix pomatia (boehringer) caused base-pair substitutions in salmonella typhimurium ta100 and ta1535 strains within the dose range of 0.50-50 microliter per plate. no effect was observed in the ta98 strain. the presence of s9 mix did not substantially affect the mutagenic potential of beta-g. the number of induced revertants decreased continually from experiment to experiment carried out in the course of 12 weeks. | 1985 | 3892278 |
| horseshoe crab (limulus polyphemus) lectin but not garden snail (helix pomatia) lectin elicits insulin-like activities in vitro. | lectins from the horseshoe crab (limulus polyphemus) and the garden snail (helix pomatia) were tested for insulinomimetic activities in isolated rat epididymal adipocytes. the sialic acid binding horseshoe crab lectin suppressed epinephrine-induced lipolysis and augmented lipogenesis from d-[3-3h]-glucose while the n-acetylgalactosamine binding snail lectin was inactive. the results suggest that the insulin receptor on rat adipocytes contains sialic acid in its carbohydrate moiety but does not p ... | 1985 | 3893876 |
| intermediate filaments in non-neuronal cells of invertebrates: isolation and biochemical characterization of intermediate filaments from the esophageal epithelium of the mollusc helix pomatia. | to screen invertebrate tissues for the possible expression of intermediate filaments (ifs), immunofluorescence microscopy with the monoclonal antibody anti-ifa known to detect all mammalian if proteins was used (pruss, r. m., r. mirsky, m. c. raff, r. thorpe, a. j. dowding, and b. h. anderton. 1981. cell, 27:419-428). in a limited survey, the lower chordate branchiostoma as well as the invertebrates arenicola, lumbricus, ascaris, and helix pomatia revealed a positive reaction primarily on epithe ... | 1985 | 3894375 |
| lectin-gold cytochemistry reveals intercalated cell heterogeneity along rat kidney collecting ducts. | the lectin-gold technique was used to detect helix pomatia and dolichos biflorus lectin binding sites directly on semithin and thin sections of rat kidney collecting ducts. intercalated cell apical plasma membranes and the membranes of apical cytoplasmic vesicles were heavily labeled in the cortex and outer stripe of the outer medulla but were negative or very weakly labeled in the inner stripe and inner medulla. in contrast, clear cell apical membranes were labeled along the entire length of th ... | 1985 | 3919593 |
| binding of lectins to streptococcus mitis cells. studies of the specificity of ligand mediated aggregation. | previous studies have shown that the mechanism of spontaneous aggregation of streptococcus mitis atcc 903 depends on a lectin-ligand type interaction. to study the specificity of the ligand, the binding of a number of lectins of different sugar specificities to the surface of untreated, trypsin and beta-galactosidase-treated bacteria was studied by assessing aggregation. untreated bacteria were rapidly aggregated by concanavalin a (con a), wheat-germ agglutination (wga) and helix pomatia lectin ... | 1985 | 3920867 |
| further studies on the surface saccharides in trichomonas vaginalis strains by fluorescein-conjugated lectins. | fluorescence emitted by individual cells of several trichomonas vaginalis strains, nearly all of which were cloned, incubated with fluorescein-conjugated lectins in the absence (experimental) or presence (control) of inhibitory sugars, or else in phosphate-buffered saline alone (autofluorescence) was measured with a leitz mpv compact microfluorometer. irrespective of whether the organisms were postfixed in formalin or glutaraldehyde, the relative fluorescence emitted by the cells was closely com ... | 1985 | 3927600 |
| patterns of urinary oestrogen excretion in female golden lion tamarins (leontopithecus rosalia). | daily urine samples were collected from 5 female golden lion tamarins (leontopithecus rosalia) over a period of 3 or more months, and urinary oestrogen concentrations were determined by radioimmunoassay. four females exhibited regular patterns of oestrogen excretion, with a peak-to-peak periodicity of 19.6 +/- 1.4 days. levels of oestrogen excretion tended to vary between, but not within, individual females. post-partum oestrogen patterns included periods of clear oestrogen cyclicity before conc ... | 1985 | 3934366 |
| biochemical and functional characteristics of the human leukocyte membrane antigen family lfa-1, mo-1 and p150,95. | the human leukocyte function-associated (lfa-1) antigen, the monocyte differentiation antigen mo-1 which is characterized as the c3bi receptor and the glycoprotein p150,95 are characterized biochemically. immunoprecipitations carried out with 6 different monoclonal antibodies (mab) against lfa-1 indicated that four mab (spv-l1, spv-l5, spv-l7 and spv-l11) were directed against the alpha chain, whereas mab clb54 and mhm-23 were found to react with the common beta chain of lfa-1, mo-1 and p150,95. ... | 1985 | 2933266 |
| protein blotting: detection of proteins with colloidal gold, and of glycoproteins and lectins with biotin-conjugated and enzyme probes. | methods to detect "native" proteins immobilized on nitrocellulose membranes in spot tests or on blots prepared from polyacrylamide slab gels after electrophoretic separation are described. gold sols were found to be useful as general stains for proteins: they are polychromatic, yield an indelible record, and are complementary to india ink as protein stains because these two stains have different sensitivities for a number of proteins tested. for detection of wheat germ lectin (wgl)-binding glyco ... | 1985 | 2984955 |
| different populations of herpes simplex virus glycoprotein c discriminated by the carbohydrate-binding characteristics of n-acetylgalactosamine specific lectins (soybean and helix pomatia). brief report. | from the herpes simplex virus specified glycoprotein c two fractions were isolated with affinity either for helix pomatia lectin (hpa) or soybean lectin (sba). the data indicated that hpa and sba, despite their mutual main specificity for n-acetylgalactosamine, recognize structurally different gc populations. | 1985 | 2994599 |
| presence of the tn antigen on hematopoietic progenitors from patients with the tn syndrome. | the tn syndrome is an acquired clonal disorder characterized by the exposure of a normally hidden determinant, the tn antigen, on the surface of human erythrocytes, platelets, granulocytes, and lymphocytes. two distinct populations, tn positive (tn+) and tn negative (tn-), of mature hemopoietic cells are present in tn patients. to determine whether the tn antigen is already expressed on erythroid, myeloid, and pluripotent progenitors, light-density mononuclear blood cells from two patients with ... | 1985 | 3973016 |
| a new surface marker on equine peripheral blood lymphocytes. i. subpopulations of lymphocytes with receptors for helix pomatia a hemagglutinin (hp). | untreated and neuraminidase-treated equine peripheral blood lymphocytes were analysed for binding of the a hemagglutinin of the snail helix pomatia (hp). for optimal staining by direct immunofluorescence, the concentration of neuraminidase had to be increased as compared to that needed for other species. moreover, higher concentrations of hp were required for optimal staining of equine lymphocytes as compared to lymphocytes from other species. even so, the maximal number of equine lymphocytes ex ... | 1985 | 3976169 |
| a new surface marker on equine peripheral blood lymphocytes. ii. characterization and separation of purified blood lymphocytes with receptors for helix pomatia a hemagglutinin (hp). | in a preceding report we have shown that the lectin helix pomatia a hemagglutinin (hp) binds to two subpopulations of neuraminidase-treated equine peripheral blood lymphocytes (pbl), constituting about 20% and 75% of pbl, respectively. the aim of the present study was to further characterize these hp+ cells in regard to other surface markers such as receptors for guinea pig erythrocytes (gpr+ cells), membrane-bound immunoglobulins (sig+ cells), receptors for activated complement (c3r+ cells) and ... | 1985 | 3976170 |
| ontogenetic analysis of some surface markers on pig lymphocytes using fluorescence-activated cell sorter. | surface markers were demonstrated on pig lymphocytes using anti-t cell-igg and anti-helix pomatia (hp) igg during prenatal and postnatal development. a fluorescence-activated cell sorter analysis of t-cell surface markers was accompanied by an image analysis to prove the association of t antigenic determinants with the plasma membrane only. we found development-dependent changes in both anti-t cell and hp surface markers in both primary and secondary lymphatic organs. the number of t-positive (t ... | 1985 | 4007711 |
| identification of a novel ganglioside on erythrocytes with blood group cad specificity. | the blood group cad antigen is a carbohydrate structure well characterized on the sialoglycoproteins of the red cell membrane from some rare individuals (blanchard, d., cartron, j. p., fournet, b., montreuil, j., van halbeck, h., and vliegenthart, j.f.g. (1983) j. biol. chem. 258, 7691-7695). however, protease treatment of whole cells did not destroy their antigenic activity which indicated that glycolipid might also be involved in the antigenic reaction. a crude ganglioside fraction was prepare ... | 1985 | 4008478 |
| reactivity of five n-acetylgalactosamine-recognizing lectins with preimplantation embryos, early postimplantation embryos, and teratocarcinoma cells of the mouse. | the expression of receptors for n-acetylgalactosamine-recognizing lectins, namely helix pomatia agglutinin (hpa), sophora japonica agglutinin (sja), bauhinia purpurea agglutinin (bpa), vicia villosa agglutinin (vva), and wistaria floribunda agglutinin (wfa) was studied in early mouse embryos and teratocarcinoma cells. each of these lectins as well as dolichos biflorus agglutinin (dba) bound differently to early embryonic cells, with the exception of vva and wfa which showed indistinguishable rea ... | 1985 | 4018458 |
| interaction of lectins with membrane receptors on erythrocyte surfaces. | the interactions of human genotype ao erythrocytes (red blood cells) (rbcs) with n-acetylgalactosamine-reactive lectins isolated from helix pomatia (hpa) and from dolichos biflorus (dba) were studied. binding curves obtained with the use of tritium-labeled lectins showed that the maximal numbers of lectin molecules capable of binding to human genotype ao rbcs were 3.8 x 10(5) and 2.7 x 10(5) molecules/rbc for hpa and dba, respectively. the binding of one type of lectin may influence the binding ... | 1985 | 4019586 |
| interaction energies in lectin-induced erythrocyte aggregation. | two n-acetylgalactosamine-reactive lectins, helix pomatia (hpa) and dolichos biflorus (dba), were used to study the energies involved in cell-cell interactions through the specific binding of these lectins to their membrane receptors on genotype ao human erythrocytes (red blood cells) (rbcs). the energy required to dissociate a unit of aggregated membrane area (gamma d) of two rbcs bridged by lectin molecules was determined from the shear force needed to dissociate two-cell aggregates in a flow ... | 1985 | 4019587 |
| localization of binding sites for concanavalin a, ricinus communis i and helix pomatia lectin in the golgi apparatus of rat small intestinal absorptive cells. | binding sites for concanvalin a (con a), ricinus communis i agglutinin (rca i), and helix pomatia lectin (hpa) were localized in the golgi apparatus of rat small intestinal absorptive cells. a preembedment technique, a modification of the one originally used by bernhard and avrameas (exp cell res 64:232, 1971), was employed, with horse-radish peroxidase being used for cytochemical visualization. incubations were performed on 10 microns thick cryosections of duodenal segments that were fixed in a ... | 1985 | 4020101 |
| the importance of tumor markers in oral pathology. ii. cell membrane and cytoplasmic antigens as tumour markers. | important tumour markers in tumours of the oral mucosa and salivary glands are intermediate filaments of cytoskeleton, oncofetal and proliferative antigens, lectin receptors and blood group substances, enzymes, metalloproteins and viral antigens. the special occurrence of the following tumour markers was demonstrated: keratin, vimentin, carcinoembryonic antigen (cea), tissue polypeptide antigen (tpa), lectins (helix pomatia antigen = hpa, peanut agglutinin = pna), thomsen-friedenreich-antigen, b ... | 1985 | 4040631 |