Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| pcr diagnosis of infections with different species of opisthorchiidae using a rapid clean-up procedure for stool samples and specific primers. | infections with the opisthorchiid liver flukes clonorchis sinensis, opisthorchis viverrini, and opisthorchis felineus cause serious health problems in endemic areas of southeast asia and countries of the former soviet union. chronic infections--even with low worm burdens--may lead to the development of fatal cholangiocarcinoma and related symptoms. a more sensitive diagnosis is needed since the tiny eggs of the worms are often not seen in microscopic examinations of stool samples, especially in ... | 2007 | 17061114 |
| clonorchis sinensis: development and evaluation of a nested polymerase chain reaction (pcr) assay. | clonorchis sinensis is a fish-borne trematode endemic to east asia, which infects over 35 million people globally. in the study described here, we developed a nested polymerase chain reaction (pcr) method for the specific and reliable detection of c. sinensis. the primers designed from the nucleotide sequence data derived in this study were evaluated for their specificity and sensitivity for the detection of c. sinensis. the specific amplification products were obtained only with c. sinensis and ... | 2007 | 17067580 |
| evaluation of the in vivo activity of tribendimidine against schistosoma mansoni, fasciola hepatica, clonorchis sinensis, and opisthorchis viverrini. | we examined the in vivo activity of tribendimidine against selected trematodes. a single 150-mg/kg dose of tribendimidine achieved a 99.1% reduction of clonorchis sinensis in rats. a 400-mg/kg dose of tribendimidine reduced opisthorchis viverrini in hamsters by 95.7%. high doses of tribendimidine showed no activity against schistosoma mansoni and fasciola hepatica. | 2007 | 17194822 |
| cytokine expression in hamsters experimentally infected with opisthorchis viverrini. | the cytokine mrna expression of il-12, ifn-gamma, tgf-beta, il-4, and il-10 were investigated in spleen, liver and mesenteric lymph nodes (mln) in hamsters experimentally infected with opisthorchis viverrini. animals were infected with 5, 25 or 100 metacercariae (mc) and examined by rt-pcr and real-time pcr at 2 weeks, 2 and 6 months after infection. the cytokine expression was compared using hprt. the il-12 was significantly expressed at 2 weeks in the liver of the 5- and 25-mc-infected groups. ... | 2007 | 17266743 |
| evidence of a species complex within the food-borne trematode opisthorchis viverrini and possible co-evolution with their first intermediate hosts. | the food-borne trematodes, opisthorchis viverrini, o. felineus and clonorchis sinensis, have long been recognized as the cause of major human health problems, with an estimated 40 million infected persons. of the three species of liver fluke, only o. viverrini is classified as a type 1 carcinogen because of its role as an initiator of chronic inflammation and the subsequent development of cholangiocarcinoma. at present, there are no techniques for the early diagnosis of cholangiocarcinoma and it ... | 2007 | 17275001 |
| early stage biliary and intrahepatic migration of opisthorchis viverrini in the golden hamster. | in thailand, infection with the liver fluke opisthorchis viverrini is a serious health problem, with over 8.6 million human infections each year. early stage biliary intrahepatic migration and infection of o. viverrini in the syrian golden hamster were used to study the growth and development of this fluke. fifty metacercariae of o. viverrini were introduced into each hamster by gastric intubation. worms were found to migrate rapidly from the stomach to the gall bladder and hepatic duct, where t ... | 2007 | 17381865 |
| increased tff1 trefoil protein expression in opisthorchis viverrini-associated cholangiocarcinoma is important for invasive promotion. | aims: cholangiocarcinoma (cca) is a poor prognosis cancer that presents with metastatic disease. this cancer expresses muc5ac, a mucin which normally co-expresses with trefoil factor family 1 (tff1) protein. tff1 is a signalling protein that can activate epithelial cell invasion and has been considered as a metastasis stimulating agent. the aim of this study was to determine the co-expression of tff1 and muc5ac in cca tissues and examine the activity of tff1 for stimulating the invasive property ... | 2007 | 17397518 |
| parasitic infestations of the biliary tract. | parasitic infestations of the biliary tract are a common cause of biliary obstruction in tropical countries and can lead to such serious complications as cholangitis and cholangiocarcinoma. endoscopic therapy has helped in the management of biliary complications caused by these parasites. ascaris lumbricoides organisms, which normally reside in the jejunum, are actively motile and can invade the papilla, thus migrating into the bile duct and causing biliary obstruction. endoscopic retrograde cho ... | 2007 | 17418062 |
| parasitic diseases of the biliary tract. | objective: parasites residing in the biliary tree include clonorchis sinensis, opisthorchis viverrini, opisthorchis felineus, and fasciola hepatica. they are willowy, leaf-like, flat flukes dwelling in the bile ducts and gallbladder. human ascarides, ascaris lumbricoides, dwelling in the small intestine, inadvertently migrate into the bile ducts and cause biliary obstruction. the purpose of this article is to illustrate typical imaging findings of liver fluke infection and biliary ascariasis. co ... | 2007 | 17515382 |
| the potential usefulness of the modified kato thick smear technique in the detection of intestinal sarcocystosis during field surveys. | a total of 479 stool specimens were collected from rural communities of ubon ratchathani province, thailand and examined by two techniques: the modified kato thick smear and the direct smear. the prevalence of opisthorchis viverrini (14.8%), hookworm (10.2%), sarcocystis spp (4.6%), taenia spp (2.9%), strongyloides stercoralis (2.1%), giardia lamblia (1.2%), echinostoma spp (0.6%), ascaris lumbricoides (0.4%), entamoeba histolytica (0.2%), chilomastix mesnili (0.2%) and endolimax nana (0.2%) wer ... | 2007 | 17539271 |
| the role of helminth infections in carcinogenesis. | this review examines the significant literature on the role of helminth infections in carcinogenesis. both parasitic infections and cancer have complex natural histories and long latent periods during which numerous exogenous and endogenous factors interact to obfuscate causality. although only two helminths, schistosoma haematobium and opisthorchis viverrini, have been proven to be definitely carcinogenic to humans, others have been implicated in facilitating malignant transformation. the known ... | 2007 | 18063098 |
| artemisinins and synthetic trioxolanes in the treatment of helminth infections. | helminthiases, including schistosomiasis and food-borne trematodiasis, affect millions of people. just a few drugs are used for the treatment and control of these diseases. we review recent in-vitro and in-vivo observations with the artemisinins and synthetic trioxolanes against major trematode infections, update clinical findings, and discuss the potential impact that artemisinin-based combination therapy might have on trematode infections in settings where malaria and helminthiases are co-ende ... | 2007 | 17975411 |
| discrimination of opisthorchis viverrini from haplorchis taichui using coi sequence marker. | this study aimed to discriminate infections of two common fish-borne trematodes in thailand, opisthorchis viverrini from haplorchis taichui, based on mitochondrial cytochrome c oxidase subunit i (coi) gene. designed primers (coi-ov-hap f&r primers) amplified partial coi fragments of o. viverrini and h. taichui with high sensitivity in different developmental stages (adult, metacercaria, and egg). polymerase chain reaction (pcr) amplicons were generated with low genomic dna concentration ( approx ... | 2007 | 17574199 |
| gene discovery for the carcinogenic human liver fluke, opisthorchis viverrini. | cholangiocarcinoma (cca)--cancer of the bile ducts--is associated with chronic infection with the liver fluke, opisthorchis viverrini. despite being the only eukaryote that is designated as a 'class i carcinogen' by the international agency for research on cancer, little is known about its genome. | 2007 | 17587442 |
| apoptosis-related gene expressions in hamsters re-infected with opisthorchis viverrini and re-treated with praziquantel. | our objective was to reveal whether host immune response in hamster opisthorchiasis post-praziquantel treatment could induce apoptotic cell death in inflammatory cells. we, therefore, investigated apoptosis-related gene expression in hamsters re-infected with opisthorchis viverrini (ov) and re-treated with praziquantel. hamsters were re-infected with ov metacercariae then re-treated with praziquantel. the expression of apoptosis-related genes (i.e. apoptosis gene bcl-2 associated protein x [bax] ... | 2007 | 17851691 |
| parasitic infestation and nutritional status among schoolchildren in vientiane, lao pdr. | to investigate the prevalence of intestinal parasitic infestation among school-aged children residing in vientiane capital city; and to assess the impact of its infestation on nutrition status of those children. | 2007 | 17854455 |
| high prevalence of liver and intestinal fluke infections among residents of savannakhet province in laos. | the prevalence of liver and intestinal fluke infections was surveyed on residents of savannakhet province, laos. fecal specimens were collected from a total of 981 residents in 4 mekong riverside villages and examined by the kato-katz thick smear technique. the results revealed that the overall helminth egg positive rate was 84.2%, and the positive rate for small trematode eggs, including opisthorchis viverrini, heterophyids, or lecithodendriids, was 67.1%. to obtain adult flukes, 38 small trema ... | 2007 | 17876167 |
| the elisa-based detection of anti-opisthorchis viverrini igg and igg4 in samples of human urine and serum from an endemic area of north-eastern thailand. | the levels of correlation between the number of opisthorchis viverrini eggs excreted in the faeces and levels of anti-opisthorchis igg and igg(4) in the serum and urine (as indicated by absorbances in elisa) have recently been evaluated in north-eastern thailand. the 225 subjects investigated in detail, all of whom came from an endemic village in chaiyaphum province, were selected on the basis of the numbers of o. viverrini eggs that they were excreting. elisa based on a crude antigen extract of ... | 2007 | 17877877 |
| epidemiology of opisthorchis viverrini in a rural district of southern lao pdr. | in lao pdr, detailed investigations on opisthorchis viverrini are scarce. the objective was to document epidemiological features of o. viverrini infections in a highly endemic district. a random sample was selected (13 villages, 15 households/village, all household members aged >6 months). clinical examinations, short interviews and stool examinations (kato-katz technique) were performed. fish samples were dissected for the presence of metacercaria. in total, 814 persons were enrolled (median ag ... | 2007 | 16828134 |
| opisthorchis viverrini: identification of a glycine-tyrosine rich eggshell protein and its potential as a diagnostic tool for human opisthorchiasis. | a cdna encoding a novel eggshell protein (ovesp) with high-glycine (49.2%) and -tyrosine (27.8%) content was cloned from the human liver fluke opisthorchis viverrini. in the adult parasite, the rna products of the ovesp gene are limited to the vitelline follicles. they have a size of 800 nucleotides and are already present in 2-week-old juveniles. immune sera of hamsters, experimentally infected, and humans, naturally infected with o. viverrini, detect bacterially expressed recombinant ovesp (ro ... | 2006 | 16876169 |
| evaluation of pcr based coprodiagnosis of human opisthorchiasis. | in this study, a recently developed pcr test for the detection of opisthorchis viverrini in human faecal samples was evaluated using two parasitological methods as references. during a survey of foodborne trematodes (fbt) in the vientiane province, lao pdr, 85 samples were collected and evaluated for fbt eggs by the kato katz (kk) technique, the formalin ethyl acetate concentration technique (fect) and a pcr analysis for the distinction between o. viverrini and other fbt. the two parasitological ... | 2006 | 16253202 |
| opisthorchis viverrini: influence of maternal infection in hamsters on offspring infected with homologous parasite and their igg antibody response. | we investigated the influence in hamsters of a maternal opisthorchis viverrini infection on their offspring infected with homologous parasites and the kinetics of the o. viverrini-specific igg antibody responses. no significant difference (p > 0.05) was found in the specific igg antibody response and the number of o. viverrini eggs per gram feces (epg) between infected offspring from infected mothers and infected offspring from uninfected mothers. a significant difference (p < 0.05) of epg per w ... | 2006 | 16472806 |
| altered gene expression in opisthorchis viverrini-associated cholangiocarcinoma in hamster model. | cholangiocarcinoma (cca) induced by liver fluke (opisthorchis viverrini, ov) infection is one of the most common and serious disease in northeast thailand. to elucidate the molecular mechanism of cholangiocarcinogenesis induced by ov infection, we employed a hamster model of cca induced by ov and n-nitrosodimethylamine and analyzed candidate genes involved in cca using fluorescence differential display-pcr. of 149 differentially amplified bands we identified, the upregulation of 23 transcripts a ... | 2006 | 16550611 |
| glycobiological study of adult opisthorchis viverrini: characterization of n-linked oligosaccharides. | 2006 | 16567007 | |
| inos-dependent dna damage via nf-kappab expression in hamsters infected with opisthorchis viverrini and its suppression by the antihelminthic drug praziquantel. | inflammation-mediated dna damage triggered by opisthorchis viverrini (ov) infection is a major risk factor of cholangiocarcinoma (cca). we have recently reported that nitrative and oxidative dna damage participates in cca development caused by repeated infection with ov [pinlaor et al., carcinogenesis 2004; 25:1535-42]. therefore, to clarify the preventive effect of the antihelminthic drug praziquantel against cholangiocarcinogenesis, we assessed the effect of this drug on nitrative and oxidativ ... | 2006 | 16570287 |
| oxidative and nitrative dna damage in animals and patients with inflammatory diseases in relation to inflammation-related carcinogenesis. | infection and chronic inflammation are proposed to contribute to carcinogenesis through inflammation-related mechanisms. infection with hepatitis c virus, helicobacter pylori and the liver fluke, opisthorchis viverrini (ov), are important risk factors for hepatocellular carcinoma (hcc), gastric cancer and cholangiocarcinoma, respectively. inflammatory bowel diseases (ibds) and oral diseases, such as oral lichen planus (olp) and leukoplakia, are associated with colon carcinogenesis and oral squam ... | 2006 | 16606333 |
| 8-nitroguanine, a product of nitrative dna damage caused by reactive nitrogen species: formation, occurrence, and implications in inflammation and carcinogenesis. | the authors review studies on 8-nitroguanine (8-no(2)-g) formed by reactions of guanine, guanosine, and 2 - deoxyguanosine, either free or in dna or rnawith reactive nitrogen species (rns) generated from peroxynitrite, the myeloperoxidase-h(2)o(2)-nitrite system, and others. use of antibodies against 8-no(2)-g has revealed increased formation of 8-no(2)-g in various pathological conditions, including rna virus-induced pneumonia in mice, intrahepatic bile ducts of hamsters infected with the liver ... | 2006 | 16771693 |
| gene expression profiling defined pathways correlated with fibroblast cell proliferation induced by opisthorchis viverrini excretory/secretory product. | to investigate the mechanism of fibroblast cell proliferation stimulated by the opisthorchis viverrini excretory/secretory (es) product. | 2006 | 16773716 |
| clonorchis sinensis and opisthorchis viverrini: development of a mitochondrial-based multiplex pcr for their identification and discrimination. | we report a single, one-step pcr approach for detection and discrimination of clonorchis sinensis and opisthorchis viverrini in different life-stage forms (adults, metacercariae, and eggs) from fish intermediate hosts and from infected patients. primers designed for species-specific pcr, amplifying portions of the mitochondrial (mt) genome, were also suitable for a multiplex pcr. the latter was a single, one-step reaction under high stringency conditions, using simultaneously 2 pairs of primers ... | 2006 | 16310774 |
| enzyme markers to identify and characterize opisthorchis viverrini in thailand and lao pdr. | we conducted an allozyme electrophoretic study to explore potential enzyme markers to distinguish opisthorchis viverrini in thailand and lao pdr. twenty-eight enzymes encoding presumptive 32 loci were established. the enzymes glucose-6-phosphate dehydrogenase and pyruvate kinase were diagnostic between two geographically separate isolates from thailand. twelve enzymes, ie, aconitate hydratase, aldolase, creatine kinase, enolase, esterases, fumarate hydratase, aspartate aminotransferase, glucose- ... | 2006 | 17547051 |
| dna quantities and qualities from various stages of some trematodes using optical and hat-rapd methods. | the aim of this experiment was to minimize dna quantity and quality for detection by optical (spectrophotometer at 260 nm and 280 nm) and hat-rapd methods. total dna from different stages, adult, metacercaria and eggs of 6 trematode species were isolated for analysis. in this experiment, the adult trematodes were classified into 3 groups by size: small, haplorchis taichui and stellantchasmus falcatus; medium, opisthorchis viverrini and ganeo tigrinus; and large, paramphistomum epiclitum and fisc ... | 2006 | 17547055 |
| epidemiology of food-borne trematodes and other parasite infections in a fishing community on the nam ngum reservoir, lao pdr. | food-borne trematodes (fbt) are important causes of parasitic infections in many asian countries. parasitological surveys in xai udom, a small fishing community on the nam ngum reservoir, lao people's democratic republic, revealed an overall parasitic infection rate in may 1999 of 68.8% (n = 173) and in december 1999 of 65.9% (n = 261). the liver fluke, opisthorchis viverrini accounted for most of the infections (prevalences of 53.8% and 42.1%, during the first and second surveys, respectively). ... | 2006 | 17333758 |
| field investigation of bithynia funiculata, intermediate host of opisthorchis viverrini in northern thailand. | a survey of freshwater snail, bithynia funiculata, was conducted in four locations, doi saket, mueang, saraphi and mae rim districts of chiang mai province, northern thailand, between june and october, during the rainy season of 2004. a total of 2,240 snails was collected and classified into 7 families and 15 genera; of which 352 b. funiculata were obtained. b. funiculata was found most abundant in july and september. the infection rate of trematode cercariae in b. funiculata was 9.6% (19/352), ... | 2006 | 17121291 |
| genetic markers for the identification and characterization of opisthorchis viverrini, a medically important food borne trematode in southeast asia. | the liver fluke, opisthorchis viverrini, is one of the major food borne trematodes in southeast asia, where infection causes hepatobiliary disease and subsequent development of cholangiocarcinoma. in thailand, o. viverrini is most prevalent in the northeast where there is marked regional variation in the rate of infection in humans at provincial, district and village levels. to date, the roles of genetic variation of o. viverrini on this observed variability in infection, transmission and associ ... | 2006 | 17166476 |
| ultrasound screening for opisthorchis viverrini-associated cholangiocarcinomas: experience in an endemic area. | in thailand, liver cancer is the most common malignancy in males and the third most common among females. in the northeast region, cholangiocarcinoma (chca) is the prevalent type, with opisthorchis viverrini (ov), an endemic liver fluke, being considered the cause. we evaluated the role of ultrasound (u/s) for cholangiocarcinoma screening as part of a larger cohort to characterize the linkage between liver fluke infestation and chca in khon kaen (northeast thailand). most people (77%) had normal ... | 2006 | 17059338 |
| effect of artesunate and artemether against clonorchis sinensis and opisthorchis viverrini in rodent models. | food-borne trematodiasis is an emerging public health problem and there is a need to develop novel treatment options. we examined the in vivo efficacy of single oral doses of artesunate and artemether administered to rodents experimentally infected with either clonorchis sinensis or opisthorchis viverrini. rats infected with adult c. sinensis were administered artesunate or artemether at doses of 75, 150 or 300 mg/kg. hamsters infected with adult o. viverrini were administered artesunate or arte ... | 2006 | 16973335 |
| comparison of gene expression profiles between opisthorchis viverrini and non-opisthorchis viverrini associated human intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is the second most common primary cancer in the liver, and its incidence is highest in the northeastern part of thailand. iccs in this region are known to be associated with infection with liver flukes, particularly opisthorchis viverrini (ov), as well as nitrosamines from food. to clarify molecular mechanisms of icc associated with or without liver flukes, we analyzed gene expression profiles of ov-associated iccs from 20 thai patients and compared their pr ... | 2006 | 17006947 |
| prevalence of blastocystis hominis infection in asymptomatic individuals from bangkok, thailand. | fresh stool examination was performed from 2,230 participants who enrolled in annual check-up programs of the faculty of medical technology, mahidol university in 1999-2000 and 2004. in this study, blastocystis hominis infection was diagnosed by culturing in jones' media. a total of 21% of fecal specimens (in 1999-2000) and 13.7% (in 2004) were positive for b. hominis. the vacuolated form was the predominant form found in culture solution after 48 hours of incubation. the distribution of infecti ... | 2005 | 16438173 |
| methylene blue staining method for identification of opisthorchis viverrini egg. | methylene blue staining method was used to distinguish o. viverrini eggs from haplorchis taichui and prosthodendrium molenkampi eggs. all eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. the distinct musk-melon-like texture of the o. viverini eggshell surface and the thread-like texture of h. taichui eggshell surface were recognized, while p. molenkampi eggs showed a smooth eggshell. we also evaluated the ... | 2005 | 16438191 |
| emergence of opisthorchis viverrini cercariae from naturally infected bithynia (digoniostoma) siamensis goniomphalos. | under natural conditions, the emergence of opisthorchis viverrini cercariae from naturally infected bithynia (digoniostoma) siamensis goniomphalos showed diurnal periodicity, peaking between 8:00-10:00 am. the cercariae did not emerge during darkness, but low-intensity light could induce a release. cercariae shedded from each field infected b.(d.) s. goniomphalos was recorded daily. the maximum output from one snail was 1,728 cercariae in a day. the total cercarial output from all five infected ... | 2005 | 16438207 |
| detection of opportunistic and non-opportunistic intestinal parasites and liver flukes in hiv-positive and hiv-negative subjects. | we assessed the frequency and distribution of infection with opportunistic and non-opportunistic intestinal parasites and the liver fluke, opisthorchis viverrini, in hiv-seropositive and hiv-seronegative subjects. age- and sex-matched hiv-seropositive (n = 78) and hiv-seronegative patients (n = 78) from two hospitals in khon kaen province, thailand, participated in this study from november 1998 to august 2000. these subjects were divided according to the presence of diarrhea and cd4 counts. a si ... | 2005 | 16295534 |
| detection of paragonimus heterotremus eggs in experimentally infected cats by a polymerase chain reaction-based method. | a polymerase chain reaction (pcr) procedure for the detection of paragonimus heterotremus eggs in stool samples was developed and compared with stoll's egg count method. the primers were designed on the basis of a previously constructed pph-13-specific dna probe, which produced an approximate 0.5-kb amplified product. this pcr method could detect as few as 5 eggs in 0.6 g of artificially inoculated feces of a healthy control cat or as little as 1 x 10(-4) ng of p. heterotremus genomic dna. the a ... | 2005 | 15856902 |
| opisthorchis viverrini antigen induces the expression of toll-like receptor 2 in macrophage raw cell line. | opisthorchis viverrini infection induces inflammation in and around the bile duct, leading to cholangiocarcinoma in humans. to examine the mechanism of o. viverrini-induced inflammatory response, we assessed the expression of toll-like receptors (tlrs) in raw 264.7 macrophage cell line treated with an extract of o. viverrini antigen. flow cytometry and immunocytochemistry showed that o. viverrini antigen induced the expression of tlr2 but not tlr4. western blotting and immunocytochemistry reveal ... | 2005 | 15862573 |
| prevalence of strongyloides in northern thailand and treatment with ivermectin vs albendazole. | the stools of 697 cases were examined by agar plate technique at tambon makam luang, sun pa tong district, chiang mai; there were strongyloides stercoralis 15.9%,opisthorchis viverrini 5.1%, intestinal fluke 0.1%. treatment with ivermectin 78 cases and albendazole 33 cases of strongyloidiasis gave cure rates at 98.7% and 78.7%, respectively. alkaline phosphatase in some patients were increased at mild level after treatment. side effects in ivermectin group were anorexia, nausia, diarrhea, diffus ... | 2005 | 15916052 |
| association of parasitic infections and cancers. | recent advances in the fields of molecular biology, epidemiology and infectious diseases have led to significant revelations to clarify the relationship between cancer and infective agents. this article reviews the relationship between parasitic infections and carcinogenesis and the possible mechanisms involved. few parasites, e.g., schistosoma haematobium and opisthorchis viverrini have been found to be strongly associated with bladder cancer and cholangiocarcinoma respectively. the evidence fo ... | 2005 | 15928434 |
| establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (kku-100). | to establish and characterize a new cholangiocarcinoma cell line from a patient living in the opisthorchis viverrini (o. viverrini) endemic area of northeast thailand. | 2005 | 15948244 |
| genetic and environmental determinants of risk for cholangiocarcinoma via opisthorchis viverrini in a densely infested area in nakhon phanom, northeast thailand. | infection with opisthorchis viverrini (ov) is associated with cholangiocarcinoma. ov is common in northeast thailand, but less than 10% of the inhabitants develop cholangiocarcinoma. animal experiments suggest that ov infection alone does not cause cholangiocarcinoma, and thus other environmental and genetic factors may play a role in causation. we conducted a population-based case-control study in which sex, age and place of residence were matched individually. polymorphisms of gstm1 and gstt1 ... | 2005 | 15957169 |
| freshwater mollusks of medical importance in kalasin province, northeast thailand. | a snail survey was performed in six districts around irrigation areas of lampao dam, in kalasin province. the survey caught a total of 5,479 live snails and classed them into five families, 12 genera and 15 species, of which 7 species are suspected of transmitting human parasitic diseases. the seven species were pila polita, pomacea canaliculata, filopaludina (s.) m. martensi, bithynia (digoniostoma) siamensis goniomphalos, melanoides tuberculata, radix rubiginosa, and indoplanorbis exustus. of ... | 2005 | 16124433 |
| application of dipstick dye immunoassay (ddia) kit for the diagnosis of schistosomiasis mekongi. | the dipstick dye immunoassay (ddia), developed in china for the detection of antibodies against schistosoma japonicum, relies on soluble egg antigen (sea) labelled with a colloidal dye. this assay is not only rapid, simple and inexpensive, but also particularly useful for screening in the field. in order to determine whether s. japonicum antigens are sufficiently cross-reactive to make the assay applicable for the diagnosis also of s. mekongi a ddia approach based on the s. japonicum sea was tri ... | 2005 | 16143289 |
| mixed infections with opisthorchis viverrini and intestinal flukes in residents of vientiane municipality and saravane province in laos. | faecal examinations for helminth eggs were performed on 1869 people from two riverside localities, vientiane municipality and saravane province, along the mekong river, laos. to obtain adult flukes, 42 people positive for small trematode eggs (opisthorchis viverrini, heterophyid, or lecithodendriid eggs) were treated with a 20-30 mg kg(-1) single dose of praziquantel and purged. diarrhoeic stools were then collected from 36 people (18 in each area) and searched for helminth parasites using stere ... | 2005 | 16153322 |
| a prospective study of diet, lifestyle, and genetic factors and the risk of cancer in khon kaen province, northeast thailand: description of the cohort. | cohort studies are the preferred design in observational epidemiology, but few involving the general population have been performed in asia, and most concern affluent urban populations. the khon kaen study has recruited about 25,000 subjects, aged mainly 35-64, from villages in the relatively underdeveloped north-east of thailand. all subjects underwent simple physical examination, completed an interviewer-administered questionnaire (including sections on lifestyle, habits, and diet) and donated ... | 2005 | 16235989 |
| repeated infection with opisthorchis viverrini induces accumulation of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanine in the bile duct of hamsters via inducible nitric oxide synthase. | chronic inflammation induced by repeated infection with opisthorchis viverrini has been postulated to be a risk factor for cholangiocarcinoma. to clarify the mechanism of carcinogenesis induced by repeated o.viverrini infection, we investigated the timecourse of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) formation, inducible nitric oxide synthase (inos) expression, nitric oxide production and pathological features in hamsters with two (2-if) or three (3-if) o.viverrini infe ... | 2004 | 15059927 |
| prevalence of opisthorchis viverrini infection and incidence of cholangiocarcinoma in khon kaen, northeast thailand. | liver cancer is the most common cancer in khon kaen, northeast thailand, because of the high incidence of cholangiocarcinoma (chca). opisthorchis viverrini (ov), a liver fluke, is endemic in the area, and has been evaluated as a cause of chca by international agency for research on cancer. residents of 20 districts in the province were invited to attend a mobile screening programme between 1990 and 2001. of 24 723 participants, 18 393 aged 35-69 years were tested for ov infection, by examining s ... | 2004 | 15117303 |
| epidemiology of liver cancer: an overview. | the incidence of liver cancer varies widely throughout the world, with high rates in sub - saharan africa, eastern and southeastern asia, and melanesia and a low incidence in northern and western europe and the americas. primary cancers of the liver in adults are of two main histological types: hepatocellular carcinoma, which is derived from hepatocytes, and cholangiocarcinoma, which is derived from the epithelial lining of the intrahepatic bile ducts. hepatocellular cancer is a frequently occur ... | 2004 | 15244512 |
| epidemiological survey of intestinal parasitic infections of diarrhoeal patients in nepal and lao pdr. | intestinal parasitological survey was conducted to clarify the distribution of intestinal parasites in nepal and lao peoples' democratic republic (lao pdr) from 2001 to 2003. a total of 401 diarrheal fecal specimens were collected from patients aged one to 75 years (250 from nepal and 151 from lao pdr). the specimens were examined using the formalin-ether sedimentation (fes) and sucrose centrifugal flotation (scf) techniques. nine species (3 nematoda, 1 cestoda, and 5 protozoa) of parasites were ... | 2004 | 15449645 |
| opisthorchiasis-associated biliary stones: light and scanning electron microscopic study. | biliary stones are frequently encountered in areas endemic for opisthorchiasis in thailand. the present study was to describe the prevalence and pathogenesis of these stones. | 2004 | 15484308 |
| hepatobiliary changes, antibody response, and alteration of liver enzymes in hamsters re-infected with opisthorchis viverrini. | we investigated pathological changes, antibody response, and liver enzymes in hamsters re-infected with opisthorchis viverrini. group 1 received a single dose of 50 metacercariae; groups 2 and 3 were first dosed with of 30 metacercariae and re-infected with 20 more once or twice at three month intervals. inflammation and liver cell necrosis were observed on 3d (day 3) for group 3 and 7d for group 2 in comparison with 21d for group 1. pathological changes included peri-ductal fibrosis, bile duct ... | 2004 | 15491546 |
| mechanism of no-mediated oxidative and nitrative dna damage in hamsters infected with opisthorchis viverrini: a model of inflammation-mediated carcinogenesis. | inflammation mediated by infection is an important factor causing carcinogenesis. opisthorchis viverrini (ov) infection is a risk factor of cholangiocarcinoma (chca), probably through chronic inflammation. formation of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg), and expression of inducible nitric oxide synthase (inos) and heme oxygenase-1 (ho-1) were assessed in the liver of hamsters infected with ov. we newly produced specific anti-8-nitroguanine antibody without cross-rea ... | 2004 | 15491850 |
| increased cell proliferation of mouse fibroblast nih-3t3 in vitro induced by excretory/secretory product(s) from opisthorchis viverrini. | infection by opisthorchis viverrini is a strong risk factor for cholangiocarcinoma. however, the mechanism by which the parasite is involved in carcinogenesis is not clear. in addition to the direct damage of the bile duct epithelium via direct contact with o. viverrini, the excretory/secretory (es) product(s) released from the parasites may play important roles in this process. we therefore investigated the responses of a fibroblast cell line, nih-3t3, to es product(s) released from o. viverrin ... | 2004 | 15521634 |
| the identification of a clonorchis sinensis gene encoding an antigenic egg protein. | the cdna library of clonorchis sinensis was screened for genes encoding antigenic proteins by using sera from clonorchiasis patients. a gene of 888 bp encoding a 28-kda protein (cs28) was cloned and found to contain a high percentage of glycine (20%), tyrosine (11%), and lysine (11%). the amino acid sequence of cs28 showed 60% homology with the vitelline b precursor protein of opisthorchis viverrini and of 33% homology with the vitelline b1 and b2 proteins of fasciola hepatica. a strong positive ... | 2004 | 15616856 |
| molecular cloning and characterization of a glutathione s-transferase encoding gene from opisthorchis viverrini. | an adult stage opisthorchis viverrini cdna library was constructed and screened for abundant transcripts. one of the isolated cdnas was found by sequence comparison to encode a glutathione s-transferase (gst) and was further analyzed for rna expression, encoded protein function, tissue distribution and cross-reactivity of the encoded protein with other trematode protein counterparts. the cdna has a size of 893 bp and encodes a gst of 213 amino acids length (ov28gst). the most closely-related gst ... | 2004 | 15783135 |
| changing patterns in intestinal parasitic infections among southeast asian laborers in taiwan. | in order to determine whether there are changing patterns in intestinal parasitic infections among foreign laborers in north taiwan, one fecal specimen was obtained from each of 1,569 laborers and examined by the formalin-ethyl acetate sedimentation technique. the prevalence of intestinal parasitic infections was 8.2%. females (11.7%) had a significantly higher prevalence than males (6.9%), although there was no significant difference between filipinos (7.7%), thais (8.3%) and indonesians (12.5% ... | 2004 | 14586642 |
| opisthorchis viverrini and opisthorchiasis: the 21st century review. | 2003 | 14611870 | |
| opisthorchis viverrini and opisthorchiasis: a historical review and future perspective. | opisthorchiasis is caused by the liver fluke, opisthorchis viverrini. the fluke afflicts approximately seven million inhabitants in northeastern and northern thailand. the fluke utilizes, respectively, freshwater snails and cyprinoid fish as its first and second intermediate hosts. man is the accidental definitive host who acquires infection through the consumption of improperly cooked fish. information pertaining to the history, the life-cycle, the epidemiology and transmission dynamics, and di ... | 2003 | 14611871 |
| taxonomy and biology of liver flukes. | opisthorchis viverrini is a human liver fluke endemic in thailand, lao pdr and cambodia. three subspecies of bithynia siamensis and 18 species of cyprinoid fish are susceptible first and second intermediate hosts, respectively. the differentiation between the adult worm of o. viverrini and that of the closely related species, opisthorchis felineus and clonorchis sinensis, is mainly based on the shape and position of the testes and the arrangement of the vitellaria. eggs of these flukes are morph ... | 2003 | 14611872 |
| epidemiology of opisthorchis viverrini. | opisthorchis viverrini is a food borne trematode, important because of the sheer numbers of people infected and its serious morbidities such as hepatobiliary diseases and cholangiocarcinoma (chca). although infections are identified throughout southeast asia, the epi-center is northeast thailand, where high prevalence coexists with a high incidence of chca. in this review, we present the basic population features and the factors influencing transmission between the different hosts. | 2003 | 14611873 |
| immunology and molecular biology of opisthorchis viverrini infection. | opisthorchiasis is the major public health problems in laos pdr and thailand. the disease becomes chronic and persists for many years, leading to hepatobiliary disease and cholangiocarcinoma. less severe manifestations include cholangitis, chronic cholecystitis and cholelithiasis. a significant degree of humoral and cell mediated immune responses to the parasite can be detected both in patients and animal models. the patients igg levels appear to correlate with gall bladder size and dysfunction ... | 2003 | 14611874 |
| pathobiology of opisthorchiasis: an update. | opisthorchis viverrini infection is associated with several hepatobiliary diseases including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis and cholelithiasis. pathological consequences of o. viverrini infection occur mainly in the liver, extrahepatic bile ducts, gall bladder and kidney. these pathologies have been described in both humans and experimental animals. moreover, both experimental and epidemiological evidence strongly implicate the liver fluke infection in the etiolog ... | 2003 | 14611875 |
| comparative proteomic analysis of juvenile and adult liver fluke, opisthorchis viverrini. | we used comparative two-dimensional gel electrophoresis to highlight proteins that are differentially expressed in the maturation stage of the parasite opisthorchis viverrini (ov). the proteins differentially expressed in the juvenile/adult forms of the parasite are thought to be important for survival and pathogenesis. we used a nonlinear gradient ph ranged 3-10 strips for isoelectric focusing to resolve soluble proteins from four different maturation periods of ov from 1 week juvenile to 4 wee ... | 2003 | 14611878 |
| histological analysis of gallbladder diseases in relation to opisthorchiasis in endemic areas of thailand. | chronic gallbladder disease frequently accompanies infection with the liver fluke, opisthorchis viverrini, in northeast thailand. however, the pathology and pathogenesis of the gallbladder disease have not been described. accordingly, gallbladder specimens from 187 consecutive patients who had undergone cholecystectomy at a referral hospital in an endemic area in thailand were histologically characterized in relation to o. viverrini infection. the infection was assessed by the presence of parasi ... | 2003 | 14611879 |
| helminth parasites of cats from the vientiane province, laos, as indicators of the occurrence of causative agents of human parasitoses. | a total of 55 domestic cats (felis catus f. domestica) and one wild (bengal) cat (prionaluirus bengalensis) from the vientiane province, central laos, were examined for helminth parasites with emphasis given to potential human parasites. the following species were found (parasites infective to man marked with an asterisk): opisthorchis viverrini, haplorchis pumilio, h. taichui, h. yokogawai, stellantchasmus falcatus (digenea); spirometra sp., dipylidium caninum, taenia taeniaeformis (cestoda); c ... | 2003 | 14710631 |
| opisthorchis viverrini and opisthorchiasis: the 21st century review. proceedings of the congress of opisthorchiasis and cholangiocarcinoma. may 28-30, 2002. khon kaen, thailand. | 2003 | 14763464 | |
| experimental investigation of opisthorchiasis-associated cholangiocarcinoma induction in the syrian hamster - pointers for control of the human disease. | appropriate animal models for specific diseases in man can facilitate elucidation of mechanisms underlying tumour development and allow potential interventions and therapeutic regimens to be tested in vivo before consideration for use in the human situation. in the north-east of thailand exceptionally high levels of cholangiocellular carcinomas (cccs) are encountered, related to infestation with opisthorchis viverrini liver flukes. the syrian hamster can also be infected with metacercariae of th ... | 2003 | 12875618 |
| 8-nitroguanine formation in the liver of hamsters infected with opisthorchis viverrini. | nucleic acid damage by reactive nitrogen and oxygen species may contribute to the carcinogenesis associated with chronic infection and inflammation. we examined 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) formation and nitric oxide (no) production in hamsters infected with opisthorchis viverrini (ov). formation of 8-nitroguanine was assessed immunohistochemically with an antibody specific for 8-nitroguanine. 8-nitroguanine formation was found mainly in the cytoplasm and slig ... | 2003 | 12963027 |
| specific and common antigens of clonorchis sinensis and opisthorchis viverrini(opisthorchidae, trematoda). | the antigenic characterizations and serological reactions of human liver flukes, clonorchis sinensis and opisthorchis viverrini, were analyzed by immunoblot. the antigenic profiles of the crude extract of clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kda, and those of opisthorchis 34-37, 43, 70, and 100 kda. of these, the 8, 26-28 and 34-37 kda bands of clonorchis and the 100 kda of opisthorchis were major components of each excretory-secretory antigen. the 8 and 26-28 kda b ... | 2003 | 12972729 |
| detection of opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes by a pcr-based method. | a pcr procedure for the detection of opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes was developed. this procedure was based on primers designed from a pov-a6 specific probe sequence giving a 330 base-pair product. the detection was accomplished in host tissue homogenates to which a single cercaria or metacercaria was introduced. pcr can detect as little as a single cercaria artificially inoculated in a snail or a single metacercaria artificially inoculated ... | 2003 | 12613764 |
| prevalence of intestinal parasite infections on a national scale among primary schoolchildren in laos. | in order to investigate the epidemiological situation of intestinal parasite infections in laos, parasitological surveys were carried out on a national scale including 17 provinces and the vientiane municipality. a total of 29,846 stool specimens were collected from primary schoolchildren from may 2000 to june 2002 and examined once with the cellophane thick smear technique. the cumulative egg positive rate for intestinal helminths was 61.9%. by species, the rate for ascaris lumbricoides was 34. ... | 2003 | 14574555 |
| [a case of severe eosinophilia in the acute phase of opisthorchis viverrini infection]. | a 26-year-old woman japanese public official servant in lao people's democratic republic was introduced to our hospital on december 12, 2002, because of two months duration of low grade fever and severe eosinophilia. there was no significant finding in physical examination. laboratory tests showed leukocytosis (15,700/microliter) with severe eosinophilia (42%), and no abnormal lymphocyte was observed. furthermore, elevation in alkaline phosphatase (748 iu/l), gamma-glutamyl transpeptidase (190 i ... | 2003 | 14574844 |
| epidemiology of strongyloides stercoralis in north-east thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay. | cross-sectional surveys of parasitic infection were performed using the agar plate culture technique (apct) and modified formalin-ethyl acetate concentration technique (mfect) to assess the true prevalence of strongyloides stercoralis relative to other parasites in north-east thailand. the enzyme-linked immunosorbent assay (elisa) for diagnosis of s. stercoralis infection was used to estimate the seroprevalence for comparison with coproprevalence. faecal and serum samples were collected from stu ... | 2003 | 15259466 |
| intestinal parasitic infections in hill-tribe schoolchildren in chiang mai, northern thailand. | we surveyed intestinal parasitic infections in hill-tribe schoolchildren residing permanently in chiang mai province. the positive rate, of 403 stool specimens examined using the formalin-ether sedimentation technique, was 48.9%. no significant difference between male (50.8%) and female (47.1%) students was found for the infection. the most common protozoa was entamoeba coli (40.9%), followed by giardia lamblia (14.9%). the most common helminth was hookworm (13.4%), followed by ascaris lumbricoi ... | 2003 | 19230577 |
| studies on concomitant antigens of bithynia funiculata for detection of antibody to opisthorchis viverrini: effect of different centrifugal speeds on antigen preparation. | antigens derived from somatic extracts of bithynia funiculata, an intermediate snail host of o. viverrini, have been demonstrated to be highly heterogeneous in molecular weight (mw). these antigens have been suggested to be of potential use for serodiagnosis. in this study, b. funiculata somatic antigens were extracted using five different centrifugal speeds, namely 10,000 (c1); 20,000 (c2); 30,000 (c3); 40,000 (c4) and 50,000 (c5) rpm, with the aim of removing some non-specific antigens and det ... | 2003 | 19230581 |
| motility of minute intestinal fluke, haplorchinae spp, metacercariae in fish dishes prepared by different uncooked methods. | fish-borne trematode is a worldwide problem, with the number of people infected by liver flukes alone estimated at 21 million. in addition to the major liver flukes, opisthorchis viverrini and clonorchis sinensis, several types of intestinal flukes also use cyprinoid fish as the intermediate host. traditional ingestion of undercooked and uncooked fish preparations is a major factor in pathogen acquisition. | 2002 | 11965210 |
| gall bladder and extrahepatic bile duct changes in opisthorchis viverrini-infected hamsters. | opisthorchis viverrini infection is associated with several hepatobiliary diseases, but few reports have described extrahepatic lesions in opisthorchiasis. we therefore sequentially investigated histological changes of the gall bladder and extrahepatic bile duct in hamsters infected with 25 (group 1), 50 (group 2) and 100 (group 3) metacercariae for up to 180 days. acute inflammatory reactions, including congestion, neutrophil and eosinophil infiltration, occurred in the gall bladder as early as ... | 2002 | 12062790 |
| liver fluke-associated cholangiocarcinoma. | infection with liver flukes has been reported to be associated with bile duct malignancy. | 2002 | 12153620 |
| prevalence of parasitic infections among thai patients at the king chulalongkorn memorial hospital, bangkok, thailand. | parasitic diseases are still considered to be a major public health problem. most patients with parasitic infections are asymptomatic and therefore remain undetected. asymptomatic parasitic infections are usually discovered by routine parasite examination. to determine the result of parasite examination at the parasitology unit, out patient department, king chulalongkorn memorial hospital, bangkok, thailand, the authors collected the data of individuals examined for parasite infections from june ... | 2002 | 12188445 |
| a cross-sectional study of intestinal parasitic infections among schoolchildren in nan province, northern thailand. | a cross-sectional study of the prevalence of intestinal parasitic infections at eight schools in bo klau district and four schools in chalerm prakiet district, nan province, in january and february, 2001. a total of 1,010 fecal samples were examined using the formalin-ether sedimentation technique. results revealed that the rate of helminthic infection was 60.0%, while protozoa accounted for 36.2% of infections; mixed infections were common, resulting in a total prevalence of both parasites of 6 ... | 2002 | 12236415 |
| serodiagnosis of human opisthorchiasis using cocktail and electroeluted bithynia snail antigens. | cocktail and electroeluted antigens from bithynia goniomphalos, the snail intermediate host of opisthorchis viverrini, were extracted and purified. the performance of these two antigens in the antibody detection of human opisthorchiasis was evaluated by indirect elisa. serum samples from people whose stool was either: (i). positive for opisthorchis eggs (n=61); or (ii). positive for at least one of 19 other species of parasite (n=125); or (iii). clear of parasites (n=30) were tested. the sensiti ... | 2002 | 12243778 |
| detection of opisthorchis viverrini in human stool specimens by pcr. | 2002 | 12354909 | |
| expression of tenascin in bile duct cancer of hamster liver by combined treatment of dimethylnitrosamine with opisthorchis viverrini infections. | tenascin is an extracellular matrix glycoprotein known to be an essential factor for the modulation of reciprocal interactions between the epithelium and mesenchyme during embryogenesis and tumourigenesis. the interactions between the expression of tenascin in the liver of syrian golden hamster and the development of bile duct cancer in an opisthorchis viverrini-associated cholangiocarcinoma model were investigated. the tenascin was expressed in connective tissues surrounding the dilated ducts, ... | 2002 | 12363380 |
| rifampin markedly decreases plasma concentrations of praziquantel in healthy volunteers. | praziquantel is extensively metabolized by the hepatic cytochrome p450 (cyp) enzymes. the cyp3a isoforms are likely to be major enzymes responsible for praziquantel metabolism. rifampin (inn, rifampicin), a potent enzyme inducer of cyp-mediated metabolism (especially cyp2c9, cyp2c19, and cyp3a4), is known to markedly decrease plasma concentrations and effects of a number coadministered drugs. the aim of this investigation was to study the possible pharmacokinetic interaction between rifampin and ... | 2002 | 12426514 |
| high prevalence of fasciolopsis buski in an endemic area of liver fluke infection in thailand. | there is a high prevalence of fasciolopsis buski in sawasdee village in the nam som district, udonthani province in northeastern thailand, an endemic area of liver fluke infection. from stool examination of 183 villagers participating in the study, the authors detected a fluke parasitic infection rate of 14.8% (27 cases). of interest, the authors found a similar high prevalence of both opisthorchis viverrini (16 cases, 8.7%) and f buski (13 cases, 7.1%). this region is the endemic area of liver ... | 2002 | 12466749 |
| a survey of metacercariae in cyprinoid fish in nakhon ratchasima, northeast thailand. | metacercariae were recovered from freshwater fish taken from a stream in lum cha muak, nakhon ratchasima province, northeast thailand during the year 2000. seventy-nine cyprinoid fish comprising 3 species (52 puntius leiacanthus, 22 cyclocheilichthys armatus, and 5 hampla dispar) were collected. the prevalence of infection with metacercariae was 51.9%. only two genera of metacercariae were found: haplochinae spp and heterophyidae spp. haplochinae metacercariae were found in all 3 species of fish ... | 2002 | 12971487 |
| a survey of trematode cercariae in bithynia goniomphalos in northeast thailand. | a survey of trematode larvae in the fresh water snail, bithynia goniomphalos in the main water reservoir of huai thalaeng district, nakhon ratchasima province, thailand, was conducted. the study site is in an area with a high prevalence of opisthorchis viverrini infection. malacological samplings were made during the year 2000 to study the prevalence and intensity of infection. parasitological examinations of 527 live snails were performed at the veterinary parasitology laboratory, chulalongkorn ... | 2002 | 12971488 |
| parasitic infections in thai workers that pursue overseas employment: the need for a screening program. | stool examination is a requirement of the screening program for thai workers seeking overseas employment. we report on the prevalence of intestinal parasitic infections among these workers; the stool examination results of 2,213 thai workers who visited the out-patients department of the king chulalongkorn memorial hospital between september 2000 and january 2001 were analysed. one hundred and thirty-five (6.1%) were found to be infected with 1 or 2 species of parasite. there were 54 (40%), 47 ( ... | 2002 | 12971489 |
| affinity purified oval antigen for diagnosis of opisthorchiasis viverrini. | monoclonal antibodies (mab) were raised against an oval antigen of the liver fluke opisthorchis viverrini which is the causative agent of a parasitosis, i.e. opisthorchiasis in thailand. the antibodies were used in an affinity column to purify the o. viverrini oval antigen from a crude extract of adult parasites by chromatography. the oval antigen was then used in a membrane (dot) elisa for detecting antibodies in serum samples of parasitologically confirmed opisthorchis viverrini infected indiv ... | 2001 | 12009074 |
| diagnosis of intestinal amebiasis using salivary iga antibody detection. | attempts were made to use soluble antigen extract of strain hk-9 of entamoeba histolytica to detect salivary iga antibodies in intestinal amebiasis patients by using elisa. total salivary samples of 109 individuals were divided into four groups. group i comprised 32 patients whose stools were positive only for e. histolytica cysts and/or trophozoites. group ii comprised 12 individuals whose stools were positive for e. histolytica and other intestinal parasites. group iii comprised 36 individuals ... | 2001 | 12041582 |
| nucleotide sequence of mitochondrial co i and ribosomal its ii genes of opisthorchis viverrini in northeast thailand. | the mitochondrial cytochrome c oxidase subunit i (co i) gene and the second internal transcribed spacer region (its ii) gene of opisthorchis viverrini were compared among o. viverrini from various areas in northeast thailand. the nucleotide sequences of partial co i gene (417 bp) of o. viverrini differed among o. viverrini originated from ubon ratana, leongpleuy, ban phai, maha sarakham, and chatturat. these intraspecific variations were classified into 5 patterns but no area-specific pattem was ... | 2001 | 12041584 |
| prevalence of opisthorchis viverrini infection among villagers harboring opisthorchis-like eggs. | the precise occurrence of opisthochis viverrini infection in humans, who were positive for opisthorchis-like eggs in a stool examination, was determined using the potassium permanganate staining method. in the 68 specimens examined, there were more individual o. viverrini eggs (38.24%) than singular haplorchis taichui eggs (29.41%). one-fourth of the total specimens contained a mixed infection of o. viverrini and h. taichi eggs. the median ratio of o. viverrini: h. taichui eggs in mixed infectio ... | 2001 | 12041596 |
| the present situation of paragonimiasis in endemic area in phitsanulok province. | a cross-sectional survey and evaluation of paragonimiasis situation from endemic area in phitsanulok province was studied. studies on the species and prevalence of parasites which infected people in noen maprang, phitsanulok province were also conducted during october 1999-march 2000. the sputum specimens were collected and examined to identify paragonimus heterotremus eggs. in addition fecal samples were collected and examined for parasites by the formalin-ethyl acetate concentration technique. ... | 2001 | 12041605 |
| epidemiology of liver cancer in thailand. | the cancer registry is an essential part of any rational programme of cancer control. the information is the primary resource for epidemiological research and for planning and evaluating health services for the prevention, diagnosis and treatment of cancer. epidemiological research, based on comprehensive cancer registration, remains the most valid and efficient way to plan and evaluate all aspects of cancer control. the estimated incidence of primary liver cancer in thailand is very high, liver ... | 2001 | 12718642 |