Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| biosynthesis of riboflavin. an aliphatic intermediate in the formation of 6,7-dimethyl-8-ribityllumazine from pentose phosphate. | 6,7-dimethyl-8-ribityllumazine synthase deficient mutants of candida guilliermondii were divided into two groups on the basis of in vitro complementation. mutants of complementation group i produce an intermediate x from ribose 5-phosphate in a reaction requiring mg++ ions. compound x was partially purified and was shown to be a phosphoric acid ester. 6,7-dimethyl-8-ribityllumazine can be formed from compound x by cell extracts from mutants of complementation group ii. the reaction requires 5-am ... | 1985 | 3838473 |
| [genetic control of riboflavin biosynthesis in pichia guilliermondii yeasts. the detection of a new regulator gene rib81]. | the properties of mutants resistant to 7-methyl-8-trifluoromethyl-10-(1'-d-ribityl)-isoalloxazine (mtry) were studied. the mutants were isolated from a genetic line of pichia guilliermondii. several of them were riboflavin overproducers and had derepressed flavinogenesis enzymes (gtp cyclohydrolase, 6.7-dimethyl-8-ribityllumazine synthase) in iron-rich medium. an additional derepression of these enzymes as well as derepression of riboflavin synthase occurred in iron-deficient medium. the charact ... | 1985 | 3838729 |
| evaluation of the new mycotube test-kit for yeast identification. | the performance of a modified mycotube test for the identification of yeasts was evaluated using standard biochemical tests as reference. one hundred and eighty strains belonging to 12 medically important species (15 strains each) were tested. the overall rate of identification was 72%, and rose to 87.8% when macroscopic and microscopic morphologic features were also considered; a 100% rate was obtained with candida albicans, candida krusei, candida parapsilosis, candida pseudotropicalis, candid ... | 1985 | 3886377 |
| transformation of candida maltosa and pichia guilliermondii by a plasmid containing saccharomyces cerevisiae arg4 dna. | saccharomyces cerevisiae, candida maltosa and pichia guilliermondii have been transformed by the plasmid pye(arg4)411, which contains the s. cerevisiae arg4 gene inserted into pbr322. in all transformants argininosuccinate lyase as well as beta-lactamase were detected. the arg+ phenotype of transformants is mitotically unstable. closed circular pye(arg4)411 dna was detected in transformant dna preparations by hybridization to pbr322 dna and by transformation of e. coli to ampicillin resistance. | 1985 | 3916721 |
| [joint cultivation of streptococcus lactis, lmu strain, and various species of yeasts]. | when s. lactis, strain lmu and yeast were plated out simultaneously on media containing different amounts of amine nitrogen and vitamins, the yeast did not stimulate the synthesis of nisin. plating out of s. lactis on media with grown cultures of the rostov baker's yeast, american baker's yeast, candida guilliermondii, candida clausenii, fabospora fragilis, fabospora macedoniensis, octosporomyces octosporus, saccharomyces cerevisiae, races "ya" and "m" and saccharomyces globosus resulted in inhi ... | 1985 | 3923918 |
| [effect of 2-amino-2-thiazoline on the sulfhydryl group content of yeasts of various degrees of radiosensitivity]. | the influence of 2-amino-2-thiazoline on the level of total sulphydryl groups in the haploid and diploid yeast cells saccharomyces cerevisiae and pichia guilliermondii has been studied. the increase of radioresistance of the yeast cells has been shown to be connected with the growing content of the endogenous thiols. | 1984 | 6375739 |
| [studies on candida guilliermondii for the purpose of obtaining the maximum number of protoplasts]. | 1984 | 6377832 | |
| [alcohol oxidation by candida guilliermondii yeasts grown on hexadecanol]. | a number of enzyme systems involved in the first steps of hexadecane oxidation can be induced by hexadecanol, an intermediate product of hexadecane degradation. it has also been found that, in candida guilliermondii cells and in their mitochondrial fraction, an oxidase system is induced when the cells are grown on hexadecanol. this system is similar to that in cells grown on hexadecane; it oxidises higher alcohols at a high rate and is not inhibited by the inhibitors of the man phosphorylating r ... | 1984 | 6384744 |
| [the role of glutathione in natural and modified radioresistance of yeast cells]. | a study was made of the dependence of different natural and modified radioresistance upon glutathione content of yeast cells (saccharomyces cerevisiae and pichia guilliermondii). it was shown that glutathione was only involved in the formation of natural radioresistance in saccharomyces cerevisiae cells. it was also shown that the increase in the radioresistance of yeast cells under the effect of 2-amino-2-thiazoline was accompanied by the increase in the level of total glutathione in them. | 1984 | 6390498 |
| mycoflora of the human dermal surfaces. | the mycotic flora of the scalp and interdigital areas of the hand and foot of 1296 apparently healthy human inhabitants of three amazonian communities were surveyed by means of microscopic examination of epidermal scrapings and cultural isolation on mycosel agar. no macroscopic or microscopic evidence of fungal infection was detected in any of our study subjects. from 133 (10%) individuals, 143 fungi representing 13 genera and 39 species were recovered. yeasts constituted 85% of the fungi. seven ... | 1984 | 6509388 |
| biosynthesis of riboflavin. enzymatic formation of the xylene moiety from [14c]ribulose 5-phosphate. | we have studied the enzymatic formation of the xylene ring of riboflavin using cell extracts from the flavinogenic yeast candida guilliermondii. 5-amino-6-ribitylamino-2,4(1h,3h)-pyrimidinedione or its 5'-phosphate could serve as substrates. in addition, a pentose phosphate or pentulose phosphate was required. experiments with [14c]ribulose 5-phosphate gave evidence for the incorporation of the ribulose carbon atoms except c-4 into the xylene ring of the vitamin. | 1984 | 6546684 |
| defective phagocytosis in insulin controlled diabetics: evidence for a reaction between glucose and opsonising proteins. | neutrophils from diabetic patients controlled with insulin showed impaired phagocytosis of a yeast, candida guilliermondii. the defect was detected by measuring the initial rate of phagocytosis at optimal concentrations of phagocytes and organisms in autologous plasma. by mixing normal neutrophils in diabetic plasma and vice versa both cellular and plasma abnormalities were shown. the defect was reproduced by incubating normal plasma at a d-glucose concentration of 15 mmol/l for 3 h or for short ... | 1984 | 6747013 |
| amphotericin b tolerance: a characteristic of candida parapsilosis not shared by other candida species. | thirty yeast isolates from clinical specimens were tested for their susceptibility to amphotericin b at 30 c, 37 c, and 39 c. of the six candida albicans, five candida tropicalis, one candida guilliermondii, one candida krusei, one candida pseudotropicalis, two torulopsis glabrata, and four cryptococcus neoformans isolates tested, all were inhibited at amphotericin b concentrations of less than or equal to 0.4 micrograms/ml and killed by concentrations of amphotericin b that were less than or eq ... | 1983 | 6822746 |
| inhibition of complement-dependent phagocytosis by monocytes following pre-incubation with immune complexes and interaction with plasma. | normal monocytes have been pre-incubated with soluble immune complexes (ic) (bsa-anti-bsa) or heat-aggregated human igg (ahg) (ics) at pathophysiological concentrations. if these 'ic-monocytes' are allowed to interact with plasma they show reduced phagocytosis of candida guilliermondii targets. under the pre-incubation conditions used, 2% of offered ics remain cell associated and their binding to monocytes is energy-independent and complement-independent. at these low concentrations of ics, only ... | 1983 | 6831773 |
| comparison of the effects of filtration leucapheresis and discontinuous flow centrifugation leucapheresis on granulocyte microbicidal function. | in an investigation of the in vitro phagocytic and microbicidal function of granulocytes collected by filtration leucapheresis (fl) from 18 donors and by discontinuous flow centrifugation leucapheresis (dfc) from six donors, comparison was made with the function of granulocytes obtained from the same donors by venepuncture and density gradient centrifugation over ficoll-isopaque (fi). no significant impairment of the phagocytosis or killing of candida guilliermondii by either fl- or dfc-granuloc ... | 1983 | 6841650 |
| [purification and properties of gtp-cyclohydrolase of the yeast pichia guilliermondii]. | gtp-cyclohydrolase was isolated from the fe-deficient cells of pichia guilliermondii and purified 440-fold by treatment of extracts with streptomycin sulfate as well as by protein fractionation with (nh4)2so4 at 25-45% saturation, gel filtration through sephadex g-200 and deae-cellulose chromatography. the curves for the dependence of specific activity of gtp-cyclohydrolase on substrate and cofactor concentrations are non-hyperbolic; the values of [s]0.5 for gtp and mg2+ are 2.2 x 10(-5) and 2 x ... | 1983 | 6871289 |
| selective interaction of a fucus vesiculosus lectin-like mucopolysaccharide with several candida species. | a complex carbohydrate specific lectin-like mucopolysaccharide extracted from the brown alga fucus vesiculosus was found to agglutinate candida guilliermondii cells but not those of other species of the same genus (except for a weak agglutination with c. krusei). the selective binding of this mucopolysaccharide correspondingly affected the growth of the yeasts. the results suggest that there are notable differences--at least with respect to the receptors for the f. vesiculosus mucopolysaccharide ... | 1983 | 6223551 |
| defective neutrophil function and microbicidal mechanisms in the myelodysplastic disorders. | neutrophil function studies have been carried out in a series of 44 patients with primary myelodysplastic syndromes (mds). in vitro tests of phagocytosis and killing of candida guilliermondii and staphylococcus aureus identified 13 patients with abnormal neutrophil function at presentation and a further 10 who developed abnormalities during the course of their disease. the incidence of defective function in the five disease categories in this series was: refractory cytopenia (rc) 8/17; refractor ... | 1983 | 6311878 |
| [effect of iron, actinomycin d and cycloheximide on the gtp-cyclohydrolase synthesis in flavinogenic yeasts]. | the effect of fe on the gtp-cyclohydrolase activity of the yeasts pichia guilliermondii atcc 9058 and torulopsis candida bkm 13 whose flavinogenesis is controlled by fe was investigated. the gtp-cyclohydrolase activity of yeast cells grown in an iron-deficient medium was 40-50 times that of the cells grown in an iron-rich medium. in the latter case the incubation of cells with alpha, alpha'-dipyridyl or 8-oxyquinoline also increased the enzyme activity. cycloheximide prevented the rise in the cy ... | 1982 | 7199939 |
| [selection and mutant properties of pichia guilliermondii yeasts with derepressed gtp cyclohydrolase, the enzyme of the 1st step in flavinogenesis]. | a positive method is proposed for selecting pichia guilliermondii mutants with derepressed gtp cyclohydrolase. mutants with the incompletely blocked gene rib2 were used as parent strains; these can grow in a medium without riboflavin (rf) only if the enzyme is derepressed as the result of iron deficiency in cells. strains growing in a medium without rf at the optimal supply of cells with iron were selected as regulatory mutants. the mutants accumulated 6,7-dimethylpterin in high concentrations a ... | 1982 | 6280023 |
| phagocytosing neutrophils rapidly release a factor which inhibits granulopoiesis in vitro. | phagocytosis of the heat-killed opsonised yeast, candida guilliermondii, by human neutrophils resulted in the rapid release of a potent factor which suppressed granulopoiesis in vitro. the factor has been shown to act on monocytes and macrophages by inhibiting the production and release of colony-stimulating factor (csf) which is the specific stimulator of granulocytic colony-forming cell (cfuc) proliferation in vitro. when added directly to target cells containing cfuc, the inhibitory factor ha ... | 1982 | 6800198 |
| antibacterial activities of candida yeasts. partial purification and characterization of the active substance of candida guilliermondii. | the activity spectra of 25 candida strains on strains of different species of bacteria were recorded. the active substance produced by candida guilliermondii strain 848 was partially purified by cation exchange and chromatography gel filtration on sephadex g-15. the substance is heat stable (80 degrees c, 10 min), not susceptible to treatment with proteases and most probably of a molecular weight smaller than 3500 dalton. at a concentration of 50 a.u./ml it acts bacteriostatically on a staphyloc ... | 1982 | 6820590 |
| [fungal infections in urologic diseases with special reference to mixed bacterial infection]. | a report is given on the mycological examination of urine from 146 urological patients. the possible influence of a mixed infection with bacteria on the growth of blastomyces is discussed. whereas in the organism bacteria and blastomyces can exist well in conjunction, especially gram-negative bacteria in a culture medium can inhibit the growth of blastomyces. the result would be wrongly negative culture results. in our tests mixed infections of candida albicans and/or candida guilliermondii with ... | 1982 | 7048800 |
| [biosynthesis of 6,7-dimethyl-8-ribityllumazine in yeast extracts of pichia guilliermondii]. | the effects of some hexoses, pentoses, their phosphoric esters, ribitol and pyruvate on the synthesis of 6.7-dimethyl-8-ribityllumazine in extracts of prototrophic strain and riboflavin-deficient mutants of the yeast pichia guilliermondii atcc 9058 were studied. incubation of dialyzed cell extracts of the mutant strain rg123 (rib7, his-) with blocked riboflavin syntase in the presence of 2.5-diamino-6-hydroxy-4-ribosylaminopyrimidine-5'-phosphate, nadph and ribose-5-phosphate results in producti ... | 1982 | 7115806 |
| performance of yeast identification systems. an analysis of the college of american pathologists special mycology survey data. | the performance of approximately 400 participants in the cap special mycology survey in identifying 15 medically important yeasts was analyzed. with nine strains, users of the api 20c system and uni-yeast-tek (uyt) systems performed comparably. with two strains (trichosporon cutaneum and candida guilliermondii) the uyt users performed significantly better than api users; with four strains (cryptococcus albidus, saccharomyces cerevisiae, and two strains of candida tropicalis), api users significa ... | 1982 | 7137107 |
| [effect of serotonin of the survival of candida guilliermondii cells irradiated by short-wave ultraviolet light]. | 1982 | 7142566 | |
| [effect of riboflavin analogs on pichia guilliermondii growth]. | the work was aimed at studying the biological activity of 32 structural riboflavin (rf) analogs substituted at positions 7, 8 and 10 as well as with a modified structure of the isoalloxazine cycle and the side d-ribityl chain. an rf-dependent mutant of the yeast pichia guilliermondii ms1 was used as a test organism. the strain could grow in a medium without the vitamin in the presence of 2-thio-rf and analogs with esterified hydroxyls in the side chain, viz. tetraacetate and tetrabutyrate of rf. ... | 1982 | 7144611 |
| [aromatic hydrocarbons as enzyme inducers of cleavage of the aromatic ring in candida guilliermondii]. | monocyclic and dicyclic aromatic hydrocarbons, toluene and 2-methylnaphthalene, can induce pyrocatechate-1,2-oxygenase in candida guilliermondii bkm y-916 cells. the enzyme is active at ph 7.0. the culture oxidizes toluene via benzoic acid by ortho-cleavage. the results indicate that candida as an ecologo-trophic group of microorganisms can be involved, along with bacteria, in biodegradation of various aromatic hydrocarbons. | 1982 | 7155001 |
| [arthrobacter globiformis - a new yeast-lysing bacterium]. | a bacterium capable to lyse viable yeast cells was isolated from compost enriched with baker's yeast cells and was identified as arthrobacter globiformis. the yeast lytic enzyme complex produced by shaking culture was precipitated with ammonium sulfate, dialyzed and lyophilized. the crude residue contained beta-glucanase and alpha-mannanase, yet not chitinase. optimale carbon sources in the culture medium for a high enzyme synthesis were 0.5% beta-glucan for the production of beta-glucanase, res ... | 1982 | 7157841 |
| [use of a candida guilliermondii allergen for diagnostic purposes]. | 1982 | 7170743 | |
| [method of identification of industrial strains of candida guilliermondii]. | 1982 | 6177915 | |
| [purification and properties of pichia guilliermondii yeast alkaline nucleotide pyrophosphatase hydrolyzing flavin adenine dinucleotide]. | alkaline nucleotide pyrophosphatase was isolated from the pichia guilliermondii wickerham atcc 9058 cell-free extracts. the enzyme was 740-fold purified by saturation of ammonium sulphate, gel-chromatography on sephadex g-150 and ion-exchange chromatography on deae-cellulose. nucleotide pyrophosphatase is the most active at ph 8.3 and 49 degrees c. the enzyme catalyzes the hydrolysis of fad, nad+, nadh, nadph, gtp. the km value for fad is 2.4 x 10(-4) m and for nad+--5.7 x 10(-6) m. the hydrolys ... | 1981 | 6111146 |
| [conditions and dynamics of citrate, isocitrate and polyol excretion in yeast growth on glucose]. | the dynamics of metabolite excretions by candida guilliermondii, candida zeylanoides and torulopsis candida was studied during their batch cultivation in media with glucose excess and low concentrations of n, p or s sources; the latter were the growth limiting factors and determined the transition to phase of retardation and the stationary phase of growth. growth limitation with the above components in the presence of a carbon source results in the following: (a) the growth becomes linear and th ... | 1981 | 7198180 |
| involvement of near-uv-induced synthesis of serotonin in photoprotection and in potentiation of far-uv lethality in the yeast candida guilliermondii. | 1981 | 7280040 | |
| a survey of fungi and some indicator bacteria in chlorinated water of indoor public swimming pools. | fifty-four water samples, of volume 500 ml, originating from six public indoor fresh water swimming pools were examined for the presence of fungi and some indicator bacteria by a membrane-filter method. sabouraud-dextrose agar and selective candida albicans-medium were used for isolation and identification of fungi. in all but one of the samples the free chlorine content was above 0.40 mg/l. no candida albicans were detected. molds and unidentified yeasts were isolated from 29 of the samples. th ... | 1981 | 7304014 |
| [riboflavin transport in mutant of pichia guilliermondii yeast with damaged glucose uptake]. | the mutant of p. guilliermondii yeast ms-50 which had lost the ability to grow in a glucose-containing medium but not in the presence of sucrose or maltose was selected. the mutant has a damaged glucose uptake from the medium. the riboflavin permease activity of the ms1-50 mutant is not impaired; glucose inhibits the riboflavin uptake by this strain. thus, riboflavin permease does not promote glucose transport into yeast cells. | 1981 | 7306594 |
| biosynthesis of riboflavin. characterization of the product of the deaminase. | the 2'5-diamino-6-ribitylamino-4(3h)-pyrimidinone 5'-phosphate deaminase was partially purified from cell extracts of candida guilliermondii atcc 9058. the enzyme requires mg2+ for activity. maximal activity was observed at ph 7,3. the enzyme converts its substrate, 2,5-diamino-6-ribitylamino-4(3h)-pyrimidinone 5'-phosphate, to 2,5-diamino-6-ribitylamino-2,4(1h,3h)-pyrimidinedione 5'-phosphate. this labile compound was treated with diacetyl and the resulting 6,7-dimethyl-8-ribityllumazine 5'-pho ... | 1981 | 7317443 |
| [activity of the enzyme of the 2d step of flavinogenesis, 2,5-diamino-6-hydroxy-4-ribosylaminopyrimidine-5'-phosphate reductase, in pichia guilliermondii yeasts]. | the activity of 2,5-diamino-6-hydroxy-4-ribosylaminopyrimidine-5'-phosphate reductase, an enzyme of the second step of flavinogenesis, was studied in the yeast pichia guilliermondii using a new fluorimetric technique. the activity of the enzyme was found in extracts of the wild-type strain and the genotype rib mutants but not in extracts of the fm 1-4 (rib 2) mutant. interallelic complementation was revealed in the gene rib 2. the activity of the enzyme was identical in extracts of the cells gro ... | 1981 | 7329350 |
| bovine porphyria caused by candida guilliermondii infection. | 1981 | 7342738 | |
| [aggregation and separability of the shikimate pathway enzymes in yeasts]. | gel filtration was employed to estimate the molecular weights and to determine possible physical aggregation of enzymes [5-dehydroquinate synthase (dhq synthase), 5-dehydroquinase (dhqase, ec 4.2.1.10), shikimate: nadp oxidoreductase (ec 1.1.1.25), shikimate kinase (ec 2.7.1.71), 3-enolpyruvylshikimate 5-phosphate synthase (epsp synthase)] in the shikimate pathway in eleven species of yeasts. the five enzymes were not aggregated in extracts of hansenula henricii, h. fabianii, h. anomala, candida ... | 1981 | 6270920 |
| [role of endogenous substances in creating a background of increased radioresistance. 15. serotonin participation in the formation of radioresistance in the photoprotection of pichia guilliermondii yeast cells]. | 1981 | 6458063 | |
| [increase in yeast and bacterial sensitivity to inhibitors and riboflavin as affected by high sulfate and phosphate concentrations]. | cultivation of the yeast pichia guilliermondii in a medium with a high content of sulfate or phosphate ions (0.6 m and higher) increased its susceptibility to actinomycin d and 7-methyl-8-trifluoromethyl 10-(1'-d-ribityl)isoalloxazin, and analog of riboflavin, and decreased the requirement of the riboflavin-dependent mutant p7 in exogenous vitamin b2. the protoplasts of the yeast were also very susceptible to actinomycin d when they were incubated in a medium with a high sulfate concentration. s ... | 1981 | 7017354 |
| in vitro susceptibility to 5-fluorocytosine and nystatin of common clinical yeast isolates. | the sensitivity to nystatin, 5-fluorocytosine (5-fc) or both was studied for 131 clinical isolates of candida albicans, 47 of candida parapsilosis, 34 of candida tropicalis, 7 of candida guilliermondii, 28 of torulopsis glabrata and 1 of torulopsis candida. all strains were inhibited by concentrations of nystatin within the usual range of sensitivity except one strain of t. glabrata and another of t. candida whose minima inhibitory concentrations (mics) were respectively 250 u/ml and greater tha ... | 1981 | 7012631 |
| [cell electrophoretical characterization of the surface of candida guilliermondii]. | the electrical properties of the outer layer of the cell wall of a hydrocarbon-grown yeast c. guilliermondii were studied in detail by cell electrophoresis. some experiments were also made with the yeast s. cerevisiae. the mobilities of the yeasts were measured as a function of ph at ph 2-11.5 and constant ionic strength i = 0.02 mol/l. for identification of surface groups the ph-mobility curves are used to calculate pk-values. the results indicate the presence of amino and carboxyl groups on th ... | 1980 | 7013285 |
| [intrinsic luminescence of pichia guilliermondii in the growth process]. | intrinsic luminescence of pichia guilliermondii cells was registered during the whole cycle of their growth on the solid medium using a device for measuring superweak luminescence equipped with a photomultiplier sensitive in the uv region (quantum yield about 25%), provided the temperature of the object and that of pm were equal. | 1980 | 7207255 |
| [detection of phosphorylated pyrimidine precursors of riboflavin in yeasts]. | the nature of products formed from gtp in the presence of nadph under effects of dialyzed extracts of the prototrophic strain of pichia guilliermondii atcc 9058 and mutants rib2 and rib3 with blocked second and third steps of flavinogenesis, respectively, was studied. the unstable pyrimidine intermediates were identified on the basis of the nature of pteridines formed upon condensation of riboflavin precursors with diacetyl. it was shown that under the action of extracts of the mutants rib3 gtp ... | 1980 | 7213861 |
| impaired neutrophil function and myeloperoxidase deficiency in pregnancy. | the ability of neutrophils to phagocytose and kill candida guilliermondii was investigated in 28 pregnant women and compared wtih 29 healthy controls. phagocytosis was normal, but the ability to kill and digest candida was reduced to 65% of normal. quantitative myeloperoxidase measurement revealed a considerable reduction of this enzyme (55% of normal) while neutrophil alkaline phosphatase levels were nearly twice as high as in normals. it is suggested that both lack of myeloperoxidase and impai ... | 1980 | 6246914 |
| [changes in the membrane atpase activity of erythrocytes and candida guilliermondii under the action of roseofungin]. | changes in the mg-atpase and na, k-atpase activity of the rat erythrocyte and candida guilliermondii membranes under the effect of roseofungin were studied. the antibiotic was totally bound to the isolated plasmatic membranes of candida guilliermondii, up to 3 micrograms of the antibiotic per 1 microgram of the yeast protein. the mg-aptase activity of these membranes was slightly inhibited by the antibiotic. the activity of na, k-atpase was almost completely inhibited even at 0.04 mg of roseofun ... | 1980 | 6255858 |
| [comparative study of the radioprotective effectiveness of sulfur-containing radioprotective agents in experiments on saccharomyces cerevisiae and pichia guilliermondii]. | 1980 | 6996030 | |
| [cytochrome p-450 content in yeast cells during growth on hexadecane]. | the content of cytochrome p-450 was studied in the cells of alkane oxidizing yeasts candida guilliermondii, c. tropicalis and c. lipolytica. the cells of all the studied yeast strains growing on hexadecane were found to contain cytochrome p-450. the cytochrome was not detected when the yeast strains grew on glucose. the concentration of cytochrome p-450 remained constant at the exponential growth phase, but decreased at the beginning of the stationary growth phase. cytochrome p-450 was shown to ... | 1980 | 6997703 |
| riboflavin accumulation by cells of the yeast pichia (candida) guilliermondii. | 1980 | 6999753 | |
| biosynthesis of riboflavin in vitro. isotopic incorporation studies in pichia guilliermondii extracts. | in the riboflavin-deficient mutant rib3 and in wild-type cells of p. guilliermondii, gtp is transformed into 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine and riboflavin, respectively. we were able to demonstrate the partial in vitro synthesis of these compounds, including a reductive conversion step of the product of gtp cyclohydrolase ii action upon labelled [14c]gtp. in order to analyse the pyrimidine derivatives formed, 6,7-dimethyl-8-ribitylpterin and riboflavin were synthesized by additio ... | 1980 | 7357033 |
| [production and genetic analysis of pichia guilliermondii wicherham mutants that do not assimilate hexadecane]. | 1980 | 7382863 | |
| [assimilation of n-alkanes with a varying length of the carbon chain by the yeast candida guilliermondii]. | using radioactive tracers, the effect of n-alkanes with a varying length--c16-c25--of the carbon chain on the rate of yeast growth and the ratio of major chemicals in their biomass was studied. the change in the level of n-alkanes accumulated by the yeast during carbon starvation was determined. no differences in the rate of metabolization of 1-14c-octadecane, 1,2-3h-hexadecane, and 13-14c-pentacosane were found. during 3 hour incubation the cell losses of c25, c18 and c16 amounted to 35.0, 33.0 ... | 1980 | 7384005 |
| [role of endogenous substances in creating a background of increased radioresistance. 14. possible photoprotective mechanisms of pichia guilliermondii yeast cells against the effects of ionizing radiation]. | 1980 | 7403459 | |
| combined effect of amphotericin b and rifampin on candida species. | synergism with the combination of rifampin and amphotericin b in vitro has been demonstrated with a limited number of candida strains. to extend these studies (particularly at clinically achievable levels of rifampin), we evaluated the in vitro combined activity of serial twofold dilutions of amphotericin b (0.1 to 6.0 microgram/ml) against 11 concentrations of rifampin (0.2 to 200 microgram/ml) for 40 strains of candida: 20 candida albicans, 7 candida parapsilosis, 8 candida tropicalis, 2 candi ... | 1980 | 7425608 |
| a new neutrophil candida killing test: chromium-51 release from candida guilliermondii. | neutrophil candidacidal function was measured by a chromium-51 (51cr) release technique, using candida guilliermondii as the test organism. the effect of varying the neutrophil:candida ratio, the time course of 51cr release, the effect of different serum concentrations, and the reproducibility of the technique were investigated. results from 33 experiments, using neutrophils from 35 normal donors and six patients with known neutrophil defects, show the technique to be a reliable and reproducible ... | 1980 | 7430387 |
| [overproduction of riboflavin in mutants of pichia guilliermondii yeasts resistant to 7-methyl-8-trifluoromethyl-10-(1'-d-ribityl)isoalloxazine]. | mutants resistant to 7-methyl-8-trifluoromethyl-10-(1'-d-ribityl)isoalloxazine were selected from the yeast pichia guilliermondii, strain ms14-a10, possessing a multiple sensitivity to antibiotics and antimetabolites. a lot of such mutants displayed an elevated flavinogenic activity. the investigation of the properties of three mutants with the highest flavinogenic activity, viz. rz4, rz7 and rz11, has shown that their capability for riboflavin overproduction does not stem from disordered regula ... | 1980 | 7442566 |
| [purification and properties of the pichia guilliermondii acid nucleotide pyrophosphatase hydrolyzing flavin adeninine dinucleotide]. | acid nucleotide pyrophosphatase was isolated from the cell-free extracts of pichia guilliermondii wickerham atcc 9058. the enzyme was 25-fold purified by saturation with ammonium sulphate, gel-filtration on sephadex g-150 column and ion-exchange chromatography on deae-sephadex a-50 column. the ph optimum was 5.9, temperature optimum--45 degrees c. the enzyme catalyzed the hydrolysis of fad, nad+ and nadh, displaying the highest activity with nad+. the km, values for fad, nad+ and nadh were 1.3 x ... | 1980 | 6103593 |
| [studies on lytic enzyme activities of bacteria and streptomycetes against candida guilliermondii after partial purification with ammonium sulfate from shaking- and fermentation cultures (author's transl)]. | two bacteria and two streptomycetes strains, capable to degrade cell walls of candida guilliermondii, were isolated from soils. growth and enzyme production of these strains were investigated on synthetic and complex media in shaking culture and under fermentation conditions. the isolated microorganisms possessed lytic enzyme activities against living and autoclaved yeast cells. the lytic enzyme complex of the culture medium of microorganisms was partially purified by ammonium sulfate precipitat ... | 1979 | 44474 |
| [coordinate regulation of riboflavin permease and alpha-glucosidase synthesis in the yeast pichia guilliermondii]. | riboflavin permease of the yeast pichia guilliermondii appear to be inducible transport system. its synthesis is induced by sucrose, maltose, alpha-methyl-d-glocoside, melizitose and raffinose, but not by d-glucose, trehalose or cellobiose. the synthesis of riboflavin permease in the presence of sucrose of maltose is depressed by cycloheximide, actinomycin d and 8-hydroxyquinoline. these results suggest that the synthesis of riboflavin permease is regulated on the transcription level. the induce ... | 1979 | 116690 |
| the alkane-hydroxylating enzyme system of the yeast candida guilliermondii. | the growth of the investigated candida guilliermondii strain on n-alkanes induces an alkane-hydroxylating enzyme system, which consists of a cytochrome p-450 and a nadph-dependent reductase. the cytochrome p-450 was purified to 4 nmoles per mg protein. long-chain alkanes, preferably hexadecane to octadecane, are hydroxylated to the corresponding primary alcohol by this enzyme system. the substrate induces a type i spectrum, other compounds checked type ii spectra. | 1979 | 117657 |
| microbicidal function of the neutrophils in hairy-cell leukaemia. | the ability of neutrophils from patients with hairy-cell leukaemia to kill candida guilliermondii and staphylococcus aureus in vitro was investigated. of 10 patients, 8 showed defective candida killing, 5 defective bacteria killing, in comparison with normal controls. study of those patients who underwent splenectomy suggests that neutrophil function can be improved by splenectomy. the possible causes of defective function in this disease are discussed. | 1979 | 119411 |
| [paraffin oxidizing system of the yeast candida guilliermondii]. | it has been demonstrated that the enzyme system of candida guilliermondii responsible for hydrocarbon oxidation involves nadph-cytochrome c-reductase (ec 1623) and cytochrome p-450. the system is located in the microsomal fraction. cytochrome p-450 synthesis is induced by hexadecane occurring in the medium. the cellular content of cytochrome p-450 varies in the course of the culture growth. there is a correlation between the cellular content of cytochrome p-450 and the synthesis of primary produ ... | 1979 | 119961 |
| defective hydrogen peroxide production in chronic granulocytic leukaemia neutrophils. | hydrogen peroxide (h2o2) production by polymorphonuclear neutrophils (pmn) during phagocytosis was fluorometrically vestimated over an incubation period of 80 min during which dead candida guilliermondii were ingested. ten patients with chronic granulocytic leukaemia (cgl) were tested and compared with 15 normal subjects. the production of h2o2 by cgl neutrophils was about 29% of normal. there was no correlation between h2o2 production and the impaired killing of candida guilliermondii by the ne ... | 1979 | 283836 |
| [change in the morphological structure of the population and in the flavin level in the cells of candida guilliermondii yeasts under periodic cultivation]. | the growth of candida guilliermondii on media with glucose and acetate is described by statistical functions for cell volume (size) distribution. special instrument has been used to measure the volume of cells and volume distributions. changes in the morphological structure of populations (both in the average size of cells and in the extent of the size heterogeneity) were detected throughout the whole period of the cultural growth including the exponential phase. these changes hardly differed du ... | 1979 | 440160 |
| [biomass accumulation by polyploid forms of candida guilliermondii yeasts]. | under the action of a mitotic poison (acenaphthene), the haploid culture of candida guilliermondii assimilating n-alkanes yielded a polyploid form whose cells were four times larger, on the average, than in the haploid culture. when the pure culture was grown under stationary conditions in media with glucose, the economical coefficient, i. e. the ratio between the assimilated glucose and the accumulated biomass, was by 16, 47 and 157% higher in the polyploid culture than in the haploid one after ... | 1979 | 470627 |
| [taxonomic significance of the "hydrocarbon assimilation" trait for pichia guilliermondii wickerham yeasts]. | 1979 | 481265 | |
| [product of second-stage riboflavin biosynthesis in pichia guilliermondii]. | dialyzed extracts of the mutants of pichia guilliermondii rg80 (rib3, his-) and ra39 (rib3, ade-) in which the third step of flavinogenesis was genetically blocked converted gtp into 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate and 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine in the presence of nadph2. apparently, 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine phosphate is a true precursor of riboflavin whereas 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine is formed upon its hydro ... | 1979 | 481291 |
| [effect of polyene antibiotics and their perhydrovderivatives on intact cells and protoplasts of yeast candida guilliermondii]. | perhydroderivatives of polyene antibiotics have a much lower activity against eukaryotic cells than the polyene antibiotics itself. bacterial cells are normally resistant against most polyene antibiotics and their perhydroderivatives. in earlier experiments with wall less l-form cells of escherichia coli we have shown that the bacterial cell wall may be responsible for the resistance of the intact bacterial cells against polyene antibiotics and their perhydroderivatives by masking internal targe ... | 1979 | 483868 |
| [effect of the polyene antibiotic, roseofungin, on the liberation of the intracellular components from candida guilliermondii cells and from rat erythrocytes]. | liberation of inorganic ions (potassium and phosphorus), nucleotides and protein from the cells of candida guilliermondii and rat erythrocytes was studied. it was found that leakage of these components depended on the incubation time, antibiotic dose and amount of the cell material. the potassium ions proved to be most sensitive to the antibiotic: even at low concentrations of roseofungin (2-4 microgram/ml) the amount of the intracellular potassium changed. a decrease in the dry mass and volume ... | 1979 | 485111 |
| [genetic classification of riboflavin-dependent mutants of pichia guilliermondii yeasts]. | 114 riboflavinless mutants were selected from the genetic line of pichia guilliermondii yeast. by means of accumulation test the mutants were divided into five biochemical groups. in genetic experiments seven complementation classes were found among 106 mutants. the strains of the i biochemical group, accumulating no specific products, corresponded to complementation class rib1; ii group, accumulating 2,4,5-triaminopyrimidine - to the class rib2; iii group, accumulating 2,6-dihydroxy-4-ribitylam ... | 1979 | 488712 |
| comparison of lipid composition of candida guilliermondii grown on glucose, ethanol and methanol as the sole carbon source. | the carbon and energy source for aerobically grown cultures of candida guilliermondii profoundly influenced the neutral lipid content and the fatty acid composition of the individual lipid components. methanol (0.80%, w/v) grown cells cultivated at 30 c in presence of 0.025% ammonium sulfate contained 12% total lipids, 67% of which was neutral lipids. glucose (0.74%), w/v) or ethanol (0.53%, w/v) grown cells contained 21--22% total lipids, 80% of which was neutral lipids, under the same conditio ... | 1979 | 513982 |
| [effect of ubiquinones and their analogs on the respiratory chain enzyme activity of candida guilliermondii yeasts]. | the effect of ubiquinones with different length of their chain (coq0, coq1, coq2, coq6, coq9) and their synthetic analogues (analogues of ubiquinone-1, hexahydroubiquinone-4, monophytylquinone, diphytylquinone, triphytylquinone) on the activity of ubiquinone dependent enzyme systems was studied in mitochondrial fractions from the yeast candida guilliermondii. all of the ubiquinone homologues studied activated these systems. the synthetic analogues of ubiquinone nonspecifically inhibited the acti ... | 1979 | 530141 |
| [preliminary and postradiation effect of long-wave ultraviolet rays on cells of different species of candida irradiated with short-wave ultraviolet rays]. | certain characteristics of photoprotection induced by near-uv were studied in candida guilliermondii. candida tropicalis was also capable of photoprotection from the lethal effect of far-uv. the spectrum of action of photoprotection was recorded. the photoprotective effect was not found in candida utilis. the postradiation effect of near-uv was studied. the lethal action of far-uv was intensified in c. guilliermondii and c. tropicalis incapable of photoreactivation in contrast to c. utilis capab ... | 1979 | 572905 |
| [comparative study of the protein makeup in diploid and haploid forms of saccharomyces and pichia]. | the rates of growth, biomass accumulation, and electrophoretic spectra of mobile cytoplasmic proteins were studied with nonisogenous haploid and diploid cultures of saccharomyces cerevisiae and pichia guilliermondii as well as with isogenous haploid-diploid pairs of saccharomyces cerevisiae and pichia pinus. on a mineral medium with glucose, differences in these parameters in various yeast strains were found to be due to the genotype of a strain rather than to ploidy: nonisogenous haploid and di ... | 1979 | 384183 |
| identification of species of candida, cryptococcus, and torulopsis by gas-liquid chromatography. | gas-liquid chromatography was used to identify species of candida, cryptococcus, and torulopsis by fatty-acid analysis of the whole-cell hydrolysate. candida albicans characteristically revealed 2-oh c14:0 and c19:0 (chain length:number of double bonds); these were absent in other organisms. candida curvata was characterized by a ratio of c16:1 to c16:0 of greater than 1.0. candida guilliermondii contained c10:0, and candida tropicalis had no c12:0, these features were used for their identificat ... | 1979 | 396339 |
| affinity chromatographic purification of beta-glucosidase of candida gulliermondii. | a beta-glucosidase was isolated from candida guilliermondii, a yeast capable of growth on cellobiose. the enzyme was partially purified by treatment with polyethyleneimine and ammonium sulfate precipitation. further purification was achieved by affinity chromatography using a sepharose 4b matrix to which oxidized salicin was coupled through adipic dihydrazide. the final product was a 12.5-fold purification of the crude extract with a recovery of 27% of the initial enzyme activity. polyacrylamide ... | 1978 | 417311 |
| [structure of mannan of candida guilliermondii h]. | the mannan of candida guilliermondii was acetolyzed and the seperation by gel chromatography gave five neutral fragments with the characteristic nuclear magnetic resonance spectra, mannose, mannobiose, mannotriose, mannotetraose, and mannopentaose. all oligosaccharide fragments were composed of mannose in alpha-linkage. methylation analysis combined with gas chromatography-mass spectrometry demonstrated (1 leads to 6)-linked d-mannopyranose main chains with (1 leads to 2)-linked d-mannopyranose ... | 1978 | 645109 |
| [role of endogenous substances in creating a background of increased radioresistance. 10. the nature of the varying radioresistance of yeast cells (experiments on pichia guilliermondii of varying ploidy)]. | 1978 | 652974 | |
| [demonstration of alpha-mannosidase and beta-glucosidase in a yeast cell-wall lytic system of streptomyces spec. 5s]. | streptomyces spec. 5s accumulates in the culture fluid lytic activities against living cells of candida guilliermondii h. from the culture fluid a freeze-dried dialyzed ammonium sulfate precipitate was prepared. except beta-glucosidase-activity of alpha-mannosidase which was characterized. separation of both enzymes by chromatography on polyacrylamide gel was possible. | 1978 | 664754 |
| [electron microscopic study of the action of submicroscopic levorin structures on candida guillermondii]. | in the course of preparation of aqueous solutions of the polyene antibiotic levorin, the latter is recovered in the solid phase forming granular submicroscopic structures. if the cells of candida guilliermondii are treated with submicroscopic granular structures (smgs) of levorin, the structures are adsorbed on the surface of the yeast cell walls. some visible changes occur in the ultrastructure of the yeast cells incubated with smgs of levorin for 5 min: the inner layer of the cell wall becomes ... | 1978 | 713884 |
| defective microbicidal function of neutrophils in haematological malignancies and lymphomas: correction by levamisole in vitro. | the ability of neutrophils to kill candida guilliermondii was investigated in patients with various haematological malignancies and lymphomas. twelve of 24 patients showed significant depression of candida killing in vitro in comparison with normal controls. the addition of levamisole to the test system, in a concentration of 1 x 10(-7)m, resulted in the correction of the candida killing defect in 7 of 8 patients. | 1978 | 719129 |
| [regulation of the activity of gtp-cyclohydrolase, the enzyme of the first step of flavinogenesis in yeasts]. | the kinetic properties and regulation of activity of gtp-cyclohydrolase, the enzyme of the first step of flavinogenesis in the pichia guilliermondii yests, partially purified by gel-filtration were studied. it was found that the curve of the dependence of reaction rate on substrate concentration is non-hyperbolic. fad inhibited the enzyme activity, while riboflavin and fmn had no such effect. in addition to fad, 5'-amp, 3',5'-amp, adp, atp, nad and nadp inhibited the enzyme activity. under combi ... | 1978 | 737222 |
| [respiratory system of pichia guilliermondii yeasts with different levels of flavinogenesis]. | the yeast pichia guilliermondii was grown on media with different content of iron and its respiration system was studied. when the yeast was cultivated on a complete medium, its respiratory chain operated at the maximum rate in the exponential growth phase, i. e. all the three points of phosphorylation were involved; cytochrome oxidase was the only terminal oxidase. when the growth was decelerated and at the stationary phase, the alternative autooxidable cyanide-resistant pathway inhibited with ... | 1978 | 745565 |
| role of opportunistic fungi in ocular infections in nigeria. | the importance of opportunistic fungal pathogens in causing ocular infections is emphasized. a study was conducted over a period of 4 years (1974--1977) to investigate the role of opportunistic fungi in causing mycotic keratitis and to elucidate certain aspects of epidemiology of this disease in nigeria. fifty-nine cases of corneal ulcers of suspected mycotic etiology were investigated. fungal etiology was confirmed in 42 of these cases. the predominant etiological agent was fusarium solani in 1 ... | 1978 | 745623 |
| [effect of oxygen on pyrophosphate and atp accumulation in candida guilliermondii yeasts cultivated on petroleum paraffins]. | 1978 | 207500 | |
| [inhibition of alkaline phosphatase i of pichia guilliermondii yeast in vitro and in vivo]. | the rate of p-nitrophenyl phosphate and flavin mononucleotide (fmn) hydrolysis by the partially purified preparation of alkaline phosphatase i of pichia guilliermondii flavinogenic yeast was studied as affected by different substrates and inorganic ions. their km was established to be 2.0 x 10(-4) m and 2.5 x 10(-4) m, respectively. dephosphorylation of p-nitrophenylphosphate and fmn was inhibited competitively by beta-glycerophosphate (ki = 3.1 x 10(-3) m, respectively). the presence of inorgan ... | 1978 | 208203 |
| [substrate and inhibitory specificity of riboflavin kinase from pichia guilliermondii yeast]. | the interaction of purified riboflavin kinase (ec 2.7.1.26) from pichia guilliermondii with 44 structural vitamin b2 analogues is studied. the presence of d-ribityl lateral chain in an analogue structure is found to be necessary for the substrate activity. the substitution of ch3 groups in the 7 and 8 positions of isoalloxazine ring in the riboflavin molecule for cf3, cl, h, nh2 and n(ch3)2 resulted in the decrease of the analogue affinity to riboflavin kinase as compared with the natural substr ... | 1978 | 217452 |
| dicarboxylic acid formation by candida guilliermondii h strain h 17 during the degradation of n-tridecane in batch culture. | 1978 | 25511 | |
| cultivation of feed yeasts on media with carbohydrates. | the different culture of yeasts oxidizing hydrocarbons and the accompanying microflora are, under certain physico-chemical conditions, in metabiotic relationships which provide stability of the system. these relationships were studied with yeast cultures belonging to the candida genus and oxidizing hydrocarbons. the relationships between the hydrocarbon oxidizing yeast candida guilliermondii and other species of the genus candida were determined by a combination of active and passive antogonism, ... | 1978 | 26855 |
| cytochrome p-450 and alkane hydroxylase activity in candida guilliermondii. | in the investigated candida guilliermondii strain after growth on n-alkanes as the only carbon and energy source 5--10 nmol cytochrome p-450 per g cells (wet weight) could be detected. cytochrome p-450 and alkane hydroxylase activity was found in the 100 000 xg pellet. cofactor studies and inhibition experiments revealed the existence of a nadph-dependent cytochrome p-450 alkane hydroxylase system. | 1978 | 30246 |
| origin of the ribityl side-chain of riboflavin from the ribose moiety of guanosine triphosphate in pichia guilliermondii yeast. | in wild-type cells and some riboflavin-deficient mutants of p. guilliermondii gtp is transformed to the ribitylated intermediates 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine and 5-amino-2,6-dihydroxy-4-ribitylaminopyrimidine of the riboflavin biosynthetic path. we were able to show that these compounds were formed in vitro as well as in permeabilized cells by reactions including a reductive conversion of the product of gtp cyclohydrolase ii action upon gtp. in order to analyse the pyrimidine ... | 1978 | 30490 |
| [effect of glucose and its derivatives on systems of riboflavin uptake and excretion in the yeast pichia guilliermondii]. | riboflavin uptake by washed cells of riboflavin deficient mutant ms1-3 of pichia guilliermondii yeast was strongly depressed by d-glucose, l-sorbose, alpha-methyl-d-glucoside, sucrose, trehalose, maltose and salicin but not by d-mannose, d-galactose, d-fructose or ribitol. glucose decreased also the initial uptake rate of riboflavin analogue, 8-piperidyl-10-(1'-d-galactityl) isoalloxazine; the inhibition having a competitive character (ki==5,7 mm). apparently riboflavin permease is able to accep ... | 1978 | 32927 |
| experimental hematogenous endophthalmitis due to candida: species variation in ocular pathogenicity. | the ocular pathogenicity of species of candida other than albicans was studied in the rabbit model of hematogenous candida endophthalmitis by injection of 10(5)-10(8) colony-forming units of candida krusei, candida parapsilosis, candida guilliermondii, candida tropicalis, candida stellateoidea, and candida albicans (control). at one and three weeks after infection, rabbits were examined by indirect ophthalmoscopy and were sacrificed for culture of eyes and kidneys. with an inoculum of 10(8) orga ... | 1977 | 320277 |
| [riboflavin transport in cells of riboflavin-dependent yeast mutants]. | riboflavin was transported at a high rate into yeast cells of pichia guilliermondii and schwanniomyces occidentalis mutants capable of growth in a medium containing low concentrations of riboflavin, and having multiple susceptibility to some antibiotics and antimetabolites. sucrose and sodium azide inhibited transport of riboflavin. other riboflavin dependent mutants of pichia guilliermondii, pichia ohmeri, torulopsis candida, and saccharomyces cerevisiae, also growing in media containing low co ... | 1977 | 329070 |
| [activities of some yeast flavogenic enzymes in situ]. | effects of digitonin, dimethylsulfoxide and protamine sulfate on yeast pichia guilliermondii were studied in order to produce cells with increased permeability and possessing the gtp-cyclohydrolase, riboflavinsynthetase and riboflavinkinase activities. the digitonin-treated cells exhibited a higher cyclohydrolase activity than the cell-free extracts; the activities of riboflavinsynthetase and riboflavinkinase in the cells and cell-free extracts were found to be similar. treatment of cells with d ... | 1977 | 199288 |
| impaired neutrophil function and myeloperoxidase deficiency in myeloid metaplasia. | the ability of neutrophils to phagocytose and kill candida guilliermondii was investigated in 12 patients with myeloid metaplasia (mm). following ingestion there was a considerable impairment in the ability of mm neutrophils to kill and digest candida which was not explained by the very mild impairment in phagocytosis. quantitative myeloperoxidase measurement revealed an overall deficiency of this enzyme in mm neutrophils and a highly significant correlation between low myeloperoxidase levels an ... | 1977 | 203312 |
| [regulation of synthesis of gtp-cyclohydrolase participating in yeast falvinogenesis by iron]. | pichia guilliermondii, schwanniomyces occidentalis, torulopsis candida and several riboflavin-dependent mutants of torulopsis candida were grown in a medium with a low concentration of iron. in these conditions, the activity of gtp-cyclohydrolase which catalyzes the first step of flavinogenesis increases. the activity of the enzyme increases also when the cells of t. candida and p. guilliermondii with a high content of iron are incubated with alpha, alpha'-dipyridyl which induces overproduction ... | 1977 | 561294 |
| repair of 313-nm induced lesions and photoprotection in yeast candida guilliermondii. | 1977 | 594172 |