Publications
Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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inhibitory effects of bromelain, a cysteine protease derived from pineapple stem (ananas comosus), on intestinal motility in mice. | bromelain (br) is a cysteine protease with inhibitory effects on intestinal secretion and inflammation. however, its effects on intestinal motility are largely unexplored. thus, we investigated the effect of this plant-derived compound on intestinal contractility and transit in mice. | 2011 | 21689210 |
genomic analysis of grapevine leafroll associated virus-5 and related viruses. | the grapevine leafroll-associated viruses (glravs) (closteroviridae) represent an emerging threat to world grape production. one group of glravs within the genus ampelovirus, the glrav-4-like viruses (glrav-4lvs), contains a fragmented collection of seven viruses only two of which (glrav-pr and glracv) are fully sequenced. here in reporting the sequence of glrav-5, a member of glrav-4lvs, we identify genomic elements common to the glrav-4lv group. exclusive properties include a highly conserved ... | 2011 | 21893115 |
a comparative study of ph modulation and trace elements of various fruit juices on enamel erosion: an in vitro study. | fruit juices are popular worldwide with children of all ages as they are sweet and perceived to be healthful. this in vitro study was sought to measure ph of 10 different fruit juices, to find out possible erosive effects on human dental enamel of 40 extracted sound premolars and also to measure fluoride and trace elements of these juices. the estimation of ph of fruit juices was done by using systronic uph 362 ph meter. the erosive effects of fruit juices were tested by using polarized light mi ... | 2011 | 21985876 |
use of a multivariate approach to assess the incidence of alicyclobacillus spp. in concentrate fruit juices marketed in argentina: results of a 14-year survey. | the purpose of this study was to determine the incidence of alicyclobacillus spp. in fruit/vegetable juices (concentrated pulps and clarified and non-clarified juices) marketed in argentina between 1996 and 2009. the presence of alicyclobacillus was determined in a total of 8556 samples of fruit and vegetable juices (apple, pear, grape, peach, blend of juices, tangerine, pineapple, orange, mango, plum, guava, apricot, lemon, banana, kiwi, carrot, strawberry, grapefruit, and beetroot) collected i ... | 2011 | 21962940 |
juice blends--a way of utilization of under-utilized fruits, vegetables, and spices: a review. | the post-harvest shelf life of maximum of fruits and vegetables is very limited due to their perishable nature. in india more then 20-25 percent of fruits and vegetables are spoiled before utilization. despite being the world's second largest producer of fruits and vegetables, in india only 1.5 percent of the total fruits and vegetables produced are processed. maximum amounts of fruit and vegetable juices turn bitter after extraction due to conversion of chemical compounds. in spite of being und ... | 2011 | 21929332 |
studies on production of alcoholic beverages from some tropical fruits. | the varieties of alcoholic beverages from watermelon; watermelon-banana and watermelon-pineapple mixtures were produced by using monoculture and mixed culture fermentation techniques. three yeast species, namely, saccharomyces cerevisiae, kleochera apicalata, torulospora delbruckii and four bacterial species leuconostoc oenos, lactobacillus sp, micrococcus luteus and streptococcus lactis were identified during the study. the daily succession of these organisms in the various fermenting samples d ... | 2010 | 22815578 |
the role of aqueous extract of pineapple fruit parts on the healing of acute crush tendon injury. | the pineapple plant contains the enzyme bromelain which has been acclaimed to reduce pain and swellings following acute muscle injuries as well as carotenoids and polyphenols which are powerful antioxidants. it is yet to be determined if these constituents are distributed throughout the plant and what effect they have on the healing of acute tendon injuries. | 2010 | 21913532 |
fruits and vegetables protect against the genotoxicity of heterocyclic aromatic amines activated by human xenobiotic-metabolizing enzymes expressed in immortal mammalian cells. | heterocyclic aromatic amines (haas) can be formed during the cooking of meat and fish at elevated temperatures and are associated with an increased risk for cancer. on the other hand, epidemiological findings suggest that foods rich in fruits and vegetables can protect against cancer. in the present study three teas, two wines, and the juices of 15 fruits and 11 vegetables were investigated for their protective effect against the genotoxic effects of 2-amino-3-methylimidazo[4,5-f]quinoline (iq) ... | 2010 | 20713174 |
evaluation of anti-quorum-sensing activity of edible plants and fruits through inhibition of the n-acyl-homoserine lactone system in chromobacterium violaceum and pseudomonas aeruginosa. | to find out an alternative strategy to antibiotic usage against bacterial infection. | 2010 | 20720417 |
sensory profile of a model energy drink with varying levels of functional ingredients-caffeine, ginseng, and taurine. | energy drinks have increased in popularity in recent years due to the claimed energy boost provided by functional ingredients. a multitude of functional ingredients have been utilized; however, there is limited research on their sensory effects in energy drink formulations. a 13-member descriptive analysis panel was conducted to investigate the effects on the sensory and rheological properties of 3 common functional ingredients-caffeine, ginseng, and taurine-in a noncarbonated model energy drink ... | 2010 | 20722948 |
alicyclobacillus acidoterrestris in pasteurized exotic brazilian fruit juices: isolation, genotypic characterization and heat resistance. | in this study, the population of alicyclobacillus spp. was estimated in pasteurized exotic brazilian fruit juices using the most probable number (mpn) technique followed by biochemical tests. pasteurized passion fruit (n = 57) and pineapple (n = 50) juices were taken directly from brazilian manufacturers. while alicyclobacillus spp. was isolated from passion fruit juice, the microorganism was not found in any pineapple juice samples. a higher incidence of alicyclobacillus was observed in samples ... | 2010 | 20832679 |
dietary supplementation with fresh pineapple juice decreases inflammation and colonic neoplasia in il-10-deficient mice with colitis. | bromelain, a mixture of proteolytic enzymes typically derived from pineapple stem, decreases production of proinflammatory cytokines and leukocyte homing to sites of inflammation. we previously showed that short-term oral treatment with bromelain purified from pineapple stem decreased the severity of colonic inflammation in c57bl/6 il10(-/-) mice with chronic colitis. since fresh pineapple fruit contains similar bromelain enzymes but at different proportions, this study aimed to determine whethe ... | 2010 | 20848493 |
fusarium ananatum sp. nov. in the gibberella fujikuroi species complex from pineapples with fruit rot in south africa. | pineapple (ananas comosus) is native to south america and widely planted as a fruit crop in the tropics and sub-tropics. this plant is susceptible to a number of fungal diseases of which the most severe is fusariosis. the disease is caused by fusarium guttiforme and occurs only in south and central america. the occurrence of a similar disease on pineapples in south africa has prompted a re-evaluation of the fusarium sp. associated with pineapple fruit rot. phylogenetic relationships of isolates ... | 2010 | 20943163 |
specificity of monoclonal antibodies to strains of dickeya sp. that cause bacterial heart rot of pineapple. | during a severe outbreak of bacterial heart rot that occurred in pineapple plantations on oahu, hawaii, in 2003 and years following, 43 bacterial strains were isolated from diseased plants or irrigation water and identified as erwinia chrysanthemi (now dickeya sp.) by phenotypic, molecular, and pathogenicity assays. rep-pcr fingerprint patterns grouped strains from pineapple plants and irrigation water into five genotypes (a-e) that differed from representatives of other dickeya species, pectoba ... | 2010 | 21050038 |
development of a yeast strain for xylitol production without hydrolysate detoxification as part of the integration of co-product generation within the lignocellulosic ethanol process. | the present study verified an applicable technology of xylitol bioconversion as part of the integration of co-product generation within second-generation bioethanol processes. a newly isolated yeast strain, candida tropicalis jh030, was shown to have a capacity for xylitol production from hemicellulosic hydrolysate without detoxification. the yeast gives a promising xylitol yield of 0.71 g(p) g(s)(-1) from non-detoxified rice straw hydrolysate that had been prepared by the dilute acid pretreatme ... | 2010 | 21095119 |
cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars. | fusariosis, caused by the fungus fusarium subglutinans f. sp. ananas (syn. f. guttiforme), is one of the main phytosanitary threats to pineapple (ananas comosus var. comosus). identification of plant cell responses to pathogens is important in understanding the plant-pathogen relationship and establishing strategies to improve and select resistant cultivars. studies of the structural properties and phenolic content of cell walls in resistant (vitoria) and susceptible (perola) pineapple cultivars ... | 2010 | 20607243 |
optimised separation procedures for the simultaneous assay of three plant hormones in liquid biofertilisers. | the overuse of petrochemical-based synthetic fertilisers has caused detrimental effects to soil, water supplies, foods and animal health. this, in addition to increased awareness of organic farming, has generated considerable interest in the evaluation of renewable biofertilisers. | 2010 | 19845039 |
qualitative data analysis for an exploratory sensory study of grechetto wine. | grechetto is a traditional white-grape vine, widespread in umbria and lazio regions in central italy. despite the wine commercial diffusion, little literature on its sensory characteristics is available. the present study is an exploratory research conducted with the aim of identifying the sensory markers of grechetto wine and of evaluating the effect of clone, geographical area, vintage and producer on sensory attributes. a qualitative sensory study was conducted on 16 wines, differing for vint ... | 2010 | 20103144 |
molecular phylogenetic diversity of dermatologic and other human pathogenic fusarial isolates from hospitals in northern and central italy. | fifty-eight fusaria isolated from 50 italian patients between 2004 and 2007 were subject to multilocus dna sequence typing to characterize the spectrum of species and circulating sequence types (sts) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in northern and central italy. sequence typing revealed that the isolates were nearly evenly divided among the fusarium solani species complex (fssc; n = 18), the f. oxysporum species complex (fo ... | 2010 | 20107100 |
prevalence, concentration, spoilage, and mitigation of alicyclobacillus spp. in tropical and subtropical fruit juice concentrates. | the presence of alicyclobacillus in fruit juices and concentrates poses a serious problem for the juice industry. this study was undertaken to determine the (i) prevalence, concentration, and species of alicyclobacillus in tropical and subtropical concentrates; (ii) efficacy of aqueous chlorine dioxide in reducing alicyclobacillus spp. spores on tropical and subtropical fruit surfaces; and (iii) fate of and off-flavor production by alicyclobacillus acidoterrestris in mango and pineapple juices. ... | 2010 | 21356453 |
3'-coterminal subgenomic rnas and putative cis-acting elements of grapevine leafroll-associated virus 3 reveals 'unique' features of gene expression strategy in the genus ampelovirus. | the family closteroviridae comprises genera with monopartite genomes, closterovirus and ampelovirus, and with bipartite and tripartite genomes, crinivirus. by contrast to closteroviruses in the genera closterovirus and crinivirus, much less is known about the molecular biology of viruses in the genus ampelovirus, although they cause serious diseases in agriculturally important perennial crops like grapevines, pineapple, cherries and plums. | 2010 | 20682046 |
transfer of pantoea citrea, pantoea punctata and pantoea terrea to the genus tatumella emend. as tatumella citrea comb. nov., tatumella punctata comb. nov. and tatumella terrea comb. nov. and description of tatumella morbirosei sp. nov. | pantoea citrea, pantoea punctata and pantoea terrea were described for strains isolated from fruit and soil originating in japan. these three 'japanese' species have been shown to be phylogenetically distant from other species of the genus pantoea. it has been observed previously that, using multilocus sequence analysis (mlsa), the 'japanese' species consistently formed a distinct clade with an extended branch length, casting doubt on the inclusion of these species within the genus pantoea. furt ... | 2010 | 19654354 |
bromelain's activity and potential as an anti-cancer agent: current evidence and perspectives. | the medicinal qualities of pineapple are recognized in many traditions in south america, china and southeast asia. these qualities are attributed to bromelain, a 95%-mixture of proteases. medicinal qualities of bromelain include anti-inflammatory, anti-thrombotic, fibrinolytic and anti-cancer functions. existing evidence derived from clinical observations as well as from mouse- and cell-based models suggests that bromelain acts systemically, affecting multiple cellular and molecular targets. in ... | 2010 | 19700238 |
volatile antimicrobials from muscodor crispans, a novel endophytic fungus. | muscodor crispans is a recently described novel endophytic fungus of ananas ananassoides (wild pineapple) growing in the bolivian amazon basin. the fungus produces a mixture of volatile organic compounds (vocs); some of the major components of this mixture, as determined by gc/ms, are propanoic acid, 2-methyl-, methyl ester; propanoic acid, 2-methyl-; 1-butanol, 3-methyl-;1-butanol, 3-methyl-, acetate; propanoic acid, 2-methyl-, 2-methylbutyl ester; and ethanol. the fungus does not, however, pro ... | 2010 | 19797357 |
pilot-scale removal of chromium from industrial wastewater using the chromebac system. | the enzymatic reduction of cr(vi) to cr(iii) by cr(vi) resistant bacteria followed by chemical precipitation constitutes the chromebac system. acinetobacter haemolyticus was immobilized onto carrier material inside a 0.2m(3) bioreactor. neutralized electroplating wastewater with cr(vi) concentration of 17-81 mg l(-1) was fed into the bioreactor (0.11-0.33 m(3)h(-1)). complete cr(vi) reduction to cr(iii) was obtained immediately after the start of bioreactor operation. together with the flocculat ... | 2010 | 20185301 |
fate of escherichia coli o157:h7 and salmonella on fresh and frozen cut pineapples. | pineapples have been associated with outbreaks of escherichia coli and salmonella. little is known about the behavior of e. coli o157:h7 and salmonella on cut pineapple. the objectives of this study were to evaluate the fate of e. coli o157:h7 and salmonella on fresh and frozen cut pineapples and the influence of acid adaptation on salmonella survival on fresh cut pineapple at ambient storage (23 degrees c). cut pineapples were spot inoculated with a four-strain cocktail of e. coli o157:h7 or a ... | 2010 | 20202325 |
taxonomic structure of the yeasts and lactic acid bacteria microbiota of pineapple (ananas comosus l. merr.) and use of autochthonous starters for minimally processing. | pichia guilliermondii was the only identified yeast in pineapple fruits. lactobacillus plantarum and lactobacillus rossiae were the main identified species of lactic acid bacteria. typing of lactic acid bacteria differentiated isolates depending on the layers. l. plantarum 1or12 and l. rossiae 2mr10 were selected within the lactic acid bacteria isolates based on the kinetics of growth and acidification. five technological options, including minimal processing, were considered for pineapple: heat ... | 2010 | 20227603 |
transcript analysis of parasitic females of the sedentary semi-endoparasitic nematode rotylenchulus reniformis. | rotylenchulus reniformis, the reniform nematode, is a sedentary semi-endoparasitic nematode capable of infecting >300 plant species, including a large number of crops such as cotton, soybean, and pineapple. in contrast to other economically important plant-parasitic nematodes, molecular genetic data regarding the r. reniformis transcriptome is virtually nonexistant. herein, we present a survey of r. reniformis ests that were sequenced from a sedentary parasitic female cdna library. cluster analy ... | 2010 | 20346373 |
genome sequence of pantoea ananatis lmg20103, the causative agent of eucalyptus blight and dieback. | pantoea ananatis is a gram-negative plant pathogen that causes disease on a broad range of host plants, including pineapple, maize, rice, onion, melons, and eucalyptus, and has been implicated in several cases of human disease. here, we report the genome sequence of p. ananatis lmg20103 isolated from diseased eucalyptus in south africa. | 2010 | 20348253 |
utilization of wastewater originated from naturally fermented virgin coconut oil manufacturing process for bioextract production: physico-chemical and microbial evolution. | production of virgin coconut oil via natural fermentation has led to large amount of wastes being generated, i.e., coconut pulp and wastewater containing coconut crème. objective of this study is to gain more insight into the feasibility of utilization of such wastes as raw materials together with several types of wastes such as fish waste and/or pineapple peel for bioextract production. chemical, physico-chemical and biological changes including phytotoxicity of the fermented mixture were close ... | 2010 | 20362440 |
effects of acetic acid, ethanol, and so(2) on the removal of volatile acidity from acidic wines by two saccharomyces cerevisiae commercial strains. | herein, we report the influence of different combinations of initial concentration of acetic acid and ethanol on the removal of acetic acid from acidic wines by two commercial saccharomyces cerevisiae strains s26 and s29. both strains reduced the volatile acidity of an acidic wine (1.0 gl(-1) acetic acid and 11% (v/v) ethanol) by 78% and 48%, respectively. acetic acid removal by strains s26 and s29 was associated with a decrease in ethanol concentration of 0.7 and 1.2% (v/v), respectively. strai ... | 2010 | 20390413 |
autochthonous yeasts associated with mature pineapple fruits, freshly crushed juice and their ferments; and the chemical changes during natural fermentation. | this study investigated autochthonous yeasts and their functions in the spontaneous fermentation of freshly crushed pineapple juice samples collected from two different areas of both thailand and australia. hanseniaspora uvarum and pichia guilliermondii were the main yeast species observed on the fruit skins of thai samples, and also in the fresh juice and ferments of all samples from both countries. p. guilliermondii was consistently present as the dominant species during the early stage of the ... | 2010 | 20462752 |
modification of pineapple peel fiber as metal ion adsorbent through reaction with succinic anhydride in pyridine and dimethyl sulfoxide solvents. | reactions between saponified pineapple peel fiber (sppf) and succinic anhydride were performed in refluxed pyridine and dimethyl sulfoxide to obtain modified pineapple peel fiber in pyridine (mppf-py) and modified pineapple peel fiber in dimethyl sulfoxide at room temperature (mppf-dmrt) and at 70 degrees c (mppf-dm70) as novel metal ionic adsorbents. the modified pineapple peel fibers were characterized by fourier transform infrared (ftir) and x-ray diffraction (xrd). the mppf-py, mppf-dmrt, an ... | 2010 | 20853752 |
online intelligent controllers for an enzyme recovery plant: design methodology and performance. | this paper focuses on the development of intelligent controllers for use in a process of enzyme recovery from pineapple rind. the proteolytic enzyme bromelain (ec 3.4.22.4) is precipitated with alcohol at low temperature in a fed-batch jacketed tank. temperature control is crucial to avoid irreversible protein denaturation. fuzzy or neural controllers offer a way of implementing solutions that cover dynamic and nonlinear processes. the design methodology and a comparative study on the performanc ... | 2010 | 21234106 |
[determination of amitrole in agricultural products by high performance liquid chromatography-tandem mass spectrometry]. | a high performance liquid chromatography-tandem mass spectrometry method (hplc-ms/ms) was developed for the analysis of amitrole residues in agricultural products. the samples were extracted by 25% acetone for wheat, fish, pork and liver samples, 1% acetic acid-25% acetone for maize and peanut samples, 1% acetic acid solution for honeysuckle, the powder of ginger, the powder of bunge prickly ash and tea leaves samples, 1% acetic acid solution-dichloromethane for apple, pineapple, spinach, carrot ... | 2010 | 20549983 |
sulfur-containing constituents and one 1h-pyrrole-2-carboxylic acid derivative from pineapple [ananas comosus (l.) merr.] fruit. | two sulfur-containing compounds, (s)-2-amino-5-((r)-1-carboxy-2-((e)-3-(4-hydroxy-3-methoxyphenyl)allylthio)ethyl-amino)-5-oxopentanoic acid (1) and (s)-2-amino-5-((r)-1-(carboxymethylamino)-3-((e)-3-(4-hydroxyphenyl)allylthio)-1-oxopropan-2-ylamino)-5-oxopentanoic acid (2), and one 1h-pyrrole-2-carboxylic acid derivative, 6-(3-(1h-pyrrole-2-carbonyloxy)-2-hydroxypropoxy)-3,4,5-trihydroxy-tetrahydro-2h-pyran-2-carboxylic acid (3), together with eighteen known phenolic compounds, were isolated fr ... | 2010 | 20843530 |
degradability of fluorapatite-leucite ceramics in naturally acidic agents. | this study was conducted to evaluate the titratable acidity and effect of naturally acidic agents on the surface microhardness, elemental composition, and surface morphology of fluorapatite-leucite ceramics. one hundred and ten ceramic disks (ips d.sign), 12.0 mm in diameter and 2.0 mm in thickness, were fabricated. before immersion, the baseline data of vickers microhardness and elemental composition were recorded. four groups were immersed in acidic agents (citrate buffer solution, green mango ... | 2010 | 20827032 |
chemical durability and microhardness of dental ceramics immersed in acidic agents. | to evaluate the microhardness and surface elemental compositions of ceramics immersed in acidic agents. | 2010 | 19878041 |
glycemic index and glycemic load of selected chinese traditional foods. | to determine the glycemic index (gi) and glycemic load (gl) values of chinese traditional foods in hong kong. | 2010 | 20333793 |
influence of modified atmosphere packaging on volatile compounds and physicochemical and antioxidant attributes of fresh-cut pineapple (ananas comosus). | the effects of modified atmosphere packaging on volatile compound content and physicochemical and antioxidant attributes of gold cultivar fresh-cut pineapples were assessed throughout storage at 5 degrees c. fresh-cut pineapple pieces were packed under lo (low oxygen, 12% o(2), 1% co(2)), air (20.9% o(2)) and ho (high oxygen, 38% o(2)) headspace atmospheres. methyl butanoate, methyl 2-methylbutanoate, and methyl hexanoate were the most abundant volatiles regardless of the packaging atmosphere an ... | 2010 | 20225823 |
the effect of acidic agents on surface ion leaching and surface characteristics of dental porcelains. | acidic food and sour fruits and drinks have been investigated for their destructive effects on enamel. however, their effect on porcelain restorations has not been widely examined. | 2010 | 20188237 |
composition of essential oil of aerial parts of chamomilla suaveolens from estonia. | variations in the essential oil composition of aerial parts of pineapple weed (chamomilla suaveolens (pursh) rydb.) growing wild in estonia, were determined using gc/fid and gc/ms. forty-four components were identified, representing over 90% of the total oil. nine compounds have not been mentioned in the literature before. the principal biologically active compounds in c. suaveolens oils were (z)-en-yne-dicycloether (17.0-40.7%), (e)-beta-farnesene (19.5-32.2%), geranyl isovaleriate (8.4-18.4%), ... | 2010 | 20184038 |
nitric oxide mediates the hormonal control of crassulacean acid metabolism expression in young pineapple plants. | genotypic, developmental, and environmental factors converge to determine the degree of crassulacean acid metabolism (cam) expression. to characterize the signaling events controlling cam expression in young pineapple (ananas comosus) plants, this photosynthetic pathway was modulated through manipulations in water availability. rapid, intense, and completely reversible up-regulation in cam expression was triggered by water deficit, as indicated by the rise in nocturnal malate accumulation and in ... | 2010 | 20147491 |
formation of shaped barium sulfate-dye hybrids: waste dye utilization for eco-friendly treatment of wastewater. | owing to the present complexity and difficulty of concentrated dye wastewater treatment, this work aimed to synthesize a reproducible waste-sorbing material for the treatment of wastewater by forming the dye-conjugating complex hybrid. | 2010 | 19844752 |
surface characteristic changes of dental ceramics after cyclic immersion in acidic agents and titratable acidity. | the potential erosive effect of acidic food, sour fruits and drinks on all-ceramic restorations used in dentistry has not been clearly documented. surface characteristic changes have been evaluated and compared for disc-shaped specimens (diameter 12.0 mm and thickness 2.0 mm) of fluorapatite-leucite and fluorapatite ceramics using various storage agents (deionized water, citrate buffer solution, pineapple juice, green mango juice, cola soft drink and 4% acetic acid). immersion in pineapple juice ... | 2010 | 21265437 |
simultaneous analysis of amino acids and carboxylic acids by capillary electrophoresis-mass spectrometry using an acidic electrolyte and uncoated fused-silica capillary. | a simple, low-cost capillary electrophoresis-mass spectrometry (ce-ms) method is demonstrated for the simultaneous analysis of amino acids and small carboxylic acids (glycerate, lactate, fumarate, succinate, malate, tartrate, citrate, iso-citrate, cis-aconitate, and shikimate). all ce-ms experiments were performed using a single uncoated fused-silica capillary and with a single separation electrolyte, formic acid. for ce polarity, the ce inlet was set as the anode, and the ms side was set as the ... | 2010 | 21080676 |
improved characterization of the botanical origin of sugar by carbon-13 snif-nmr applied to ethanol. | until now, no analytical method, not even isotopic ones, had been able to differentiate between sugars coming from c4-metabolism plants (cane, maize, etc.) and some crassulacean acid metabolism plants (e.g., pineapple, agave) because in both cases the isotope distributions of the overall carbon-13/carbon-12 and site-specific deuterium/hydrogen isotope ratios are very similar. following recent advances in the field of quantitative isotopic carbon-13 nmr measurements, a procedure for the analysis ... | 2010 | 21028824 |
thermoperiod affects the diurnal cycle of nitrate reductase expression and activity in pineapple plants by modulating the endogenous levels of cytokinins. | nitrate reductase (nr, ec 1.6.6.1) activity in higher plants is regulated by a variety of environmental factors and oscillates with a characteristic diurnal rhythm. in this study, we have demonstrated that the diurnal cycle of nr expression and activity in pineapple (ananas comosus, cv. smooth cayenne) can be strongly modified by changes in the day/night temperature regime. plants grown under constant temperature (28 degrees c light/dark) showed a marked increase in the shoot nr activity (nra) d ... | 2009 | 19832938 |
vicker's microhardness and energy dispersive x-ray analysis of fluorapatite-leucite and fluorapatite ceramics cyclically immersed in acidic agents. | this study evaluated the microhardness and elemental composition of various ceramics immersed in acidic agents. one hundred and five discs of fluorapatite-leucite (ips d.sign) and fluorapatite ceramics (ips e.max ceram) were randomly divided into 7 groups of 15 each. five groups were alternately immersed in acidic agents (citrate buffer solution, pineapple juice, green mango juice, cola soft drink and 4% acetic acid) for 30 min and deionized water for 5 min in 7 cycles at 37 degrees c. two group ... | 2009 | 19776513 |
structure, stability, and thermochemistry of the fullerene derivatives c(64)x(6) (x = h, f, cl). | the geometrical structures, electronic properties, and stabilities of the unconventional fullerene derivatives c(64)x(6) (x = h, f, cl) have been systematically studied by the first-principle calculations based on the density functional theory. the fullerene derivatives 1911(2)-c(64)x(6) generated from the pineapple-shaped c(64)x(4) are predicted to possess the lowest energies. the other two x atoms are added to the carbon atoms with the highest local strain assessed by the pyramidalization angl ... | 2009 | 19757780 |
an extended ae-rich n-terminal trunk in secreted pineapple cystatin enhances inhibition of fruit bromelain and is posttranslationally removed during ripening. | phytocystatins are potent inhibitors of cysteine proteases and have been shown to participate in senescence, seed and organ biogenesis, and plant defense. however, phytocystatins are generally poor inhibitors of the cysteine protease, bromelain, of pineapple (ananas comosus). here, we demonstrated that pineapple cystatin, accys1, inhibited (>95%) stem and fruit bromelain. accys1 is a unique cystatin in that it contains an extended n-terminal trunk (ntt) of 63 residues rich in alanine and glutama ... | 2009 | 19648229 |
the prevalence of food hypersensitivity in young adults. | rising prevalence of food hypersensitivity (fhs) and severe allergic reactions to foods have been reported in the last decade. however, little is known on the prevalence in young adults. this study estimated the prevalence of fhs to the most common allergenic foods in an unselected population of young adults. we investigated a cohort of 1272 young adults 22 years of age by questionnaire, skin prick test (spt) and histamin release (hr) followed by oral challenge to the most common allergenic food ... | 2009 | 19594854 |
detection of urease in the cell wall and membranes from leaf tissues of bromeliad species. | urea is an important nitrogen source for some bromeliad species, and in nature it is derived from the excretion of amphibians, which visit or live inside the tank water. its assimilation is dependent on the hydrolysis by urease (ec: 3.5.1.5), and although this enzyme has been extensively studied to date, little information is available about its cellular location. in higher plants, this enzyme is considered to be present in the cytoplasm. however, there is evidence that urease is secreted by the ... | 2009 | 19508368 |
the pineapple upside-down liver cake. | 2009 | 19479784 | |
screening of antiproliferative effect of aqueous extracts of plant foods consumed in méxico on the breast cancer cell line mcf-7. | we evaluated the antiproliferative effect of aqueous extracts of 14 plant foods consumed in mexico on the breast cancer cell line mcf-7. the plant foods used were avocado, black sapote, guava, mango, prickly pear cactus stems (called nopal in mexico, cooked and raw), papaya, pineapple, four different cultivars of prickly pear fruit, grapes and tomato. β-carotene, total phenolics and gallic acid contents and the antioxidant capacity, measured by the ferric reducing/antioxidant power and the 2,2-d ... | 2009 | 19468947 |
removal of methylene blue from aqueous solution by adsorption onto pineapple leaf powder. | the ability of an unconventional bio-adsorbent, pineapple leaf powder (plp) for the adsorption of methylene blue (mb) from aqueous solution was studied. it was observed that intra-particle diffusion was involved in the adsorption process and that the kinetic data fitted well with a pseudo-second-order equation. fitting parameters revealed that the rate of adsorption increased with decrease in dye concentration and decrease in ionic strength while the mixing speed did not have a significant effec ... | 2009 | 19447547 |
[the characteristic analysis of several mineral contents in chinese orange juice]. | a study was carried out on the contents of mineral elements such as k, ca, na, and mg in seven different orange varieties, namely pineapple orange, hamlin, trovita, jincheng, 1,232 tangor, olinda valencia and delta valencia, by flame atomic absorption spectrometry. the results indicated that the content sequence of different nutritional elements was k > mg > ca > na, with a range of 1,233.75-1,866.23, 77.51-167.15, 49.32-125.29 and 1.22-9.26 mg x l(-1) respectively. the range scale of the four e ... | 2009 | 19385253 |
pineapple and banana pectins comprise fewer homogalacturonan building blocks with a smaller degree of polymerization as compared with yellow passion fruit and lemon pectins: implication for gelling properties. | pectins are viewed as multiblock cobiopolymers of different pectic polysaccharides, notably, homogalacturonan (hg) and rhamnogalacturonan i (rg i). furthermore, on the basis of hgs isolated from different (pectins from) dicot cell walls, hg is supposed to have an average degree of polymerization (dp) of approximately 100 irrespective of the plant source. to validate or invalidate these suppositions, pectins from both monocot (pineapple and banana) and dicot (yellow passion fruit and lemon) cell ... | 2009 | 19260678 |
soft fruit traceability in food matrices using real-time pcr. | food product authentication provides a means of monitoring and identifying products for consumer protection and regulatory compliance. there is a scarcity of analytical methods for confirming the identity of fruit pulp in products containing soft fruit. in the present work we have developed a very sensible qualitative and quantitative method to determine the presence of berry dnas in different food matrices. to our knowledge, this is the first study that shows the applicability, to soft fruit tr ... | 2009 | 22253987 |
the effects of concentrate added to pineapple (ananas comosus linn. mer.) waste silage in differing ratios to form complete diets, on digestion, excretion of urinary purine derivatives and blood metabolites in growing, male, thai swamp buffaloes. | four, male, growing thai swamp buffaloes (197 +/- 5.3 kg and all 1 year old) were used to evaluate the effects of concentrate added to pineapple waste silage in differing ratios, to form a complete diet, studying in vivo digestion, the rate of passage, microbial protein synthesis and blood metabolites. animals were fed ad libitum with 4 diets, using four combinations of pineapple waste silage (p) and concentrate (c), in the proportions (on a dry matter basis) of 0.8:0.2 (p80:c20), 0.6:0.4 (p60:c ... | 2009 | 18649000 |
screening of trichoderma isolates as a biological control agent against ceratocystis paradoxa causing pineapple disease of sugarcane. | in this study, dual culture, poison agar, and direct methods were used to assess the ability of trichoderma virens imi-392430, t. pseudokoningii imi-392431, t. harzianum imi-392432, t. harzianum imi-392433, and t. harzianum imi-392434 to control ceratocystis paradoxa, which causes the pineapple disease of sugarcane. the highest percentage inhibition of radial growth (pirg) values were observed with t. harzianum imi-392432 using two dual culture methods, 63.80% in method i and 80.82% in method ii ... | 2009 | 23983548 |
matrix effects observed during pesticides residue analysis in fruits by gc. | the influence of the sample matrix in the gc-electron-capture detection analysis of the pesticides dimethoate, diazinon, chlorothalonil, parathion methyl and fenitrothion in fruits samples has been studied. experiments have been carried out where the pesticide responses in standard solutions prepared in selected solvent were compared with their response when present in apple, mango, papaya, banana, pineapple and melon extracts. the presence of matrix effects (mes) and their extent were shown to ... | 2009 | 19877144 |
oriented cell division as a response to cell death and cell competition. | the imaginal disc epithelia that give rise to the adult ectoderm of drosophila can compensate to produce normal adult organs after damage. we looked at the local response to cell death by using two genetic methods to elevate cell death rates. during cell competition, sporadic cell death occurs predictably along the boundaries between populations of competing wild-type and "minute" cells (m/+). boundaries between m/+ and wild-type populations show an unusual degree of mixing, associated with mito ... | 2009 | 19853449 |
complementary therapies: your questions answered. use of pineapple for induction of labour. | 2009 | 19899285 | |
factors affecting migration of vanillin from chitosan/methyl cellulose films. | the diffusion kinetics and factors affecting the migration of vanillin from chitosan/methyl cellulose (chi/mc) films into water, cantaloupe juice (cj), pineapple juice (pj), and citrate buffer adjusted to ph values of 3.5, 5, and 6.5 were studied. vanillin was incorporated into the chi/mc films to provide an inhibitory effect against microorganisms. initial vanillin concentration in the films, temperature, and ph of extracting solvent impacted the migration behavior of vanillin. the diffusion co ... | 2009 | 19895459 |
chemical examination of citrus sinensis flavedo variety pineapple. | phytochemical examination of citrus sinensis flavedo var. pineapple resulted in the isolation of six compounds characterized as tetracosane, ethyl pentacosanoate, tetratriacontanoic acid, tangertin, beta-sitosteryl-beta-d-glucoside and 3,5,4'-trihydroxy-7,3'-dimethoxy flavanone 3-o-beta-glucoside. of these 3,5,4'-trihydroxy-7,3'-dimethoxy flavanone 3-o-beta-glucoside is a hitherto unreported compound. | 2009 | 20376223 |
a novel agricultural waste adsorbent for the removal of cationic dye from aqueous solutions. | in this paper, pineapple stem (ps) waste, an agricultural waste available in large quantity in malaysia, was utilized as low-cost adsorbent to remove basic dye (methylene blue, mb) from aqueous solution by adsorption. batch mode experiments were conducted at 30 degrees c to study the effects of initial concentration of methylene blue, contact time and ph on dye adsorption. equilibrium adsorption isotherms and kinetic were investigated. the experimental data were analyzed by the langmuir and freu ... | 2009 | 18573607 |
trace metal levels in fruit juices and carbonated beverages in nigeria. | trace metal levels in selected fruit juices and carbonated beverages purchased in lagos, nigeria were determined using atomic absorption spectrophotometer (unicam model 969) equipped with solaar 32 windows software. fruit juices analysed were grape, pineapple, apple, orange, lemon juices and their brand names were used. some carbonated drinks were also evaluated for metal levels. trace metals investigated were cr, cu, pb, mn, ni, zn, sn, fe, cd and co. trace metal contents of fruit juices were f ... | 2009 | 18704729 |
study of pineapple peelings processing into vinegar by biotechnology. | this study aimed to reduce post-harvest losses of pineapple local variety egbenana by the transformation of juice into vinegar through biotechnological process. vinegar was produced through two successive fermentations: alcoholic and acetic fermentations. the alcohol fermentation was carried out at 30 degrees c using yeast. biomass, ph and brix were evaluated daily during the fermentation. acetic fermentation was carried out at 30 degrees c using an acetic bacteria strain isolated from pineapple ... | 2009 | 19803120 |
bromelain inhibits cox-2 expression by blocking the activation of mapk regulated nf-kappa b against skin tumor-initiation triggering mitochondrial death pathway. | chemoprevention impels the pursuit for either single targeted or cocktail of multi-targeted agents. bromelain, potential agent in this regard, is a pharmacologically active compound, present in stems and fruits of pineapple (ananas cosmosus), endowed with anti-inflammatory, anti-invasive and anti-metastatic properties. herein, we report the anti tumor-initiating effects of bromelain in 2-stage mouse skin tumorigenesis model. pre-treatment of bromelain resulted in reduction in cumulative number o ... | 2009 | 19339108 |
evaluation of the antioxidant activity of non-transformed and transformed pineapple: a comparative study. | pineapple has several beneficial properties including antioxidant activity. we investigated the antioxidant effect of different extracts of non-transformed (s) and transformed pineapple (with the magainin gene construct, [ts], for disease resistance). they were examined using 1,1-diphenyl-2-picryl hydrazyl (dpph) radical scavenging, oxygen radical absorbance capacity (orac) and lipid peroxidation assays besides phenolic and flavonoid contents. hplc analysis was carried out to identify the possib ... | 2009 | 19563857 |
pilot-scale vermicomposting of pineapple wastes with earthworms native to accra, ghana. | pineapple wastes, an abundant organic waste in accra, ghana, were vermicomposted using native earthworms (eudrilus eugeniae kinberg) collected from the banks of streams and around bath houses of this city. triplicate pilot-scale vermidigesters containing about 90 earthworms and three other control boxes with no earthworms were fed pineapple pulp or peels, and the loss of wet mass was monitored over 20 weeks. in a second experiment, a 1:1 mixture of pineapple peels and pulp (w/w) was fed to tripl ... | 2009 | 19620003 |
effect of bromelain on milk yield, milk composition and mammary health in dairy goats. | a 7 month prospective cohort study was designed to determine if feeding bromelain to dairy goats influenced the mscc, milk yield, milk composition and the incidence of imi. forty-four clinically normal goats from 2nd to 6th parities were studied. daily bromelain dosage was 7.4 grams/animal (185-mg/kg weight). samples for diagnostic bacteriology were collected from each udder half every 2 weeks. samples for mscc and composition were obtained every 42 days. milk yield was also recorded every 42 da ... | 2009 | 18661260 |
bromelain treatment reduces cd25 expression on activated cd4+ t cells in vitro. | bromelain (br), an extract from pineapple stem with cysteine protease activity, exerts anti-inflammatory effects in a number of inflammatory models. we have previously shown that br treatment decreased activated cd4(+) t cells and has a therapeutic role in an ovalbumin-induced murine model of allergic airway disease. the current study was designed to determine the effect of br on cd4(+) t cell activation, specifically the expression of cd25 in vitro. cd25 is up regulated upon t cell activation, ... | 2009 | 19162239 |
genome organization and phylogenetic relationship of pineapple mealybug wilt associated virus-3 with family closteroviridae members. | the nucleotide sequence of pineapple mealybug wilt associated virus-3 (pmwav-3) (closteroviridae: ampelovirus), spanning seven open reading frames (orfs) and the untranslatable region of the 3' end was determined. based on the amino acid identities with orthologous orfs of pmwav-1 (54%-73%) and pmwav-2 (13%-35%), we propose pmwav-3 is a new species in the pmwav complex. pmwav-3 lacks an intergenic region between orf1b and orf2, encodes a relatively small, 28.8 kda, coat protein, and lacks a coat ... | 2009 | 19225875 |
potential for land application of contaminated sewage sludge treated with fermented liquid from pineapple wastes. | the suitability for land application of anaerobically digested sewage sludge treated with naturally fermented and aspergillus niger (a. niger) fermented raw liquid from pineapple wastes, in terms of changes in the forms and amount of heavy metals and nutrient and pathogen content, were investigated in this study. leaching studies for fermented liquid at optimum conditions (ph and contact time with best metal removal efficiencies) were carried out for the removal of cd, cr, cu, pb, ni and zn from ... | 2009 | 19232826 |
high-efficiency agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration. | an improved method for the agrobacterium infiltration of epicotyl segments of 'pineapple' sweet orange [citrus sinensis (l.) osbeck] and 'swingle' citrumelo [citrus paradisi macf. x poncirus trifoliata (l.) raf.] was developed in order to increase transformation frequency. sonication-assisted agrobacterium-mediated transformation (saat), vacuum infiltration, and a combination of the two procedures were compared with conventional agrobacterium-mediated inoculation method ('dipping' method). it wa ... | 2009 | 19048258 |
the comparative study digestion and metabolism of nitrogen and purine derivatives in male, thai, swamp buffalo and thai, brahman cattle. | studies on in vivo digestion, rates of passages, metabolism of nitrogen, urinary purine derivative excretion and blood metabolites were carried out in thai brahman cattle and thai swamp buffaloes (16 months old). the animals were fed mixed diets based on pineapple (ananas comusus) waste silage containing urea-n (npn) and true protein from a concentrate (tp). the brahman cattle (310 +/- 15 kg) were heavier than the swamp buffaloes (195 +/- 9.4 kg) and had higher dry matter (dm), organic matter (o ... | 2009 | 20163582 |
bacteriological evaluation of pre-cut fruits sold in kano metropolis, kano state, nigeria. | one hundred and fifty (150) pre-cut fruit samples comprising of pineapples (50), paw-paw (50) and watermelon (50) at the point of stand retail outlets were tested by standard bacteriological methods to determine bacterial contamination of the fruits. out of these 150 examined 136 (90.67%) were contaminated with bacteria. the bacterial distribution were; escherichia coli 69 (46.00%), staphylococcus aureus 29 (19.33%), salmonella species 13 (8.67%), proteus species 18 (12.00%), enterobacter aeroge ... | 2009 | 20000065 |
[attraction of sphenophorus levis vaurie adults (coleoptera: curculionidae) to vegetal tissues at different conservation levels]. | the occurrence of the sugarcane weevil sphenophorus levis vaurie is important in sugarcane in some regions in brazil. damage is caused by the larvae as they bore into the nodes and can reach 30 ton/ha/year. many control alternatives have been attempted, but none were satisfactory, except for the use of toxic baits. therefore, it is necessary to optimize their efficiency or to propose new techniques. the objective of the current study was to evaluate the attractiveness of adults of s. levis to su ... | 2009 | 20098932 |
[development of mixed drink of coconut water, pineapple and acerola pulp]. | the purpose of the present work was to develop a blended beverage based on green coconut (cocos nucifera l.) water, pineapple (ananas comosus) and acerola (malpighia emarginata d. c.) pulps as a ready to drink product, combining the sensory acceptance, nutritional and functional compounds of these fruits. ten formulations of mixed beverages were evaluated with regard physicochemical determinations, functional compounds and sensorial attributes. since here was no significative change in the accep ... | 2009 | 20677460 |
development of an immunomagnetic capture-reverse transcriptase-pcr assay for three pineapple ampeloviruses. | a semi-automated, immunomagnetic capture-reverse transcription pcr (imc-rt-pcr) assay for the detection of three pineapple-infecting ampeloviruses, pineapple mealybug wilt-associated virus-1, -2 and -3, is described. the assay was equivalent in sensitivity but more rapid than conventional immunocapture rt-pcr. the assay can be used either as a one- or two-step rt-pcr and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf ti ... | 2009 | 18996414 |
inactivation of escherichia coli in a tropical fruit smoothie by a combination of heat and pulsed electric fields. | moderate heat in combination with pulsed electric fields (pef) was investigated as a potential alternative to thermal pasteurization of a tropical fruit smoothie based on pineapple, banana, and coconut milk, inoculated with escherichia coli k12. the smoothie was heated from 25 degrees c to either 45 or 55 degrees c over 60 s and subsequently cooled to 10 degrees c. pef was applied at electric field strengths of 24 and 34 kv/cm with specific energy inputs of 350, 500, and 650 kj/l. both processin ... | 2008 | 19019120 |
antimicrobial activity and potential use of monoterpenes as tropical fruits preservatives. | banana, papaya and pineapple are the most consumed tropical fruits in the world, being brazil one of the main producers. fungi colletotrichum musae, colletotrichum gloeosporioides and fusarium subglutinans f.sp. ananas cause severe post harvest diseases and losses in fruits quality. the aim of this work was to evaluate the effectiveness of five monoterpenes to inhibit the mycelial growth and conidia germination of these three phytopathogens. the monoterpenes citral, citronellal, l-carvone, isopu ... | 2008 | 24031197 |
pomegranate juice inhibits sulfoconjugation in caco-2 human colon carcinoma cells. | several fruit juices have been reported to cause food-drug interactions, mainly affecting cytochrome p450 activity; however, little is known about the effects of fruit juices on conjugation reactions. among several fruit juices tested (apple, peach, orange, pineapple, grapefruit, and pomegranate), pomegranate juice potently inhibited the sulfoconjugation of 1-naphthol in caco-2 cells. this inhibition was both dose- and culture time-dependent, with a 50% inhibitory concentration (ic(50)) value ca ... | 2008 | 19053852 |
the role of enzyme supplementation in digestive disorders. | this article reviews various forms of enzyme supplementation used clinically in digestive and absorption disorders. enzyme supplementation plays an integral role in the management of various digestive disorders, particularly with regard to exocrine pancreatic insufficiency. however, application of enzymes may also be beneficial for other conditions associated with poor digestion including lactose intolerance. historically, porcine and bovine pancreatic enzymes have been the preferred form of sup ... | 2008 | 19152478 |
carotenoid production by xanthophyllomyces dendrorhous: use of pineapple juice as a production medium. | to select a carotenoid-hyperproducing mutant and to formulate pineapple juice as a carotenoid production medium. | 2008 | 18673431 |
[diversity and ichthyofaunistic abundance of the rio grande de térraba, south of costa rica]. | the diversity, abundance and distributional pattern of freshwater fish communities in the térraba river, south costa rica, were investigated from the early dry season of 2004 to early rain season of 2005. there have been no preview studies on the freshwater fish distribution in térraba. therefore, the aims of this study were to determine fish species richness, abundance and distribution there. fish sampling was done using a combination of gears such as gill net, fine mesh net and visual observat ... | 2008 | 19419055 |
removal of heavy metals from contaminated sewage sludge using aspergillus niger fermented raw liquid from pineapple wastes. | the environmental benefits derived from using citric acid in the removal of heavy metals from contaminated sewage sludge have made it promising as an extracting agent in the chemical extraction process. at present, citric acid is produced commercially by fermentation of sucrose using mutant strains of aspergillus niger (a. niger), and chemical synthesis. in recent years, various carbohydrates and wastes (such as pineapple wastes) have been considered experimentally, to produce citric acid by a. ... | 2008 | 17512728 |
bromelain induces cardioprotection against ischemia-reperfusion injury through akt/foxo pathway in rat myocardium. | bromelain (br), a proteolytic enzyme extracted from the stem of the pineapple, is known to possess anti-inflammatory activity and has been shown to reduce blood viscosity, prevent the aggregation of blood platelets, and improve ischemia-reperfusion (i/r) injury in a skeletal muscle model. we investigated the capacity of br to limit myocardial injury in a global i/r model. adult male sprague-dawley rats were divided into two groups: control (pbs) and br at 10 mg/kg in pbs administered via intrape ... | 2008 | 18192224 |
inhibitory effects of fruit juices on cytochrome p450 2c9 activity in vitro. | there is limited information on the effect of fruits on human cytochrome p450 (cyp) 2c9 activity. the objective of this study was to determine the effect of fruit juice on cyp2c9-mediated drug metabolism. nine citrus fruits and eight tropical fruits were chosen. we investigated effects of the fruits on diclofenac 4'-hydroxylation and tolbutamide hydroxylation by human liver microsomes. among the fruits, pineapple juice showed potent inhibition of cyp2c9 activity. the addition of 25 microl (5.0% ... | 2008 | 18256496 |
complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1. | pineapple mealybug wilt-associated virus-1 (pmwav-1; family closteroviridae, genus ampelovirus) belongs to a complex of mealybug-transmissible viruses found in pineapple worldwide. in this study, the complete genome of pmwav-1 was sequenced and found to be 13.1 kb in length, making it the smallest in the family. the genome encoded seven open reading frames (orfs) and was unusual for an ampelovirus due to the lack of an intergenic region between the rdrp and p6 orfs, an orf encoding a relatively ... | 2008 | 18283409 |
just noticeable differences in component concentrations modify the odor quality of a blending mixture. | the odors we perceive are mainly the result of mixtures of odorants that, however, are commonly perceived as single undivided entities; nevertheless, the processes involved remain poorly explored. it has been recently reported that perceptual blending based on configural olfactory processing can cause odorant mixtures to give rise to an emergent odor not present in the components. the present study examined whether specific component proportions are required to elicit an emergent odor. starting ... | 2008 | 18304991 |
oral bromelain attenuates inflammation in an ovalbumin-induced murine model of asthma. | bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models. the purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (ova)-induced murine model of acute allergic airway disease (aad). to establish aad, female c57bl/6j mice were sensitized with intraperitoneal (i.p.) ova/alum and then challenged with ova aerosols for 3 days. mice were gavaged ... | 2008 | 18317550 |
evaluation of selection strategies alternative to nptii in genetic transformation of citrus. | the neomycin phosphotransferase (nptii) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. the present work investigates three different selection alternatives, comparing them to nptii selection in two citrus genotypes, carrizo citrange and pineapple sweet orange. the first method used the beta-glucuronidase (uida) reporter marker gen ... | 2008 | 18317775 |
profiling of different bioactive compounds in functional drinks by high-performance liquid chromatography. | in the present work, an hplc method is proposed to simultaneously detect and quantify water- and fat-soluble vitamins, phenolic compounds, carotenoids and chlorophylls in a single run, by using an ultradeactivated c18 column and gradient separation using trifluoroacetic acid, water and methanol. it is shown that the hplc method provides baseline separation of all these compounds with good resolution values in 40 min. moreover, other figures of merit of the method show a good linear response and ... | 2008 | 18325525 |
cloning, localization and expression analysis of vacuolar sugar transporters in the cam plant ananas comosus (pineapple). | in photosynthetic tissues of the cam plant pineapple (ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal co(2) fixation. however, soluble sugars produced photosynthetically must also be exported to support growth processes in heterotrophic tissues. to begin to address how vacuolar sugar storage and assimilate partitioning are regulated in a. comosus, degenerate pcr and cdna l ... | 2008 | 18408220 |
bacterial cellulose production by acetobacter xylinum strains from agricultural waste products. | bacterial cellulose is a biopolysaccharide produced from the bacteria, acetobacter xylinum. static batch fermentations for bacterial cellulose production were studied in coconut and pineapple juices under 30 degrees c in 5-l fermenters by using three acetobacter strains: a. xylinum tistr 998, a. xylinum tistr 975, and a. xylinum tistr 893. experiments were carried out to compare bacterial cellulose yields along with growth kinetic analysis. results showed that a. xylinum tistr 998 produced a bac ... | 2008 | 18418756 |
inactivation of escherichia coli and listeria innocua in kiwifruit and pineapple juices by high hydrostatic pressure. | escherichia coli and listeria innocua in kiwifruit and pineapple juices were exposed to high hydrostatic pressure (hhp) at 300 mpa for 5 min. both bacteria showed equal resistance to hhp. using low (0 degrees c) or sub-zero (-10 degrees c) temperatures instead of room temperature (20 degrees c) during pressurization did not change the effectiveness of hhp treatment on both bacteria in studied juices. pulse pressure treatment (multiple pulses for a total holding time of 5 min at 300 mpa) instead ... | 2008 | 18455820 |
bromelain treatment decreases neutrophil migration to sites of inflammation. | bromelain, a mixture of proteases derived from pineapple stem, has been reported to have therapeutic benefits in a variety of inflammatory diseases, including murine inflammatory bowel disease. the purpose of this work was to understand potential mechanisms for this anti-inflammatory activity. exposure to bromelain in vitro has been shown to remove a number of cell surface molecules that are vital to leukocyte trafficking, including cd128a/cxcr1 and cd128b/cxcr2 that serve as receptors for the n ... | 2008 | 18482869 |