Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| biodegradation of imidacloprid by consortium of two soil isolated bacillus sp. | imidacloprid degradation potential of bacterial cultures from sugarcane growing soils was studied in liquid culture and bacillus aerophilus and bacillus alkalinitrilicus showed maximum potential to degrade imidacloprid. hence, into a clay loam soil imidacloprid was added at 50, 100, and 150 mg kg(-1) along with 45 × 10(7) cells g(-1) soil of both species under autoclaved and unautoclaved conditions. under autoclaved conditions imidacloprid residues were degraded after 56 days to 3.18, 5.83 and 1 ... | 2014 | 25257222 |
| isolation and characterization of novel phorate-degrading bacterial species from agricultural soil. | based upon 16s rdna sequence homology, 15 phorate-degrading bacteria isolated from sugarcane field soils by selective enrichment were identified to be different species of bacillus, pseudomonas, brevibacterium, and staphylococcus. relative phorate degradation in a mineral salt medium containing phorate (50 μg ml(-1)) as sole carbon source established that all the bacterial species could actively degrade more than 97 % phorate during 21 days. three of these species viz. bacillus aerophilus strain ... | 2014 | 24046230 |
| the sugarcane defense protein sugarwin2 causes cell death in colletotrichum falcatum but not in non-pathogenic fungi. | plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. two homologues of a barley wound-inducible protein (barwin) have been characterized in sugarcane, sugarwin1 and sugarwin2 (sugarcane wound-inducible proteins). induction of sugarwins occurs in response to diatraea saccharalis damage but not to pathogen infection. in addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activitie ... | 2014 | 24608349 |
| a novel thermostable xylanase gh10 from malbranchea pulchella expressed in aspergillus nidulans with potential applications in biotechnology. | the search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. the present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (gh10) from the thermophilic fungus malbranchea pulchella, the influence of glycosylation on its stability, and a potential application in sugarcane bagasse hydrolysis. | 2014 | 25788980 |
| liquefaction of sugarcane bagasse for enzyme production. | the objective of this paper is to report liquefaction of pretreated and sterilized sugarcane bagasse for enhancing endoglucanase production through submerged fermentation by aspergillus niger. after initial solid state fermentation of steam pretreated bagasse solids by a. niger, fed-batch addition of the substrate to cellulase in buffer over a 12h period, followed by 36h reaction, resulted in a liquid slurry with a viscosity of 0.30±0.07pas at 30% (w/v) solids. addition of a. niger for submerged ... | 2014 | 25265329 |
| utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using aspergillus niger gh1. | agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. invertase is an important enzyme for sweeteners development. in this study, a xerophilic fungus aspergillus niger gh1 isolated of the mexican semi-desert, previously reported as an invertase over-producer strain was used. molasses from mexico and cuba were chemical ... | 2014 | 25242918 |
| synergistic effect of aspergillus niger and trichoderma reesei enzyme sets on the saccharification of wheat straw and sugarcane bagasse. | plant-degrading enzymes can be produced by fungi on abundantly available low-cost plant biomass. however, enzymes sets after growth on complex substrates need to be better understood, especially with emphasis on differences between fungal species and the influence of inhibitory compounds in plant substrates, such as monosaccharides. in this study, aspergillus niger and trichoderma reesei were evaluated for the production of enzyme sets after growth on two "second generation" substrates: wheat st ... | 2014 | 25116172 |
| use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation. | the enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals. here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (scb) used for cellulase production was investigated by means of spectroscopic and imaging techniques. chemical composition and structural characteristics, such as crystallinity (determined by ... | 2014 | 24363237 |
| association between aflatoxin b1 occupational airway exposure and risk of hepatocellular carcinoma: a case-control study. | the aim of this study was to determine the airway exposure of sugar and papermaking factory workers to aflatoxin b1 (afb1) and to explore the potential association between afb1 airway exposure and the risk of hepatocellular carcinoma (hcc) in a case-control study. dust samples were collected from the sugarcane bagasse warehouse, and presser and paper production workshops. blood samples were collected from 181 workshop employees and 203 controls who worked outside the workshop. afb1 albumin adduc ... | 2014 | 24961349 |
| identification and characterisation of xylanolytic yeasts isolated from decaying wood and sugarcane bagasse in brazil. | in this study, yeasts associated with lignocellulosic materials in brazil, including decaying wood and sugarcane bagasse, were isolated, and their ability to produce xylanolytic enzymes was investigated. a total of 358 yeast isolates were obtained, with 198 strains isolated from decaying wood and 160 strains isolated from decaying sugarcane bagasse samples. seventy-five isolates possessed xylanase activity in solid medium and were identified as belonging to nine species: candida intermedia, c. t ... | 2014 | 24748334 |
| application of rna interference methodology to investigate and develop scmv resistance in maize. | specific fragments of the sugarcane mosaic virus (scmv) coat protein gene (cp) were amplified by reverse transcriptionpolymerase chain reaction and used to construct a marker free small interfering rna complex expression vector against scmv. in planta transformation was performed on maize (zea mays) inbred line 8112 mediated by agrobacterium tumefaciens. pcr and southern blot analyses demonstrated successful integration of the cp segment into the 8112 genome. the in planta transformation frequen ... | 2014 | 25189224 |
| physiological and photosynthetic characteristics of indica hang2 expressing the sugarcane pepc gene. | phosphoenolpyruvate carboxylase (pepc) is known to play a key role in the initial fixation of co2 in c4 photosynthesis. the pepc gene from sugarcane (a c4 plant) was introduced into indica rice (hang2), a process mediated by agrobacterium tumefaciens. integration patterns and copy numbers of the gene was confirmed by dna blot analysis. rt-pcr and western blotting results showed that the pepc gene was expressed at both the mrna and protein levels in the transgenic lines. real-time pcr results ind ... | 2014 | 24469712 |
| field performance of transgenic sugarcane produced using agrobacterium and biolistics methods. | future genetic improvement of sugarcane depends, in part, on the ability to produce high-yielding transgenic cultivars with improved traits such as herbicide and insect resistance. here, transgenic sugarcane plants generated by different transformation methods were assessed for field performance over 3 years. agrobacterium-mediated (agro) transgenic events (35) were produced using four different agrobacterium tumefaciens strains, while biolistic (biol) transgenic events (48) were produced using ... | 2014 | 24330327 |
| phosphate enhances levan production in the endophytic bacterium gluconacetobacter diazotrophicus pal5. | gluconacetobacter diazotrophicus is a gram-negative and endophytic nitrogen-fixing bacterium that has several beneficial effects in host plants; thus, utilization of this bacterium as a biofertilizer in agriculture may be possible. g. diazotrophicus synthesizes levan, a d-fructofuranosyl polymer with β-(2→6) linkages, as an exopolysaccharide and the synthesized levan improves the stress tolerance of the bacterium. in this study, we found that phosphate enhances levan production by g. diazotrophi ... | 2014 | 24717418 |
| complete genome sequence of kosakonia sacchari type strain sp1(t.). | kosakonia sacchari sp. nov. is a new species within the new genus kosakonia, which was included in the genus enterobacter. k sacchari is a nitrogen-fixing bacterium named for its association with sugarcane (saccharum officinarum l.). k sacchari bacteria are gram-negative, aerobic, non-spore-forming, motile rods. strain sp1(t) (=cgmcc1.12102(t)=lmg 26783(t)) is the type strain of the k sacchari sp. nov and is able to colonize and fix n2 in association with sugarcane plants, thus promoting plant g ... | 2014 | 25197499 |
| insecticide-degrading burkholderia symbionts of the stinkbug naturally occupy various environments of sugarcane fields in a southeast island of japan. | the stinkbug cavelerius saccharivorus, which harbors burkholderia species capable of degrading the organophosphorus insecticide, fenitrothion, has been identified on a japanese island in farmers' sugarcane fields that have been exposed to fenitrothion. a clearer understanding of the ecology of the symbiotic fenitrothion degraders of burkholderia species in a free-living environment is vital for advancing our knowledge on the establishment of degrader-stinkbug symbiosis. in the present study, we ... | 2014 | 25736865 |
| a fine-scale phylogenetic analysis of free-living burkholderia species in sugarcane field soil. | the diversity and abundance of burkholderia species in sugarcane field soils were investigated by a 16s rrna gene-based approach using genus-specific primers. a total of 365,721 sequences generated by the illumina miseq platform were assigned to the genus burkholderia. nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. quantitative pcr analysis revealed a consistent number of 16s rrna gene copies for burkholderia species (10(7) g(-1) soil) ac ... | 2014 | 25410730 |
| evidence of environmental and vertical transmission of burkholderia symbionts in the oriental chinch bug, cavelerius saccharivorus (heteroptera: blissidae). | the vertical transmission of symbiotic microorganisms is omnipresent in insects, while the evolutionary process remains totally unclear. the oriental chinch bug, cavelerius saccharivorus (heteroptera: blissidae), is a serious sugarcane pest, in which symbiotic bacteria densely populate the lumen of the numerous tubule-like midgut crypts that the chinch bug develops. cloning and sequence analyses of the 16s rrna genes revealed that the crypts were dominated by a specific group of bacteria belongi ... | 2014 | 25038101 |
| isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of china and optimization of cellulase production by paenibacillus terrae me27-1. | from different natural reserves in the subtropical region of china, a total of 245 aerobic bacterial strains were isolated on agar plates containing sugarcane bagasse pulp as the sole carbon source. of the 245 strains, 22 showed hydrolyzing zones on agar plates containing carboxymethyl cellulose after congo-red staining. molecular identification showed that the 22 strains belonged to 10 different genera, with the burkholderia genus exhibiting the highest strain diversity and accounting for 36.36 ... | 2014 | 25050355 |
| inhibition of sucrose phosphatase by sucrose. | 1. partially purified sucrose phosphatase from immature stem tissue of sugarcane is inhibited by sucrose. the enzyme was also inhibited by maltose, melezitose and 6-kestose but not by eight other sugars, including glucose and fructose. 2. the relative effectiveness of sucrose, maltose and melezitose as inhibitors is different for sucrose phosphatase from different plants. 3. the inhibition of the sugar-cane enzyme by sucrose was shown to be partially competitive. the k(i) for sucrose is about 10 ... | 2014 | 4291490 |
| drought tolerance conferred to sugarcane by association with gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways. | sugarcane interacts with particular types of beneficial nitrogen-fixing bacteria that provide fixed-nitrogen and plant growth hormones to host plants, promoting an increase in plant biomass. other benefits, as enhanced tolerance to abiotic stresses have been reported to some diazotrophs. here we aim to study the effects of the association between the diazotroph gluconacetobacter diazotrophicus pal5 and sugarcane cv. sp70-1143 during water depletion by characterizing differential transcriptome pr ... | 2014 | 25489849 |
| genomic analysis of xanthomonas translucens pathogenic on wheat and barley reveals cross-kingdom gene transfer events and diverse protein delivery systems. | in comparison to dicot-infecting bacteria, only limited numbers of genome sequences are available for monocot-infecting and in particular cereal-infecting bacteria. herein we report the characterisation and genome sequence of xanthomonas translucens isolate dar61454 pathogenic on wheat and barley. based on phylogenetic analysis of the atp synthase beta subunit (atpd) gene, dar61454 is most closely related to other x. translucens strains and the sugarcane- and banana- infecting xanthomonas strain ... | 2014 | 24416331 |
| optimization of pretreatment and fermentation conditions for production of extracellular cellulase complex using sugarcane bagasse. | sugarcane bagasse (scb), a lignocellulosic byproduct of juice extraction from sugarcane, is rich in cellulose (40-42%). this could be used as a substrate for the production of cellulase complex. fermentation conditions were optimized for production of cellulase complex (cmcase, cellulobiase and fpase) by wild type trichoderma sp. using sugarcane bagasse as sole carbon source. alkaline treatment (2% naoh) of bagasse (alscb) was found suitable for the production of reducing sugar over the acidic p ... | 2014 | 25489168 |
| genome sequencing of xanthomonas vasicola pathovar vasculorum reveals variation in plasmids and genes encoding lipopolysaccharide synthesis, type-iv pilus and type-iii secretion effectors. | xanthomonas vasicola pathovar vasculorum (xvv) is the bacterial agent causing gumming disease in sugarcane. here, we compare complete genome sequences for five isolates of xvv originating from sugarcane and one from maize. this identified two distinct types of lipopolysaccharide synthesis gene clusters among xvv isolates: one is similar to that of xanthomonas axonopodis pathovar citri (xac) and is probably the ancestral type, while the other is similar to those of the sugarcane-inhabiting specie ... | 2014 | 25437615 |
| production of bioethanol using agricultural waste: banana pseudo stem. | india is amongst the largest banana (musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. two fungal strains aspergillus ellipticus and aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse wer ... | 2014 | 25477922 |
| optimization of key factors affecting hydrogen production from sugarcane bagasse by a thermophilic anaerobic pure culture. | hydrogen is regarded as an attractive future energy carrier for its high energy content and zero co2 emission. currently, the majority of hydrogen is generated from fossil fuels. however, from an environmental perspective, sustainable hydrogen production from low-cost lignocellulosic biomass should be considered. thermophilic hydrogen production is attractive, since it can potentially convert a variety of biomass-based substrates into hydrogen at high yields. | 2014 | 25184001 |
| bioethanol production from fermentable sugar juice. | bioethanol production from renewable sources to be used in transportation is now an increasing demand worldwide due to continuous depletion of fossil fuels, economic and political crises, and growing concern on environmental safety. mainly, three types of raw materials, that is, sugar juice, starchy crops, and lignocellulosic materials, are being used for this purpose. this paper will investigate ethanol production from free sugar containing juices obtained from some energy crops such as sugarca ... | 2014 | 24715820 |
| finished genome of zymomonas mobilis subsp. mobilis strain cp4, an applied ethanol producer. | zymomonas mobilis subsp. mobilis is one of the most rigorous ethanol-producing organisms known to date, considered by many to be the prokaryotic alternative to yeast. the two most applied z. mobilis subsp. mobilis strains, zm4 and cp4, derive from recife, brazil, and have been isolated from sugarcane fermentations. of these, zm4 was the first z. mobilis representative strain to be sequenced and analyzed. here, we report the finishing of the genome sequence of strain cp4, which is highly similar ... | 2014 | 24407627 |
| spatial variability of soil potassium in sugarcane areas subjected to the application of vinasse. | when deposited on land the vinasse can promote improvement in fertility, however, often fertilizer application occurs in areas considered homogeneous, without taking into account the variability of the soil. the objective of this study was to evaluate the effect of vinasse application on potassium content in two classes of soils cultivated with sugarcane, and characterize the spatial variability of soil using geostatistical techniques. in the 2010 and 2011 crop year, soil samples were collected ... | 2014 | 25590735 |
| effect of land use on mayfly assemblages structure in neotropical headwater streams. | the aim of this study was to test the effect of agricultural and forestry land use on the structure of mayfly assemblages in low-order streams. twenty-nine headwater streams were investigated in the state of são paulo. we analyzed 15 streams in pristine areas (mixed tropical rainforest, semideciduous forest and dense tropical rainforest), and 14 streams covered with sugarcane, eucalyptus and pasture. mayfly richness obtained by rarefaction curves was higher in pristine areas (21 genera), especia ... | 2014 | 25590712 |
| silicon reduces impact of plant nitrogen in promoting stalk borer (eldana saccharina) but not sugarcane thrips (fulmekiola serrata) infestations in sugarcane. | the stalk borer eldana saccharina walker (lepidoptera: pyralidae) is a major limiting factor in south african sugarcane production, while yield is also reduced by sugarcane thrips fulmekiola serrata kobus (thysanoptera: thripidae). borer management options include appropriate nitrogen (n) and enhanced silicon (si) nutrition; the effect of n on sugarcane thrips is unknown. we tested the effects of these nutrients, in combination with resistant (n33) and susceptible (n27) sugarcane cultivars, on e ... | 2014 | 24999349 |
| geographic population structure of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), in the southern united states. | the sugarcane borer moth, diatraea saccharalis, is widespread throughout the western hemisphere, and is considered an introduced species in the southern united states. although this moth has a wide distribution and is a pest of many crop plants including sugarcane, corn, sorghum and rice, it is considered one species. the objective was to investigate whether more than one introduction of d. saccharalis had occurred in the southern united states and whether any cryptic species were present. we fi ... | 2014 | 25337705 |
| exchanging physically effective neutral detergent fiber does not affect chewing activity and performance of late-lactation dairy cows fed corn and sugarcane silages. | the objective of this study was to determine whether replacing the physically effective neutral detergent fiber (pendf) of corn silage with sugarcane silage pendf would affect performance in dairy cows. twenty-four late-lactation holstein cows were assigned to eight 3 × 3 latin squares with 21-d periods. the dietary treatments were (1) 25% pendf of corn silage, (2) 25% pendf of sugarcane silage, and (3) 12.5% pendf of corn silage + 12.5% pendf of sugarcane silage. the physical effectiveness fact ... | 2014 | 25242426 |
| digesta sampling sites and marker methods for estimation of ruminal outflow in bulls fed different proportions of corn silage or sugarcane. | the objective of this experiment was to assess ruminal outflow and apparent total-tract digestibility using digesta samples from 3 sites (reticulum, omasum, and abomasum) and 3 marker methods (single marker: indigestible ndf [indf; sample without separation]; double marker: indf + co-edta [filtered sample]; and triple marker: indf + ytterbium [yb] acetate + co-edta [filtered and centrifuged]) in bulls fed corn silage and sugarcane-based diets. eight crossbred (holstein × zebu) bulls (353 ± 37 kg ... | 2014 | 24778340 |
| the impact of dietary sugarcane addition to finishing diets on performance, apparent digestibility, and fatty acid composition of holstein × zebu bulls. | the objective of this study was to evaluate the influence of corn silage (cs) or sugarcane (sc) additions to finishing diets on voluntary intake, apparent digestibility, animal performance, and lm fatty acid (fa) composition in crossbred holstein × zebu bulls. forty-two bulls (initial bw 328 ± 39.2 kg and an initial age of 23.5 mo) were used in this experiment. a completely randomized design was used to examine roughage source (cs vs. sc) and ratio of concentrate (co) on nutrient utilization and ... | 2014 | 24713164 |
| critical electrolyte concentration of silk gland chromatin of the sugarcane borer diatraea saccharalis, induced using agrochemicals. | the sugarcane borer diatraea saccharalis is widely known as the main pest of sugarcane crop, causing increased damage to the entire fields. measures to control this pest involve the use of chemicals and biological control with cotesia flavipes wasps. in this study, we evaluated the insecticides fipronil (frontline; 0.0025%), malathion (malatol bio carb; 0.4%), cipermetrina (galgotrin; 10%), and neem oil (natuneem; 100%) and the herbicide nicosulfuron (sanson 40 sc; 100%) in the posterior region ... | 2014 | 25299111 |
| new species and new records of tetranychidae (acarina, prostigmata) from thailand. | sampling efforts conducted in several provinces from thailand disclosed three new species of tetranychid mites. two of them belong to the genus tetranychus, namely tetranychus occultaspina sp. nov. and tetranychus truncatissimus sp. nov. and the third species belongs to the genus schizotetranychus, schizotetranychus krungthepensis sp. nov. they were collected on ipomoea aquatica, bambusa multiplex and saccharum officinarum, respectively. new records and new hosts are also mentioned. | 2014 | 24871006 |
| abiotic limits for germination of sugarcane seed in relation to environmental spread. | sugarcane is a vegetatively propagated crop and hence the production of seed and its fate in the environment has not been studied. the recent development of genetically modified sugarcane, with the aim of commercial production, requires a research effort to understand sugarcane reproductive biology. this study contributes to this understanding by defining the abiotic limits for sugarcane seed germination. using seed from multiple genetic crosses, germination was measured under different light re ... | 2014 | 25485029 |
| using exploratory factor analysis of ffq data to identify dietary patterns among yup'ik people. | an ffq developed by the center for alaska native health research for studies in yup'ik people includes market foods and subsistence foods such as moose, seal, waterfowl and salmon that may be related to disease risk. because the ffq contains >100 food items, we sought to characterize dietary patterns more simply for use in ongoing pharmacogenomics studies. | 2014 | 23290469 |
| effects of food processing and fibre content on the digestibility, energy intake and biochemical parameters of blue-and-gold macaws (ara ararauna l. - aves, psittacidae). | considering the increased incidence of obesity and metabolic diseases in caged psittacines, the effect of fibre and food processing was evaluated in the blue-and-gold macaw. four food formulations (0%, 7%, 14% and 21% of sugarcane fibre) processed by pelleting or extrusion were studied, resulting in eight diets. to study digestibility, 48 macaws housed in pairs in cages was used in a block design. subsequently, diets containing 0% or 21% sugarcane fibre, pelleted or extrude was fed for 4 months ... | 2014 | 23627662 |
| reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes. | polyhydroxyalkanoates (phas) are bacterial carbon storage polymers used as renewable, biodegradable plastics. pha production in plants may be a way to reduce industrial pha production costs. we recently demonstrated a promising level of peroxisomal pha production in the high biomass crop species sugarcane. however, further production strategies are needed to boost pha accumulation closer to commercial targets. through exogenous fatty acid feeding of arabidopsis thaliana plants that contain perox ... | 2014 | 24944109 |
| induced over-expression of atdreb2a ca improves drought tolerance in sugarcane. | drought is one of the most challenging agricultural issues limiting sustainable sugarcane production and, in some cases, yield losses caused by drought are nearly 50%. dreb proteins play vital regulatory roles in abiotic stress responses in plants. the transcription factor dreb2a interacts with a cis-acting dre sequence to activate the expression of downstream genes that are involved in drought-, salt- and heat-stress response in arabidopsis thaliana. in the present study, we evaluated the effec ... | 2014 | 24656336 |
| genetic diversity among puccinia melanocephala isolates from brazil assessed using simple sequence repeat markers. | brown rust (causal agent puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. despite its importance, little is known regarding the genetic diversity of this pathogen in the main brazilian sugarcane cultivation areas. in this study, we characterized the genetic diversity of 34 p. melanocephala isolates from 4 brazilian states using loci identified from an enriched simple sequence repeat (ssr) library. the aggressiveness of 3 isolates ... | 2014 | 25299099 |
| pyraclostrobin wash-off from sugarcane leaves and aerobic dissipation in agricultural soil. | to mitigate damage from the fungal sugarcane pathogen brown rust (puccinia melanocephala), a section 18 emergency use label was put in place by the united states environmental protection agency (u.s. epa) for the application of pyraclostrobin (trade name headline sc, produced by basf, research triangle park, nc) on sugarcane in 2008. to assess the dynamics of this fungicide in louisiana soil, samples (n = 24) from a non-treated field were spiked with pyraclostrobin (3.1 μg g(-1)) and analyzed in ... | 2014 | 24533668 |
| utilization of agricultural residues of pineapple peels and sugarcane bagasse as cost-saving raw materials in scenedesmus acutus for lipid accumulation and biodiesel production. | the aim of this study is to optimize the lipid accumulation in microalgae by using two agricultural residues of pineapple peels and sugarcane bagasse as low-cost organic carbon sources. green microalgae scenedesmus acutus was isolated and selected for cultivation. effects of three initial sugar concentrations and the stage for adding sugar during cultivation on biomass and lipid production were investigated. the results clearly showed that two-stage cultivation is more suitable than one-stage. t ... | 2014 | 24817554 |
| short and long-term antinutritional effect of the trypsin inhibitor apti for biological control of sugarcane borer. | plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. in this work, we tested the anti-insect activity of adenanthera pavonina trypsin inhibitor (apti) against diatraea saccharalis larvae, a major insect pest in sugarcane. the addition of 0.1% apti in short-term assays resulted in 87% and 63% decreased trypsin and chymotrypsin activities respectively. apti was not digested after 60h incubation with ... | 2014 | 24355140 |
| [control discourses and power relations of yellow fever: philadelphia in 1793]. | 1793 yellow fever in philadelphia was the most severe epidemics in the late 18th century in the united states. more than 10% of the population in the city died and many people fled to other cities. the cause of yellow fever in the united states had close relationship with slaves and sugar in philadelphia. sugarcane plantation had needed many labors to produce sugar and lots of africans had to move to america as slaves. in this process, aëdes aegypti, the vector of yellow fever had migrated to am ... | 2014 | 25608507 |
| defense response in non-genomic model species: methyl jasmonate exposure reveals the passion fruit leaves' ability to assemble a cocktail of functionally diversified kunitz-type trypsin inhibitors and recruit two of them against papain. | multiplicity of protease inhibitors induced by predators may increase the understanding of a plant's intelligent behavior toward environmental challenges. information about defense mechanisms of non-genomic model plant passion fruit (passiflora edulis sims) in response to predator attack is still limited. here, via biochemical approaches, we showed its flexibility to build-up a broad repertoire of potent kunitz-type trypsin inhibitors (ktis) in response to methyl jasmonate. seven inhibitors (20- ... | 2014 | 24849173 |
| breaking dogmas: the plant vascular pathogen xanthomonas albilineans is able to invade non-vascular tissues despite its reduced genome. | xanthomonas albilineans, the causal agent of sugarcane leaf scald, is missing the hrp type iii secretion system that is used by many gram-negative bacteria to colonize their host. until now, this pathogen was considered as strictly limited to the xylem of sugarcane. we used confocal laser scanning microscopy, immunocytochemistry and transmission electron microscopy (tem) to investigate the localization of x. albilineans in diseased sugarcane. sugarcane plants were inoculated with strains of the ... | 2014 | 24522883 |
| process optimization and production kinetics for cellulase production by trichoderma viride vkf3. | microbial cellulases are the enzymes widely studied due to their enormous applications in biochemical industry. among 12 fungal isolates isolated from mangrove plant debris and soil sample collected from valanthakad mangroves, kerala, india, 3 of them were found to exhibit cellulolytic activity. among them, the most potent isolate which exhibited maximum cellulolytic activity was identified as trichoderma viride vkf3 [gene bank accession number- jx683684.1] based on colony morphology, microscopi ... | 2014 | 24600546 |
| molecular profiling of systemic acquired resistance (sar)-responsive transcripts in sugarcane challenged with colletotrichum falcatum. | red rot disease of sugarcane caused by colletotrichum falcatum is one of the serious constraints affecting the productivity of the crop. the strategy of employing systemic acquired resistance (sar) against red rot yielded consistently good results at field level. however, elucidation of genes involved in the induction of sar continues to be a challenging area of research for a critical understanding of red rot disease resistance in sugarcane. here, temporal expression of 22 putative defense-rela ... | 2014 | 25234394 |
| [surveillance experience in the sugarcane sector: challenges in disrupting the perilous "marathon" of the sugarcane plantations]. | the sugar-alcohol sector is growing year by year, especially in the state of sao paulo where approximately 42.9% of the sugar-ethanol plants are concentrated. the production chain is a subject for concern to public agencies and to civil society by exposing migrant workers to risks arising from the work process. in sao paulo, from 2006-2009, occupational health surveillance (visat) set up two initiatives to address problems related to the housing and working conditions of sugarcane workers. the o ... | 2014 | 25388174 |
| genetic diversity and coefficient of parentage between clones and sugarcane varieties in brazil. | the success of the development of new sugarcane varieties is associated with the ability to correctly select the genitor. the aim of this study was to evaluate the genetic diversity between 113 clones and sugarcane varieties using the ward-modified location model procedure with added information about the coefficient of parentage and endogamy. in this study, data was used from 100 experiments that evaluated clones; the experimental phase was conducted in 70 places between the years 2002 and 2009 ... | 2014 | 25366791 |
| nuclear magnetic resonance investigation of water accessibility in cellulose of pretreated sugarcane bagasse. | enzymatic hydrolysis is a crucial step of biomass conversion into biofuels and different pretreatments have been proposed to improve the process efficiency. amongst the various factors affecting hydrolysis yields of biomass samples, porosity and water accessibility stand out due to their intimate relation with enzymes accessibility to the cellulose and hemicellulose fractions of the biomass. in this work, sugarcane bagasse was subjected to acid and alkali pretreatments. the changes in the total ... | 2014 | 25342969 |
| resistance of sugarcane cultivars to mahanarva fimbriolata (stål) (hemiptera: cercopidae). | the spittlebug mahanarva fimbriolata (stål) (hemiptera: cercopidae) is one of the most important pests of the sugarcane crop in brazil. despite of its importance, there is currently a lack of information regarding sugarcane cultivars' resistance to the spittlebug. therefore, our objective was to evaluate the response of sugarcane genotypes to this species. two experiments were carried out under laboratory conditions using a random block design with treatments in a factorial arrangement of 2 × 13 ... | 2014 | 27193410 |
| valorization of sugarcane bagasse ash: producing glass-ceramic materials. | some aluminosilicates, for example mullite and wollastonite, are very important in the ceramic and construction industries. the most significant glass-ceramic for building applications has wollastonite as the main crystal phase. in this work we report on the use of sugarcane bagasse ash (scba) to produce glass-ceramics with silicates as the major crystalline phases. the glasses (frits) were prepared by mixing ash, limestone (calcium and magnesium carbonates) and potassium carbonate as the fluxin ... | 2014 | 24463731 |
| evaluating the composition and processing potential of novel sources of brazilian biomass for sustainable biorenewables production. | the search for promising and renewable sources of carbohydrates for the production of biofuels and other biorenewables has been stimulated by an increase in global energy demand in the face of growing concern over greenhouse gas emissions and fuel security. in particular, interest has focused on non-food lignocellulosic biomass as a potential source of abundant and sustainable feedstock for biorefineries. here we investigate the potential of three brazilian grasses (panicum maximum, pennisetum p ... | 2014 | 24438499 |
| draft genome sequence of atrazine-utilizing bacteria isolated from indian agricultural soil. | we report the draft genome sequences of two tropical bacterial isolates capable of degrading the herbicide atrazine. alcaligenes sp. strain egd-ak7 and arthrobacter sp. strain ak-yn10 were isolated from indian agricultural soil in which sugarcane is grown, with a reported history of atrazine use. egd-ak7 has the atzabcdef genes and ak-yn10 has the trzn and atzbc genes for atrazine degradation. | 2014 | 24407646 |
| sugarcane fibre may prevents hairball formation in cats. | hair ingested by licking during cat grooming can eventually coalesce into solid masses in cat gastrointestinal tract. it is believed that dietary fibre might reduce formation of these trichobezoars (hairballs). the effects of two insoluble fibre sources added to kibble diets were evaluated with respect to trichobezoar faecal excretion. thirty-two cats and four diets were used in a randomised block design: a control diet without additional fibre, 10 % added sugarcane fibre, 20 % added sugarcane f ... | 2014 | 26101589 |
| a new species of burkholderia isolated from sugarcane roots promotes plant growth. | sugarcane is a globally important food, biofuel and biomaterials crop. high nitrogen (n) fertilizer rates aimed at increasing yield often result in environmental damage because of excess and inefficient application. inoculation with diazotrophic bacteria is an attractive option for reducing n fertilizer needs. however, the efficacy of bacterial inoculants is variable, and their effective formulation remains a knowledge frontier. here, we take a new approach to investigating diazotrophic bacteria ... | 2014 | 24350979 |
| water stress reveals differential antioxidant responses of tolerant and non-tolerant sugarcane genotypes. | the biochemical responses of the enzymatic antioxidant system of a drought-tolerant cultivar (iacsp 94-2094) and a commercial cultivar in brazil (iacsp 95-5000) grown under two levels of soil water restriction (70% and 30% soil available water content) were investigated. iacsp 94-2094 exhibited one additional active superoxide dismutase (cu/zn-sod vi) isoenzyme in comparison to iacsp 95-5000, possibly contributing to the heightened response of iacsp 94-2094 to the induced stress. the total gluta ... | 2014 | 24308986 |
| fungal pretreatment improves amenability of lignocellulosic material for its saccharification to sugars. | the sugarcane bagasse was biologically pretreated with three white-rot fungi; pleurotus florida, coriolopsis caperata rck 2011 and ganoderma sp. rckk-02, individually under solid-state fermentation. p. florida, c. caperata rck 2011 and ganoderma sp. rckk-02 degraded lignin up to 7.91, 5.48 and 5.58%, respectively. the lignocellulolytic enzymes produced by these fungi were also monitored during solid state fermentation of sugarcane bagasse. the fungal fermented sugarcane bagasse when hydrolyzed w ... | 2014 | 24274505 |
| oligomerization, membrane association, and in vivo phosphorylation of sugarcane udp-glucose pyrophosphorylase. | sugarcane is a monocot plant that accumulates sucrose to levels of up to 50% of dry weight in the stalk. the mechanisms that are involved in sucrose accumulation in sugarcane are not well understood, and little is known with regard to factors that control the extent of sucrose storage in the stalks. udp-glucose pyrophosphorylase (ugpase; ec 2.7.7.9) is an enzyme that produces udp-glucose, a key precursor for sucrose metabolism and cell wall biosynthesis. the objective of this work was to gain in ... | 2014 | 25320091 |
| effect of land use on mayfly assemblages structure in neotropical headwater streams. | the aim of this study was to test the effect of agricultural and forestry land use on the structure of mayfly assemblages in low-order streams. twenty-nine headwater streams were investigated in the state of são paulo. we analyzed 15 streams in pristine areas (mixed tropical rainforest, semideciduous forest and dense tropical rainforest), and 14 streams covered with sugarcane, eucalyptus and pasture. mayfly richness obtained by rarefaction curves was higher in pristine areas (21 genera), especia ... | 2014 | 25317730 |
| formulation of trichoderma sp. sl2 inoculants using different carriers for soil treatment in rice seedling growth. | trichoderma sp. sl2 has been previously reported to enhance rice germination, vigour, growth and physiological characteristics. the use of potato dextrose agar as carrier of trichoderma sp. sl2 inoculant is not practical for field application due to its short shelf life and high cost. this study focuses on the use of corn and sugarcane bagasse as potential carriers for trichoderma sp. sl2 inoculants. | 2014 | 25279323 |
| sugarcane biomass for biorefineries: comparative composition of carbohydrate and non-carbohydrate components of bagasse and straw. | two fractions of sugarcane, namely bagasse and straw (or trash), were characterized in relation to their chemical composition. bagasse presented values of glucans, hemicelluloses, lignin and ash of 37.74, 27.23, 20.57 and 6.53%, respectively, while straw had 33.77, 27.38, 21.28 and 6.23% of these same components. ash content was relatively high in both cane biomass fractions. bagasse showed higher levels of contaminating oxides while straw had a higher content of alkaline and alkaline-earth oxid ... | 2014 | 25263869 |
| full-length enriched cdna libraries and orfeome analysis of sugarcane hybrid and ancestor genotypes. | sugarcane is a major crop used for food and bioenergy production. modern cultivars are hybrids derived from crosses between saccharum officinarum and saccharum spontaneum. hybrid cultivars combine favorable characteristics from ancestral species and contain a genome that is highly polyploid and aneuploid, containing 100-130 chromosomes. these complex genomes represent a huge challenge for molecular studies and for the development of biotechnological tools that can facilitate sugarcane improvemen ... | 2014 | 25222706 |
| a fluorescence-based method for cyanate analysis in ethanol/water media: correlation between cyanate presence and ethyl carbamate formation in sugar cane spirit. | based on the fluorescence properties of 2,4-(1h,3h)-quinazolinedione, a product of the reaction between cyanate and 2-aminobenzoic acid, a simple, sensitive, selective, and reproducible method for the cyanate analysis in aqueous ethanolic media is proposed. in this method, λ(exc) and λ(em) are 310 and 410 nm, respectively, and the limits of detection and quantification are 2.2 × 10(-7) and 6.7 × 10(-7) mol/l, respectively. under optimal conditions (ph = 4.5, 40% ethanol), a concentration of 5.0 ... | 2014 | 25220490 |
| factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass. | polyhydroxyalkanoates are linear biodegradable polyesters produced by bacteria as a carbon store and used to produce a range of bioplastics. widespread polyhydroxyalkanoate production in c4 crops would decrease petroleum dependency by producing a renewable supply of biodegradable plastics along with residual biomass that could be converted into biofuels or energy. increasing yields to commercial levels in biomass crops however remains a challenge. previously, lower accumulation levels of the sho ... | 2014 | 25209261 |
| efficient open fermentative production of polymer-grade l-lactate from sugarcane bagasse hydrolysate by thermotolerant bacillus sp. strain p38. | lactic acid is one of the top 30 potential building-block chemicals from biomass, of which the most extensive use is in the polymerization of lactic acid to poly-lactic-acid (pla). to reduce the cost of pla, the search for cheap raw materials and low-cost process for lactic acid production is highly desired. in this study, the final titer of produced l-lactic acid reached a concentration of 185 g·l(-1) with a volumetric productivity of 1.93 g·l(-1)·h(-1) by using sugarcane bagasse hydrolysate as ... | 2014 | 25192451 |
| addition of feruloyl esterase and xylanase produced on-site improves sugarcane bagasse hydrolysis. | accessory enzymes that assist biomass degradation could be used to improve the recovery of fermentable sugar for use in biorefineries. in this study, different fungal strains isolated from the amazon rainforest were evaluated in terms of their ability to produce feruloyl esterase (fae) and xylanase enzymes, and an assessment was made of the contributions of the enzymes in the hydrolysis of pretreated sugarcane bagasse. in the selection step, screening using plate assays was followed by shake fla ... | 2014 | 25151076 |
| species-specific detection and identification of fusarium species complex, the causal agent of sugarcane pokkah boeng in china. | pokkah boeng disease caused by the fusarium species complex results in significant yield losses in sugarcane. thus, the rapid and accurate detection and identification of the pathogen is urgently required to manage and prevent the spreading of sugarcane pokkah boeng. | 2014 | 25141192 |
| development of hemicellulolytic enzyme mixtures for plant biomass deconstruction on target biotechnological applications. | an essential step in the conversion of lignocellulosic biomass to ethanol and other biorefinery products is conversion of cell wall polysaccharides into fermentable sugars by enzymatic hydrolysis. the objective of the present study was to understand the mode of action of hemicellulolytic enzyme mixtures for pretreated sugarcane bagasse (psb) deconstruction and wheat arabinoxylan (wa) hydrolysis on target biotechnological applications. in this study, five hemicellulolytic enzymes-two endo-1,4-xyl ... | 2014 | 25077777 |
| polyhydroxyalkanoate biosynthesis and simultaneous remotion of organic inhibitors from sugarcane bagasse hydrolysate by burkholderia sp. | burkholderia sp. f24, originally isolated from soil, was capable of growth on xylose and removed organic inhibitors present in a hemicellulosic hydrolysate and simultaneously produced poly-3-hydroxybutyrate (p3hb). using non-detoxified hydrolysate, burkholderia sp. f24 reached a cell dry weight (cdw) of 6.8 g l(-1), containing 48 % of p3hb and exhibited a volumetric productivity (pp3hb) of 0.10 g l(-1) h(-1). poly-3-hydroxybutyrate-co-3-hydroxyvalerate copolymers (p3hb-co-3hv) were produced usin ... | 2014 | 25059637 |
| implications of stillage land disposal: a critical review on the impacts of fertigation. | stillage is the main wastewater from ethanol production, generated specifically in the step of distillation. regardless the feedstock, stillage contains high concentrations of organic matter, potassium and sulfates, as well as acidic and corrosive characteristics. currently almost the entire volume of stillage generated in brazilian distilleries is directed to the fertigation of sugarcane fields, due to its fertilizer character. however, the polluting potential of stillage characterizes its land ... | 2014 | 25058869 |
| perspectives on the production of polyhydroxyalkanoates in biorefineries associated with the production of sugar and ethanol. | polyhydroxyalkanoates (pha) are biodegradable and biocompatible bacterial thermoplastic polymers that can be obtained from renewable resources. the high impact of the carbon source in the final cost of this polymer has been one of the major limiting factors for pha production and agricultural residues, mainly lignocellulosic materials, have gained attention to overcome this problem. in brazil, production of 2nd generation ethanol from the glucose fraction, derived from sugarcane bagasse hydrolys ... | 2014 | 25043132 |
| structural and physicochemical characteristics of starch from sugar cane and sweet sorghum stalks. | the starch present in sugar cane and sorghum juice has been considered a problem to the sugar industry. the objective of this work was to study the structural and physicochemical characteristics of the starch present in sugar cane and sweet sorghum. sugar cane and sweet sorghum starches presented small granules (maximum 5.9 and 7.9 μm), a-type diffraction pattern, high degree of relative crystallinity (44.4 and 42.0%), and low amylose content (17.5 and 16.4%), respectively. sugar cane starch pre ... | 2014 | 25037392 |
| validation of novel reference genes for reverse transcription quantitative real-time pcr in drought-stressed sugarcane. | one of the most challenging aspects of rt-qpcr data analysis is the identification of reliable reference genes. ideally, they should be neither induced nor repressed under different experimental conditions. to date, few reference genes have been adequately studied for sugarcane (saccharum spp.) using statistical approaches. in this work, six candidate genes ( αtub, gapdh, h1, samdc, ubq, and 25s rrna) were tested for gene expression normalization of sugarcane root tissues from drought-tolerant a ... | 2014 | 24987730 |
| building the sugarcane genome for biotechnology and identifying evolutionary trends. | sugarcane is the source of sugar in all tropical and subtropical countries and is becoming increasingly important for bio-based fuels. however, its large (10 gb), polyploid, complex genome has hindered genome based breeding efforts. here we release the largest and most diverse set of sugarcane genome sequences to date, as part of an on-going initiative to provide a sugarcane genomic information resource, with the ultimate goal of producing a gold standard genome. | 2014 | 24984568 |
| effect of chemical factors on integrated fungal fermentation of sugarcane bagasse for ethanol production by a white-rot fungus, phlebia sp. mg-60. | bioethanol production through integrated fungal fermentation (iff), involving a unified process for biological delignification with consolidated biological processing by the white-rot fungus phlebia sp. mg-60, was applied to sugarcane bagasse. initial moisture content of the bagasse was found to affect biological delignification by mg-60, and 75% moisture content was suitable for selective lignin degradation and subsequent ethanol production. additives, such as basal media, organic compounds, or ... | 2014 | 24968109 |
| the impact of stress on the health of sugar cane cutters. | evaluate the impact of stress on sugar cane cutters and the prevalence of physical and psychological symptoms before and after harvest. | 2014 | 24897043 |
| description of helicotylenchus persiaensis sp. n. (nematoda: hoplolaimidae) from iran . | in order to identify the species of helicotylenchus steiner, 1945 present in iran, 497 soil and root samples were collected from the rhizosphere of different plants and localities throughout the country during 2009-2010. a new and several known species of helicotylenchus were identified from the collected material. h. persiaensis sp. n. is characterized by its short tail (8-11 µm , c = 54.2-79.0, c' = 0.6-1.2), usually with smooth terminus or with 1-3 very coarse annules, rarely with minor ventr ... | 2014 | 24872247 |
| biogeographical variation and population genetic structure of sporisorium scitamineum in mainland china: insights from issr and sp-srap markers. | a total of 100 sporisorium scitamineum isolates were investigated by inter simple sequence repeat (issr) and single primer-sequence related amplified polymorphism (sp-srap) markers. these isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis or by principal component analysis (pca) of the issr, sp-srap, or issr + sp-srap data set. the total gene diversity (h t) and gene diversity between subpopulations (h s) were estimated to be 0.34 to 0. ... | 2014 | 24772015 |
| selfing rate estimation in sugarcane under unfavorable natural conditions of crossing by using microsatellite markers. | the self-fertilization or selfing rate estimation using microsatellite markers and its impact on survival and selection rate were evaluated in families derived from polycrosses that involved parents that were widely used in sugarcane breeding in brazil. these factors were evaluated under unfavorable natural conditions of flowering and crossing. after the germination test, the viable progeny were taken to the field for survival rate evaluation (4, 6, and 10 months) and phenotypic selection at pla ... | 2014 | 24737476 |
| paternity identification in sugarcane polycrosses by using microsatellite markers. | although polycrosses have been used to test the potential of cross-combination of a large number of sugarcane parents, the male parent of the half-sib progenies produced is unknown. the present study aimed to integrate the molecular marker technology to the sugarcane polycross approach by the application of microsatellite markers to identify the male parent of 41 elite clones derived from polycross families. ten microsatellite [single sequence repeats (ssrs)] primer pairs were used to identify t ... | 2014 | 24737475 |
| genetic variability among the chloroplast genomes of sugarcane (saccharum spp) and its wild progenitor species saccharum spontaneum l. | a striking characteristic of modern sugarcane is that all sugarcane cultivars (saccharum spp) share a common cytoplasm from s. officinarum. to explore the potential value of s. spontaneum cytoplasm, new saccharum hybrids with an s. spontaneum cytoplasm were developed at the united states department of agriculture-agricultural research service, sugarcane research laboratory, through a combination of conventional and molecular breeding approaches. in this study, we analyzed the genetic variability ... | 2014 | 24615073 |
| using multibiomarker approach as a tool to improve the management plan for a private reserve of natural heritage (rppn). | this study aimed to monitor an aquatic ecosystem during two different periods (dry and rainy season) on a protected area located inside a sugarcane farm, using the fish astyanax sp. as bioindicator. an integrated approach was adopted by combining the responses of well-known biomarkers: acetylcholinesterase, lipid peroxidation (lpo), catalase (cat), glutathione s-transferase (gst), micronucleus test, and liver histopathology. the activity of enzymes cat and gst was increased after the rainy seaso ... | 2014 | 24531322 |
| structural and functional characterization of the chaperone hsp70 from sugarcane. insights into conformational changes during cycling from cross-linking/mass spectrometry assays. | hsp70 cycles from an atp-bound state, in which the affinity for unfolded polypeptides is low, to an adp-bound state, in which the affinity for unfolded polypeptides is high, to assist with cell proteostasis. such cycling also depends on co-chaperones because these proteins control both the hsp70 atpase activity and the delivery of unfolded polypeptide chains. although it is very important, structural information on the entire protein is still scarce. this work describes the first cloning of a cd ... | 2014 | 24530624 |
| assessment of in vitro cyto/genotoxicity of sequentially treated electroplating effluent on the human hepatocarcinoma huh-7 cell line. | the present study compares in vitro toxicity of electroplating effluent after the batch treatment process with that obtained after the sequential treatment process. activated charcoal prepared from sugarcane bagasse through chemical carbonization, and tolerant indigenous bacteria, bacillus sp. strain ist105, were used individually and sequentially for the treatment of electroplating effluent. the sequential treatment involving activated charcoal followed by bacterial treatment removed 99% of cr( ... | 2014 | 24525376 |
| de novo assembly and transcriptome analysis of contrasting sugarcane varieties. | sugarcane is an important crop and a major source of sugar and alcohol. in this study, we performed de novo assembly and transcriptome annotation for six sugarcane genotypes involved in bi-parental crosses. the de novo assembly of the sugarcane transcriptome was performed using short reads generated using the illumina rna-seq platform. we produced more than 400 million reads, which were assembled into 72,269 unigenes. based on a similarity search, the unigenes showed significant similarity to mo ... | 2014 | 24523899 |
| early changes in arbuscular mycorrhiza development in sugarcane under two harvest management systems. | sugarcane (saccharum spp.) is grown on over 8 million ha in brazil and is used to produce ethanol and sugar. some sugarcane fields are burned to facilitate harvesting, which can affect the soil microbial community. however, whether sugarcane pre-harvest burning affects the community of arbuscular mycorrhizal fungi (amf) and symbioses development is not known. in this study, we investigated the early impacts of harvest management on amf spore communities and root colonization in three sugarcane v ... | 2014 | 25477936 |
| a method to estimate the biomass of spirulina platensis cultivated on a solid medium. | this paper presents a method to estimate the biomass of spirulina cultivated on solid medium with sugarcane bagasse as a support, in view of the difficulty in determining biomass concentrations in bioprocesses, particularly those conducted in semi-solid or solid media. the genus spirulina of the family oscillatoriaceae comprises the group of multicellular filamentous cyanobacteria (blue-green microalgae). spirulina is used as fish feed in aquaculture, as a food supplement, a source of vitamins, ... | 2014 | 25477928 |
| field performance of sugarcane (saccharum sp.) plants derived from cryopreserved calluses. | this study compared the field performance of sugarcane plants originating from three different sources: control, non-cryopreserved embryogenic calluses, cryopreserved embryogenic calluses and macropropagated material of the same commercial hybrid. several agronomic traits were evaluated on 100 plants per treatment over a 27-month period covering the growth of the stool and of the first ratoon. significant differences between treatments were observed only during the first six months of field grow ... | 2014 | 11912504 |
| antioxidant and nitrite-scavenging capacities of phenolic compounds from sugarcane (saccharum officinarum l.) tops. | sugarcane tops were extracted with 50% ethanol and fractionated by petroleum ether, ethyl acetate (etoac), and n-butyl alcohol successively. eight phenolic compounds in etoac extracts were purified through silica gel and sephadex lh-20 column chromatographies, and then identified by nuclear magnetic resonance and electrospray ionization mass spectra. the results showed that eight phenolic compounds from etoac extracts were identified as caffeic acid, cis-p-hydroxycinnamic acid, quercetin, apigen ... | 2014 | 25162956 |
| variation in chilling tolerance for photosynthesis and leaf extension growth among genotypes related to the c4 grass miscanthus ×giganteus. | the goal of this study was to identify cold-tolerant genotypes within two species of miscanthus related to the exceptionally chilling-tolerant c4 biomass crop accession: m. ×giganteus 'illinois' (mxg) as well as in other mxg genotypes. the ratio of leaf elongation at 10 °c/5 °c to that at 25 °c/25 °c was used to identify initially the 13 most promising miscanthus genotypes out of 51 studied. net leaf co2 uptake (a sat) and the maximum operating efficiency of photosystem ii (фpsii) were measured ... | 2014 | 25039073 |
| comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-pcr. | the increasingly used real time quantitative reverse transcription-pcr (qrt-pcr) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s). in order to facilitate gene expression studies in sugarcane (saccharum officinarum), a non-model plant with limited genome information, the stability of 13 candidate reference genes was evaluated. the genorm, normfinder and deltact methods were used for selecting stably expressed internal controls across ... | 2014 | 24823940 |
| extraction optimization by response surface methodology: purification and characterization of phytosterol from sugarcane (saccharum officinarum l.) rind. | a green, simple, and effective method for the extraction of sugarcane lipids from sugarcane rind was investigated by response surface methodology. the optimum conditions of technological progress obtained through response surface methodology were as follows: liquid-to-solid ratio 7.94: 1 ml/g, extraction temperature 50°c and extraction time 5.98 h. the practical sugarcane lipids extraction yield was 6.55 ± 0.28%, which was in good consistence with the predicted extraction yield of 6.47%. the res ... | 2014 | 24648272 |
| a comprehensive genetic map of sugarcane that provides enhanced map coverage and integrates high-throughput diversity array technology (dart) markers. | sugarcane genetic mapping has lagged behind other crops due to its complex autopolyploid genome structure. modern sugarcane cultivars have from 110-120 chromosomes and are in general interspecific hybrids between two species with different basic chromosome numbers: saccharum officinarum (2n = 80) with a basic chromosome number of 10 and s. spontaneum (2n = 40-128) with a basic chromosome number of 8. the first maps that were constructed utilised the single dose (sd) markers generated using rflp, ... | 2014 | 24564784 |
| genetic diversity in sugarcane varieties in brazil based on the ward-modified location model clustering strategy. | we evaluated the genetic diversity of 77 clones of sugarcane used in crosses made by the brazilian interuniversity network for the development of the sugar/energy sector (ridesa) breeding program. characterization of the genotypes was carried out at the ratoon stage, based on eight morphological traits and seven agronomic traits. diversity analysis was carried out beginning with the ward-modified location model. the ideal number of groups was three. groups 1, 2, and 3 were composed of 37, 21, an ... | 2014 | 24535897 |
| phytochemical contents and antioxidant capacities of different parts of two sugarcane (saccharum officinarum l.) cultivars. | antioxidant capacities and phytochemicals such as phenolics, flavonoids, triterpenoids, phytosterols of four parts of two sugarcane cultivars have been characterised. the total triterpenoid content (ttc), total phenolic content (tpc), total flavonoid content (tfc) and total sterol content (tsc) were different among parts and cultivars of sugarcane. the node of both cultivars contained the highest ttc (2096.02 and 1779.66 mg ursolic acid/100g dw for green-rind and red-rind sugarcane, respectively ... | 2014 | 24423556 |