Publications

molecular characterization and expression studies during melon fruit development and ripening of l-galactono-1,4-lactone dehydrogenase.	the last step of ascorbic acid (aa) biosynthesis is catalysed by the enzyme l-galactono-1,4-lactone dehydrogenase (galldh, ec 1.3.2.3), located on the inner mitochondrial membrane. the enzyme converts l-galactono-1,4-lactone to ascorbic acid (aa). in this work, the cloning and characterization of a galldh full-length cdna from melon (cucumis melo l.) are described. melon genomic dna southern analysis indicated that cmgalldh was encoded by a single gene. cmgalldh mrna accumulation was detected in ...	2004	15234991
cloning of genes differentially expressed during the initial stage of fruit development in melon (cucumis melo cv. reticulatus).	we have cloned genes involved in the initial stage of fruit development in the melon by suppression subtractive hybridization. a cdna library of unfertilized ovules was subtracted from that of fruit 9 days after pollination (dap); 10 of the 40 selected cdna clones were identified by reverse northern analysis as genes differentially expressed in fruit at 9 dap. seven of the ten genes were homologous to genes of known function; two were related to genes with unknown functions, and one was novel. w ...	2004	15179036
promotion of 5-aminolevulinic acid on photosynthesis of melon (cucumis melo) seedlings under low light and chilling stress conditions.	when melon seedlings (cucumis melo l. ximiya no. 1) were cultured in a growth chamber with about 150 micro mol m(-2) s(-1) photon flux density, the leaf photosynthetic ability reduced dramatically as leaf position decreased from the top. the application of 5-aminolevulinic acid (ala) solutions significantly increased the net photosynthetic rate (p(n)) as well as apparent quantum yield (aqy), carboxylation efficiency (ce) and stomata conductance (g(s)). after irrigation with 10 ml of ala solution ...	2004	15153193
development of microsatellite markers from an enriched genomic library for genetic analysis of melon (cucumis melo l.).	despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon (cucumis melo l.) and cucurbit species. the development of microsatellite markers will have a major impact on genetic analysis and breeding of melon, especially on the generation of marker saturated genetic maps and implementation of marker assisted breeding programs. genomic microsatellite enriched libraries can be an eff ...	2004	15149552
peroxidase activity associated with suberization processes of the muskmelon (cucumis melo) rind.	the rind of fruits of muskmelon (cucumis melo l. var. reticulatus) contains a network of suberized tissue referred to as the 'netting', and peroxidase (ec 1.11.1.7) activity is necessary to the polymerization of the aromatic domain of suberin. peroxidase activity increased dramatically during the early stages of melon fruit netting, and in fruits exhibiting incomplete netting, peroxidase activity was significantly higher in netted than in non-netted regions of the same fruit. moreover, analysis ...	2004	15086828
pre-extraction preparation (fresh, frozen, freeze-dried, or acetone powdered) and long-term storage of fruit and vegetable tissues: effects on antioxidant enzyme activity.	activities of the antioxidant enzymes ascorbate peroxidase, catalase, dehydroascorbate reductase, glutathione reductase, guaiacol peroxidase, monodehydroascorbate reductase, and superoxide dismutase were assayed in honeydew (cucumis melo l.) fruit and spinach (spinacia oleracea l.) leaves either as fresh, frozen to -80 degrees c, frozen in liquid nitrogen, freeze-dried, or acetone powder, representing the various ways tissues are treated prior to enzyme extraction. treated tissues were analyzed ...	2004	15080616
single-nucleotide polymorphisms detected in expressed sequence tags of melon (cucumis melo l.).	a search was performed for single-nucleotide polymorphisms (snp) and short insertions-deletions (indels) in 34 melon (cucumis melo l.) expressed sequence tag (est) fragments between two distantly related melon genotypes, a group inodorus 'piel de sapo' market class breeding line t111 and the korean accession pi 161375. in total, we studied 15 kb of melon sequence. the average frequency of snps between the two genotypes was one every 441 bp. one indel was also found every 1666 bp. seventy-five pe ...	2004	15060588
identification of 1,8-dihydroxynaphthalene melanin in monosporascus cannonballus and the analysis of hexaketide and pentaketide compounds produced by wild-type and pigmented isolates of the fungus.	monosporascus cannonballus causes root rot and vine decline in muskmelons and watermelons. wild types of this fungus often undergo degenerative changes that have been associated with yellow to brown pigmentation, hypovirulence, dsrna infection, and decreased production of perithecia. in this study, degenerate isolates that produced yellow to brown pigments and no perithecia were obtained from wild-type cultures that had been stored for extended periods of time. cultures of the degenerate isolate ...	2004	15212456
isolation and identification of hexaketides from a pigmented monosporascus cannonballus isolate.	monosporascus cannonballus causes severe production losses to muskmelon and watermelon in the united states and other countries. wild types of the fungus produce no pigments when grown on potato dextrose agar (pda). after long-term storage on soil/oat hull mix, however, some isolates of the fungus produce yellow to brown pigments and no perithecia when grown on pda. five colored metabolites from pigmented cultures of m. cannonballus isolate tx923038 have now been identified. two of these, monosp ...	2004	15212455
linkage map of cucumis melo including phenotypic traits and sequence-characterized genes.	a new linkage map of cucumis melo, derived from the f2 progeny of a cross between pi 414723 and c. melo 'topmark' is presented. the map spans a total of 1421 cm and includes 179 points consisting of random amplified polymorphic dna (rapd), amplified fragment length polymorphism (aflp), inter-simple sequence repeats (issrs), simple sequence repeats (ssrs), and restriction fragment length polymorphism (rflp) markers. the map also includes an aphid resistance trait (vat) and the sex type gene, andr ...	2003	14608393
clinical characteristics of melon (cucumis melo) allergy.	although melon is a frequent allergy-eliciting fruit, allergic reactions to melon have rarely been reported.	2003	14533664
genetic analysis of spanish melon ( cucumis melo l.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions.	genetic relationships among 125 spanish melon ( cucumis melo l.) accessions from a spanish germplasm collection were assessed using a standard molecular-marker array consisting of 34 random amplified polymorphic dna (rapd) markers bands (19 primers) and 72 reference accessions drawn from previous studies. the reference accession array consisted of a broad range [japanese (19) crete (17), african (15), and usa and europe (us/eu, 21)] of horticultural groupings (group cantalupensis, group conomon, ...	2003	14504742
cloning and functional expression of alkaline alpha-galactosidase from melon fruit: similarity to plant sip proteins uncovers a novel family of plant glycosyl hydrolases.	raffinose and stachyose are ubiquitous galactosyl-sucrose oligosaccharides in the plant kingdom which play major roles, second only to sucrose, in photoassimilate translocation and seed carbohydrate storage. these sugars are initially metabolised by alpha-galactosidases (alpha-gal). we report the cloning and functional expression of the first genes, cmaga1 and cmaga2, encoding for plant alpha-gals with alkaline ph optima from melon fruit (cucumis melo l.), a raffinose and stachyose translocating ...	2003	12943544
production of haploid and doubled haploid plants of melon ( cucumis melo l) for use in breeding for multiple virus resistance.	we have developed improved procedures for recovery of haploid and doubled haploid (dh) melon plants, using hybrids derived from crosses of lines with multiple virus resistance. seeds formed after pollination with irradiated pollen were cultured in liquid medium for 10 days before excision of the embryos for further culture. this made it easier to identify the seeds containing parthenogenetic embryos, thereby reducing the effort required and increasing the percentage of plants recovered. the plan ...	2003	12836007
identification of cucumisin (cuc m 1), a subtilisin-like endopeptidase, as the major allergen of melon fruit.	allergenic components in melon extracts have not been described in spite of the fact that melon (cucumis melo) is a frequent allergy-eliciting fruit. the aim of this study was to evaluate allergenic components in melon extract and to report the identification of cucumisin as a major melon allergen.	2003	12801320
sensory and quality analysis of different melon cultivars after prolonged storage.	the purpose of this work was to evaluate the sensory and general quality of four different melon cultivars (cucumis melo l.) immediately after harvest and at the end of storage and marketing simulation. after 16 days of storage at 5 degrees c and additional 3 days at 20 degrees c, only cultivar 'c-8' had a poor general appearance due to significant low firmness and relatively high decay incidence compared to the cultivars '5080', 'ideal' and '7302'. the cultivar '7302' was found to have the high ...	2003	14609087
nucleotide sequences of two korean isolates of cucumber green mottle mosaic virus.	the nucleotide sequences of the genomic rnas of cucumber green mottle mosaic virus korean watermelon isolate (cgmmv-kw) and korean oriental melon isolate (cgmmv-kom) were determined and compared to the sequences of other tobamoviruses including cgmmv strains w and sh. each cgmmv isolate had a genome of 6,424 nucleotides. each also had 60 and 176 nucleotides of 5' and 3' untranslated regions (utrs), respectively, and four open reading frames (orf1-4). orfs 1 to 4 encode proteins of 129, 186, 29, ...	2003	14744034
functional and phylogenetic analyses of a conserved regulatory program in the phloem of minor veins.	the minor-vein phloem of mature leaves is developmentally and physiologically distinct from the phloem in the rest of the vascular system. phloem loading of transport sugars occurs in the minor veins, and consistent with this, galactinol synthase is expressed in the minor veins of melon (cucumis melo) as part of the symplastic-loading mechanism that operates in this species. a galactinol synthase promoter from melon drives gene expression in the minor-vein companion cells of both transgenic toba ...	2003	14526110
recovery of surface bacteria from and surface sanitization of cantaloupes.	practical, effective methods that could be implemented in a food service establishment (restaurant or delicatessen) for the surface sanitization of cantaloupes were microbiologically evaluated. cantaloupes (cucumis melo l. var. reticulates) were immersed in an inoculum containing salmonella enterica serovar poona or pantoea agglomerans at ca. 10(4) to 10(5) cfu/ml. an efficient method for the recovery of bacteria from the cantaloupe surface was developed and validated. the method consisted of wa ...	2003	14572217
melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation.	abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ...	2003	18943165
powdery mildew (sphaerotheca fuliginea) resistance in melon is selectable at the haploid level.	the major cause of powdery mildew in melons (cucumis melo l.) is the fungus sphaerotheca fuliginea. there are several cultivar- and season-specific races of this fungus. in order to control powdery mildew, it is important to introduce resistance to fungal infection into new cultivars during melon breeding. haploid breeding is a powerful tool for the production of pure lines. in this study, it was investigated whether powdery mildew resistance could be manifested at the haploid level from two dis ...	2003	12598576
binary transformation systems based on 'shooter' mutants of agrobacterium tumefaciens: a simple, efficient and universal gene transfer technology that permits marker gene elimination.	a simple transformation procedure with a positive selection scheme using the expression of the isopentenyl transferase ( ipt) gene of transfer dna (t-dna) 'shooter' mutants of agrobacterium tumefaciens was elaborated. after comparing several 'shooter' mutants we found that particular strains frequently produced phenotypically normal shoots after co-culturing with tobacco leaf explants. shoots selected for normal phenotype showed apical dominance and could be rooted with the same efficiency as no ...	2003	12789522
aflp analysis of a worldwide collection of didymella bryoniae.	didymella bryoniae (anamorph phoma cucurbitacearum) is an ascomycete that causes gummy stem blight, a foliar disease that occurs on cucurbits in greenhouses and fields throughout the world. in a previous study using rapd analysis, little genetic diversity was found among isolates of d. bryoniae from new york and south carolina, usa. here we report the use of amplified fragment length polymorphism (aflp) analysis to assess the genetic variation within a worldwide collection of d. bryoniae, 102 fi ...	2003	12825498
on-farm and postharvest processing sources of bacterial contamination to melon rinds.	multistate and international foodborne illness outbreaks, particularly involving cantaloupe and often involving rare salmonella spp., have increased dramatically over the past 13 years. this study assessed the sources and extent of melon rind contamination in production fields and at processing and packing facilities. in the spring of 1999, cantaloupe (cucumis melo l. [reticulatus group] cv. cruiser) sampled from two sites in the rio grande river valley showed that postharvest-processed melon ri ...	2003	12540185
biocontrol of listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin.	the fresh-cut produce industry has been the fastest-growing portion of the food retail market during the past 10 years, providing consumers with convenient and nutritious food. however, fresh-cut fruits and vegetables raise food safety concerns, because exposed tissue may be colonized more easily by pathogenic bacteria than intact produce. this is due to the higher availability of nutrients on cut surfaces and the greater potential for contamination because of the increased amount of handling. w ...	2003	12902237
identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed bac library. microcolinearity between cucumis melo and arabidopsis thaliana.	a bacterial artificial chromosome (bac) library from the dihaploid melon line 'pit92' was constructed with a 6 times coverage of the haploid melon genome. a contig of four bacs around the mrgh63 resistance gene homologue fragment was created. the complete sequence of a 117-kb bac clone allowed to determine two clearly defined regions, the first one containing a cluster of three resistance gene homologues. separated by a retrotransposon, that contains large long terminal repeats, the second regio ...	2003	12678558
ozone increases root respiration but decreases leaf co2 assimilation in cotton and melon.	it is well established that exposure of plant foliage to tropospheric ozone (o3) inhibits photosynthetic gas exchange in leaves and the translocation of current photosynthate to sink tissues. it is less clear what impact o3-reduced source strength has on the physiological responses of sink tissue such as fine roots. the responses were investigated of carbon acquisition in leaves and carbon utilization in the respiration of fine roots, following chronic (weeks) and acute (hours) exposures to o3 i ...	2003	12947052
evaluation of aroma compounds contributing to muskmelon flavor in porapak q extracts by aroma extract dilution analysis.	the flavor of the miyabi variety of japanese muskmelon was extracted according to the porapak q column method (pqm) and evaluated by using aroma extract dilution analysis (aeda) method. the overall odor of the pqm extracts was perceived as having a natural muskmelon-like odor, suggesting that the pqm was able to extract volatile compounds in muskmelon fruit without degradation of original flavor. forty-six odorant compounds [kovats index (ki), 961 < or = ki < or = 2605] were found by gc-sniffing ...	2003	12744676
effect of storage on some volatile aroma compounds in fresh-cut cantaloupe melon.	changes in volatile aroma constituents of fresh-cut cantaloupe melon with storage were determined by headspace solid-phase microextraction gas chromatography-mass spectrometry. the compounds isolated from the fruit immediately after cutting were predominantly aliphatic and aromatic esters. storage of fruit at 4 degrees c caused a considerable decrease in concentration of esters and synthesis of the terpenoid compounds beta-ionone and geranylacetone over a period of 24 h. this change in the volat ...	2002	12083880
role of ethylene in the biosynthetic pathway of aliphatic ester aroma volatiles in charentais cantaloupe melons.	compared to other melon types, cantaloupe charentais melons are highly aromatic with a major contribution to the aroma being made by aliphatic and branched esters. using a transgenic line in which the synthesis of the plant hormone ethylene has been considerably lowered by antisense acc oxidase mrna (as), the aliphatic ester pathway steps at which ethylene exerts its regulatory role were found. the data show that the production of aliphatic esters such as hexyl and butyl acetate was blocked in a ...	2002	11807123
carvacrol and cinnamic acid inhibit microbial growth in fresh-cut melon and kiwifruit at 4 degrees and 8 degrees c.	to establish whether or not carvacrol and cinnamic acid delay microbial spoilage of fresh-cut fruit.	2002	12390487
relationship between crop losses and initial population densities of meloidogyne arenaria in winter-grown oriental melon in korea.	to determine the economic threshold level, oriental melon (cucumis melo l. cv. geumssaragi-euncheon) grafted on shintozoa (cucurbita maxima x cu. moschata) was planted in plots (2 x 3 m) under a plastic film in february with a range of initial population densities (pi) of meloidogyne arenaria. the relationships of early, late, and total yield to pi measured in september and january were adequately described by both linear regression and the seinhorst damage model. initial nematode densities in s ...	2002	19265907
resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance.	genomic and cdna fragments with homology to known disease resistance genes (rgh fragments) were cloned from cucumis melo using degenerate-primer pcr. fifteen homologues of the nbs-lrr gene family have been isolated. the nbs-lrr homologues show high divergence and, based on the partial nbs-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a tir-protein element and one that lacks such a domain. genomic organizati ...	2002	12582612
molecular and genetic characterization of a non-climacteric phenotype in melon reveals two loci conferring altered ethylene response in fruit.	fruit ripening and abscission are associated with an ethylene burst in several melon (cucumis melo) genotypes. in cantaloupe as in other climacteric fruit, exogenous ethylene can prematurely induce abscission, ethylene production, and ripening. melon genotypes without fruit abscission or without ethylene burst also exist and are, therefore, non-climacteric. in the nonabscising melon fruit pi 161375, exogenous ethylene failed to stimulate abscission, loss of firmness, ethylene production, and exp ...	2002	12011360
isolation and identification of a phosphate deficiency-induced c-glycosylflavonoid that stimulates arbuscular mycorrhiza formation in melon roots.	melon (cucumis melo) roots were inoculated with or without the arbuscular mycorrhizal (am) fungus glomus caledonium under low phosphate conditions. high-performance liquid chromatography analysis of the secondary metabolites in butanol extracts from roots revealed that the level of one compound in noninoculated roots showed a significant increase from 30 days postinoculation. no accumulation was observed in mycorrhizal roots and high-phosphate-supplemented roots, indicating that the accumulation ...	2002	12026171
relationship of cell surface charge and hydrophobicity to strength of attachment of bacteria to cantaloupe rind.	the cantaloupe melon has been associated with outbreaks of salmonella infections. it is suspected that bacterial surface charge and hydrophobicity may affect bacterial attachment and complicate bacterial detachment from cantaloupe surfaces. the surface charge and hydrophobicity of strains of salmonella, escherichia coli (o157:h7 and non-o157:h7), and listeria monocytogenes were determined by electrostatic and hydrophobic interaction chromatography, respectively. initial bacterial attachment to c ...	2002	12117240
sphingomonas melonis sp. nov., a novel pathogen that causes brown spots on yellow spanish melon fruits.	a polyphasic taxonomic study was performed on the phytopathogenic bacterial strains dapp-pg 224(t) and dapp-pg 228, which cause brown spot on yellow spanish melon (cucumis melo var. inodorus) fruits. based on the presence of glucuronosyl ceramide (sgl-1) in cellular lipids, the results of fatty acid analysis and 16s rdna sequence comparison, the strains had been identified as belonging to the genus sphingomonas and as phylogenetically related to sphingomonas mali, sphingomonas pruni and sphingom ...	2002	12508872
isolation of nonpathogenic mutants of fusarium oxysporum f. sp. melonis for biological control of fusarium wilt in cucurbits.	abstract two nonpathogenic mutant strains 4/4 and 15/15 of fusarium oxysporum f. sp. melonis (race 1,2) were isolated by a continuous dipinoculation technique following uv mutagenesis of the virulent wild-type isolate fom1.2. no disease symptoms or detrimental effects were observed following inoculation of muskmelon seedlings by strain 4/4. in contrast, strain 15/15 caused mortality of susceptible cultivars although to a lesser extent than the wild-type isolate. strain 4/4 colonized a variety of ...	2002	18943089
internal transcribed spacer regions 1 and 2 and random amplified polymorphic dna analysis of didymella bryoniae and related phoma species isolated from cucurbits.	abstract didymella bryoniae (anamorph phoma cucurbitacearum) is the causal agent of gummy stem blight, although other phoma species are often isolated from cucurbit plants exhibiting symptoms of the disease. the molecular and phylogenetic relationships between d. bryoniae and these phoma species are unknown. isolates of d. bryoniae and phoma obtained from cucurbits grown at various geographical locations in the united states were subjected to random amplified polymorphic dna (rapd) analysis and ...	2002	18944025
detection of 3-hydroxy-3-methylglutaryl-coenzyme a reductase protein cm-hmgr during fruit development in melon ( cucumis melo l.).	the fruit size of melon ( cucumis melo l. reticulatus) is determined by the amount of cell proliferation in the pericarp during early fruit development. during this stage, expression and activity of the 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr) gene is required for fruit growth. in this study, we performed a detailed analysis of the correlation between the expression of melon hmgr (cm-hmgr) protein and cell division in the pericarp. flow cytometric analysis revealed that the length ...	2002	12582637
a reference map of cucumis melo based on two recombinant inbred line populations.	a composite genetic melon map was generated based on two recombinant inbred line (ri) populations. by analyzing the segregation of 346 aflps, 113 imas and phenotypic characters on a ri population of 163 individuals derived from the cross védrantais x pi 161375, a first map was constructed. about 20% of the molecular markers were skewed, and the residual heterozygosity was estimated at 4.43% which was not significantly different from the theoretical value of 4.2%. the genome distribution of molec ...	2002	12582608
genetics of ethylene biosynthesis and restriction fragment length polymorphisms (rflps) of acc oxidase and synthase genes in melon ( cucumis melo l.).	we investigated the genetics of ethylene biosynthesis and its linkage to the rflps of the acc oxidase and synthase genes in melon ( cucumis melo l.). the results suggested that the a(0) and b(0) fragments of rflp-mel1 of the acc oxidase gene were two alleles from a single locus, as were the b and c fragments of rflp-meacs1 of the acc synthase gene. the b(0) allele seemed to be partially dominant over the a(0) allele, whereas b and c alleles appeared to map to quantitative trait loci (qtls), whic ...	2002	12582544
characterization of an abscisic acid responsive gene homologue from cucumis melo.	a cdna and genomic dna encoding an abscisic acid responsive gene (asr) homologue (asr1) was isolated from an inodorus melon, cucumis melo var. kuwata, cdna and genomic library. the asr1 gene showed the strongest fruit-specific expression and differential expression profiles during fruit development, which were expressed from a low copy gene. the promoter region of the asr1 gene contained several putative functional cis-elements, which may be involved in the response to plant hormones and environ ...	2002	12379794
cloning of disease-resistance homologues in end sequences of bac clones linked to fom-2, a gene conferring resistance to fusarium wilt in melon (cucumis melo l.).	disease resistance has not yet been characterized at the molecular level in cucurbits, a group of high-value, nutritious, horticultural plants. previously, we genetically mapped the fom-2 gene that confers resistance to fusarium wilt races 0 and i of melon. in this paper, two cosegregating codominant markers (am, aflp marker; fm, fusarium marker) were used to screen a melon bacterial artificial chromosome (bac) library. identified clones were fingerprinted and end sequenced. fingerprinting analy ...	2002	12033615
ultraviolet induced stress response in fresh cut cantaloupe.	changes in the composition of volatile compounds in cantaloupe melon (cucumis melo l. var. reticulatus) as a result of uv induced stress were determined by gas chromatography-mass spectrometry (gc-ms). several volatile ester compounds were present, of which twenty-seven were identified in fresh cut cantaloupe. fruit exposure to uv light decreased the concentrations of most of the aliphatic esters by over 60% of the amounts present in the corresponding fresh cut fruit. cyclic and acyclic terpenoi ...	2002	11985848
genetic control of fruit shape acts prior to anthesis in melon ( cucumis melo l.).	genetic control of fruit shape in cucumis melo was studied using qtl analysis in two recombinant inbred (ri) populations consisting of 163 and 63 individuals, respectively, obtained by crossing the same round-fruited parent with two different elongated-fruit lines. fruit shape is mainly explained by fruit length in these two populations. most qtls for fruit shape and ovary shape detected were found to co-segregate, thus demonstrating early control of fruit shape during ovary development. a high ...	2002	11862487
detection of ethylene receptor protein cm-ers1 during fruit development in melon (cucumis melo l.).	antibodies against melon ethylene receptor, cm- ers1 was prepared. cm-ers1 protein formed a disulphide-linked homodimer and it was present in microsomal membranes but not in soluble fractions. cm-ers1 protein was present at high levels in melon fruit during early developmental stages. this transition pattern was also observed in another melon cultivar.	2002	11847239
tgtcaca motif is a novel cis-regulatory enhancer element involved in fruit-specific expression of the cucumisin gene.	cucumisin, a subtilisin-like serine protease, is expressed at high levels in the fruit of melon (cucumis melo l.) and accumulates in the juice. we investigated roles of the promoter regions and dna-protein interactions in fruit-specific expression of the cucumisin gene. in transient expression analysis, a chimeric gene construct containing a 1.2-kb cucumisin promoter fused to a beta-glucuronidase (gus) reporter gene was expressed in fruit tissues at high levels, but the promoter activities in le ...	2002	11782472
volatile components in aqueous essence and fresh fruit of cucumis melo cv. athena (muskmelon) by gc-ms and gc-o.	a comparative study between the aromatic profile of muskmelon aqueous essence and the puree of fresh fruit was carried out using gas chromatography-mass spectrometry (gc-ms) and gas chromatography-olfactometry (gc-o). results obtained show a total of 53 components quantified in the essence and 38 in the fresh fruit. in addition, four new components are described for the first time as contributors to the aromatic profile of muskmelon including 2-methyl-3-buten-2-ol, 2,3-butanediol, methyl 3-pheny ...	2001	11743787
construction of a reference linkage map for melon.	a map of melon (cucumis melo l.) with 411 markers (234 rflps, 94 aflps, 47 rapds, 29 ssrs, five inter-ssrs, and two isozymes) and one morphological trait (carpel number) was constructed using the f2 progeny of a cross between the korean accession p1161375 and the spanish melon type 'pinyonet piel de sapo'. rflps were obtained using 212 probes from different genomic and cdna melon libraries, including 16 arabidopsis ests, 13 cucumis known genes, and three resistant gene homologues. most loci (391 ...	2001	11681608
resistance to diaphania hyalinata (lepidoptera: crambidae) in cucumis species.	diaphania hyalinata (l.), the melonworm, causes economic damage in cucurbit species in the caribbean region. to control larvae feeding on leaves, frequent pesticide applications are needed, but this favors outbreaks of other pests. we looked for plant resistance to d. hyalinata in cucumis melo l. and two wild cucumis, c. metuliferus meyer and c. pustulatus naudin. fifteen genotypes were observed using a 1-81 damage scale during 8 wk in two field trials in guadeloupe, french west indies. among th ...	2001	11425029
acetyl-coa: alcohol acetyltransferase activity and aroma formation in ripening melon fruits.	melon varieties (cucumis melo l.) differ in a range of physical and chemical attributes. sweetness and aroma are two of the most important factors in fruit quality and consumer preference. volatile acetates are major components of the headspace of ripening cv. arava fruits, a commercially important climacteric melon. in contrast, volatile aldehydes and alcohols are most abundant in cv. rochet fruits, a nonclimacteric melon. the formation of volatile acetates is catalyzed by alcohol acetyltransfe ...	2001	11262031
biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (cyp74c) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids.	a novel member of the plant cytochrome p450 cyp74 family of fatty acid hydroperoxide metabolizing enzymes has been cloned from melon fruit (cucumis melo). the cdna is comprised of 1,446 nucleotides encoding a protein of 481 amino acids. the homology at the amino acid level to other members of the cyp74 family is 35-50%, the closest relatives being allene oxide synthases. the cdna was expressed in escherichia coli, and the corresponding protein was purified by affinity column chromatography. the ...	2001	11368353
cloning and characterization of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme a reductase in melon (cucumis melo l. reticulatus).	we have isolated a cdna for cm-hmgr, encoding 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase in melon (cucumis melo l. reticulatus; genbank accession no. ab021862). cm-hmgr encodes a polypeptide of 588 amino acids that contains two transmembrane domains and a catalytic domain. database searches revealed that cm-hmgr shows homology to hmg1 (63.7%) and hmg2 (70.3%) of tomato, to hmg1 (77.2%) and hmg2 (69.4%) of arabidopsis thaliana, and to hmgr of tobacco (72.6%). functional expression ...	2001	11370859
examination of bacteriophage as a biocontrol method for salmonella on fresh-cut fruit: a model study.	the preparation and distribution of fresh-cut produce is a rapidly developing industry that provides the consumer with convenient and nutritious food. however, fresh-cut fruits and vegetables may represent an increased food safety concern because of the absence or damage of peel and rind, which normally help reduce colonization of uncut produce with pathogenic bacteria. in this study, we found that salmonella enteritidis populations can (i) survive on fresh-cut melons and apples stored at 5 degr ...	2001	11510645
salicylic acid and ethylene pathways are differentially activated in melon cotyledons by active or heat-denatured cellulase from trichoderma longibrachiatum.	infiltration of cellulase (ec 3.2.1.4) from trichoderma longibrachiatum into melon (cucumis melo) cotyledons induced several key defense mechanisms and hypersensitive reaction-like symptoms. an oxidative burst was observed 3 hours after treatment and was followed by activation of ethylene and salicylic acid (sa) signaling pathways leading to marked induction of peroxidase and chitinase activities. the treatment of cotyledons by heat-denatured cellulase also led to some induction of peroxidase an ...	2001	11553761
[microbiological stability study of minimally processed cantaloupe (cucumis melo l) by vacuum].	in order to design a minimal process for cantaloupe, the water activity of the fruit was reduced until 0.976 through vacuum osmotic dehydration and the optimum combination of microbial preservatives (100 ppm sodium sulfite, 600 ppm potassium sorbate, 0.5% l-ascorbic acid and 1% citric acid), that contributed to minimize (p < 0.05), the content of aerobic mesophilic bacteria, molds and yeast, was determined through a 2k multifactorial design. in the second experimental stage, the conditions previ ...	2001	11678049
[host plants of aphis gossypii (aphididae), vector of virus of cucumis melo melon (cucurbitaceae) in costa rica].	plant species associated with commercial melon crops and surrounding areas were examined to identity the natural host plants of aphis gossypii glover. the study was conducted in two farms located in different melon production areas and plant life zones of costa rica. plant species diversity, percent coverage and distribution over time were recorded during one year. differences between locations were observed. a total of 86 plant species (49 families) and 72 plant species (40 families) were ident ...	2001	11795159
identification of volatile compounds in cantaloupe at various developmental stages using solid phase microextraction.	using an automated rapid headspace solid phase microextraction (spme) method for volatile extraction in cantaloupes, 86 compounds already reported for muskmelons were recovered and an additional 53 compounds not previously reported were identified or tentatively identified. the spme method extracted a copious number of volatiles that can be analyzed to clearly differentiate between variety, growth stage, and stage of harvest ripeness. most of the newly reported compounds in cantaloupe were ester ...	2001	11312862
a hairy root culture of melon produces aroma compounds.	musk melon is the favorite fruit with a high market value in japan, and the fragrance is one of the major factors determining the fruit quality of melon. in this study, mutant melon hairy roots which had been induced by means of the t-dna insertion mutagenesis were found to produce volatile compounds with the fruity fragrance of mature melon. the volatile compounds were extracted and identified by glc-mass spectrometry. some essential oils such as (z)-3-hexenol, (e)-2-hexenal, 1-nonanol, and (z) ...	2000	10775407
cdna probes for detection of specific dsrnas from the fungal pathogen, monosporascus cannonballus.	monosporascus cannonballus is an ascomycete fungus that is the causative agent of monosporascus root rot/vine decline, a serious disease of muskmelon and watermelon. double-stranded rna (dsrna) was identified in approximately 60% of m. cannonballus isolates recovered from infected muskmelon plants in 1993. after repeated laboratory transfer on culture media, the majority of the isolates harboring dsrnas developed degenerate culture phenotypes and showed reduced virulence (hypovirulence) to muskm ...	2000	10680971
characterization of ripening-regulated cdnas and their expression in ethylene-suppressed charentais melon fruit.	charentais melons (cucumis melo cv reticulatus) are climacteric and undergo extremely rapid ripening. sixteen cdnas corresponding to mrnas whose abundance is ripening regulated were isolated to characterize the changes in gene expression that accompany this very rapid ripening process. sequence comparisons indicated that eight of these cdna clones encoded proteins that have been previously characterized, with one corresponding to acc (1-aminocyclopropane-1-carboxylic acid) oxidase, three to prot ...	2000	10712562
cucumisin-like protease from the latex of euphorbia supina.	a protease has been purified from the latex of euphorbia supina rafin by two steps of chromatography. the mr was estimated by sds-page to be 80 kda. its activity was inhibited strongly by diisopropyl fluorophosphate, but not by edta, pepstatin, or cysteine protease inhibitors, indicating that the enzyme is a serine protease. the specificity of the protease is broad, but the preferential cleavage sites were c-terminal sites of hydrophobic amino acid residues. the n-terminal sequence of the first ...	2000	10746875
cucumber mosaic virus infection affects sugar transport in melon plants.	viral infection often affects carbon assimilation and metabolism in host plants. to better understand the effect of cucumber mosaic virus (cmv) infection on sugar transport, carbohydrate levels and the amounts of the various sugars in the phloem sap were determined in infected melon (cucumis melo l.) plants. source leaves infected with cmv were characterized by high concentrations of reducing sugars and relatively low starch levels. the altered level of carbohydrates was accompanied by increased ...	2000	10859189
inhibitory serpins from wheat grain with reactive centers resembling glutamine-rich repeats of prolamin storage proteins. cloning and characterization of five major molecular forms.	genes encoding proteins of the serpin superfamily are widespread in the plant kingdom, but the properties of very few plant serpins have been studied, and physiological functions have not been elucidated. six distinct serpins have been identified in grains of hexaploid bread wheat (triticum aestivum l.) by partial purification and amino acid sequencing. the reactive centers of all but one of the serpins resemble the glutamine-rich repetitive sequences in prolamin storage proteins of wheat grain. ...	2000	10874043
identification of phloem involved in assimilate loading in leaves by the activity of the galactinol synthase promoter.	the definition of "minor" veins in leaves is arbitrary and of uncertain biological significance. generally, the term refers to the smallest vein classes in the leaf, believed to function in phloem loading. we found that a galactinol synthase promoter, cloned from melon (cucumis melo), directs expression of the gusa gene to the smallest veins of mature arabidopsis and cultivated tobacco (nicotiana tabacum) leaves. this expression pattern is consistent with the role of galactinol synthase in sugar ...	2000	10889241
comparative study on genomes of two japanese melon necrotic spot virus isolates.	nucleotide sequences of the genomes of two japanese melon necrotic spot virus (mnsv) isolates, nh and nk were determined. the open reading frames (orfs) in both genomes encode five proteins: p29 (the pre-readthrough domain of p89), p89 (the readthrough domain of p89 identified as the putative rna-dependent rna polymerase), p14 (the pre-readthrough domain of p7a), p7a (the putative movement protein), and p42 (coat protein, cp). nucleotide and amino acid sequence identities of the five proteins of ...	2000	11332272
the relationship of host range, physiology, and genotype to virulence on cantaloupe in pseudomonas syringae from cantaloupe blight epidemics in france.	abstract in 1993, a bacterial blight caused important losses of cantaloupe (cucumis melo var. cantalupensis) in southwestern france and has now been reported in all cantaloupe-growing regions of france. the causal agent of this blight is pseudomonas syringae, although on a worldwide basis this bacterium has not been a major pathogen of melon for over 50 years. to identify the pathovar of the cantaloupe pathogen, we employed biochemical tests, plasmid and chromosomal profiling, and host range stu ...	2000	18944544
simple sequence repeats in cucumis mapping and map merging.	thirty-four polymorphic simple-sequence repeats (ssrs) were evaluated for length polymorphism in melon (cucumis melo l.) and cucumber (cucumis sativus l.). ssr markers were located on three melon maps (18 on the map of 'vedrantais' and pi 161375, 23 on the map of 'piel de sapo' and pi 161375, and 16 on the map of pi 414723 and 'dulce'). in addition, 14 of the markers were located on the cucumber map of gy14 and pi 183967. ssrs proved to be randomly distributed throughout the melon and cucumber g ...	2000	11195350
polyamines and their cellular anti-senescence properties in honey dew muskmelon fruit.	activated oxygen free radicals cause peroxidative damage to all membranes and hasten senescence. polyamines (pas) are effective scavengers of these free radicals produced by lipoxygenase (lox) and phospholipase-d (pl-d). five days prior to abscission (harvest), 'honey brew' (cucumis melo l. (inodorus group)) fruit have a change in the ratio of endogenous spermidine (spd) to putrescine (put), from spd>put to spd<put, which coincides with the onset of fruit senescence. hypodermal-mesocarp tissues ...	2000	11164582
biochemical and microbial changes during the storage of minimally processed cantaloupe.	the effect of storage time on ph, titratable acidity, degrees brix, organic acids, sugars, amino acids, and color of minimally processed cantaloupe melon (cucumis melo l. var. reticulatus naud. cv. mission) was determined at 4 degrees c and 20 degrees c. changes in most of the biochemical parameters with storage time were relatively slow at the lower temperature. at 20 degrees c, a 17% loss in soluble solids and a 2-fold increase in acidity occurred after 2 days. organic acid content also increa ...	2000	11141266
randomized, double-blind, crossover challenge study in 53 subjects reporting adverse reactions to melon (cucumis melo).	few studies have evaluated ige-mediated hypersensitivity to melon with details of clinical reactions confirmed by double-blind, placebo-controlled, food challenges (dbpcfcs).	2000	11080722
the potential of lagenaria rootstock to confer resistance to the carmine spider mite, tetranychus cinnabarinus (acari: tetranychidae) in cucurbitaceae.	antibiosis and resistance of six cucurbita and two lagenaria accessions to the carmine spider mite, tetranychus cinnabarinus boisduval, were evaluated in the laboratory. significant differences among accessions were observed three days after the inoculation of detached leaf discs. the lagenaria accessions, slawi and sus, proved to be the most resistant to mites, with average populations of mite eggs, 87 and 95%, respectively less than that of the susceptible c. pepo accession, orangetti. the cuc ...	2000	10948370
cantaloupe melon peroxidase: characterization and effects of additives on activity.	peroxidase in cantaloupe melon (cucumis melo l. var. reticulatus naud.), a fruit commonly fresh cut processed, was characterized to determine reaction pathway, optimal conditions for activity and effect of some additives on enzymatic action. mn2+, cacl2, nano2 and kinetin had partial inhibitory effects on enzyme activity. activity was effectively inhibited by compounds capable of chelating peroxidase heme iron such as diethyldithiocarbamate and tiron, but unaffected by edta. free radical scaveng ...	2000	10907237
purification and kinetic characterization of an anionic peroxidase from melon (cucumis melo l.) cultivated under different salinity conditions.	the partial characterization of an anionic peroxidase in melon fruit is described. four melon peroxidase (mpx) isoenzymes were detected in crude extracts after isoelectric focusing. the major mpx isoenzyme (pi = 3.7) was partially purified by including hydrophobic and anion-exchange chromatography in the purification scheme. the sample obtained was used to characterize mpx. this peroxidase did not show activity on ascorbic acid but oxidized guaiacol at a high rate, showing an optimum ph of 5.5 w ...	2000	10820055
stimulation of benzyladenine-induced in vitro shoot organogenesis and endogenous proline in melon (cucumis melo l.) by fish protein hydrolysates in combination with proline analogues.	a previous study demonstrated that proline is beneficial for improving melon in vitro shoot organogenesis. a natural source of proline and proline precursors can be obtained from fish protein hydrolysates (fph), a byproduct of the fishery industry. proline analogues azetidine-2-carboxylate and hydroxyproline in combination with standardized fph were used to stimulate proline synthesis and benzyladenine-induced shoot organogenesis by exploiting the proposed proline-linked pentose phosphate pathwa ...	1999	10564053
stage- and tissue-specific expression of ethylene receptor homolog genes during fruit development in muskmelon.	we isolated two muskmelon (cucumis melo) cdna homologs of the arabidopsis ethylene receptor genes etr1 and ers1 and designated them cm-etr1 (c. melo etr1; accession no. af054806) and cm-ers1 (c. melo ers1; accession no. af037368), respectively. northern analysis revealed that the level of cm-ers1 mrna in the pericarp increased in parallel with the increase in fruit size and then markedly decreased at the end of enlargement. in fully enlarged fruit the level of cm-ers1 mrna was low in all tissues ...	1999	10318709
a novel alkaline alpha-galactosidase from melon fruit with a substrate preference for raffinose	the cucurbits translocate the galactosyl-sucrose oligosaccharides raffinose and stachyose, therefore, alpha-galactosidase (alpha-d-galactoside galactohydrolase, ec 3.2.1.22) is expected to function as the initial enzyme of photoassimilate catabolism. however, the previously described alkaline alpha-galactosidase is specific for the tetrasaccharide stachyose, leaving raffinose catabolism in these tissues as an enigma. in this paper we report the partial purification and characterization of three ...	1999	10069835
nymphal survival and movement of crawlers of bemisia argentifolii (homoptera: aleyrodidae) on leaf surfaces of selected vegetables.	bemisia argentifolii bellows & perring generally feeds and develops on the under leaf surface of most host plants, but in excess of 50% of the immature population may be found on the upper surface of some host species. this study determined the influence of leaf surface on survival of immature b. argentifolii and the likelihood of movement by the crawler (1st instar) from the upper (adaxial) leaf surface on selected vegetable hosts. laboratory, tests were conducted on 5 vegetables: cantaloupe, c ...	1999	11543187
potassium permanganate staining for differentiation the surface morphology of opisthorchis viverrini, haplorchis taichui and phaneropsolus bonnei eggs.	potassium permanganate staining method was developed for differentiation opisthorchis viverrini, haplorchis taichui and phaneropsolus bonnei eggs. the surfaces of o. viverrini, h. taichui and p. bonnei eggs stained permanently and temporarily were similar in appearance even the staining procedures were varied both in concentration and time. determined under light microscope set at 400x, all of these eggs were oval-shaped, operculated at one pole and indistinct small knob at posterior end. o. viv ...	1999	10774711
evaluation of botulinal toxin production in packaged fresh-cut cantaloupe and honeydew melons.	the ability of clostridium botulinum to produce toxin on cubed, packaged melons was investigated relative to microbial spoilage at various incubation temperatures and in different packaging systems. freshly cut cubes (approximately 2.5 cm3) of cantaloupe and honeydew melons were surface inoculated with a 10 strain mixture of proteolytic and nonproteolytic spores of c. botulinum (10 to 15 cubes per package; approximately 100 total spores per package). to initially evaluate toxin production and sp ...	1999	10456752
a new subtilisin-like proteinase from roots of the dandelion taraxacum officinale webb s. l.	a serine proteinase from roots of taraxacum officinale webb s. l. was isolated by affinity chromatography and gel-filtration on superose 6r using fplc. the enzyme is a 67-kd glycoprotein containing 54% carbohydrate which we have named taraxalisin. the substrate specificity of taraxalisin toward synthetic peptides and oxidized insulin b-chain is comparable with that of cucumisin from cucumis melo and the subtilisin-like serine proteinase macluralisin from maclura pomifera. the proteinase is inact ...	1999	10521720
development and utility of cleaved amplified polymorphic sequences (caps) and restriction fragment length polymorphisms (rflps) linked to the fom-2 fusarium wilt resistance gene in melon (cucumis melo l.).	fusarium wilt, caused by fusarium oxysporum schlecht f. sp. melonis snyder & hans, is a worldwide soil-borne disease of melon (cucumis melo l.). resistance to races 0 and 1 of fusarium wilt is conditioned by the dominant gene fom-2. to facilitate marker-assisted backcrossing with selection for fusarium wilt resistance, we developed cleaved amplified polymorphic sequences (caps) and restriction fragment length polymorphisms (rflp) markers by converting rapd markers e07 (a 1.25-kb band) and g17 (a ...	1999	22665178
role of ethylene and abscisic acid in physicochemical modifications during melon ripening.	hormonal metabolism associated with fruit ripening in two cantaloupe muskmelon cultivars, talma and manta, has been studied. the ethylene crisis began on day 33 after fruit set, reaching the maximum values of internal ethylene concentration and ethylene production rate on day 35 after fruit set. this was the optimum moment for consumption as shown by the higher content in soluble solids, ripening index, sensory analysis, and color parameter values. the b parameter and the b/a quotient values in ...	1999	10606610
cloning, expression, and role in pathogenicity of pg1 encoding the major extracellular endopolygalacturonase of the vascular wilt pathogen fusarium oxysporum.	pg1 encoding the major in vitro extracellular endopolygalacturonase of the tomato vascular wilt pathogen fusarium oxysporum f. sp. lycopersici was cloned and sequenced. the deduced mature protein had a calculated molecular mass of 35.5 kda and a pi of 6.2, and showed significant similarity with other fungal endopgs. pg1 mrna was induced in vitro by citrus pectin, tomato vascular tissue, 0.1% d-galacturonic acid, and polygalacturonic acid, and repressed by 1% d-galacturonic acid and 1% glucose. r ...	1998	9450333
endopolygalacturonase pg1 in different formae speciales of fusarium oxysporum	pg1, the major endopolygalacturonase of the vascular wilt pathogen fusarium oxysporum, was secreted during growth on pectin by 10 of 12 isolates belonging to seven formae speciales, as determined with isoelectric focusing zymograms and sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. a southern analysis of genomic dna and pcr performed with gene-specific primers revealed that the pg1 locus was highly conserved structurally in most isolates. two pg1-deficient isolates were identifi ...	1998	9572983
polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly.	ripening-associated pectin disassembly in melon is characterized by a decrease in molecular mass and an increase in the solubilization of polyuronide, modifications that in other fruit have been attributed to the activity of polygalacturonase (pg). although it has been reported that pg activity is absent during melon fruit ripening, a mechanism for pg-independent pectin disassembly has not been positively identified. here we provide evidence that pectin disassembly in melon (cucumis melo) may be ...	1998	9625689
pathogenic and genetic variation in the japanese strains of fusarium oxysporum f. sp. melonis.	abstract pathogenic variation among 41 japanese strains of fusarium oxysporum f. sp. melonis was analyzed by pathogenicity tests with muskmelon, oriental melon, and oriental pickling melon cultivars. based on pathogenicity to muskmelon cvs. amus and ohi and oriental melon cv. ogon 9, 41 strains were divided into 3 groups that corresponded completely to risser's races 0, 2, and 1,2y. to further characterize pathogenic variation within the forma specialis and races, strains were assayed for pathog ...	1998	18944886
cucumisin like protease from the sarcocarp of benincasa hispida var. ryukyu.	a protease has been purified from the sarcocarp of benincasa hispida (thunb.) cogn. var. ryukyu by two steps of chromatography. its m(r) was estimated by sds-page to be about 67,000. the enzyme was strongly inhibited by diisopropyl fluorophosphate, but not by edta and cysteine protease inhibitors. the substrate having alanine at the position of p1 was the best among the ala-ala-pro-x-pnas (x = ala, lys, phe, glu, and diaminopropionic acid (dap)). the n-terminal sequence of the first 33 residues ...	1998	9887522
callose deposition is responsible for apoplastic semipermeability of the endosperm envelope of muskmelon seeds	semipermeable cell walls or apoplastic "membranes" have been hypothesized to be present in various plant tissues. although often associated with suberized or lignified walls, the wall component that confers osmotic semipermeability is not known. in muskmelon (cucumis melo l.) seeds, a thin, membranous endosperm completely encloses the embryo, creating a semipermeable apoplastic envelope. when dead muskmelon seeds are allowed to imbibe, solutes leaking from the embryo are retained within the enve ...	1998	9733528
temporal sequence of cell wall disassembly in rapidly ripening melon fruit	the charentais variety of melon (cucumis melo cv reticulatus f1 alpha) was observed to undergo very rapid ripening, with the transition from the preripe to overripe stage occurring within 24 to 48 h. during this time, the flesh first softened and then exhibited substantial disintegration, suggesting that charentais may represent a useful model system to examine the temporal sequence of changes in cell wall composition that typically take place in softening fruit. the total amount of pectin in th ...	1998	9625688
characterization of cdnas encoding small and large subunits of adp-glucose pyrophosphorylases from watermelon (citrullus vulgaris s.).	three cdna clones encoding adp-glucose pyrophosphorylases were isolated from a full red fruit cdna library of watermelon (citrullus vulgaris s.). sequence analyses indicated that one clone, wms1, corresponds to the small subunit, and two clones, wml1 and wml2 (a partial gene), are the large subunits of agpase. the presumed agpase proteins encoded by wms1, wml1, and wml2 have 526, 526, and 481 amino acids, respectively. the protein sequences have the conserved amino acids important for the substr ...	1998	9571786
substrate specificity of honeydew melon protease d, a plant serine endopeptidase.	the substrate specificity of honeydew melon (cucumis melo var. inodorus naud) protease d was studied by the use of synthetic substrates and oligopeptides derived from a protein hydrolyzate. the hydrolysis rates of succinyl-(l-ala)1-3-p-nitroanilide (suc-(ala)1-3-pna) the hydrolysis rate progressively rose in proportion to the increased chain length. benzyloxycarbonyl-l-tyrosine p-nitrophenyl ester (z-tyr-onp) and benzoyl-l-tyrosine ethyl ester (bz-tyr-oet) were cleaved by honeydew melon protease ...	1997	9301107
ethanol-induced anaphylaxis following ingestion of overripe rock melon, cucumis melo.	anaphylactic reactions to ethanol are rare with only seven cases reported in the literature. all previous cases have occurred following ingestion of alcoholic beverages or food to which an alcoholic beverage has been added. to date there are no cases in the literature of ethanol-induced anaphylaxis caused by the accumulation of endogenous ethanol in overripe fruit.	1997	9087153
purification and some properties of a protease from the sarcocarp of musk melon fruit.	a protease has been purified from sarcocarp of musk melon, cucumis melo ssp. melo var. reticulatus naud. earl's favourite. the protease was mostly present in the placenta part of the fruit and next in the inside mesocarp. the molecular mass of the enzyme was estimated to be about 62kda on sds-page. the enzyme had a carbohydrate moiety. the optimum ph of the enzyme was 11 at 35°c using casein as a substrate. the enzyme was stable between ph 6 and 11. the enzyme was strongly inhibited by diisoprop ...	1997	27396886
characterization of two cdna clones for mrnas expressed during ripening of melon (cucumis melo l.) fruits.	in vitro translation of mrnas and polyacrylamide gel electrophoresis of proteins from melons revealed that several mrnas increased in amount during ripening, indicating the existence of other ripening genes in addition to those cloned previously. to identify ripening-related genes we have screened a ripe melon cdna library and isolated two novel cdna clones (mel2 and mel7) encoding unidentified proteins. southern analysis revealed that mel2 and mel7 are encoded by low-copy-number genes. the mel2 ...	1997	9037149
transfer of the yeast salt tolerance gene hal1 to cucumis melo l. cultivars and in vitro evaluation of salt tolerance.	an agrobacterium-mediated gene transfer method for production of transgenic melon plants has been optimized. the hal1 gene, an halotolerance gene isolated from yeast, was inserted in a chimaeric construct and joined to two marker genes: a selectable-neomycin phosphotransferase-ii (nptii)-, and a reporter-beta-glucuronidase (gus)-. the entire construct was introduced into commercial cultivars of melon. transformants were selected for their ability to grow on media containing kanamycin. transforma ...	1997	9032977
characterization of epitopes on zucchini yellow mosaic potyvirus coat protein permits studies on the interactions between strains.	monoclonal antibodies (mabs) raised against the coat protein of zucchini yellow mosaic potyvirus (zymv) were characterized by epitope mapping using synthetic oligopeptides. two mutant viruses with a mutation in the amino acid sequence important for epitope recognition in vitro were obtained by site-directed mutagenesis of a full-length cdna of zymv. two mabs, cc11 and dd2, could distinguish specifically between these mutants in mixed infections, or after sequential inoculations of muskmelons. se ...	1997	9267009
a genetic map of melon (cucumis melo l.) with rflp, rapd, isozyme, disease resistance and morphological markers.	one hundred and ten markers were analysed for linkage in 218 f2 plants derived from two divergent cultivars ('védrantais' and 'songwhan charmi') of cucumis melo (l.). thirty-four rflps, 64 rapds, one isozyme, four disease resistance markers and one morphological marker were used to construct a genetic map spanning 14 linkage groups covering 1390 cm of the melon genome. rapd and rflp markers detected similar polymorphism levels. rflps were largely due to base substitutions rather than insertion/d ...	1996	24162199
length polymorphism and homologies of microsatellites in several cucurbitaceae species.	the objectives of this research were to assess (1) the degree of simple sequence repeats (ssr) dna length polymorphism in melon (cucumis melo l.) and other species within the cucurbitaceae family and (2) the possibility of utilizing ssrs flanking primers from single species to other genera or species of cucurbitaceae. five melon (ct/ga) n ssrs were isolated from a genomic library. two cucumber (cucumis sativus l.) ssrs were detected through a search of dna sequence databases, one contained a (ct ...	1996	24162541