Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| [genome-wide analysis of hsp70 superfamily in gossypium raimondii and the expression of orthologs in gossypium hirsutum]. | heat shock 70 (hsp70) proteins are highly conserved molecular chaperones widely existed in the plant kingdom which are involved in cellular protein folding process. in this study, comprehensive evolutionary analyses of the gossypium raimondii hsp70 gene family members are conducted and 30 hsp70 genes are identified. the gene structure, chromosome distribution, gene duplication and phylogenic evolution of this family are further analyzed. the results reveal that hsp70 family genes can be clustere ... | 2014 | 25252310 |
| the cotton wrky transcription factor ghwrky17 functions in drought and salt stress in transgenic nicotiana benthamiana through aba signaling and the modulation of reactive oxygen species production. | drought and high salinity are two major environmental factors that significantly limit the productivity of agricultural crops worldwide. wrky transcription factors play essential roles in the adaptation of plants to abiotic stresses. however, wrky genes involved in drought and salt tolerance in cotton (gossypium hirsutum) are largely unknown. here, a group iid wrky gene, ghwrky17, was isolated and characterized. ghwrky17 was found to be induced after exposure to drought, salt, h2o2 and aba. the ... | 2014 | 25261532 |
| genetic mapping of fiber color genes on two brown cotton cultivars in xinjiang. | in the present study, genetic linkage analysis was carried out to map the fiber color loci lc 1 and lc 2 on two brown cotton cultivars with ssr and est-ssr markers in the reference map by f2 segregation populations. the lc 1 locus carried by xincaimian6 (gossypium hirsutum l.) was flanked by the marker nau2862 and nau1043 on the long arm of chromosome 07, with genetic distance 7.8 cm and 3.8 cm, respectively. the lc 2 carried by xincaimian 5 (gossypium hirsutum l.) was flanked by the marker nau5 ... | 2014 | 25207203 |
| proteomic analysis of anthers from wild-type and photosensitive genetic male sterile mutant cotton (gossypium hirsutum l.). | male sterility is a common phenomenon in flowering plant species, and it has been successfully developed in several crops by taking advantage of heterosis. using space mutation breeding of upland cotton, a novel photosensitive genetic male sterile (pgms) mutant was isolated. to take advantage of the pgms lines in cotton hybrid breeding, it is of great importance to study the molecular mechanisms of its male sterility. | 2014 | 25547499 |
| characterization of the global transcriptome for cotton (gossypium hirsutum l.) anther and development of ssr marker. | cotton is an important fiber plant, and it's attractive to elucidate the molecular mechanism of anther development due to the close relationship between the anther fertility and boll-setting, and also fiber yield. in the present paper, 47.2 million paired-end reads with average length of 82.87 bp from the anthers of tm-1 (gossypium hirsutum l.), a genetic standard line, were generated through transcriptome sequencing, and 210,965 unigenes of more than 100 bp were obtained. blast, kegg, cog, and ... | 2014 | 25178523 |
| direct visualization of horizontal gene transfer in cotton plants. | plant mitochondrial and chloroplast genes that underwent horizontal transfer have been identified by parasite and grafting systems, respectively. here, we directly observed 3 horizontal gene transfer (hgt) events in the 45 second axillary shoots of grafted cotton plants (gossypium barbadense and gossypium hirsutum) after extirpating the first axillary bud. the second axillary shoots showed phenotypic variations in cotton flowers and seeds that were evidence of spontaneous development from cells ... | 2014 | 25160847 |
| carcass merit and meat quality in suffolk lambs, katahdin lambs, and meat-goat kids finished on a grass-legume pasture with and without supplementation. | the experiment evaluated traditional u.s. sheep (suffolk), hair sheep (katahdin), and meat goat (boer crossbred; goat) carcass and meat quality parameters when finished on pasture with and without supplemental whole cottonseed (gossypium hirsutum l.). supplemented animals had greater ribeye area (p<0.01), body wall thickness (p<0.001), and lean quality score (p<0.05) than unsupplemented animals. whole cottonseed increased fatty acids (fa) 18:1 trans-10, 18:1 trans-12, 18:2, and omega6:omega3 rat ... | 2014 | 24971809 |
| identification of centromeric regions on the linkage map of cotton using centromere-related repeats. | centromere usually contains high-copy-number retrotransposons and satellite repeats, which are difficult to map, clone and sequence. currently, very little is known about the centromere in cotton. here, we sequenced a bacterial artificial chromosome (bac) mapping to the centromeric region and predicted four long-terminal-repeat (ltr) retrotransposons. they were located in the heterochromatic centromeric regions of all 52 pachytene chromosomes in gossypium hirsutum. fiber-fish mapping revealed th ... | 2014 | 25238895 |
| performance of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae) under variable conditions of temperature and mating. | mealybugs have strong associations with their host plants due to their limitations for dispersal. thus, environmental conditions and host quality may impact the biological traits of mealybugs. to the best of our knowledge, we are the first to report on the biology of a brazilian population of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae), which has recently been reported to infest cotton in brazil. we evaluated the development and reproductive performance of f. virg ... | 2014 | 27193398 |
| seed-specific expression of a lysine-rich protein gene, ghlrp, from cotton significantly increases the lysine content in maize seeds. | maize seed storage proteins are a major source of human and livestock consumption. however, these proteins have poor nutritional value, because they are deficient in lysine and tryptophan. much research has been done to elevate the lysine content by reducing zein content or regulating the activities of key enzymes in lysine metabolism. using the naturally lysine-rich protein genes, sb401 and sbglr, from potato, we previously increased the lysine and protein contents of maize seeds. here, we exam ... | 2014 | 24681583 |
| mir828 and mir858 regulate homoeologous myb2 gene functions in arabidopsis trichome and cotton fibre development. | although polyploidy is common in plants and some animals, mechanisms for functional divergence between homoeologous genes are poorly understood. myb2 gene promotes cotton fibre development and is functionally homologous to arabidopsis glabrous1 (gl1) in trichome formation. the most widely cultivated cotton is an allotetraploid (gossypium hirsutum, aadd) that contains ghmyb2a and ghmyb2d homoeologs. here we show that ghmyb2d mrna accumulates more than ghmyb2a during fibre initiation and is target ... | 2014 | 24430011 |
| a peptide hormone gene, ghpsk promotes fibre elongation and contributes to longer and finer cotton fibre. | cotton fibres, the single-celled trichomes derived from the ovule epidermis, provide the most important natural material for the global textile industry. a number of studies have demonstrated that regulating endogenous hormone levels through transgenic approaches can improve cotton fibre qualities. phytosulfokine-α (psk-α) is a novel peptide hormone in plants that is involved in regulating cell proliferation and elongation. however, its potential applications in crop genetic improvement have not ... | 2014 | 24666593 |
| genetic dissection of upland cotton (gossypium hirsutum) cultivars developed in hubei province by mapped ssrs. | the genetic diversity of 51 upland cotton cultivars with different parental origins and breeding periods that were developed in hubei province was studied on the basis of 237 mapped simple sequence repeat markers covering the cotton genome. a total of 108 polymorphic primer pairs amplified 196 loci; the polymorphism information content range was 0.04 to 0.83, with an average of 0.46. a model-based clustering analysis (structure) of the genomic data identified 3 clear subpopulations, and the resu ... | 2014 | 24615042 |
| cellulose synthases: new insights from crystallography and modeling. | detailed information about the structure and biochemical mechanisms of cellulose synthase (cels) proteins remained elusive until a complex containing the catalytic subunit (bcsa) of cels from rhodobacter sphaeroides was crystalized. additionally, a 3d structure of most of the cytosolic domain of a plant cels (ghcesa1 from cotton, gossypium hirsutum) was produced by computational modeling. this predicted structure contributes to our understanding of how plant cels proteins may be similar and diff ... | 2014 | 24139443 |
| reflectance and cyclic electron flow as an indicator of drought stress in cotton (gossypium hirsutum). | the response and the functioning of the photosynthetic machinery of cotton, gossypium hirsutum during water stress was studied by leaf optical properties, linear (etrii) and cyclic electron flow (cef) and chlorophyll a fluorescence. we observed that in g. hirsutum, during water limitation, chlorophyll b showed the best correlation with reflectance at 731 nm and is a better indicator of drought. fv /fm was observed to be very insensitive to mild water stress. however, during severe water stress t ... | 2014 | 24246098 |
| identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from gossypium hirsutum. | plants have developed a variety of adaptive mechanisms to cope with stresses. a novel salt-induced gene was isolated during the screening of a nacl-induced cdna library of cotton seedlings. the gene was registered as accession number ay972810 in genbank. phylogenetic analysis suggested that the protein encoded by the gene belongs to the parvulin family of peptidyl-prolyl cis/trans isomerases (ppiases, ec 5.2.1.8). northern blot analysis indicated that the mrna accumulation of ghppi was induced b ... | 2014 | 24468661 |
| comparative evolutionary and developmental dynamics of the cotton (gossypium hirsutum) fiber transcriptome. | the single-celled cotton (gossypium hirsutum) fiber provides an excellent model to investigate how human selection affects phenotypic evolution. to gain insight into the evolutionary genomics of cotton domestication, we conducted comparative transcriptome profiling of developing cotton fibers using rna-seq. analysis of single-celled fiber transcriptomes from four wild and five domesticated accessions from two developmental time points revealed that at least one-third and likely one-half of the g ... | 2014 | 24391525 |
| the calcium sensor ghcam7 promotes cotton fiber elongation by modulating reactive oxygen species (ros) production. | fiber elongation is the key determinant of fiber quality and output in cotton (gossypium hirsutum). although expression profiling and functional genomics provide some data, the mechanism of fiber development is still not well understood. here, a gene encoding a calcium sensor, ghcam7, was isolated based on its high expression level relative to other ghcams in fiber cells at the fast elongation stage. the level of expression of ghcam7 in the wild-type and the fuzzless/lintless mutant correspond t ... | 2014 | 24443839 |
| phytochrome rnai enhances major fibre quality and agronomic traits of the cotton gossypium hirsutum l. | simultaneous improvement of fibre quality, early-flowering, early-maturity and productivity in upland cotton (g. hirsutum) is a challenging task for conventional breeding. the influence of red/far-red light ratio on the fibre length prompted us to examine the phenotypic effects of rna interference (rnai) of the cotton phya1 gene. here we show a suppression of up to ~70% for the phya1 transcript, and compensatory overexpression of up to ~20-fold in the remaining phytochromes in somatically regene ... | 2014 | 24430163 |
| ghmpk17, a cotton mitogen-activated protein kinase, is involved in plant response to high salinity and osmotic stresses and aba signaling. | mitogen-activated protein kinase (mapk) cascades play pivotal roles in mediating biotic and abiotic stress responses. cotton (gossypium hirsutum) is the most important textile crop in the world, and often encounters abiotic stress during its growth seasons. in this study, a gene encoding a mitogen-activated protein kinase (mapk) was isolated from cotton, and designated as ghmpk17. the open reading frame (orf) of ghmpk17 gene is 1494 bp in length and encodes a protein with 497 amino acids. quanti ... | 2014 | 24743296 |
| within-field spatial distribution of stink bug (hemiptera: pentatomidae)-induced boll injury in commercial cotton fields of the southeastern united states. | spatial distribution of boll injury caused by stink bugs to developing cotton (gossypium hirsutum l.) bolls was studied in five commercial fields (≍22 ha each) in 2011 and 2012 to understand variability in boll injury dynamics within fields. cotton bolls and stink bugs were sampled weekly from a georeferenced grid of sampling points (one sample per 0.40 ha) in each field, but no samples were taken within 30 m of field edges. the inverse distance weighted interpolation, variogram analysis, and mo ... | 2014 | 24735500 |
| quantitative phosphoproteomics analysis of nitric oxide-responsive phosphoproteins in cotton leaf. | knowledge of phosphorylation events and their regulation is crucial to understanding the functional biology of plant proteins, but very little is currently known about nitric oxide-responsive phosphorylation in plants. here, we report the first large-scale, quantitative phosphoproteome analysis of cotton (gossypium hirsutum) treated with sodium nitroprusside (nitric oxide donor) by utilizing the isobaric tag for relative and absolute quantitation (itraq) method. a total of 1315 unique phosphopep ... | 2014 | 24714030 |
| modelling the structural response of cotton plants to mepiquat chloride and population density. | cotton (gossypium hirsutum) has indeterminate growth. the growth regulator mepiquat chloride (mc) is used worldwide to restrict vegetative growth and promote boll formation and yield. the effects of mc are modulated by complex interactions with growing conditions (nutrients, weather) and plant population density, and as a result the effects on plant form are not fully understood and are difficult to predict. the use of mc is thus hard to optimize. | 2014 | 24489020 |
| related to aba-insensitive3(abi3)/viviparous1 and atabi5 transcription factor coexpression in cotton enhances drought stress adaptation. | drought tolerance is an important trait being pursued by the agbiotech industry. abscisic acid (aba) is a stress hormone that mediates a multitude of processes in growth and development, water use efficiency (wue) and gene expression during seed development and in response to environmental stresses. arabidopsis b3-domain transcription factor related to aba-insensitive3 (abi3)/viviparous1 (namely atrav2) and basic leucine zipper (bzips) atabi5 or atabf3 transactivated aba-inducible promoter:gus r ... | 2014 | 24483851 |
| aberrant phenotype and transcriptome expression during fiber cell wall thickening caused by the mutation of the im gene in immature fiber (im) mutant in gossypium hirsutum l. | the immature fiber (im) mutant of gossypium hirsutum l. is a special cotton fiber mutant with non-fluffy fibers. it has low dry weight and fineness of fibers due to developmental defects in fiber secondary cell wall (scw). | 2014 | 24483163 |
| both methylerythritol phosphate and mevalonate pathways contribute to biosynthesis of each of the major isoprenoid classes in young cotton seedlings. | in higher plants, both the methylerythritol phosphate (mep) and mevalonate (mva) pathways contribute to the biosynthesis of isoprenoids. however, despite a significant amount of research on the activity of these pathways under different conditions, the relative contribution of each to the biosynthesis of diverse isoprenoids remains unclear. in this work, we examined the formation of several classes of isoprenoids in cotton (gossypium hirsutum l.). after feeding [5,5-(2)h2]-1-deoxy-d-xylulose ([5 ... | 2014 | 24359633 |
| waterlogging during flowering and boll forming stages affects sucrose metabolism in the leaves subtending the cotton boll and its relationship with boll weight. | the work explored sucrose metabolism in the leaves subtending the cotton boll (sbl) and its role in boll weight after waterlogging in cotton. results showed that net photosynthesis rate (pn), relative water content, contents of chlorophyll a and chlorophyll b, initial ribulose-1,5-bisphosphate carboxylase-oxygenase (rubisco) activity and cytosolic fructose-1, 6-bisphosphatase (cy-fbpase) activity decreased with waterlogging in the sbl on fruiting branches 2-3 (fb2-3) and fb6-7. activities of suc ... | 2014 | 24767118 |
| comparative transcriptome analysis of short fiber mutants ligon-lintless 1 and 2 reveals common mechanisms pertinent to fiber elongation in cotton (gossypium hirsutum l.). | understanding the molecular processes affecting cotton (gossypium hirsutum) fiber development is important for developing tools aimed at improving fiber quality. short fiber cotton mutants ligon-lintless 1 (li1) and ligon-lintless 2 (li2) are naturally occurring, monogenic mutations residing on different chromosomes. both mutations cause early cessation in fiber elongation. these two mutants serve as excellent model systems to elucidate molecular mechanisms relevant to fiber length development. ... | 2014 | 24748059 |
| gene expression profile analysis of ligon lintless-1 (li1) mutant reveals important genes and pathways in cotton leaf and fiber development. | ligon lintless-1 (li1) is a monogenic dominant mutant of gossypium hirsutum (upland cotton) with a phenotype of impaired vegetative growth and short lint fibers. despite years of research involving genetic mapping and gene expression profile analysis of li1 mutant ovule tissues, the gene remains uncloned and the underlying pathway of cotton fiber elongation is still unclear. in this study, we report the whole genome-level deep-sequencing analysis of leaf tissues of the li1 mutant. differentially ... | 2014 | 24279997 |
| the role of somatic embryogenesis receptor-like kinase 1 in controlling pollen production of the gossypium anther. | in flowering plants, male gametophytes are generated in anthers from microsporocytes. however, more evidence is needed to reveal the genetic mechanisms which regulate the differentiation and interaction of these highly specialized cells in anthers. here we report the characterization of a series of male-sterile cotton (gossypium hirsutum) mutants, including mutants with normal fertility, semi-sterility and complete sterility. these mutants are forms of transgenic cotton containing rnai vectors w ... | 2014 | 24276918 |
| the li2 mutation results in reduced subgenome expression bias in elongating fibers of allotetraploid cotton (gossypium hirsutum l.). | next generation sequencing (rna-seq) technology was used to evaluate the effects of the ligon lintless-2 (li2) short fiber mutation on transcriptomes of both subgenomes of allotetraploid cotton (gossypium hirsutum l.) as compared to its near-isogenic wild type. sequencing was performed on 4 libraries from developing fibers of li2 mutant and wild type near-isogenic lines at the peak of elongation followed by mapping and polycat categorization of rna-seq data to the reference d5 genome (g. raimond ... | 2014 | 24598808 |
| changes during leaf expansion of φpsii temperature optima in gossypium hirsutum are associated with the degree of fatty acid lipid saturation. | in this project, we hypothesize that cotton (gossypium hirsutum) leaf temperature and the responses of leaf photosynthesis to temperature will change as the leaves expand and that differences between young and mature leaves will be associated with the proportion of saturated fatty acids in thylakoid and other membrane lipids. to that end, we studied main stem leaves obtained from plants growing in a temperature controlled greenhouse and at different times in the field season. we found that young ... | 2014 | 24594393 |
| the effects of fruiting positions on cellulose synthesis and sucrose metabolism during cotton (gossypium hirsutum l.) fiber development. | cotton (gossypium hirsutum l.) boll positions on a fruiting branch vary in their contribution to yield and fiber quality. fiber properties are dependent on deposition of cellulose in the fiber cell wall, but information about the enzymatic differences in sucrose metabolism between these fruiting positions is lacking. therefore, two cotton cultivars with different sensitivities to low temperature were tested in 2010 and 2011 to quantify the effect of fruit positions (fps) on fiber quality in rela ... | 2014 | 24586807 |
| molecular characterization of cotton c-repeat/dehydration-responsive element binding factor genes that are involved in response to cold stress. | low temperature, drought and salinity are major abiotic stresses that influence survival, productivity and geographical distribution of many important crops across the globe. the c-repeat/dehydration-responsive element binding transcription factors (cbf/dreb) are important proteins involved in response to abiotic stresses in plants. in this study, twenty-one cbf genes were identified in cotton (gossypium hirsutum) by bioinformatic approach. the twenty-one cbf genes (named as ghcbf1--ghcbf21) wer ... | 2014 | 24566693 |
| a mads-box gene is specifically expressed in fibers of cotton (gossypium hirsutum) and influences plant growth of transgenic arabidopsis in a ga-dependent manner. | in this study, a cdna, ghmads14, encoding a typical mads-box protein with 223 amino acids was isolated from a cotton cdna library. fluorescent microscopy indicated that the ghmads14 protein was localized in the cell nucleus. ghmads14 was specifically expressed in the elongating fibers, and its expression was gradually enhanced at early stages of fiber elongation and reached its peak in 9-10 dpa fibers. overexpression of ghmads14 in arabidopsis hindered plant growth. measurement and statistical a ... | 2014 | 24374505 |
| comparative transcriptome analysis between somatic embryos (ses) and zygotic embryos in cotton: evidence for stress response functions in se development. | as a product of asexual reproduction in plants, the somatic embryo (se) differentiates into a new plantlet via a zygotic embryogenesis-like process. here, we present the phenotypic and cellular differences between ses and zygotic embryos (zes) revealed by histological section scanning using three parallel development stages of the two types of embryos of cotton (gossypium hirsutum cv. yz1), including globular, torpedo and cotyledonary-stages. to identify the molecular characteristics of se devel ... | 2014 | 24112122 |
| changes in the cell wall and cellulose content of developing cotton fibers investigated by ftir spectroscopy. | fourier transform infrared (ftir) spectra of cotton fibers harvested at different stages of development were acquired using universal attenuated total reflectance ftir (uatr-ftir). the main goal of the study was to monitor cell wall changes occurring during different phases of cotton fiber development. two cultivars of gossypium hirsutum l. were planted in a greenhouse (texas marker-1 and tx55). on the day of flowering, individual flowers were tagged and bolls were harvested. from fibers harvest ... | 2014 | 24188832 |
| genetic diversity and population structure in the us upland cotton (gossypium hirsutum l.). | genetic diversity and population structure in the us upland cotton was established and core sets of allelic richness were identified for developing association mapping populations in cotton. elite plant breeding programs could likely benefit from the unexploited standing genetic variation of obsolete cultivars without the yield drag typically associated with wild accessions. a set of 381 accessions comprising 378 upland (gossypium hirsutum l.) and 3 g. barbadense l. accessions of the united stat ... | 2014 | 24170350 |
| mapping and genomic targeting of the major leaf shape gene (l) in upland cotton (gossypium hirsutum l.). | a major leaf shape locus (l) was mapped with molecular markers and genomically targeted to a small region in the d-genome of cotton. by using expression analysis and candidate gene mapping, two lmi1 -like genes are identified as possible candidates for leaf shape trait in cotton. leaf shape in cotton is an important trait that influences yield, flowering rates, disease resistance, lint trash, and the efficacy of foliar chemical application. the leaves of okra leaf cotton display a significantly ... | 2014 | 24158249 |
| ssr marker-assisted improvement of fiber qualities in gossypium hirsutum using g. barbadense introgression lines. | this study demonstrates the first practical use of csils for the transfer of fiber quality qtls into upland cotton cultivars using ssr markers without detrimentally affecting desirable agronomic characteristics. gossypium hirsutum is characterized by its high lint production and medium fiber quality compared to extra-long staple cotton g. barbadense. transferring valuable traits or genes from g. barbadense into g. hirsutum is a promising but challenging approach through a traditional interspecif ... | 2014 | 24306319 |
| transcriptome dynamics during fibre development in contrasting genotypes of gossypium hirsutum l. | understanding the contribution of genetic background in fibre quality traits is important for the development of future cotton varieties with superior fibre quality. we used affymetrix microarray (santa clara, ca) and roche 454 gsflx (branford, ct) for comparative transcriptome analysis between two superior and three inferior genotypes at six fibre developmental stages. microarray-based analysis of variance (anova) for 89 microarrays encompassing five contrasting genotypes and six developmental ... | 2014 | 24119257 |
| myb80 homologues in arabidopsis, cotton and brassica: regulation and functional conservation in tapetal and pollen development. | the arabidopsis atmyb80 transcription factor regulates genes involved in pollen development and controls the timing of tapetal programmed cell death (pcd). downregulation of atmyb80 expression precedes tapetal degradation. inhibition of atmyb80 expression results in complete male sterility. full-length atmyb80 homologs have been isolated in wheat, rice, barley and canola (c genome). | 2014 | 25311582 |
| ethnopharmacological assessment of medicinal plants used against livestock infections by the people living around indus river. | the present study was aimed to document detailed ethnopharmacological knowledge of medicinal plants against livestock infections of an unexplored remote region of pakistan. semistructured questionnaires were used for data collection. total 43 plants belonging to 26 families were found to be used in ethnoveterinary practices. seeds (29%) were found to be the most frequent plant part used followed by leaves (22%). ethnoveterinary recipes were mostly prepared in the form of decoction and powdering. ... | 2014 | 25544941 |
| in silico prediction of cotton (gossypium hirsutum) encoded micrornas targets in the genome of cotton leaf curl allahabad virus. | cotton leaf curl allahabad virus (clcuav) belongs to genus begomovirus, family geminiviridae. it has single stranded monopartite dna genome transmitted by whitefly (bemisia tabaci). micrornas (mirnas) belong to class of endogeneous small rnas which suppress expression of genes following cleavage or translational inhibition of target messenger rnas. they are demonstrated to be involved in a number of plant processes such as, development, biotic and abiotic stresses. employing in silico approach, ... | 2014 | 24966530 |
| ethnobotanical study of kani tribes in thoduhills of kerala, south india. | india is having a rich vegetation with a wide variety of plants, because of the extreme variations in geographical and climatic conditions prevailing in the country. plants have been used since ancient times for the treatment of various ailments. especially, kani tribal communities in thodu hills of kerala meet their healthcare needs by using non-timber minor forest products and preparations based on traditional knowledge. they still depend on medicinal plants and most of them have a basic knowl ... | 2014 | 24393788 |
| ghpsy, a phytoene synthase gene, is related to the red plant phenotype in upland cotton (gossypium hirsutum l.). | carotenoids are important accessory pigments in plants that are essential for photosynthesis. phytoene synthase (psy), a rate-controlling enzyme in the carotenoid biosynthesis pathway, has been widely characterized in rice, maize, and sorghum, but at present there are no reports describing this enzyme in cotton. in this study, ghpsy was identified as a candidate gene for the red plant phenotype via a combined strategy using: (1) molecular marker data for loci closely linked to r1; (2) the whole- ... | 2014 | 24718783 |
| upland cotton gene ghfpf1 confers promotion of flowering time and shade-avoidance responses in arabidopsis thaliana. | extensive studies on floral transition in model species have revealed a network of regulatory interactions between proteins that transduce and integrate developmental and environmental signals to promote or inhibit the transition to flowering. previous studies indicated flowering promoting factor 1 (fpf1) gene was involved in the promotion of flowering, but the molecular mechanism was still unclear. here, fpf1 homologous sequences were screened from diploid gossypium raimondii l. (d-genome, n = ... | 2014 | 24626476 |
| atwuschel promotes formation of the embryogenic callus in gossypium hirsutum. | upland cotton (gossypium hirsutum) is one of the most recalcitrant species for in vitro plant regeneration through somatic embryogenesis. callus from only a few cultivars can produce embryogenic callus (ec), but the mechanism is not well elucidated. here we screened a cultivar, cri24, with high efficiency of ec produce. the expression of genes relevant to ec production was analyzed between the materials easy to or difficult to produce ec. quantitative pcr showed that cri24, which had a 100% ec d ... | 2014 | 24498119 |
| sugar and auxin signaling pathways respond to high-temperature stress during anther development as revealed by transcript profiling analysis in cotton. | male reproduction in flowering plants is highly sensitive to high temperature (ht). to investigate molecular mechanisms of the response of cotton (gossypium hirsutum) anthers to ht, a relatively complete comparative transcriptome analysis was performed during anther development of cotton lines 84021 and h05 under normal temperature and ht conditions. in total, 4,599 differentially expressed genes were screened; the differentially expressed genes were mainly related to epigenetic modifications, c ... | 2014 | 24481135 |
| association between photosynthesis and contrasting features of minor veins in leaves of summer annuals loading phloem via symplastic versus apoplastic routes. | foliar vascular anatomy and photosynthesis were evaluated for a number of summer annual species that either load sugars into the phloem via a symplastic route (cucumis sativus l. cv. straight eight; cucurbita pepo l. cv. italian zucchini romanesco; citrullus lanatus l. cv. faerie hybrid; cucurbita pepo l. cv. autumn gold) or an apoplastic route (nicotiana tabacum l.; solanum lycopersicum l. cv. brandywine; gossypium hirsutum l.; helianthus annuus l. cv. soraya), as well as winter annual apoplast ... | 2014 | 24450755 |
| a study on the influence of different promoter and 5'utr (urm) cassettes from arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton. | several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. among these the camv 35s promoter is used extensively for transgene expression in plants. we identified candidate promoters from arabidopsis based on their transcript levels (meta-analysis of available microarray control datasets) to test their activity in comparison to the camv 35s promoter. a set of 11 candidate genes were identified which showed high transcript levels in t ... | 2014 | 24072400 |
| how does the host-specialized aphid deal with food deficiency? | aphis gossypii glover shows obvious host specialization, with cucurbit- and cotton-specialized biotypes or host races in many regions. because its annual natal host crops senesce earlier the cucurbit-specialized biotype may suffer food deficiency. the method this biotype uses to overcome this challenge is still poorly understood. in order to understand the potential of the cucurbit-specialized biotype aphids in host shift and usage, the performance of this biotype on cotton (gossypium hirsutum), ... | 2014 | 24526656 |
| pyramiding b genes in cotton achieves broader but not always higher resistance to bacterial blight. | near-isogenic lines of upland cotton (gossypium hirsutum) carrying single, race-specific genes b4, bin, and b7 for resistance to bacterial blight were used to develop a pyramid of lines with all possible combinations of two and three genes to learn whether the pyramid could achieve broad and high resistance approaching that of l. a. brinkerhoff's exceptional line im216. isogenic strains of xanthomonas axonopodis pv. malvacearum carrying single avirulence (avr) genes were used to identify plants ... | 2014 | 24655289 |
| overexpression of cotton ghmkk4 enhances disease susceptibility and affects abscisic acid, gibberellin and hydrogen peroxide signalling in transgenic nicotiana benthamiana. | mitogen-activated protein kinase (mapk) cascades are involved in plant development, stress responses and hormonal signal transduction. mapk kinases (mapkks), as the key nodes in these cascades, link mapks and mapkk kinases (mapkkks). in this study, ghmkk4, a novel group c mapkk gene from cotton (gossypium hirsutum), was isolated and identified. its expression can be induced by various stresses and signalling molecules. the overexpression of ghmkk4 in nicotiana benthamiana enhanced its susceptibi ... | 2014 | 23980654 |
| synthesis and evaluation of heterocyclic analogues of bromoxynil. | one attractive strategy to discover more active and/or crop-selective herbicides is to make structural changes to currently registered compounds. this strategy is especially appealing for those compounds with limited herbicide resistance and whose chemistry is accompanied with transgenic tools to enable herbicide tolerance in crop plants. bromoxynil is a photosystem ii (psii) inhibitor registered for control of broadleaf weeds in several agronomic and specialty crops. recently at the university ... | 2014 | 24354444 |
| the gossypium hirsutum wrky gene ghwrky39-1 promotes pathogen infection defense responses and mediates salt stress tolerance in transgenic nicotiana benthamiana. | our results indicate that overexpression of the ghwrky39 - 1 gene enhances resistance to pathogen infection and tolerance to high salt and oxidative stress in transgenic nicotiana benthamiana. | 2014 | 24337818 |
| re-evaluation of the inheritance for root-knot nematode resistance in the upland cotton germplasm line m-120 rnr revealed two epistatic qtls conferring resistance. | we report a second major qtl for root-knot nematode resistance in the highly resistant upland cotton line m-120rnr and show epistasis between two resistant qtls with different mechanisms conferring resistance. in an earlier study, we identified a major qtl on chromosome 11 associated with resistance to root-knot nematode in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source. herein, we re-evaluated the genetics of the resistance to root-knot nematode in the m-1 ... | 2014 | 24728014 |
| effect of simultaneous water deficit stress and meloidogyne incognita infection on cotton yield and fiber quality. | both water deficit stress and meloidogyne incognita infection can reduce cotton growth and yield, and drought can affect fiber quality, but the effect of nematodes on fiber quality is not well documented. to determine whether nematode parasitism affects fiber quality and whether the combined effects of nematode and drought stress on yield and quality are additive (independent effects), synergistic, or antagonistic, we conducted a study for 7 yr in a field infested with m. incognita. a split-plot ... | 2014 | 24987162 |
| physiological effects of meloidogyne incognita infection on cotton genotypes with differing levels of resistance in the greenhouse. | greenhouse tests were conducted to evaluate (i) the effect of meloidogyne incognita infection in cotton on plant growth and physiology including the height-to-node ratio, chlorophyll content, dark-adapted quantum yield of photosystem ii, and leaf area; and (ii) the extent to which moderate or high levels of resistance to m. incognita influenced these effects. cultivars fibermax 960 br (susceptible to m. incognita) and stoneville 5599 br (moderately resistant) were tested together in three trials ... | 2014 | 25580028 |
| site specific nematode management-development and success in cotton production in the united states. | variability in edaphic factors such as clay content, organic matter, and nutrient availability within individual fields is a major obstacle confronting cotton producers. adaptation of geospatial technologies such global positioning systems (gps), yield monitors, autosteering, and the automated on-and-off technology required for site-specific nematicide application has provided growers with additional tools for managing nematodes. multiple trials in several states were conducted to evaluate this ... | 2014 | 25580023 |
| conversion of fusaric acid to fusarinol by aspergillus tubingensis: a detoxification reaction. | the fungus fusarium oxysporum causes wilt diseases of plants and produces a potent phytotoxin fusaric acid (fa), which is also toxic to many microorganisms. an aspergillus tubingensis strain with high tolerance to fa was isolated from soil and designated as cdrat01. hplc analysis of culture filtrates from a. tubingensis isolate cdrat01 grown with the addition of fa indicated the formation of a metabolite over time that was associated with a decrease of fa. spectral analysis and chemical synthesi ... | 2014 | 24352475 |
| the entomopathogenic fungal endophytes purpureocillium lilacinum (formerly paecilomyces lilacinus) and beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions. | the effects of two entomopathogenic fungal endophytes, beauveria bassiana and purpureocillium lilacinum (formerly paecilomyces lilacinus), were assessed on the reproduction of cotton aphid, aphis gossypii glover (homoptera:aphididae), through in planta feeding trials. in replicate greenhouse and field trials, cotton plants (gossypium hirsutum) were inoculated as seed treatments with two concentrations of b. bassiana or p. lilacinum conidia. positive colonization of cotton by the endophytes was c ... | 2014 | 25093505 |
| using resistant prey demonstrates that bt plants producing cry1ac, cry2ab, and cry1f have no negative effects on geocoris punctipes and orius insidiosus. | geocoris punctipes (say) and orius insidiosus (say) are generalist predators found in a wide range of crops, including cotton (gossypium hirsutum l.) and maize (zea mays l.), where they provide important biological control services by feeding on an array of pests, including eggs and small larvae of caterpillars. a high percentage of cotton and maize in the united states and several other countries are transgenic cultivars that produce one or more of the insecticidal cry proteins of bacillus thur ... | 2014 | 24472212 |
| current status of genetic engineering in cotton (gossypium hirsutum l): an assessment. | cotton is considered as the foremost commercially important fiber crop and is deemed as the backbone of the textile industry. the productivity of cotton crop, worldwide, is severely hampered by the occurrence of pests, weeds, pathogens apart from various environmental factors. several beneficial agronomic traits, viz., early maturity, improved fiber quality, heat tolerance, etc. have been successfully incorporated into cotton varieties employing conventional hybridization and mutation breeding. ... | 2014 | 23190258 |
| a fungal protein elicitor pevd1 induces verticillium wilt resistance in cotton. | we found that the elicitor pevd1 triggered innate immunity in cotton, which plays an important role in future cotton wilt disease control. | 2014 | 24337817 |
| diversity of endophytic fungi from different verticillium-wilt-resistant gossypium hirsutum and evaluation of antifungal activity against verticillium dahliae in vitro. | cotton plants were sampled and ranked according to their resistance to verticillium wilt. in total, 642 endophytic fungi isolates representing 27 genera were recovered from gossypium hirsutum root, stem, and leaf tissues, but were not uniformly distributed. more endophytic fungi appeared in the leaf (391) compared with the root (140) and stem (111) sections. however, no significant difference in the abundance of isolated endophytes was found among resistant cotton varieties. alternaria exhibited ... | 2014 | 24836187 |
| characterization of developmental- and stress-mediated expression of cinnamoyl-coa reductase in kenaf (hibiscus cannabinus l.). | cinnamoyl-coa reductase (ccr) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. we have cloned a full length coding sequence of ccr from kenaf (hibiscus cannabinus l.), which contains a 1,020-bp open reading frame (orf), encoding 339 amino acids of 37.37 kda, with an isoelectric point (pi) of 6.27 (jx524276, hcccr2). blast result found that it has high homology with other plant ccr orthologs. multiple alignment with other ... | 2014 | 24723816 |
| cotton wrky1 mediates the plant defense-to-development transition during infection of cotton by verticillium dahliae by activating jasmonate zim-domain1 expression. | plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. previous research has demonstrated that della proteins physically interact with jasmonate zim-domain1 (jaz1) and dynamically regulate the interaction of the gibberellin (ga) and jasmonate (ja) signaling pathways. however, whether and how the jaz1-della regulatory node is regulated at the transcriptional level in plants under normal growth ... | 2014 | 25301887 |
| transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae. | cotton is a major fibre crop grown worldwide that suffers extensive damage from chewing insects, including the cotton boll weevil larvae (anthonomus grandis). transcriptome analysis was performed to understand the molecular interactions between gossypium hirsutum l. and cotton boll weevil larvae. the illumina hiseq 2000 platform was used to sequence the transcriptome of cotton flower buds infested with boll weevil larvae. | 2014 | 25280771 |
| ghwrky40, a multiple stress-responsive cotton wrky gene, plays an important role in the wounding response and enhances susceptibility to ralstonia solanacearum infection in transgenic nicotiana benthamiana. | wrky transcription factors form one of the largest transcription factor families and function as important components in the complex signaling processes that occur during plant stress responses. however, relative to the research progress in model plants, far less information is available on the function of wrky proteins in cotton. in the present study, we identified the ghwrky40 gene in cotton (gossypium hirsutum) and determined that the ghwrky40 protein is targeted to the nucleus and is a stres ... | 2014 | 24747610 |
| ghh2a12, a replication-dependent histone h2a gene from gossypium hirsutum, is negatively involved in the development of cotton fiber cells. | ghh2a12 was preferentially expressed at the initiation and early elongation stage of cotton fiber development, and overexpression of ghh2a12 caused retardation of fiber initiation and produced shorter fibers. histone h2a is a component of eukaryotic chromatin whose function has not been studied in cotton. we have isolated an h2a gene encoding 156 amino acids, named ghh2a12. like other plant histone h2as, ghh2a12 contains a typical spkk motif in the carboxy-terminal and a plant-unique peptide-bin ... | 2014 | 25001001 |
| high throughput agrobacterium tumefaciens-mediated germline transformation of mechanically isolated meristem explants of cotton (gossypium hirsutum l.). | agrobacterium tumefaciens mediates high frequency of germline transformation of cotton meristem explants. the meristem transformation system we developed is rapid, high throughput and genotype-flexible. we have developed a high throughput cotton transformation system based on direct agrobacterium inoculation of mechanically isolated meristem explants of cotton (gossypium hirsutum l.). the explants were inoculated with a disarmed a. tumefaciens strain, ab33 harboring a 2 t-dna binary vector pmon1 ... | 2014 | 24129847 |
| virus-induced gene silencing using a modified betasatellite: a potential candidate for functional genomics of crops. | betasatellites are geminivirus-associated single-stranded dna molecules that play an important role in symptom modulation. a vigs vector was developed by modifying cotton leaf curl multan betasatellite (clcumb). clcumb dna was modified by replacing the βc1 gene with a multiple cloning site. the silencing ability of the modified clcumb was investigated by cloning a fragment of a host gene (su) or a reporter transgene (uida) into the modified clcumb and co-agroinoculation with cotton leaf curl mul ... | 2014 | 24610555 |
| mosquito larvicidal potential of gossypium hirsutum (bt cotton) leaves extracts against aedes aegypti and anopheles stephensi larvae. | we aimed to extract the ingredients from leaves of gossypium hirsutum (bt cotton) using different solvents and evaluate for potential use to control different larval stages of mosquito species, aedes aegypti and anopheles stephensi. | 2013 | 25629069 |
| agroinoculation of a full-length cdna clone of cotton leafroll dwarf virus (clrdv) results in systemic infection in cotton and the model plant nicotiana benthamiana. | cotton blue disease is the most important viral disease of cotton in the southern part of south america. its etiological agent, cotton leafroll dwarf virus (clrdv), is specifically transmitted to host plants by the aphid vector (aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. this limitation has held back the study of this virus and the disease it causes. in this study, a full-length cdna of clrdv was constructed and expressed in vivo un ... | 2013 | 23623981 |
| development of insect-resistant transgenic cotton with chimeric tvip3a accumulating in chloroplasts. | an optimized vip3a gene, designated as vip3a* was chemically synthesized and a thi1 gene chloroplast transit peptide coding sequence was attached to its 5' end to produce the tvip3a*. vip3a* and tvip3a* genes were transformed into gossypium hirsutum cv. zhongmiansuo35 mediated by agrobacterium tumefaciens. four independent transgenic t1 lines with single-copy insertions and unchanged phenotypes (ctv1 and ctv2 for tvip3a*, and cv1 and cv2 for vip3a*) were selected by polymerase chain reaction (pc ... | 2013 | 23143498 |
| comparative transcriptome analysis of gossypium hirsutum l. in response to sap sucking insects: aphid and whitefly. | cotton (gossypium hirsutum l.) is a major fiber crop that is grown worldwide; it faces extensive damage from sap-sucking insects, including aphids and whiteflies. genome-wide transcriptome analysis was performed to understand the molecular details of interaction between gossypium hirsutum l. and sap-sucking pests, namely aphis gossypii (aphid) and bemisia tabacci (whiteflies). roche's gs-titanium was used to sequence transcriptomes of cotton infested with aphids and whiteflies for 2 h and 24 h. | 2013 | 23577705 |
| coordinate accumulation of homeologous transcripts of seven cotton lea gene families during embryogenesis and germination. | one of two related patterns of total transcript accumulation are seen during embryogenesis for 18 cotton lea (late embryogenesis-abundant) gene families in the allotetraploid cotton gossypium hirsutum l. cv coker 201. coordinate accumulation in each class is complex, suggesting that lea mrna abundance is regulated by several events. each of the lea gene families probably contains two active homeologous genes (alloalleles), one in each of cotton's two subgenomes. it is of interest whether both tr ... | 2013 | 3622929 |
| cotton ghmkk1 induces the tolerance of salt and drought stress, and mediates defence responses to pathogen infection in transgenic nicotiana benthamiana. | mitogen-activated protein kinase kinases (mapkk) mediate a variety of stress responses in plants. so far little is known on the functional role of mapkks in cotton. in the present study, gossypium hirsutum mkk1 (ghmkk1) function was investigated. ghmkk1 protein may activate its specific targets in both the nucleus and cytoplasm. treatments with salt, drought, and h2o2 induced the expression of ghmkk1 and increased the activity of ghmkk1, while overexpression of ghmkk1 in nicotiana benthamiana en ... | 2013 | 23844212 |
| construction of a plant-transformation-competent bibac library and genome sequence analysis of polyploid upland cotton (gossypium hirsutum l.). | cotton, one of the world's leading crops, is important to the world's textile and energy industries, and is a model species for studies of plant polyploidization, cellulose biosynthesis and cell wall biogenesis. here, we report the construction of a plant-transformation-competent binary bacterial artificial chromosome (bibac) library and comparative genome sequence analysis of polyploid upland cotton (gossypium hirsutum l.) with one of its diploid putative progenitor species, g. raimondii ulbr. | 2013 | 23537070 |
| a transcript profiling approach reveals an abscisic acid-specific glycosyltransferase (ugt73c14) induced in developing fiber of ligon lintless-2 mutant of cotton (gossypium hirsutum l.). | ligon lintless-2, a monogenic dominant cotton (gossypium hirsutum l.) fiber mutation, causing extreme reduction in lint fiber length with no pleiotropic effects on vegetative growth, represents an excellent model system to study fiber elongation. a udp-glycosyltransferase that was highly expressed in developing fibers of the mutant ligon lintless-2 was isolated. the predicted amino acid sequence showed ~53% similarity with arabidopsis ugt73c sub-family members and the udp-glycosyltransferase was ... | 2013 | 24086489 |
| spatial and temporal variation in fungal endophyte communities isolated from cultivated cotton (gossypium hirsutum). | studies of fungi in upland cotton (gossypium hirsutum) cultivated in the united states have largely focused on monitoring and controlling plant pathogens. given increasing interest in asymptomatic fungal endophytes as potential biological control agents, surveys are needed to better characterize their diversity, distribution patterns and possible applications in integrated pest management. we sampled multiple varieties of cotton in texas, usa and tested for temporal and spatial variation in fung ... | 2013 | 23776604 |
| overexpression of ft in cotton affects architecture but not floral organogenesis. | flowering marks the change from indeterminate to determinate plant growth, and this developmental transition involves the activity of the arabidopsis flowering locus t (ft) gene product and its orthologs. we demonstrated that when ft is ectopically expressed from a viral vector in cotton, a process referred to as virus induced flowering (vif), it uncouples flowering from photoperiodic regulation and promotes determinate growth in aerial organs. the accelerated switch to determinate growth affect ... | 2013 | 23333977 |
| the relationship between environmental variables and response of cotton to nematicides. | nematicide/irrigation rate trials were conducted in texas (tx) in 2009 and 2010 in cotton grown at three irrigation rates, where irrigation rate (base (b), b - 33%, b + 33%) was the main plot and treatment (untreated check, aldicarb, and nematicide seed treatment (nst) and nst + aldicarb) were the subplots. aldicarb improved cotton lint yield with the base (medium) irrigation rate over the untreated check, but not at the b - 33% and b + 33% irrigation rates. in a second evaluation, 20 tests cond ... | 2013 | 23589654 |
| cotton ghmpk6a negatively regulates osmotic tolerance and bacterial infection in transgenic nicotiana benthamiana, and plays a pivotal role in development. | mitogen-activated protein kinase (mapk) cascades play important roles in the perception of external signals and the generation of suitable responses. cotton (gossypium hirsutum) is an important fibre-producing and oil-producing crop worldwide. however, few mapks and their interaction partners have been functionally characterized in cotton. in the present study, the group a mapk g. hirsutum (gh)mpk6a was identified and characterized. ghmpk6a expression can be induced through multiple defence-rela ... | 2013 | 23957843 |
| the effect of soil texture and irrigation on rotylenchulus reniformis and cotton. | the reniform nematode, rotylenchulus reniformis, is the most damaging nematode pathogen of cotton in alabama. soil texture is currently being explored as a basis for the development of economic thresholds and management zones within a field. trials to determine the reproductive potential of r. reniformis as influenced by soil type were conducted in microplot and greenhouse settings during 2008 to 2010. population density of r. reniformis was significantly influenced by soil texture and exhibited ... | 2013 | 23833324 |
| development of agrobacterium-mediated virus-induced gene silencing and performance evaluation of four marker genes in gossypium barbadense. | gossypiumbarbadense is a cultivated cotton species and possesses many desirable traits, including high fiber quality and resistance to pathogens, especially verticilliumdahliae (a devastating pathogen of gossypium hirsutum, the main cultivated species). these elite traits are difficult to be introduced into g. hirsutum through classical breeding methods. in addition, genetic transformation of g. barbadense has not been successfully performed. it is therefore important to develop methods for eval ... | 2013 | 24023833 |
| ghcax3 gene, a novel ca(2+)/h(+) exchanger from cotton, confers regulation of cold response and aba induced signal transduction. | as a second messenger, ca(2+) plays a major role in cold induced transduction via stimulus-specific increases in [ca(2+)]cyt, which is called calcium signature. during this process, caxs (ca(2+)/h(+) exchangers) play critical role. for the first time, a putative ca(2+)/h(+) exchanger ghcax3 gene from upland cotton (gossypium hirsutum cv. 'yz-1') was isolated and characterized. it was highly expressed in all tissues of cotton except roots and fibers. this gene may act as a regulator in cotton's r ... | 2013 | 23776653 |
| cloning and functional analysis of a novel ascorbate peroxidase (apx) gene from anthurium andraeanum. | an 888-bp full-length ascorbate peroxidase (apx) complementary dna (cdna) gene was cloned from anthurium andraeanum, and designated as anapx. it contains a 110-bp 5'-noncoding region, a 28-bp 3'-noncoding region, and a 750-bp open reading frame (orf). this protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting of α-helixes, β-turns, and random coils. the anapx protein showed 93%, 87%, 87%, 87%, and 86% similarities to the apx homologs from zantedeschia aethiopica, v ... | 2013 | 24302711 |
| the cotton transcription factor tcp14 functions in auxin-mediated epidermal cell differentiation and elongation. | plant-specific teosinte-branched1/cycloidea/pcf (tcp) transcription factors play crucial roles in development, but their functional mechanisms remain largely unknown. here, we characterized the cellular functions of the class i tcp transcription factor ghtcp14 from upland cotton (gossypium hirsutum). ghtcp14 is expressed predominantly in fiber cells, especially at the initiation and elongation stages of development, and its expression increased in response to exogenous auxin. induced heterologou ... | 2013 | 23715527 |
| wuschel overexpression promotes somatic embryogenesis and induces organogenesis in cotton (gossypium hirsutum l.) tissues cultured in vitro. | this work shows that overexpression of the wus gene from arabidopsis enhanced the expression of embryogenic competence and triggered organogenesis from some cells of the regenerated embryo-like structures. agrobacterium-mediated genetic transformation of cotton was described in the late 1980s, but is still time consuming and largely genotype dependant due to poor regeneration. to help solve this bottleneck, we over-expressed the wuschel (wus) gene, a homeobox transcription factor cloned in arabi ... | 2013 | 23543366 |
| genetic transformation of cotton with a harpin-encoding gene hpaxoo confers an enhanced defense response against verticillium dahliae kleb. | the soil-borne fungal pathogen verticillium dahliae kleb causes verticillium wilt in a wide range of crops including cotton (gossypium hirsutum). to date, most upland cotton varieties are susceptible to v. dahliae and the breeding for cotton varieties with the resistance to verticillium wilt has not been successful. hpa1xoo is a harpin protein from xanthomonas oryzae pv. oryzae which induces the hypersensitive cell death in plants. when hpa1xoo was transformed into the susceptible cotton line z3 ... | 2013 | 23143497 |
| overexpression of a cotton gene that encodes a putative transcription factor of ap2/erebp family in arabidopsis affects growth and development of transgenic plants. | in the study, a gene encoding a putative ethylene response factor of ap2/erebp family was isolated from cotton (gossypium hirsutum) and designated as gherf12. sequence alignment showed that gherf12 protein contains a central ap2/erf domain (58 amino acids) with two functional conserved amino acid residues (ala14 and asp19). transactivation assay indicated that gherf12 displayed strong transcription activation activity in yeast cells, suggesting that this protein may be a transcriptional activato ... | 2013 | 24194949 |
| cotton plasma membrane intrinsic protein 2s (pip2s) selectively interact to regulate their water channel activities and are required for fibre development. | aquaporins are thought to be associated with water transport and play important roles in cotton (gossypium hirsutum) fibre elongation. among aquaporins, plasma membrane intrinsic proteins (pips) constitute a plasma-membrane-specific subfamily and are further subdivided into pip1 and pip2 groups. in this study, four fibre-preferential ghpip2 genes were functionally characterized. the selective interactions among ghpip2s and their interaction proteins were studied in detail to elucidate the molecu ... | 2013 | 23656428 |
| a cotton gene encoding myb-like transcription factor is specifically expressed in pollen and is involved in regulation of late anther/pollen development. | in flowering plants, pollen development is a highly programmed process, in which a lot of genes are involved. in this study, a gene, designated as ghmyb24, encoding r2r3-myb-like protein was isolated from cotton. ghmyb24 protein is localized in the cell nucleus and acts as a transcriptional activator. northern blot analysis revealed that ghmyb24 transcripts were predominantly detected in anthers. it was further found that strong expression of ghmyb24 was mainly detected in pollen and was regulat ... | 2013 | 23447105 |
| a cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress. | cotton (gossypium hirsutum), the most important textile crop worldwide, often encounters abiotic stress such as drought and waterlog during its growth season (summer), and its productivity is significantly limited by adverse factors. to investigate the molecular adaptation mechanisms of this plant species to abiotic stress, a gene encoding the plasma membrane intrinsic protein (pip) was isolated in cotton, and designated as ghpip2;7. quantitative reverse transcriptase polymerase chain reaction a ... | 2013 | 23178915 |
| likelihood of stink bugs colonizing crops: a case study in southeastern farmscapes. | stink bugs, including nezara viridula (l.), euschistus servus (say), and chinavia hilaris (say), are economic pests across agricultural farmscapes where they can colonize closely associated crops. this 4-yr on-farm study was conducted to examine the likelihood of these three stink bug species colonizing crops in corn-cotton, corn-peanut-cotton, and peanut-cotton farmscapes by using odds ratios. corn (zea mays l.), peanut (arachis hypogaea l.), and cotton (gossypium hirsutum l.) served as host pl ... | 2013 | 23726052 |
| analyzing blends of herbivore-induced volatile organic compounds with factor analysis: revisiting "cotton plant, gossypium hirsutum l., defense in response to nitrogen fertilization". | many herbivorous, predaceous, and parasitic insects use constitutive and herbivore-induced volatile organic compounds (vocs) to locate their respective host plant, prey, and hosts. multivariate statistical tools (e.g., factor analysis) are recognized increasingly as an appropriate approach for analyzing intercorrelated data such as presence/absence or quantities of vocs. one challenge of implementing factor analysis is determining how many new variables (factors) to retain in the final analysis. ... | 2013 | 23786100 |
| bac-pool sequencing and analysis of large segments of a12 and d12 homoeologous chromosomes in upland cotton. | although new and emerging next-generation sequencing (ngs) technologies have reduced sequencing costs significantly, much work remains to implement them for de novo sequencing of complex and highly repetitive genomes such as the tetraploid genome of upland cotton (gossypium hirsutum l.). herein we report the results from implementing a novel, hybrid sanger/454-based bac-pool sequencing strategy using minimum tiling path (mtp) bacs from ctg-3301 and ctg-465, two large genomic segments in a12 and ... | 2013 | 24116150 |
| high-frequency regeneration via multiple shoot induction of an elite recalcitrant cotton (gossypium hirsutum l. cv narashima) by using embryo apex. | cotton (gossypium hirsutum l.) is one of the most commercially important fiber crops in the world. compared with other crops, cotton represents a recalcitrant species for regeneration protocols. the development of efficient and rapid regeneration protocol for elite indian cotton variety could help improve the quality characteristics and biotic or abiotic stress tolerance. here we report a novel regeneration protocol in indian cotton cultivar narashima. the maximum number of multiple shoots obtai ... | 2013 | 23221745 |