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an in vivo, label-free quick assay for xylose transport in escherichia coli.efficient use of xylose is necessary for economic production of biochemicals and biofuels from lignocellulosic materials. current studies on xylose uptake for various microorganisms have been hampered by the lack of a facile assay for xylose transport. in this work, a rapid in vivo, label-free method for measuring xylose transport in escherichia coli was developed by taking advantage of the bacillus pumilus xylosidase (xynb), which cleaved a commercially available xylose analog, p-nitrophenyl-be ...200919351525
acetoin catabolism and acetylbutanediol formation by bacillus pumilus in a chemically defined medium.most low molecular diols are highly water-soluble, hygroscopic, and reactive with many organic compounds. in the past decades, microbial research to produce diols, e.g. 1,3-propanediol and 2,3-butanediol, were considerably expanded due to their versatile usages especially in polymer synthesis and as possible alternatives to fossil based feedstocks from the bioconversion of renewable natural resources. this study aimed to provide a new way for bacterial production of an acetylated diol, i.e. acet ...200919461961
studies on the biocidal and cell membrane disruption potentials of stem bark extracts of afzelia africana (smith).we had recently reported antibacterial activity in the crude extract of the stem bark of afzelia africana (akinpelu et al., 2008). in this study, we assessed the biocidal and cell membrane disruption potentials of fractions obtained from the crude extract of the plant. the aqueous (aq) and butanol (bl) fractions exhibited appreciable antibacterial activities against the test bacteria. the minimum inhibitory concentrations of the aq and bl fractions ranged between 0.313 and 2.5 mg/ml, while their ...200919915742
synthesis and antibacterial activity of some new 2,3-dimethoxy-3-hydroxy-2-(1-phenyl-3-aryl-4-pyrazolyl)chromanones.seven new 2,3-dimethoxy-3-hydroxy-2-(1-phenyl-3-aryl-4-pyrazolyl)chromanones (5) have been synthesized by the oxidation of 3-hydroxy-2-(1-phenyl-3-aryl-4-pyrazolyl)chromones (4) with iodobenzene diacetate in methanol. the structures of compounds 5 were established by the combined use of (1)h nmr, ir and mass spectra. all the seven compounds (5) were tested in vitro for their antibacterial activity against gram-positive bacteria namely, staphylococcus aureus, staphylococcus epidermidis and bacill ...200918504062
sequential anaerobic-aerobic degradation of munitions waste.a sequential anaerobic-aerobic biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) was studied. the results demonstrated that: (i) a complete degradation of rdx was achieved within 20 days using a consortium of bacteria from a wastewater activated sludge, (ii) rdx degradation did not occur under aerobic conditions alone, (iii) rdx-degrading bacterial strain that was isolated from the activated sludge completely degraded rdx within 2 days, and (iv) rdx- induced protein expressions wer ...200918779925
megacell phenotype and its relation to metabolic alterations in transketolase deficient strain of bacillus pumilus.fermentation with transketolase (tkt) deficient strain of bacillus is the only reported industrially viable process for production of d-ribose, a commercially important pentose sugar. in addition to direct effects of tkt deficiency, the mutation in non-oxidative part of pentose phosphate pathway (ppp) is known to display several unexpected physiological characteristics such as decreased ability to utilize d-glucose, altered carbon catabolite repression, lack of motility, etc. here we demonstrate ...200919039788
biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by bacillus pumilus strain c2a1.a bacterial strain c2a1 isolated from soil was found highly effective in degrading chlorpyrifos and its first hydrolysis metabolite 3,5,6-trichloro-2-pyridinol (tcp). on the basis of morphology, physiological characteristics, biochemical tests and 16s rrna sequence analysis, strain c2a1 was identified as bacillus pumilus. role of strain c2a1 in the degradation of chlorpyrifos was examined under different culture conditions like ph, inoculum density, presence of added carbon/nutrient sources and ...200919297093
overexpression, purification and characterization of organic solvent stable lipase from bacillus licheniformis rsp-09.the lipase gene (543 bp) from bacillus licheniformis rsp-09, a thermophilic isolate, was overexpressed in escherichia coli bl21 (de3). it encodes a polypeptide of 181 residues and has 96% identity with bacillus pumilus b26 lipase gene. the recombinant lipase was purified 19-fold to electrophoretic homogeneity by his-tag chromatography. the molecular mass of the purified recombinant b. licheniformis rsp-09 lipase was found to be 24 kda. the purified recombinant b. licheniformis rsp-09 lipase exhi ...200919270444
substrate specificity and thermostability of the dehairing alkaline protease from bacillus pumilus.an alkaline protease (dhap) from bacillus pumilus has shown great potential in hide dehairing. to get better insights on its catalytic properties for application, the substrate specificity and thermostability were investigated using five natural proteins and nine synthetic peptides. the results showed that dhap could hydrolyze five proteins tested here in different specificity. collagen, a component of animal skin, was more resistant to hydrolysis than casein, fibrin, and gelatin. among the synt ...200919132554
isolation of a novel strain of bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens.a bacterium was isolated from the mid-gut of healthy black tiger shrimp, penaeus monodon, based on a large zone of inhibition in mixed culture on solid medium. the isolate was a gram-positive, motile spore former, with an optimum ph range for growth in tryptone soya broth containing 2% nacl of between ph 6 and 9. the bacterium was highly salt tolerant with concentrations between 0% and 8% having no detrimental effect on growth. the isolate was identified as bacillus pumilus based on physiologica ...200919573134
large-scale degumming of ramie fibre using a newly isolated bacillus pumilus dks1 with high pectate lyase activity.a combined (enzymatic and chemical) process using a bacillus pumilus strain (dks1), isolated from the soil, was used to degum ramie bast fibres. after 24 h of incubation with the isolated pectinolytic strain using a low-cost medium, the weight loss of the ramie fibre was found to be 25% under small scale. high activity of pectate lyase was detected in the culture supernatants; 400 kg of ramie fibres was degummed with 24% weight loss in large-scale degumming under field conditions. no cellulase a ...200918956216
plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers.the search for microorganisms that improve soil fertility and enhance plant nutrition has continued to attract attention due to the increasing cost of fertilizers and some of their negative environmental impacts. the objectives of this greenhouse study with tomato were to determine (1) if reduced rates of inorganic fertilizer coupled with microbial inoculants will produce plant growth, yield, and nutrient uptake levels equivalent to those with full rates of the fertilizer and (2) the minimum lev ...200919466478
metalloantibiotics: synthesis, characterization and antimicrobial evaluation of bismuth-fluoroquinolone complexes against helicobacter pylori.novel organometallic compounds have been prepared by complexing the fluoroquinolones, norfloxacin, ofloxacin, ciprofloxacin, sparfloxacin, lomefloxacin, pefloxacin and gatifloxacin, with bismuth. the complexes were characterized by uv, ir, atomic absorption spectroscopy, elemental analysis, differential scanning calorimetry, thermogravimetric analysis and mass spectrometry. their antibacterial potential against helicobacter pylori and other microorganisms was investigated. these compounds were f ...200919819823
purification and partial characterization of manganese peroxidase from bacillus pumilus and paenibacillus sp.the production of manganese peroxidase (mnp) from bacillus pumilus and paenibacillus sp. was studied under absence and presence of the inducers indulin at, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. indulin at increased the activity of b. pumilus mnp up to 31.66 u/l after 8 h, but no improve was observed for paenibacillus sp., which reached maximum activity (12.22 u/l) after 20 h. both mnps produced by these microorganisms were purified in phenyl sepharos ...200924031429
use of bacillus pumilus cbmai 0008 and paenibacillus sp. cbmai 868 for colour removal from paper mill effluent.bacillus pumilus and paenibacillus sp. were applied on the paper mill effluent to investigate the colour remotion. inocula were individually applied in effluent at ph 7.0, 9.0 and 11.0. the real colour and cod remotion after 48h at ph 9.0 were, respectively, 41.87% and 22.08% for b. pumilus treatment and 42.30% and 22.89% for paenibacillus sp. gel permeation chromatography was used to verify the molar masses of compounds in the non-treated and treated effluent, showing a decrease in the compound ...200924031372
biochemical and molecular characterization of bacillus pumilus isolated from coastal environment in cochin, india.bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. in this study, bacillus strains isolated from the coastal environment of cochin, india were identified by detailed conventional biochemical methods, fatty acid methyl ester (fame) analysis and partial 16s rdna sequencing. analysis of the data revealed that bacillus pumilus was the most predominant species in the region under study followed by b. cereus and b. sphaericus. the b. pumilus ...200924031357
preliminary phytochemical screening and antimicrobial studies on artocarpus lakoocha roxb.the antibacterial activity of artocarpus lakoocha roxb. bark was tested against different species of bacteria. the minimum inhibitory concentration (mic) of the methanolic extract showed that there was no growth in shigella soneii 2, e.coli mtcc 1568, bacillus pumilus 8241, proteus mirabilis am 198, bacillus subtelis atcc 6633, e. coli row 7/12 species at 200-400g/ml. from the result obtained, it was observed that the methanolic extract of. artocarpus lakoocha at concentration of 200 gl ml and 4 ...200922557328
production and accumulation of xylooligosaccharides with long chains by growing culture and xylanase of a mutant strain of bacillus pumilus x-6-19.bacillus pumilus x-6-9 isolated from soil and subsequently identified, produced xylooligosaccharides with long chains from xylan and accumulated them in the culture. by improving the culture conditions and mutating the bacterium, a 3.2-fold increase in the production of the xylooligosaccharides was established, when compared to the original culture conditions of b. pumilus x-6-19. the addition of d-glucose to the culture of the mutant strain u-3 of b. pumilus x-6-9 repressed the synthesis of bet ...200818837399
production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus eb3.in this study, endoglucanase was produced from oil palm empty fruit bunch (opefb) by a locally isolated aerobic bacterium, bacillus pumilus eb3. the effects of the fermentation parameters such as initial ph, temperature, and nitrogen source on the endoglucanase production were studied using carboxymethyl cellulose (cmc) as the carbon source. endoglucanase from b. pumilus eb3 was maximally secreted at 37 degrees c, initial ph 7.0 with 10 g/l of cmc as carbon source, and 2 g/l of yeast extract as ...200818929997
[directed evolution of lipase of bacillus pumilus yz02 by error-prone pcr].random mutagenesis on bacillus pumilus lipase yz02 gene was conducted by using error-prone pcr strategy. through two cycles of directed evolution, two optimum mutants bpl1-7 and bpl2-1369 with lipase activity improved 2 folds and 6 folds respectively were screened. the sequence of bpl2-1369 lipase gene showed that four nucleotides substitution, t61c, c147t, a334g and t371a have occurred, and three of them caused amino acid changes. thus, amine acid ser21 was changed into pro21, arg112 to gly112, ...200818589821
antibacterial stilbenoids from the roots of stemona tuberosa.twelve dihydrostilbenes, stilbostemins n-y (1-12), and a phenanthraquinone, stemanthraquinone (13), were isolated and identified from roots of stemona tuberosa, along with five known dihydrostilbenes. their structures were established on the basis of 1d and 2d nmr and other spectroscopic analyses. dihydrostilbene 8 exhibited strong activity against bacillus pumilus (mit 12.5-25 microg/ml). many tested compounds exhibited moderate antibacterial activities.200817826806
[two cases of bacillus infection and immunodepression].members of the genus bacillus are gram-positive bacilli, ubiquitous in the environment. when isolated in clinical practice, it is frequently considered as due to environmental contamination. bacillus cereus is the most frequent species isolated in clinical practice, nevertheless other bacillus spp. are sometimes isolated. bacillus bacteremia is uncommon, the affected patients are severely ill and frequently immunocompromised with hematological malignancies.200818976873
antibacterial activity of the fruits of iranian torilis leptophylla against some clinical pathogens.the aim of this study was to examine the antimicrobial activity of the methanolic extract of torilis leptophylla was tested on eleven bacteria (bacillus anthracis, bacillus subtilis, bacillus pumilus, staphylococcus aureus, bacillus licheniformis, brucella melitensis, escherichia coli, salmonella typhi, proteus mirabilis, bordetella bronshiseptica and pseudomonas aeruginosa). tested extract was effective against all bacteria but not b. subtilis. consequently, the ethanolic extract had antibacter ...200818819541
isolation and identification of a lipase producing bacillus sp. from soil.lipase production in an indigenous lipolytic bacillus sp. was detected in media containing tributyrin-tween 80 and rhodamine b-olive oil. the statistical taguchi model was used to predict the optimum experimental conditions for bacterial growth and lipase production. partial optimization was carried out for selection of salt base, oil, glucose, nh4cl and yeast extract concentrations, inoculum density, ph and agitation. maximum lipase activity was detected in the cell free supernatants of culture ...200818819570
comparative analysis of the diversity of aerobic spore-forming bacteria in raw milk from organic and conventional dairy farms.bacterial contamination of raw milk can originate from different sources: air, milking equipment, feed, soil, faeces and grass. it is hypothesized that differences in feeding and housing strategies of cows may influence the microbial quality of milk. this assumption was investigated through comparison of the aerobic spore-forming flora in milk from organic and conventional dairy farms. laboratory pasteurized milk samples from five conventional and five organic dairy farms, sampled in late summer ...200818406093
ribosomal rna-based analysis of the bacterial flora from the conjunctivae of cattle with bovine keratoconjunctivitis (bkc).bovine keratoconjunctivitis (bkc), colloquially referred to as 'pinkeye', is a disease affecting cattle worldwide; it costs cattle producers millions of dollars in economic loss annually. while moraxella spp. are the primary etiologic agent of pinkeye, surveys of flora from the conjunctivae of livestock from around the world have indicated that a variety of bacterial commensals occupy this niche. we used molecular biology-based methods to determine the composition of bacterial flora in the conju ...200818513895
persistence of biomarker atp and atp-generating capability in bacterial cells and spores contaminating spacecraft materials under earth conditions and in a simulated martian environment.most planetary protection research has concentrated on characterizing viable bioloads on spacecraft surfaces, developing techniques for bioload reduction prior to launch, and studying the effects of simulated martian environments on microbial survival. little research has examined the persistence of biogenic signature molecules on spacecraft materials under simulated martian surface conditions. this study examined how endogenous adenosine-5'-triphosphate (atp) would persist on aluminum coupons u ...200818567687
thermodynamic characterization of a highly thermoactive extracellular pectate lyase from a new isolate bacillus pumilus dks1.an extracellular pectate lyase (ec 4.2.2.2) was purified from the culture filtrate of a newly isolated bacillus pumilus dks1 grown in pectin containing medium. using ion-exchange and gel filtration chromatography, this enzyme was purified and found to have a molecular weight of around 35kda. the purified enzyme exhibited maximal activity at a temperature of 75 degrees c and ph 8.5. the presence of 1mm calcium and manganese enhanced pectate lyase activity and was strongly inhibited by zinc, nicke ...200818445524
enterotoxins and emetic toxins production by bacillus cereus and other species of bacillus isolated from soumbala and bikalga, african alkaline fermented food condiments.the ability of various species of bacillus from fermented seeds of parkia biglobosa known as african locust bean (soumbala) and fermented seeds of hibiscus sabdariffa (bikalga) was investigated. the study included screening of the isolates by haemolysis on blood agar, detection of toxins in broth and during the fermentation of african locust bean using the bacillus cereus enterotoxin reverse passive latex agglutination test kit (bcet-rpla) and the bacillus diarrhoeal enterotoxin visual immunoass ...200818474404
detoxification of toxic heavy metals by marine bacteria highly resistant to mercury.pollution in industrial areas is a serious environmental concern, and interest in bacterial resistance to heavy metals is of practical significance. mercury (hg), cadmium (cd), and lead (pb) are known to cause damage to living organisms, including human beings. several marine bacteria highly resistant to mercury (bhrm) capable of growing at 25 ppm (mg l(-1)) or higher concentrations of mercury were tested during this study to evaluate their potential to detoxify cd and pb. results indicate their ...200818288535
radiation sterilisation of doxorubicin bound to poly(butyl cyanoacrylate) nanoparticles.doxorubicin-loaded poly(butyl cyanoacrylate) (pbca) nanoparticles were prepared by anionic polymerisation under non-aseptic conditions. the feasibility of sterilisation of this formulation using either gamma-irradiation or electron beam irradiation was investigated. the irradiation doses ranged from 10 to 35 kgy. bacillus pumilus was used as the official test microorganism. the bioburden of the untreated formulation was found to be 100 cfu/g. microbiological monitoring revealed that at this leve ...200818295418
experimental and qsar studies on antimicrobial activity of benzimidazole derivatives.twenty eight analogues of benzimidazoles had been synthesized and tested for their antimicrobial activity against four bacteria (staphylococcus auerus, escherichia coli, bacillus pumilus and proteus vulgaris) and two fungi (aspergillus flavus and aspergilus niger). compounds with r as c6h4no2 and r' as so2c6h4-ch3(p), with r as c6h4och3 and r' as so2c6h4-ch3(p), and with r as ch2c6h5 and r' as ch2(ch2)9cl exhibited comparable or higher antibacterial activity than ciprofloxacin against s. auerus ...200818310935
action of antimicrobial substances produced by different oil reservoir bacillus strains against biofilm formation.microbial colonization of petroleum industry systems takes place through the formation of biofilms, and can result in biodeterioration of the metal surfaces. in a previous study, two oil reservoir bacillus strains (bacillus licheniformis t6-5 and bacillus firmus h(2)o-1) were shown to produce antimicrobial substances (ams) active against different bacillus strains and a consortium of sulfate-reducing bacteria (srb) on solid medium. however, neither their ability to form biofilms nor the effect o ...200818330565
isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities.this study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell anadara broughtoni inhabiting in the sea of japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. a total of 149 strains were isolated and identified phenotypically. a total of 27 strains were selected to be investigated phylogenetically by 165 rrna gene sequence analysis. the most bacteria were aff ...200819216104
[in vitro studies on antioxidant and antimicrobial activities of polysaccharide from lycoris aurea].to study the preliminary antioxidant and antimicrobial activities of polysaccharide extracted from lycoris aurea.200819230410
biodegradation of phenanthrene and pyrene from mangrove sediment in subtropical taiwan.this study investigated the effects of various factors on the biodegradation of the polycyclic aromatic hydrocarbons (pahs) phenanthrene and pyrene, and characterized the structure of the microbial community in mangrove sediment from subtropical taiwan. the degradation of pah was enhanced by the addition of brij 30, tween 80, yeast extract, hydrogen peroxide, sodium chloride or cellulose. however, pah degradation was inhibited by the addition of humic acid, di-(2-ethylhexyl), phthalate (dehp) or ...200818205053
biochemical and molecular characterization of a detergent-stable serine alkaline protease from bacillus pumilus cbs with high catalytic efficiency.we have described previously the potential use of an alkaline protease from bacillus pumilus cbs as an effective additive in laundry detergent formulations [b. jaouadi, s. ellouz-chaabouni, m. ben ali, e. ben messaoud, b. naili, a. dhouib, s. bejar, a novel alkaline protease from bacillus pumilus cbs having a high compatibility with laundry detergent and a high feather-degrading activity, process biochem, submitted for publication]. here, we purified this enzyme (named sapb) and we cloned, seque ...200818397761
extracellular proteolytic activities expressed by bacillus pumilus isolated from endodontic and periodontal lesions.the purpose of the present study was to identify 12 bacillus isolates that had been obtained from root canals of teeth requiring endodontic therapy and from periodontal pockets in severe marginal periodontitis, and to determine whether these isolates exhibited extracellular proteolytic activity and, using in vitro assays, whether any such activity could degrade substrates that would be pathophysiologically relevant with regard to the production of endodontic and periodontal lesions. biochemical ...200818436599
a novel family of antimicrobial peptides from the skin of amolops loloensis.while conducting experiments to investigate antimicrobial peptides of amphibians living in the yunnan-sichuan region of southwest china, a new family of antimicrobial peptides was identified from skin secretions of the rufous-spotted torrent frog, amolops loloensis. members of the new peptide family named amolopins are composed of 18 amino acids with a unique sequence, for example, nilssivnginralsffg. by blast search, amolopins did no show similarity to any known peptides. among the tested micro ...200818312859
species-level identification of bacillus strains isolates from marine sediments by conventional biochemical, 16s rrna gene sequencing and inter-trna gene sequence lengths analysis.the aim of this study was to compare the ability of commonly used conventional biochemical tests, sequencing analysis of 16s rrna genes and tdna-intergenic spacer length polymorphism (tdna-pcr) to identify species of the genus bacillus recovered from marine sediments. while biochemical tests were not sufficiently sensitive to distinguish between the 23 marine strains analyzed, partial 16s rrna gene sequences allowed a correct identification, clustering them into four species belonging to bacillu ...200817922298
characterization of an alkaline active-thiol forming extracellular serine keratinase by the newly isolated bacillus pumilus.the aim of the study was to optimize microbial degradation of keratinous waste and to characterize the alkaline active keratinase showing its biotechnological importance.200817922821
nutritional regulation of keratinolytic activity in bacillus pumilis.bacillus pumilis f3-4 utilized feather as a sole source of carbon, nitrogen and sulfur. supplementation of the feather medium with glucose or mgso(4) . 7h(2)o increased keratinolytic protease production (14.6-16.7 u/mg). the synthesis of keratinolytic protease was repressed by an exogenous nitrogen source. keratinolytic protease was produced in the absence of feather (9.4 u/mg). feather degradation resulted in sulfhydryl group formation (0.8-2.6 microm). b. pumilis f3-4 effectively degraded chic ...200817968508
purification and characterization of an extracellular alpha-l-arabinosidase from a novel isolate bacillus pumilus ara and its over-expression in escherichia coli.the alpha-l-arabinosidase, arab, was induced when bacillus pumilus ara was grown at 50 degrees c in a minimal medium containing xylan. a 56-kda protein with alpha-l-arabinosidase activity was purified from culture supernatant to gel electrophoretic homogeneity. the optimal activity was at ph 6.4 and 60 degrees c over a 10-min assay. the purified enzyme was stable over a ph range of 5.2-7.6 and had a 1-h half life at 70 degrees c. the enzyme released arabinose from oat spelt xylan. kinetic experi ...200818071642
effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation.spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ...200818083857
in vitro inhibitory activity of probiotic spore-forming bacilli against genotoxins.to investigate the ability of bacilli of various species (bacillus clausii, bacillus subtilis, bacillus lentus, bacillus pumilus. bacillus megaterium, bacillus firmus, bacillus sp.) and origins (probiotic and collection strains) to counteract the activity of some representative dna-reactive agents.200818194161
isolation and assessment of phytate-hydrolysing bacteria from the delmarva peninsula.the delaware-maryland-virginia (delmarva) peninsula, flanking one side of the chesapeake bay, is home to a substantial broiler chicken industry. as such, it produces a significant amount of manure that is typically composted and spread onto local croplands as a fertilizer. phytate (myo inositol hexakisphosphate), the major form of organic phosphorus in the manure, can be hydrolysed by microorganisms to produce orthophosphate. orthophosphate is a eutrophication agent which can lead to algal bloom ...200717991037
central venous catheter infection with bacillus pumilus in an immunocompetent child: a case report.bacillus organisms are common laboratory contaminants. the majority of bacillus bacteraemias are transient and not clinically significant. clinically significant infection due to bacillus species is rare and mostly due to bacillus cereus infections in immuno-compromised hosts.200717967173
endophytic bacteria in sunflower (helianthus annuus l.): isolation, characterization, and production of jasmonates and abscisic acid in culture medium.this study was designed to isolate and characterize endophytic bacteria from sunflower (helianthus annuus) grown under irrigation and water stress (drought) conditions, to analyze growth of isolated bacteria under drought condition, and to evaluate the ability of bacteria isolated from plants cultivated under drought to produce jasmonates (jas) and abscisic acid (aba). bacteria were isolated from soil samples collected when sunflower plants were at the end of the vegetative stage. a total of 29 ...200717657487
pumilicin 4, a novel bacteriocin with anti-mrsa and anti-vre activity produced by newly isolated bacteria bacillus pumilus strain wapb4.a total of 34 bacterial strains with anti-methicillin-resistant staphylococcus aureus (mrsa) activity were isolated from 69 soil and water samples collected from four areas of thailand. one strain, wapb4 identified as bacillus pumilus, showed remarkable antibacterial activity against mrsa, vancomycin-resistant enterococcus faecalis (vre), and several gram-positive test bacteria. bacteriocin produced by wapb4 was designated as pumilicin 4. it was heat stable up to 121 degrees c, 15 min and active ...200717700984
production of thermostable pectinase and xylanase for their potential application in bleaching of kraft pulp.a very high level of alkalophilic and thermostable pectinase and xylanase has been produced from newly isolated strains of bacillus subtilis and bacillus pumilus respectively. enzyme production for pectinase was carried out under ssf using combinations of cheap agricultural residues while xylanase was produced under submerged fermentation using wheat bran as substrate to minimize the cost of production of these enzymes among the various substrates tested, the highest yield of pectinase productio ...200717726619
paradoxical dna repair and peroxide resistance gene conservation in bacillus pumilus safr-032.bacillus spores are notoriously resistant to unfavorable conditions such as uv radiation, gamma-radiation, h2o2, desiccation, chemical disinfection, or starvation. bacillus pumilus safr-032 survives standard decontamination procedures of the jet propulsion lab spacecraft assembly facility, and both spores and vegetative cells of this strain exhibit elevated resistance to uv radiation and h2o2 compared to other bacillus species.200717895969
bacillus pumilus sg2 isolated from saline conditions produces and secretes two chitinases.isolation and characterization of chitinases from a halotolerant bacillus pumilus.200717897213
microbiological safety and clinical efficacy of radiation sterilized amniotic membranes for treatment of second-degree burns.amniotic membranes collected from the placentae of screened donors were processed and sterilized by gamma irradiation at 25 kgy. the sterility assurance level (sal) of gamma irradiated amniotic membranes and clinical efficacy in second-degree burn wound healing were evaluated. processed air-dried amniotic tissue from 159 batches of processing was checked for the bioburden level before sterilization. about 39% of the tissues had bioburden in the range of 10(1)-10(2)/100 cm(2) and 54.8% in the ran ...200717182190
screening and mutagenesis of a novel bacillus pumilus strain producing alkaline protease for dehairing.to characterize and optimize a novel bacillus pumilus strain isolated from biological waste which produces protease with excellent dehairing effect. this newly isolated strain could be utilized in the industrial leather dehairing process.200717209806
cutaneous infection due to bacillus pumilus: report of 3 cases.human infection due to bacillus pumilus is exceptional. we report 3 cases of cutaneous infection caused by b. pumilus that occurred in 3 shepherds, 2 of whom were members of the same family. the lesions appeared to have a morphology similar to that of cutaneous anthrax lesions. two patients were cured after treatment with amoxicillin-clavulanate, and the third patient was cured after prolonged treatment with ciprofloxacin. to our knowledge, primary cutaneous infection due to b. pumilus has not b ...200717243047
food poisoning associated with pumilacidin-producing bacillus pumilus in rice.food poisoning caused by other bacillus species than b. cereus has been described, but the toxins involved have rarely been isolated. endospores will survive heat treatment and will germinate and multiply in cooked foods producing toxins under appropriate conditions. we describe a small food poisoning outbreak where three people became ill after a dinner in a chinese restaurant. acute symptoms including dizziness, headache, chills and back pain developed during the meal, and a few hours later th ...200717275116
antimicrobial activity of bacillus subtilis and bacillus pumilus during the fermentation of african locust bean (parkia biglobosa) for soumbala production.to examine predominant isolates of bacillus subtilis and b. pumilus isolated from soumbala for their antimicrobial activity against indicator microorganisms as micrococcus luteus, staphyloccocus aureus, bacillus cereus, enterococus facium, listeria monocytogenes, escherichia coli, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica, aspergillus ochraceus and penicillium roqueforti.200717381739
comparison of four antibiotics with indigenous marine bacillus spp. in controlling pathogenic bacteria from shrimp and artemia.use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. this has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. this study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. ten pathogenic bacterial species isolated from shrimp, penaeus monodon, and artemia cysts were tested for susceptibility to indigenous marine bacillus sub ...200717584435
probiotics reduce infectious complications after pancreaticoduodenectomy.postoperative morbidity is a significant problem associated with pancreaticoduodenectomy. the clinical value of probiotics in surgical patients remains unclear. this study investigated the effect of probiotics on surgical outcome after pancreaticoduodenectomy.200717591036
toxinogenic bacillus pumilus and bacillus licheniformis from mastitic milk.to elucidate the occurrence of heat-stable toxin-producing strains among mastitic bacillus isolates, 100 milk samples of mastitic cows from different parts of finland were screened. bacillus was identified as the major organism in 23 samples. toxinogenic bacillus isolates identified by sperm cell motility inhibition assay were isolated from six samples. four isolates belonged to the species bacillus pumilus and two to bacillus licheniformis. the toxic substances were heat-stable and soluble to m ...200717611049
cytotoxicity in bacillus mojavensis is abolished following loss of surfactin synthesis: implications for assessment of toxicity and food poisoning potential.bacillus subtilis and the closely related species bacillus pumilus and bacillus licheniformis have periodically been suggested to play a role in the aetiology of food poisoning despite the fact that the organisms do not possess the genes associated with enteropathogenicity in bacillus cereus. we show here that bacillus mojavensis, an organism closely related to b. subtilis, is able to produce toxic components which identify as a complex of three different surfactin analogues. these cyclic lipope ...200717467096
[cloning, characterization and application of the promoter of alkaline protease gene in bacillus pumilus].a 797 bp promoter fragment of alkaline protease gene was cloned from the bacillus pumilus genome by employing tail-pcr strategy. the sequence analysis of this promoter fragment showed that the sequence accounting for gene expression was approximately 390bp. deletion analysis of the fragment defined the minimal required sequence of promoter for initiating transcription lies on a 160 bp region upstream of the start codon. the alkaline protease gene wapq3 containing the cloned promoter fragment was ...200717646155
small rolling circle plasmids in bacillus subtilis and related species: organization, distribution, and their possible role in host physiology.bacillus subtilis and related species (bacillus licheniformis, bacillus pumilus, bacillus amyloliquefaciens, and bacillus mojavensis) represent a group of bacteria largely studied and widely employed by industry. small rolling circle replicating plasmids of this group of bacteria have been intensively studied as they represent a convenient model for genetic research and for the construction of molecular tools for the genetic modification of their hosts. through the computational analysis of the ...200717064773
immobilization of xylanase from bacillus pumilus strain mk001 and its application in production of xylo-oligosaccharides.xylanase from bacillus pumilus strain mk001 was immobilized on different matrices following varied immobilization methods. entrapment using gelatin (ge) (40.0%), physical adsorption on chitin (ch) (35.0%), ionic binding with q-sepharose (q-s) (45.0%), and covalent binding with hp-20 beads (42.0%) showed the maximum xylanase immobilization efficiency. the optimum ph of immobilized xylanase shifted up to 1.0 unit (ph 7.0) as compared to free enzyme (ph 6.0). the immobilized xylanase exhibited high ...200718025574
[conditions of the biosynthesis of an extracellular subtilisin-like proteinase by bacillus pumilus kmm 62].the influence of the cultivation conditions on bacillus pumilus kmm 62 growth and effectiveness of the production of a subtilisin-like serine proteinase were investigated. enzyme accumulation in the culture fluid reached the maximum value after 32 and 46-48 h of growth; it depends on the composition of the nutrient medium. the ratio of the concentrations of two main components of the medium, peptone and inorganic phosphate, which was optimal for enzyme biosynthesis was determined by multifactor ...200717633406
characterization of antimicrobial compounds from a common fern, pteris biaurita.methanol extract was prepared from the fronds of pteris biaurita and partial purification was done by solvent partitioning with diethyl ether and ethyl acetate, followed by hydrolysis and further partitioning with ethyl acetate. the three fractions, thus obtained were bioassayed separately against five test fungi--curvularia lunata, fomes lamaoensis, poria hypobrumea, fuasrium oxysporum and a bacterium--bacillus pumilus, by spore germination, radial growth and agar cup techniques. results reveal ...200717373375
use of protoplasts from paired heterogenic bacterial species to detect tin contaminants: prospects for biosensor development.two different bacteria gave different respiratory responses to the test analytes, tributyl tin (tbt) and cadmium as expressed by positive sigmoid responses by halomonas sp. (slope, +1.71 [tbt]; +1.76 [cd]) and negative sigmoid responses by bacillus pumilis (slope, -1.06 [tbt]; -0.59 [cd]). the ec50 values determined from hill plots for the response of halomonas sp. to the tbt and cd were 1 and 8.5 mm, respectively, which were lower by a factor of 10 than the corresponding values for b. pumilis. ...200716815699
development of a bacteriophage model system to investigate virus inactivation methods used in the treatment of bone allografts.bone allografts are commonly used in a variety of surgical procedures, to reconstruct lost bone stock and to provide mechanical support during the healing process. due to concerns regarding the possibility of disease transmission from donor to recipient, and of contamination of grafts during retrieval and processing procedures, it is common practice to sterilise bone allografts prior to issue for clinical use. it is vital that the sterilisation processes applied to allografts are validated to de ...200717061148
bismuth-norfloxacin complex: synthesis, physicochemical and antimicrobial evaluation.norfloxacin is a fluoroquinolone antibacterial agent which is active against various gram-positive as well as gram-negative microorganisms. presence of metal ions considerably alters the activity of fluoroquinolones against potentially susceptible bacteria. as bismuth is known to possess a good antibacterial activity, bismuth complex of norfloxacin was prepared by reacting bismuth citrate with aqueous solution of norfloxacin. the structure of the bismuth-norfloxacin complex (bnc) was confirmed b ...200717174046
clostridium butyricum to-a culture supernatant downregulates tlr4 in human colonic epithelial cells.the present study was performed to examine whether probiotics affect toll-like receptor 4 (tlr4) expression in human colonic epithelial cells. culture supernatants or heat-killed bacteria of bacillus mesentericus to-a, clostridium butyricum to-a, and streptococcus faecalis t-110 were applied to human colonic epithelial cells. treatment with c. butyricum to-a culture supernatant significantly reduced tlr4 mrna level (x0.16), even in the presence of interferon-gamma (ifn-gamma; x0.21) as compared ...200717404865
degradation of bisphenol a by bacillus pumilus isolated from kimchi, a traditionally fermented food.novel bisphenol a (bpa)-degrading bacterial strains, designated as bp-2ck, bp-21dk, and bp-22dk, were isolated from kimchi, a traditionally fermented food. these isolates were identified as bacillus pumilus and efficiently degraded bpa in a medium supplemented with nutrients such as peptone, beef extract, and yeast extract. strains bp-2ck, bp-21dk, and bp-22dk successfully degraded 25, 25, and 50 ppm of bpa, respectively, and all strains exhibited bpa-degrading activity in the presence of 10% na ...200717416976
a colorimetric assay for the determination of acetyl xylan esterase or cephalosporin c acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin c, or acetylated xylan as substrate.a bromothymol blue-based colorimetric assay has been devised to screen for acetyl xylan esterase or cephalosporin c (cpc) deacetylase activities using 7-amino cephalosporanic acid (7-aca), cpc, or acetylated xylan as substrate. these enzymes are not screened with their natural substrates because of the tedious procedures available previously. acetyl xylan esterase from bacillus pumilus cect 5072 was cloned, expressed in escherichia coli rosetta (de3), and characterized using this assay. similar ...200717651681
[molecular docking of bacillus pumilus xylanase and xylan substrate using computer modeling].bacillus pumilus xylanase was cloned and sequenced. based on the tertiary structure that originated from homology modeling, the potential active pocket was searched and ligand-protein docking was performed using relative softwares. the information extracted from the molecular docking is analyzed; several amino acid residues might play a vital role in the xylanase catalytic reaction are obtained to instruct the further modification of xylanase directed-evolution.200717822050
a new organic solvent tolerant protease from bacillus pumilus 115b.five out of the nine benzene-toulene-ethylbenzene-xylene (btex) tolerant bacteria that demonstrated high protease activity on skim milk agar were isolated. among them, isolate 115b identified as bacillus pumilus exhibited the highest protease production. the protease produced was stable in 25% (v/v) benzene and toluene and it was activated 1.7 and 2.5- fold by n-dodecane and n-tetradecane, respectively. the gene encoding the organic solvent tolerant protease was cloned and its nucleotide sequenc ...200717492323
[the effect of spo0a and abrb proteins on expression of the genes of guanyl-specific ribonucleases from bacillus intermedius and bacillus pumilus in bacillus subtilis recombinant strains].guanyl-specific ribonucleases from bacillus intermedius and bacillus pumilus are actively secreted under phosphate starvation by recombinant strains of bacillus subtilis with native regulatory systems and by strains defective in some proteins of the spo0a phosphorylation pathway. the level of expression of ribonuclease genes has been shown to increase approximately sixfold in recombinant strains with mutation in the spo0a gene and threefold in the spo0a/abrb mutants, as compared with native stra ...200718069324
isolation of 8'-phosphate ester derivatives of amicoumacins: structure-activity relationship of hydroxy amino acid moiety.two new compounds, 8'-phospho derivatives of amicoumacins a and b, were isolated from the culture broth of a strain of bacillus pumilus together with amicoumacins a and b. their structures were elucidated on the basis of spectroscopic methods and alkaline phosphatase treatments. comparison of the antibacterial activities against methicillin-resistant staphylococcus aureus (mrsa) of these compounds suggested that c-8' hydroxyl and c-12' amide group of amicoumacin a played a critical role for anti ...200718277000
ethanol/water pulps from sugar cane straw and their biobleaching with xylanase from bacillus pumilus.the influence of independent variables (temperature and time) on the cooking of sugar cane straw with ethanol/water mixtures was studied to determine operating conditions that obtain pulp with high cellulose contents and a low lignin content. an experimental 2(2) design was applied for temperatures of 185 and 215 degrees c, and time of 1 and 2.5 h with the ethanol/water mixture concentration and constant straw-to-solvent ratio. the system was scaled-up at 200 degrees c cooking temperature for 2 ...200718478412
production of cellulase-free endoxylanase from novel alkalophilic thermotolerent bacillus pumilus by solid-state fermentation and its application in wastepaper recycling.the present study aimed at optimization of culture condition for the enhanced production of extra cellular thermostable cellulase-free xylanase from bacillus pumilus by solid-state fermentation. batch studies were carried out to evaluate various agro-industrial residues such as rice bran, rice husk, rice straw, sawdust, coconut pith, sugarcane bagasse and wheat bran for enzyme production by the bacterial culture. the endoxylanase production was highest on wheat bran media (5582 u/gds), which was ...200716844369
biotransformation of isoeugenol to vanillin by a newly isolated bacillus pumilus strain: identification of major metabolites.a bacterial strain s-1 capable of transforming isoeugenol to vanillin was isolated. the strain was identified as bacillus pumilus based on biochemical tests, cellular fatty acid composition, riboprint pattern and 16s rrna gene sequence analyses. in the biotransformation of isoeugenol, vanillin was the main product. with the growing culture of b. pumilus s-1, 10g l(-1) isoeugenol was converted to 3.75g l(-1) vanillin in 150 h, with a molar yield of 40.5% that is the highest up to now. dehydrodiis ...200717583367
speciation of bacillus spp. in honey produced in northern ireland by employment of 16s rdna pcr and automated dna sequencing techniques.phenotypic speciation of foodborne bacillus spp. remains problematic in terms of obtaining a reliable identification. in this study, we wished to identify several bacterial isolates from honey produced in northern ireland, and which belonged to the genus bacillus, through employment of a molecular identification scheme based on pcr amplification of universal regions of the 16s rrna operon in combination with direct automated sequencing of the resulting amplicons. seven samples of honey and relat ...200727517837
use of bacterial extracts to enhance amino acid breakdown in dry fermented sausages.the effect of the intracellular cell-free extracts (icfes) of two bacterial strains (lactobacillus sakei go and bacillus pumilus) on the amino acid catabolism and the sensory properties of dry fermented sausages, was investigated. extracts were added to sausages alone or in combination with a protease, papain. amino acid breakdown was monitored by the changes in free amino acids, ammonia and amine content during the ripening process. a 15% decrease in the content of free amino acids was observed ...200622061560
bacillus aerius sp. nov., bacillus aerophilus sp. nov., bacillus stratosphericus sp. nov. and bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes.four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. the four strains, 24k(t), 28k(t), 41kf2a(t) and 41kf2b(t), were identified as members of the genus bacillus. phylogenetic analysis based on 16s rrna gene sequences indicated that three of the strains, 24k(t), 28k(t) and 41kf2a(t), are very similar to one another (>98 % sequence similarity) and show a similarity of 98-99 % with bacillus licheniformis and 98 % with bacillus ...200616825614
sterilizing bacillus pumilus spores using supercritical carbon dioxide.supercritical carbon dioxide (sc co(2)) has been evaluated as a new sterilization technology. results are presented on killing of b. pumilus spores using sc co(2) containing trace levels of additives. complete killing was achieved with 200 part per million (ppm) hydrogen peroxide in sc co(2) at 60 degrees c, 27.5 mpa. addition of water to sc co(2) resulted in greater than three-log killing, but this is insufficient to claim sterilization. neither ethanol nor isopropanol when added to sc co(2) af ...200616516991
characterization of bacterial growth and the influence of antibiotics by means of uv resonance raman spectroscopy.in this work we monitor the bacterial growth of a bacillus pumilus batch culture by means of uv resonance raman spectroscopy. excitation with a wavelength of 244 nm especially enhances the raman scattering of the aromatic amino acids and the nucleic acid bases and therefore is a good method to track the metabolic changes that occur during bacterial growth. furthermore, a drug from the fluoroquinolone group is added to the bacterial suspension at the beginning of the exponential growth phase. wit ...200616421857
[the role of the promoter structure in the efficiency of the expression of guanylspecific ribonucleases from bacillus intermedius and bacillus pumilus].guanylspecific ribonucleases from b. intermedius (binase) and b.pumilus (rnase bpu) are structural and functional homologues, and their biosynthesis is subjected to the same laws. at the same time, there are essential differences in the expression efficiency of binase and rnase bpu genes. this was first suggested to be due to differences in nucleotide sequences of promoters of the genes. therefore, we constructed plasmids changing each different nucleotide in binase promoter for corresponding on ...200617094653
[construction of an inducible and efficient expression-secretion shuttle vector of b. subtilis].a new shuttle vector psb which could replicate in both escherichia coli and bacillus subtilis was constructed by fusing the e. coli plasmid psp72 with the b. subtilis plasmid pub18. after the sacb promoter and signal peptide sequence of b. subtilis was inserted in the multiple cloning sites (mcs) of psb, the recombinant plasmid was designated as psbp. the alpha-amylase gene terminator of bacillus licheniformis was inserted at the other end of the mcs, resulting in expression-secretion plasmid ps ...200617172015
bacillus spp. among hospitalized patients with haematological malignancies: clinical features, epidemics and outcomes.between april 2000 and may 2005, 350 bacteraemic episodes occurred among patients treated in our haematology unit. two hundred and twenty-eight of these episodes were caused by gram-positive pathogens, most commonly coagulase-negative staphylococci and staphylococcus aureus. one hundred and twenty-two episodes were due to gram-negative pathogens, with a predominance of escherichia coli, acinetobacter baumannii and pseudomonas aeruginosa. bacillus bacteraemias constituted 12 of these episodes occ ...200616891037
bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces.thirteen strains of a novel spore-forming, gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces (mars odyssey orbiter) and assembly-facility surfaces at the jet propulsion laboratory in california and the kennedy space center in florida. phylogenetic analysis of 16s rrna gene sequences has placed these novel isolates within the genus bacillus, the greatest sequence similarity (99.9 %) being found with bacillus pumilus. however, these isolates share a mere 91.2 ...200616902000
identification and characterization of a gamma-glutamyl transpeptidase from a thermo-alcalophile strain of bacillus pumilus.a gamma-glutamyltranspeptidase (ggt, e.c. 2.3.2.2) was isolated from a strain (a8) originating from lake bogoria (kenya) and homologous with bacillus pumilus. this ggt shows an optimal activity at ph 8.9 and 62 degrees c. the enzyme is thermostable up to 43 degrees c. the best reagent among the potential inhibitors was shown to be don, which is an inhibitor highly specific for ggts. gly-gly-ala, gly-gly-gly and gly-gly were identified as the best acceptors for the transpeptidation reactions cata ...200617043758
phenotypic characterization of food waste degrading bacillus strains isolated from aerobic bioreactors.a phenotypic characterization of seventeen bacillus strains isolated from aerobic thermophilic bioreactors of a food waste processing company was carried out, using fatty acid and enzymatic activity profiles. it was observed that each species possessed a typical fatty acid and enzymatic production profile. bacillus licheniformis strains exhibited the most significant enzyme production. numerical analyses (principal component and hierarchical cluster analyses) revealed that bacillus licheniformis ...200616463317
identification of nif genes in n2-fixing bacterial strains isolated from rice fields along the yangtze river plain.the aim of this research was to identify nifh and nifhdkye ' genes in twenty strains of n2-fixing heterotrophic bacteria isolated from rice fields in the yangtze river plain. southern hybridization of the total dna from each strain was performed with the klebsiella pneumoniae nifhdkye ' gene probe (6.2 kb eco ri fragment from psa30) and the azospirillum brasilense nifh gene probe (0.6 kb eco ri-hin diii fragment from phu8). we found that eco ri fragments of total dna from aeromonas hydrophila hy ...200616463319
purification and molecular characterization of subtilisin-like alkaline protease bpp-a from bacillus pumilus strain ms-1.the present study was conducted by screening zein-degrading bacteria in an attempt to obtain zein-degrading protease.200616478511
selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities.fifteen bacterial strains isolated from solid waste oil samples were selected due to their capacity of growing in the presence of hydrocarbons. the isolates were identified by pcr of the 16s rdna gene using fd1 and rd1 primers. the majority of the strains belonged to genera bacillus, bacillus pumilus (eight strains) and bacillus subtilis (two strains). besides, three strains were identified as micrococcus luteus, one as alcaligenes faecalis and one strain as enterobacter sp. growth of the above- ...200616564960
xylose utilization and short-chain fatty acid production by selected components of the intestinal microflora of a rodent pollinator (aethomys namaquensis).namaqua rock mice (aethomys namaquensis) consume nectar xylose when visiting protea flowers. whole-animal metabolism studies suggest that the gastrointestinal microflora plays an important role in xylose metabolism in a. namaquensis. we collected caecal contents under anaerobic conditions, cultured caecal microflora both aerobically and anaerobically, and assessed caecal microbial xylose utilization using a (14)c-xylose incubation assay. all four mice sampled hosted culturable caecal micro-organ ...200616676189
search for antibacterial and antifungal agents from selected indian medicinal plants.a series of 61 indian medicinal plants belonging to 33 different families used in various infectious disorders, were screened for their antimicrobial properties. screening was carried out at 1000 and 500 microg/ml concentrations by agar dilution method against bacillus cereus var mycoides, bacillus pumilus, bacillus subtilis, bordetella bronchiseptica, micrococcus luteus, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, streptoco ...200616678369
production of indole-3-acetic acid in the plant-beneficial strain pseudomonas chlororaphis o6 is negatively regulated by the global sensor kinase gacs.certain plant growth-promoting bacteria, such as pseudomonas fluorescens 89b61 and bacillus pumilus se34, secreted high levels of indole-3-acetic acid (iaa) in tryptophan-amended medium in stationary phase as determined by chromogenic analysis and high-performance liquid chromatography. two other growth-promoting strains, p. chlororaphis o6 and serratia marcescens 90-166, did not produce these high levels of iaa. however, when the gacs mutant of p. chlororaphis o6 was grown in tryptophan-supplem ...200616732458
functional evaluation of a novel constitutive promoter f1 of bacillus pumilus, as a rice epiphytic strain, and construction of an efficient expression and secretion system under the control of f1.to establish a constitutive, high-efficiency expression and secretion system for bacillus pumilus, the function of a promoter and the abilities of three signal peptides in b. pumilus dx01 were tested. f1, cloned from the rice epiphyte b. pumilus strain dx01, had strong transcription activity and was a vegetative-phase constitutive promoter. the signal sequences of bacillus subtilis levansucrase (sacb) and subtilisin, as well as b. pumilus dx01 rnase signal sequence could drive the secretion of e ...200616799766
a marked enhancement in the production of a highly alkaline and thermostable pectinase by bacillus pumilus dcsr1 in submerged fermentation by using statistical methods.the production of a highly alkaline and thermostable pectinase of bacillus pumilus was optimized in submerged fermentation using plackett-burman design and response surface methodology. three fermentation variables (c:n ratio, k(2)hpo(4), and ph), which were identified to significantly affect pectinase production by plackett-burman design were further optimized using response surface methodology of central composite design (ccd). an over all 34- and 41-fold increase in enzyme production was achi ...200615936940
purification and characterization of a new bioscouring pectate lyase from bacillus pumilus bk2.an alkalophilic bacterium was isolated based on the potential of extra-cellular enzymes for bioscouring. the bacterium was identified as a new strain of bacillus pumilus bk2 producing an extra-cellular endo-pectate lyase pl (ec 4.2.2.2). pl was purified to homogeneity in three steps and has a molecular mass of 37.3+/-4.8 kda as determined by sds-page and an isoelectric point of ph 8.5. peptide mass mapping by nano-lc-ms of pl revealed 15% homology with a pectate lyase from bacillus sp. the pecta ...200616168510
a case of persistent bacillus pumilis bacteremia associated with cholangitis. 200615992931
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