Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| recurrence of deep surgical site infection in cerebral palsy after spinal fusion is rare. | retrospective review of prospective registry. | 2017 | 28449964 |
| synthesis and bioactivity evaluation of novel 2-salicyloylbenzofurans as antibacterial agents. | in order to discover new antibacterial agents, series of 2-salicyloylbenzofuran derivatives were designed, synthesized and evaluated for their antibacterial activities against three gram-(+) strains (methicillin-sensitive staphylococcus aureus (mssa) atcc 29213, methicillin-resistant staphylococcus aureus (mrsa) atcc 43300, and streptococcus faecalis (s. faecalis) atcc 29212) and one gram-(-) strain (escherichia coli (e. coli) atcc 25922). the 2-salicyloylbenzofuran heterocycles were generated b ... | 2017 | 28441350 |
| substance p enhances lactic acid and tyramine production in enterococcus faecalis v583 and promotes its cytotoxic effect on intestinal caco-2/tc7 cells. | enterococcus faecalis, generally considered as a saprophytic bowel commensal, has recently emerged as an important nosocomial pathogen causing severe urinary tract infections, surgical wound infections, bacteremia, and bacterial endocarditis. this bacterium is capable of forming biofilms on various surfaces and its high level of antibiotic resistance contributes to its pathogenicity. the aim of this study was to evaluate the effect on e. faecalis, of substance p (sp), an antimicrobial peptide th ... | 2017 | 28439299 |
| comparison of linezolid and vancomycin lock solutions with and without heparin against biofilm-producing bacteria. | the activity of linezolid and vancomycin lock solutions against biofilm-producing strains of staphylococcus aureus, s. epidermidis, and enterococcus faecalis was studied. | 2017 | 28438824 |
| comparative evaluation of antimicrobial efficacy of an alternative natural agent for disinfection of toothbrushes. | the aim of this study was to assess the antimicrobial potency of aqueous extract of psidium guajava leaves in two different concentrations as a toothbrush disinfectant against three oral bacterial species. | 2017 | 28435376 |
| dual-targeting small-molecule inhibitors of the staphylococcus aureus fmn riboswitch disrupt riboflavin homeostasis in an infectious setting. | riboswitches are bacterial-specific, broadly conserved, non-coding rna structural elements that control gene expression of numerous metabolic pathways and transport functions essential for cell growth. as such, riboswitch inhibitors represent a new class of potential antibacterial agents. recently, we identified ribocil-c, a highly selective inhibitor of the flavin mononucleotide (fmn) riboswitch that controls expression of de novo riboflavin (rf, vitamin b2) biosynthesis in escherichia coli. he ... | 2017 | 28434876 |
| disinfection of water inoculated with enterococcus faecalis using solar/fe(iii)edds-h2o2 or s2o8(2-) process. | in this study, the activation of h2o2 and persulfate ions induced by solar photolysis of fe(iii)edds complex were investigated in water disinfection, applying solar aops processes. the use of fe(iii)edds complex maintains iron in soluble form until slightly basic ph and so the photolysis is efficient in a large range of ph compatible with natural waters. moreover, for the first time, the impact of photogenerated hydroxyl and sulfate radicals on the inactivation of enterococcus faecalis in water ... | 2017 | 28433695 |
| diversity of integrative and conjugative elements of streptococcus salivarius and their intra- and interspecies transfer. | integrative and conjugative elements (ices) are widespread chromosomal mobile genetic elements which can transfer autonomously by conjugation in bacteria. thirteen ices with a conjugation module closely related to that of icest3 of streptococcus thermophilus were characterized in streptococcus salivarius by whole-genome sequencing. sequence comparison highlighted ice evolution by shuffling of 3 different integration/excision modules (for integration in the 3' end of the fda, rpsi, or rpmg gene) ... | 2017 | 28432093 |
| [effects of application of vancomycin in the early stage of patients with extremely severe burn]. | objective: to evaluate the effects of application of vancomycin in the early stage of patients with extremely severe burn, in order to provide reference to drug for anti-infection treatment in the early stage of patients with extremely severe burn. methods: data of 15 patients of kunshan explosion on august 2nd, 2014, admitted to the department of intensive care in our hospital were retrospectively analyzed. the clinical efficacy of continuously intravenous dripping of vancomycin (combined with ... | 2017 | 28427133 |
| correction: chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against enterococcus faecalis biofilms. | [this corrects the article doi: 10.1371/journal.pone.0174888.]. | 2017 | 28426772 |
| comprehensive analysis of phospholipids and glycolipids in the opportunistic pathogen enterococcus faecalis. | enterococcus faecalis is a gram-positive, opportunistic, pathogenic bacterium that causes a significant number of antibiotic-resistant infections in hospitalized patients. the development of antibiotic resistance in hospital-associated pathogens is a formidable public health threat. in e. faecalis and other gram-positive pathogens, correlations exist between lipid composition and antibiotic resistance. resistance to the last-resort antibiotic daptomycin is accompanied by a decrease in phosphatid ... | 2017 | 28423018 |
| split tasks of asymmetric nucleotide-binding sites in the heterodimeric abc exporter efrcd. | many heterodimeric atp-binding cassette (abc) exporters evolved asymmetric atp-binding sites containing a degenerate site incapable of atp hydrolysis due to noncanonical substitutions in conserved sequence motifs. recent studies revealed that nucleotide binding to the degenerate site stabilizes contacts between the nucleotide-binding domains (nbds) of the inward-facing transporter and regulates atp hydrolysis at the consensus site via allosteric coupling mediated by the d-loops. however, it is u ... | 2017 | 28417533 |
| effect of instrumentation techniques and preparation taper on apical extrusion of bacteria. | the aim of this in vitro study was to evaluate the effects of different root canal instrumentation techniques and preparation tapers on the amount of apically extruded bacteria. | 2017 | 28416303 |
| effect of bacterial biofilm on the osteogenic differentiation of stem cells of apical papilla. | although clinical success in regenerative endodontics is substantially high, histological success is limited to finding bone/cementum-like tissue instead of dentin within the canal space. the aims of this study were to investigate (1) the effect of bacterial biofilm on osteogenic gene expression in stem cells of the apical papilla (scap) and (2) the effect of bacterial antigens on the functional differentiation of scap into a mineralizing phenotype. | 2017 | 28416302 |
| bacterial epidemiology and antimicrobial resistance profiles of urinary specimens of the elderly. | although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles. | 2017 | 28408060 |
| preventive effects of heat-killed enterococcus faecalis strain ec-12 on mouse intestinal tumor development. | establishing effective methods for preventing colorectal cancer by so-called "functional foods" is important because the global burden of colorectal cancer is increasing. enterococcus faecalis strain ec-12 (ec-12), which belongs to the family of lactic acid bacteria, has been shown to exert pleiotropic effects, such as anti-allergy and anti-infectious effects, on mammalian cells. in the present study, we aimed to evaluate the preventive effects of heat-killed ec-12 on intestinal carcinogenesis. ... | 2017 | 28406434 |
| peptidoglycan o-acetylation increases in response to vancomycin treatment in vancomycin-resistant enterococcus faecalis. | vancomycin resistance is conferred upon vancomycin-resistant enterococci (vre) through the replacement of peptidoglycan (pg) stem terminal d-ala-d-ala with d-ala-d-lac. the d-ala-d-lac incorporation can affect both the fitness and virulence of vre. here we comprehensively investigate the changes to pg composition in vancomycin-resistant enterococcus faecalis following the growth in presence of vancomycin using liquid chromatography-mass spectrometry. using high-resolution mass spectrometry, 104 ... | 2017 | 28406232 |
| rna and a cell wall component of enterococcus faecalis ic-1 are required for phagocytosis and interleukin 12 production by the mouse macrophage cell line j774.1. | enterococcus faecalis is a resident lactic acid bacterium in the human intestine. its immunostimulatory action was reported to be enhanced by heat sterilization. to investigate its beneficial actions, we evaluated the ability of 10 e. faecalis strains to induce interleukin-12 (il-12) production in a mouse macrophage cell line, j774.1 and found that the strain, e. faecalis ic-1, had a potent il-12-inducing ability. furthermore, we investigated the underlying mechanism by treating ic-1 cells with ... | 2017 | 28406067 |
| antibiotic resistant airborne bacteria and their multidrug resistance pattern at university teaching referral hospital in south ethiopia. | hospitals provide a reservoir of microorganisms, many of which are multi-resistant to antibiotics. emergence of multi-drug resistant strains in a hospital environment, particularly in developing countries is an increasing problem to infection treatment. this study aims at assessing antibiotic resistant airborne bacterial isolates. | 2017 | 28403894 |
| strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic lactobacillus spp. | urinary tract infections (utis), one of most common infections worldwide, face high recurrence rates and increasing antimicrobial resistance. probiotic bacteria, especially of the genus lactobacillus, are considered a promising preventive and/or treatment therapy against utis. in order to elucidate the mechanisms involved in these beneficial effects, we studied the impact of different lactobacillus strains (lactobacillus salivarius ucm572, l. plantarum clc17 and l. acidophilus 01) in the adheren ... | 2017 | 28402532 |
| wound infection secondary to snakebite. | snakebites can produce severe local and systemic septic complications as well as being associated with significant overall morbidity and even mortality. | 2017 | 28395682 |
| stochasticity among antibiotic-resistance profiles of common burn-related pathogens over a six-year period. | one of the most significant contributors to morbidity and death in patients with burns is infection, which accounts for 30%-75% of post-burn fatalities. because of concerns for the development of antibiotic resistance in burn-related pathogens, the aims of this study were to identify antibiotic resistance trends for the four most common burn-related pathogens over a six-year period. | 2017 | 28394746 |
| examination of enterococcus faecalis toxin-antitoxin system toxin fst function utilizing a pheromone-inducible expression vector with tight repression and broad dynamic range. | tools for regulated gene expression in enterococcus faecalis are extremely limited. in this report, we describe the construction of an expression vector for e. faecalis, designated pcie, utilizing the pq pheromone-responsive promoter of plasmid pcf10. we demonstrate that this promoter is tightly repressed, responds to nanogram quantities of the peptide pheromone, and has a large dynamic range. to demonstrate its utility, the promoter was used to control expression of the toxic peptides of two pa ... | 2017 | 28348028 |
| yeasts harbored by vespine wasps in the pacific northwest. | the ecological role of social wasps has been extensively studied, but little is known about symbiotic relationships of these wasps with microbes. recently, it was shown that vespid wasps in europe carry yeasts, predominantly saccharomyces cerevisiae, in their gastrointestinal (gi) tract. interestingly, this niche allowed for sexual recombination of yeasts to occur and the formation of novel hybrid species. our goals were 1) to survey the gi tract of eusocial wasps in the pacific northwest for th ... | 2017 | 28169397 |
| physicochemical characterization and antibacterial activity of rajata bhasma and silver nanoparticle. | bhasma, an ayurvedic metallo-mineral preparation, is claimed to be biologically produced nanoparticles. rajata (silver) is a noble metal known for its antimicrobial activity. rajata bhasma (rb) is expected to be composed of nanoparticles. with all these facts in place, this study was conducted to evaluate rb for the presence of silver nanoparticle (snp) and its antimicrobial effect. | 2017 | 28827959 |
| diversity of vaginal lactic acid bacterial microbiota in 15 algerian pregnant women with and without bacterial vaginosis by using culture independent method. | bacterial vaginosis (bv) is the most common lower genital tract disorder among women of reproductive age (pregnant and non-pregnant) and a better knowledge of lactobacillus species richness in healthy and infected vaginal microbiota is needed to efficiently design better probiotic products to promote the maintenance of normal flora which will help prevent bacterial vaginosis. | 2016 | 27790434 |
| synthesis and characterization of new chlorhexidine-containing nanoparticles for root canal disinfection. | root canal system disinfection is limited due to anatomical complexities. better delivery systems of antimicrobial agents are needed to ensure efficient bacteria eradication. the purpose of this study was to design chlorhexidine-containing nanoparticles that could steadily release the drug. the drug chlorhexidine was encapsulated in poly(ethylene glycol)-block-poly(l-lactide) (peg-b-pla) to synthesize bilayer nanoparticles. the encapsulation efficiency was determined through thermogravimetric an ... | 2016 | 28773576 |
| the effect of gelatinase production of enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants. | objectives: the aim of this study was to evaluate whether the gelatinase production ability of enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. materials and methods: standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (naocl), group 2: 2% chlorhexidine (chx), group 3: naocl + saline + chx, group 4: naocl + edta + naocl, group 5: qmix. after incubati ... | 2016 | 28642925 |
| microbiology of root canal infections. | inflammatory reaction in the periapical tissues is induced by microbial infection in the root canal system. the aim of root canal treatment is to preserve healthy periapical tissues or to provide healing of them in restorable teeth, which have sufficient periodontal support. the amount of microbial cells in the root canal system and their virulence as well as host responses influence on the degree of periapical inflammation and symptoms. microbial biofilm formation is typically seen on root cana ... | 2016 | 28826437 |
| integrating highly quantitative proteomics and genome-scale metabolic modeling to study ph adaptation in the human pathogen enterococcus faecalis. | genome-scale metabolic models represent the entirety of metabolic reactions of an organism based on the annotation of the respective genome. these models commonly allow all reactions to proceed concurrently, disregarding the fact that at no point all proteins will be present in a cell. the metabolic reaction space can be constrained to a more physiological state using experimentally obtained information on enzyme abundances. however, high-quality, genome-wide protein measurements have been chall ... | 2016 | 28725473 |
| in vitro antimicrobial activity screening of xylaria hypoxylon. | fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey. | 2016 | 28852719 |
| anti-inflammatory and regenerative effects of albanian propolis in experimental vital amputations. | this study evaluated the effects of albanian propolis on the inflamed pulpal tissue after pulpotomy in piglets. in five piglets, two teeth each were infected using special pathogenic microbial flora that was prepared in advance in order to cause inflammation of the pulp. pulpotomy was performed in the maxillary and mandibular central and lateral incisors. microbial flora pathogenesis prepared from the section of infected teeth, containing staphylococcus aureus, streptococcus viridans, enterococc ... | 2016 | 28509506 |
| [multivalvular infective endocarditis with enterococcus faecalis]. | infective endocarditis (ie) is a relatively rare disease that can take part of sepsis and may be a diagnostic and therapeutic challenge. polyvalvular location of el is rare. we present a patient of 60 years diagnosed with plurivalvular infective endocarditis by enterococcus faecalis with septic complications, visceral, dragged, hospitalized for a psychiatric pathology, who raised the problems of diagnosis and treatment, without being able to make assessments on the entrance gate. for help in dia ... | 2016 | 23077930 |
| phenolic content, antibacterial and antioxidant activities of erica herbacea l. | antibacterial and antioxidant activity, total phenolic and flavonoid concentrations of aqueous, ethanol and ethyl acetate extracts from the leaves and flowers of erica herbacea l. were studied. in vitro antibacterial activity of the extracts was determined by macrodilution method. minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) have been determined. testing was performed on 30 clinical isolates, including different strains of escherichia coli, enterococcus fae ... | 2016 | 24383325 |
| effect of a surfactant on the antimicrobial activity of sodium hypochlorite solutions. | the objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (naocl) associated with a surfactant. seventy single-rooted extracted human teeth were inoculated with enterococcus faecalis, and incubated for 21 days (37 °c). the groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% naocl; 5%, 2.5% and 1% hypoclean®, a solution containing a surfactant (cetrimide) associated with naocl. three microbiolo ... | 2016 | 25517777 |
| mechanical reduction of the intracanal enterococcus faecalis population by hyflex cm, k3xf, protaper next, and two manual instrument systems: an in vitro comparative study. | in the present study, the effectiveness of three rotary and two manual nickel titanium instrument systems on mechanical reduction of the intracanal enterococcus faecalis population was evaluated. | 2016 | 25424649 |
| killing of vre enterococcus faecalis by commensal strains: evidence for evolution and accumulation of mobile elements in the absence of competition. | enterococci are members of the gastrointestinal tract of humans and most animals that, over the past 3 decades, have emerged as leading causes of multidrug resistant hospital acquired infection (hai). in addition to their general hardiness, many traits have entered enterococcal lineages through horizontal gene transfer, which has led to the evolution of pathogenic hospital-associated lineages uniquely adapted for survival and proliferation in the antibiotic perturbed ecology of the gastrointesti ... | 2016 | 26939857 |
| daptomycin versus vancomycin in an enterococcus faecalis endophthalmitis rabbit model. | to compare bactericidal activities of daptomycin (dap) and vancomycin (van) in an experimental rabbit model of enterococcus faecalis endophthalmitis. | 2016 | 25658242 |
| antimicrobial activity of different disinfection methods against biofilms in root canals. | the aim of the present study was to evaluate the bactericidal effects of different disinfection methods against microbial biofilms grown in root canals. | 2016 | 25641829 |
| biological evaluation and molecular modelling study of thiosemicarbazide derivatives as bacterial type iia topoisomerases inhibitors. | in the present article, we describe the inhibitory potency of nine thiosemicarbazide derivatives against bacterial type iia topoisomerases, their antibacterial profile and molecular modelling evaluation. we found that one of the tested compounds, compound 7, significantly inhibits activity of staphylococcus aureus dna gyrase with an ic(50) below 15 μm. besides, this compound displays antibacterial activity on reference staphylococuss spp. and enterococcus faecalis strains as well as clinical s. ... | 2016 | 25792505 |
| purification and characterization of a bacteriocin from an oenological strain of leuconostoc mesenteroides subsp. cremoris. | malolactic fermentation (mlf), which improves organoleptic properties and biologic stability of some wines, may cause wine spoilage if uncontrolled. bacteriocins were reported as efficient preservatives to control mlf through their bactericidal effect on malolactic bacteria. leuconostoc mesenteroides subsp. cremoris w3 isolated from wine produces an inhibitory substance that is bactericidal against malolactic bacteria in model wine medium. treatment of the culture supernatant of strain w3 with p ... | 2016 | 25837975 |
| manual and rotary instrumentation ability to reduce enterococcus faecalis associated with photodynamic therapy in deciduous molars. | this aim of this study was to assess the ability of manual or rotary instrumentation associated with photodynamic therapy (pdt) to reduce enterococcus faecalis using three combinations of light/photosensitizers: toluidine blue o/laser, fuchsin/halogen light and fuchsin/led. twenty deciduous molars were selected and contaminated with enterococcus faecalis (mcfarland 0.5 scale). working length determination was performed by visual method. the teeth were randomly divided into two groups: g1 (n=10): ... | 2016 | 25590196 |
| time-dependent antibacterial effects of aloe vera and zataria multiflora plant essential oils compared to calcium hydroxide in teeth infected with enterococcus faecalis. | in the present in vitro study, we investigated the time-related antimicrobial efficacy of aloe vera and zataria multiflora (z. multiflora) plant essential oils compared to calcium hydroxide ([ca[oh]2 ) to eliminate enterococcus faecalis (e. faecalis) from root canals. | 2016 | 25187255 |
| assessing outcomes of adult oncology patients treated with linezolid versus daptomycin for bacteremia due to vancomycin-resistant enterococcus. | the incidence and severity of vancomycin-resistant enterococcus blood stream infections continue to rise and is a significant burden in the healthcare setting. literature thus far is minimal regarding treatment outcomes in patients with malignancy and vancomycin-resistant enterococcus bacteremia. appropriate antibiotic selection is vital to treatment success due to high rates of resistance, limited antimicrobials and mortality in this patient population. we conducted this study to determine whet ... | 2016 | 25326010 |
| different extracts of zingiber officinale decrease enterococcus faecalis infection in galleria mellonella. | dried, fresh and glycolic extracts of zingiber officinale were obtained to evaluate the action against g. mellonella survival assay against enterococcus faecalis infection. eighty larvae were divided into: 1) e. faecalis suspension (control); 2) e. faecalis + fresh extract of z. officinale (feo); 3) e. faecalis + dried extract of z. officinale (deo); 4) e. faecalis + glycolic extract of z. officinale (geo); 5) phosphate buffered saline (pbs). for control group, a 5 μl inoculum of standardized su ... | 2016 | 25831098 |
| efficacy of low concentrations of sodium hypochlorite and low-powered er,cr:ysgg laser activated irrigation against an enterococcus faecalis biofilm. | to establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without er,cr:ysgg laser activation on enterococcus faecalis biofilms in extracted teeth. | 2016 | 25772335 |
| the origin of endodontic enterococcus faecalis explored by comparison of virulence factor patterns and antibiotic resistance to that of isolates from stool samples, blood cultures and food. | to elucidate the origin of enterococcus faecalis isolated from secondary root canal infections and the possibility for a foodborne transmission by comparing them to strains recovered from food, blood and stool regarding putative virulence factors and antibiotic susceptibility profiles, where strains from common origin were hypothesized to harbour similar characteristics. | 2016 | 25950381 |
| transcription factor rex in regulation of pathophysiology in oral pathogens. | the nad(+) and nadh-sensing transcriptional regulator rex is widely conserved across gram-positive bacteria. rex monitors cellular redox poise and controls the expression of genes/operons involved in diverse pathways including alternative fermentation, oxidative stress responses, and biofilm formation. the oral cavity undergoes frequent and drastic fluctuations in nutrient availability, ph, temperature, oxygen tension, saliva, and shear forces. the oral streptococci are major colonizers of oral ... | 2016 | 26172563 |
| obturation techniques allow microbial leakage unless protected. | to evaluate the quality of the apical 5-mm seal produced by different filling techniques using a bacterial leakage model. | 2016 | 25996203 |
| antimicrobial 2-hydroxyisocaproic acid and chlorhexidine resist inactivation by dentine. | to compare the antibacterial activity of 2-hydroxyisocaproic acid (hica) with currently used root canal medicaments and to examine their interactions with potential inhibitors in nutrient-deficient and nutrient-rich conditions. | 2016 | 25944560 |
| l-lactic acid production from glycerol coupled with acetic acid metabolism by enterococcus faecalis without carbon loss. | glycerol is a by-product in the biodiesel production process and considered as one of the prospective carbon sources for microbial fermentation including lactic acid fermentation, which has received considerable interest due to its potential application. enterococcus faecalis isolated in our laboratory produced optically pure l-lactic acid from glycerol in the presence of acetic acid. gas chromatography-mass spectrometry analysis using [1, 2-(13)c2] acetic acid proved that the e. faecalis strain ... | 2016 | 26168904 |
| telavancin activity tested against gram-positive clinical isolates from european, russian and israeli hospitals (2011-2013) using a revised broth microdilution testing method: redefining the baseline activity of telavancin. | to reassess the activity of telavancin when tested against gram-positive clinical pathogens recovered from hospitalized patients in european and adjacent regions using a revised broth microdilution method. | 2016 | 26058844 |
| collagen-like peptide sequences inhibit bacterial invasion of root dentine. | to investigate the effects of peptides derived from the sequence of collagen to inhibit penetration of human or bovine dentine by species of streptococci and enterococci. | 2016 | 26011655 |
| pharmacokinetics of enrofloxacin and ceftiofur in plasma, interstitial fluid, and gastrointestinal tract of calves after subcutaneous injection, and bactericidal impacts on representative enteric bacteria. | this study's objectives were to determine intestinal antimicrobial concentrations in calves administered enrofloxacin or ceftiofur sodium subcutaneously, and their impact on representative enteric bacteria. ultrafiltration devices were implanted in the ileum and colon of 12 steers, which received either enrofloxacin or ceftiofur sodium. samples were collected over 48 h after drug administration for pharmacokinetic/pharmacodynamic analysis. enterococcus faecalis or salmonella enterica (5 × 10(5) ... | 2016 | 25989138 |
| confocal microscopy evaluation of the effect of irrigants on enterococcus faecalis biofilm: an in vitro study. | the purpose of this study was to evaluate in vitro the effectiveness of two endodontic irrigants and their association against enterococcus faecalis (e. faecalis) by confocal laser scanning microscope (clsm). twenty-four bovine incisors were inoculated in a monoculture of e. faecalis for 21 days. after this period, the teeth were divided into three test groups (n = 5) according to the chemical used. group 1: 2.5% sodium hypochlorite (naocl), group 2: 2% chlorhexidine gel (chx), group 3: 2.5% nao ... | 2016 | 26153228 |
| combined effects of photodynamic therapy and irrigants in disinfection of root canals. | in this study, the combined effects of photodynamic therapy and irrigants in eradicating common endodontic pathogens are evaluated. roots of 80 extracted single rooted teeth are divided into 2 groups (1) mechanical flushing; (2) antibacterial irrigation. after cleaning and shaping, they are inoculated with either (a) streptococcus mutans or (b) enterococcus faecalis and incubated. they are again subdivided and either only irrigated or irrigated and lased. dentin shavings are taken from root cana ... | 2016 | 26235897 |
| low level of resistance in enterococci isolated in four hospitals, marseille, france. | enterococci are gram-positive cocci responsible for various infections worldwide, and their prevalence of antibiotic resistance greatly varies worldwide. this study investigates the prevalence of resistance to antibiotics in enterococci from patients admitted in the four university hospitals of marseille between january 2013 and september 2014. two thousand nine hundred seventy-six patients-bacteria couples were identified (2,507 enterococcus faecalis and 469 enterococcus faecium) in the four un ... | 2016 | 26247097 |
| a comparison of the antibacterial activity of the two methods of photodynamic therapy (using diode laser 810 nm and led lamp 630 nm) against enterococcus faecalis in extracted human anterior teeth. | failure of endodontic treatment is usually due to an inadequate disinfection of the root canal system. enterococcus faecalis has been widely used as a valuable microbiological marker for in-vitro studies because of its ability to colonize in a biofilm like style in root canals, invading dentinal tubules and resistance to some endodontic treatments. the aim of this study was to investigate the antibacterial effects of two methods of photodynamic therapy using a light emitting diode lamp (led lamp ... | 2016 | 26241781 |
| investigation of mechanisms and molecular epidemiology of linezolid nonsusceptible enterococcus faecalis isolated from a teaching hospital in china. | the epidemiological and molecular characteristics of eight linezolid nonsusceptible enterococcus faecalis isolated from a teaching hospital in china (january to july 2014) were investigated. the target site modifications and cfr gene associated with linezolid resistance were not found. results of the epidemiological investigation indicated that linezolid resistance possibly occurred on several independent occasions and was often not related to linezolid administration. | 2016 | 26210761 |
| enterococcus faecalis endophthalmitis as a metastatic complication of hemodialysis vascular access-related sepsis: a case report and review of the literature. | catheter and/or arteriovenous (a-v) graft-related bacteremia is an important cause of morbidity and mortality among hemodialysis (hd) patients. endocarditis, septic arthritis, epidural abscess, septic embolism, and osteomyelitis are the most common complications of catheter and/or a-v graft-related bacteremia; however, endogenous endophthalmitis is rarely seen. to the best of our knowledge, enterococcus faecalis is the first case report in this population. we hereby report a case of endogenous e ... | 2016 | 26346615 |
| antimicrobial efficacy of photodynamic therapy, nd:yag laser and qmix solution against enterococcus faecalis biofilm. | lasers have been recommended in final root canal disinfection protocol, however, there is no clear evidence about their efficacy against bacteria in biofilms. the aim of the study was to evaluate and compare the disinfection effect of antimicrobial photodynamic therapy (apdt), nd:yag laser and qmix solution against enterococcus faecalis biofilm. | 2016 | 26232719 |
| how long can culturable bacteria and total dna persist in environmental waters? the role of sunlight and solid particles. | in this work, sunlight inactivation of two indicator bacteria in freshwater, with and without solid particles, was studied and the persistence of culturable cells and total dna was compared. environmental water was used to prepare two matrices, with and without solid particles, which were spiked with escherichia coli and enterococcus faecalis. these matrices were used to prepare microcosm bags that were placed in two containers: one exposed to sunlight and the other in the dark. during one month ... | 2016 | 26379262 |
| sealing ability of two root-end filling materials in a bacterial nutrient leakage model. | to compare in vitro the sealing ability of root-end fillings with mineral trioxide aggregate (mta) and endosequence bioceramic root repair material-fast set (bc-rrm) putty using a novel bacterial nutrient leakage model, which provides information on whether or not intracanal bacteria are receiving nutrients from serum via leakage channels. | 2016 | 26334201 |
| chitosan nanoparticles enhance the antibacterial activity of chlorhexidine in collagen membranes used for periapical guided tissue regeneration. | endodontic failure is mainly associated with the persistence of microbial infection in the root canal system and/or the periradicular area. microorganisms and their toxins located in the root canal system may trigger apical periodontitis and tissue destruction. tissue regeneration in periapical surgery by using membrane barriers has shown an improved healing and bone closure. however, bacterial membrane contamination is a main reason of failure. in this in vitro study, different brands of chlorh ... | 2016 | 26478419 |
| evaluation of tedizolid against staphylococcus aureus and enterococci with reduced susceptibility to vancomycin, daptomycin or linezolid. | tedizolid displays potent activity against gram-positive pathogens. in vitro studies against clinical isolates of staphylococcus aureus and enterococci demonstrated improved tedizolid activity over linezolid. however, this is not well-characterized against a large collection of resistant isolates, including vancomycin-intermediate s. aureus (visa), heterogeneous visa (hvisa), daptomycin-non-susceptible (dns) s. aureus, linezolid-resistant (lr) s. aureus and vre. therefore, our objective was to d ... | 2016 | 26476277 |
| surface protein esp enhances pro-inflammatory cytokine expression through nf-κb activation during enterococcal infection. | enterococcal surface protein (esp) is encoded on a pathogenicity island in enterococcus faecalis and e. faecium and is involved in biofilm formation and binding to epithelial cells. in this study, using esp-expressing e. faecalis mmh594 and its isogenic esp-deficient strain, as well as purified esp, we show that esp is sufficient for activation of nf-κb and the subsequent production of pro-inflammatory cytokines il-1β and tnf-α in macrophages in vitro. in a mouse peritonitis model, we also show ... | 2016 | 26503704 |
| high-level aminoglycoside resistance in enterococcus faecalis and enterococcus faecium causing invasive infection: twelve-year surveillance in the minami ibaraki area. | we examined prevalence of high-level aminoglycoside resistance (hlar) in enterococcus faecalis and enterococcus faecium causing invasive infection in the minami ibaraki area. ten strains of both species each, recovered from the blood or the cerebrospinal fluid between 2003 and 2014, were randomly selected every year. high-level resistance to gentamicin (hlr-gm) and streptomycin (hlr-sm) was detected in 34% (41 of 120 strains) and 18% (21) of e. faecalis and 9% (11) and 39% (48) of e. faecium, re ... | 2016 | 26492859 |
| prototype development of the intelligent hydrogel wound dressing and its efficacy in the detection of model pathogenic wound biofilms. | the early detection of wound infection in situ can dramatically improve patient care pathways and clinical outcomes. there is increasing evidence that within an infected wound the main bacterial mode of living is a biofilm: a confluent community of adherent bacteria encased in an extracellular polymeric matrix. here we have reported the development of a prototype wound dressing, which switches on a fluorescent color when in contact with pathogenic wound biofilms. the dressing is made of a hydrat ... | 2016 | 26492095 |
| relative contribution of biological variation and technical variables to zone diameter variations of disc diffusion susceptibility testing. | disc diffusion is still largely based on manual procedures. technical variations originate from inoculum preparation, variations in materials, individual operator plate streaking and reading accuracy. resulting measurement imprecision contributes to categorization errors. biological variation resembles the natural fluctuation of a measured parameter such as antibiotic susceptibility around a mean value. it is deemed to originate from factors such as genetic background or metabolic state. this st ... | 2016 | 26462987 |
| retrospective study for risk factors for febrile uti in spinal cord injury patients with routine concomitant intermittent catheterization in outpatient settings. | retrospective study. | 2016 | 26458969 |
| mature biofilms of enterococcus faecalis and enterococcus faecium are highly resistant to antibiotics. | enterococcus faecalis and enterococcus faecium are important nosocomial pathogens that form biofilms on implanted materials. we compare the antibiotic sensitivity of bacteria in new (established during 24 hours) and mature (established during 120 hours) enterococcal biofilms. mature biofilms contained more bacteria and were much more tolerant to antibiotics, including rifampicin-containing combinations, as judged by determination of minimal biofilm eradication concentrations and by time-kill exp ... | 2016 | 26458279 |
| covalent immobilization of enterococcus faecalis esawy dextransucrase and dextran synthesis. | enterococcus faecalis esawy dextransucrase was immobilized in fe(3+)-cross-linked alginate/carboxymethyl cellulose (ac) beads. the gel beads were modified with polyethylenimine (pei) followed by glutaraldehyde (ga) to form fe(3+) (acpg) beads. fe(3+) (acpg) was characterized using ftir and dsc techniques. ga activated beads showed new two peaks. the first was at 1,717 cm(-1) which refers to (co) group of a free aldehyde end of glutaraldehyde, and another peak was at 1,660 cm(-1) referring to (cn ... | 2016 | 26434519 |
| virulence of bacteria colonizing vascular bundles in ischemic lower limbs. | we documented previously the presence of bacterial flora in vascular bundles, lymphatics, and lymph nodes of ischemic lower limbs amputated because of multifocal atheromatic changes that made them unsuitable for reconstructive surgery and discussed their potential role in tissue destruction. the question arose why bacterial strains inhabiting lower limb skin and considered to be saprophytes become pathogenic once they colonize deep tissues. bacterial pathogenicity is evoked by activation of mult ... | 2016 | 26431369 |
| lethal bacterial trap: cationic surface for endodontic sealing. | insoluble antibacterial cationic nanoparticles have been previously shown to have potent and long-lasting antibacterial properties. our tested hypothesis was that root canal pathogens will be attracted to and eliminated when exposed to epoxy resin-based surfaces incorporating cationic nanoparticles. in our research, an epoxy resin-based surface incorporating quaternary ammonium polyethyleneimine (qpei) nanoparticles was evaluated. surface characterization was performed using atomic force microsc ... | 2016 | 26418438 |
| bactericidal effect of er:yag laser combined with sodium hypochlorite irrigation against enterococcus faecalis deep inside dentinal tubules in experimentally infected root canals. | this study evaluated the bactericidal effect of er:yag laser radiation combined with sodium hypochlorite (naocl) irrigation in the treatment of enterococcus faecalis deep inside dentinal tubules. the er:yag laser was activated, respectively, at 0.3, 0.5 and 1.0 w for either 20 or 30 s; 52.5 g l(-1) naocl and normal saline were used for the control groups. root canals before and after treatments were examined using scanning electron microscopy (sem). bacterial reductions both on the root canal wa ... | 2016 | 26645354 |
| effect of gaseous ozone on enterococcus faecalis biofilm-an in vitro study. | the aim of this study was to evaluate the antimicrobial effect of gaseous ozone compared to conventional methods against enterococcus faecalis. | 2016 | 26637463 |
| anti-breast cancer effects of live, heat-killed and cytoplasmic fractions of enterococcus faecalis and staphylococcus hominis isolated from human breast milk. | development of tumour that is resistant to chemotherapeutics and synthetic drugs, coupled with their life-threatening side effects and the adverse effects of surgery and hormone therapies, led to increased research on probiotics' anticancer potentials. the current study investigated the potential of live, heat-killed cells (hkc) and the cytoplasmic fractions (cf) of enterococcus faecalis and staphylococcus hominis as anti-breast cancer agents. mcf-7 cell line was treated with 25, 50, 100 and 200 ... | 2016 | 26659392 |
| on-chip isothermal nucleic acid amplification on flow-based chemiluminescence microarray analysis platform for the detection of viruses and bacteria. | this work presents an on-chip isothermal nucleic acid amplification test (inaat) for the multiplex amplification and detection of viral and bacterial dna by a flow-based chemiluminescence microarray. in a principle study, on-chip recombinase polymerase amplification (rpa) on defined spots of a dna microarray was used to spatially separate the amplification reaction of dna from two viruses (human adenovirus 41, phi x 174) and the bacterium enterococcus faecalis, which are relevant for water hygie ... | 2016 | 26624222 |
| virulence factors, antimicrobial resistance pattern and molecular analysis of enterococcal strains isolated from burn patients. | the enterococci are emerging as a significant cause of hospital acquired infections. the pathogenesis of enterococci is attributed to the production of virulence factors and resistance to antibiotics. the purpose of the study was to assess the prevalence of genes encoding virulence factor, antimicrobial resistance determinant and molecular characteristic of enterococci isolated from burn patients. a total of 57 enterococci isolated from wound specimens of patients with burn injury were character ... | 2016 | 26620079 |
| screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil. | lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ... | 2016 | 26608755 |
| effect of a benzalkonium chloride surfactant-sodium hypochlorite combination on elimination of enterococcus faecalis. | the purpose of this investigation was to determine the effect of a sodium hypochlorite-surfactant combination on the removal of enterococcus faecalis from infected teeth. | 2016 | 26577873 |
| effect of antimicrobials used in regenerative endodontic procedures on 3-week-old enterococcus faecalis biofilm. | we evaluated the effect of various antimicrobials used in endodontic regeneration on a 3-week-old enterococcus faecalis biofilm. | 2016 | 26577870 |
| influence of biological fluids in bacterial viability on different hospital surfaces and fomites. | the hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. the present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces commonly present in nosocomial environment. | 2016 | 26577628 |
| enterococcus faecalis attenuates osteogenesis through activation of p38 and erk1/2 pathways in mc3t3-e1 cells. | to explore the role of enterococcus faecalis in the proliferation, apoptosis and differentiation of osteoblasts. | 2016 | 26572053 |
| 1h, 15n and 13c chemical shift assignment of the gram-positive conjugative transfer protein trahpip501. | conjugative transfer of dna represents the most important transmission pathway in terms of antibiotic resistance and virulence gene dissemination among bacteria. trah is a putative transfer protein of the type iv secretion system (t4ss) encoded by the gram-positive (g+) conjugative plasmid pip501. this molecular machine involves a multi-protein core complex spanning the bacterial envelope thereby serving as a macromolecular secretion channel. here, we report the near complete (1)h, (13)c and (15 ... | 2016 | 26559076 |
| establishment of a multi-species biofilm model to evaluate chlorhexidine efficacy. | chronic infections, for example, diabetic foot ulcers, have a large impact in terms of patient morbidity and mortality. these wounds are characterized by complex polymicrobial communities of bacteria, which may include a number of difficult-to-eradicate multidrug-resistant pathogens. | 2016 | 26597632 |
| implant placement in failed endodontic sites: a review. | dental implants may fail to osseointegrate in sites of endodontic failure. this may occur as a result colonization by various anaerobic and facultative bacterial species. if an implant is placed in a site where vegetative bacteria are residing, the implant may fail to integrate if a bacterial colonization proceeds coronally. if the implant apical cortical bone is thin or if there is an apical fenestration, the colonization may proceed through the thin or nonexistent bone through the covering muc ... | 2016 | 26550925 |
| daptomycin antibiotic lock therapy for hemodialysis patients with gram-positive bloodstream infections following use of tunneled, cuffed hemodialysis catheters: retrospective single center analysis. | catheter-related blood stream infection (crbsi) is a major complication in hemodialysis patients. we assessed the efficacy of systemic daptomycin (dpt) plus dpt antibiotic lock therapy (dpt-alt) for catheter salvage in patients with gram-positive crbsis. this is a retrospective study of hemodialysis patients with tunneled and cuffed hemodialysis catheters. all patients were from a single institution in taipei and received systemic dpt plus dpt-alt for the treatment of gram-positive crbsi. succes ... | 2016 | 26549513 |
| bioconversion of methanol to value-added mevalonate by engineered methylobacterium extorquens am1 containing an optimized mevalonate pathway. | methylotrophic biosynthesis using methanol as a feedstock is a promising and attractive method to solve the over-dependence of the bioindustry on sugar feedstocks derived from grains that are used for food. in this study, we introduced and engineered the mevalonate pathway into methylobacterium extorquens am1 to achieve high mevalonate production from methanol, which could be a platform for terpenoid synthesis. we first constructed a natural operon (mve) harboring the mvas and mvae genes from en ... | 2016 | 26521242 |
| the native plasmid pml21 plays a role in stress tolerance in enterococcus faecalis ml21, as analyzed by plasmid curing using plasmid incompatibility. | to investigate the role of the native plasmid pml21 in enterococcus faecalis ml21's response to abiotic stresses, the plasmid pml21 was cured based on the principle of plasmid incompatibility and segregational instability, generating e. faecalis mutant strain ml0. the mutant and the wild strains were exposed to abiotic stresses: bile salts, low ph, h2o2, ethanol, heat, and nacl, and their survival rate was measured. we found that curing of pml21 lead to reduced tolerance to stress in e. faecalis ... | 2016 | 26519343 |
| antibacterial effect of n-acetylcysteine and taurolidine on planktonic and biofilm forms of enterococcus faecalis. | this study investigated the antimicrobial activity of taurolidine and n-acetylcysteine (nac) on planktonic and biofilm enterococcus faecalis phenotypes. | 2016 | 26515652 |
| abutment coating with diamond-like carbon films to reduce implant-abutment bacterial leakage. | the influence of diamond-like carbon (dlc) films on bacterial leakage through the interface between abutments and dental implants of external hexagon (eh) and internal hexagon (ih) designs was evaluated. | 2016 | 26513267 |
| antimicrobial effect of the extracts from hypericum perforatum against oral bacteria and biofilm formation. | one traditional medicines, hypericum perforatum l. (hypericaceae), possesses several beneficial effects against depression, ulcers, dyspepsia, abdominal pains, burns, bacterial infections, migraine headaches, and sciatica. | 2016 | 26510970 |
| asymptomatic bacteriuria in clinical urological practice: preoperative control of bacteriuria and management of recurrent uti. | asymptomatic bacteriuria (abu) is a common clinical condition that often leads to unnecessary antimicrobial use. the reduction of antibiotic overuse for abu is consequently an important issue for antimicrobial stewardship and to reduce the emergence of multidrug resistant strains. there are two issues in everyday urological practice that require special attention: the role of abu in pre-operative prophylaxis and in women affected by recurrent urinary tract infections (rutis). nowadays, this is t ... | 2016 | 26742080 |
| early gut colonization of preterm infants: effect of enteral feeding tubes. | the aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates. | 2016 | 26741949 |
| redefining healthy urine: a cross-sectional exploratory metagenomic study of people with and without bladder dysfunction. | we used the pathoscope platform to perform species level analyses of publicly available, 16s rrna pyrosequenced, asymptomatic urine data to determine relationships between microbiomes, and clinical and functional phenotypes. | 2016 | 26807926 |
| monitoring the effectiveness of photodynamic therapy with periodic renewal of the photosensitizer on intracanal enterococcus faecalis biofilms. | photodynamic therapy (pdt) can eliminate microorganisms in a root canal. however, the parameters for disinfection remain undefined. this study assessed the effectiveness of a pdt protocol against intracanal enterococcus faecalis biofilms. | 2016 | 26796030 |
| vinegar treatment prevents the development of murine experimental colitis via inhibition of inflammation and apoptosis. | this study investigated the preventive effects of vinegar and acetic acid (the active component of vinegar) on ulcerative colitis (uc) in mice. vinegar (5% v/v) or acetic acid (0.3% w/v) treatment significantly reduced the disease activity index and histopathological scores, attenuated body weight loss, and shortened the colon length in a murine experimental colitis model induced by dextran sulfate sodium (dss). further mechanistic analysis showed that vinegar inhibited inflammation through supp ... | 2016 | 26795553 |
| in silico driven design and synthesis of rhodanine derivatives as novel antibacterials targeting the enoyl reductase inha. | here, we report on the design, synthesis, and biological evaluation of 4-thiazolidinone (rhodanine) derivatives targeting mycobacterial tuberculosis (mtb) trans-2-enoyl-acyl carrier protein reductase (inha). compounds having bulky aromatic substituents at position 5 and a tryptophan residue at position n-3 of the rhodanine ring were the most active against inha, with ic50 values ranging from 2.7 to 30 μm. the experimental data showed consistent correlations with computational studies. their anti ... | 2016 | 26730986 |
| diversity and evolution of the tn5801-tet(m)-like integrative and conjugative elements among enterococcus, streptococcus, and staphylococcus. | this work describes the diversity and evolution of tn5801 among enterococci, staphylococci, and streptococci based on analysis of the 5,073 genomes of these bacterial groups available in gene databases. we also examined 610 isolates of enterococcus (from 10 countries, 1987 to 2010) for the presence of this and other known ctn-tet(m) elements due to the scarcity of data about tn5801 among enterococci. genome location (by iceu-i-pulsed-field gel electrophoresis [pfge] hybridization/integration sit ... | 2016 | 26729505 |