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ehrlichiosis in brazil.ehrlichiosis is a disease caused by rickettsial organisms belonging to the genus ehrlichia. in brazil, molecular and serological studies have evaluated the occurrence of ehrlichia species in dogs, cats, wild animals and humans. ehrlichia canis is the main species found in dogs in brazil, although e. ewingii infection has been recently suspected in five dogs. ehrlichia chaffeensis dna has been detected and characterized in mash deer, whereas e. muris and e. ruminantium have not yet been identifie ...201121439224
ehrlichia canis (jaboticabal strain) induces the expression of tnf-+¦ in leukocytes and splenocytes of experimentally infected dogs.canine ehrlichiosis is caused by the bacterium ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. this study evaluated the expression of cytokines tnf-+¦, il-10, ifn-+¦, in splenic cells and blood leukocytes during the acute phase of ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the e. canis jaboticabal strain. the study results showed a significant expression of tnf-+¦ 18 days post-inoculation, reducing by ...201121439237
exposure to infectious agents in dogs in remote coastal british columbia: possible sentinels of diseases in wildlife and humans.ranked among the top threats to conservation worldwide, infectious disease is of particular concern for wild canids because domestic dogs (canis familiaris) may serve as sources and reservoirs of infection. on british columbia's largely undeveloped but rapidly changing central and north coasts, little is known about diseases in wolves (canis lupus) or other wildlife. however, several threats exist for transfer of diseases among unvaccinated dogs and wolves. to gain baseline data on infectious ag ...201121461190
serum acute phase proteins as clinical phase indicators and outcome predictors in naturally occurring canine monocytic ehrlichiosis.background: canine monocytic ehrlichiosis (cme), caused by ehrlichia canis, is an important tick-borne disease of global importance. currently, limited information is available on the diagnostic and prognostic value of acute phase proteins (apps) in dogs naturally infected with e. canis. hypothesis: apps may be useful indicators of the clinical phase of cme and predictive of the clinical outcome (death or survival). animals: fifty-six dogs naturally infected with e. canis and 7 clinically health ...201121564293
canine vector-borne disease in travelled dogs in germany-a retrospective evaluation of laboratory data from the years 2004-2008.when importing dogs from various mediterranean countries into western europe canine vector-borne infections are often considered as a major issue. several diseases including babesiosis, leishmaniosis, hepatozoonosis, canine heartworm disease or ehrlichiosis can potentially be endemic in this region and pose a potential health risk for travelling dogs. information on such infections in travelled dogs is scarce and therefore this study has been undertaken to examine the frequency of vector-borne i ...201121565447
tyrosine phosphorylated ehrlichia chaffeensis and ehrlichia canis tandem repeat orthologs contain a major continuous cross-reactive antibody epitope in lysine-rich repeats.a small subset of major immunoreactive proteins have been identified in ehrlichia chaffeensis and ehrlichia canis, including three molecularly and immunologically characterized pairs of immunoreactive tandem repeat protein (trp) orthologs with major continuous species-specific epitopes within acidic tandem repeats (tr) that stimulate strong antibody responses during infection. in this study, we identified a fourth major immunoreactive tr-containing ortholog pair and defined a major cross reactiv ...201121606187
comparative evaluation of a point-of-care immunochromatographic test snap 4dx with molecular detection tests for vector-borne canine pathogens in hong kong.abstract there are no comprehensive studies on the performance of commonly used point-of-care diagnostic enzyme immunoassay for common arthropod-borne canine pathogens. a comparative evaluation of an immunochromatographic test for these infections with a comprehensive polymerase chain reaction (pcr) test panel was performed on 100 pet dogs and 100 stray dogs without obvious clinical symptoms. of the 162 positive test results from both immunochromatographic test and pcr, there was 85.2% concordan ...201121612526
tick-borne bacteria in free-living jaguars (panthera onca) in pantanal, brazil.abstract tick-borne bacteria were investigated in 10 free-living jaguars and their ticks in the pantanal biome, brazil. jaguar sera were tested by indirect fluorescent antibody assays using rickettsia rickettsii, rickettsia parkeri, rickettsia amblyommii, rickettsia rhipicephali, rickettsia felis, rickettsia bellii, ehrlichia canis, and coxiella burnetii as crude antigens. all 10 jaguar sera reacted (titer ≥64) to at least one rickettsia species; 4 and 3 sera reacted with e. canis and c. burneti ...201121612532
rickettsia, ehrlichia, anaplasma, and bartonella in ticks and fleas from dogs and cats in bangkok.abstract flea and tick specimens (5-10 fleas or ticks) on dogs and cats from various sites in bangkok were tested by polymerase chain reaction and dna sequencing to detect dna of bacteria rickettsia (glta and 17 kda genes), anaplasmataceae (16s rrna gene), and bartonella (pap31 and its genes). we confirmed that rickettsia sp. related to rickettsia felis was detected in 66 of 98 (67.4%) flea specimens from dogs, whereas 8 bartonella henselae and 2 bartonella clarridgeiae were detected in 10 of 54 ...201121612535
a glass bead protocol for recovery of host cell free ehrlichia canis and quantification by sybr-green real-time pcr.e. canis infection of the canine cell line dh82 is a routine in studies with this bacteria. a protocol for isolation of host cell free bacteria was developed based on the use of glass beads. improvement of infection with e. canis isolated by this method was detected by real-time pcr.201121667670
babesia canis rossi infection in a texas dog.a 5-month-old intact male boerboel dog, imported from south africa 1 week previously, was presented to a texas veterinarian for lethargy, anorexia, and labored breathing. the dog was febrile, anemic, leukopenic, thrombocytopenic, and slightly azotemic. results of the idexx snap-4dx enzyme immunoassay were negative for dirofilaria immitis antigen and antibodies against ehrlichia canis, borrelia burgdorferi, and anaplasma phagocytophilum. an edta blood sample analyzed at oklahoma state university ...201121790699
a survey of canine tick-borne diseases in india.abstract: background: there are few published reports on canine babesia, ehrlichia, anaplasma, hepatozoon and haemotropic mycoplasma infections in india and most describe clinical disease in individual dogs, diagnosed by morphological observation of the microorganisms in stained blood smears. this study investigated the occurrence and distribution of canine tick-borne disease (tbd) pathogens using a combination of conventional and molecular diagnostic techniques in four cities in india. results: ...201121771313
molecular survey of anaplasma phagocytophilum and ehrlichia canis in red foxes (vulpes vulpes) from central italy.during the 2007-2008 hunting season, 150 spleen samples were collected from free-ranging red foxes (vulpes vulpes) in central italy. the specimens were tested by two nested pcr assays to detect dna of anaplasma phagocytophilum, etiologic agent of granulocytic ehrlichiosis of animals and humans, and dna of ehrlichia canis, which causes the monocytic ehrlichiosis in canids. none of the foxes were pcr-positive for e. canis; 25 (16.6%) were positive for a. phagocytophilum. no specific gross alterati ...201121719836
spatial distribution of seroprevalence for anaplasma phagocytophilum, borrelia burgdorferi, ehrlichia canis, and dirofilaria immitis in dogs in washington, oregon, and california.background: in the us little spatially defined information regarding exposure to most vector-borne pathogens in dogs is available for the states of california (ca), oregon (or), and washington (wa). objectives: the purpose of the present study was to evaluate the spatial distribution of seroprevalence for 4 vector-borne pathogens, anaplasma phagocytophilum, borrelia burgdorferi, ehrlichia canis, and dirofilaria immitis, across the 3 western coastal states of the contiguous united states that ext ...201121827514
the occurrence of dirofilaria immitis, borrelia burgdorferi, ehrlichia canis and anaplasma phagocytophium in dogs in china.a survey of the occurrence of dirofilaria immitis, borrelia burgdorferi, ehrlichia canis and anaplasma phagocytophium in dogs was undertaken in the people's republic of china between october 2008 and october 2009. a total of 600 blood samples were taken from dogs in four cities in china: 300 in beijing, 150 in shenzhen, 30 in shanghai and 120 in zhengzhou. all samples were tested for the heartworm antigen and antibodies of canine b. burgdorferi, e. canis and a. phagocytophium by using the canine ...201121729390
bilateral pelvic lymphadenectomy and radical retropubic prostatectomy for stage c or d1 adenocarcinoma of the prostate: possible beneficial effect of adjuvant treatment.limited clinical stage c (t3 nx m0) disease can be treated surgically, and morbidity can be acceptable. when appropriate adjuvant therapy (orchiectomy and/or radiation) is administered, residual cancer can be controlled locally for at least a limited period. the incidence of local progression in pathologic stage c or d1 disease may be negligible after early adjuvant orchiectomy and/or radiation treatment. the combination of immediate orchiectomy and radical prostatectomy has been shown to limit ...20113173496
bladder cancer among workers in the textile industry: results of a spanish case-control study.this paper presents results from a case-control study carried out in the county of mataro, spain. the study was designed to investigate the possible causes of an unusually high mortality rate from bladder cancer in mataro county as compared to spain as a whole, and this report focuses on occupational exposures. the study is based on 57 cases who were hospitalized for or died from bladder cancer between 1978 and 1981. two controls per case were matched for sex, age, residence, and date of either ...20113232687
correlations of serum potassium fluctuations with body temperature after major surgery.we have observed an association between hypothermia and hypokalemia in a number of postoperative patients. in order to analyze the incidence and consequences of this correlation, 108 patients undergoing major operations were prospectively studied. rectal temperature, serum and urinary potassium, arterial blood gases, blood glucose, and routine monitoring were analyzed. hypothermia occurred in 35 (32%) patients, of whom 20 (57%) suffered from hypokalemia. hypothermia was accompanied by an underco ...20113816276
factors affecting the continued employment of nurses in a state-supported mental health system. 20113856634
fused maxillary second and third molars: report of a rare case with literature review. 20113078009
[fluoride prevention--concerning the health risk]. 20113090743
[better integration of the student into the activities of the ward. goal-oriented integration during the first week is of decisive importance]. 20113090364
[transient one-sided blindness]. 20113187278
acute traumatic spinal cord injury surveillance--united states, 1987. 20113129643
a six-year combined b.a./d.d.s. degree curricular program. 20113162925
retropharyngeal hematoma: complication of cervical fracture. 20113083373
bupropion in depression. i. biochemical effects and clinical response.we studied the biochemical effects of bupropion hydrochloride, a unicyclic antidepressant, in 11 depressed patients. plasma homovanillic acid level increased significantly in patients who had poor responses to treatment but not in patients who obtained good clinical responses. although bupropion is characterized preclinically as a weak dopamine reuptake inhibitor without appreciable effects on norepinephrine (ne) reuptake, it reduced whole-body ne turnover without altering plasma ne levels at re ...20113122698
[poisoning by hydroxyquinoline sulfate in preparing for barium enema]. 20113147547
empirically derived symptom checklist 90 subgroups of chronic pain patients: a cluster analysis.four hundred fifty-three chronic pain patients completed a symptom checklist 90 (scl-90) and a comprehensive pain evaluation questionnaire. all patients were evaluated by a physician and rated on degree of pain pathology and pain behavior. the scl-90 data were analyzed using two clustering procedures and replicated over two similar samples. three distinct profiles emerged and represented high, medium, and low scores on the scl-90. no differences were found between subgroups on demographic charac ...20113172188
use of the 'extended activities of daily living scale' with depressed stroke patients.the 'extended activities of daily living scale' was given to 44 depressed stroke patients. all were more than 1 year after stroke. the completed forms were then guttman scaled to find whether the ranking of the items was valid in this group of patients. the items were not passed in the order expected from the ranking. the results therefore suggest that all items should be assessed when using this scale with depressed stroke patients.20113235384
[adp ribosylation: knowledge and perspectives].polyadenosine diphosphate ribose (polyadpr) and polyadpr-polymerase (polyadpr-p) were discovered in our laboratory in 1966. then it has been demonstrated that the enzyme and the polymer exist in all eukaryotic cells and catalyze a transfer of adp-ribose moieties to proteins. ever increasing evidence favours an involvement of the enzyme through protein adp-ribosylation in dna duplication, transcription and repair. recently the existence of poly or oligo adp-ribosyl transferases were demonstrated ...20102957029
the repairability of oxidative free radical mediated damage to dna: a review.many dna repair enzyme activities are present in both prokaryotic and eukaryotic organisms. among these are dna exo- and endonucleases and dna glycosylases which remove oxidatively damaged portions of the dna molecule, thereby initiating excision-repair. the existence of these enzymes may be taken as evidence that cellular dna is continuously subject to endogenous oxidative stress. many of the lesions introduced by ionizing and ultraviolet radiation are identical to those introduced into dna by ...20102900272
hepatitis b vaccine with and without polymerised albumin receptors. 20102860351
comparative study of biarison and voltaren in acute lumbar pain and lumbo-ischialgia. 20102964738
mechanisms of autoimmunity. 20102865458
[beta-adrenergic blockers]. 20102876553
plasma beta-endorphin immunoreactivity (pl-beta-ed-ir) in patients with ulcer disease and its management by acute and chronic administration of gastrozepin.the initial level of pl-beta-ed-ir was significantly lowered in a group of 14 patients with gastroduodenal ulcer disease as compared with healthy volunteers (p less than 0.05). immediately after i.m. administration of 20 mg gastrozepin (g) the pl-beta-ed-ir level increased but not significantly. given orally over two weeks, g (50 mg/day) led to a more than doubling of the initial level (p less than 0.05). controls showed no significant changes. a further meaningful change represented the time re ...20102937705
inflammatory pseudotumor of the liver. report of the fine needle aspiration cytologic findings in a case initially misdiagnosed as malignant.the fine needle aspiration (fna) cytologic features are described in a case of inflammatory pseudotumor of the liver (xanthogranuloma), a disease generally regarded as of unknown etiology. the initial fna findings were those of an acute exudative process, with atypical biliary duct epithelium and hepatocytes. these were interpreted as verifying the radiologic impression of a malignancy. histologic study of subsequently resected nodules revealed the true nature of the case. as the lesion evolved, ...20102929226
the smoking gun. 20102857863
benign extraaxial tumors: contrast enhancement with gd-dtpa.meningiomas, acoustic neuromas, and other benign extraaxial tumors have little contrast with adjacent brain tissue on conventional magnetic resonance (mr) images. the contrast enhancement produced by intravenous administration of 0.1 mmol/kg of gadolinium-dtpa in these tumors was measured on t1 mr images. acoustic neuromas showed the greatest enhancement (average, 310%), meningiomas the next greatest (average, 180%), and neurofibromas, glomus tumors, and pituitary microadenomas the least enhance ...20103031727
benign extraaxial tumors: contrast enhancement with gd-dtpa.meningiomas, acoustic neuromas, and other benign extraaxial tumors have little contrast with adjacent brain tissue on conventional magnetic resonance (mr) images. the contrast enhancement produced by intravenous administration of 0.1 mmol/kg of gadolinium-dtpa in these tumors was measured on t1 mr images. acoustic neuromas showed the greatest enhancement (average, 310%), meningiomas the next greatest (average, 180%), and neurofibromas, glomus tumors, and pituitary microadenomas the least enhance ...20103031727
truth, trust, and paternalism.the emphasis on the necessity for fully informed consent in two new books, doctors' dilemmas by m. phillips and j. dawson and whose body is it? by c. faulder, is found to be "lacking in balance" by brewin. the doctor's discretion in dealing with each patient is considered to be the key to determining priorities between paternalism and communication. rigid rules, brewin argues, may not be in the patient's best interest and thus should not be forced on doctors and nurses by "philosophers or expert ...20102863503
effect of exercise training in patients with essential hypertension.the aim of the present study was to examine the effect of regular exercise training in moderate essential hypertension. twenty subjects (one woman and 19 men) trained for 45 minutes three times a week for 12 weeks, to 60% of their maximal heart rate. blood pressure was measured at rest and during a maximal exercise test on bicycle ergometer. all subjects achieved training effect measured as a significant reduction in heart rate at comparable submaximal work level and a significant increase in du ...20102856772
transport of light in tissue in photodynamic therapy. 20102964653
[possibility of the early diagnosis of the suture incompetence of a large intestine anastomosis].radionuclide examinations consisting in intraoperative injection of a nonabsorbed radiopharmaceutical preparation into the lumen of adducting portion of the gut were proposed for the detection of incompetence of anastomosis sutures in the early postoperative period. the investigation was performed in 45 patients. complications on the anastomosis were detected in 7 patients: in 5 patients--anastomositis, in 2 patients--incompetence of the anastomosis sutures, the radiopharmaceutical preparation b ...20102836984
therapeutic drug monitoring. 20102863512
analysis of the early rise in aortic transvalvular gradient after aortic valvuloplasty.the relationship between dynamic changes in aortic valve gradient and left ventricular ejection performance in the early period after successful percutaneous aortic valvuloplasty has not been described in detail. accordingly 20 adult patients with severe symptomatic calcific aortic stenosis underwent first-pass radionuclide angiography and doppler echocardiography before, immediately after, and 2 to 4 days after the valvuloplasty procedure. a significant (p less than 0.001) reduction in peak-to- ...20102916414
metaphyseal dysostosis schmidt type. 20103251840
origin of leukemic relapse after bone marrow transplantation detected by restriction fragment length polymorphism.bone marrow transplantation has become an accepted modality in the treatment of acute leukemia. with this therapy, it is possible to obtain long-term disease-free survival. however, leukemia recurs occasionally. in most cases, leukemic relapse is of recipient origin. there have been several reports, though, of leukemia developing in donor cells. these cases have been limited to instances in which there is an easily identifiable chromosome difference or abnormality, usually a sex chromosome. in t ...20102981254
sonographic diagnosis of a chylous mesenteric cyst. 20103091647
rhesus haemolytic disease treated with high-dose intravenous immunoglobulin. 20102860352
thalidomide, orogenital ulcers, and risk of teratogenicity. 20102857866
a case of wound botulism. 20102882064
t-butyl-4-hydroxyanisole as an inhibitor of tumor cell respiration.the effect of t-butyl-4-hydroxyanisole (bha), a widely used food antioxidant additive, on the culture growth, oxygen consumption, and redox state of some electron carriers of intact ta3 and 786a ascites tumor cells has been studied. bha inhibited culture growth and respiration of these two tumor cell lines, by inhibiting the electron flow through the respiratory chain. experiments to determine its site of action showed that bha did not inhibit noticeably the electron flow through cytochrome oxid ...20102818635
ehrlichia ewingii infection and exposure rates in dogs from the southcentral united states.we used pcr and a novel serologic assay to determine infection and exposure rates to ehrlichia ewingii in dogs from an area of northeast oklahoma and northwest arkansas where amblyomma americanum ticks are abundant. of 143 dogs assayed, 13 (9.1%) harbored e. ewingii by pcr and 64 (44.8%) had antibodies to e. ewingii detected using a peptide-based microtiter plate elisa. dogs were more likely (p=0.001) to be positive by pcr if sampled in august (30.8%) but no association was found between seropos ...201020541322
prevalence of canine ehrlichiosis in perak state, peninsular malaysia.the indirect fluorescent antibody test (ifat) and thin blood smears were conducted to establish the prevalence of ehrlichia canis infection in dogs presented for treatment by pet owners at five private and one government veterinary clinic. results showed that 15% of the dogs were positive for the parasite via ifat, but none using blood smears. however, infected dogs did not show severe clinical symptoms of canine monocytic ehrlichiosis (cme).201020562808
prevention of endemic canine vector-borne diseases using imidacloprid 10% and permethrin 50% in young dogs: a longitudinal field study.canine vector-borne diseases (cvbds) are highly prevalent and increasing in distribution worldwide. a longitudinal study was conducted in southern italy to determine the incidence of and protection against cvbd-causing pathogens in dogs treated with a combination of imidacloprid 10% and permethrin 50% (imper). one hundred eleven autochthonous young dogs were divided into group a (n=63) and group b (n=48), both groups containing dogs positive and negative for one or more cvbd-causing pathogens. a ...201020591573
survey for tick-borne zoonoses in the state of espirito santo, southeastern brazil.blood samples collected from 201 humans, 92 dogs, and 27 horses in the state of espirito santo, brazil, were tested by polymerase chain reaction, indirect immunofluorescence assays, and indirect enzyme-linked immunosorbent assay for tick-borne diseases (rickettsiosis, ehrlichiosis, anaplasmosis, borreliosis, babesiosis). our results indicated that the surveyed counties are endemic for spotted fever group rickettsiosis because sera from 70 (34.8%) humans, 7 (7.6%) dogs, and 7 (25.9%) horses were ...201020595502
sensitivity evaluation of a single-step pcr assay using ehrlichia canis p28 gene as a target and its application in diagnosis of canine ehrlichiosis.the aim of this study was to optimize a pcr assay that amplifies an 843 pb fragment from the p28 gene of ehrlichia canis and compare it with two other pcr methods used to amplify portions of the 16s rrna and dsb genes of ehrlichia. blood samples were collected from dogs suspected of having a positive diagnosis for canine ehrlichiosis. amplification of the p28 gene by pcr produced an 843-bp fragment and this assay could detect dna from one gene copy among 1 billion cells. all positive samples det ...201020624341
ehrlichia canis morulae and dna detection in whole blood and spleen aspiration samples.the aim of this study was to compare the detection of ehrlichia canis morulae and dna by npcr in whole blood and spleen aspiration. the sample included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis. morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears. e. canis dna was detected in 29/40 blood samples. we verified that morulae detection is more efficient in cytological preparations ...201020624346
[seroprevalence anti-ehrlichia canis antibodies in dogs of cuiabá, mato grosso].canine ehrlichiosis is a disease transmitted by ticks rhipicephalus sanguineus and caused by ehrlichia canis, obligatory intracellular bacteria. the present study examined the prevalence of anti-e. canis in 254 dogs from four administrative regions of cuiabá, mato grosso, by indirect immunofluorescence assay. there was a prevalence of 42.5% (108/254) without significant difference between the studied regions. the variables age, breed, sex, habitat, access to rural and ticks were analyzed. the an ...201020624348
canine babesiosis in northern portugal and molecular characterization of vector-borne co-infections.abstract:201020377861
comparison of selected canine vector-borne diseases between urban animal shelter and rural hunting dogs in korea.abstract: a serological survey for dirofilaria immitis, anaplasma phagocytophilum, ehrlichia canis, and borrelia burgdorferi infections in rural hunting and urban shelter dogs mainly from southwestern regions of the republic of korea (south korea) was conducted. from a total of 229 wild boar or pheasant hunting dogs, the number of serologically positive dogs for any of the four pathogens was 93 (40.6%). the highest prevalence observed was d. immitis (22.3%), followed by a. phagocytophilum (18.8% ...201020377869
experimental infection and co-infection of dogs with anaplasma platys and ehrlichia canis: hematologic, serologic and molecular findings.abstract:201020377870
imported and travelling dogs as carriers of canine vector-borne pathogens in germany.abstract:201020377872
severe hepatitis associated with acute ehrlichia canis infection in a dog. 201020384948
prevalence of dirofilaria immitis, ehrlichia canis, and borrelia burgdorferi in pet dogs, racing greyhounds, and shelter dogs in florida.arthropod vectors of canine infectious diseases are present throughout florida. since crowded housing has the potential to bring vectors and infected dogs into close proximity, it is possible that prevalence of infection is higher in intensely housed dogs. in this study, the seroprevalence of dirofilaria immitis, ehrlichia canis, and borrelia burgdorferi in dogs residing in two types of intensive housing, greyhound kennels and animal shelters, was compared to dogs residing in low-intensity housi ...201020399018
serological study of selected vector-borne diseases in shelter dogs in central spain using point-of-care assays.we evaluated the prevalence of selected vector-borne diseases in 131 dogs in an animal shelter in central spain using point-of-care assays (snap 4dx and snap leishmania; idexx laboratories, westbrook, me). the snap 4dx detects dirofilaria immitis (di) antigen and antibodies against ehrlichia canis (ec), borrelia burgdorferi (bb), and anaplasma phagocytophylum (aph); the snap leishmania kit detects antibodies against leishmania infantum (li). dogs were classified as healthy or sick based on physi ...201020420531
detection and characterization of four novel genotypes of ehrlichia canis from dogs.the genetic diversity of ehrlichia canis strains worldwide is currently poorly defined. the present study aimed to characterize e. canis strains in naturally infected dogs in taiwan, using a combination of pcr and sequence analysis of the 16s rdna and two antigen-encoding genes, gp19 and gp36. genomic dna was extracted from 34 parasitemic dogs and the genes of the pathogen were separately amplified, sequenced, and aligned with corresponding sequences available in genbank. all 16s rdna sequences ...201020451333
high prevalence of tick-borne pathogens in dogs from an indian reservation in northeastern arizona.we evaluated the serological and molecular prevalence of selected organisms in 145 dogs during late spring (may/june) of 2005 and in 88 dogs during winter (february) of 2007 from the hopi indian reservation. additionally, in 2005, 442 ticks attached to dogs were collected and identified as rhipicephalus sanguineus. infection with or exposure to at least one organism was detected in 69% and 66% of the dogs in may/june 2005 and february 2007, respectively. exposure to spotted fever group (sfg) ric ...201019469667
predominance of ehrlichia chaffeensis in rhipicephalus sanguineus ticks from kennel-confined dogs in limbe, cameroon.rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for ehrlichial agents (ehrlichia canis, e. chaffeensis, and e. ewingii) using a species-specific multicolor real-time taqman pcr amplification of the disulphide bond formation protein (dsb) gene. ehrlichia chaffeensis dna was detected in 33 (56%) ticks, e. canis dna was detected in four (6%) ticks, and one tick was coinfected. the e. chaffeensis and e. canis nucleotide ...201019693681
absence of myelofibrosis in dogs with myelosuppression induced by ehrlichia canis infection.bone marrow (bm) pathology was assessed in 10 dogs with ehrlichia canis-induced aplastic pancytopenia. bm core biopsy sections were stained with haematoxylin and eosin and with haematoxylin/van gieson and gordon and sweets' reticulin stain for the detection of collagen and reticulin fibres, respectively. iron stores were assessed by perls' prussian blue staining. there was no significant deposition of collagen or reticulin in any sample, but in seven dogs the bm was depleted of haemosiderin. the ...201019906384
application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples.abstract:201020704695
first isolation and molecular characterization of ehrlichia canis in costa rica, central america.the present study investigated ehrlichia species in blood samples from dogs suspected of clinical ehrlichiosis, using molecular and isolation techniques in cell culture. from a total of 310 canine blood samples analyzed by 16s rrna nested pcr, 148 (47.7%) were positive for ehrlichia canis. dna from ehrlichia chaffeensis or ehrlichia ewingii was not detected in any sample using species-specific primers in separated reactions. leukocytes from five pcr-positive dogs were inoculated into dh82 cells; ...201020723954
pathogens and symbionts in ticks: a survey on tick species distribution and presence of tick-transmitted micro-organisms in sardinia, italy.a total of 1485 adult ticks were collected from mammalian hosts in south-eastern sardinia, italy, during the years 2007-2008. ticks were identified and tested by pcr analysis for presence of rickettsia species of the spotted fever group, ehrlichia canis, anaplasma phagocytophilum, coxiella burnetii, bartonella species and leishmania species. among all tick species examined (rhipicephalus sanguineus, rhipicephalus turanicus, rhipicephalus bursa, rhipicephalus pusillus, hyalomma marginatum margina ...201020884769
evaluation of peptide- and recombinant protein-based assays for detection of anti-ehrlichia ewingii antibodies in experimentally and naturally infected dogs. [corrected]to evaluate microtiter-plate format elisas constructed by use of different diagnostic targets derived from the ehrlichia ewingii p28 outer membrane protein for detection of e ewingii antibodies in experimentally and naturally infected dogs.201020919906
efficacy of a doxycycline treatment regimen initiated during three different phases of experimental ehrlichiosis.doxycycline is the treatment of choice for canine monocytic ehrlichiosis (cme), a well-characterized disease and valuable model for tick-borne zoonoses. conflicting reports of clearance of ehrlichia canis after treatment with doxycycline suggested that the disease phase during which treatment is initiated influences outcomes of these treatments. the purpose of this study was to evaluate the efficacy of a 28-day doxycycline regimen for clearance of experimental e. canis infections from dogs treat ...201020921310
ehrlichiosis and anaplasmosis in dogs and cats.in the time since canine ehrlichiosis due to ehrlichia canis was first described in 1935 and first recognized in the united states in 1962, many key advances have been made in our understanding of the diversity of the rickettsial organisms responsible for ehrlichiosis and anaplasmosis in dogs and, occasionally, cats, the vectors capable of transmitting these agents, and the role these organisms play as both important veterinary pathogens and zoonotic disease agents. despite considerable progress ...201020933140
validation of a leishmania infantum elisa rapid test for serological diagnosis of leishmania chagasi in dogs.canine visceral leishmaniasis (cvl) is caused by leishmania donovani complex parasites including l. donovani, leishmania infantum and leishmania chagasi. as some studies suggest that l. chagasi and l. infantum may be very similar or even the same species, the aim of the present study was to evaluate a commercial rapid elisa test, originally designed for l. infantum, in the diagnosis of cvl in dogs naturally infected by l. chagasi. a total of 400 serum canine samples, including 283 positive dogs ...201021030153
evolution of clinical, haematological and biochemical findings in young dogs naturally infected by vector-borne pathogens.longitudinal studies evaluating the evolution of clinical, haematological, biochemical findings in young dogs exposed for the first time to multiple vector-borne pathogens have not been reported. with the objective of assessing the evolution of clinical, haematological and biochemical findings, these parameters were serially monitored in naturally infected dogs throughout a 1-year follow-up period. young dogs, infected by vector-borne pathogens based on cytology or polymerase chain reaction, wer ...201021106311
effect of desmopressin on immune-mediated haemorrhagic disorders due to canine monocytic ehrlichiosis: a preliminary study.to evaluate the possible use of desmopressin acetate (ddavp) in haemorrhagic disorders consequent to canine monocytic ehrlichiosis (cme), three dogs infected by ehrlichia canis, with a history of thrombocytopenia and recent bleeding, were studied. the dogs were administered desmopressin (1 μg/kg b.w. s.c.) every 24 h on three occasions. blood samples were collected immediately before, and after 2 and 48 h the first ddavp administration, to assess haematological, clinical chemistry and clotting t ...201021108506
sequence and phylogenetic analysis of the gp200 protein of ehrlichia canis from dogs in taiwan.ehrlichia (e.) canis is a gram-negative obligate intracellular bacterium responsible for canine monocytic ehrlichiosis. currently, the genetic diversity of e. canis strains worldwide is poorly defined. in the present study, sequence analysis of the nearly full-length 16s rdna (1,620 bp) and the complete coding region (4,269 bp) of the gp200 gene, which encodes the largest major immunoreactive protein in e. canis, from 17 taiwanese samples was conducted. the resultant 16s rdna sequences were foun ...201021113103
performance of a commercially available in-clinic elisa for the detection of antibodies against anaplasma phagocytophilum, ehrlichia canis, and borrelia burgdorferi and dirofilaria immitis antigen in dogs.to evaluate the sensitivity and specificity of a commercially available in-clinic elisa for detection of heartworm infection and tick-borne diseases in dogs.201021117995
strong strand composition bias in the genome of ehrlichia canis revealed by multiple methods.genes located on the two replicating strands are found to have two separate base/codon usages in ehrlichia canis genome. although strand-specific codon usage is not the first observation, for the first time we have applied multiple methods to the analysis of strand composition bias. by combining multiple methods, comprehensive and interesting results are obtained. among three types of correspondence analysis (coa), absolute codon usages between genes on the two replicating strands are more disti ...201021253460
diagnosis of canine vector-borne diseases in young dogs: a longitudinal study.canine vector-borne diseases (cvbds) pose a diagnostic challenge, particularly when a dog is coinfected with more than one pathogen. the purpose of this study was to generate information about the diagnosis of cvbds in young dogs following their first exposure to flea, tick, sand fly, louse, and mosquito vectors. from march 2008 to may 2009, 10 purpose-bred young naive beagle dogs and a cohort of 48 mixed-breed dogs living in an area to which cvbd is endemic in southern italy were monitored usin ...201020660218
a novel ehrlichia genotype detected in naturally infected cattle in north america.during a research investigation to determine if cattle from british columbia (bc), canada were infected with anaplasma marginale or other related rickettsial blood parasites, a novel ehrlichia genotype was revealed. blood from seven bc source cattle was bioassayed by intravenous inoculation into naïve splenectomised calves. additional splenectomised calves were used as uninoculated negative control or a. marginale-inoculated positive control. newly designed sets of primers specific for the msp5 ...201020663613
multiplex real-time qpcr for the detection of ehrlichia canis and babesia canis vogeli.ehrlichia canis and babesia canis vogeli are two tick-borne canine pathogens with a worldwide importance. both pathogens are transmitted by rhipicephalus sanguineus, the brown dog tick, which has an increasing global distribution. a multiplex quantitative real-time pcr (qpcr) assay for the simultaneous detection of the tick-borne pathogens e. canis and b. canis vogeli was developed using dual-labeled probes. the target genes were the 16s rrna of e. canis and the heat shock protein 70 (hsp70) of ...201020674177
molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan recife (north-eastern brazil).to identify dna of the main tick-borne pathogens in dogs from recife (brazil), polymerase chain reactions were carried out on blood samples of dogs treated at the veterinary hospital of the universidade federal rural de pernambuco from march 2007 to june 2008. the detection of dna was performed using specific primers. amplicons were analyzed through electrophoresis and sequencing. a phylogenetic tree was constructed using the upgma method, revealing that the sequences were closely related to tho ...201020680344
molecular and serologic detection of ehrlichia spp. in endangered brazilian wild captive felids.ehrlichiosis, an emergent tick-borne disease that affects both humans and animals, may represent a threat to the survival and preservation of wild felids in brazil. there are few studies of ehrlichiosis in wild felids in brazil, but ehrlichia spp. are present in domestic cats. antibodies to ehrlichia canis have been reported in a puma (puma concolor). in this study we assessed the presence of these hemoparasites in the blood of brazilian wild captive felids. of the 72 animals tested, 5 (7%) were ...201020688716
molecular detection of anaplasma platys and ehrlichia canis in dogs from the north of portugal.tick-transmitted rickettsial pathogens belonging to the ehrlichia and anaplasma genera can infect dogs and humans. in this study, four dogs from the north of portugal, in which an ehrlichial disease was suspected clinically, were tested by molecular methods. after dna extraction from blood on filter paper, a 345 bp fragment of the ehrlichia/anaplasma 16s rrna gene was amplified by the polymerase chain reaction (pcr). sequence analysis of pcr products revealed one dog infected with ehrlichia cani ...201019056304
the increasing recognition of rickettsial pathogens in dogs and people.dogs and people are exposed to and susceptible to infection by many of the same tick-borne bacterial pathogens in the order rickettsiales, including anaplasma phagocytophilum, ehrlichia canis, e. chaffeensis, e. ewingii, rickettsia rickettsii, r. conorii, and other spotted fever group rickettsiae. recent findings include descriptions of novel ehrlichia and rickettsia species, recognition of the occurrence and clinical significance of co-infection, and increasing awareness of rhipicephalus sangui ...201020207197
sce induction by ccnu and pregnancy. 20092704378
detection of babesia canis subspecies and other arthropod-borne diseases in dogs from tirana, albania.summary. the importance of arthropod-borne diseases increased in the recent past in particular due to frequent travel with dogs in or by importing of dogs from regions with endemic occurrence of these diseases. while the epidemiological situation is well known for the western parts of the mediterranean, only limited data is available for eastern europe and the balkans. thirty clinically healthy dogs from suburban areas of tirana, albania, were tested for babesia canis, hepatozoon spp., leishmani ...200919915816
[comparison of nested-pcr with blood smear examination in detection of ehrlichia canis and anaplasma platys in dogs].the clinical signs of ehrlichia canis and anaplasma platys infection are similar, and the diagnosis of these pathogens made by stained blood smears is poor due sensibility and specificity. on the other hand, the molecular diagnosis is highly sensitive and specific and nested-pcr have been optimized for accurate diagnosis these pathogens in dogs. at the veterinary teaching hospital, whole-blood samples with edta were obtained from 100 dogs and smears were made from blood samples for evaluation fo ...200920040193
ticks and haemoparasites of dogs from praia, cape verde.in february 2008 an epidemiological field study on arthropod-borne infections in dogs was carried out in praia, the capital city of cape verde. for this purpose 130 dogs were included in the study. of these, 94.6% were infested with ticks. altogether, 1293 ticks of the genus rhipicephalus (in all evaluated cases r. sanguineus) were collected. examination for haemotropic parasites was performed via polymerase chain reaction (pcr). lymph node fine-needle aspirates were screened by pcr for leishman ...200919729247
[ehrlichia canis in dogs attended in a veterinary hospital from botucatu, são paulo state, brazil].this study investigated the etiology of canine ehrlichiosis and possible clinical and epidemiological data associated with the infection in 70 dogs suspect of ehrlichiosis attended at the veterinary hospital of the são paulo state university in botucatu city during 2001 and 2002. dogs were evaluated by clinical-epidemiological and hematological data and molecular analysis by partial amplification and dna sequencing of the ehrlichial dsb gene. e. canis dna was amplified and sequenced in 28 (40.0% ...200919772777
canine ehrlichiosis in the ivory coast and gabon: alteration of biochemical blood parameters based on ehrlichia canis serology. 200919793128
babesia canis and other tick-borne infections in dogs in central poland.vector-borne infections constitute increasing health problem in dogs worldwide, including sled dogs, dramatically decreasing the fitness of working dogs and even leading to death. in the period 2006-2008 eighty-two blood samples were collected from eight sled dog kennels in central poland. the prevalence of four vector-borne infections (babesia canis, bartonella sp., anaplasma/ehrlichia and borrelia burgdorferi) was estimated in 82 sled dogs using pcr and nested pcr for diagnosis and the same me ...200919837515
an acute severe ehrlichiosis in a dog experimentally infected with a new virulent strain of ehrlichia canis. 200919548986
occurrence of dirofilaria immitis and tick-borne infections caused by anaplasma phagocytophilum, borrelia burgdorferi sensu lato and ehrlichia canis in domestic dogs in france: results of a countrywide serologic survey.the occurrence of dirofilaria immitis antigen and antibodies against tick-borne pathogens in french dogs has been analysed based on 1,050 blood samples. serum samples of 919 dogs (group a) were sent for a variety of diagnostic investigations, further 131 dogs (group b) were tested for a tentative diagnosis of heartworm disease. all samples were tested for d. immitis antigen. samples in group a were also tested for specific antibodies against three tick-borne pathogens (anaplasma phagocytophilum, ...200919575231
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