Publications

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high-pressure liquid chromatographic determination of hydroxy-s-triazine residues in plant material. 2005546953
[infectious endocarditis--a changing disease]. 2005544233
erythema gyratum atrophicans transiens neonatale.an unknown dermatosis was observed in an infant girl. during the newborn period, she had a generalized, patchy, erythematous eruption of about 60 round erythematous patches on the trunk, thighs, head, labial mucosa, and palate. after a few weeks, the lesions became whitish, atrophic, and depressed, and they were surrounded by an erythematous and slightly infiltrated border, which was occasionally lobulated. histologically, the lesions demonstrated a thin epidermis overlying an edematous dermis. ...20051169042
development of gingivitis in pre-school children and young adults. a comparative experimental study.earlier studies suggest that children and adults differ in the propensity to develop gingivitis when oral hygiene is abandoned. to confirm the existence of such a difference, a comparative study of pre-school children and young adults was made with objective registration methods. the author performed all registrations. after a period of intensive oral hygiene, all cleaning of teeth was discontinued for 21 days. the amount of bacterial plaque, the amount of gingival exudate and crevicular leukocy ...2004353084
macular oedema in the reattached retina. 2004525278
increased incidence of cervical cytological abnormalities in women with genital warts or contact with genital warts: a need for increased vigilance?to determine whether women who have a history of genital warts or whose sexual partners have such a history were more likely to have borderline or dyskaryotic cervical smears than other women.20041774050
the disposition of cyclophosphamide in a group of myeloma patients.the disposition of cyclophosphamide and its alkylating metabolites was investigated in a group of myeloma patients with varying degrees of renal function impairment. no correlation between renal function and clearance of cyclophosphamide or its alkylating metabolites was found. no evidence of accumulation of cyclophosphamide or alkylating activity was found in four patients receiving radiolabelled cyclophosphamide. renal function was found to be related to the reciprocal of the area under curve ...2004535136
ghq and psychiatric case. 2004444799
effect of prostaglandin e1 on renal haemodynamics in a patient with diabetic nephropathy.a 66-year old male with severe hyperglycaemia due to previously uncontrolled diabetes mellitus was also suffering from arteriosclerosis obliterans and diabetic nephropathy. the patient was treated with 16 iu/day insulin zinc suspension. in addition, an intravenous infusion of 80 micrograms/day prostaglandin e1 was given for 28 days in an attempt to improve the arteriosclerosis obliterans and diabetic nephropathy. treatment resulted in a reduction in fasting blood glucose but no decline in urinar ...20041773911
biofeedback in the management of partial anal incontinence: a preliminary report.this method of improving anal continence is recommended for patients with partial anal incontinence, in whom some functioning anal sphincter remains. it is particularly applicable for treating those patients who have sustained operative trauma, but is less helpful for those who have generalized sphincter weakness; however, any patient with some sphincter function may benefit. emotionally unstable patients are less likely to benefit. although the series was small, the results were considered suff ...2004446249
[international day of the child]. 2004427841
covert and overt recognition in prosopagnosia.prosopagnosic patients suffer an inability to recognize familiar people by visual inspection of their faces. despite the absence of overt recognition, though, some prosopagnosic patients continue to process the identities of familiar faces covertly. a longstanding controversy concerns whether the recognition deficit in prosopagnosia is specific for faces, or also affects other types of visual stimuli. we investigated whether the patient p.h., who has severe problems with within-class recognition ...20041782532
[how does one calculate the activity of a radiodiagnostic service?]. 2004529236
prognistic relevance of radiation induced immune suppression in breast carcinoma.the extent of radiation induced immune suppression was analysed in 100 patients with carcinoma of the breast. the relative changes of lymphocyte counts and stimulations by pha and ppd were similar in patients who differed with regard to age, size of tumour and its malignancy grade or axillary node condition. moreover, no difference in the degree of radiation induced immune suppression existed between patients who developed recurrent disease and those who remained free of disease during a follow- ...2004525447
prophylactic infusion therapy in hemophilia. 2004478502
genetic analysis of an ambler class a extended-spectrum beta-lactamase from capnocytophaga ochracea.a beta-lactamase gene (cfxa3, 966 bp) was isolated from a beta-lactam-resistant capnocytophaga ochracea clinical isolate and amplified using primers from the cfxa gene of bacteroides vulgatus. the mics of third-generation cephalosporins were much higher than those of the transconjugant escherichia coli strain. the deduced protein sequence, by comparison with cfxa2 of prevotella intermedia, had a y239d substitution and possessed the characteristics of a class a, group 2e beta-lactamase.200414766881
15-deoxy-delta12,14-prostaglandin j2-mediated erk signaling inhibits gram-negative bacteria-induced rela phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2a activity.we have previously shown that non-pathogenic gram-negative bacteroides vulgatus induces transient rela phosphorylation (ser-536), nf-kappab activity, and pro-inflammatory gene expression in native and intestinal epithelial cell (iec) lines. we now demonstrate that 15-deoxy-delta(12,14)-prostaglandin j(2) (15d-pgj(2)) but not prostaglandin e(2) inhibits lipopolysaccharide (lps) (b. vulgatus)/lps (escherichia coli)-induced rela phosphorylation and interleukin-6 gene expression in the colonic epith ...200415199053
characterization of plasmid por1 from ornithobacterium rhinotracheale and construction of a shuttle plasmid.the bacterium ornithobacterium rhinotracheale has been recognized as an emerging pathogen in poultry since about 10 years ago. knowledge of this bacterium and its mechanisms of virulence is still very limited. here we report the development of a transformation system that enables genetic modification of o. rhinotracheale. the system is based on a cryptic plasmid, por1, that was derived from an o. rhinotracheale strain of serotype k. sequencing indicated that the plasmid consisted of 14,787 nucle ...200415466524
experimental swine dysentery: comparison between infection models.the aim of the present study was to develop a reproducible porcine infection model with brachyspira hyodysenteriae. the influence of different factors was evaluated, namely, age, a diet containing large quantities of soybean meal, housing and administration of cortisol or antacids. furthermore, the synergistic effect of additional bacteria (escherichia coli o141, bacteroides vulgatus or a mixture of bacteroides fragilis, a field isolate of bacteroides and fusobacterium necrophorum) was studied. ...200415017282
lipopolysaccharide induces cd25-positive, il-10-producing lymphocytes without secretion of proinflammatory cytokines in the human colon: low md-2 mrna expression in colonic macrophages.despite the huge number of colonized gram-negative bacteria in the colon, the normal colon maintains its homeostasis without any excessive immune response. to investigate the potential mechanisms involved, human colonic lamina propria mononuclear cells (lpmcs) obtained from uninflamed mucosa were cultured with lipopolysaccharide (lps) prepared from bacteroides vulgatus (bv-lps) or bacteroides fragilis (bf-lps), as representatives of indigenous flora, or pathogenic salmonella minnesota (sm-lps). ...200414997033
commensal gram-negative bacteria prime human dendritic cells for enhanced il-23 and il-27 expression and enhanced th1 development.dendritic cells (dc) are the main orchestrators of specific immune responses. depending on microbial information they encounter in peripheral tissues, they promote the development of th1, th2 or unpolarized th cell responses. in this study we have investigated the immunomodulatory effect of non-pathogenic intestinal gram-negative (escherichia coli, bacteroides vulgatus,veillonella parvula, pseudomonas aeruginosa) and gram-positive (bifidobacterium adolescentis, enterococcus faecalis, lactobacill ...200415114670
dna from probiotic bacteria modulates murine and human epithelial and immune function.the intestinal epithelium must discriminate between pathogenic and nonpathogenic bacteria and respond accordingly. the aim of this study was to examine whether bacterial dna can serve as the molecular basis for bacterial recognition.200415131797
differential effect of immune cells on non-pathogenic gram-negative bacteria-induced nuclear factor-kappab activation and pro-inflammatory gene expression in intestinal epithelial cells.we have previously shown that non-pathogenic gram negative bacteria induce rela phosphorylation, nuclear factor (nf)-kappab transcriptional activity and pro-inflammatory gene expression in intestinal epithelial cells (iec) in vivo and in vitro. in this study, we investigated the molecular mechanism of immune-epithelial cell cross-talk on gram-negative enteric bacteria-induced nf-kappab signalling and pro-inflammatory gene expression in iec using ht-29/mtx as well as caco-2 transwell cultures int ...200415147574
molecular inventory of faecal microflora in patients with crohn's disease.intestinal microbial community is involved in the pathogenesis of crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. using sequence analysis of randomly cloned bacterial 16s ribosomal dna, the dominant faecal species from four crohn's disease patients and four controls were compared. whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly re ...200419712374
transforming growth factor-beta 1 inhibits non-pathogenic gram negative bacteria-induced nf-kappa b recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation.we have shown that non-pathogenic enteric gram-negative bacteroides vulgatus induces rela phosphorylation, nf-kappab activation, and proinflammatory gene expression in primary and intestinal epithelial cell (iec) lines. we now demonstrate the transient induction of nuclear phospho-rela (day 3) followed by persistent activation of phospho-smad2 (days 3 and 7) in iec from mucosal tissue sections of b. vulgatus-monoassociated rats, indicating that both nf-kappab and transforming growth factor-beta1 ...200312672795
accelerated alveolar bone loss in mice lacking interleukin-10.interleukin-10 regulates pro-inflammatory cytokines, including those implicated in alveolar bone resorption. we hypothesized that lack of interleukin-10 leads to increased alveolar bone resorption. male interleukin-10(-/-) mice, on 129/svev and c57bl/6j background, were compared with age-, sex-, and strain-matched interleukin-10(+/+) controls for alveolar bone loss. immunoblotting was used for analysis of serum reactivity against bacteria associated with colitis and periodontitis. interleukin-10 ...200312885849
fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats.the effects of fructans in the diet on the mucosal morphometry (height of villi, depth of the crypts, number of goblet cells), the thickness of the epithelial mucus layer and the histochemical composition of intestinal mucosubstances in the distal jejunum and the distal colon were investigated by comparing germ-free (gf) rats, rats harbouring bacteroides vulgatus and bifidobacterium longum (diassociated (da) rats), and rats with a human faecal flora (hfa). the rats were fed either a commercial s ...200312720580
lactobacillus gg prevents recurrence of colitis in hla-b27 transgenic rats after antibiotic treatment.bacteroides vulgatus induces colitis in gnotobiotic hla-b27 transgenic (tg) rats while broad spectrum antibiotics prevent and treat colitis in specific pathogen free (spf) tg rats although disease recurs after treatment ends. lactobacilli treat human pouchitis and experimental colitis. we investigated if lactobacillus rhamnosus gg (l gg) can prevent colitis in tg rats monoassociated with b vulgatus and if l gg or lactobacillus plantarum 299v (lp 299v) can treat established colitis in spf tg rats ...200312584218
the suppressive effect of bifidobacteria on bacteroides vulgatus, a putative pathogenic microbe in inflammatory bowel disease.bacteroides, a predominant commensal bacteria in the gut, are thought to be responsible for the development of inflammatory bowel disease (ibd). in the present study, we examined whether or not bifidobacteria suppress b. vulgatus, a representative pathogenic bacteroides species, in both the coculture system and the gnotobiotic murine model. as a result, bifidobacterium infantis 1222 highly inhibited the growth of b. vulgatus in the coculture and also significantly suppressed the systemic antibod ...200312906096
bacteroides vulgatus protects against escherichia coli-induced colitis in gnotobiotic interleukin-2-deficient mice.the microflora plays a crucial role in inflammatory bowel diseases (ibds). specific pathogen-free (spf), but not germ-free, interleukin (il)-2-deficient (il-2-/-) mice develop colitis. the colitogenicity of commensal bacteria was determined.200312851881
enhancement of lymphocyte and macrophage function in vitro by lithium chloride. 2003149624
[calcium and its relation with pachycurares]. 2003154727
severe orthopedic disability in childhood: solutions provided by rehabilitation engineering. 2003149287
[combined treatment of rheumatological patients at a health resort]. 2003154775
culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients.renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ...200314662931
activities of garenoxacin (bms-284756) and other agents against anaerobic clinical isolates.a total of 590 clinical isolates consisting of 33 species of both gram-positive and gram-negative anaerobes were collected from nine centers in the chicago area in 1998-1999. the largest number of isolates (330 isolates, 56%) belonged to the bacteroides group. isolates were tested by agar dilution against garenoxacin (bms-284756, t-3811 me), trovafloxacin, moxifloxacin, clindamycin, imipenem, piperacillin-tazobactam, and cefoxitin. all but one species (2% of bacteroides vulgatus isolates) were f ...200312604521
prevention of gut inflammation by bifidobacterium in dextran sulfate-treated gnotobiotic mice associated with bacteroides strains isolated from ulcerative colitis patients.indigenous bacteroides strains are closely associated with the occurrence and exacerbation of ulcerative colitis (uc). in this study, we aimed to clarify the effect of bifidobacterium strains, another major member of colonic bacteria, on the development of gut inflammation using gnotobiotic mouse models associated with bacteroides strains isolated from uc patients. dextran sulfate (dss) administration induced inflammation in the large intestine, in particular of the cecum, in the gnotobiotic mic ...200312650769
sequence analysis of cfxa2-like beta-lactamases in prevotella species.sixty-two strains of oral (32) and non-oral (30) prevotella producing beta-lactamases were screened for cfxa by pcr, using an intragenic primer pair. all 62 were cfxa/cfxa2 positive. fourteen of these strains, representing seven pigmented and seven non-pigmented prevotella species were submitted to further pcr with specific primers that amplified the whole beta-lactamase structural gene (966 bp). after cloning and sequencing, the deduced amino acid sequences were compared with that of bacteroide ...200312697645
[intestinal flora and crohn's disease].the pathogenesis of inflammatory bowel diseases (ibd) proceeds through stages of initiation, amplification and healing. abundant clinical and experimental data incriminate luminal bacteria or bacterial products in both the initiation and perpetuation of chronic intestinal inflammation. macrophage and t-cell activation with accompanying inflammatory cytokine production appears to be an early event. studies of lymphocyte responsiveness to autologous and heterologous intestinal bacteria have sugges ...200312843962
emergence of fluoroquinolone resistance among bacteroides species.several newer generation fluoroquinolones have demonstrated good in vitro activity against bacteroides species; particularly when first introduced. however, resistance of bacteroides to quinolones appears to be increasing.200312865399
a new device to support handicapped patients during venography or myelography.the authors describe a seating device which holds the patient in position when the x-ray table is tilted and permits non-weight-bearing venograms to be obtained in handicapped as well as normal patients. simultaneous bilateral venography reduces the study time considerably. the device is also helpful in lumbar myelography in paraplegics.2003149336
[cooperative properties of d-glyceraldehyde-3-phosphate dehydrogenase].the structure of the active center of glyceraldehyde-3-phosphate dehydrogenase and the arrangement of subunits in the tetrameric molecule is delineated. the mechanism of cooperative effects in the oligomer is considered, and the involvement of various regions of the active center and of different-subunit contact area in the realization of the cooperative phenomena is discussed. a special attention is paid to the effect of nad+ bound to one of the subunits of the tetramer on the structure of an a ...2003193581
the formation, structure and analysis of the thermal decomposition product of baclofen. 2003539443
[pressure sensors give a receit for balloon dilatation. do new sensors improve the pacemaker?]. 20031774977
pharmacokinetics of theophylline and its metabolites during acute renal failure. a case report.after unsuccessful therapy with salbutamol syrup and inhaled terbutaline a 3-year-old boy with an acute exacerbation of asthma was treated with nebulised salbutamol (albuterol), intravenous aminophylline and hydrocortisone. his condition continued to deteriorate and he required artificial ventilation. subsequently, he became anuric, with liver dysfunction, nonspecific encephalopathy and limb tremor. peritoneal dialysis was started. plasma theophylline concentrations were monitored and maintained ...20031773552
[diagnostik significance of cell membrane markers in childhood all (author's transl)].acute lymphoblastic leukemia (all) in childhood is not a homogeneous disease. by membrane phenotyping 6 groups of all in 66 children could be differentiated: group i (38%) common all (anti-call+. group ii (32%) common all with t cell marker in addition (anti-call+, anti-t+). group iii (14%) t. cell all with e-rosette marker (anti t+, e+). group iv (9%) t cell all without e-rosette marker (anti t+, e-). group v (6%) undifferentiated all (anti-call-, anti-t). group vi (1%) b cell typ (smig+). 19 o ...2003309025
daily activity of tabanidae in the caucasus.the daily activity of tabanidae (diptera) was studied in the submountain region of the east caucasus, district ismailly, ne azerbaijan ssr at an altitude 900--1000 m. tabanidae were collected by the unbaited canopy trap on 8 and 12 july 1976. females were active from 9 a. m. to 7 p. m., the diurnal biting activity is characterized by a one peak curve with a conspicuous peak of activity between 4 p. m. and 6 p. m. it is suggested that the temperature, relative humidity and the thermic mountain wi ...2003156514
ethics education for psychiatry. 20021773105
[determination of the gc groups in liquid blood by vertical electrophoresis on polyacrylamide gel]. 2002129894
[social condition of handicapped children and adolescents in the care of the health office (author's transl)]. 2002126397
technegas for inhalation lung imaging.technegas, an aerosol generator recently devised in australia, produces aerosol particulates called 'technegas' which have characteristics of both an aerosol and a gas. the majority of the particulate is below 200 nm in size as measured by electron microscopy. four normal subjects and 31 patients with various lung diseases were studied by imaging the lungs following inhalation of technegas. the penetration of inhaled technegas to the lung periphery was excellent; the average alveolar deposition ...20021850822
[tubal ligation using yoon's ring].the use of yoon's ring (a silicone band) to close the fallopian tubes avoids some of the serious complications involved in the coagulation technique, such as hemorrhage and perforation. the ring was implanted in 50 subjects using minilaparotomy for those who could not undergo laparoscopy (3 cases), and laparoscopy with 1 incision for 21 cases and 2 incisions for 26 cases. the patients indicated no severe problems and after a total of 404 months without contraceptives, there were no pregnancies. ...2002134936
low back pain in college football linemen. 2002127076
on the origin of protein biosynthesis.there is a very close steric relationship between the codon-anticodon site which accounts for the genetic code dictionary and a polynucleotide replicase site. protein biosynthesis must therefore have arisen out of a primaeval polynucleotide replicase system.2002126580
[studies on the specificity of the antistreptolysin-reaction (author's transl)]. 2002126540
[radioimmunoassay of thyroxine-binding globulin (author's transl)]. 2002119885
senator bennett: where are you? any success of p.s.r.o. will be credited to him, any failure to organized medicine. 2002124080
the effects of religious backgrounds on emotional problems. 2002126985
interdisciplinary early intervention program.developmental data were obtained on 40 children with down's syndrome by an interdisciplinary team during an ongoing early intervention program. interventive methods are described. results are compared with available data on children with down's syndrome who were not in early intervention programs. comparisons of developmental progress are also made of children begun in the early intervention program before six months of age with those begun after six months of age. conclusions from the study ind ...2002128763
ikk beta and phosphatidylinositol 3-kinase/akt participate in non-pathogenic gram-negative enteric bacteria-induced rela phosphorylation and nf-kappa b activation in both primary and intestinal epithelial cell lines.pathogenic and enteroinvasive bacteria have been shown to trigger the i kappa b/nf-kappa b transcriptional system and proinflammatory gene expression in epithelial cells. in this study, we investigated the molecular mechanism of the commensal gram-negative bacteroides vulgatus-induced nf-kappa b signal transduction in intestinal epithelial cells (iec). we report that b. vulgatus induced interleukin-1 receptor-associated kinase-1 degradation, i kappa b alpha phosphorylation/degradation, rela and ...200212140289
comparison of the faecal microflora of patients with ankylosing spondylitis and controls using molecular methods of analysis.to determine whether differences within the complex intestinal microflora can be demonstrated between patients with ankylosing spondylitis (as) and healthy individuals.200212468819
production and application of new monoclonal antibodies specific for a fecal helicobacter pylori antigen.the aim of the present study was to establish monoclonal antibodies that could be used to produce a diagnostic test composed of one kind of monoclonal antibody recognizing a fecal helicobacter pylori antigen. the need to develop such a test arose from disadvantages of the diagnostic test that uses a polyclonal antibody or plural kinds of monoclonal antibodies, such as the lower specificity for h. pylori antigen and the difficulty of reproduction with consistent quality. mice were immunized with ...200211777832
adhesive property of bifidobacterium lactis lkm512 and predominant bacteria of intestinal microflora to human intestinal mucin.the adhesive property to the intestinal mucin of bifidobacterium lactis lkm512, b. longum, b. breve, b. bifidum, b. adolescentis, b. infantis, bacteroides vulgatus, bacteroides distasonis, eubacterium aerofaciens, clostridium perfringens, escherichia coli, and lactobacillus acidophilus were examined. adhesive rate of lkm512 to the mucin was significantly (p < 0.05, 0.01, or 0.001) stronger than the other strains from 2 to 100 time. though the adhesive property of many strains was almost same to ...200211821930
accelerated alveolar bone loss in hla-b27 transgenic rats: an adult onset condition.patients with arthritis and crohn's disease may be more susceptible to periodontitis associated alveolar bone loss (abl). hla-b27 transgenic (tg) rats spontaneously develop arthritis and colitis. based on the hypothesis that tg rats would also be susceptible to abl, we compared the naturally occurring abl in tg and fischer 344 wild-type (wt) rats.200212064843
an oral introduction of intestinal bacteria prevents the development of a long-term th2-skewed immunological memory induced by neonatal antibiotic treatment in mice.recent epidemiological studies indicate that antibiotic use in infancy may be associated with an increased risk of developing atopy. our previous work on animals demonstrated that kanamycin use during infancy promotes a shift in the th1/th2 balance towards a th2-dominant immunity.200212100062
structural study on lipid a and the o-specific polysaccharide of the lipopolysaccharide from a clinical isolate of bacteroides vulgatus from a patient with crohn's disease.bacteroides vulgatus has been shown to be involved in the aggravation of colitis. previously, we separated two potent virulence factors, capsular polysaccharide (cps) and lipopolysaccharide (lps), from a clinical isolate of b. vulgatus and characterized the structure of cps. in this study, we elucidated the structures of o-antigen polysaccharide (ops) and lipid a in the lps. lps was subjected to weak acid hydrolysis to produce the lipid a fraction and polysaccharide fraction. lipid a was isolate ...200212153568
quantitative analysis of bacterial dna from mycobacteria spp., bacteroides vulgatus, and escherichia coli in tissue samples from patients with inflammatory bowel diseases.the etiology of inflammatory bowel diseases is unknown. mycobacteria spp., bacteroides vulgatus, and escherichia coli have been suspected to be involved. the aim of the present study was to examine the possible relationship between inflammatory bowel diseases and these microbes.200212162408
design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples.an oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ...200212167534
innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora.the hygiene hypothesis postulates that the prevalence of allergy has increased due to decreased microbial stimulation early in life, leading to delayed maturation of the immune system. the aim of this study was to examine the cytokine pattern produced from cord blood mononuclear cells relative to adult cells after stimulation with bacterial strains from the normal flora. mononuclear cells from cord and adult blood samples were stimulated with the following bacteria: bifidobacterium adolescentis, ...200212438343
quantification of bacteria adherent to gastrointestinal mucosa by real-time pcr.the use of real-time quantitative pcr (5' nuclease pcr assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. we developed primers and probes based on the 16s ribosomal dna gene sequences for the detection of escherichia coli and bacteroides vulgatus. dna was isolated from pure cultures and from gut biopsy specimens and quantified by the 5' nuclease pcr assay. the assay showed a very high sensitivity: as little as 1 cfu of e. coli and 9 cfu of ...200212454130
in vitro activities of newer quinolones against bacteroides group organisms.the activities of bms-284576, clinafloxacin, moxifloxacin, sitafloxacin, trovafloxacin, imipenem, cefoxitin, and clindamycin against 589 bacteroides fragilis group isolates were determined. the activity of bms-284576 was comparable to that of trovafloxacin. sitafloxacin and clinafloxacin were the most active quinolones, and moxifloxacin was the least active. b. fragilis was the most susceptible of the species, and bacteroides vulgatus was the most resistant. association of specific antibiotic re ...200212234859
national survey on the susceptibility of bacteroides fragilis group: report and analysis of trends for 1997-2000.the results of a multicenter us survey using the national committee for clinical laboratory standards currently recommended methodology for measuring in vitro susceptibility of 2673 isolates of bacteroides fragilis group species were compared from 1997 to 2000. the test panel consisted of 14 antibiotics: 3 carbapenems, 3 beta-lactam-beta-lactamase inhibitors, 3 cephamycins, 2 fluoroquinolones, clindamycin, chloramphenicol, and metronidazole. declines in the geometric mean minimum inhibitory conc ...200212173121
bats and their control. 2002133091
[instrumental perforations of the esophagus]. 2002139654
[sero-epidemiological surveillance, a method of prevention of complications of streptococcal infections].in order to find methods for the prevention and control of streptococcal infections of 711 day schoolchildren and boarders, aged 7 to 14 years, were followed up during the 1969-1973 period. the clinical and epidemiologic investigations revealed a great number of acute infections with varied clinical manifestations and high proportions of the acute articular rheumatism (3.1%) in the boarding schools as compared to the day schools (0.5%). the bacteriologic examinations confirmed and established th ...2002128801
the surgical management of low back and sciatic syndrome in disc disease or injury : results of a joint neurosurgical and orthopaedic project. 2002127575
cricopharyngeal sphincterotomy and bilateral division of the chorda tympani in bulbar palsy. 2002128757
the diagnosis of genitourinary neoplasms. 2002123911
clinical, electroencephalographic and brain scan correlation in focal epilepsy: (a retrospective study in 97 patients). 2002120089
[detection of horsefly larvae in winter far from the water reservoirs]. 2002139536
myocardial blood flow in congestive and hypertrophic cardiomyopathy: relationship to peak wall stress and mean velocity of circumferential fiber shortening.myocardial blood flow/unit mass (mbf) and the determinants of myocardial oxygen consumption were measured in seven control subjects (group i) and 15 patients (pts) with cardiomyopathy (cm), group ii (group iia-congestive cm: 10 pts; group iib-hypertrophic cm: 5 pts). in group i left ventricular (lv) mbf was 64 +/- 8 (sd) ml/100g-min; it was significantly lower in iia (45 +/- 15 ml/100g-min, p less than 0.01) and iib (39 +/- 7 ml/100g-min, p less than 0.01). however, calculated total lv flow (lv ...2002133002
role of commensal bacteria in chronic experimental colitis: lessons from the hla-b27 transgenic rat.rats on lewis or fischer background, transgenic for human hla-b27 and beta(2)-microglobulin genes spontaneously develop colitis, gastritis, arthritis, dermatitis, orchitis, epididymitis, carditis, alopecia and nail changes. disease susceptibility correlates with the gene copy number and is influenced by the genetic background. the pathomechanism in this model is still not completely understood. cell transfer experiments indicate an essential role of hla-b27 expression in bone marrow-derived cell ...200212571416
psychiatrists with blinkers. 200187973
long-term survival from acute myocardial infarction: salutary effect of an open coronary vessel. 20012195885
chinese hamster purine nucleoside phosphorylase. 200199640
comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria.the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ...200111120995
multicenter survey of the changing in vitro antimicrobial susceptibilities of clinical isolates of bacteroides fragilis group, prevotella, fusobacterium, porphyromonas, and peptostreptococcus species.in vitro surveys of antimicrobial resistance among clinically important anaerobes are an important source of information that can be used for clinical decisions in the choice of empiric antimicrobial therapy. this study surveyed the susceptibilities of 556 clinical anaerobic isolates from four large medical centers using a broth microdilution method. piperacillin-tazobactam was the only antimicrobial agent to which all the isolates were susceptible. similarly, imipenem, meropenem, and metronidaz ...200111257040
gyra mutations associated with quinolone resistance in bacteroides fragilis group strains.mutations in the gyra gene contribute considerably to quinolone resistance in escherichia coli. mechanisms for quinolone resistance in anaerobic bacteria are less well studied. the bacteroides fragilis group are the anaerobic organisms most frequently isolated from patients with bacteremia and intraabdominal infections. forty-four clinafloxacin-resistant and-susceptible fecal and clinical isolates of the b. fragilis group (eight bacteroides fragilis, three bacteroides ovatus, five bacteroides th ...200111408211
cloning and biochemical characterization of a class a beta-lactamase from prevotella intermedia.the gene encoding a beta-lactamase of prevotella intermedia was cloned and sequenced. this gene, called cfxa2, shared 98% identity with cfxa, the structural gene of a beta-lactamase previously described in bacteroides vulgatus. the deduced protein sequence had a k272e substitution. cfxa2 had the characteristics of class a, group 2e beta-lactamases.200111451705
sequence analysis of the plasmid prri2 from the rumen bacterium prevotella ruminicola 223/m2/7 and the use of prri2 in prevotella/bacteroides shuttle vectors.prri2 is a small cryptic plasmid from the rumen bacterium prevotella ruminicola 223/m2/7 which has been used for the construction of shuttle vectors (prh3 and prri207) that replicate in many bacteroides/prevotella strains as well as in escherichia coli. sequence analysis of prri2 reveals that it is a 3240-bp plasmid carrying two clear open reading frames. rep, encoded by orf1, shows 48 and 47% amino acid sequence identity with repa proteins from bacteroides vulgatus and bacteroides fragilis, res ...200111407918
structural elucidation of a capsular polysaccharide from a clinical isolate of bacteroides vulgatus from a patient with crohn's disease.the structure of a capsular polysaccharide (cps) from a clinical isolate of bacteroides vulgatus was elucidated. b. vulgatus imcj 1204 was isolated from feces of a patient with crohn's disease. cps was prepared by phenol/water extraction of the bacterial cells followed by hydrophobic interaction chromatography and then gel filtration chromatography of the extract. the structure of cps was determined by chemical analysis and nmr spectroscopy including dqf-cosy, tocsy, roesy, hsqc-tocsy, hmqc and ...200111389714
brain metastasis: current status and recommended guidelines for management.an overview of brain metastasis with respect to the pathological, diagnostic, and therapeutic aspects is presented. management is almost always palliative, with cure being a rare exception. evaluation of various therapeutic modalities--radiation, chemotherapy, or surgery--has been confounded by a lack of controlled, randomized studies whereby the relative benefit of the respective modalities can be assessed objectively. despite these limitations, some progress is being made in the identification ...200193255
[symposium on major histocompatibility antigens (hla) and diseases. (3) hla types in rheumatic disease (author's transl)]. 200193137
post basic education. 200198156
mucin-producing carcinomas of the breast: ultrastructural observations.the ultrastructural characteristics of mucin production in mammary mucoid carcinoma, signet ring cell carcinoma (harris, wells & vasudev 1978), papillary carcinoma and lobular carcinoma are compared. the mucin in lobular carcinoma is confined to intracytoplasmic lumina whereas it is present as membrane bound granules in the other three types, although intracytoplasmic lumina also occur in the latter. a possible origin of intracytoplasmic lumina from distended golgi cisternae is proposed. possibl ...2001208954
organizing library audiovisual services to support continuing education. 200190336
fetal heart-rate monitoring. 200190290
[behavior of some acute phase reactant proteins during phlogosis and neoplasms and their correlation with erythrocyte sedimentation rate].alpha 2 macroglobulin, haptoglobin and fibrinogen plasma levels were simultaneously determined, as well as e.s.r., to test if a significant difference could be found among cancer, inflammatory diseases and control subjects. haptoglobin was higher in the cancer group and fibrinogen in inflammatory diseases, but these selective increases are not believed to be enough to suggest the widespread determinations of these proteins. a significant correlation was found between alpha 2 macroglobulin and e. ...200195158
immediate results of weekly fractionation in external radiotherapy. 2000101487
design, devices, and discipline in operating room infection control.the prevention of surgical infection in the operating theatre is a complex pursuit. every facet of activity, whether it is part of the surgical act itself or a remote activity with indirect effects on the surgical operation, constitutes part of the whole realm of infection control. these many facets may be divided into four main components, all interdependent: (a) the surgeon and his team (surgical technique; adherence to surgical anatomic, physiologic, and aseptic principles; discipline); (b) e ...2000101749
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