Publications

comparative functional genomic analysis of pasteurellaceae adhesins using phage display.	the pasteurellaceae contain a number of important animal pathogens. although related, the various members of this family cause a diversity of pathology in a wide variety of organ systems. adhesion is an important virulence factor in bacterial infections. surprisingly little is known about the adhesins of the pasteurellaceae. to attempt to identify the genes coding for adhesins to some key components of the hosts extracellular matrix molecules, phage display libraries of fragmented genomic dna fr ...	2007	17258409
cpg oligodeoxynucleotides augment the immune responses of piglets to swine pasteurella multocida living vaccine in vivo.	oligodeoxynucleotides containing unmethylated cpg motifs (cpg odn) prevent development of t-helper type 2 (th2) immune response and reverse established allergic responses in mouse models. however, little work on immune responses in piglets has been conducted in vivo. in this report, the ability of a porcine-specific cpg odn to act as an immunostimulant and enhance immune responses of piglets to swine pasteurella multocida living vaccine (spml vaccine) was determined. the titre of igg and igg1/ig ...	2007	17223146
pasteurella multocida urinary tract infection in a pediatric patient with end-stage renal disease.	pasteurella multocida is a gram-negative bacillus that is part of the normal oral flora of cats and dogs. most infections involving p. multocida are soft tissue infections after animal bites or scratches. we present a case of p. multocida urinary tract infection in a 13-year-old boy with end-stage renal disease receiving peritoneal dialysis. he was successfully treated with intravenous ampicillin-sulbactam followed by oral amoxicillin-clavulanate. thirteen additional cases of p. multocida urinar ...	2007	17259887
using capacitance measurements as the detection method in antigen-containing layer-by-layer films for biosensing.	the layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. detection was carried out using a novel strategy entirely based upon capacitance measurements, and to enhance sensitivity, we combine the response of three different sensing units in a similar procedure used for taste sensors. with the three-electrode array immunoglobul ...	2007	17263514
differentiation of avian pasteurella multocida strains by single-primer pcr.		2007	17294263
isolated endocarditis of the pulmonary valve caused by pasteurella multocida.	we describe the rare case of a patient with an isolated endocarditis of the pulmonary valve caused by pasteurella multocida. the bacterium was cultured from blood as well as from the excised valve after pulmonary valve replacement. risk factors were contact with animals and concurrent intravenous drug use.	2007	17297591
new consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria.	the clinical and laboratory standards institute (clsi) recently published a new laboratory guideline for antimicrobial susceptibility testing of infrequently encountered or fastidious bacteria not covered in previous clsi publications. the organisms include aeromonas species, bacillus species, and vibrio species that may cause infections following environmental exposure. fastidious organisms that may cause endocarditis or medical device infections include abiotrophia and granulicatella species; ...	2007	17173232
apoptosis in malignant glioma cells triggered by the temozolomide-induced dna lesion o6-methylguanine.	methylating drugs such as temozolomide (tmz) are widely used in the treatment of brain tumours (malignant gliomas). the mechanism of tmz-induced glioma cell death is unknown. here, we show that malignant glioma cells undergo apoptosis following treatment with the methylating agents n-methyl-n'-nitro-n-nitrosoguanidine (mnng) and tmz. cell death determined by colony formation and apoptosis following methylation is greatly stimulated by p53. transfection experiments with o(6)-methylguanine-dna met ...	2007	16819506
etiology of respiratory disease in non-vaccinated, non-medicated calves in rearing herds.	the aim of this study was to examine the occurrence of bacterial, mycoplasmal and viral pathogens in the lower respiratory tract of calves in all-in all-out calf-rearing units. according to clinical status, non-medicated calves with and without respiratory disease signs were selected of the 40 herds investigated to analyse the micro-organisms present in healthy and diseased calves. tracheobronchial lavage (tbl) and paired serum samples were analysed for bacteria, mycoplasmas, respiratory syncyti ...	2007	17084565
identification of novel immunogens in pasteurella multocida.	p. multocida is a gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. current vaccines include bacterins, which provide only limited protection against homologous serotypes. therefore there is a need for more effective vaccines to control diseases caused by p. multocida. as a step towards developing vaccines against fowl cholera, a genomics based approach was applied for the identification of novel immunogens. ...	2007	17233917
modulation of host cell gene expression through activation of stat transcription factors by pasteurella multocida toxin.	the pasteurella multocida toxin (pmt) is highly mitogenic and has potential carcinogenic properties. pmt causes porcine atrophic rhinitis that is characterized by bone resorption and loss of nasal turbinates, but experimental nasal infection also leads to excess proliferation of bladder epithelial cells. pmt acts intracellularly and activates phospholipase c-linked signals and mapk pathways via the heterotrimeric galpha(q) and galpha(12/13) proteins. we found that pmt induces activation of stat ...	2007	17150962
the functional molecular mass of the pasteurella hyaluronan synthase is a monomer.	hyaluronan (ha), a linear polysaccharide composed of beta1,3-glcnac-beta1,4-glcua repeats, is found in the extracellular matrix of vertebrate tissues as well as the capsule of several pathogenic bacteria. all known ha synthases (hass) are dual-action glycosyltransferases that catalyze the addition of two different sugars from udp-linked precursors to the growing ha chain. the bacterial hyaluronan synthase, pmhas from gram-negative pasteurella multocida, is a 972-residue membrane-associated prote ...	2007	17095162
full genome sequence and some biological properties of reticuloendotheliosis virus strain apc-566 isolated from endangered attwater's prairie chickens.	reticuloendotheliosis virus (rev) causes runting, high mortality, immunosuppression, and chronic neoplasia associated with t and/or b cell lymphomas in a variety of domestic and wild birds, including attwater's prairie chickens (apc) (tympanuchus cupido attwateri). the complete proviral sequence of a recent rev isolate from apc (rev apc-566) was determined. this virus was isolated from an apc maintained in captivity in a reproduction program intended to avoid its extinction. rev apc-566 was dete ...	2007	17098316
efficacy of haemorrhagic septicaemia alum-precipitated vaccine.		2007	17277309
acceptor specificity of the pasteurella hyaluronan and chondroitin synthases and production of chimeric glycosaminoglycans.	the hyaluronan (ha) synthase, pmhas, and the chondroitin synthase, pmcs, from the gram-negative bacterium pasteurella multocida polymerize the glycosaminoglycan (gag) sugar chains ha or chondroitin, respectively. the recombinant escherichia coli-derived enzymes were shown previously to elongate exogenously supplied oligosaccharides of their cognate gag (e.g. ha elongated by pmhas). here we show that oligosaccharides and polysaccharides of certain noncognate gags (including sulfated and iduronic ...	2007	17099217
cloning of the gene and characterization of the enzymatic properties of the monomeric alkaline phosphatase (phox) from pasteurella multocida strain x-73.	we have identified a new phox gene encoding the monomeric alkaline phosphatase from pasteurella multocida x-73. this gene was not found in the published genome sequence of pasteurella multocida pm70. characterization of the recombinant phox of pasteurella multocida x-73 showed that it is a monomeric enzyme, activated by ca(2+) and possibly secreted by the tat pathway. these features distinguish phosphatases of the phox family from those of the phoa family. all proteins of the phox family were fo ...	2007	17156125
quantitative continuous assay for hyaluronan synthase.	a rapid, continuous, and convenient three-enzyme coupled uv absorption assay was developed to quantitate the glucuronic acid and n-acetylglucosamine transferase activities of hyaluronan synthase from pasteurella multocida (pmhas). activity was measured by coupling the udp produced from the pmhas-catalyzed transfer of udp-glcnac and udp-glcua to a hyaluronic acid tetrasaccharide primer with the oxidation of nadh. using a fluorescently labeled primer, the products were characterized by gel electro ...	2007	17173853
[pneumonia with septicemia caused by pasteurella multocida in an immunocompetent patient].	pneumonia with septicemia caused by pasteurella multocida was diagnosed in an immunocompetent patient exposed to a dog. this case is remarkable by two aspects: first the absence of visible cutaneous lesion, and second the localization and severity of the infection caused by p. multocida even though the patient was immunocompetent. p. multocida can cause respiratory and systemic infection, and it is a possible diagnosis in case of exposure to animals, even without history of bite or scratch. furt ...	2007	17303362
cloning and characterization of type 4 fimbrial gene (ptfa) of pasteurella multocida serogroup b:2 (strain p52).		2007	17216317
phylogenetic diversity of pasteurellaceae and horizontal gene transfer of leukotoxin in wild and domestic sheep.	wild and domestic animal populations are known to be sources and reservoirs of emerging diseases. there is also a growing recognition that horizontal genetic transfer (hgt) plays an important role in bacterial pathogenesis. we used molecular phylogenetic methods to assess diversity and cross-transmission rates of pasteurellaceae bacteria in populations of bighorn sheep, dall's sheep, domestic sheep and domestic goats. members of the pasteurellaceae cause an array of deadly illnesses including ba ...	2007	16635591
sinonasal plasmacytoma in a cat.	a 13-year-old female spayed domestic shorthair cat presented with a history of right-sided mucopurulent nasal discharge for 18 months. computed tomography revealed a mass within the right nasal cavity and the right frontal sinus. the animal was euthanized, and a postmortem examination was performed. on macroscopic examination, the right nasal cavity and the right frontal sinus were partially occluded by a soft whitish mass. microscopically, the mass was composed of well-differentiated plasma cel ...	2007	17823407
pathogenic and opportunistic gram-negative bacteria in soil, leachate and air in san nicolás landfill at aguascalientes, mexico.	the occurrence of gram-negative pathogenic and opportunistic species, was studied for two years on air. soil and leachate from the san nicolás landfill. aguascalientes, mexico. for soil and leachate four samplings were done, two during the dry season and two during the rainy season. for soil there were 15 sampling points, the leachate samples were taken on the leachate tank. for air, twelve sampling were done in three points of the landfill. twenty pathogenic and/or opportunistic bacteria were i ...	2007	18693549
cardiovascular device infections in dogs: report of 8 cases and review of the literature.	infection is an infrequent but major complication of cardiovascular device implantation.	2007	18196736
pasteurella multocida sepsis and meningitis in 2-month-old twin infants after household exposure to a slaughtered sheep.	this article outlines 2 cases of pasteurella multocida sepsis and meningitis in 2-month-old twins after their father was exposed to a sheep during a sacrifice celebration at home. these cases emphasize the necessity of respecting basic hygiene rules and the danger involved in animal sacrifice without suitable professional structure.	2007	17712748
quantitative resistance level (mic) of pasteurella multocida isolated from pigs between 2004 and 2006: national resistance monitoring by the bvl.	the national resistance monitoring of the federal office of consumer protection and food safety (bvl) is to determine the prevalence of resistance of bacterial pathogens from animals using a valid database. from 2004 to 2006, a total of 1,472 pasteurella multocida strains isolated from pigs with acute respiratory tract diseases was submitted to the bvl and examined. of these, 1,11 (75.5 %) were included in the study and tested using 24 different antimicrobial substances. the results showed that ...	2007	17939459
[pasteurella multocida pneumonia with molecular evidence of zoonotic transmission].	we report the case of a patient with pneumonia caused by pasteurella multocida (p. multocida). an 83-year-old woman admitted for bronchiectasis, productive cough, and a high fever was found in chest radiography on admission to have a new infiltrative shadow in the left lower lung field. chest computed tomography indicated bilateral bronchiectasis and an infiltrative lingular shadow. p. multocida was isolated from sputum samples. biapenem and minocycline therapy ameliorated symptoms. pulsed-field ...	2007	18095473
pasteurella multocida infections.		2007	18055649
microorganisms associated with a pneumonic epizootic in rocky mountain bighorn sheep (ovis canadensis canadensis).	a comprehensive study of a pneumonic epizootic was initiated when the first signs of disease were noted in a metapopulation of bighorn sheep inhabiting hells canyon, bordering idaho, oregon, and washington. a total of 92 bighorn sheep were tested for etiologic agents during the following 6-mo study period. the study population included bighorn sheep believed to be the subpopulation in which disease was first noted, and these sheep were translocated to a holding facility in an effort to contain t ...	2007	18229860
evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay.	serum amyloid a (saa) is of interest as the circulating precursor of amyloid a protein, the fibrillar component of aa (secondary) amyloid deposits, and also as an extremely sensitive and rapid major acute phase protein. serum concentrations of acute phase proteins (apps) provide valuable information about the diagnosis and prognosis of various diseases, and thus the relevance of apps for monitoring the health status of domestic animals is widely accepted. more importantly, the measurement of saa ...	2007	17867462
dna fingerprinting analysis of breakthrough outbreaks in vaccine-protected poultry stocks.	we report recurrent outbreaks of yersinia pseudotuberculosis conjunctivitis in ducks and of fowl cholera in geese, occurring in stocks previously vaccinated with inactivated autogenous vaccines. enterobacterial repetitive intergenic consensus sequence-based pcr and pulsed-field gel electrophoresis indicated reinfection with a new y. pseudotuberculosis strain and vaccine evasion by the same pasteurella multocida strain.	2007	17928428
biochemical characterization of a neisseria meningitidis polysialyltransferase reveals novel functional motifs in bacterial sialyltransferases.	the extracellular polysaccharide capsule is an essential virulence factor of neisseria meningitidis, a leading cause of severe bacterial meningitis and sepsis. serogroup b strains, the primary disease causing isolates in europe and america, are encapsulated in alpha-2,8 polysialic acid (polysia). the capsular polymer is synthesized from activated sialic acid by action of a membrane-associated polysialyltransferase (nmb-polyst). here we present a comprehensive characterization of nmb-polyst. diff ...	2007	17662040
purification, crystallization and preliminary crystallographic characterization of the alpha 2,6-sialyltransferase from photobacterium sp. jt-ish-224.	sialyltransferases transfer sialic acid from cytidine-5-monophospho-n-acetylneuraminic acid (cmp-neuac) to the nonreducing termini of the oligosaccharyl structures of various glycoproteins and glycolipids. the newly cloned alpha2,6-sialyltransferase from photobacterium sp. jt-ish-224 (from the vibrionaceae family) is composed of two domains: an unknown n-terminal domain and a catalytic c-terminal domain which shares significant homology with the pasteurella multocida multifunctional sialyltransf ...	2007	17671362
[severe pasteurella multocida pneumonia by close contact with a pet in chronic respiratory failure].		2007	17682434
pasteurella multocida septic shock following liver transplantation treated with drotrecogin alpha (activated).	severe sepsis and progression to septic shock in solid organ transplant recipients is associated with a high mortality. we describe a fulminant case of septic shock in a liver transplant recipient caused by pasteurella multocida, a gram-negative coccobacillus most commonly associated with domestic cats and dogs. p. multocida is a rare cause of bacteremia and has not been reported as a cause of septic shock following liver transplantation. in addition to standard therapy, the patient was managed ...	2007	17692071
reversible myocardial depression associated with sepsis in a dog.		2007	17939574
decoration of pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence.	phosphocholine (pcho) is an important substituent of surface structures expressed by a number of bacterial pathogens. its role in virulence has been investigated in several species, in which it has been shown to play a role in bacterial adhesion to mucosal surfaces, in resistance to antimicrobial peptides, or in sensitivity to complement-mediated killing. the lipopolysaccharide (lps) structure of pasteurella multocida strain pm70, whose genome sequence is known, has recently been determined and ...	2007	17704225
recombinant synthesis of hyaluronan by agrobacterium sp.	hyaluronan (ha) is a sugar polymer of a repeating disaccharide, beta1-3 d-n-acetylglucosamine (glcnac) beta1-4 d-glucuronic acid (glca). it finds applications in numerous biomedical procedures such as ophthalmic surgery and osteoarthritis treatment. until recently, the only commercial sources were extraction of rooster combs and from fermentation of pathogenic streptococcus. in this work, we demonstrate that metabolic engineering strategies enable the recombinant synthesis of hyaluronan in a saf ...	2007	17705506
pasteurella multocida bacteremia in asymptomatic plateletpheresis donors: a tale of two cats.	bacterial contamination of platelet (plt) concentrates occurs in 1 in 1000 to 1 in 3000 components and has been a leading cause of transfusion-associated morbidity and mortality. two cases of pasteurella multocida bacteremia in asymptomatic plateletpheresis donors are reported. clinical outcomes were profoundly different, emphasizing the importance of robust methods to detect bacterial contamination.	2007	17958526
immunological roles of pasteurella multocida toxin (pmt) using a pmt mutant strain.	the immunological role of the pasteurella multocida toxin (pmt) in mice was examined using a pmt mutant strain. after a nasal inoculation, the mutant strain failed to induce interstitial pneumonia. moreover, pmt had no significant effect on the populations of cd4+, cd8+, cd3+, and cd19+ immunocytes in blood or on the populations of cd4+ and cd8+ splenocytes (p<0.01). however, there was a significant increase in the total number of cells in the bal samples obtained from the wild-type p. multocida ...	2007	17846592
safety and protective efficacy of intramuscular vaccination with a live aroa derivative of pasteurella multocida b:2 against experimental hemorrhagic septicemia in calves.	three groups of five calves, namely, v1, v2, and v3, were immunized intramuscularly at 4 and 8 weeks of age with ca. 10(9), 10(8), and 10(7) cfu, respectively, of a derivative of pasteurella multocida b:2 wild-type strain 85020 containing a deletion in the aroa gene (strain jrmt12). the first and second vaccinations resulted in significantly (p < 0.01) higher rectal temperature responses in groups v1 and v2 than in group v3. serum immunoglobulin m (igm) and igg titers did not increase in any gro ...	2007	17875632
a multidomain outer membrane protein from pasteurella multocida: modelling and simulation studies of pmompa.	pmompa is a two-domain outer membrane protein from pasteurella multocida. the n-terminal domain of pmompa is a homologue of the transmembrane beta-barrel domain of ompa from escherichia coli, whilst the c-terminal domain of pmompa is a homologue of the extra-membrane neisseria meningitidis rmpm c-terminal domain. this enables a model of a complete two domain pmompa to be constructed and its conformational dynamics explored via md simulations of the protein embedded within two different phospholi ...	2007	17888868
susceptibility of bacterial pathogens against lincomycin/spectinomycin (1/2), penicillin g/neomycin (1/1), and penicillin g/dihydrostreptomycin (1/1) as determined in the bft-germvet monitoring program 2004-2006.	a total of 368 bacterial pathogens, including 72 coagulase-positive and coagulase-variable staphylococci, 97 beta-haemolytic streptococci, 51 escherichia coli, 75 pasteurella multocida, 25 mannheimia haemolytica, 25 pseudomonas aeruginosa, and 23 arcanobacterium pyogenes, were investigated for their susceptibility to the three combinations of antimicrobial agents lincomycin/spectinomycin (1/2), penicillin g/neomycin (1/1), and penicillin g/dihydrostreptomycin (1/1) in comparison to their suscept ...	2007	17939451
pasteurella multocida and bovine respiratory disease.	pasteurella multocida is a pathogenic gram-negative bacterium that has been classified into three subspecies, five capsular serogroups and 16 serotypes. p. multocida serogroup a isolates are bovine nasopharyngeal commensals, bovine pathogens and common isolates from bovine respiratory disease (brd), both enzootic calf pneumonia of young dairy calves and shipping fever of weaned, stressed beef cattle. p. multocida a:3 is the most common serotype isolated from brd, and these isolates have limited ...	2007	18218157
[acclimating, screening of dominant bacteria for degrading phthalic acid esters and study on its degradation].	to define the method of acclimating isolating and screening of dominant bacteria for phthalic acid esters biodegradation and study their plasmids.	2007	18303624
antimicrobial susceptibility of pasteurella multocida and bordetella bronchiseptica from dogs and cats as determined in the bft-germvet monitoring program 2004-2006.	a total of 92 canine/feline pasteurella multocida strains form respiratory tract infections or infections of skin/ear/mouth as well as 42 canine/feline bordetella bronchiseptica strains from respiratory tract infections were investigated for their susceptibility to antimicrobial agents. while the p. multocida strains were susceptible to all antimicrobial agents tested - except sulfonamides -, a considerable number of the b. bronchiseptica strains was resistant or exhibited high mic values agains ...	2007	17939457
phenotypic and genotypic characterisation of pasteurella multocida strains isolated from pigs in hungary.	a total of 146 pasteurella multocida strains isolated from swine in hungary in the last 20 years were examined. biochemical characterisation and pcr-based techniques were used to determine species, subspecies, biovar, capsule type and presence of the toxa gene. eighty-seven percent of the isolates belonged to p. multocida ssp. multocida, and 98% of these had biovar 3 or were trehalose- or lactose-fermenting or ornithine decarboxylase negative variants of that. ten percent of the strains were p. ...	2007	18277702
[frequency of occurrence of pneumonia associated agents and their correlation with clinical and pathological-anatomical findings in pigs].	the aim of this study was to investigate the incidence and influence of different causative organisms involved in the development of pneumonia and bronchopneumonia in pigs. bronchoalveolar lavage fluid (balf) from 339 pigs was examined molecular-biologically and culturally. the evaluation considered the following pathogens: mycoplasma (m.) hyopneumoniae, mycoplasma (m.) hyorhinis, prrsv (us-type), prrsv (eu-type), pcv-2, influenzavirus type a, alpha-haemolytic streptococci, beta-haemolytic strep ...	2007	18085159
immune evasion by pathogens of bovine respiratory disease complex.	bovine respiratory tract disease is a multi-factorial disease complex involving several viruses and bacteria. viruses that play prominent roles in causing the bovine respiratory disease complex include bovine herpesvirus-1, bovine respiratory syncytial virus, bovine viral diarrhea virus and parinfluenza-3 virus. bacteria that play prominent roles in this disease complex are mannheimia haemolytica and mycoplasma bovis. other bacteria that infect the bovine respiratory tract of cattle are histophi ...	2007	18218162
effect of centchroman on cellular and humoral immunity.	centchroman (ormeloxifene) is a nonsteroidal selective estrogen receptor modulator that is used as once a week oral contraceptive agent. the effect of centchroman on the immune system was evaluated by using different experimental models such as carbon clearance test, cyclophosphamide induced neutropenia, neutrophil adhesion test, effect on serum immunoglobulins, mice lethality test and indirect haemagglutination test. the first three models namely carbon clearance test, cyclophosphamide induced ...	2007	18476393
accuracy of susceptibility testing of pasteurella multocida and mannheimia haemolytica.	the accuracy of antimicrobial susceptibility determinations relies on the bacterial species identification and the test methodology that is being used. in the present study, both aspects were investigated for 141 pasteurella multocida and 34 mannheimia haemolytica sensu lato isolates recovered from the upper respiratory tract of healthy calves. this was performed on the one hand by comparing of classical phenotyping with tdna-pcr genotyping and on the other hand by pairwise comparison of disk di ...	2007	17949308
quantitative resistance level (mic) of bacterial pathogens (escherichia coli, pasteurella multocida, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus) isolated from chickens and turkeys: national resistance monitoring by the bvl 2004/2005.	in the study 2004/2005, the current quantitative resistance level of escherichia coli, pasteurella multocida, pseudomonas aeruginosa, salmonella sp. and staphylococcus aureus from chickens and turkeys was determined for the first time within the framework of the national resistance monitoring of the federal office of consumer protection and food safety (bvl). the objective was to implement a valid database on the basis of which the development and spread of resistance can be evaluated and monito ...	2007	17939460
pharmacological evidence for activation of phospholipid and small gtp binding protein signalling cascades by nod factors.	the effects of lipo-chitin oligosaccharide nod factors (nodngr[s] from rhizobium sp. ngr234) on root hair deformation in vigna unguiculata (l.) walp. were studied using pharmacological agents to mimic and/or inhibit their action. it was hypothesised that the rearrangement of the cytoskeleton seen during nod factor induced root hair deformation is modulated by protein kinase c, monomeric g proteins of the rho superfamily and the location and amount of phosphatidylinositol 3-phosphates (pi3ps). th ...	2006	16647267
dental follicle infection following a dog bite.	animal bite wounds and their subsequent infection are relatively common. incidence rates for dog bites are significantly higher among children aged 0-9 years, especially among boys. although bite wounds may initially look innocuous, they frequently lead to serious infection with a potential for life-threatening complications. the microbiology of dog bite wounds is usually polymicrobial, typically including anaerobes, staphylococcus aureus and pasteurella species. a case is described of a 22-mont ...	2006	16430531
monitoring of antimicrobial resistance in pathogenic bacteria from livestock animals.	facing the problem of development and spreading of bacterial resistance, preventive strategies are considered the most appropriate means to counteract. the establishment of corresponding management options relies on scientifically defensible efforts to obtain objective data on the prevalence of bacterial resistance in healthy and diseased livestock. additionally, detailed statistics are needed on the overall amount of antimicrobial agents dispensed in germany. the collection of valid data on the ...	2006	16530479
persistence of pasteurella multocida in wetlands following avian cholera outbreaks.	avian cholera, caused by pasteurella multocida, affects waterbirds across north america and occurs worldwide among various avian species. once an epizootic begins, contamination of the wetland environment likely facilitates the transmission of p. multocida to susceptible birds. to evaluate the ability of p. multocida serotype-1, the most common serotype associated with avian cholera in waterfowl in western and central north america, to persist in wetlands and to identify environmental factors as ...	2006	16699146
lung lesions in scid-bo and scid-bg mice after intratracheal inoculation with wild-type or leucotoxin-deficient mutant strains of mannheimia haemolytica serotype 1.	the purpose of this study was to investigate scid-bg mice engrafted with bovine haematolymphoid tissues (scid-bo) as a model for studying bovine mannheimia haemolytica serotype 1- induced pneumonia, in which leucotoxin (lkt) plays a major role. in experiment a, scid-bo and scid-bg mice were inoculated intratracheally with either (1) phosphate-buffered saline (pbs), (2) m. haemolytica wild-type strain 89010807n ("lkt(+)wt"), (3) a m. haemolytica leucotoxin-deficient mutant of strain 89010807n ("l ...	2006	16712865
[pasteurella multocida meningitis following neurosurgery].	pasteurella multocida is a rare cause of meningitis. we report a case of p. multocida meningitis following a recent neurosurgery procedure and review the 32 cases previously described.	2006	16630702
analytical verification of a multiplex pcr for identification of bordetella bronchiseptica and pasteurella multocida from swine.	bordetella bronchiseptica and pasteurella multocida are etiologic agents of progressive atrophic rhinitis (par) and bronchopneumonia in swine. only dermonecrotic toxin-producing strains of p. multocida play a role in atrophic rhinitis while both toxigenic and nontoxigenic strains have been associated with pneumonia. monitoring and investigation of outbreaks involving these bacteria require sensitive and accurate identification and reliable determination of the toxigenic status of p. multocida is ...	2006	16782287
complications of a cat bite.		2006	16988179
antimicrobial susceptibility patterns of respiratory tract pathogens from sheep and goats.	to determine the antimicrobial susceptibility of common respiratory tract pathogens from sheep and goats.	2006	17042733
pasteurella multocida pathogenesis: 125 years after pasteur.	pasteurella multocida was first shown to be the causative agent of fowl cholera by louis pasteur in 1881. since then, this gram-negative bacterium has been identified as the causative agent of many other economically important diseases in a wide range of hosts. the mechanisms by which these bacteria can invade the mucosa, evade innate immunity and cause systemic disease are slowly being elucidated. key virulence factors identified to date include capsule and lipopolysaccharide. the capsule is cl ...	2006	17107417
potential pathogens carried by spanish ibex (capra pyrenaica hispanica) in southern spain.	the spanish ibex (capra pyrenaica hispanica) population of southern spain was surveyed for potential pathogens associated with the conjunctiva, external ear canal, as well as reproductive and upper respiratory tracts. we sampled 321 ibex (131 adult males, 100 adult females, and 90 yearlings); these included 271 apparently healthy animals and 50 that were naturally infected with sarcoptes scabiei. a total of 688 bacterial isolates were identified (377 gram-negatives, 225 gram-positives, and 86 my ...	2006	16870855
phylogenetic analysis of isolates of pasteurella pneumotropica from laboratory animals based on the gyrb gene sequence.	phylogenetic analysis using the gyrb sequence was performed to investigate the genetic relevance among 49 isolates of p. pneumotropica. in the phylogeny, the isolates were clearly classified into three groups as follows: group a for the isolates of biotype jawetz derived from mice, group b for the isolates of biotype jawetz derived from rats, and group c for the isolates of biotype heyl. these results suggest that the gyrb sequence of p. pneumotropica differs between the isolates of two biotypes ...	2006	17090967
outbreaks of avian cholera in hope bay, antarctica.	during austral summers 1999-2000 and 2000-01, two outbreaks of avian cholera occurred in the hope bay area (63 degrees 24's, 56 degrees 59'w), located on the tip of the antarctic peninsula. eighty-six dead birds were found: five kelp gulls (larus dominicanus), 36 skuas (stercorarius sp.), and 45 adelie penguins (pygoscelis adeliae). the carcasses were studied using clinical, pathological, and microbiological criteria. water samples from ponds where birds were settled and samples from 90 healthy ...	2006	16870848
seppic vaccine adjuvants for poultry.	two inactivated antigens (newcastle and pasteurella multocida) were formulated with different adjuvants and tested in two separate experiments in poultry. oil formulations constituting water in oil (w/o) or water in oil in water (w/o/w) emulsions were assessed for antibody response, protection, local reactions, and vaccine physicochemical parameters. robust, efficacious, and safe formulations were obtained with w/o formulations whereas w/o/w was especially safe with maintained efficacy. results ...	2006	17135512
separate pathways for o acetylation of polymeric and monomeric sialic acids and identification of sialyl o-acetyl esterase in escherichia coli k1.	o acetylation at carbon positions 7 or 9 of the sialic acid residues in the polysialic acid capsule of escherichia coli k1 is catalyzed by a phase-variable contingency locus, neuo, carried by the k1-specific prophage, cus-3. here we describe a novel method for analyzing polymeric sialic acid o acetylation that involves the release of surface sialic acids by endo-n-acetylneuraminidase digestion, followed by fluorescent labeling and detection of quinoxalinone derivatives by chromatography. the res ...	2006	16923886
gamma-glutamylcysteine synthetase-glutathione synthetase: domain structure and identification of residues important in substrate and glutathione binding.	in most organisms, glutathione (gsh) is synthesized by the sequential action of distinct enzymes, gamma-glutamylcysteine synthetase (gamma-gcs) and gsh synthetase (gs). in streptococcus agalactiae, gsh synthesis is catalyzed by a single enzyme, gamma-glutamylcysteine synthetase-glutathione synthetase (gamma-gcs-gs). the n-terminal sequence of gamma-gcs-gs is similar to escherichia coli gamma-gcs, but the c-terminal sequence is an atp-grasp domain more similar to d-ala, d-ala ligase than to any k ...	2006	16939198
multi-species patterns of avian cholera mortality in nebraska's rainwater basin.	nebraska's rainwater basin (rwb) is a key spring migration area for millions of waterfowl and other avian species. avian cholera has been endemic in the rwb since the 1970s and in some years tens of thousands of waterfowl have died from the disease. we evaluated patterns of avian cholera mortality in waterfowl species using the rwb during the last quarter of the 20th century. mortality patterns changed between the years before (1976-1988) and coincident with (1989-1999) the dramatic increases in ...	2006	16699151
[pasteurella dagmatis spondylodiscitis in a diabetic patient].	healthy pets contact are able to induce unusual and severe diseases.	2006	16978746
phylogenetic relationship of pasteurella pneumotropica isolates from laboratory rodents based on 16s rdna sequence.	a 1344 bp fragment of the 16s ribosomal dna (rdna) sequence was used to determine the genetic relationship of pasteurella pneumotropica isolates from laboratory rodents. a total of 30 nucleotide sequences of p. pneumotropica, including 24 wild strains, 3 reference strains, and 3 nucleotide sequences deposited in genbank, were examined for heterogeneity of their 16s rdna sequences. phylogenetic analysis based on 16s rdna sequence discriminated 5 types of branching lineages. of these 5 types, 3 ty ...	2006	16820726
fowl cholera outbreak in domestic poultry and epidemiological properties of pasteurella multocida isolate.	symptoms of fowl cholera including orofacial edema, swollen and edematous wattles and combs, and severe respiratory disorders were detected in domestic poultry in two broiler breeder farms: one located in gyeong-gi province (october, 2000) and the other in chung-cheong-nam province (march, 2001). gram-negative, bipolar staining bacillus was easily found in a direct smear. the biochemical properties of isolates were examined using a standard diagnosis method, proving that they were 99.7% similar ...	2006	16820765
characterization of a bacteriocin-like substance produced by bacillus amyloliquefaciens isolated from the brazilian atlantic forest.	a bacillus strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16s rdna sequencing. phylogenetic analysis indicated that the strain has high sequence similarity with bacillus amyloliquefaciens. the antimicrobial substance was inhibitory to pathogenic and food-spoilage bacteria, such as listeria monocytogenes, bacillus cereus, serratia marcescens, and pasteurella haemolytica. it was stable over a wide temperature range, but lost activity when the temperatu ...	2006	16835841
etiological agents of dairy cows' mastitis in western part of poland.	the purpose of the study was to determine the etiological mastitis agents in cows from herds located in the western part of poland in years 2003-2005. bacteriological examinations of 18,713 samples taken from california mastitis test (cmt) positive quarters were performed according to standard methods. some gram-negative bacilli and gram-positive cocci were examined with the use of api tests (analytical profile index). it was found that 32.7% of samples that were cmt positive were culture negati ...	2006	17020014
evolution of competence and dna uptake specificity in the pasteurellaceae.	many bacteria can take up dna, but the evolutionary history and function of natural competence and transformation remain obscure. the sporadic distribution of competence suggests it is frequently lost and/or gained, but this has not been examined in an explicitly phylogenetic context. additional insight may come from the sequence specificity of uptake by species such as haemophilus influenzae, where a 9 bp uptake signal sequence (uss) repeat is both highly overrepresented in the genome and neede ...	2006	17038178
distinct signaling functions for shc isoforms in the heart.	thrombin activates protease-activated receptor-1 (par-1) and engages signaling pathways that influence the growth and survival of cardiomyocytes as well as extracellular matrix remodeling by cardiac fibroblasts. this study examines the role of shc proteins in par-1-dependent signaling pathways that influence ventricular remodeling. we show that thrombin increases p46shc/p52shc phosphorylation at tyr(239)/tyr(240) and tyr(317) (and p66shc-ser(36) phosphorylation) via a pertussis toxin-insensitive ...	2006	16699171
[an unusual case of pulmonary granulomatous lesion in an immunocompetent patient: infection caused by pasteurella multocida].	we report a case of lung infection caused by pasteurella multocida in an immunocompetent patient. diagnosis was made both on histological analysis of a lung wedge resection, showing pyoepithelioid granulomas, and on bacteriological cultures from lung tissue. histological diagnosis of pulmonary pasteurellosis is difficult, and other etiological causes of pyoepithelioid granulomas of the lung have to be discussed. pulmonary pasteurellosis is particularly severe in immunocompromised patients, and s ...	2006	16841009
intrapleural heparin or heparin combined with human recombinant dnase is not effective in the treatment of empyema in a rabbit model.	the aim of this study was to investigate the effectiveness of intrapleural heparin or heparin combined with human recombinant dnase in the treatment of empyema.	2006	17052304
an unusual cause of sepsis and meningitis in a neonate.		2006	17055368
pasteurella multocida septicemia in a newborn without scratches, licks or bites.		2006	17058424
cross-talk between g(s)- and g(q)-coupled pathways in regulation of interleukin-4 by a(2b) adenosine receptors in human mast cells.	human mast cells express functional a(2a) and a(2b) adenosine receptors. however, only stimulation of a(2b), not a(2a), leads to secretion of interleukin (il)-4, an important step in adenosine receptor-mediated induction of ige synthesis by b-cells. in this study, we investigate intracellular pathways that link stimulation of a(2b) receptors to il-4 up-regulation in hmc-1 mast cells. both a(2a) and a(2b) receptors couple to g(s) proteins and stimulate adenylate cyclase, but only a(2b) stimulates ...	2006	16707627
acute suppurative thyroiditis caused by pasteurella multocida and associated with thyrotoxicosis.	acute suppurative thyroiditis (ast) is an uncommon condition of the thyroid gland. organisms of the staphylococcal and streptococcal species are the most commonly reported causative agents. rarely, ast has been associated with transient hyperthyroidism. we report a unique case of ast that was caused by pasteurella multocida and was associated with thyrotoxicosis in a previously healthy 51-year-old woman.	2006	16571095
pasteurella multocida pneumonia in an elderly non-immunocompromised man with a leg ulcer and exposure to cats.		2006	16864021
[molecularbiological diagnosis of herpes virus infection of a juvenile russian tortoise (agrionemys horsfieldii) with skin and lung lesions].	herpesvirus infections are significant in the care of turtles and tortoises. clinical signs range from unspecific symptoms, due to the variety of organ manifestations, to the "classical" picture of rhinitis-stomatitis. the presented case study showed the typical disease only with respect to clinical symptoms following hibernation, but lacks stomatitis, erosions or plaques in the oral mucosa. on the other hand, skin lesions on the extremeties, causative with herpesvirus infection, could be diagno ...	2006	16450705
concentration of airborne endotoxins and airborne bacteria in chinese rabbit houses.	concentration of airborne endotoxins, airborne aerobic bacteria and airborne aerobic gram-negative bacteria were measured in 3 rabbit houses. further, the species composition of the airborne gram-negative bacterial flora was investigated. the total amount of airborne endotoxin ranged from 22 to 774 eu/m3 (endotoxin units/m3). the number of total airborne aerobic bacteria varied between 780 and 20100 cfu/m3, the number of airborne aerobic gram-negative bacteria between 39 and 1030 cfu/m3. most gr ...	2006	16450707
distribution of rtx toxin genes in strains of [actinobacillus] rossii and [pasteurella] mairii.	strains of [actinobacillus] rossii, [pasteurella] mairii and [pasteurella] aerogenes can be isolated from abortion in swine. the rtx toxin pax has previously been found only in those [p.] aerogenes strains isolated from abortion. nothing is known about rtx toxins in field isolates of the other two species. to gain insight into the distribution of selected rtx toxin genes and their association with abortion, pcr screening for the pax, apxii and apxiii operons on 21 [a.] rossii and seven [p.] mair ...	2006	16621343
crystallization and preliminary x-ray crystallographic analysis of the alpha-2,6-sialyltransferase pm0188 from pasteurella multosida.	sialyltransferase is an enzyme that transfers the sialic acid moiety from cytidine-5-monophospho-n-acetylneuraminic acid (cmp-neuac) to the carbohydrate group of various glycoproteins. these glycoproteins are involved in inflammation, embryogenesis, immune defence and metastasis of cancer cells by cell-cell interactions or cell-matrix interactions. the alpha-2,6-sialyltransferase pm0188 from pasteurella multocida was purified using affinity-column chromatographic methods and crystallized using t ...	2006	16511286
structural characterization of salmonella typhimurium yeaz, an m22 o-sialoglycoprotein endopeptidase homolog.	the salmonella typhimurium "yeaz" gene (styeaz) encodes an essential protein of unknown function (styeaz), which has previously been annotated as a putative homolog of the pasteurella haemolytica m22 o-sialoglycoprotein endopeptidase gcp. yeaz has also recently been reported as the first example of an rpf from a gram-negative bacterial species. to further characterize the properties of styeaz and the widely occurring mk-m22 family, we describe the purification, biochemical analysis, crystallizat ...	2006	16617437
antimicrobial susceptibilities of pasteurella strains isolated from humans.	the in vitro susceptibilities of 192 consecutive clinical strains of pasteurella spp. isolated between 1996 and 2003 from soft tissue pus (n = 146), respiratory tract specimens (n = 38) and blood (n = 8) were studied by an agar dilution method. all isolates were susceptible to minocycline, cefotaxime, ofloxacin, ciprofloxacin and levofloxacin. most strains were susceptible to moxifloxacin, amoxicillin, azithromycin and clarithromycin, whereas lower susceptibility rates to telithromycin (89.4%) w ...	2006	16564680
biodegradation of dimethyl terephthalate by pasteurella multocida sa follows an alternative biochemical pathway.	pasteurella multocida sa, a bacterial strain isolated from mangrove sediment by enrichment technique, was capable of transforming dimethyl terephthalate (dmt). biodegradation of dmt was shown to take place as a series of sequential steps involving the hydrolysis of two ester linkages between the carboxyl groups of the terephthalate and the methyl side-chain initially to produce mono-methyl terephthalate (mmt) and then terephthalic acid (ta), respectively. however, with ethanol as the carrying so ...	2006	16676217
contribution of respiratory burst activity to innate immune function and the effects of disease status and agent on chemiluminescence responses by ruminant phagocytes in vitro.	the mechanisms of interaction between phagocytes and different bacteria that help resolve lung infections or contribute to lung pathology are poorly defined. alveolar phagocytes (resident macrophages and recruited neutrophils) make a major contribution to innate immunity by mounting a respiratory burst that helps kill internalised bacteria. however, this ability may be altered during or after exposure to infection. this review considers the application and limitations of a variety of analytical ...	2006	16678912
diseases and pathogens associated with mortality in ontario beef feedlots.	this study determined the prevalence of diseases and pathogens associated with mortality or severe morbidity in 72 ontario beef feedlots in calves that died or were euthanized within 60 days after arrival. routine pathologic and microbiologic investigations, as well as immunohistochemical staining for detection of bovine viral diarrhea virus (bvdv) antigen, were performed on 99 calves that died or were euthanized within 60 days after arrival. major disease conditions identified included fibrinos ...	2006	16566254
selection of immunodominant mimics of iromp-99 of rabbit pasteurella multocida from a random 12-peptide library.	a random 12-peptide library was used to screen immunodominant mimics of 99kda iron-regulated outer membrane protein (iromp-99) of rabbit pasteurella multocida. in the present study, expression of iromps of rabbit p. multocida strain c51-12 were analyzed by sds-page, and western blot to determine the specificity of rat antiserum antibodies against iromp-99. only iromp-99 whose expression was induced under iron-restricted conditions was detected on nitrocellulose paper. the phage display library w ...	2006	16584854
cat bite wound infections: a prospective clinical and microbiological study at three emergency wards in stockholm, sweden.	the rate of infection following cat bites appears to be greater than that from dog bites. to study the clinical picture, complications and microbiology (in humans and cats), this prospective study was performed.	2006	16483663
clonal stability of pasteurella multocida in free-range layers affected by fowl cholera.	detailed longitudinal studies of the genetic stability of pasteurella multocida ssp. multocida, the cause of fowl cholera, have not previously been carried out. consequently, the aim of the present study was to provide detailed information on the genetic stability and diversity of p. multocida ssp. multocida in poultry flocks over time, enabling new insights into the molecular epidemiology of this important poultry pathogen. longitudinal investigations of the rate and causes of mortality were ca ...	2006	16595311
molecular typing of pasteurella pneumotropica isolated from rodents by amplified 16s ribosomal dna restriction analysis and pulsed-field gel electrophoresis.	a total of 52 isolates of pasteurella pneumotropica obtained from rodents were examined for their genetic heterogeneity. on the basis of dna restriction analysis, including amplified 16s ribosomal dna restriction analysis (ardra) and pulsed-field gel electrophoresis (pfge), differences were identified among the isolates. ardra typing with hae iii revealed 4 different banding patterns of the p. pneumotropica isolates. eighty-two percent of the 23 isolates identified as a-1 were derived from mice, ...	2006	16625048
complete nucleotide sequence of a p2 family lysogenic bacteriophage, varphimhaa1-phl101, from mannheimia haemolytica serotype a1.	the 34,525 nucleotide sequence of a double-stranded dna bacteriophage (phimhaa1-phl101) from mannheimia haemolytica serotype a1 has been determined. the phage encodes 50 open reading frames. twenty-three of the proteins are similar to proteins of the p2 family of phages. other protein sequences are most similar to possible prophage sequences from the draft genome of histophilus somni 2336. fourteen open reading frames encode proteins with no known homolog. the p2 orthologues are collinear in phi ...	2006	16631219
evaluation of antigen panels for elisa monitoring of mouse colonies for antibodies to pasteurellaceae.	pasteurellaceae infection in mice may be monitored by the detection of serum antibody using enzyme-linked immunosorbent assay (elisa). we re-evaluated our standard antigen panel comprising pasteurella pneumotropica and a v-factor requiring haemophilus species (strain h21) by studying their serological relationship with actinobacillus muris and 'haemophilus influenzae-murium'. serologically, a. muris and 'h. influenzae-murium' were found to be unrelated and to differ from p. pneumotropica and hae ...	2006	16600079
evaluation of the prevalence and onset of lung lesions and their impact on growth of lambs.	to determine the prevalence and temporal onset of lung lesions in lambs and the impact of lung lesions on growth of affected lambs.	2006	16649926
plasmid-mediated florfenicol resistance in pasteurella trehalosi.	a florfenicol-resistant pasteurella trehalosi isolate from a calf was investigated for the presence and the location of the gene flor.	2006	16670108
effects of supplementation of beta-glucans on growth performance, nutrient digestibility, and immunity in weanling pigs.	two experiments were conducted to evaluate the efficacy of beta-glucan on growth performance, nutrient digestibility, and immunity in weanling pigs. in exp. 1, 210 weanling pigs (6.38 +/- 0.92 kg of bw) were fed dietary beta-glucan (0, 0.01, 0.02, 0.03, or 0.04%) for 5 wk. in exp. 2, 168 pigs (6.18 +/- 1.31 kg of bw) were fed no beta-glucan or antibiotics (t1), 0.02% beta-glucan (t2), only antibiotics (t3), or 0.02% beta-glucan with antibiotics (t4) for 8 wk. in exp. 2, the antibiotics fed were ...	2006	16699099