Publications

efficacy of neutral electrolyzed water for reducing pathogenic bacteria contaminating shrimp.	pathogenic contamination is a food safety concern. this study was conducted to investigate the efficacy of neutral electrolyzed water (new) in killing pathogens, namely, vibrio parahaemolyticus, vibrio vulnificus, salmonella enteritidis, and escherichia coli in shrimp. pure cultures of each pathogen were submerged separately in new containing five different chlorine concentrations: 10, 30, 50, 70, and 100 ppm. for each concentration, three submersion times were tested: 1, 3, and 5 min. the popul ...	2014	25474069
characterization of the vibrio vulnificus 1-cys peroxiredoxin prx3 and regulation of its expression by the fe-s cluster regulator iscr in response to oxidative stress and iron starvation.	peroxiredoxins (prxs) are ubiquitous antioxidant enzymes that reduce toxic peroxides. a new vibrio vulnificus prx, named prx3, was identified and characterized in this study. biochemical and mutational analyses revealed that prx3 reduces h2o2, utilizing glutaredoxin 3 (grx3) and glutathione (gsh) as reductants, and requires only n-terminal peroxidatic cysteine for its catalysis. these results, combined with the monomeric size of prx3 observed under non-reducing conditions, suggested that prx3 is ...	2014	25398878
distinct characteristics of oxyr2, a new oxyr-type regulator, ensuring expression of peroxiredoxin 2 detoxifying low levels of hydrogen peroxide in vibrio vulnificus.	two peroxiredoxins, prx1 and prx2, were previously identified in vibrio vulnificus. besides oxyr1, a homologue of escherichia coli oxyr (ecoxyr), oxyr2 that shares low homology with ecoxyr was first identified in v. vulnificus. oxyr2 activated prx2 during aerobic growth, while oxyr1 activated prx1 only when exposed to exogenous h2o2. oxyr2 was oxidized to form a reversible c206 to c215 disulphide bond by sensing low levels of h2o2, which were insufficient to oxidize oxyr1, and only the oxidized ...	2014	25041181
vibrio vulnificus mo6-24/o lipopolysaccharide stimulates superoxide anion, thromboxane b₂, matrix metalloproteinase-9, cytokine and chemokine release by rat brain microglia in vitro.	although human exposure to gram-negative vibrio vulnificus (v. vulnificus) lipopolysaccharide (lps) has been reported to result in septic shock, its impact on the central nervous system's innate immunity remains undetermined. the purpose of this study was to determine whether v. vulnificus mo6-24/o lps might activate rat microglia in vitro and stimulate the release of superoxide anion (o₂⁻), a reactive oxygen species known to cause oxidative stress and neuronal injury in vivo. brain microglia we ...	2014	24675728
functional analysis of tolc homologs in vibrio vulnificus.	gram-negative bacteria use tripartite pumps to transport antibacterial drugs and other toxic compounds across the inner and outer membranes, which are separated by the periplasmic space. the tolc protein is an outer membrane factor that participates in the formation of tripartite efflux pumps. the genome of vibrio vulnificus encodes two e. coli tolc homologs, tolcv1 and tolcv2. here, we show that both tolcv1 and tolcv2 are involved in the efflux of antimicrobial agents. deletion of tolcv1 result ...	2014	24515351
interaction between the α-barrel tip of vibrio vulnificus tolc homologs and acra implies the adapter bridging model.	the acrab-tolc multidrug efflux pump confers resistance to escherichia coli against many antibiotics and toxic compounds. the tolc protein is an outer membrane factor that participates in the formation of type i secretion systems. the genome of vibrio vulnificus encodes two proteins homologous to the e. coli tolc, designated tolcv1 and tolcv2. here, we show that both tolcv1 and tolcv2 partially complement the e. coli tolc function and physically interact with the membrane fusion protein acra, a ...	2014	24500479
pvv3, a new shuttle vector for gene expression in vibrio vulnificus.	an efficient electroporation procedure for vibrio vulnificus was designed using the new cloning vector pvv3 (3,107 bp). transformation efficiencies up to 2 × 10(6) transformants per μg dna were achieved. the vector stably replicated in both v. vulnificus and escherichia coli and was also successfully introduced into vibrio parahaemolyticus and vibrio cholerae. to demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (gfp) gene and the vvhba hemolysin oper ...	2014	24362421
peroxidase and photoprotective activities of magnesium protoporphyrin ix.	magnesium-protoporphyrin ix (mg-ppn), which is formed through chelation of protoporphyrin ix (ppn) with mg ion by mg chelatase, is the first intermediate for the (bacterio)chlorophyll biosynthetic pathway. interestingly, mg-ppn provides peroxidase activity (approximately 4 × 10(-2) units/micrometer) detoxifying h2o2 in the presence of electron donor(s). the peroxidase activity was not detected unless ppn was chelated with mg ion. mg-ppn was found freely diffusible through the membrane of escheri ...	2014	24317481
functional conservation of rnase iii-like enzymes: studies on a vibrio vulnificus ortholog of escherichia coli rnase iii.	bacterial ribonuclease iii (rnase iii) belongs to the rnase iii enzyme family, which plays a pivotal role in controlling mrna stability and rna processing in both prokaryotes and eukaryotes. in the vibrio vulnificus genome, one open reading frame encodes a protein homologous to e. coli rnase iii, designated vv-rnase iii, which has 77.9 % amino acid identity to e. coli rnase iii. here, we report that vv-rnase iii has the same cleavage specificity as e. coli rnase iii in vivo and in vitro. express ...	2014	24241331
antibacterial, antiviral, and antifungal properties of wines and winery byproducts in relation to their flavonoid content.	grapes produce organic compounds that may be involved in the defense of the plants against invading phytopathogens. these metabolites include numerous phenolic compounds that are also active against human pathogens. grapes are used to produce a variety of wines, grape juices, and raisins. grape pomace, seeds, and skins, the remains of the grapes that are a byproduct of winemaking, also contain numerous bioactive compounds that differ from those found in grapes and wines. this overview surveys an ...	2014	24945318
species differences in alternative substrate utilization by the antibacterial target undecaprenyl pyrophosphate synthase.	undecaprenyl pyrophosphate synthase (upps) is a critical enzyme required for the biosynthesis of polysaccharides essential for bacterial survival. in this report, we have tested the substrate selectivity of upps derived from the mammalian symbiont bacteroides fragilis, the human pathogen vibrio vulnificus, and the typically benign but opportunistic pathogen escherichia coli. an anthranilamide-containing substrate, 2-amideanilinogeranyl diphosphate (2aa-gpp), was an effective substrate for only t ...	2014	25020247
bacterial virulence analysis using brine shrimp as an infection model in relation to the importance of quorum sensing and proteases.	brine shrimp are aquatic crustaceans belonging to a genus of artemia. this organism is widely used for testing the toxicity of chemicals. in this study, brine shrimp were evaluated as an infection model organism to study bacterial virulence. artemia nauplii were infected with various pathogenic bacteria, such as vibrio vulnificus, pseudomonas aeruginosa, burkholderia vietnamiensis, staphylococcus aureus, and escherichia coli, and the susceptibility to these bacteria was investigated by counting ...	2014	25420421
dietary supplementation of bacillus subtilis and fructooligosaccharide enhance the growth, non-specific immunity of juvenile ovate pompano, trachinotus ovatus and its disease resistance against vibrio vulnificus.	a feeding trial was conducted to investigate the effects of dietary administration of probiotic bacillus subtilis and prebiotic fructooligosaccharide (fos) on growth performance, immune responses and disease resistance of juvenile ovate pompano, trachinotus ovatus. one thousand six hundred and twenty individuals (initial body weight: 10.32 ± 0.46 g, mean ± s.e) were fed nine practical diets according to a 3 × 3 factorial design: the basal diet as the control diet supplemented with three levels o ...	2014	24614017
iscr is a global regulator essential for pathogenesis of vibrio vulnificus and induced by host cells.	a mutant that exhibited less cytotoxic activity toward int-407 human intestinal epithelial cells than the wild type was screened from a random transposon mutant library of vibrio vulnificus, and an open reading frame encoding an fe-s cluster regulator, iscr, was identified using a transposon-tagging method. a mutational analysis demonstrated that iscr contributes to mouse mortality as well as cytotoxicity toward the int-407 cells, indicating that iscr is essential for the pathogenesis of v. vuln ...	2014	24478072
molecular characterization of vulnibactin biosynthesis in vibrio vulnificus indicates the existence of an alternative siderophore.	vibrio vulnificus is a halophilic estuarine bacterium that causes fatal septicemia and necrotizing wound infections in humans. virulent v. vulnificus isolates produce a catechol siderophore called vulnibactin, made up of one residue of 2, 3-dihydroxybenzoic acid (2, 3-dhba) and two residues of salicylic acid (sa). vulnibactin biosynthetic genes (vv2_0828 to vv2_0844) are clustered at one locus of chromosome 2, expression of which is significantly up-regulated in vivo. in the present study, we de ...	2014	24478763
host-nonspecific iron acquisition systems and virulence in the zoonotic serovar of vibrio vulnificus.	the zoonotic serovar of vibrio vulnificus (known as biotype 2 serovar e) is the etiological agent of human and fish vibriosis. the aim of the present work was to discover the role of the vulnibactin- and hemin-dependent iron acquisition systems in the pathogenicity of this zoonotic serovar under the hypothesis that both are host-nonspecific virulence factors. to this end, we selected three genes for three outer membrane receptors (vuua, a receptor for ferric vulnibactin, and hupa and hutr, two h ...	2014	24478087
monoclonal antibodies against vibrio vulnificus rtxa1 elicit protective immunity through distinct mechanisms.	vibrio vulnificus causes rapidly progressing septicemia with an extremely high mortality rate (≥50%), even with aggressive antibiotic treatment. the bacteria secrete multifunctional autoprocessing repeats-in-toxin (martx) toxins, which are involved in the pathogenesis of gram-negative vibrio species. recently, we reported that immunization with the c-terminal region of v. vulnificus rtxa1/martxvv, rtxa1-c, elicits a protective immune response against v. vulnificus through a poorly defined mechan ...	2014	25156730
vibrio vulnificus biotype 3 multifunctional autoprocessing rtx toxin is an adenylate cyclase toxin essential for virulence in mice.	vibrio vulnificus is an environmental organism that causes both food-borne and wound infections with high morbidity and mortality in humans. the annual incidence and global distribution of infections associated with this pathogen are increasing with climate change. in the late 1990s, an outbreak of tilapia-associated wound infections in israel was linked to a previously unrecognized variant of v. vulnificus designated biotype 3. the sudden emergence and clonality of the outbreak suggest that thi ...	2014	24614656
cytotoxicity of the vibrio vulnificus martx toxin effector duf5 is linked to the c2a subdomain.	the multifunctional-autoprocessing repeats-in-toxin (martx) toxins are bacterial protein toxins that serve as delivery platforms for cytotoxic effector domains. the domain of unknown function in position 5 (duf5) effector domain is present in at least six different species' martx toxins and as a hypothetical protein in photorhabdus spp. its presence increases the potency of the vibrio vulnificus martx toxin in mouse virulence studies, indicating duf5 directly contributes to pathogenesis. in this ...	2014	24935440
thermodynamic properties of the effector domains of martx toxins suggest their unfolding for translocation across the host membrane.	martx (multifunctional autoprocessing repeats-in-toxin) family toxins are produced by vibrio cholerae, vibrio vulnificus, aeromonas hydrophila and other gram-negative bacteria. effector domains of martx toxins cross the cytoplasmic membrane of a host cell through a putative pore formed by the toxin's glycine-rich repeats. the structure of the pore is unknown and the translocation mechanism of the effector domains is poorly understood. we examined the thermodynamic stability of the effector domai ...	2014	24724536
liposome-encapsulated cinnamaldehyde enhances zebrafish (danio rerio) immunity and survival when challenged with vibrio vulnificus and streptococcus agalactiae.	cinnamaldehyde, which is extracted from cinnamon, is a natural compound with activity against bacteria and a modulatory immune function. however, the antibacterial activity and immunostimulation of cinnamaldehyde in fish has not been well investigated due to the compound's poor water solubility. thus, liposome-encapsulated cinnamaldehyde (lec) was used to evaluate the effects of cinnamaldehyde on in vitro antibacterial activity against aquatic pathogens and in vivo immunity and protection parame ...	2014	24632045
skin and soft tissue infections following marine injuries and exposures in travelers.	bacterial skin and soft tissue infections (sstis) in travelers often follow insect bites and can present a broad spectrum of clinical manifestations ranging from impetigo to necrotizing cellulitis. significant sstis can also follow marine injuries and exposures in travelers, and the etiologies are often marine bacteria.	2014	24628985
cloning, crystallization and preliminary x-ray diffraction analysis of an intact dna methyltransferase of a type i restriction-modification enzyme from vibrio vulnificus.	independently of the restriction (hsdr) subunit, the specificity (hsds) and methylation (hsdm) subunits interact with each other, and function as a methyltransferase in type i restriction-modification systems. a single gene that combines the hsds and hsdm subunits in vibrio vulnificus yj016 was expressed and purified. a crystal suitable for x-ray diffraction was obtained from 25%(w/v) polyethylene glycol monomethylether 5000, 0.1 m hepes ph 8.0, 0.2 m ammonium sulfate at 291 k by hanging-drop va ...	2014	24699746
crystal structure of the full-length atpase gspe from the vibrio vulnificus type ii secretion system in complex with the cytoplasmic domain of gspl.	the type ii secretion system (t2ss) is present in many gram-negative bacteria and is responsible for secreting a large number of folded proteins, including major virulence factors, across the outer membrane. the t2ss consists of 11-15 different proteins most of which are present in multiple copies in the assembled secretion machinery. the atpase gspe, essential for the functioning of the t2ss, contains three domains (n1e, n2e and cte) of which the n1e domain is associated with the cytoplasmic do ...	2014	25092625
activities of alkyl hydroperoxide reductase subunits c1 and c2 of vibrio parahaemolyticus against different peroxides.	alkyl hydroperoxide reductase subunit c gene (ahpc) functions were characterized in vibrio parahaemolyticus, a commonly occurring marine food-borne enteropathogenic bacterium. two ahpc genes, ahpc1 (vpa1683) and ahpc2 (vp0580), encoded putative two-cysteine peroxiredoxins, which are highly similar to the homologous proteins of vibrio vulnificus. the responses of deletion mutants of ahpc genes to various peroxides were compared with and without gene complementation and at different incubation tem ...	2014	25239899
contribution of six flagellin genes to the flagellum biogenesis of vibrio vulnificus and in vivo invasion.	vibrio vulnificus is a halophilic pathogenic bacterium that is motile due to the presence of a single polar flagellum. v. vulnificus possesses a total of six flagellin genes organized into two loci (flafba and flacde). we proved that all six of the flagellin genes were transcribed, whereas only five (flaa, -b, -c, -d, and -f) of the six flagellin proteins were detected. to understand roles of the six v. vulnificus flagellins in motility and virulence, mutants with single and multiple flagellin d ...	2014	24101693
starvation induces phenotypic diversification and convergent evolution in vibrio vulnificus.	starvation is a common stress experienced by bacteria living in natural environments and the ability to adapt to and survive intense stress is of paramount importance for any bacterial population. a series of starvation experiments were conducted using v. vulnificus 93u204 in phosphate-buffered saline and seawater. the starved population entered the death phase during the first week and approximately 1% of cells survived. after that the population entered a long-term stationary phase, and could ...	2014	24551129
pre-existing medical conditions associated with vibrio vulnificus septicaemia.	vibrio vulnificus (vv) can result in severe disease. although pre-existing liver disease is a recognized risk factor for serious infection, the relative importance of other comorbidities has not been fully assessed. we analysed reports of vv infections submitted to cdc from january 1988 to september 2006 in order to assess the role of pre-existing conditions contributing to severe outcomes. a total of 1212 patients with vv infection were reported. only patients with liver disease [adjusted odds ...	2014	23842472
the blue fluorescent protein from vibrio vulnificus ckm-1 is a useful reporter for plant research.	the mbfp is an improved variant of nadph-dependent blue fluorescent protein that was originally identified from the non-bioluminescent pathogenic bacteria vibrio vulnificus ckm-1. to explore the application of mbfp in plants, the mbfp gene expression was driven by one of the three promoters, namely, leaf-specific (rbcs), hypoxia-inducible (adh) or auxin-inducible (dr5) promoters, in different plant tissues such as leaves, roots and flowers under diverse treatments. in addition, the expressed mbf ...	2014	28510958
implications of chitin attachment for the environmental persistence and clinical nature of the human pathogen vibrio vulnificus.	vibrio vulnificus naturally inhabits a variety of aquatic organisms, including oysters, and is the leading cause of seafood-related death in the united states. strains of this bacterium are genetically classified into environmental (e) and clinical (c) genotypes, which correlate with source of isolation. e-genotype strains integrate into marine aggregates more efficiently than do c-genotype strains, leading to a greater uptake of strains of this genotype by oysters feeding on these aggregates. t ...	2014	24362430
the evaluation of four recent culture-based methods for the isolation and enumeration of vibrio vulnificus bacteria from oyster meat.	the most common cause of seafood-borne death in the united states is the bacterium vibrio vulnificus which can be concentrated into high numbers in the tissues of oysters or other shellfish. the ability to quickly, accurately, and inexpensively isolate living strains of this organism from oyster tissues is crucial for effective research on this pathogen. in this report, we evaluate four methods for isolating and quantifying v. vulnificus from oyster tissues, the solid media cpc+ (a refined versi ...	2014	24355773
a bacterial flagellin in combination with proinflammatory cytokines activates human monocyte-derived dendritic cells to generate cytotoxic t lymphocytes having increased homing signals to cancer.	flagellin, the cognate ligand for toll-like receptor 5, has potent adjuvant activity in various vaccines. however, its efficacy in generating dendritic cells (dcs) remains contentious. this study assessed how efficaciously vibrio vulnificus flab (v-flab) could be used in generating a potent dc to induce antigen-specific cytotoxic t lymphocytes (ctls). mature dcs (mdcs) induced by the combination of v-flab/tnfα/ifnα were significantly more potent in inducing specific anticancer immune responses c ...	2014	24316552
a fish-stunning wound infection with acute cardiac injury.	vibrio vulnificus typically causes septicemia and necrotic wound infection. among v vulnificus–related complications, acute nonthrombotic myocardial damage has not been reported. the most effective antibiotic treatment of v vulnificus infection includes combination of a third-generation cephalosporin and a tetracycline or its analogue. however, recommendations of a fourth-generation cephalosporinbased regimen for treating the disease are not established. a 67-year-old diabetic man acquired v vul ...	2014	24176592
survival of vibrio vulnificus genotypes in male and female serum, and production of siderophores in human serum and seawater.	vibrio vulnificus is an estuarine bacterium responsible for 95% of all seafood-related deaths in the united states. several studies have demonstrated that v. vulnificus infections are enhanced when host iron availability is increased, such as occurs with chronic liver disease. because of the gender difference seen in these infections, we examined whether there was a difference in the survival in both male and female serum by both the c (clinical) and e (environmental) genotypes of v. vulnificus. ...	2014	24161025
differential expression of a sodium-phosphate cotransporter among vibrio vulnificus strains.	vibrio vulnificus is an estuarine bacterium with pathogenic potential. its three known biotypes differ in host distribution. we have found the npta gene for a sodium-phosphate cotransporter, which is rare in bacteria, in each biotype. npta transcript abundance differed significantly among biotypes, leading to the hypothesis that transcript levels differ under environmental conditions associated with estuarine and host environments. npta transcript abundance was assessed in v. vulnificus biotypes ...	2014	24141939
protection against vibrio vulnificus infection by active and passive immunization with the c-terminal region of the rtxa1/martxvv protein.	vibrio vulnificus is a foodborne pathogen that is prevalent in coastal waters worldwide. infection with v. vulnificus causes septicemia with fatality rates exceeding 50% even with aggressive antibiotic therapy. several vaccine studies to prevent v. vulnificus infection have been performed but have had limited success. in this study, we identified the c-terminal region (amino acids 3491 to 4701) of the v. vulnificus multifunctional autoprocessing rtx (martxvv or rtxa1) protein, rtxa1-c, as a prom ...	2014	24252692
zernike phase contrast cryo-electron tomography of whole bacterial cells.	cryo-electron tomography (cryo-et) provides three-dimensional (3d) structural information of bacteria preserved in a native, frozen-hydrated state. the typical low contrast of tilt-series images, a result of both the need for a low electron dose and the use of conventional defocus phase-contrast imaging, is a challenge for high-quality tomograms. we show that zernike phase-contrast imaging allows the electron dose to be reduced. this limits movement of gold fiducials during the tilt series, whic ...	2014	24075950
effects of temperature, growth phase and luxo-disruption on regulation systems of toxin production in vibrio vulnificus strain l-180, a human clinical isolate.	vibrio vulnificus is a halophilic estuarine bacterium while it causes fatal septicemia or necrotizing wound infections in humans. this pathogen secretes the metalloprotease (v. vulnificus protease: vvp) and the cytolysin (v. vulnificus hemolysin: vvh) as protein toxins; however, their production was coordinated in response to the bacterial cell density. this regulation is termed quorum sensing (qs) and is mediated by the small diffusible molecule called autoinducer 2 (ai-2). in the present study ...	2014	24068537
controlling vibrio vulnificus and spoilage bacteria in fresh shucked oysters using natural antimicrobials.	this study evaluated the efficacy of grape seed extract (ge), citric acid (ca) and lactic acid (la) on the inactivation of vibrio vulnificus and inherent microflora in fresh shucked oysters. the minimum inhibitory concentration (mic) of ge, ca or la against v. vulnificus was determined. furthermore, the shucked oysters were artificially inoculated with v. vulnificus. the inoculated shucked oysters (25 g) were then dipped in 250 ml ge, ca or la solutions for 10 min. the population of v. vulnificu ...	2014	24001001
[the intratypical differentiation of strains of the poliovirus group (marker determination)].		2014	4295632
plaque scores related to long-term results of periodontal therapy.		2014	4272235
letter: renal function and electrolyte disturbances in normocalcaemic and hypercalcaemic patients treated with mithramycin.		2014	4277561
letter: renal function and electrolyte disturbances in normocalcaemic and hypercalcaemic patients treated with mithramycin.		2014	4277561
[rotating total endoprosthesis of the hip joint].		2014	4248757
[fractures of amputation stumps and amputated limbs].		2014	4220975
[drinking fluoridated water for caries prophylaxis-- yes or no?].		2014	4245970
practical method of speech evaluation in adult patients with cleft lip and cleft palate.		2014	4187644
letter: plasma-catecholamines in sleeping hypertensives.		2014	4136084
[external respiratory function and indices of the acid-base state in combined liver and lung diseases].		2014	4094392
type ii diabetes mellitus.		2014	4024639
[removal of a phytobezoar during gastroscopy].		2014	4060482
blood transfusion and athletics. games people play.		2014	3974666
higher cardol homologues (5-alkenylresorcinols) from rye affect the red cell membrane-water transport.	the influence of 5-heptadecenylresorcinol and total rye 5-alkenylresorcinols isolated from rye grains on the red blood cell water permeability was studied using osmotic shrinkage experiments performed in 300 mm sucrose. the studied compounds induced significant increase of erythrocyte water permeability. the threshold concentration needed for the increase of water permeability was in an order of 10(-6) mol/l. the temperature dependence of the observed process showed the discontinuity which was r ...	2014	3993182
immature teratoma of the ovary with gliomatosis peritonei. a case report.	gliomatosis peritonei is a rare complication of immature teratoma of the ovary and should not be confused with metastatic ovarian carcinoma. treatment depends on the histological grading of the gliomatous lesions. all grades, except grade 0, qualify for adjuvant chemotherapy. repeated laparotomies for cytological sampling and the removal of tumour are essential. a 16-year-old ovambo nulligravida presenting with gliomatosis peritonei was apparently cured after 5 laparotomies for removal of tumour ...	2014	4012467
[the density distribution of the chromatin within the cell nucleus (author's transl)].		2014	4130775
iohexol vs. metrizamide: study of efficacy and morbidity in cervical myelography.	a double-blind study was conducted in 60 patients undergoing either cervical or more complete myelography via c1-c2 puncture. patients received either iohexol or metrizamide at a 300 mg l/ml concentration. the contrast media were equally efficacious in the production of high-quality radiographs and ct scans. however, the incidence of adverse reactions differed markedly. of patients receiving metrizamide, 68% had some type of adverse reaction, whereas only 26% receiving iohexol had symptoms. the ...	2014	3934932
[treatment of carotido-cavernous fistulae].		2014	4433151
pathophysiology of low density lipoprotein and high density lipoprotein glucosylation.		2014	3911060
[application of microassay to laboratory medicine--immunoassay in laboratory tests].		2014	3903286
[effects of intravenous perfusion of adrenalin on hyperlactacidemia from exertion in humans].		2014	4131723
values and structure in the german health care systems.		2014	3911098
[pacemaker implantation in infants and children for atrioventricular block].		2014	3819491
murine typhus, a report of 15 cases.		2014	4735083
[case of hurler's disease].		2014	4267654
psychological studies of longer range l-dopa therapy in parkinsonism.		2014	4148472
immunodeficiency syndromes and the gut.	the gastrointestinal tract in immunodeficiency disorders is involved with diseases similar to those seen in the immune competent. however, in some immune deficient states the incidence of gastrointestinal disease is high whereas in others it is no different than in the general population. this review clarifies the unique and specific abnormalities seen in immune deficient states and stresses the newest recognised abnormalities. it highlights the fact that the most frequent and severe abnormaliti ...	2014	3911369
spontaneous reactions of 1,3-substituted 1,4-dihydropyridines with acids in water at neutrality. ii. nuclear magnetic resonance studies.		2014	4249331
study of the uv-induction of mutations in bacteria.		2014	4221844
physiology of the upper airways in allergic disease.		2014	3902190
an unusual cause of neck swelling in an elderly female.		2014	4083241
[the 58th annual meeting of the japanese orthopedic association. gifu, april 7-9, 1985. abstracts].		2014	4078426
[mechanisms of adaptation to physical exertion in patients with an expiratory obstructive syndrome].		2014	4096445
[sequential angio-urography (sau) with photographic image subtraction].	urography was performed in 250 patients: the technique of bolus injection of a large quantity of contrast medium was used in every case. by means of rapid seriography the early vascular phase was shown and reproduced, when necessary, with image subtraction. the abdominal aorta and the renal arteries were demonstrated in 97.6% of cases. altogether, in 34.4% of cases the existence of pathological conditions non demonstrable by conventional urography was ascertained; in 12.4% of cases extrarenal pa ...	2014	3911305
[ataxia-telangiectasia (louis-bar syndrome)].		2014	4177264
metabolism of 1-naphthol by tyrosinase.	1-naphthol was metabolized by the polyphenol oxidase, tyrosinase, primarily to 1,2-naphthoquinone and to small amounts of 1,4-naphthoquinone as well as to covalently bound products. the inhibition of covalent binding by ethylenediamine, which reacts specifically with 1,2-naphthoquinone but not 1,4-naphthoquinone, suggested that most of the covalent binding was due to 1,2-naphthoquinone or a metabolite of similar structure. the activation by tyrosinase of 1-naphthol to covalently bound products s ...	2014	3929786
letter: brain-levels of monoamine oxidase in depression.		2014	4136104
[determination of anionic surface-active substances in the blood serum by a colorimetric method].		2014	3957060
the use of multivariate techniques in the study of skeletal populations.		2014	4313256
[new studies on a relationship between hemoglobins and rhesus factors in africans].		2014	4308643
[new studies on a relationship between hemoglobins and rhesus factors in africans].		2014	4308643
axillo-internal carotid artery bypass in the treatment of neck metastases.	the initial surgical management of neck metastases from head and neck cancer may be complicated if the tumor mass invades the carotid vessels. acute carotid ligation is associated with a high stroke rate. consequently preservation of flow to the internal carotid artery is desirable. we will describe two patients in whom preservation of flow was accomplished with the use of a saphenous vein bypass from the axillary artery to the internal carotid artery. the technique is described and has several ...	2014	4009839
free radicals of biological interest. ii. electron spin resonance (esr) spectra of 2537 a irradiated amino acids.		2014	4295529
free radicals of biological interest. ii. electron spin resonance (esr) spectra of 2537 a irradiated amino acids.		2014	4295529
[ultrastructural characteristics of lamellar exfoliation of the newborn. report of 2 cases].		2014	4615293
[qualitative cytology of the cerebrospinal fluid in primary cerebral atrophy].		2014	4249221
psychological impact of acne in adolescence.		2014	4239357
full mouth restoration under general anesthesia in pedodontic practice.		2014	4229229
the value of unionization to faculty.		2014	4492430
the value of unionization to faculty.		2014	4492430
[fascial fat herniation causing backache].		2014	4223461
simplified methods for automated ion-exchange separation of peptides and accelerated manual edman degradations.		2014	4202981
[red noses (author's transl)].		2014	4282887
[use of the levin method for laboratory diagnosis of liver cirrhosis].		2014	4183885
the role of radiology in dentistry for children.		2014	4225584
changes of the sella turcica in chromophobic adenomas and eosinophilic adenomas.		2014	4286984
the prevention of dental caries by mouthrinsing with solutions of neutral sodium fluoride.		2014	4149260
control of diesel exhaust odors.		2014	4138799
[the cause of volkmann's contracture--a primary neurotrophic complication of injuries in children].		2014	4188995
development and application of a model for estimating alveolar and interstitial dust levels.		2014	4091370
observations in petit mal status.		2014	4166754