Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| distribution and expression analysis of transcription factors in tissues and progenitor cell populations of the goldfish (carassius auratus l.) in response to growth factors and pathogens. | we report on the mrna levels of a panel of transcription factors in the kidney and spleen tissues, and in the cell populations from the blood, the spleen, and in the sorted kidney progenitor cells. the mrna levels of cebpα, cjun, cmyb, egr1, gata1, gata2, gata3, lmo2, mafb, pax5, pu.1 and runx1 were assessed in healthy goldfish as well as in fish challenged with two different pathogens, aeromonas salmonicida a449 or trypanosoma carassii. spleen tissue from healthy goldfish showed higher expressi ... | 2011 | 21474183 |
| gene expression profiles of the spleen, liver, and head kidney in turbot (scophthalmus maximus) along the infection process with aeromonas salmonicida using an immune-enriched oligo-microarray. | we evaluated the expression profiles of turbot in the spleen, liver, and head kidney across five temporal points of the aeromonas salmonicida infection process using an 8 × 15 k agilent oligo-microarray. the microarray included 2,176 different fivefold replicated gene probes designed from a turbot 3' sequenced est database. we were able to identify 471 differentially expressed (de) genes (17.3% of the whole microarray), 223 in the spleen, 246 in the liver, and 125 in the head kidney, in at least ... | 2011 | 21503602 |
| identification and epidemiological relationships of aeromonas isolates from patients with diarrhea, drinking water and foods. | a collection of aeromonas isolates obtained over a three-year period in the same geographic area (león, nw of spain) was characterized by (gtg)(5)-pcr fingerprinting, amplified fragment length polymorphism (aflp) analysis and gyrb gene sequence analysis. the isolates originated from human diarrheal stools (29 isolates), potable water (13 isolates), rabbit meat (13 isolates) and marine fish (5 isolates). the distribution of aeromonas species varied with the strain source. aeromonas caviae hg4 and ... | 2011 | 21550680 |
| alteration of virulence factors and rearrangement of pasa5 plasmid caused by the growth of aeromonas salmonicida in stressful conditions. | aeromonas salmonicida, a fish pathogen, is the causative agent of furunculosis. it was already shown that growing this bacterium in stressful conditions such as temperature above 22°c might lead to virulence attenuation. unfortunately, many veterinary microbiology services and reference centers still routinely cultivate a. salmonicida at 25°c. here we tested the presence of virulence factors by growth on specific medium as well as the integrity of the pasa5 plasmid, which bears an important viru ... | 2011 | 21621930 |
| characterization of granulocyte colony stimulating factor receptor of the goldfish (carassius auratus l.). | granulocyte colony stimulating factor receptor (gcsfr) is a member of the class i cytokine receptor superfamily and signaling through this receptor is important for the proliferation, differentiation and activation of neutrophils and their precursors. in this study we report on the cloning and molecular characterization of goldfish gcsfr. the identified goldfish gcsfr sequence possesses the conserved ig-like domain, the cytokine receptor homology domain (crh), three fibronectin domains as well a ... | 2011 | 21801744 |
| molecular basis of sulfonamide and trimethoprim resistance in fish-pathogenic aeromonas isolates. | sulfonamide-trimethoprim-resistant aeromonas salmonicida and motile aeromonas spp. from diseased fish of the germ-vet study carried the sul1 gene together with mostly cassette-borne trimethoprim resistance genes, including the novel gene dfra28. the seven dfra and dfrb genes identified were mostly located in class 1 integrons which commonly harboured other gene cassettes. | 2011 | 21764945 |
| a unique epidermal mucus lectin identified from catfish (silurus asotus): first evidence of intelectin in fish skin slime. | the present study reports a new type of skin mucus lectin found in catfish silurus asotus. the lectin exhibited calcium-dependent mannose-binding activity. when mannose eluate from chromatography with mannose-conjugated agarose was analyzed by sds-page, the lectin appeared as a single 35-kda band. gel filtration showed that the lectin forms monomers and dimers. a 1216-bp cdna sequence obtained by race-pcr from the skin encoded a 308 amino acid secretory protein with homology to mammalian and fis ... | 2011 | 21757471 |
| protection against atypical aeromonas salmonicida infection in common carp, cyprinus carpio l., by oral administration of a mixed microbial culture of lactobacillus paracasei, pichia membranifaciens and saccharomyces cereviciae. | a microbial culture was prepared by co-cultivation of lactobacillus paracasei, pichia membranifaciens and saccharomyces cereviciae for 48 hr at 30 c in rice bran extract medium, supplemented with dextrose. oral administration of the resulting non-viable heat-inactivated microbial culture to common carp, cyprinus carpio l., delivered in feed for four weeks, induced effective protection against experimental atypical aeromonas salmonicida infection which causes "ulcer disease". after challenge of t ... | 2011 | 21685718 |
| Bacteriophages with potential for inactivation of fish pathogenic bacteria: survival, host specificity and effect on bacterial community structure. | Phage therapy may represent a viable alternative to antibiotics to inactivate fish pathogenic bacteria. Its use, however, requires the awareness of novel kinetics phenomena not applied to conventional drug treatments. The main objective of this work was to isolate bacteriophages with potential to inactivate fish pathogenic bacteria, without major effects on the structure of natural bacterial communities of aquaculture waters. The survival was determined in marine water, through quantification by ... | 2011 | 22163184 |
| Identification of differentially expressed protective genes in liver of two rainbow trout strains. | Since 1975, the rainbow trout strain BORN (Germany) has been bred in brackish water from a coastal form imported from Denmark. Accompanying phenotypic monitoring of the adapted BORN trout until now revealed that this selection strain manifested a generally elevated resistance towards high stress and pathogenic challenge including lower susceptibility towards Aeromonas salmonicida infections in comparison to other trout strains in local aqua farms. We focus on the elucidation of both, genetic bac ... | 2011 | 22196148 |
| complete genomic sequence of a t4-like bacteriophage, phias4, infecting aeromonas salmonicida subsp. salmonicida. | a newly identified virulent phage (named phias4) infecting aeromonas salmonicida subsp. salmonicida was isolated from river water in korea. morphological analysis of phias4 by transmission electron microscopy revealed that it belonged to the family myoviridae. the genome of phias4 comprised a linear double-stranded dna of 163,875 bp with a g + c content of 41.3%, and genomic analysis revealed 271 putative orfs, 67 putative promoters, 25 putative terminator regions, and 16 trna-encoding genes. mo ... | 2011 | 22116495 |
| impact of pharmacist intervention on oral theophylline therapy in adult inpatients. | the impact of clinical pharmacy services on the utilization of oral theophylline therapy was evaluated in a ten-week study involving 138 adult inpatients. the study initially involved an independent prospective five-week audit of theophylline use, in which clinical pharmacists monitored theophylline therapy and any interventions were designed so as not to influence future actions taken by medical officers with regard to oral theophylline therapy. the second part of the study involved active inte ... | 2011 | 3428141 |
| ventricular arrhythmias in normal individuals and athletes. | 2011 | 3042408 | |
| pain!! | 2011 | 3271864 | |
| the impaired nurse: recovery & re-entry to practice. | 2011 | 3194859 | |
| [enquête survey on sudden deafness and comparison between the criteria for prognosis and subjective improvement]. | 2011 | 3199252 | |
| the joint task group on clinical utility of labelled antibodies of the society of nuclear medicine europa (snme) and the european nuclear medicine society (enms). | in september 1985 a task group on the clinical utility of labelled antibodies was founded by the society of nuclear medicine - europe. this document was elaborated to report the aims and the programs of the group. in september 1986 the european nuclear medicine society decided to participate in this initiative and a joint task group was created. initial document: a. the aims to the task group are to monitor, co-ordinate and promote research and clinical application of techniques using radiolabel ... | 2011 | 3429948 |
| neuroendocrine hypothesis of antidepressant action of ect. | 2011 | 3153500 | |
| [experience with the co2 laser in oral surgery]. | 2011 | 3126482 | |
| melanoma antigen expression and metastatic ability of mutant b16 melanoma clones. | the biological functions of murine melanoma-associated antigens recognized by monoclonal antibodies (mabs) (m562, m622 and m2590) were examined by using mutant clones which differed in their degree of expression of these antigens. four clones of high expressors of 3 types of antigen (mea group), 5 clones of low or non-expressors of m562- and m622-recognizing antigens (meb group) and 4 clones of non-expressor of gm3 recognized by m2590 (mec group) were used. attachment of these clones to componen ... | 2011 | 3053466 |
| the occurrence of aeromonas in drinking water, tap water and the porsuk river. | the occurrence of aeromonas spp. in the porsuk river, public drinking water and tap water in the city of eskisehir (turkey) was monitored. fresh water samples were collected from several sampling sites during a period of one year. total 102 typical colonies of aeromonas spp. were submitted to biochemical tests for species differentiation and of 60 isolates were confirmed by biochemical tests. further identifications of isolates were carried out first with the vitek system (biome˜rieux) and then ... | 2011 | 24031613 |
| a microelectrode study of the mechanisms of l-lactate entry into and release from frog sartorius muscle. | 1. changes in intracellular ph and intracellular anion levels were monitored in frog sartorius muscle fibres during exposure to extracellular l-lactate, using ion-sensitive microelectrodes. 2. resting intracellular ph (phi) in 20 mmol l-1 hepes buffer was 7.18 +/- 0.015 (s.e. of mean, n = 62). exposure to an extracellular solution at ph 6.5 buffered with 20 mmol l-1 3-(n-morpholino)propanesulphonic acid (mops) resulted in a slow intracellular acidification. 3. a reversible decrease in phi and an ... | 2011 | 3262155 |
| [new accidental causes of head injuries in skiing--a case report]. | 2011 | 3238403 | |
| immunohistochemical localizations of ca 125, carcinoembryonic antigen, and ca 19-9 in normal and neoplastic glandular cells of the uterine cervix. | immunohistochemical reactivities with ca 125, carcinoembryonic antigen (cea), and ca 19-9 were studied in 15 cervixes without histologic abnormalities, ten with microglandular hyperplasia, nine adenocarcinomas in situ, ten adenocarcinomas with an invasion of less than 5 mm in depth, and 25 frankly invasive adenocarcinomas of the uterine cervix. the glandular cells of the normal cervix and microglandular hyperplasia always exhibited reactivity with anti-ca 125 in the luminal surfaces and the secr ... | 2011 | 3191457 |
| [therapeutic recommendations in reversible myocardial ischemia-- angina pectoris]. | 2011 | 3199929 | |
| [rheovasography of the burned hand]. | 2011 | 3404837 | |
| acute neurogenic pulmonary oedema following generalized tonic clonic seizure. a case report and a review of the literature. | a case of acute neurogenic pulmonary oedema following a generalized tonic clonic seizure is presented. the condition is rare and the exact pathophysiology is unknown. pulmonary oedema is a serious complication of epileptic seizures, however, and the importance of awareness of this condition and its treatment is emphasized by a high mortality rate in older epileptics and by the demonstration of pulmonary oedema in recent series of young epileptics who died suddenly. | 2011 | 3053177 |
| homology of drosophila yolk proteins and the triacylglycerol lipase family. | drosophila yolk proteins consist of a set of related proteins of 50,000 mr. they are derived from slightly larger precursors by cleavage of a signal peptide. in this respect, they differ from the yolk proteins of other insects which are proteolytic fragments of precursors of 200,000 mr or larger, termed vitellogenins and probably homologous to the vitellogenins of other egg-laying species. we report here a comparative amino acid analysis demonstrating that the drosophila yolk proteins are non-ho ... | 2010 | 3139889 |
| homology of drosophila yolk proteins and the triacylglycerol lipase family. | drosophila yolk proteins consist of a set of related proteins of 50,000 mr. they are derived from slightly larger precursors by cleavage of a signal peptide. in this respect, they differ from the yolk proteins of other insects which are proteolytic fragments of precursors of 200,000 mr or larger, termed vitellogenins and probably homologous to the vitellogenins of other egg-laying species. we report here a comparative amino acid analysis demonstrating that the drosophila yolk proteins are non-ho ... | 2010 | 3139889 |
| effect of epidermal growth factor on synthesis of prostaglandins and cyclic amp by embryonic palate mesenchymal cells. | the influence of epidermal growth factor (egf) on the ability of murine embryonic palate mesenchymal (mepm) cells to be stimulated to synthesize camp and prostaglandins was investigated. preincubation of mepm cells with egf enhanced, in a dose-dependent fashion, (1) the responsiveness of mepm cells to prostaglandin e1-induced elevation of intracellular levels of camp, and (2) the responsiveness of cells to calcium ionophore (a23187) and melittin-induced synthesis of prostaglandins e2 and f2 alph ... | 2010 | 2992494 |
| granular cell myoblastoma of the breast. | 2010 | 2991509 | |
| oesophageal motility and lower oesophageal sphincter competence in progressive systemic sclerosis and localized scleroderma. | oesophageal motility and lower oesophageal sphincter (los) competence were investigated in 13 patients with progressive systemic sclerosis (pss) and in 16 patients with localized scleroderma (ls) by means of oesophageal manometry and 24-h ph monitoring of the distal oesophagus. results were compared with those of a control group consisting of asymptomatic volunteers. marked abnormalities in oesophageal motility and in acid exposure in the distal oesophagus were observed in pss patients only. the ... | 2010 | 2928728 |
| rigid fixation and bone grafts in craniofacial surgery. | 2010 | 2924485 | |
| macrophages as effector cells. introduction. | 2010 | 2996946 | |
| blood lead levels among office workers--new york city. | 2010 | 3084941 | |
| blood lead levels among office workers--new york city. | 2010 | 3084941 | |
| individual responses to converting enzyme inhibitors and calcium antagonists. | this study was designed to assess whether the acute blood pressure response of an individual hypertensive patient to a calcium antagonist or an angiotensin converting enzyme (ace) inhibitor is a good predictor of the long-term efficacy of these drug classes in this particular patient. the concept that good responses to ace inhibitors and calcium antagonists may be mutually exclusive was also tested. sixteen patients were included in a randomized crossover trial of enalapril, 20 mg daily, and dil ... | 2010 | 2830189 |
| [non-invasive diagnosis of pre- and postpartum urination disorders]. | postpartal disorders of urinary discharge due to birth trauma are frequently the cause of rising infections of the urinary tract during the puerperium. in diagnosis, noninvasive methods should be preferred. bladder-emptying function was checked by means of uroflowmetry and sonographic measurement of residual urine in 83 patients at the department of gynecology and obstetrics at the rwth, aachen, in the third trimester and post partum. a considerable restriction of urine flow was found both prepa ... | 2010 | 3041696 |
| [non-invasive diagnosis of pre- and postpartum urination disorders]. | postpartal disorders of urinary discharge due to birth trauma are frequently the cause of rising infections of the urinary tract during the puerperium. in diagnosis, noninvasive methods should be preferred. bladder-emptying function was checked by means of uroflowmetry and sonographic measurement of residual urine in 83 patients at the department of gynecology and obstetrics at the rwth, aachen, in the third trimester and post partum. a considerable restriction of urine flow was found both prepa ... | 2010 | 3041696 |
| peculiarities of the endocrine time structure in noninsulin-dependent adult-onset (type ii) diabetes mellitus. | twenty noninsulin-dependent elderly diabetic patients, ten of whom were treated by oral hypoglycemic agents and ten of whom were regulated by diet alone, and 20 clinically healthy subjects matched for age, sex, height, and weight were examined with six blood and six urine samples at 4-hr intervals over a 24-hr span. plasma acth, cortisol, aldosterone, and dehydroepiandrosterone-sulfate (dhea-s) were determined by radioimmunoassay (ria); epinephrine, norepinephrine, and dopamine in urine were det ... | 2010 | 3037559 |
| cyclophosphamide, doxorubicin and cisplatin chemotherapy for patients with locally advanced urothelial tumors with or without nodal metastases. | thirty-eight patients with primary urothelial tumors recurring in the pelvis or nodal presentation were treated with intravenous or intra-arterial cyclophosphamide, doxorubicin and cisplatin chemotherapy. the 38 patients were selected owing to unresectability by local criteria (12 patients) or by virtue of nodal metastases (26 patients). histologically, the patients either had pure transitional cell carcinoma (29), transformation to a histological subtype of transitional cell carcinoma (7) or pu ... | 2010 | 2993671 |
| [how can bivalves continue to close the shells?--regulation and a molecular mechanism on the economical catch contraction]. | 2010 | 2976172 | |
| malignant melanoma. | 2010 | 3768643 | |
| characterization of gp21 and gp12: two potential probiotic bacteria isolated from the gastrointestinal tract of atlantic cod. | this study evaluated the probiotic potential of gp21 (pseudomonas sp.) and gp12 (psychrobacter sp.), two bacteria isolated from the intestinal tract of a cold-water fish, atlantic cod. the antagonistic activity of the two intestinal bacteria against two fish pathogens (vibrio anguillarum and aeromonas salmonicida subsp. salmonicida) was studied under different physical conditions. further, their resistance to physiological barriers and their ability to form biofilms were examined. in addition, a ... | 2010 | 26781122 |
| enterobacteriaceae associated with eggs of podocnemis expansa and podocnemis unifilis (testudines:chelonia) in nonpolluted sites of national park of araguaia plains, brazil. | fertile eggs of podocnemis expansa and podocnemis unifilis were investigated for the presence of enterobacteria, as these two endangered species have the potential for conservation measures that include egg transfer. knowledge of normal microflora associated with turtles and turtle eggs would help effectively manage the transfer of these eggs among institutions. thirty eggs of each species were collected, aseptically transferred, cracked inside plastic bags containing tetrathionate broth, and sp ... | 2010 | 21370647 |
| influence of alginic acid and fucoidan on the immune responses of head kidney leukocytes in cod. | the present study investigated the immunomodulatory activities of alginic acid and fucoidan, both derived from brown seaweeds, on selected cellular immune responses and antibacterial activity of head kidney (hk) leukocytes of cod, gadus morhua. primary cultures of hk leukocytes were incubated with either 10 or 100 μg ml(-1) of the substances and the effects on respiratory burst, cellular proliferation, acid and alkaline phosphatase activity and cellular myeloperoxidase were measured at 3- and 24 ... | 2010 | 21191809 |
| mortality of centrarchid fishes in the potomac drainage: survey results and overview of potential contributing factors. | skin lesions and spring mortality events of smallmouth bass micropterus dolomieu and selected other species were first noted in the south branch of the potomac river in 2002. since that year morbidity and mortality have also been observed in the shenandoah and monocacy rivers. despite much research, no single pathogen, parasite, or chemical cause for the lesions and mortality has been identified. numerous parasites, most commonly trematode metacercariae and myxozoans; the bacterial pathogens aer ... | 2010 | 21192549 |
| the effects of the alkyl polycyclic aromatic hydrocarbon retene on rainbow trout (oncorhynchus mykiss) immune response. | the objective of this study was to examine the immune toxicity of the alkyl polycyclic aromatic hydrocarbon (pah) retene (7-isopropyl-1-methyl phenanthrene) in rainbow trout. retene is a common alkyl pah associated with combustion of terrestrial plants or industrial effluents. rainbow trout were injected intraperitoneally with a single dose of 0, 1, or 10mg/kg of retene and sampled at 21d. within each retene treatment, co-injection of formalin-killed aeromonas salmonicida or a phosphate buffered ... | 2010 | 20810174 |
| in vitro adherence of two candidate probiotics from atlantic cod and their interference with the adhesion of two pathogenic bacteria. | the potential of two candidate probiotic bacteria (gp21 and gp12), isolated from the gut of atlantic cod, to adhere to primary cultures of the epithelial cells from the different regions of the intestine and to interfere with the adhesion of two pathogens, vibrio anguillarum and aeromonas salmonicida subsp. salmonicida were investigated. the intestinal isolates showed clear preference in adhering to the cells from the different intestine segments. gp12 adhered strongly to the fore- and mid intes ... | 2010 | 20884135 |
| potential of the melanophore pigment response for detection of bacterial toxicity. | chromatophore cells have been investigated as potential biodetectors for function-based detection of chemically and biologically toxic substances. oncorhynchus tshawytscha (chinook salmon) melanophores, a chromatophore cell type containing brown pigment, rapidly detect the salmonid pathogens aeromonas salmonicida, yersinia ruckeri, and flavobacterium psychrophilum and the human pathogen bacillus cereus. | 2010 | 20952639 |
| characterization and expression studies of gaduscidin-1 and gaduscidin-2; paralogous antimicrobial peptide-like transcripts from atlantic cod (gadus morhua). | piscidins are a family of antimicrobial peptides (amps) from fish that constitute an important component of their innate immune system. based on previously generated atlantic cod (gadus morhua) expressed sequence tags (ests), we identified sequences representing two paralogous amp-like transcripts. these atlantic cod paralogues were termed gaduscidins (gad-1 and gad-2), derived from the genus name gadus. we obtained full-length cdna sequences for these putative amp-encoding transcripts using bi- ... | 2010 | 21108964 |
| characterization and functional analysis of goldfish (carassius auratus l.) interleukin-10. | this is the first report of comprehensive functional analysis of an interleukin-10 in bony fish. quantitative expression analysis of goldfish il-10 revealed the greatest mrna levels in the spleen tissues, peripheral blood leukocytes and granulocytes. the stimulation of cells with recombinant goldfish (rg) tnfα2 significantly reduced il-10 mrna levels in granulocytes and monocytes of the goldfish. to functionally assess the goldfish il-10, we generated a recombinant form of the molecule (rgil-10) ... | 2010 | 21111485 |
| development and experimental validation of a 20k atlantic cod (gadus morhua) oligonucleotide microarray based on a collection of over 150,000 ests. | the collapse of atlantic cod (gadus morhua) wild populations strongly impacted the atlantic cod fishery and led to the development of cod aquaculture. in order to improve aquaculture and broodstock quality, we need to gain knowledge of genes and pathways involved in atlantic cod responses to pathogens and other stressors. the atlantic cod genomics and broodstock development project has generated over 150,000 expressed sequence tags from 42 cdna libraries representing various tissues, development ... | 2010 | 21127932 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| the rarely reported tet(31) tetracycline resistance determinant is common in gallibacterium anatis. | the present investigation was undertaken to identify and characterize the tetracycline resistance determinant in 22 gallibacterium anatis strains for which no determinant was identified using primers specific for tet(a, b, c, d, e, g, h, k, l, m, o). a recent study found tet(b) to be the most prevalent tetracycline resistance determinant in a larger collection of g. anatis field strains from mexico and denmark. however, in 41% of the tetracycline resistant strains no determinant could be assigne ... | 2010 | 21145184 |
| discovery of serum l-amino acid oxidase in the rockfish sebastes schlegeli: isolation and biochemical characterization. | fish have a complex innate defense mechanism against microbial invasion. in particular, epidermal mucus and serum in fish play important roles in innate immunity and contain a variety of bioactive substances such as complements, lectins and lysozymes, involved in host defense. recently, l-amino acid oxidases (laos) with antibacterial activity were isolated from the skin and/or gill mucous secretions of rockfish, great sculpin and flounder, and were identified to be a novel type of antibacterial ... | 2010 | 20728563 |
| genetic insertions and diversification of the polb-type dna polymerase (gp43) of t4-related phages. | in escherichia coli phage t4 and many of its phylogenetic relatives, gene 43 consists of a single cistron that encodes a polb family (polb-type) dna polymerase. we describe the divergence of this phage gene and its protein product (gp43) (gene product 43) among 26 phylogenetic relatives of t4 and discuss our observations in the context of diversity among the widely distributed polb enzymes in nature. in two t4 relatives that grow in aeromonas salmonicida phages 44rr and 25, gene 43 is fragmented ... | 2010 | 19896487 |
| design and performance of a turbot (scophthalmus maximus) oligo-microarray based on ests from immune tissues. | an expressed sequence tag database from immune tissues was used to design the first high-density turbot (scophthalmus maximus) oligo-microarray with the aim of identifying candidate genes for tolerance to pathogens. specific oligonucleotides (60 mers) were successfully designed for 2,716 out of 3,482 unique sequences of the database. an agilent custom oligo-microarray 8 x 15 k (five replicates/gene; eight microarrays/slide) was constructed. the performance of the microarray and the sources of va ... | 2010 | 19844759 |
| responses of atlantic cod gadus morhua head kidney leukocytes to phytase produced by gastrointestinal-derived bacteria. | this study identified phytase-producing bacteria that were previously isolated from the gastrointestinal tract of atlantic cod, gadus morhua and determined its effect on head kidney leukocytes. out of the 216 bacterial strains tested, the two phytase producers were identified as pseudomonas sp. and psychrobacter sp. based on their 16s rdna sequence. crude phytase from these two bacterial strains was produced employing the shake flask method. even though the total protein of the crude phytase was ... | 2010 | 19844802 |
| [current approaches to immunological diagnosis of autoimmune and immune complex diseases]. | 2010 | 2974642 | |
| mechanisms of quinolone resistance in aeromonas species isolated from humans, water and eels. | mechanisms of resistance were determined in 33 quinolone-resistant isolates of the species aeromonas hydrophila, aeromonas caviae, aeromonas media, aeromonas salmonicida, aeromonas popoffii and aeromonas veronii, recovered from humans, freshwater and eels. the quinolone resistance-determining regions (qrdrs) of gyra and parc genes were sequenced in these resistant strains, as well as in 8 quinolone-sensitive aeromonas used as controls. all quinolone-resistant aeromonas carried point mutations in ... | 2010 | 19900538 |
| characterization and expression analyses of anti-apoptotic bcl-2-like genes nr-13, mcl-1, bcl-x1, and bcl-x2 in atlantic cod (gadus morhua). | nr-13, mcl-1, and bcl-x(l), are conserved anti-apoptotic proteins that belong to the anti-apoptotic bcl-2 sub-family, which inhibits cell death by preventing mitochondrial membrane permeabilization (mmp). given the anti-apoptotic functions of these proteins in vertebrates (e.g. human, mouse, and zebrafish) and the involvement of apoptotic regulation in immune responses, we studied the sequences of these genes and their transcript expression in atlantic cod (gadus morhua) during innate immune res ... | 2010 | 19923001 |
| multiplex pcr method for detection of three aeromonas enterotoxin genes. | a novel multiplex pcr method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in aeromonas spp. this assay was used to characterize 35 reference strains as well as 537 food-borne isolates. a total of seven gene pattern combinations were encountered, including act, alt, act/alt, act/alt/ast, act/alt/148-bp amplicon, alt/ast, and alt/148-bp amplicon. the alt gene was detected with 34 reference st ... | 2010 | 19933350 |
| glycan flexibility: insights into nanosecond dynamics from a microsecond molecular dynamics simulation explaining an unusual nuclear overhauser effect. | an atomistic all-atom molecular dynamics simulation of the trisaccharide beta-d-manpnac-(1-->4)[alpha-d-glcp-(1-->3)]-alpha-l-rhap-ome with explicit solvent molecules has been carried out. the trisaccharide represents a model for the branching region of the o-chain polysaccharide of a strain from aeromonas salmonicida. the extensive md simulations having a 1-micros duration revealed a conformational dynamics process on the nanosecond time scale, that is, a 'time window' not extensively investiga ... | 2010 | 19962132 |
| the reference strain aeromonas hydrophicla cip 57.50 should be reclassified as aeromonas salmonicida cip 57.50. | the use of reference strains is a critical element for the quality control of different assays, from the development of molecular methods to the evaluation of antimicrobial activities. most of the strains used in these assays are not type strains and some of them are cited erroneously because of subsequent reclassifications and descriptions of novel species. in this study, we propose that the reference strain aeromonas hydrophila cip 57.50 be reclassified as aeromonas salmonicida cip 57.50 based ... | 2010 | 19965992 |
| identification of a novel allele of peroxisome proliferator-activated receptor gamma (pparg) and its association with resistance to aeromonas salmonicida in atlantic salmon (salmo salar). | bacterial and viral diseases are major problems in atlantic salmon aquaculture, but may be challenged through selection of brood stock with enhanced survival to diseases. today's selection strategy is based on controlled challenge tests using siblings of the breeding candidates, and is thus indirect. direct trait records on breeding candidates can potentially be provided through identification of genetic variation linked to the susceptibility to the disease. peroxisome proliferator-activated rec ... | 2010 | 20004720 |
| concurrent injection of a rhabdovirus-specific dna vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in atlantic salmon, salmo salar l. | vaccines are commonly used in salmonid aquaculture as a method of disease prevention. although there is a substantial amount of published research regarding the immunological and physiological effects following the injection of different polyvalent vaccines and dna vaccines, there are no published reports examining the physiological and immunological effects of concurrent vaccine injection, which is the situation encountered in aquaculture. using key immunological parameters such as lysozyme act ... | 2010 | 20045062 |
| in vivo transfer of plasmid pras1 between aeromonas salmonicida and aeromonas hydrophila in artificially infected cyprinus carpio l. | this study investigated the possible in vivo transfer of plasmid pras1 between aeromonas salmonicida and a. hydrophila inhabiting two different organs of cyprinus carpio l. to distinguish transconjugants from naturally occurring antibiotic resistant bacteria, twelve luminescent transposon-tagged a. hydrophila strains using mini tn5luxcdabekm2 transposon were generated. in conjugal transfer experiments, fish were conditioned with the donor bacteria and subsequently immersed in water containing th ... | 2010 | 20059637 |
| first description of atypical furunculosis in freshwater farmed atlantic salmon, salmo salar l., in chile. | we report the first isolation, identification and characterization of a group of chilean strains of atypical aeromonas salmonicida isolated from freshwater farmed atlantic salmon, salmo salar. affected fish showed superficial ulcers and pale liver with or without petechial haemorrhages. outbreaks of the disease occurred in two farms in the south of chile about 2200 km apart. five strains were isolated in pure culture and identified by serological assays and immunofluorescence tests as belonging ... | 2010 | 20298453 |
| unmethylated cpg oligodeoxynucleotides activate head kidney leukocytes of atlantic cod, gadus morhua. | bacterial dna and synthetic oligodeoxynucleotides (odns) that contain unmethylated cpg motifs are strong inducers of immune response in most mammalian organisms. the use of these synthetic cpg motifs in fish, particularly in salmonids and carp, resulted in the modulation of their immune system. however, much less is known in other species of fish such as gadoids including atlantic cod, gadus morhua. using head kidney (hk) leukocytes of cod in an in vitro study, we determined the effects of some ... | 2010 | 20349339 |
| characterization of susceptibility and carrier status of burbot, lota lota (l.), to ihnv, ipnv, flavobacterium psychrophilum, aeromonas salmonicida and renibacterium salmoninarum. | in this study, susceptibility and potential carrier status of burbot, lota lota, were assessed for five important fish pathogens. burbot demonstrated susceptibility and elevated mortality following challenge with infectious haematopoietic necrosis virus (ihnv) by immersion and to aeromonas salmonicida by intraperitoneal (i.p.) injection. ihnv persisted in fish for at least 28 days, whereas a. salmonicida was not re-isolated beyond 17 days post-challenge. in contrast, burbot appeared refractory t ... | 2010 | 20367740 |
| aeromonas salmonicida type i pilus system contributes to host colonization but not invasion. | the host-adherence strategies employed by aeromonas salmonicida subsp, salmonicida, the etiological agent of an infectious bacteremia of salmonids, are poorly understood. in addition to the outer protein coat or s-layer, a. salmonicida has both type i and type iv pili loci. the a. salmonicida type i or fim pilus is encoded by an operon with genes for a chaperone, an usher, and 3 pilus subunits and is predicted to be similar to the pap fimbriae of uropathogenic escherichia coli, which are conside ... | 2010 | 20377009 |
| atlantic cod (gadus morhua) cc chemokines: diversity and expression analysis. | chemokines are a large, diverse group of small cytokines that can be classified into several families, including the cc chemokines that are characterized by two adjacent cysteines near their amino terminus. cc chemokines play a pivotal role in host defense mechanisms by inducing leukocyte chemotaxis under physiological and inflammatory conditions. analysis of cc chemokines from teleost fishes indicates that the number of cc chemokine genes and their tissue expression patterns vary largely in thi ... | 2010 | 20381521 |
| infection-induced changes in expression of antibacterial and cytokine genes in the gill epithelial cells of atlantic cod, gadus morhua during incubation with bacterial pathogens. | the molecular processes of immune responses in mucosal tissues, such as the gills, during infection with bacterial pathogens are poorly understood. in the present study, we analyzed the transcriptional profiles of selected antibacterial genes and cytokines in the gills of a cold-water fish, atlantic cod, gadus morhua following in vitro infection with bacterial pathogens, vibrio anguillarum and atypical aeromonas salmonicida using semi-quantitative rt-pcr. there was significant upregulation in th ... | 2010 | 20430108 |
| impact of reattaching various aeromonas salmonicida a-layer proteins on vaccine efficacy in atlantic cod (gadus morhua). | atypical aeromonas salmonicida causes atypical furunculosis in a whole range of farmed fish species. the bacterium comprises a heterogeneous group differing in surface components such as the a-layer protein and o-chain polysaccharide structures. previously, the a-layer protein was shown to contribute to protective immunity as a vaccine with a. salmonicida cells with reattached a-layer protein protecting significantly better than the corresponding a-layer deficient bacteria used in the atlantic c ... | 2010 | 20472024 |
| high gene expression of inflammatory markers and il-17a correlates with severity of injection site reactions of atlantic salmon vaccinated with oil-adjuvanted vaccines. | two decades after the introduction of oil-based vaccines in the control of bacterial and viral diseases in farmed salmonids, the mechanisms of induced side effects manifested as intra-abdominal granulomas remain unresolved. side effects have been associated with generation of auto-antibodies and autoimmunity but the underlying profile of inflammatory and immune response has not been characterized. this study was undertaken with the aim to elucidate the inflammatory and immune mechanisms of granu ... | 2010 | 20507624 |
| defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions. | a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ... | 2010 | 20537393 |
| development and evaluation of a multiplex pcr assay for simultaneous detection of flavobacterium psychrophilum, yersinia ruckeri and aeromonas salmonicida subsp. salmonicida in culture fisheries. | bacterial cold water disease, enteric red mouth disease and frunculosis are the common bacterial diseases of fish worldwide. the etiologic agents of these diseases are flavobacterium (f.) psychrophilum, yersinia (y.) ruckeri and aeromonas (a.) salmonicida subsp. salmonicida, respectively. in this study, a multiplex polymerase chain reaction (m-pcr) method with yer8/10-fer3/4-fp1/3 primer pairs which can identify these fish pathogens simultaneously was developed and optimized. in optimized condit ... | 2010 | 20706031 |
| minimal incision techniques for digital deformities. | we have presented basic minimal incision approaches to the more common digital deformities. we feel that with proper evaluation of the deformity and the patient, minimal incision surgery techniques give excellent long-term results with several advantages. these advantages include: less discomfort due to less surgical dissection. less need for internal fixation by careful use of compression dressings in the immediate postoperative period along with early ambulation. less disability. compression d ... | 2010 | 2937517 |
| the antihypertensive effect of angiotensin-converting enzyme inhibition: a multifaceted mechanism of action. | 2010 | 3030977 | |
| the t cell system in rheumatoid arthritis: activated or defective? | rheumatoid arthritis (ra) is characterised by the presence of activated lymphocytes in the synovial compartment, which are classically considered to be of particular importance to the pathogenesis of the disease. we have shown that activated lymphocytes are also found in the rheumatoid lymph nodes and peripheral blood, and that their proportions are increased in early or active disease. double-labelling experiments showed that t cell subsets within the activated circulating lymphocytes resemble ... | 2010 | 3075073 |
| technique for measurement of one-dimensional instantaneous ablation velocity. | an oscillating mirror scanned a he-ne laser beam along the length of a thin rod while the rod was ablated by an argon laser beam. the shadow of the scanning beam on an image plane provides a picture of the instantaneous ablation velocity of the rod. this configuration provides a unique method for investigating one-dimensional ablation. | 2010 | 2976448 |
| congenital adrenal hyperplasia. | 2010 | 2887945 | |
| gender differences in variables associated with psychosocial adjustment to a burn injury. | the purpose of this study was to determine whether variables associated with psychosocial adjustment to a burn injury vary by gender. male and female burned subjects (n = 260) were compared on their functional disability, disfigurement, coping responses, social resources, and psychosocial adjustment to a burn injury. both men and women had adjusted psychosocially to their burn injury. less functional disability (r = .57, p less than .001) for men and greater problem-solving (r = .57, p less than ... | 2010 | 2964678 |
| [changes in several indices of the hemostatic system in patients with genital cancer during the postoperative period]. | 2010 | 2935038 | |
| evaluation of infant feeding in acute gastroenteritis. | sixty-eight bottle-fed babies under 9 months of age with mild acute gastroenteritis were observed to evaluate current feeding regimens following acute gastroenteritis in infancy. all babies were fed for 24 h with a glucose-electrolyte mixture (gem) and then randomly assigned to either a gradual reintroduction to their normal milk, i.e., slow regrade; immediate return to full-strength formula; or a rapid regrade to a hypoallergenic whey hydrolysate formula. all groups were well matched for age, s ... | 2009 | 2709254 |
| [a dynamic model of the interaction of cones with bright horizontal cells in the carp retina]. | the dynamic model showing interaction between cones and l-type horizontal cells in the carp retina is discussed. the model is based on the intracellular recording of spectral responses of horizontal cells. the model equations describe the contribution of ionic channels to responses of cones and horizontal cells. the model permits predicting responses of photopic l-cells as a function of time. | 2009 | 2812137 |
| [a dynamic model of the interaction of cones with bright horizontal cells in the carp retina]. | the dynamic model showing interaction between cones and l-type horizontal cells in the carp retina is discussed. the model is based on the intracellular recording of spectral responses of horizontal cells. the model equations describe the contribution of ionic channels to responses of cones and horizontal cells. the model permits predicting responses of photopic l-cells as a function of time. | 2009 | 2812137 |
| [frequent diagnostic errors in diseases with exanthema]. | 2009 | 2690505 | |
| greed in healthcare. | 2009 | 2798274 | |
| greed in healthcare. | 2009 | 2798274 | |
| "a few bad apples?". | 2009 | 2812134 | |
| "a few bad apples?". | 2009 | 2812134 | |
| unexpected sex-ratios in families of language/learning-impaired children. | there is a well-documented propensity of males affected with developmental language/learning impairment. results from this study demonstrate, unexpectedly, that this sex-ratio difference of males to females with developmental language/learning disorders was found to occur significantly only in families with a language/learning-impaired mother. in addition, a remarkably aberrant offspring sex-ratio was found in families of language/learning-impaired children who had an affected mother, but not fa ... | 2009 | 2771037 |
| response properties of normal observers and patients during automated perimetry. | automated perimetry was performed on both eyes of 54 normal subjects, 36 patients with ocular hypertension and normal visual fields, and 20 patients with early glaucomatous visual field loss to evaluate false-positive errors, false-negative errors, fixation losses, consistency of double determinations, and testing time. for all subject groups and response measures, large interindividual variation was found. no meaningful age-related changes were obtained for false-negative errors, false-positive ... | 2009 | 2818281 |
| response properties of normal observers and patients during automated perimetry. | automated perimetry was performed on both eyes of 54 normal subjects, 36 patients with ocular hypertension and normal visual fields, and 20 patients with early glaucomatous visual field loss to evaluate false-positive errors, false-negative errors, fixation losses, consistency of double determinations, and testing time. for all subject groups and response measures, large interindividual variation was found. no meaningful age-related changes were obtained for false-negative errors, false-positive ... | 2009 | 2818281 |
| uncertainty and visual strategies in table tennis. | ball games are characterized by perceptual uncertainty and time-pressure. of interest is the visual-search pattern carried out when these characteristics change. this is the case in table-tennis situations when comparing a drill situation, when one kind of stroke is constantly repeated, and a match situation, when the stroke is more often unpredictable. we analysed, during play, the visual-search pattern of five expert table tennis players to examine the effect of uncertainty on visual behavior. ... | 2009 | 2717357 |
| [chorioadenoma destruens. presentation of a case and review of the literature]. | 2009 | 2856452 | |
| coronary angioplasty in myocardial infarction. | 2009 | 2933946 | |
| the search for the causes of breast and colon cancer. | epidemiological studies of breast and colon cancers implicate diet as a causative factor but the evidence is stronger for colon cancer, the occurrence of which may be reduced by diets with less animal fat and more fruit and vegetables. | 2009 | 2648156 |