Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| yersinia ironomics: comparison of iron transporters among yersinia pestis biotypes and its nearest neighbor, yersinia pseudotuberculosis. | although yersinia pestis epidemic biovars and yersinia pseudotuberculosis are recently diverged, highly related species, they cause different diseases via disparate transmission routes. since iron transport systems are important for iron acquisition from hosts and for survival in the environment, we have analyzed potential iron transport systems encoded by epidemic and non-epidemic or endemic strains of y. pestis as well as two virulent y. pseudotuberculosis strains. computational biology analys ... | 2010 | 20049509 |
| the effects of substituted cyclodextrins on the colloidal and conformational stability of selected proteins. | the effects of various types of substituted and nonsubstituted cyclodextrins (cds) on the physical and colloidal stability of three different proteins were studied to further ascertain the mechanism by which cyclodextrins stabilize proteins. the three proteins examined in this study are the clostridium difficile toxoid a, yersinia pestis low-calcium-response v or v antigen (lcrv), and fibroblast growth factor 10 (fgf-10). these three pharmaceutically relevant proteins differ in molecular weight, ... | 2010 | 20049940 |
| bmper is upregulated by statins and modulates endothelial inflammation by intercellular adhesion molecule-1. | in addition to lowering cholesterol, statins exert pleiotropic effects on endothelial cells. bone morphogenetic proteins (bmps) have recently been implicated in vascular inflammation and disease. we set out to investigate the effect of statins on bmp endothelial cell precursor-derived regulator (bmper), a novel member of the bmp pathway. | 2010 | 20042706 |
| development of multitarget real-time pcr for the rapid, specific, and sensitive detection of yersinia pestis in milk and ground beef. | real-time pcr has been used previously to detect yersinia pestis; this study applies this rapid, specific, and sensitive nucleic acid-based method to the detection and quantitation of y. pestis specifically in food. five sets of primers and corresponding taqman dual-labelled fluorogenic hybridization probes for y. pestis were designed and optimized for specificity testing using genomic dna from 71 bacterial strains. four y. pestis -specific primer and probe sets were developed, based on the viru ... | 2010 | 20051199 |
| yersinia enterocolitica in slaughter pig tonsils: enumeration and detection by enrichment versus direct plating culture. | tonsil samples from 139 slaughter pigs were examined for the presence of pathogenic yersinia enterocolitica by enrichment procedures based on the standard method iso 10273:2003. in addition, samples were tested by direct plating method to evaluate its efficiency compared to the enrichment culture methods and to quantify the level of contamination in porcine tonsils. in total, 52 samples (37.4%) were positive for pathogenic y. enterocolitica, all belonging to bioserotype 4/o:3. fifty out of the 5 ... | 2010 | 19913707 |
| neonatal mucosal immunization with a non-living, non-genetically modified lactococcus lactis vaccine carrier induces systemic and local th1-type immunity and protects against lethal bacterial infection. | safe and effective immunization of newborns and infants can significantly reduce childhood mortality, yet conventional vaccines have been largely unsuccessful in stimulating the neonatal immune system. we explored the capacity of a novel mucosal antigen delivery system consisting of non-living, non-genetically modified lactococcus lactis particles, designated as gram-positive enhancer matrix (gem), to induce immune responses in the neonatal setting. yersinia pestis lcrv, used as model protective ... | 2010 | 19924118 |
| antilisterial activity of a broad-spectrum bacteriocin, enterocin lr/6 from enterococcus faecium lr/6. | enterocin lr/6, a purified bacteriocin, exhibited broad inhibitory spectrum both against related as well as some food-borne pathogens such as listeria monocytogenes, yersinia enterocolitica, aeromonas sp., shigella sp., and bacillus licheniformis. in this investigation, we have focused on l. monocytogenes as the target organism, as it is not only an important pathogen but can also survive over a wide range of environmental conditions such as refrigeration temperature, low ph, and high-salt conce ... | 2010 | 19957209 |
| quantitative anti-f1 and anti-v igg elisas as serological correlates of protection against plague in female swiss webster mice. | a recombinant fusion protein composed of yersinia pestis fraction 1 capsule (f1) and virulence-associated v antigen (v) (f1-v) has been developed as the next-generation vaccine against plague. in this study, female swiss webster mice received a single intramuscular vaccination with one of eight doses of the f1-v vaccine and exposed 4 weeks later to either y. pestis co92 or c12 organisms by the subcutaneous or aerosol routes of infection. quantitative anti-f1 and anti-v immunoglobulin g (igg) eli ... | 2010 | 19925906 |
| prevalence of yersinia enterocolitica shedding and bioserotype distribution in ontario finisher pig herds in 2001, 2002, and 2004. | we investigated characteristics of yersinia enterocolitica infection in ontario finisher pig herds. our specific objectives were to estimate or test: prevalence of y. enterocolitica shedding in finisher pigs, bioserotype distribution, agreement between the herd-level tests based on sampling pig and pooled fecal samples, whether bioserotypes cluster by farms, and whether y. enterocolitica-positive herds cluster spatially. in total, 3747 fecal samples were collected from 100 farms over the years 2 ... | 2010 | 19926152 |
| electrochemical dna biosensor based on chitosan/nano-v2o5/mwcnts composite film modified carbon ionic liquid electrode and its application to the lamp product of yersinia enterocolitica gene sequence. | a new electrochemical dna biosensor was fabricated by using a v(2)o(5) nanobelts (nano-v(2)o(5)), multi-walled carbon nanotubes (mwcnts) and chitosan (cts) nanocomposite materials modified carbon ionic liquid electrode (cile) as the working electrode. the cile was prepared by using n-hexylpyridinium hexafluorophosphate (hppf(6)) as the binder with the graphite powder. the cts-v(2)o(5)-mwcnts/cile was used as the basal electrode for the immobilization of the single-stranded dna (ssdna) probe. aft ... | 2010 | 19926468 |
| sequestering of rac by the yersinia effector yopo blocks fcgamma receptor-mediated phagocytosis. | pathogenic yersinia species neutralize innate immune mechanisms by injecting type three secretion effectors into immune cells, altering cell signaling. our study elucidates how one of these effectors, yopo, blocks phagocytosis. we demonstrate using different phagocytic models that yopo specifically blocks rac-dependent fcgamma receptor internalization pathway but not complement receptor 3-dependent uptake, which is controlled by rho activity. we show that yopo prevents rac activation but does no ... | 2010 | 19926792 |
| prevention of bubonic and pneumonic plague using plant-derived vaccines. | yersinia pestis, the causative agent of bubonic and pneumonic plague, is an extremely virulent bacterium but there are currently no approved vaccines for protection against this organism. plants represent an economical and safer alternative to fermentation-based expression systems for the production of therapeutic proteins. the recombinant plague vaccine candidates produced in plants are based on the two most immunogenic antigens of y. pestis: the fraction-1 capsular antigen (f1) and the low cal ... | 2010 | 19931370 |
| yersinia pestis two-component gene regulatory systems promote survival in human neutrophils. | human polymorphonuclear leukocytes (pmns, or neutrophils) are the most abundant innate immune cell and kill most invading bacteria through combined activities of reactive oxygen species (ros) and antimicrobial granule constituents. pathogens such as yersinia pestis resist destruction by the innate immune system and are able to survive in macrophages and neutrophils. the specific molecular mechanisms used by y. pestis to survive following phagocytosis by human pmns are incompletely defined. to ga ... | 2010 | 19933831 |
| new multispecific array as a tool for electrochemical impedance spectroscopy-based biosensing. | using electrochemical impedance spectroscopy (eis) for biosensing applications typically requires repetitive experiments. to address this need, we have designed a multispecific electrochemical array with eight individually addressable 2mm-diameter gold working electrodes for rapid biosensing data accumulation by eis in the presence of redox agent. the array allows to incorporate multiple negative controls in the course of a single binding experiment, as well as to perform parallel identical expe ... | 2010 | 19939660 |
| long-term observation of subunit vaccine f1-rv270 against yersinia pestis in mice. | long-term protection and antibody response for the subunit vaccine f1-rv270 were determined by using the mouse model. antibodies to f1 and rv270 were still detectable over a period of 518 days. the complete protection against lethal challenge of yersinia pestis could be achieved up to day 518 after primary immunization. | 2010 | 19940042 |
| a novel economic method for high throughput production of recombinant baculovirus by infecting insect cells with bacmid-containing diminopimelate-auxotrophic escherichia coli. | developing cost-effective methods for high throughput production of recombinant baculoviruses in insect cells is very challenging, because the baculovirus dna preparation and the following transfection procedure are labour-intensive and time consuming. we developed a new method of introducing recombinant bacmid dna from bacteria into insect cells simply using invasive diaminopimelate (dap) auxotrophic escherichia coli to infectspodoptera frugiperda 9 cells. the e. coli cells with recombinant bac ... | 2010 | 19835917 |
| amino acid and structural variability of yersinia pestis lcrv protein. | the lcrv protein is a multifunctional virulence factor and protective antigen of the plague bacterium and is generally conserved between the epidemic strains of yersinia pestis. we investigated the diversity in the lcrv sequences among non-epidemic y. pestis strains which have a limited virulence in selected animal models and for humans. sequencing of lcrv genes from 19 y. pestis strains belonging to different phylogenetic groups (subspecies) showed that the lcrv proteins possess four major vari ... | 2010 | 19835996 |
| effect of fat in ground beef on the growth and virulence plasmid (pyv) stability in yersinia pestis. | knowledge of the behavior of yersinia pestis in food may be useful in the event y. pestis is used in a bioterrorism attack on the food supply. however, there are no reports on the growth of plasmid-bearing (pyv) virulent y. pestis in food. the growth of a conditionally virulent pyv-bearing y. pestis kim5 in sterile raw ground beef with 7, 15 and 25% fat content was studied at 0, 4, 10 and 25 degrees c. the y. pestis kim5 did not grow but survived in raw ground beef at 0 and 4 degrees c. in raw g ... | 2010 | 19875186 |
| in vitro markers for virulence in yersinia ruckeri. | in this study, different traits that have been associated with bacterial virulence were studied in yersinia ruckeri. two isolates that had been shown to cause disease and mortality in experimentally infected rainbow trout were compared with five avirulent isolates. both virulent isolates showed high adhesion to gill and intestinal mucus of rainbow trout, whereas the majority of non-virulent strains demonstrated significantly lower adhesion. a decrease in adherence capability following bacterial ... | 2010 | 19878528 |
| sdia bridges chemical signaling between salmonella enterica serovar typhimurium and yersinia enterocolitica in mice. | 2010 | 19880607 | |
| acute toscana virus infection mimicked by yersinia-induced reactive arthritis syndrome after journey to spain. | 2010 | 19889574 | |
| immunomodulatory effects of yersinia pestis lipopolysaccharides on human macrophages. | in the current study, we investigated the activity of lipopolysaccharide (lps) purified from yersinia pestis grown at either 27 degrees c or 37 degrees c (termed lps-27 and lps-37, respectively). lps-27 containing hexa-acylated lipid a, similar to the lps present in usual gram-negative bacteria, stimulated an inflammatory response in human u937 cells through toll-like receptor 4 (tlr4). lps-37, which did not contain hexa-acylated lipid a, exhibited strong antagonistic activity to the tlr4-mediat ... | 2010 | 19889939 |
| wild boars as an important reservoir for foodborne pathogens. | one hundred fifty-three wild boars shot in the canton of geneva, switzerland, were studied for the occurrence of foodborne pathogens. tonsils and fecal samples of the animals were examined using real-time polymerase chain reaction, enzyme-linked fluorescent immunoassay, and cultural methods. the detection rate of salmonella spp., yersinia enterocolitica, yersinia pseudotuberculosis, stx-positive escherichia coli, and listeria monocytogenes was 12%, 35%, 20%, 9%, and 17%, respectively, when tonsi ... | 2010 | 19899962 |
| cellular components of probiotics control yersinia ruckeri infection in rainbow trout, oncorhynchus mykiss (walbaum). | subcellular components of the probiotics aeromonas sobria gc2 and bacillus subtilis jb-1, when administered to rainbow trout, oncorhynchus mykiss, conferred protection against a new biogroup of yersinia ruckeri. thus, intraperitoneal or intramuscular injection of rainbow trout with cell wall proteins (cwps), outer membrane proteins (omps), lipopolysaccharides (lps), whole cell proteins (wcps) and live cells followed by challenge on day 8 with y. ruckeri led to 80-100% survival compared with 10% ... | 2010 | 19912460 |
| cellular fatty acids as chemical markers for differentiation of yersinia pestis and yersinia pseudotuberculosis. | gas chromatography (gc) was utilized to investigate the cellular fatty acids (cfas) composition of 141 yersinia pestis isolates from different plague foci of china, and 20 yersinia pseudotuberculosis strains as well. | 2010 | 19912526 |
| immunomodulation using genetically engineered bacteria for type iii-mediated delivery of heterologous antigens and cytokines: potential application in vaccine and therapeutical developments. | the rational design of new approaches aiming to improve antigen or drug delivery remains a major challenge in numerous vaccine and therapeutical strategies. studies on genetically engineered bacteria have led to significant progress in the delivery of heterologous proteins. yet, the efficiency of such recombinant bacteria relies particularly on their ability to deliver sufficient amount of the drug to intended cell tissues. gram-negative bacteria like salmonella, shigella, yersinia and pseudomon ... | 2010 | 19778176 |
| dna adenine methylase is involved in the pathogenesis of edwardsiella tarda. | edwardsiella tarda is a serious aquaculture pathogen that can infect many cultured fish species. the aim of this study was to investigate the potential importance of dna adenine methylase (dam) in e. tarda pathogenesis. the e. tarda dam gene (dam(et)) was cloned from a pathogenic strain, txd1, isolated from diseased fish. dam(et) shares high (70.2%) sequence identity with the dam proteins of yersinia enterocolitica and several other bacterial species. recombinant dam(et) is able to complement a ... | 2010 | 19781866 |
| volatile antimicrobials from muscodor crispans, a novel endophytic fungus. | muscodor crispans is a recently described novel endophytic fungus of ananas ananassoides (wild pineapple) growing in the bolivian amazon basin. the fungus produces a mixture of volatile organic compounds (vocs); some of the major components of this mixture, as determined by gc/ms, are propanoic acid, 2-methyl-, methyl ester; propanoic acid, 2-methyl-; 1-butanol, 3-methyl-;1-butanol, 3-methyl-, acetate; propanoic acid, 2-methyl-, 2-methylbutyl ester; and ethanol. the fungus does not, however, pro ... | 2010 | 19797357 |
| evaluation of isolation methods for pathogenic yersinia enterocolitica from pig intestinal content. | the aim of this study was to evaluate the efficiency of four isolation methods for the detection of pathogenic yersinia enterocolitica from pig intestinal content. | 2010 | 19735331 |
| higher accumulation of f1-v fusion recombinant protein in plants after induction of protein body formation. | improving foreign protein accumulation is crucial for enhancing the commercial success of plant-based production systems since product yields have a major influence on process economics. cereal grain evolved to store large amounts of proteins in tightly organized aggregates. in maize, gamma-zein is the major storage protein synthesized by the rough endoplasmic reticulum (er) and stored in specialized organelles called protein bodies (pb). zera (gamma-zein er-accumulating domain) is the n-termina ... | 2010 | 19789982 |
| application of fluorescent nanocrystals (q-dots) for the detection of pathogenic bacteria by flow-cytometry. | fluorescent semiconductor nanocrystals (q-dots) benefit from practical features such as high fluorescence intensity, broad excitation band and emission diameter dependency. these unique spectroscopic characterizations make q-dots excellent candidates for new fluorescent labels in multi-chromatic analysis, such as flow-cytometry (fcm). in this work we shall present new possibilities of multi-labeling and multiplex analysis of pathogenic bacteria, by flow-cytometry (fcm) analysis and new specific ... | 2010 | 19826932 |
| salmonella enterica serovar typhimurium can detect acyl homoserine lactone production by yersinia enterocolitica in mice. | luxr-type transcription factors detect acyl homoserine lactones (ahls) and are typically used by bacteria to determine the population density of their own species. escherichia coli and salmonella enterica serovar typhimurium cannot synthesize ahls but can detect the ahls produced by other bacterial species using the luxr homolog, sdia. previously we determined that s. typhimurium did not detect ahls during transit through the gastrointestinal tract of a guinea pig, a rabbit, a cow, 5 mice, 6 pig ... | 2010 | 19820103 |
| loop-mediated isothermal amplification method targets to the phop gene for detection of yersinia enterocolitica. | a loop-mediated isothermal amplification (lamp) assay was developed and validated for the specific detection of yersinia enterocolitica. the assay used specifically designed primers to target within the phop gene and correctly identified all 37 strains of y. enterocolitica and 50 non-y. enterocolitica strains. the probability of detection was 100%, when the dna of extracted from 10(1) cfu y. enterocolitica was used as template in lamp assay. prior to the lamp assay, a sample preparation protocol ... | 2010 | 19833197 |
| effect of mara-like proteins on antibiotic resistance and virulence in yersinia pestis. | mara, an arac/xyls transcriptional regulator in escherichia coli, affects drug susceptibility and virulence. two mara-like proteins have been found in yersinia pestis: mara47 and mara48. deletion or overexpression of these proteins in the attenuated kim 1001 deltapgm strain led to a change in multidrug susceptibility (including susceptibility to clinically relevant drugs). additionally, lung colonization by the mara47 or mara48 deletion mutant was decreased about 10-fold in a pneumonic plague mo ... | 2010 | 19841071 |
| absence of inflammation and pneumonia during infection with nonpigmented yersinia pestis reveals a new role for the pgm locus in pathogenesis. | yersinia pestis causes primary pneumonic plague in many mammalian species, including humans, mice, and rats. virulent y. pestis strains undergo frequent spontaneous deletion of a 102-kb chromosomal dna fragment, known as the pigmentation (pgm) locus, when grown in laboratory media, yet this locus is present in every virulent isolate. the pgm locus encodes, within a high-pathogenicity island, siderophore biosynthesis genes that are required for growth in the mammalian host when inoculated by peri ... | 2010 | 19841077 |
| occurrence of salmonella, campylobacter, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes in swine. | the objective of this study was to investigate the occurrence of major bacterial foodborne pathogens in swine. in total, 359 samples from manure storage tanks (91) and fresh pooled faeces (268) obtained from finisher (110), sows (78) and weanlings (80) were collected and tested. campylobacter, salmonella, yersinia enterocolitica, escherichia coli o157 and listeria monocytogenes were isolated from 36.5%, 31.5%, 5.8%, 3.3% and 3.3% of samples respectively. all e. coli o157 isolates found on 10 far ... | 2010 | 19638163 |
| comparison of five commercial dna extraction kits for the recovery of yersinia pestis dna from bacterial suspensions and spiked environmental samples. | to evaluate commercial dna extraction kits for their ability to isolate dna from yersinia pestis suspensions and spiked environmental samples. | 2010 | 19558466 |
| spondylodiscitis of the lumbar spine in a non-immunocompromised host caused by yersinia enterocolitica o:9. | here presented is an extremely rare case of a spinal osteomyelitis (l5-s1) with epidural empyema in a non-immunocompromised 62-year-old man caused by yersinia enterocolitica o:9. the infection occurred acutely and required immediate surgical treatment. y. enterocolitica was cultured from the empyema fluid, wound swabs of the intervertebral disc l5-s1 and stool cultures. following the surgical decompression and antibiotic treatment, the patient recovered completely, without neurological deficits. ... | 2010 | 19562357 |
| deletion of braun lipoprotein gene (lpp) attenuates yersinia pestis kim/d27 strain: role of lpp in modulating host immune response, nf-kappab activation and cell death. | the pathogenic species of yersiniae potently blocks immune responses in host cells by using the type iii secretion apparatus and its effector proteins. in this study, we characterized potential mechanisms associated with the braun lipoprotein (lpp) that contributed to a further attenuation of a pigmentation locus-minus yersinia pestis kim/d27 mutant strain and its ability to generate immune responses in mice. the lpp gene encodes one of the major outer membrane lipoproteins that is involved in i ... | 2010 | 19737605 |
| heat shock proteins protect platyfish (xiphophorus maculatus) from yersinia ruckeri induced mortality. | the significant disadvantages accompanied with the use of antibiotics in aquaculture, emphasize the need for developing alternative disease control strategies, like novel vaccine approaches and immunostimulating measures. several studies have already pointed out the ability of heat shock proteins (hsps) to modulate innate and adaptive immune responses, what makes them potent candidates for the development of a new disease prevention method. in this study, the use of self and non-self heat shock ... | 2010 | 19751832 |
| cloning of a novel interleukin (il)-20-like gene in rainbow trout oncorhynchus mykiss gives an insight into the evolution of the il-10 family. | a novel il-20-like (il-20l) gene has been analyzed in rainbow trout. it has a six exon/five intron gene organisation and is translated into 181 amino acids (aa) with a signal peptide of 25aa and a mature peptide of 156aa. the trout il-20l translation shares highest identities to other il-20l molecules from fish and to il-20 in mammals. phylogenetic analysis showed that the fish il-20l molecules form an independent clade but cluster with the group containing the il-19, il-20 and il-24 molecules f ... | 2010 | 19755128 |
| transport of selected bacterial pathogens in agricultural soil and quartz sand. | the protection of groundwater supplies from microbial contamination necessitates a solid understanding of the key factors controlling the migration and retention of pathogenic organisms through the subsurface environment. the transport behavior of five waterborne pathogens was examined using laboratory-scale columns packed with clean quartz at two solution ionic strengths (10 mm and 30 mm). escherichia coli o157:h7 and yersinia enterocolitica were selected as representative gram-negative pathoge ... | 2010 | 19084252 |
| cartilage proteoglycan aggrecan epitopes induce proinflammatory autoreactive t-cell responses in rheumatoid arthritis and osteoarthritis. | to explore potential t-cell epitopes of the core protein of human cartilage proteoglycan aggrecan (pg) in patients with rheumatoid arthritis (ra) or osteoarthritis. | 2010 | 19213744 |
| cationic compounds with activity against multidrug-resistant bacteria: interest of a new compound compared with two older antiseptics, hexamidine and chlorhexidine. | use of antiseptics and disinfectants is essential in infection control practices in hospital and other healthcare settings. in this study, the in vitro activity of a new promising compound, para-guanidinoethylcalix[4]arene (cx1), has been evaluated in comparison with hexamidine (hx) and chlorhexidine (chx), two older cationic antiseptics. the mics for 69 clinical isolates comprising methicillin-resistant staphylococcus aureus, methicillin-sensitive s. aureus, coagulase-negative staphylococci (co ... | 2010 | 19456831 |
| the presence of anti-yersinia pseudotuberculosis immunoglobulins in equine serum. | the research was conducted on clinically healthy mares (n = 40) and foals (n = 78) during y. pseudotuberculosis associated enzootics. the animals were divided into groups: i to iv--mares, ia to iva--their offsprings, ib to ivb--foals which mothers were not treated with any medicaments. the animals in group i, ia and ib were injected with pbs; in group ii, iia and iib--with y. pseudotuberculosis strain-based vaccine, in group iii, iiia and iiib--with p. acnes strain-based immunostimulator; in gro ... | 2010 | 21370753 |
| [immunochemical characteristics of synthetic peptides incorporating t- and b-cell epitopes nonspecific porins of pathogenic yersinia]. | multiple antigenic peptides (maps), a sequence which include common antigenic epitopes of outer membrane porins (om) bacteria of the genus yersinia (y. pseudotuberculosis, y. enterocolitica, y. pestis), pathogenic for humans have been synthesized. after immunization of balb/c mice the antiserum to the peptide have been obtained. with the help of elisa we showed that these sera interact with porins isolated from om pathogenic yersinia, and map interact with antibodies in sera from rabbits immuniz ... | 2010 | 21317944 |
| systematic analysis of cyclic di-gmp signalling enzymes and their role in biofilm formation and virulence in yersinia pestis. | cyclic di-gmp (c-di-gmp) is a signalling molecule that governs the transition between planktonic and biofilm states. previously, we showed that the diguanylate cyclase hmst and the putative c-di-gmp phosphodiesterase hmsp inversely regulate biofilm formation through control of hmshfrs-dependent poly-β-1,6-n-acetylglucosamine synthesis. here, we systematically examine the functionality of the genes encoding putative c-di-gmp metabolic enzymes in yersinia pestis. we determine that, in addition to ... | 2010 | 21219468 |
| reduction of enteropathogenic yersinia in the pig slaughterhouse by using bagging of the rectum. | to evaluate the effectiveness of bagging of the rectum in mitigating the contamination of carcasses with enteropathogenic yersinia at the slaughterhouse and to estimate the hidden prevalences of these pathogens in different farm types and capacities, samples from pigs, carcasses, and slaughterhouse environment were collected, and a bayesian probability model was constructed. in addition, the contamination routes were studied with molecular typing of the isolated strains. according to the model, ... | 2010 | 21219732 |
| yersinia pseudotuberculosis mnth functions in intracellular manganese accumulation that is essential for virulence and survival in cells expressing functional nramp1. | manganese has an important yet undefined role in the virulence of many bacterial pathogens. in this study we confirm that a null mutation in yersinia pseudotuberculosis mnth reduces intracellular manganese accumulation. an mnth mutant was susceptible to killing by reactive oxygen species when grown under manganese limited conditions. the mnth mutant was defective in survival and growth in macrophages expressing functional nramp1, but in macrophages deficient in nramp the bacteria were able to su ... | 2010 | 21183572 |
| n15: the linear phage-plasmid. | the lambdoid phage n15 of escherichia coli is very unusual among temperate phages in that its prophage is not integrated into chromosome but is a linear plasmid molecule with covalently closed ends. upon infection the phage dna circularises via cohesive ends, then phage-encoded enzyme, protelomerase, cuts at an inverted repeat site and forms hairpin ends (telomeres) of the linear plasmid prophage. replication of the n15 prophage is initiated at an internally located ori site and proceeds bidirec ... | 2010 | 21185326 |
| effect of neem extract against the bacteria isolated from marine fish. | marine ornamental fishes are exceedingly valuable due to their high demand in domestic and international markets. there is a growing global interest to rear the fishes in captivity. but problem due to bacteria and fungi are the major hitch in captive condition. since, the use of antibiotics is banned, an attempt was made to ascertain in vitro assay of the neem leaves extract against the bacterial pathogens isolated from infected fishes. bacterial strains isolated from infected regions of the clo ... | 2010 | 21186711 |
| control of lipopolysaccharide biosynthesis by ftsh-mediated proteolysis of lpxc is conserved in enterobacteria but not in all gram-negative bacteria. | despite the essential function of lipopolysaccharides (lps) in gram-negative bacteria, it is largely unknown how the exact amount of this molecule in the outer membrane is controlled. the first committed step in lps biosynthesis is catalyzed by the lpxc enzyme. in escherichia coli, the cellular concentration of lpxc is adjusted by the only essential protease in this organism, the membrane-anchored metalloprotease ftsh. turnover of e. coli lpxc requires a length- and sequence-specific c-terminal ... | 2010 | 21193611 |
| characterization of ppcp1 plasmids in yersinia pestis strains isolated from the former soviet union. | complete sequences of 9.5-kb ppcp1 plasmids in three yersinia pestis strains from the former soviet union (fsu) were determined and compared with those of ppcp1 plasmids in three well-characterized, non-fsu y. pestis strains (kim, co92, and 91001). two of the fsu plasmids were from strains c2614 and c2944, isolated from plague foci in russia, and one plasmid was from strain c790 from kyrgyzstan. sequence analyses identified four sequence types among the six plasmids. the ppcp1 plasmids in the fs ... | 2010 | 21197443 |
| the bacterial effector cif interferes with scf ubiquitin ligase function by inhibiting deneddylation of cullin1. | cycle inhibiting factor (cif) is one of the effectors delivered into epithelial cells by enteropathogenic escherichia coli (epec) and enterohemorrhagic escherichia coli (ehec) via the type iii secretion system (ttss). cif family proteins, which inhibit host cell-cycle progression via mechanisms not yet precisely understood, are highly conserved among epec, ehec, yersinia pseudotuberculosis, photorhabdus luminescens and burkholderia pseudomallei. levels of several proteins relevant to cell-cycle ... | 2010 | 20850415 |
| a simplified method for detecting pathogenic yersinia enterocolitica in slaughtered pig tonsils. | the aim of this study was to collect preliminary data on the carriage of pathogenic yersinia enterocolitica in slaughtered pigs in france and to test a simplified method for detecting these strains from tonsils. from january to march 2009, 900 tonsil swabs were taken from pigs at one slaughterhouse in brittany, france. the swabs were vortexed in 10 ml psb broth, then 1 ml was added to 9 ml itc broth. the media were incubated for 48 h at 25°c. the psb enrichment broth was streaked on cin plates a ... | 2010 | 20850483 |
| the active site of oligogalacturonate lyase provides unique insights into cytoplasmic oligogalacturonate beta-elimination. | oligogalacturonate lyases (ogls; now also classified as pectate lyase family 22) are cytoplasmic enzymes found in pectinolytic members of enterobacteriaceae, such as the enteropathogen yersinia enterocolitica. ogls utilize a β-elimination mechanism to preferentially catalyze the conversion of saturated and unsaturated digalacturonate into monogalacturonate and the 4,5-unsaturated monogalacturonate-like molecule, 5-keto-4-deoxyuronate. to provide mechanistic insights into the specificity of this ... | 2010 | 20851883 |
| znu is the predominant zinc importer in yersinia pestis during in vitro growth but is not essential for virulence. | little is known about zn homeostasis in yersinia pestis, the plague bacillus. the znu abc transporter is essential for zinc (zn) uptake and virulence in a number of bacterial pathogens. bioinformatics analysis identified znuabc as the only apparent high-affinity zn uptake system in y. pestis. mutation of znuacb caused a growth defect in chelex-100-treated pmh2 growth medium, which was alleviated by supplementation with submicromolar concentrations of zn. use of transcriptional reporters confirme ... | 2010 | 20855510 |
| deconvolution and database search of complex tandem mass spectra of intact proteins: a combinatorial approach. | top-down proteomics studies intact proteins, enabling new opportunities for analyzing post-translational modifications. because tandem mass spectra of intact proteins are very complex, spectral deconvolution (grouping peaks into isotopomer envelopes) is a key initial stage for their interpretation. in such spectra, isotopomer envelopes of different protein fragments span overlapping regions on the m/z axis and even share spectral peaks. this raises both pattern recognition and combinatorial chal ... | 2010 | 20855543 |
| outer membrane protein x (ail) contributes to yersinia pestis virulence in pneumonic plague and its activity is dependent on the lipopolysaccharide core length. | yersinia pestis, the causative agent of plague, is one of the most virulent microorganisms known. the outer membrane protein x (ompx) in y. pestis kim is required for efficient bacterial adherence to and internalization by cultured hep-2 cells and confers resistance to human serum. here, we tested the contribution of ompx to disease progression in the fully virulent y. pestis co92 strain by engineering a deletion mutant and comparing its ability in mediating pneumonic plague to that of the wild ... | 2010 | 20837715 |
| tnfα and ifnγ contribute to f1/lcrv-targeted immune defense in mouse models of fully virulent pneumonic plague. | immunization with the yersinia pestis f1 and lcrv proteins improves survival in mouse and non-human primate models of pneumonic plague. f1- and lcrv-specific antibodies contribute to protection, however, the mechanisms of antibody-mediated defense are incompletely understood and serum antibody titers do not suffice as quantitative correlates of protection. previously we demonstrated roles for tumor necrosis factor-alpha (tnfα) and gamma-interferon (ifnγ) during defense against conditionally atte ... | 2010 | 20840834 |
| mass spectrometry biotyper system identifies enteric bacterial pathogens directly from colonies grown on selective stool culture media. | we evaluated the performance and cost-effectiveness of a matrix-assisted laser desorption ionization time-of-flight mass spectrometry-based biotyper system for the routine identification of common enteric bacterial pathogens seen in middle tennessee from suspicious colonies grown on selective stool culture media. a total of 304 suspicious colonies were selected and further identified from 605 stool specimens. the suspicious colonies were analyzed by the biotyper system, and the results were comp ... | 2010 | 20844226 |
| formulation and immunogenicity of a potential multivalent type iii secretion system-based protein vaccine. | the virulence of many pathogenic gram-negative bacteria is dependent upon their type iii secretion (tts) systems. here, we discuss initial formulation studies of five tts needle tip proteins ipad (shigella flexneri), bipd (burkholderia pseudomallei), sipd (salmonella spp.), lcrv (yersinia spp.), and pcrv (pseudomonas aeruginosa) as targets for subunit vaccines. excipient screening and subsequent assays lead to the selection of 10% sucrose and 5% dextrose as an optimal stabilizer combination for ... | 2010 | 20845449 |
| assessment of the microbiological safety of dried spices and herbs commercialized in spain. | spices and herbs are natural products or their blends that must be free of extraneous matter content. conventional production of these products implicates a number of hygienic problems so spices and herbs may be exposed to a wide range of microbial contamination during pre- and post-harvest and they can present high microbial counts. in this study, we have analyzed the microbial quality of 53 samples of spices and dry herbs collected from spanish markets detecting a contamination of samples of s ... | 2010 | 20848208 |
| reliable detection of bacillus anthracis, francisella tularensis and yersinia pestis by using multiplex qpcr including internal controls for nucleic acid extraction and amplification. | several pathogens could seriously affect public health if not recognized timely. to reduce the impact of such highly pathogenic micro-organisms, rapid and accurate diagnostic tools are needed for their detection in various samples, including environmental samples. | 2010 | 21143837 |
| distinct ccr2(+) gr1(+) cells control growth of the yersinia pestis δyopm mutant in liver and spleen during systemic plague. | we are using a systemic plague model to identify the cells and pathways that are undermined by the virulence protein yopm of the plague bacterium yersinia pestis. in this study, we pursued previous findings that gr1(+) cells are required to selectively limit growth of δyopm y. pestis and that cd11b(+) cells other than polymorphonuclear leukocytes (pmns) are selectively lost in spleens infected with parent y. pestis. when pmns were ablated from mice, δyopm y. pestis grew as well as the parent str ... | 2010 | 21149593 |
| destabilization of yope by the ubiquitin-proteasome pathway fine-tunes yop delivery into host cells and facilitates systemic spread of yersinia enterocolitica in host lymphoid tissue. | pathogenic yersinia species inject a panel of yop virulence proteins by type iii protein secretion into host cells to modulate cellular defense responses. this enables the survival and dissemination of the bacteria in the host lymphoid tissue. we have previously shown that yope of the y. enterocolitica serogroup o8 is degraded in the host cell through the ubiquitin-proteasome pathway. yope normally manipulates rearrangements of the actin cytoskeleton and triggers phagocytosis resistance. to shed ... | 2010 | 21149597 |
| intussusception caused by yersinia enterocolitica enterocolitis in a patient with sickle cell anemia. | yersinia enterocolitica intussusception is rarely encountered in patients without an underlying susceptibility and is most frequently reported in iron-overloaded patients. this is thought to be related to the unusual use of iron by this microorganism. we present a case of a 5-year old child with intussusception of the terminal ileum caused by y. enterocolitica whose past medical history was significant for sickle cell disease. this type of presentation is extremely rare. his monthly blood transf ... | 2010 | 21151549 |
| diversity of bacteria associated with bursaphelenchus xylophilus and other nematodes isolated from pinus pinaster trees with pine wilt disease. | the pinewood nematode (pwn), bursaphelenchus xylophilus, has been thought to be the only causal agent of pine wilt disease (pwd), however, since bacteria have been suggested to play a role in pwd, it is important to know the diversity of the microbial community associated to it. this study aimed to assess the microbial community associated with b. xylophilus and with other nematodes isolated from pine trees, pinus pinaster, with pwd from three different affected forest areas in portugal. one hun ... | 2010 | 21151611 |
| functional analysis of vopf activity required for colonization in vibrio cholerae. | vibrio cholerae, a gram-negative facultative pathogen, is the etiologic agent for the diarrheal disease cholera. we previously characterized a clinical isolate, am-19226, that translocates a type iii secretion system (t3ss) effector protein with actin-nucleating activity, vopf, into the host cells. from comparative genomic studies, we identified a divergent t3ss island in additional isolates which possess a vopf homolog, vopn. unlike the vopf-mediated protrusion formation, vopn localizes to stre ... | 2010 | 21151774 |
| [primary pneumonic plague with nosocomial transmission in la libertad, peru 2010]. | pneumonic plague is one of the clinical forms of plague, of low frequency and high mortality, transmitted by direct inhalation of yersinia pestis coming from an animal or from person to person. | 2010 | 21152724 |
| functional characterization of a nonmammalian il-21: rainbow trout oncorhynchus mykiss il-21 upregulates the expression of the th cell signature cytokines ifn-gamma, il-10, and il-22. | in mammals, il-21 is a common γ chain cytokine produced by activated cd4(+) t cells and nkt cells that acts on multiple lineages of cells. although il-21 has also been discovered in birds, amphibians, and fish, to date, no functional studies have been reported for any nonmammalian il-21 molecule. we have sequenced an il-21 gene (til-21) in rainbow trout, which has a six-exon/five-intron structure, is expressed in immune tissues, and is induced by bacterial and viral infection and the t cell stim ... | 2010 | 21160047 |
| a novel cdsab operon is involved in the uptake of l-cysteine and participates in the pathogenesis of yersinia ruckeri. | application of in vivo expression technology (ivet) to yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. analysis of the translational products of both genes showed permease domains (open reading frame 1 [orf1]) and amino acid position identities (orf2) with the l-cysteine desulfidase from methanocaldococcus jannaschii, a new type of enzyme involved in the br ... | 2010 | 21169490 |
| oligomeric coiled-coil adhesin yada is a double-edged sword. | yersinia adhesin a (yada) is an essential virulence factor for the food-borne pathogens yersinia enterocolitica and yersinia pseudotuberculosis. surprisingly, it is a pseudogene in yersinia pestis. even more intriguing, the introduction of a functional yada gene in y. pestis ev76 was shown to correlate with a decrease in virulence in a mouse model. here, we report that wild type (wt) y. enterocolitica e40, as well as yada-deprived e40 induced the synthesis of neutrophil extracellular traps (nets ... | 2010 | 21170337 |
| structure of the metal-dependent deacetylase lpxc from yersinia enterocolitica complexed with the potent inhibitor chir-090 . | the first committed step of lipid a biosynthesis is catalyzed by udp-(3-o-((r)-3-hydroxymyristoyl))-n-acetylglucosamine deacetylase, a metal-dependent deacetylase also known as lpxc. because lipid a is essential for bacterial viability, the inhibition of lpxc is an appealing therapeutic strategy for the treatment of gram-negative bacterial infections. here we report the 1.79 å resolution x-ray crystal structure of lpxc from yersinia enterocolitica (yelpxc) complexed with the potent hydroxamate i ... | 2010 | 21171638 |
| il-17 contributes to cell-mediated defense against pulmonary yersinia pestis infection. | pneumonic plague is one of the world's most deadly infectious diseases. the causative bacterium, yersinia pestis, has the potential to be exploited as a biological weapon, and no vaccine is available. vaccinating b cell-deficient mice with d27-plpxl, a live attenuated y. pestis strain, induces cell-mediated protection against lethal pulmonary y. pestis challenge. in this article, we demonstrate that prime/boost vaccination with d27-plpxl confers better protection than prime-only vaccination. the ... | 2010 | 21172869 |
| polymerase chain reaction (pcr) identification of rodent blood meals confirms host sharing by flea vectors of plague. | elucidating feeding relationships between hosts and parasites remains a significant challenge in studies of the ecology of infectious diseases, especially those involving small or cryptic vectors. black-tailed prairie dogs (cynomys ludovicianus) are a species of conservation importance in the north american great plains whose populations are extirpated by plague, a flea-vectored, bacterial disease. using polymerase chain reaction (pcr) assays, we determined that fleas (oropsylla hirsuta) associa ... | 2010 | 21175944 |
| dioxygenases in burkholderia ambifaria and yersinia pestis that hydroxylate the outer kdo unit of lipopolysaccharide. | several gram-negative pathogens, including yersinia pestis, burkholderia cepacia, and acinetobacter haemolyticus, synthesize an isosteric analog of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), known as d-glycero-d-talo-oct-2-ulosonic acid (ko), in which the axial hydrogen atom at the kdo 3-position is replaced with oh. here we report a unique kdo 3-hydroxylase (kdoo) from burkholderia ambifaria and yersinia pestis, encoded by the bamb_0774 (bakdoo) and the y1812 (ypkdoo) genes, respectively. when ... | 2010 | 21178073 |
| role of the yersinia pestis yersiniabactin iron acquisition system in the incidence of flea-borne plague. | plague is a flea-borne zoonosis caused by the bacterium yersinia pestis. y. pestis mutants lacking the yersiniabactin (ybt) siderophore-based iron transport system are avirulent when inoculated intradermally but fully virulent when inoculated intravenously in mice. presumably, ybt is required to provide sufficient iron at the peripheral injection site, suggesting that ybt would be an essential virulence factor for flea-borne plague. here, using a flea-to-mouse transmission model, we show that a ... | 2010 | 21179420 |
| hijacking of the pleiotropic cytokine interferon-γ by the type iii secretion system of yersinia pestis. | yersinia pestis, the causative agent of bubonic plague, employs its type iii secretion system to inject toxins into target cells, a crucial step in infection establishment. lcrv is an essential component of the t3ss of yersinia spp, and is able to associate at the tip of the secretion needle and take part in the translocation of anti-host effector proteins into the eukaryotic cell cytoplasm. upon cell contact, lcrv is also released into the surrounding medium where it has been shown to block the ... | 2010 | 21179438 |
| cell membrane is impaired, accompanied by enhanced type iii secretion system expression in yersinia pestis deficient in rova regulator. | in the enteropathogenic yersinia species, rova regulates the expression of invasin, which is important for enteropathogenic pathogenesis but is inactivated in yersinia pestis. investigation of the rova regulon in y. pestis at 26 °c has revealed that rova is a global regulator that contributes to virulence in part by the direct regulation of psaefabc. however, the regulatory roles of rova in y. pestis at 37 °c, which allows most virulence factors in mammalian hosts to be expressed, are still poor ... | 2010 | 20862262 |
| complete genome sequence of crohn's disease-associated adherent-invasive e. coli strain lf82. | ileal lesions of crohn's disease (cd) patients are abnormally colonized by pathogenic adherent-invasive escherichia coli (aiec) able to invade and to replicate within intestinal epithelial cells and macrophages. | 2010 | 20862302 |
| simultaneous pathogen detection and antibiotic resistance characterization using snp-based multiplexed oligonucleotide ligation-pcr (mol-pcr). | extensive use of antibiotics in both public health and animal husbandry has resulted in rapid emergence of antibiotic resistance in almost all human pathogens, including biothreat pathogens. antibiotic resistance has thus become a major concern for both public health and national security. we developed multiplexed assays for rapid, simultaneous pathogen detection and characterization of ciprofloxacin and doxycycline resistance in bacillus anthracis, yersinia pestis, and francisella tularensis. t ... | 2010 | 20865530 |
| docking to large allosteric binding sites on protein surfaces. | the inactive porphobilinogen synthase (pbgs) hexamer has an oligomer-specific and phylogenetically variable surface cavity that is not present in the active octamer. the octamer and hexamer are components of a dynamic quaternary structure equilibrium characteristic of morpheeins. small molecules that bind to the hexamer-specific surface cavity, which is at the interface of three subunits, are predicted to act as allosteric inhibitors that function by drawing the oligomeric equilibrium toward the ... | 2010 | 20865533 |
| characterization of yersinia using maldi-tof mass spectrometry and chemometrics. | yersinia are gram-negative, rod-shaped facultative anaerobes, and some of them, yersinia enterocolitica, yersinia pseudotuberculosis, and yersinia pestis, are pathogenic in humans. rapid and accurate identification of yersinia strains is essential for appropriate therapeutic management and timely intervention for infection control. in the past decade matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms) in combination with computer-aided pattern recognitio ... | 2010 | 20866090 |
| identification of ysrt and evidence that ysrrst constitute a unique phosphorelay system in yersinia enterocolitica. | two-component systems (tcs) and phosphorelay systems are mechanisms used by bacteria and fungi to quickly adapt to environmental changes to produce proteins necessary for survival in new environments. bacterial pathogens use tcs and phosphorelay systems to regulate genes necessary to establish infection within their hosts, including type iii secretion systems (t3ss). the yersinia enterocolitica ysa t3ss is activated in response to nacl by ysrs and ysrr, a putative hybrid sensor kinase and a resp ... | 2010 | 20870771 |
| yersinia pseudotuberculosis colitis presented with severe gastrointestinal bleeding. | we report an adult case of yersinia pseudotuberculosis colitis who presented with severe gastrointestinal bleeding. a 25-year-old male had admitted with fever, vomiting, body aches, and massive lower gastrointestinal bleeding. since diagnostic tests were unremarkable and the patient's hemodynamic condition was unstable, emergency explorative laparotomy was performed. during the operation, localized wall thickening and ulcers were seen in the cecum. right hemicolectomy was performed. histological ... | 2010 | 20872335 |
| [preparation of magnetic latexes and their use for the immunodetection of microbial antigens]. | the possibility of detecting antigens of plague, tularemia, and brucellosis microbes with magnetic latex (ml)-based test systems has been demonstrated. mls were prepared from latexes (polyacroleine microspheres, 1.2-1.8 +/- 0.1 microm) by exposing the particles to a 25-35%-solution of ferrous sulfate for 0.5 h and then to a 15-25%-aqueous solution of ammonia for 0.5 h in a 100 degrees c water bath and dehydrating after each operation. the possibility of preparing magnetic latex immunosorbents (m ... | 2010 | 20873177 |
| new rural focus of plague, algeria. | 2010 | 20875302 | |
| body lice, yersinia pestis orientalis, and black death. | 2010 | 20875308 | |
| the sequence of the pyv virulence plasmid from yersinia enterocolitica strain wa-314 biogroup 1b serotype o:8. | our laboratory strain yersinia enterocolitica strain wa-314 biogroup 1b serotype o:8 displayed a different adhesion behavior to host cells compared to other yersinia strains. to investigate whether this is based on differences in the gene content of the large pyv virulence plasmid which contains the major yersinia adhesin yada, we set out to sequence pyv(wa-314). pyv(wa-314) is very similar to pyv127/90, with a notable difference in the length of the type iii secretion system component yscp, whi ... | 2010 | 20875451 |
| development of a vaccinia virus based reservoir-targeted vaccine against yersinia pestis. | yersinia pestis, the causative organism of plague, is a zoonotic organism with a worldwide distribution. although the last plague epidemic occurred in early 1900s, human cases continue to occur due to contact with infected wild animals. in this study, we have developed a reservoir-targeted vaccine against y. pestis, to interrupt transmission of disease in wild animals as a potential strategy for decreasing human disease. a vaccinia virus delivery system was used to express the f1 capsular protei ... | 2010 | 20875494 |
| transcriptomic and innate immune responses to yersinia pestis in the lymph node during bubonic plague. | a delayed inflammatory response is a prominent feature of infection with yersinia pestis, the agent of bubonic and pneumonic plague. using a rat model of bubonic plague, we examined lymph node histopathology, transcriptome, and extracellular cytokine levels to broadly characterize the kinetics and extent of the host response to y. pestis and how it is influenced by the yersinia virulence plasmid (pyv). remarkably, dissemination and multiplication of wild-type y. pestis during the bubonic stage o ... | 2010 | 20876291 |
| alternative endogenous protein processing via an autophagy-dependent pathway compensates for yersinia-mediated inhibition of endosomal major histocompatibility complex class ii antigen presentation. | extracellular yersinia pseudotuberculosis employs a type iii secretion system (t3ss) for translocating virulence factors (yersinia outer proteins [yops]) directly into the cytosol of eukaryotic cells. recently, we used yope as a carrier molecule for t3ss-dependent secretion and translocation of listeriolysin o (llo) from listeria monocytogenes. we demonstrated that translocation of chimeric yope/llo into the cytosol of macrophages by yersinia results in the induction of a codominant antigen-spec ... | 2010 | 20876292 |
| a multi-pronged search for a common structural motif in the secretion signal of salmonella enterica serovar typhimurium type iii effector proteins. | many pathogenic gram-negative bacteria use a type iii secretion system (t3ss) to deliver effector proteins into the host cell where they reprogram host defenses and facilitate pathogenesis. the first 20-30 n-terminal residues usually contain the 'secretion signal' that targets effector proteins for translocation, however, a consensus sequence motif has never been discerned. recent machine-learning approaches, such as support vector machine (svm)-based identification and evaluation of virulence e ... | 2010 | 20877914 |
| the enigma of yersinia enterocolitica biovar 1a. | yersinia enterocolitica, an important food- and water-borne enteropathogen causes acute diarrhea, terminal ileitis, and mesenteric lymphadenitis. it is represented by six biovars (1a, 1b, 2-5). the biovar 1a strains are generally regarded as avirulent as they lack pyv plasmid and major chromosomal virulence genes. despite this, some biovar 1a strains produce disease symptoms indistinguishable from that produced by known pathogenic biovars (1b, 2-5). suggested prospective studies to understand pa ... | 2010 | 20883083 |
| erythema nodosum associated with yersinia enterocolitica infection. | we report a 74-year-old woman who presented to hospital with fever, vomiting, diarrhea, and 2 weeks later developed erythema nodosum (en) on the legs, and was diagnosed with yersinia enterocolitica infection based on her clinical course and microbiological examination of the stool. she also had a complication of pancreatitis, which made the diagnosis challenging. we should suspect infection by y. enterocolitica when diagnosing cases of en with gastrointestinal symptoms. we assume en is likely to ... | 2010 | 20883368 |
| expression, refolding, and initial structural characterization of the y. pestis ail outer membrane protein in lipids. | ail is an outer membrane protein and virulence factor of yersinia pestis, an extremely pathogenic, category a biothreat agent, responsible for precipitating massive human plague pandemics throughout history. due to its key role in bacterial adhesion to host cells and bacterial resistance to host defense, ail is a key target for anti-plague therapy. however, little information is available about the molecular aspects of its function and interactions with the human host, and the structure of ail i ... | 2010 | 20883662 |
| two stacked heme molecules in the binding pocket of the periplasmic heme-binding protein hmut from yersinia pestis. | the periplasmic binding protein hmut from yersinia pestis (yphmut) is a component of the heme uptake locus hmu and delivers bound hemin to the inner-membrane-localized, atp-binding cassette (abc) transporter hmuuv for translocation into the cytoplasm. the mechanism of this process, heme transport across the inner membrane of pathogenic bacteria, is currently insufficiently understood at the molecular level. here we describe the crystal structures of the substrate-free and heme-bound states of yp ... | 2010 | 20888343 |
| in vitro activation of murine peritoneal macrophages by recombinant yopj: production of nitric oxide, proinflammatory cytokines and chemokines. | recently it was reported that 3 μg/ml of recombinant yopj induced apoptosis in murine peritoneal macrophages in vitro. however, in this study, we report the activation of murine peritoneal macrophages in vitro on treatment with sub-apoptotic dose of recombinant yopj protein (1 μg/ml). the activation involves enhanced production of nitric oxide (no), tumor necrosis factor-α (tnf-α), il-12, and il-6. production of no and il-6 was found to peak at 24 h of ryopj treatment, whereas il-12 and ifn-γ pr ... | 2010 | 20888665 |
| range-wide determinants of plague distribution in north america. | plague, caused by the bacterium yersinia pestis, is established across western north america, and yet little is known of what determines the broad-scale dimensions of its overall range. we tested whether its north american distribution represents a composite of individual host-plague associations (the "host niche hypothesis"), or whether mammal hosts become infected only at sites overlapping ecological conditions appropriate for plague transmission and maintenance (the "plague niche hypothesis") ... | 2010 | 20889857 |