Publications

ompk26, a novel porin associated to carbapenem resistance in klebsiella pneumoniae.	clinical isolates of k. pneumoniae resistant to carbapenems are being isolated with increasing frequency. loss of the expression of the major non-specific porins ompk35/36 is a frequent feature in these isolates. in this study, we look for porins that could compensate for the loss of the major porins in carbapenem resistant organisms. comparison of the outer membrane proteins from two k. pneumoniae clinical isogenic isolates susceptible (kpcs-1) and resistant (kpcr-1) to carbapenems revealed the ...	2011	21807980
synergy of fosfomycin with carbapenems, colistin, netilmicin, and tigecycline against multidrug-resistant klebsiella pneumoniae, escherichia coli, and pseudomonas aeruginosa clinical isolates.	fosfomycin represents a potential last-resort treatment option for infections with certain multidrug-resistant (mdr) gram-negative pathogens. we evaluated double-drug combinations of fosfomycin with imipenem, meropenem, doripenem, colistin, netilmicin, and tigecycline for in vitro synergy against 100 mdr klebsiella pneumoniae, escherichia coli, and pseudomonas aeruginosa clinical isolates, using the etest method. synergy was defined as a fractional inhibitory concentration index =0.5. the isolat ...	2011	21805292
synthesis, characterization, theoretical crystal structure, and antibacterial activities of some transition metal complexes of the thiosemicarbazone (z)-2-(pyrrolidin-2-ylidene)hydrazinecarbothioamide.	problem statement. in iraq like most third world countries, attempts discovered new antibiotic drugs derived from thiosemicarbazide and its metal complexes and developed the branch of applied in organic chemistry. approach. new (z)-2-(pyrrolidin-2-ylidene)hydrazinecarbothioamide (l) was synthesized in a good yield by the reaction of pyrrolidone with thiosemicarbazide. co(ii), ni(ii), and cu(ii) complexes of (l) were prepared and characterized by ft-ir, uv/visible spectra, (1)hnmr, and chn analys ...	2011	21804771
klebsiella ozaenae cholecystitis.	the authors report a case of klebsiella ozaenae septicemia, cholecystitis and urinary tract infection in a 65-year-old morbidly obese woman with diabetes. klebsiella ozaenae is considered a nonpathogen or colonizer rarely causing septicemia. this is the first reported case of cholecystitis due to this agent.	2011	21804376
antimicrobial susceptibility patterns of clinical isolates of gram-negative bacteria obtained from intensive care units in a tertiary hospital in beijing, china.	in this study we investigated the prevalence of antimicrobial resistance in clinical isolates of gram-negative bacteria obtained from intensive care units (icus) in the people's liberation army (pla) 309 hospital located in beijing, china. between 2007 and 2010, a total of 1949 isolates of gram-negative bacteria were collected and tested using an antibiotic susceptibility assay. a marked decrease was observed in the susceptibility of acinetobacter baumannii to imipenem and amikacin as compared t ...	2011	21803697
longitudinal characterization of acinetobacter baumannii-calcoaceticus complex, klebsiella pneumoniae, and methicillin-resistant staphylococcus aureus colonizing and infecting combat casualties.	drug-resistant acinetobacter baumannii-calcoaceticus complex, klebsiella pneumoniae, and methicillin-resistant staphylococcus aureus colonize and infect combat casualties from iraq and afghanistan. we retrospectively evaluated relatedness, by pulsed-field gel electrophoresis and antibiotic susceptibility testing, of isolates colonizing and infecting casualties over 2 years. colonizing organisms were unrelated to isolates producing later infection in up to 27% of cases; most isolates underwent ch ...	2011	21802175
first isolation and outbreak of oxa-48-producing klebsiella pneumoniae in an irish hospital, march to june 2011.		2011	21801694
dna capturing machinery through spore-displayed proteins.	aims: the purpose of this study was to develop a general method for the facile development of a new dna biosensor which utilizes streptavidin-displayed spores as a molecular machinery. methods and results: fluorescence spectroscopy was used as a monitoring tool for the streptavidin displayed on the surface of bacillus thuringiensis spores and as a diagnosis method for dna detection. as a proof-of-concept, four pathogenic bacteria including pseudomonas aeruginosa, acinetobacter baumannii, esche ...	2011	21801185
effect of spacer sequences between blactx-m and isecp1 on blactx-m expression.	spacer sequences sized 42 and 127 bp, respectively, between isecp1 insertion sequence and the bla(ctx-m) were observed in klebsiella pneumoniae. however, it is not known whether different spacers in the upstream of bla(ctx-m) and a promoter within the 127 bp spacer will influence cephalosporin resistance. three recombinant plasmids with different spacers and with or without the isecp1 were constructed to compare the bla(ctx-m-3) expression and susceptibility to cephalosporins. our experiment rev ...	2011	21799195
effect of an antibiotic algorithm on the adequacy of empiric antibiotic therapy given by a medical emergency team.	introduction: delayed administration of effective antimicrobial therapy increases mortality in patients with septic shock. empiric antibiotic selection in this setting can be inaccurate. the objective of this study was to determine whether an antibiotic algorithm (aa) tailored to institutional resistance patterns improves the adequacy of antimicrobial therapy. methods: a retrospective review of our rapid response system database was performed. patients with possible sepsis with positive microbio ...	2011	21798704
spread of carbapenem-resistant klebsiella pneumoniae in a tertiary hospital in sao paulo, brazil.		2011	21798628
[expression of glycerol dehydrogenase gene in escherichia coli by codon optimization].	to improve expression level of glycerol dehydrogenase gene glda in escherichia coli by means of codon optimization.	2011	21796985
[screening and breeding high 1,3-propanediol producing strains by genome shuffling].	to improve the tolerance of main metabolites, we used genome shuffling to achieve high 1,3-propanediol producing mutants.	2011	21796981
rising bacterial resistance to common antibiotics in al ain, united arab emirates.	there is a dearth of local information in al amin, united arab emirates about antibiotic resistance patterns. in this retrospective study in a tertiary referral hospital, antibiotic susceptibility results were analysed over the 5-year period 2004-08 and compared with a previous study in the same hospital during 1999-2002. staphylococcus aureus showed a significant decrease in sensitivity to oxacillin from 95.0% in the period 1999-2002 to 84.4% in 2008. sensitivity of acinetobacter spp. to imipen ...	2011	21796964
structure of 2-oxo-3-deoxygalactonate kinase from klebsiella pneumoniae.	in most organisms, efficient d-galactose utilization requires the highly conserved leloir pathway that converts d-galactose to d-glucose 1-phosphate. however, in some bacterial and fungal species alternative routes of d-galactose assimilation have been identified. in the so-called de ley-doudoroff pathway, d-galactose is metabolized into pyruvate and d-glyceraldehyde 3-phosphate in five consecutive reactions carried out by specific enzymes. the penultimate step in this pathway involves the phosp ...	2011	21795809
structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from klebsiella pneumoniae.	the stereospecific oxidative degradation of uric acid to (s)-allantoin has recently been demonstrated to proceed via two unstable intermediates and requires three separate enzymatic reactions. the second step of this reaction, the conversion of 5-hydroxyisourate (hiu) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline, is catalyzed by hiu hydrolase (hiuh). the high-resolution crystal structure of hiuh from the opportunistic pathogen klebsiella pneumoniae (kphiuh) has been determined. kphiuh is a h ...	2011	21795808
synthesis and studies of novel 2-(4-cyano-3-trifluoromethylphenyl amino)-4-(quinoline-4-yloxy)-6-(piperazinyl/piperidinyl)-s-triazines as potential antimicrobial, antimycobacterial and anticancer agents.	a series of novel s-triazine analogs were synthesized and characterized by ir, (1)h nmr, (13)c nmr, (19)f nmr spectroscopy and elemental analysis. preliminary screening of target compounds against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberculosis ...	2011	21794959
contribution of mucoviscosity associated gene a (maga) to virulence in experimental klebsiella pneumoniae endophthalmitis.	purpose: endogenous endophthalmitis secondary to klebsiella pneumoniae liver abscess is a blinding infection that is being reported more frequently in the literature. the k1 capsule and maga contribute to virulence of systemic infection in mice, however, little is known about the role of maga in secondary ocular infections. methods: to assess the role of k. pneumoniae capsule in endophthalmitis, we induced experimental endophthalmitis by direct inoculation of 100 cfu of wild type, maga-deficient ...	2011	21791595
heat shock protein 60 from klebsiella pneumoniae protects mononuclear cells from apoptotic cell death induced by dexamethasone.	aims: bacterial heat shock proteins can have anti-apoptotic effects on human cells. we investigated whether enterobacterial hsp60 can protect peripheral blood mononuclear cells (pbmc) from dxm-induced apoptosis and if this effect requires cytoskeleton participation. main methods: anti-apoptotic effect from enterobacterial hsp60 was analyzed by adding these proteins to peripheral mononuclear cells cultures before dxm induction. percentage of apoptotic cells was determined by subg0 peak and tunel ...	2011	21791241
antibacterial efficacy of lytic bacteriophages against antibiotic-resistant klebsiella species.	bacterial resistance to antibiotics is a leading and highly prevalent problem in the treatment of infectious diseases. bacteriophages (phages) appear to be effective and safe alternatives for the treatment of resistant infections because of their specificity for bacterial species and lack of infectivity in eukaryotic cells. the present study aimed to isolate bacteriophages against klebsiella spp. and evaluate their efficacy against antibiotic-resistant species. seventy-two antibiotic-resistant k ...	2011	21789469
safety of pediatric percutaneous liver biopsy performed by interventional radiologists.	: national data suggest that pediatric percutaneous liver biopsy is increasingly being performed by interventional radiologists rather than pediatric gastroenterologists. the objective of the present report is to describe the safety and effectiveness of percutaneous liver biopsy performed by interventional radiologists in a large cohort of children and to compare the results with the existing literature on biopsies performed by pediatric gastroenterologists.	2011	21788763
further increases in carbapenem-, amikacin-, and fluoroquinolone-resistant isolates of acinetobacter spp. and p. aeruginosa in korea: konsar study 2009.	purpose: the increasing prevalence of antimicrobial resistant bacteria has become a serious worldwide problem. the aim of this study was to analyze antimicrobial resistance data generated in 2009 by hospitals and commercial laboratories participating in the korean nationwide surveillance of antimicrobial resistance program. materials and methods: susceptibility data were collected from 24 hospitals and two commercial laboratories. in the analysis, resistance did not include intermediate suscepti ...	2011	21786445
in vitro activity of 5-(2,4-dimethylbenzyl) pyrrolidin-2-one extracted from marine streptomyces vitsvk5 spp. against fungal and bacterial human pathogens.	background: pharmacological screening and usage of natural products for the treatment of human diseases has had a long history from traditional medicine to modern drugs. the majority of modern drugs are reported to be mostly from natural products. objective: the aim of the present study was to evaluate the inhibitory activity of 5-(2,4-dimethylbenzyl) pyrrolidin-2-one (dmbpo) extracted from marine streptomyces vitsvk5 spp. isolated from sediment samples collected at marakkanam coast of bay of be ...	2011	21782963
design and synthesis of spiro[indole-thiazolidine]spiro[indole-pyrans] as antimicrobial agents.	a series of novel spiro[indole-thiazolidine]spiro[indole-pyran] derivatives were synthesized from n-(bromoalkyl)indol-2,3-diones via monospiro-bisindole intermediates; the two indole nuclei being connected via n-(ch(2))(n)-n linker. synthesized compounds were evaluated for their antimicrobial activities in vitro against three gram-positive bacteria (staphylococcus aureus, bacillus subtilis, and staphylococcus epidermis), four gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, salm ...	2011	21782421
rising incidence of acute prostatitis following prostate biopsy: fluoroquinolone resistance and exposure is a significant risk factor.	objectives: to evaluate the frequency and potential risk factors for infection-related complications after transrectal prostate biopsy and to propose adjustments in current antimicrobial prophylaxis recommendations. methods: during 2008-2010, 107 patients underwent transrectal ultrasound-guided biopsies of the prostate at our institution. charts were reviewed for infection-related complications within 30 days of the procedure. potential risk factors were evaluated, including age, diabetes mellit ...	2011	21782225
late-onset klebsiella oxytoca flap-margin-related corneal ulcer following laser in situ keratomileusis.	a 45-year-old woman developed a spontaneous flap-margin-related corneal ulcer 11 years after the original laser in situ keratomileusis (lasik) procedure. two enhancements had been performed; the most recent was 3 years prior to presentation. cultures were positive for a heavy growth of klebsiella oxytoca. the ulcer responded clinically to topical treatment with fortified cefazolin. eighteen days after presentation, the infiltrate had resolved and the cornea had fully epithelialized. to our knowl ...	2011	21782101
[clinical and economic expedience of ertapenem therapy of complicated urinary tract infection].	clinical and economic investigation of various antibiotics use in the treatment of complicated urinary tract infection (cuti) was performed under the russian economic environment. the drugs of comparison were ertapenem, ceftriaxone and levofloxacin. direct costs and their structure were shown, and the cost efficiency was calculated. alternative analysis and one-side susceptibility analysis were performed. in complicated urinary tract infections when the major pathogens were escherichia coli, kle ...	2011	21780670
[antimicrobial activity of bacteriocin s760 produced by enterococcus faecium strain lwp760].	antimicrobial activity of bacteriocin s760 (enterocin) produced by enterococcusfaecium strain lwp760 was studied. bacteriocin s760 is a cationic, hydrophobic, and heat stable peptide with the molecular weight of 5.5 kda and pl of 9.8. enterocin s760 is shown to inhibit in vitro the growth both of sensitive and resistant to antibacterials gramnegative and grampositive bacteria of 25 species. mics of the bacteriocin s760 vary between 0.05-1.6 mg/l for escherichia coli 0157:h117, salmonella typhimu ...	2011	21780664
impact of hfq on global gene expression and virulence in klebsiella pneumoniae.	klebsiella pneumoniae is responsible for a wide range of clinical symptoms. how this bacterium adapts itself to ever-changing host milieu is still a mystery. recently, small non-coding rnas (srnas) have received considerable attention for their functions in fine-tuning gene expression at a post-transcriptional level to promote bacterial adaptation. here we demonstrate that hfq, an rna-binding protein, which facilitates interactions between srnas and their mrna targets, is critical for k. pneumon ...	2011	21779404
[a case of emphysematous gastritis in a patient with end-stage renal disease].	emphysematous gastritis is a rare infection of the stomach wall with high mortality rate. it is caused by gas forming organisms and may arise by local spread through the mucosa or hematogenous dissemination from distant focus. clinical manifestation includes acute abdomen with systemic toxicity, and diagnosis is based on radiologic demonstration of gas within the gastric wall. treatment should be aimed to cover gram-negative organisms and anaerobes using wide-spectrum intravenous antibiotics, an ...	2011	21778802
high-mobility group protein n2 (hmgn2) inhibited the internalization of klebsiella pneumoniae into cultured bladder epithelial cells.	since bacterial invasion into host cells is an important step in the infection process, using the agents to interfere with bacterial internalization is an attractive approach to block the infection process. in this work, we describe a new, previously unrecognized role of the human cationic host defense peptide hmgn2 during klebsiella pneumoniae infections. our results revealed that the internalization of k. pneumoniae strain 03183 into cultured bladder epithelial cells (t24) was significantly re ...	2011	21778192
clinical predictors of pseudomonas aeruginosa bacteremia among gram-negative bacterial infections in non-neutropenic patients with solid tumor.	objectives: this study was performed to identify risk factors in pseudomonas aeruginosa bacteremia among gram-negative bacterial infections in non-neutropenic patients with solid tumor. methods: a case-control study was performed to identify clinical predictors for p. aeruginosa bacteremia among non-neutropenic patients with gram-negative bacteremia. each case of p. aeruginosa bacteremia was matched to one or two controls with escherichia coli, klebsiella, enterobacter or citrobacter species in ...	2011	21777616
cryptogenic pyogenic liver abscess as the herald of colon cancer.	background and aim: colonic mucosal defects might be a route for bacterial invasion into the portal system with subsequent hematogenous spread to the liver. we retrospectively investigated the results of colonoscopy and clinical characteristics of patients with pyogenic liver abscess of colonic origin. methods: a total of 230 consecutive patients with pyogenic liver abscess were reviewed between 2003 and 2010. the 230 patients were categorized into three groups [pancreato-biliary (n = 135), cryp ...	2011	21777280
longitudinal survey of carbapenem resistance and resistance mechanisms in enterobacteriaceae and non-fermenters from the usa in 2007-09.	background antibiotic resistance is problematic in enterobacteriaceae, pseudomonas aeruginosa and acinetobacter baumannii, and is often associated with serious infections. carbapenems are often one of the few remaining therapeutic options, so it is important to monitor carbapenem activity against these pathogens and to identify resistance mechanisms. methods carbapenem susceptibilities were determined for 14?359 enterobacteriaceae, 3614 p. aeruginosa and 994 a. baumannii from the usa (2007-09). ...	2011	21775338
crystal structure of new delhi metallo-ß-lactamase reveals molecular basis for antibiotic resistance.	ß-lactams are the most commonly prescribed class of antibiotics and have had an enormous impact on human health. thus, it is disquieting that an enzyme called new delhi metallo-ß-lactamase-1 (ndm-1) can confer enterobacteriaceae with nearly complete resistance to all ß-lactam antibiotics including the carbapenams. we have determined the crystal structure of klebsiella pneumoniae apo-ndm-1 to 2.1-å resolution. from the structure, we see that ndm-1 has an expansive active site with a unique electr ...	2011	21774017
methanolic extracts of aloe secundiflora engl. inhibits in vitro growth of tuberculosis and diarrhea-causing bacteria.	the emergence of resistance to antimicrobials by pathogens has reached crisis levels, calling for identification of alternative means to combat diseases.	2011	21772752
preliminary studies for a new antibiotic from the marine mollusk melo melo (lightfoot, 1786).	to search for new bioactive compounds from marine mollusks melo melo (m. melo).	2011	21771476
bla(ctx-m), bla(tem), and bla(shv) in enterobacteriaceae from north-indian tertiary hospital: high occurrence of combination genes.	to delineate the frequency of occurrence of bla(ctx-m), bla(tem), and bla(shv) in enterobacteriaceae from north-indian tertiary hospital.	2011	21771430
isolation and identification of bioactive antibacterial components in leaf extracts of vangueria spinosa (rubiaceae).	the column chromatographic fraction of ethyl acetate (ea1, ea2, ea3, ea4 and ea5) leaf extracts of vangueria spinosa (v. spinosa) were screened for antibacterial activity and phytochemical analysis.	2011	21771412
transmission of extended-spectrum ß-lactamase klebsiella oxytoca via the breathing circuit of a transport ventilator: root cause analysis and infection control recommendations.		2011	21768772
production of kpc-2 carbapenemase by an escherichia coli clinical isolate belonging to the international st131 clone.	the rapid international dissemination of klebsiella pneumoniae carbapenemase (kpc) producing organisms is of major concern.…	2011	21768521
emergence of oxa-48-producing escherichia coli clone st38 in france.	the bla(oxa-48) gene encodes a carbapenem-hydrolyzing class d ß-lactamase that was first identified in klebsiella pneumoniae from turkey in 2003 and then extensively in that country (1, 11).…	2011	21768512
characterization of oxa-181, a carbapenem-hydrolyzing class d {beta}-lactamase from klebsiella pneumoniae.	klebsiella pneumoniae kp3 was isolated from a patient, transferred from india to the sultanate of oman. k. pneumoniae kp3 was resistant to all ß-lactams including carbapenems and expressed the carbapenem-hydrolyzing ß-lactamase oxa-181, which differs from oxa-48 by four amino acid substitutions. compared to oxa-48, oxa-181 possessed a very similar hydrolytic profile. the bla(oxa-181) gene was located on a 7.6-kb cole-type plasmid and was linked to insertion sequence isecp1. the isecp1-mediated o ...	2011	21768505
laboratory evaluation of the chromagar kpc medium for identification of carbapenem-nonsusceptible enterobacteriaceae.	chromagar kpc was evaluated with 53 carbapenem-nonsusceptible strains. kpc-producing klebsiella pneumoniae and enterobacter cloacae grew efficiently and yielded metallic blue colonies (100% sensitivity). poor or no growth or atypical colony color were evident with kpc-producing escherichia coli (71.4% sensitivity) and ertapenem-resistant kpc-nonproducing k. pneumoniae (72.7% sensitivity). chromagar kpc appears applicable for processing carbapenem-resistant enterobacteriaceae surveillance specime ...	2011	21767716
detection of synergy using the combination of polymyxin b with either meropenem or rifampin against carbapenemase-producing klebsiella pneumoniae.	polymyxin b (pb) plus meropenem (mer) or rifampin (rif) was tested by etest® method and time-kill assay (tka) against 14 genetically unique clinical klebsiella pneumoniae carbapenemase-producing k. pneumoniae. pb + mer: etest, 43% synergy; tka, 64% synergy. concordance between methods was 79%. for pb + rif: etest, 21% synergy; tka, 100% synergy. concordance between methods was 21%.	2011	21767715
accelerated production of 1,3-propanediol from glycerol by klebsiella pneumoniae using the method of forced ph fluctuations.	1,3-propanediol (1,3-pd) is a bivalent alcohol, used in a number of chemical syntheses. it could be produced from glycerol in course of microbial fermentation by klebsiella pneumoniae along with more than five minor liquid products. with the purpose to enhance 1,3-pd production and to eliminate by-products formation, principally new ph control on the process was applied. the method, named "forced ph fluctuations" was realized by consecutive raisings of ph with definite ?ph amplitude (ranging fro ...	2011	21766234
functions of some capsular polysaccharide biosynthetic genes in klebsiella pneumoniae ntuh k-2044.	the growing number of klebsiella pneumoniae infections, commonly acquired in hospitals, has drawn great concern. it has been shown that the k1 and k2 capsular serotypes are the most detrimental strains, particularly to those with diabetes. the k1 cps (capsular polysaccharide) locus in the ntuh-2044 strain of the pyogenic liver abscess (pla) k. pneumoniae has been identified recently, but little is known about the functions of the genes therein. here we report characterization of a group of cps g ...	2011	21765903
long-term clinical and economic benefits associated with the management of a nosocomial outbreak resulting from extended-spectrum beta-lactamase-producing klebsiella pneumoniae.	objective:: in 2005, there was an epidemic of infections resulting from extended-spectrum ß-lactamase-producing klebsiella pneumoniae in the intensive care department. the aim of this study was to evaluate the potential long-term clinical and economic benefits resulting from the management of this epidemic and the resulting changes in practices. design:: two periods were defined: the period leading up to and including the epidemic (2003-2005; period i) and the postepidemic period (2006-2008; per ...	2011	21765349
fate of nitrogen-fixing bacteria in crude oil contaminated wetland ultisol.	the effect of crude oil on the growth of legumes (calopogonium muconoides and centrosema pubescens) and fate of nitrogen-fixing bacteria in wetland ultisol was investigated using standard cultural techniques. the results revealed observable effects of oil on soil physico-chemistry, plant growth and nodulation as well as on densities of heterotrophic, hydrocarbonoclastic and nitrogen fixing bacteria. the effects however varied with different levels (0.5%, 1%, 5%, 10%, 15% and 20%) of pollution. a ...	2011	21755289
iliopsoas abscess and spondylodiscitis by staphylococcus aureus: successful clinical treatment.	although pyogenic abscess of the iliopsoas muscle is considered an uncommon condition, its incidence is on the increase. this abscess may be primary if it occurs without a causal factor; more frequently, it is secondary to gastrointestinal or genitourinary affections, or trauma. the main aetiological agents differ among primary (staphylococcus aureus) and secondary (escherichia coli, klebsiella spp, streptococcus spp, bacteroides spp) abscess. we report the case of a 64-year-old man with iliopso ...	2011	21753252
susceptibility of esbl-producing enterobacteriaceae with the new clsi breakpoints.	in 2010 clsi lowered the susceptibility breakpoints of some cephalosporins and aztreonam for enterobacteriaceae and eliminated the need to perform esbl screening and confirmatory tests. the aim of this study was to determine how many esbl-producing strains will test susceptible for three common species of enterobacteriaceae using the new breakpoints. as determined with the clsi screening and confirmatory tests, 382 consecutive esbl-producing strains were collected at our hospital between 2007 an ...	2011	21752977
antioxidant, electrochemical, thermal, antimicrobial and alkane oxidation properties of tridentate schiff base ligands and their metal complexes.	in this study, two schiff base ligands (hl(1) and hl(2)) and their cu(ii), co(ii), ni(ii), pd(ii) and ru(iii) metal complexes were synthesized and characterized by the analytical and spectroscopic methods. alkane oxidation activities of the metal complexes were studied on cyclohexane as substrate. the ligands and their metal complexes were evaluated for their antimicrobial activity against corynebacterium xerosis, bacillus brevis, bacillus megaterium, bacillus cereus, mycobacterium smegmatis, st ...	2011	21752697
essential oils from moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant gram-negative bacteria.	bacterial drug resistance is a worrying public health problem. antibiotic efflux is a major non-specific resistance mechanism used by bacteria, and efflux pumps are involved in the low-level susceptibility of various important gram-negative pathogens. use of molecules that can block bacterial pumps is an attractive strategy, but several studies report only partial efficacy owing to limits of these molecules (stability, selectivity, bioavailability, toxicity, etc.). the objective of this study wa ...	2011	21752605
impedimetric biosensor based on self-assembled hybrid cystein-gold nanoparticles and cramoll lectin for bacterial lipopolysaccharide recognition.	we report the development of a new selective and specific electrochemical biosensor for bacterial lipolysaccharide (lps). an electrode interface was constructed using a l-cysteine-gold nanoparticle (aunpcys) composite to be immobilized by electrostatic interaction in the network of a poly(vinyl chloride-vinyl acetate maleic acid) (pvm) layer on a gold bare electrode. the impedimetric biosensor is fabricated by self-assembled cramoll lectin on the pvm-aunpcys-modified gold electrode through elect ...	2011	21752390
incidence of esbl producers amongst gram-negative bacilli isolated from intra-abdominal infections across india (based on smart study, 2007 data).	this study was conducted in 9 centers spread over india from january 1 to december 31, 2007 to monitor in vitro susceptibility of gram-negative bacilli to group i carbapenem, ertapenem and other antimicrobials in intra-abdominal infections and to identify early changes in susceptibility pattern of community or hospital acquired organisms, with a focus on esbl producers.	2011	21751604
klebsiella pneumoniae bacteremia and capsular serotypes, taiwan.	capsular serotypes of 225 klebsiella pneumoniae isolates in taiwan were identified by using pcr. patients infected with k1 serotypes (41 isolates) had increased community-onset bacteremia, more nonfatal diseases and liver abscesses, lower pittsburgh bacteremia scores and mortality rates, and fewer urinary tract infections than patients infected with non-k1/k2 serotypes (147 isolates).	2011	21749784
metallo-ß-lactamase-producing enterobacteriaceae isolates at a taiwanese hospital: lack of distinctive phenotypes for screening.	liao i-c, chen h-m, wu j-j, tsai p-f, wang l-r, yan j-j. metallo-ß-lactamase-producing enterobacteriaceae isolates at a taiwanese hospital: lack of distinctive phenotypes for screening. apmis 2011; 119: 543-50. we investigated the prevalence of metallo-ß-lactamases (mbls) among 1,827 enterobacteriaceae isolates collected in 2006 and evaluated the vitek 2 microbiology system, modified hodge test, and 2 combined disk tests as the screening tools for mbls by using these isolates and 77 previously c ...	2011	21749455
first outbreak of a plasmid-mediated carbapenem hydrolyzing oxa-48 {beta}-lactamase in klebsiella pneumoniae in spain.	twenty klebsiella pneumoniae isolates producing oxa-48 were collected from april-2009 to september-2010. strains were clonally related and co-produced a ctx-m-15 ß-lactamase. a conjugative plasmid of circa 70kb carrying bla(oxa-48) was identified. eleven isolates showed low-level resistance to carbapenems, whereas nine showed high-level resistance. decreased expression of ompk36 participated in high-level resistance to carbapenems. this strain belonged to sequence type 101 (st101). this is the f ...	2011	21746954
comparative genomics of klebsiella pneumoniae strains with different antibiotic resistance profiles.	there is a global emergence of multidrug resistant (mdr) strains of klebsiella pneumoniae, a gram-negative enteric bacterium that cause nosocomial and urinary tract infections. while the epidemiology of k. pneumoniae strains and occurrences of specific antibiotic resistance genes, such as plasmid-borne extended spectrum ß-lactamases (esbls), have been extensively studied, only four complete genomes of k. pneumoniae are available. to better understand the mdr factors in k. pneumoniae, we determin ...	2011	21746949
detection of a vim-27-producing klebsiella pneumoniae in a patient following surgical tourism in greece.	very recently papagiannitsis described vim-27, a new metallo-beta-lactamase (mbl) single point variant of vim-1, identified in three klebsiella pneumoniae isolates of sequence type (st) 147 recovered at three different greeks hospitals (6).…	2011	21746946
spread of iscr1 elements containing bladha-1 and multiple antimicrobial resistance genes leading to increase of flomoxef resistance in esbl-producing klebsiella pneumoniae.	increasing resistance to quinolones, aminoglycosides and/or cephamycins in extended-spectrum ß-lactamase (esbl)-producing enterobacteriaceae exacerbates the already limited antibiotic treatment options for infections due to these microbes. in this study, the presence of resistance determinants for these antimicrobial agents was examined by pcr among esbl-producing klebsiella pneumoniae (esbl-kp) isolates that caused bacteremia. pulsed-field gel electrophoresis was used to differentiate the clona ...	2011	21746945
bacterial isolates of early-onset neonatal sepsis and their antibiotic susceptibility pattern between 1998 and 2004: an audit from a center in india.	abstract:	2011	21745376
rapid acquisition of decreased carbapenem susceptibility in a strain of klebsiella pneumoniae arising during meropenem therapy.	clin microbiol infect abstract: a strain of klebsiella pneumoniae (k1) was isolated from a catheterized patient with a urinary tract infection. the patient was subsequently treated with meropenem, after which k. pneumoniae (k2) was once again isolated from the patient's urine. susceptibility testing showed that strain k1 was fully susceptible to carbapenem antibiotics with the exception of ertapenem, to which it exhibited intermediate resistance, whilst k2 was resistant to ertapenem and meropene ...	2011	21745255
molecular analysis of population structure and antibiotic resistance of klebsiella isolates from a three-year surveillance program in florence hospitals, italy.	we report the results of a three-year surveillance program of klebsiella spp. in six hospitals in florence (italy). a total of 172 klebsiella isolates were identified and typed by aflp: 122 were k. pneumoniae and 50 were k. oxytoca. most k. pneumoniae (80%) and k. oxytoca (93%) showed unrelated aflp profiles. beside this heterogeneous population structure, we found five small epidemic clonal groups of k. pneumoniae. four of these groups were involved in outbreak events, three of which occurred i ...	2011	21744038
extended-spectrum beta-lactamase-mediated resistance among bacterial isolates recovered from ocular infections.	purpose: to evaluate resistances mediated by extended-spectrum +¦-lactamases (esbls) and ampc +¦-lactamases among gram-negative bacteria recovered from ocular infections. methods: as per the clinical laboratory standards institute m100-s-16 document, a total of 135 gram-negative bacilli were recovered from ocular specimens and were subjected to phenotypic confirmation for esbl production by the double-disc synergy test, cephalosporin and clavulanate combination disc test and e test, and, for amp ...	2011	21720185
horizontal transfer of antimicrobial resistance by extended-spectrum +¦ lactamase-producing enterobacteriaceae.	the purpose of this work was to study the acquisition of new antibiotic-resistant genes carried by extended spectrum +¦-lactamase (esbl)-producing enterobacteriaceae via horizontal transfer to understand their rampant spread in the hospitals and in the community.	2011	21701662
wide dissemination of extended-spectrum beta-lactamase (esbl)-producing escherichia coli and klebsiella spp. in acute care and rehabilitation hospitals.	summarya prospective surveillance system for extended-spectrum beta-lactamase-producing escherichia coli (esbl-e) and esbl-producing klebsiella spp. (esbl-k) was implemented in 39 german hospitals with the aim of determining the incidence densities (ids) of community-onset and hospital-onset cases and of identifying risk factors for high ids of hospital-onset cases. during 2008, 2081 esbl-e/k cases were documented. esbl-e cases (n=1330, 63-à9%) were more common than esbl-k cases (n=751, 36-à1%), ...	2011	21676355
analysis of the networks controlling the antimicrobial-peptide-dependent induction of klebsiella pneumoniae virulence factors.	antimicrobial peptides (aps) impose a threat to the survival of pathogens, and it is reasonable to postulate that bacteria have developed strategies to counteract them. polymyxins are becoming the last resort to treat infections caused by multidrug-resistant gram-negative bacteria and, similar to aps, they interact with the anionic lipopolysaccharide. given that polymyxins and aps share the initial target, it is possible that bacterial defense mechanisms against polymyxins will be also effective ...	2011	21708987
antimicrobial activity of liposome encapsulated cyclo(l-tyrosyl-l-prolyl).	various studies have shown the potentially beneficial biological activities of cyclic dipeptides and in particular, cyclo(l-tyrosyl-l-prolyl) (cyclo(tyr-pro)) has shown fair antibacterial activity in vitro. this study aimed to determine if liposome encapsulation would have any significant effects on the antibacterial activity of this compound. the thin-film hydration method with extrusion was used to produce small unilamellar vesicles containing cyclo(tyr-pro) that were shown to have an average ...	2011	21699080
klebsiella pneumoniae yggg gene product: a zinc-dependent metalloprotease.	klebsiella pneumoniae causes neonatal sepsis and nosocomial infections. one of the strains, k. pneumoniae mgh 78578, shows high level of resistance to multiple microbial agents. in this study, domain family, amino acid sequence and topology analyses were performed on one of its hypothetical protein, yggg (kpn_03358). structural bioinformatics approaches were used to predict the structure and functionality of yggg protein. the open reading frame (orf) of yggg, which was a putative metalloprotease ...	2011	21845088
prevalence and characterization of plasmid-mediated quinolone resistance genes in extended-spectrum +¦-lactamase-producing enterobacteriaceae isolates in mexico.	the objectives of this study were to investigate the prevalence of plasmid-mediated quinolone resistance genes in a collection of 226 extended-spectrum +¦-lactamase (esbl)-producing enterobacteriaceae isolates and characterize the qnr-positive isolates. the rate of qnr-positive isolates was 21.6% (49/226), 49.5% for aac(6')-ib-cr (112/226), and 1.7% for qepa1 (4/226). those isolates carried qnr genes corresponding to types qnrb (71.4%), qnrs1 (24.4%), and qnra1 (18.3%). the distribution among ba ...	2011	21834663
carbapenem-resistant klebsiella pneumoniae in post-acute-care facilities in israel.	objective.ôçâto assess the prevalence of and risk factors for carbapenem-resistant klebsiella pneumoniae (crkp) carriage among patients in post-acute-care facilities (pacfs) in israel. design, setting, and patients.ôçâa cross-sectional prevalence survey was conducted in 12 pacfs. rectal swab samples were obtained from 1,144 patients in 33 wards. risk factors for crkp carriage were assessed among the cohort. next, a nested, matched case-control study was conducted to define individual risk factor ...	2011	21828964
chemical composition and antibacterial activity of the essential oil of lantana camara var. moritziana.	the essential oil obtained from the leaves of lantana camara var. moritziana (otto & dietr.) l+¦pez-palacios collected at rubio, t+íchira state, venezuela, was obtained by hydrodistillation in a clevenger trap (0.1% yield). the oil was analyzed by gas chromatography-mass spectrometry (gc/ms) on hp gc-ms system, model 5973, identifying 33 compounds (97.1%) of which the major components were germacrene d (31.0%), followed by beta-caryophyllene (14.8%), a-phellandrene (6.7%), limonene (5.7%) and 1, ...	2011	21834251
[evolution of susceptibility to antibiotics of escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and acinetobacter baumanii, in a university hospital center of beirut between 2005-áand 2009.]	introduction: until recently, multiresistant bacteria were only limited to hospitals. however, they are now responsible for community acquired infections, affecting people who have had no contact with the hospital environment. several mechanisms are associated with these resistances. the production of betalactamases is however the predominant mechanism and especially the production of extended spectrum beta-lactamases or esbl by strains of escherichia coli and klebsiella pneumoniae, which mediat ...	2011	21719212
purple urine.		2011	21738253
assessment of the antimicrobial effect of three plants used for therapy of community-acquired urinary tract infection in rio grande do sul (brazil).	ethnopharmacological relevance: the urinary tract infections (uti) are among the infectious diseases of bacterial origin most commonly diagnosed, and may result in a significant mortality. the microorganisms most frequently involved in community-acquired infections are escherichia coli and klebsiella pneumoniae, both gram-negative bacillus of the enterobacteriaceae family. in southern brazil, it is common the treatment of uti using herbs and teas, a tradition inherited from the ancient indigenou ...	2011	21843617
risk factors associated with short-term post-treatment outcomes of clinical mastitis.	the objectives of this study were to characterize 60-d outcomes after treatment of mild (abnormal milk) and moderate (abnormal milk and abnormal udder) cases of clinical mastitis (cm) occurring in a single quarter of cows on wisconsin farms (n=4) and to determine risk factors associated with those outcomes. duplicate milk samples were collected from the affected quarter of each cow for microbiological analysis at the onset of cm (pre) and 21 d later (post). cows were treated only in the affected ...	2011	21700025
acquired antibiotic resistance: are we born with it?	the rapid emergence of antibiotic resistance (ar) is a major public health concern. recent findings on the prevalence of foodborne antibiotic resistant (art) commensal bacteria in ready-to-consume food products suggested that daily food consumption likely serves as a major avenue for disseminating art bacteria from the food chain to the human hosts. to properly assess the impact of various factors including the food chain on ar development in hosts, it is important to determine the baseline of a ...	2011	21821748
fecal colonization of enterobacteriaceae carrying plasmid-mediated quinolone resistance determinants in korea.	the aims of the current study were to investigate the prevalence and molecular characteristics of plasmid-mediated quinolone resistance (pmqr) genes from colonizing fecal organisms and to compare the incidence and subtype of these genes according to bacterial species and hospital at five tertiary-care hospitals in korea. a total of 500 nonduplicated clinical isolates of enterobacteriaceae were obtained from fecal specimens at five tertiary-care hospitals between march and may 2008. the pmqr gene ...	2011	21830908
aetiology of community-acquired urinary tract infection and antimicrobial susceptibility patterns of uropathogens isolated.	antibiotic resistance among uropathogens causing urinary tract infection (uti) is increasing worldwide. in most cases of uti, family physicians can provide empirical treatment without the benefit of a pre-therapy urine culture. knowledge of the aetiology and antimicrobial susceptibility patterns of uropathogens is important in order to determine the best empiric treatment option. this study aimed to determine the aetiology and antimicrobial susceptibility of uropathogens in culture-positive, com ...	2011	21731993
antibacterial activity and cytocompatibility of chitosan-n-hydroxy-2,3-propyl-n methyl-n,n-diallylammonium methyl sulfate.	a water-soluble quaternary ammonium salt of chitosan, chitosan-n-hydroxy-2,3-propyl-n-methyl-n,n-diallylammonium methyl sulfate (mdaacs), was synthesized by reacting chitosan with methyl diallyl ammonium salt (mdaa). the results of water contact angle and swelling ratio showed that the membrane of mdaacs was more hydrophilic than chitosan. the antibacterial activities of mdaacs against staphylococcus aureus and klebsiella pneumoniae were evaluated with the minimum inhibitory concentration (mic) ...	2011	21816584
pharmacodynamics of meropenem in critically ill patients with febrile neutropenia and bacteraemia.	the bactericidal activity of β-lactams is determined by the time that concentrations in tissue and serum are above the minimum inhibitory concentration (t>mic) for the pathogen. the aim of this study was to compare the probability of target attainment (pta) and the cumulative fraction of response (cfr) for meropenem between administration by bolus injection and a 3-h infusion. the study was a randomised, three-way, cross-over design in eight febrile neutropenic patients with bacteraemia. each su ...	2011	21726984
contemporary activity of colistin and polymyxin b against a worldwide collection of gram-negative pathogens: results from the sentry antimicrobial surveillance program (2006-09).	objectives to comparatively evaluate the antimicrobial activities of colistin and polymyxin b with those of other antimicrobials against a worldwide collection of 40ôçè625 gram-negative bacilli. methods antimicrobial susceptibility testing was performed and interpreted using the clsi broth microdilution method except for colistin against enterobacteriaceae. results the polymyxins showed potent in vitro activities (mic(90), ôëñ0.5-1 mg/l) against this large collection of clinical isolates, with v ...	2011	21715434
antimicrobial polyethyleneimine-silver nanoparticles in a stable colloidal dispersion.	excellent colloidal stability and antimicrobial activity are important parameters for silver nanoparticles (agnps) in a range of biomedical applications. in this study, polyethyleneimine (pei)-capped silver nanoparticles (pei-agnps) were synthesized in the presence of sodium borohydride (nabh(4)) and pei at room temperature. the pei-agnps had a positive zeta potential of approximately +49mv, and formed a stable nanocolloid against agglomeration due to electrostatic repulsion. the particle size a ...	2011	21821399
high mobility group nucleosomal binding domain 2 protein protects bladder epithelial cells from klebsiella pneumoniae invasion.	due to the predominance of multiple-antibiotic-resistant klebsiella pneumoniae strains, the search for new approaches for the prevention of k. pneumoniae infections is now under intensive investigation. the objective of the present study was to investigate the effects of high mobility group nucleosomal binding domain 2 (hmgn2) protein, which acts on the bladder epithelial cells t 24, on the invasion of k. pneumoniae 03183 and explore its possible mechanisms. pretreatment with hmgn2 significantly ...	2011	21720014
molecular and epidemiological analysis of nosocomial carbapenem-resistant klebsiella spp. using repetitive extragenic palindromic-polymerase chain reaction and matrix-assisted laser desorption/ionization-time of flight.	introduction: infections with carbapenem-resistant enterobacteria are an emerging threat. this study reports the microbiologic, clinical, and epidemiologic features and the therapeutic outcomes of the infections caused by carbapenem- and pandrug-resistant klebsiella emerged in our hospital. fingerprinting analyses by automated repetitive extragenic palindromic-polymerase chain reaction (rep-pcr) and matrix-assisted laser desorption/ionization-time of flight (maldi-tof) mass spectrometry are also ...	2011	21568752
changing pattern of antibiotic susceptibility in intensive care units: ten years experience of a university hospital.	the study was performed to assess microorganisms and antibiotic susceptibility patterns during ten years in intensive care units of a university hospital. infection control committee has active, prospective surveillance in icus for thirteen years. ten years data of icus was evaluated retrospectively from surveillance forms. microorganisms and their antibiotic resistance were recorded according to the years. during ten years, gram negative microorganisms were the most frequent isolated microorgan ...	2011	21569859
evaluation of sulfatase-directed quinone methide traps for proteomics.	sulfatases hydrolytically cleave sulfate esters through a unique catalytic aldehyde, which is introduced by a posttranslational oxidation. to profile active sulfatases in health and disease, activity-based proteomic tools are needed. herein, quinone methide (qm) traps directed against sulfatases are evaluated as activity-based proteomic probes (abpps). starting from a p-fluoromethylphenyl sulfate scaffold, enzymatically generated qm-traps can inactivate bacterial aryl sulfatases from pseudomonas ...	2011	21570853
population dynamics of bacteria associated with different strains of the pine wood nematode bursaphelenchus xylophilus after inoculation in maritime pine (pinus pinaster).	for a long time it was thought that bursaphelenchus xylophilus was the only agent of the pine wilt disease. recently, it was discovered that there are bacteria associated with the nematodes that contribute to the pathogenesis of this disease, mainly through the release of toxins that promote the death of the pines. among the species most commonly found, are bacteria belonging to the bacillus, pantoea, pseudomonas and xanthomonas genera. the main objective of this work was to study the effect of ...	2011	21570967
type 3 fimbriae and biofilm formation are regulated by the transcriptional regulators mrkhi in klebsiella pneumoniae.	klebsiella pneumoniae is an opportunistic pathogen which frequently causes hospital-acquired urinary and respiratory tract infections. k. pneumoniae may establish these infections in vivo following adherence, using the type 3 fimbriae, to indwelling devices coated with extracellular matrix components. using a colony immunoblot screen, we identified transposon insertion mutants which were deficient for type 3 fimbrial surface production. one of these mutants possessed a transposon insertion withi ...	2011	21571997
[the outcome of monitoring health care-associated bloodstream infections in pardubice regional hospital].	the aim of the study were to find out the incidence of health care-associated bloodstream infections in the pardubice regional hospital (prh) and to introduce a system of the regular monitoring of these infections.	2011	21574130
role of shv ß-lactamase variants in resistance of clinical klebsiella pneumoniae strains to ß-lactams in an algerian hospital.	three clinical klebsiella pneumoniae strains, kparg74, kparg220 and kparg185, isolated from a hospital in algeria, carried the novel ß-lactamases shv-98, shv-99 and shv-100, respectively, and co-expressed tem-1 and either ctx-m-3 or ctx-m-15. in contrast, transformed cells possessing the genes for these novel ß-lactamases, i.e. ecdh5a-shv-98, ecdh5a-shv-99 and ecdh5a-shv-100, respectively, carried unique sequence features of bla(shv) gene variants, enabling oxyimino-cephalosporin susceptibility ...	2011	21415202
preliminary investigation of a mice model of klebsiella pneumoniae subsp. ozaenae induced pneumonia.	in the present study, we comparatively assessed the pathophysiological mechanisms developed during lung infection of balb/c female mice infected by an original wild type klebsiella pneumoniae subsp. ozaenae strain (ch137) or by a referent subspecies k. pneumoniae. subsp. pneumoniae strain (atcc10031). the mice infected with 2.10(6)-ácfu k. p. subsp. pneumoniae (n-á=-á10) showed transient signs of infection and all of them recovered. all of those infected with 1.10(6)-ácfu k. p. subsp. ozaenae (n ...	2011	21723409
development of autoimmune diabetes in the absence of detectable il-17a in a cd8-driven virally induced model.	recent studies have shown that il-17 can contribute beneficially to pathogen defense but also that excessive il-17 levels are associated with chronic inflammation and autoimmune disorders. to date, the role of il-17 in viral infections and type 1 diabetes is ambiguous. in this study, we used il-17a enhanced green fluorescent protein bicistronic reporter mouse strains to analyze in situ production of il-17a. upon klebsiella pneumoniae bacterial infection, cd4(+) and +¦+¦ t cells produce il-17a. i ...	2011	21832162
[comparison of two phenotypic methods for detecting the production of extended-spectrum beta-lactamases by hospital klebsiella pneumoniae strains].	in the period 2006-2008, the republican emergence medical care center.isolated 2997 enterobacteria, 48.9% of which belonged to klebsiella pneumoniae. confirming phenotypic methods, such as double disk method (ddm) and double disk synergy test (ddst), were used to examine 64 k. pneumoniae strains that had been regarded as being suspected to produce extended-spectrum beta-lactamases (esbl) during a preliminary screening. of the 64 k. pneumoniae strains, 6 (9.4%) were unsusceptible to clavulanate a ...	2011	21735683
fluoroquinolone resistance in escherichia coli and klebsiella pneumoniae over 18 years: effect of different systems for eliminating duplicates.		2011	21693462
first report of a novel extended-spectrum beta-lactamase koxy-2 producing klebsiella oxytoca that hydrolyses cefotaxime and ceftazidime.	klebsiella oxytoca strains mu946294n and mb193997e were isolated from patients in scotland. strain mu946294n was resistant to pencillins, monbactams and cephalosporins. isolate mb193997e displayed a +¦-lactam resistance phenotype consistent with chromosomal +¦-lactamase overproduction. no bla(tem), bla(shv) or bla(ctx-m) genes could be amplified in either strain; however, amplification by pcr was found with primers for the bla(oxy-2) gene. this +¦-lactamase gene in mu946294n differed by one muta ...	2011	21742579
cloning, expression, and enzymatic activity of acinetobacter baumannii and klebsiella pneumoniae acetyl-coenzyme a carboxylases.	pathogenic gram-negative bacteria are a major public health concern because they are causative agents of life-threatening hospital-acquired infections. due to the increasing rates of resistance to available antibiotics, there is an urgent need to develop new drugs. acetyl-coenzyme a carboxylase (accase) is a promising target for the development of novel antibiotics. we describe here the expression, purification, and enzymatic activity of recombinant accases from two clinically relevant gram-nega ...	2011	21704013
genotypic characterization and in vitro activities of tigecycline and polymyxin b for enterobacteriaceae with decreased susceptibility to carbapenems.	carbapenam-resistant enterobacteriaceae is increasing. in order to evaluate the effects of tigecycline and polymyxin b against the carbapenam-non-susceptible pathogens, 89 representative clinical isolates of carbapenem-non-susceptible enterobacteriaceae were recovered from 7 hospitals of 4 cities in china during 2006 to 2009, including 30 serratia marcescens, 35 klebsiella pneumoniae, 7 enterobacter cloacae, 6 enterobacter aerogenes, 5 escherichia coli, 4 citrobacter freundii, and 2 klebsiella o ...	2011	21835972
in vitro spectrum of activity of finafloxacin, a novel, ph-activated fluoroquinolone, under standard and acidic conditions.	finafloxacin is a novel fluoroquinolone that exhibits enhanced antibacterial activity under acidic conditions. the aim of this study was to define the in vitro ph-activity relationship. finafloxacin exhibited optimal antibacterial activity between ph 5.0 and 6.0 at which mics were 4- to 8-fold lower than those determined at neutral ph. these observations were then confirmed against a larger collection of bacteria. these data suggest that finafloxacin could potentially offer a therapeutic advanta ...	2011	21709094