Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time pcr assay and melt curve analysis. | metallo-beta-lactamase enzymes (mbetal) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. the objective of this study was to develop a multiplex real-time pcr assay followed by a melt curve step for rapid detection and identification of genes encoding mbetal-type enzymes based on the amplicon melting peak. the reference sequences of all genes encoding imp and vim types, spm-1, gim-1, and sim-1 were downloaded from genbank, and primers were designed t ... | 2007 | 17093019 |
| benzoate catabolite repression of the phthalate degradation pathway in rhodococcus sp. strain dk17. | rhodococcus sp. strain dk17 exhibits a catabolite repression-like response when provided simultaneously with benzoate and phthalate as carbon and energy sources. benzoate in the medium is depleted to detection limits before the utilization of phthalate begins. the transcription of the genes encoding benzoate and phthalate dioxygenase paralleled the substrate utilization profile. two mutant strains with defective benzoate dioxygenases were unable to utilize phthalate in the presence of benzoate, ... | 2007 | 17158614 |
| benzoate catabolite repression of the phthalate degradation pathway in rhodococcus sp. strain dk17. | rhodococcus sp. strain dk17 exhibits a catabolite repression-like response when provided simultaneously with benzoate and phthalate as carbon and energy sources. benzoate in the medium is depleted to detection limits before the utilization of phthalate begins. the transcription of the genes encoding benzoate and phthalate dioxygenase paralleled the substrate utilization profile. two mutant strains with defective benzoate dioxygenases were unable to utilize phthalate in the presence of benzoate, ... | 2007 | 17158614 |
| distinct antimicrobial resistance patterns and antimicrobial resistance-harboring genes according to genomic species of acinetobacter isolates. | using 58 isolates of acinetobacter species recovered from a university hospital between august 2004 and march 2005, we performed genomic identification by amplified rrna gene restriction analysis (ardra) and investigated the existence of metallo-beta-lactamase (mbl) producers and extended-spectrum beta-lactamase (esbl) producers. genomic species identification of acinetobacter strains using ardra showed that 40 strains were genomic species 2 (acinetobacter baumannii), 9 were 13 sensu tjernberg a ... | 2007 | 17192417 |
| distinct antimicrobial resistance patterns and antimicrobial resistance-harboring genes according to genomic species of acinetobacter isolates. | using 58 isolates of acinetobacter species recovered from a university hospital between august 2004 and march 2005, we performed genomic identification by amplified rrna gene restriction analysis (ardra) and investigated the existence of metallo-beta-lactamase (mbl) producers and extended-spectrum beta-lactamase (esbl) producers. genomic species identification of acinetobacter strains using ardra showed that 40 strains were genomic species 2 (acinetobacter baumannii), 9 were 13 sensu tjernberg a ... | 2007 | 17192417 |
| in silico panning for a non-competitive peptide inhibitor. | peptide ligands have tremendous therapeutic potential as efficacious drugs. currently, more than 40 peptides are available in the market for a drug. however, since costly and time-consuming synthesis procedures represent a problem for high-throughput screening, novel procedures to reduce the time and labor involved in screening peptide ligands are required. we propose the novel approach of 'in silico panning' which consists of a two-stage screening, involving affinity selection by docking simula ... | 2007 | 17222344 |
| innate-immunity cytokines induced by very small size proteoliposomes, a neisseria-derived immunological adjuvant. | neisserial outer membrane proteins have been combined with monosialoganglioside gm3 to form very small size proteoliposomes (vssp), a nanoparticulated formulation used as a cancer vaccine for the treatment of cancer patients with gm3-positive tumours. vssp were shown to elicit anti-gm3 and anti-tumour immune responses. vssp have also been shown to be an efficient adjuvant for tumour-cell and peptide-antigen vaccines in mice. in vitro studies showed that vssp promote maturation of both murine and ... | 2007 | 17223981 |
| antimicrobial susceptibility among acinetobacter calcoaceticus-baumannii complex and enterobacteriaceae collected as part of the tigecycline evaluation and surveillance trial. | to measure the in vitro activity of a panel of antimicrobial agents against gram-negative pathogens collected from the nine census regions of the usa. | 2007 | 17250897 |
| lipolytic enzymes in myxococcus xanthus. | the genome of myxococcus xanthus encodes lipolytic enzymes in three different families: patatin lipases, alpha/beta hydrolases, and gdsl lipases. one member of each family was characterized. the protein encoded by mxan_3852 contains motifs characteristic of patatins. mxan_5522 encodes a protein with the g-x-s-x-g motif characteristic of the lipase subfamily of alpha/beta hydrolases. mxan_4569 encodes a member of the gdsl family of lipolytic enzymes. strains with deletions of mxan_5522 and mxan_4 ... | 2007 | 17307851 |
| gene targeting in gram-negative bacteria by use of a mobile group ii intron ("targetron") expressed from a broad-host-range vector. | mobile group ii introns ("targetrons") can be programmed for insertion into virtually any desired dna target with high frequency and specificity. here, we show that targetrons expressed via an m-toluic acid-inducible promoter from a broad-host-range vector containing an rk2 minireplicon can be used for efficient gene targeting in a variety of gram-negative bacteria, including escherichia coli, pseudomonas aeruginosa, and agrobacterium tumefaciens. targetrons expressed from donor plasmids introdu ... | 2007 | 17322321 |
| genetic characterization of 2,4,6-trichlorophenol degradation in cupriavidus necator jmp134. | the degradation pathway of 2,4,6-trichlorophenol (2,4,6-tcp), a hazardous pollutant, in the aerobic bacterium cupriavidus necator jmp134(pjp4) (formerly ralstonia eutropha jmp134) is encoded by the tcp genes. these genes are located in a genetic context, tcprxabcyd, which resembles a putative catabolic operon. in this work, these gene sequences were individually disrupted and mutant strains were evaluated for their ability to grow on or degrade 2,4,6-tcp. the tcpx and tcpa mutants completely fai ... | 2007 | 17322325 |
| differential response of etiolated pea seedlings to inoculation with rhizobacteria capable of utilizing 1-aminocyclopropane-1-carboxylate or l-methionine. | the majority of soil microorganisms can derive ethylene from l-methionine (l-met), while some rhizobacteria can hydrolyze 1-aminocyclopropane-1-carboxylate (acc) due to their acc-deaminase activity. in this study, three strains having either acc-deaminase activity (pseudomonas putida biotype a, a7), or the ability to produce ethylene from l-met (acinetobacter calcoaceticus, m9) or both (pseudomonas fluorescens, am3) were used for inoculation. the highly ethylene specific bioassay of a classical ... | 2007 | 17342050 |
| adeabc multidrug efflux pump is associated with decreased susceptibility to tigecycline in acinetobacter calcoaceticus-acinetobacter baumannii complex. | to investigate the role of the adeabc multidrug efflux pump in the decreased susceptibility of clinical isolates of acinetobacter calcoaceticus-acinetobacter baumannii complex to tigecycline. | 2007 | 17363424 |
| a detailed analysis of 16s ribosomal rna gene segments for the diagnosis of pathogenic bacteria. | bacterial 16s ribosomal rna (rrna) genes contain nine "hypervariable regions" (v1-v9) that demonstrate considerable sequence diversity among different bacteria. species-specific sequences within a given hypervariable region constitute useful targets for diagnostic assays and other scientific investigations. no single region can differentiate among all bacteria; therefore, systematic studies that compare the relative advantage of each region for specific diagnostic goals are needed. we characteri ... | 2007 | 17391789 |
| a marine mesorhizobium sp. produces structurally novel long-chain n-acyl-l-homoserine lactones. | our study focused on a mesorhizobium sp. that is phylogenetically affiliated by 16s rrna gene sequence to other marine and saline bacteria of this genus. liquid chromatography-mass spectrometry investigations of the extract obtained from solid-phase extraction of cultures of this bacterium indicated the presence of several n-acyl homoserine lactones (ahls), with chain lengths of c(10) to c(16). chromatographic separation of the active bacterial extract yielded extraordinarily large amounts of tw ... | 2007 | 17400774 |
| peptoniphilus gorbachii sp. nov., peptoniphilus olsenii sp. nov., and anaerococcus murdochii sp. nov. isolated from clinical specimens of human origin. | three groups of previously unknown gram-positive, anaerobic, coccus-shaped bacteria were characterized using phenotypic and molecular taxonomic methods. phenotypic and genotypic data demonstrate that these organisms are distinct, and each group represents a previously unknown subline within clostridium cluster xiii. two groups are most closely related to peptoniphilus harei in the genus peptoniphilus, and the other group is most closely related to anaerococcus lactolyticus in the genus anaerococ ... | 2007 | 17428937 |
| potential of the adhesion of bacteria isolated from drinking water to materials. | heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rdna gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity (contact angle measurements) and ii. by measuring adherence to eight different substrata (asi 304 and 316 stainless steel, copper, polyvinyl chloride, polypropylene, polyethylene, ... | 2007 | 17440920 |
| pantoea agglomerans, a plant pathogen causing human disease. | we present 53 pediatric cases of pantoea agglomerans infections cultured from normally sterile sites in patients seen at a children's hospital over 6 years. isolates included 23 from the bloodstream, 14 from abscesses, 10 from joints/bones, 4 from the urinary tract, and 1 each from the peritoneum and the thorax. p. agglomerans was most associated with penetrating trauma by vegetative material and catheter-related bacteremia. | 2007 | 17442803 |
| a polyomic approach to elucidate the fluoranthene-degradative pathway in mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 is capable of degrading a wide range of high-molecular-weight polycyclic aromatic hydrocarbons (pahs), including fluoranthene. we used a combination of metabolomic, genomic, and proteomic technologies to investigate fluoranthene degradation in this strain. thirty-seven fluoranthene metabolites including potential isomers were isolated from the culture medium and analyzed by high-performance liquid chromatography, gas chromatography-mass spectrometry, and uv-visibl ... | 2007 | 17449607 |
| protein engineering of wzc to generate new emulsan analogs. | acinetobacter venetianus rag1 produces an extracellular, polymeric lipoheteropolysaccharide termed apoemulsan. this polymer is putatively produced via a wzy-dependent pathway. according to this model, the length of the polymer is regulated by polysaccharide-copolymerase (pcp) protein. a highly conserved proline and glycine motif was identified in all members of the pcp family of proteins and is involved in regulation of polymer chain length. in order to control the structure of apoemulsan, defin ... | 2007 | 17449706 |
| lipid composition and transcriptional response of mycobacterium tuberculosis grown under iron-limitation in continuous culture: identification of a novel wax ester. | the low level of available iron in vivo is a major obstacle for microbial pathogens and is a stimulus for the expression of virulence genes. in this study, mycobacterium tuberculosis h37rv was grown aerobically in the presence of limited iron availability in chemostat culture to determine the physiological response of the organism to iron-limitation. a previously unidentified wax ester accumulated under iron-limited growth, and changes in the abundance of triacylglycerol and menaquinone were als ... | 2007 | 17464057 |
| hexadecane and tween 80 stimulate lipase production in burkholderia glumae by different mechanisms. | burkholderia glumae strain pg1 produces a lipase of biotechnological relevance. lipase production by this strain and its derivative lu8093, which was obtained through classical strain improvement, was investigated under different conditions. when 10% hexadecane was included in the growth medium, lipolytic activity in both strains could be increased approximately 7-fold after 24 h of growth. hexadecane also stimulated lipase production in a strain containing the lipase gene fused to the tac promo ... | 2007 | 17468265 |
| comparison of one-tube multiplex pcr, automated ribotyping and intergenic spacer (its) sequencing for rapid identification of acinetobacter baumannii. | acinetobacter baumannii has emerged as a serious cause of nosocomial infections. rapid identification of this pathogen is required so that appropriate therapy can be given and outbreaks controlled. this study evaluated a multiplex pcr and an automated ribotyping system for the rapid identification of acinetobacter baumannii. in total, 22 different reference strains and 138 clinical isolates of acinetobacter spp., identified by 16s-23s rrna intergenic spacer (its) sequence analysis, were evaluate ... | 2007 | 17488329 |
| [(3)h]adenine is a suitable radioligand for the labeling of g protein-coupled adenine receptors but shows high affinity to bacterial contaminations in buffer solutions. | [(3)h]adenine has previously been used to label the newly discovered g protein-coupled murine adenine receptors. recent reports have questioned the suitability of [(3)h]adenine for adenine receptor binding studies because of curious results, e.g. high specific binding even in the absence of mammalian protein. in this study, we showed that specific [(3)h]adenine binding to various mammalian membrane preparations increased linearly with protein concentration. furthermore, we found that tris-buffer ... | 2007 | 18404448 |
| acinetobacter pneumonia: a review. | acinetobacter species are becoming a major cause of nosocomial infections, including hospital-acquired and ventilator-associated pneumonia. acinetobacter species have become increasingly resistant to antibiotics over the past several years and currently present a significant challenge in treating these infections. physicians now rely on older agents, such as polymyxins (colistin), for treatment. this paper reviews the epidemiology, treatment, and prevention of this emerging pathogen. | 2007 | 18092011 |
| a new method to extract dental pulp dna: application to universal detection of bacteria. | dental pulp is used for pcr-based detection of dna derived from host and bacteremic microorganims. current protocols require odontology expertise for proper recovery of the dental pulp. dental pulp specimen exposed to laboratory environment yields contaminants detected using universal 16s rdna-based detection of bacteria. | 2007 | 17957246 |
| epidemiological and microbiological analysis of ventilator-associated pneumonia patients in a public teaching hospital. | ventilator-associated pneumonia (vap) is the most commonly-acquired infection in patients in intensive care units. we analyzed epidemiological and microbiological characteristics and the outcome, in a cohort of critically-ill patients with confirmed diagnosis of vap. all patients who had been on mechanical ventilation (mv) for more than 48 hours were included in our study; material collection for microbiological analysis was done within the first 24 hours after beginning treatment or after chang ... | 2007 | 17962875 |
| spectroscopic and electronic structure study of the enzyme-substrate complex of intradiol dioxygenases: substrate activation by a high-spin ferric non-heme iron site. | various mechanisms have been proposed for the initial o(2) attack in intradiol dioxygenases based on different electronic descriptions of the enzyme-substrate (es) complex. we have examined the geometric and electronic structure of the high-spin ferric es complex of protocatechuate 3,4-dioxygenase (3,4-pcd) with uv/visible absorption, circular dichroism (cd), magnetic cd (mcd), and variable-temperature variable-field (vtvh) mcd spectroscopies. the experimental data were coupled with dft and indo ... | 2007 | 17256852 |
| assessment of technological options and economical feasibility for cyanophycin biopolymer and high-value amino acid production. | major transitions can be expected within the next few decades aiming at the reduction of pollution and global warming and at energy saving measures. for these purposes, new sustainable biorefinery concepts will be needed that will replace the traditional mineral oil-based synthesis of specialty and bulk chemicals. an important group of these chemicals are those that comprise n-functionalities. many plant components contained in biomass rest or waste stream fractions contain these n-functionaliti ... | 2007 | 17876577 |
| insertion sequence diversity in archaea. | insertion sequences (iss) can constitute an important component of prokaryotic (bacterial and archaeal) genomes. over 1,500 individual iss are included at present in the isfinder database (www-is.biotoul.fr), and these represent only a small portion of those in the available prokaryotic genome sequences and those that are being discovered in ongoing sequencing projects. in spite of this diversity, the transposition mechanisms of only a few of these ubiquitous mobile genetic elements are known, a ... | 2007 | 17347521 |
| quantitative determination of free-dna uptake in river bacteria at the single-cell level by in situ rolling-circle amplification. | detection of plasmid dna uptake in river bacteria at the single-cell level was carried out by rolling-circle amplification (rca). uptake of a plasmid containing the green fluorescent protein gene (gfp) by indigenous bacteria from two rivers in osaka, japan, was monitored for 506 h using this in situ gene amplification technique with optimized cell permeabilization conditions. plasmid uptake determined by in situ rca was compared to direct counts of cells expressing gfp under fluorescence microsc ... | 2006 | 16957252 |
| empiric broad-spectrum antibiotic therapy of nosocomial pneumonia in the intensive care unit: a prospective observational study. | antibiotic de-escalation, which consists of the initial institution of empiric broad-spectrum antibiotics followed by antibiotic streamlining driven by microbiological documentation, is thought to provide maximum benefit for the individual patient, while reducing the selection pressure for resistance. | 2006 | 16704742 |
| in vitro and in vivo antibacterial activities of dw-224a, a new fluoronaphthyridone. | dw-224a showed the most potent in vitro activity among the quinolone compounds tested against clinical isolates of gram-positive bacteria. against gram-negative bacteria, dw-224a was slightly less active than the other fluoroquinolones. the in vivo activities of dw-224a against gram-positive bacteria were more potent than those of other quinolones. | 2006 | 16723601 |
| tigecycline: an evidence-based review of its antibacterial activity and effectiveness in complicated skin and soft tissue and intraabdominal infections. | there is an urgent need for novel agents to manage serious bacterial infections, particularly those contracted in healthcare facilities. tigecycline is a novel broad-spectrum glycylcycline with good activity against gram-positive, many gram-negative, anaerobic, and some atypical pathogens that has been developed to address this need. | 2006 | 22500153 |
| biocidal efficacy, biofilm-controlling function, and controlled release effect of chloromelamine-based bioresponsive fibrous materials. | in this study, 2-amino-4-chloro-6-hydroxy-s-triazine (acht) was synthesized through controlled hydrolysis of 2-amino-4,6-dichloro-s-triazine (adct). a simple pad-dry-cure approach was employed to immobilize acht onto cellulosic fibrous materials. after treatment with diluted chlorine bleach, the covalently bound acht moieties were transformed into chloromelamines. the structures of the samples were fully characterized with nmr, uv/vis, dsc, tg, iodometric titration and elemental analyses. the ch ... | 2006 | 17184837 |
| biocidal efficacy, biofilm-controlling function, and controlled release effect of chloromelamine-based bioresponsive fibrous materials. | in this study, 2-amino-4-chloro-6-hydroxy-s-triazine (acht) was synthesized through controlled hydrolysis of 2-amino-4,6-dichloro-s-triazine (adct). a simple pad-dry-cure approach was employed to immobilize acht onto cellulosic fibrous materials. after treatment with diluted chlorine bleach, the covalently bound acht moieties were transformed into chloromelamines. the structures of the samples were fully characterized with nmr, uv/vis, dsc, tg, iodometric titration and elemental analyses. the ch ... | 2006 | 17184837 |
| antagonistic interactions between honey bee bacterial symbionts and implications for disease. | honey bees, apis mellifera, face many parasites and pathogens and consequently rely on a diverse set of individual and group-level defenses to prevent disease. one route by which honey bees and other insects might combat disease is through the shielding effects of their microbial symbionts. bees carry a diverse assemblage of bacteria, very few of which appear to be pathogenic. here we explore the inhibitory effects of these resident bacteria against the primary bacterial pathogen of honey bees, ... | 2006 | 16551367 |
| 16s rrna gene sequencing versus the api 20 ne system and the vitek 2 id-gnb card for identification of nonfermenting gram-negative bacteria in the clinical laboratory. | over a period of 26 months, we have evaluated in a prospective fashion the use of 16s rrna gene sequencing as a means of identifying clinically relevant isolates of nonfermenting gram-negative bacilli (non-pseudomonas aeruginosa) in the microbiology laboratory. the study was designed to compare phenotypic with molecular identification. results of molecular analyses were compared with two commercially available identification systems (api 20 ne, vitek 2 fluorescent card; biomérieux, marcy l'etoil ... | 2006 | 16597863 |
| nosocomial bacteremia due to an as yet unclassified acinetobacter genomic species 17-like strain. | we describe a case of bacteremia due to an as yet unclassified acinetobacter genomic species 17-like strain. the recognition of this microorganism as non-acinetobacter baumannii may have important epidemiological implications, as it relieves the hospital of the implementation of barrier precautions for patients infected or colonized as may be necessary with a multiresistant a. baumannii epidemic. | 2006 | 16597902 |
| culturability of stream bacteria assessed at the assemblage and population levels. | lotic bacterial communities can be examined at multiple levels: from the assemblage level to populations of individual species. in stream environments, as in many other systems, the percentage of bacteria that are culturable is quite low. in this study, the culturability of the overall bacterial assemblage, as well as the culturability of three common species (acinetobacter calcoaceticus, burkholderia cepacia, and pseudomonas putida), was determined in samples collected from four streams on thre ... | 2006 | 16598638 |
| rapid detection of brucella spp. in blood cultures by fluorescence in situ hybridization. | brucellosis is a severe systemic disease in humans. we describe a new 16s rrna-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of brucella and that can be applied directly to positive blood cultures. | 2006 | 16672413 |
| proteomic insights into metabolic adaptations in alcanivorax borkumensis induced by alkane utilization. | alcanivorax borkumensis is a ubiquitous marine petroleum oil-degrading bacterium with an unusual physiology specialized for alkane metabolism. this "hydrocarbonoclastic" bacterium degrades an exceptionally broad range of alkane hydrocarbons but few other substrates. the proteomic analysis presented here reveals metabolic features of the hydrocarbonoclastic lifestyle. specifically, hexadecane-grown and pyruvate-grown cells differed in the expression of 97 cytoplasmic and membrane-associated prote ... | 2006 | 16707669 |
| a constitutively expressed, truncated umudc operon regulates the reca-dependent dna damage induction of a gene in acinetobacter baylyi strain adp1. | in response to environmentally caused dna damage, sos genes are up-regulated due to reca-mediated relief of lexa repression. in escherichia coli, the sos umudc operon is required for dna damage checkpoint functions and for replicating damaged dna in the error-prone process called sos mutagenesis. in the model soil bacterium acinetobacter baylyi strain adp1, however, the content, regulation, and function of the umudc operon are unusual. the umuc gene is incomplete, and a remnant of an isehe3-like ... | 2006 | 16751513 |
| energetics and surface properties of pseudomonas putida dot-t1e in a two-phase fermentation system with 1-decanol as second phase. | the solvent-tolerant strain pseudomonas putida dot-t1e was grown in batch fermentations in a 5-liter bioreactor in the presence and absence of 10% (vol/vol) of the organic solvent 1-decanol. the growth behavior and cellular energetics, such as the cellular atp content and the energy charge, as well as the cell surface hydrophobicity and charge, were measured in cells growing in the presence and absence of 1-decanol. although the cells growing in the presence of 1-decanol showed an about 10% redu ... | 2006 | 16751536 |
| crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding. | the soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. an efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. similar cross-seeding protocols to grow crystals of homologues from escherichia coli and streptomyces coelicolor are described. | 2006 | 16754970 |
| design and evaluation of 16s rrna sequence based oligonucleotide probes for the detection and quantification of comamonas testosteroni in mixed microbial communities. | the beta-proteobacterial species comamonas testosteroni is capable of biotransformation and also biodegradation of a range of chemical compounds and thus potentially useful in chemical manufacturing and bioremediation. the ability to detect and quantify members of this species in mixed microbial communities thus may be desirable. | 2006 | 16772028 |
| cytosolic triacylglycerol biosynthetic pathway in oilseeds. molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase. | triacylglycerols (tags) are the most important storage form of energy for eukaryotic cells. tag biosynthetic activity was identified in the cytosolic fraction of developing peanut (arachis hypogaea) cotyledons. this activity was naf insensitive and acyl-coenzyme a (coa) dependent. acyl-coa:diacylglycerol acyltransferase (dgat) catalyzes the final step in tag biosynthesis that acylates diacylglycerol to tag. soluble dgat was identified from immature peanuts and purified by conventional column chr ... | 2006 | 16798944 |
| acinetobacter skin colonization of us army soldiers. | to evaluate whether skin colonization with acinetobacter calcoaceticus-baumannii complex exists in a population of healthy, nondeployed us army soldiers and, if present, how it might relate to the infections seen in current war casualties. | 2006 | 16807838 |
| soluble aldose sugar dehydrogenase from escherichia coli: a highly exposed active site conferring broad substrate specificity. | a water-soluble aldose sugar dehydrogenase (asd) has been purified for the first time from escherichia coli. the enzyme is able to act upon a broad range of aldose sugars, encompassing hexoses, pentoses, disaccharides, and trisaccharides, and is able to oxidize glucose to gluconolactone with subsequent hydrolysis to gluconic acid. the enzyme shows the ability to bind pyrroloquinoline quinone (pqq) in the presence of ca2+ in a manner that is proportional to its catalytic activity. the x-ray struc ... | 2006 | 16864586 |
| how long do nosocomial pathogens persist on inanimate surfaces? a systematic review. | inanimate surfaces have often been described as the source for outbreaks of nosocomial infections. the aim of this review is to summarize data on the persistence of different nosocomial pathogens on inanimate surfaces. | 2006 | 16914034 |
| knockout and overexpression of pyrroloquinoline quinone biosynthetic genes in gluconobacter oxydans 621h. | in gluconobacter oxydans, pyrroloquinoline quinone (pqq) serves as the cofactor for various membrane-bound dehydrogenases that oxidize sugars and alcohols in the periplasm. proteins for the biosynthesis of pqq are encoded by the pqqabcde gene cluster. our reverse transcription-pcr and promoter analysis data indicated that the pqqa promoter represents the only promoter within the pqqabcde cluster of g. oxydans 621h. pqq overproduction in g. oxydans was achieved by transformation with the plasmid- ... | 2006 | 16936032 |
| identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in mycobacterium tuberculosis under stress. | mycobacterium tuberculosis under stress stores triacylglycerol (tg). there are 15 genes in m. tuberculosis that belong to a novel family of tg synthase genes (tgs), but it is not known which of them is responsible for this accumulation of tg. in this paper, it is reported that m. tuberculosis h37rv accumulated tg under acidic, static or hypoxic growth conditions, or upon treatment with no, whereas tg accumulation was drastically reduced in the tgs1 (rv3130c) disrupted mutant. complementation wit ... | 2006 | 16946266 |
| microbial population dynamics associated with crude-oil biodegradation in diverse soils. | soil bacterial population dynamics were examined in several crude-oil-contaminated soils to identify those organisms associated with alkane degradation and to assess patterns in microbial response across disparate soils. seven soil types obtained from six geographically distinct areas of the united states (arizona, oregon, indiana, virginia, oklahoma, and montana) were used in controlled contamination experiments containing 2% (wt/wt) crude oil spiked with [1-(14)c]hexadecane. microbial populati ... | 2006 | 16957258 |
| impact of acinetobacter infection on the mortality of burn patients. | acinetobacter calcoaceticus-baumannii complex (acb) is recognized as an important cause of nosocomial infections. although acb can be associated with multidrug resistance, its impact on mortality in burn patients has not been fully elucidated. | 2006 | 17000400 |
| identification of novel benzoylformate decarboxylases by growth selection. | a growth selection system was established using pseudomonas putida, which can grow on benzaldehyde as the sole carbon source. these bacteria presumably metabolize benzaldehyde via the beta-ketoadipate pathway and were unable to grow in benzoylformate-containing selective medium, but the growth deficiency could be restored by expression in trans of genes encoding benzoylformate decarboxylases. the selection system was used to identify three novel benzoylformate decarboxylases, two of them origina ... | 2006 | 17012586 |
| cultivation-independent examination of horizontal transfer and host range of an incp-1 plasmid among gram-positive and gram-negative bacteria indigenous to the barley rhizosphere. | the host range and transfer frequency of an incp-1 plasmid (pkjk10) among indigenous bacteria in the barley rhizosphere was investigated. a new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16s rrna gene classification was used. indigenous transconjugant rhizosphere bacteria were collected by fluorescence-activated cell sorting and identified by cloning and sequencing of 16s rrna genes from the sorted cells. the host range of th ... | 2006 | 17021220 |
| rapid evolution of diminished transformability in acinetobacter baylyi. | the reason for genetic exchange remains a crucial question in evolutionary biology. acinetobacter baylyi strain adp1 is a highly competent and recombinogenic bacterium. we compared the parallel evolution of wild-type and engineered noncompetent lineages of a. baylyi in the laboratory. if transformability were to result in an evolutionary benefit, it was expected that competent lineages would adapt more rapidly than noncompetent lineages. instead, regardless of competency, lineages adapted to the ... | 2006 | 17028281 |
| [characteristics of war wound infection]. | war wounds are the most complex type of non-targeted injuries due to uncontrolled tissue damage of varied and multifold localizations, exposing sterile body areas to contamination with a huge amount of bacteria. wound contamination is caused by both the host microflora and exogenous agents from the environment (bullets, cloth fragments, dust, dirt, water) due to destruction of the host protective barriers. war wounds are the consequence of destructive effects of various types of projectiles, whi ... | 2006 | 17048790 |
| bacterial identification, clinical significance, and antimicrobial susceptibilities of acinetobacter ursingii and acinetobacter schindleri, two frequently misidentified opportunistic pathogens. | the species belonging to the acinetobacter genus are currently reported as opportunistic pathogens in hospitalized patients with underlying predispositions. however, except for the acinetobacter calcoaceticus-acinetobacter baumannii complex, the identification of other species is frequently unreliable, especially for acinetobacter ursingii and acinetobacter schindleri, newly described in 2001. thus, the clinical significance, phenotypic features, and antimicrobial susceptibilities of these two m ... | 2006 | 17050816 |
| identification of substrates of the mycobacterium tuberculosis proteasome. | the putative proteasome-associated proteins mpa (mycobaterium proteasomal atpase) and pafa (proteasome accessory factor a) of the human pathogen mycobacterium tuberculosis (mtb) are essential for virulence and resistance to nitric oxide. however, a direct link between the proteasome protease and mpa or pafa has never been demonstrated. furthermore, protein degradation by bacterial proteasomes in vitro has not been accomplished, possibly due to the failure to find natural degradation substrates o ... | 2006 | 17082771 |
| identification of rv3230c as the nadph oxidoreductase of a two-protein desa3 acyl-coa desaturase in mycobacterium tuberculosis h37rv. | desa3 is a membrane-bound stearoyl-coa delta(9)-desaturase that produces oleic acid, a precursor of mycobacterial membrane phospholipids and triglycerides. the sequence of desa3 is homologous with those of other membrane desaturases, including the presence of the eight-his motif proposed to bind the diiron center active site. this family of desaturases function as multicomponent complexes and thus require electron transfer proteins for efficient catalytic turnover. here we present evidence that ... | 2006 | 17087501 |
| moxifloxacin in the treatment of skin and skin structure infections. | moxifloxacin is a recent addition to the fluoroquinolone class, differing from ciprofloxacin and other older agents in having much better in vitro activity against gram-positive aerobes while retaining potent activity against gram-negative aerobes. it is also active against the pathogens of human and animal bite wounds and those species of atypical mycobacteria associated with dermatologic infections. its activity against anaerobes is quite variable. moxifloxacin penetrates well into inflammator ... | 2006 | 18360653 |
| spleen and lung involvement by acinetobacter calcoaceticus bacteremia mimicking deep fungal infection in a child with acute non-lymphoblastic leukemia. | 2006 | 16078222 | |
| constitutive expression of catabc genes in the aniline-assimilating bacterium rhodococcus species an-22: production, purification, characterization and gene analysis of cata, catb and catc. | the aniline-assimilating bacterium rhodococcus sp. an-22 was found to constitutively synthesize catb (cis,cis-muconate cycloisomerase) and catc (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported cata (catechol 1,2-dioxygenase). the bacterium maintained the specific activity of the three enzymes at an almost equal level during cultivation on succinate. catb and catc were purified to homogeneity and characterized. catb was a monomer wi ... | 2006 | 16156722 |
| constitutive expression of catabc genes in the aniline-assimilating bacterium rhodococcus species an-22: production, purification, characterization and gene analysis of cata, catb and catc. | the aniline-assimilating bacterium rhodococcus sp. an-22 was found to constitutively synthesize catb (cis,cis-muconate cycloisomerase) and catc (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported cata (catechol 1,2-dioxygenase). the bacterium maintained the specific activity of the three enzymes at an almost equal level during cultivation on succinate. catb and catc were purified to homogeneity and characterized. catb was a monomer wi ... | 2006 | 16156722 |
| a unique polypeptide from the c-terminus of the exocellular esterase of acinetobacter venetianus rag-1 modulates the emulsifying activity of the polymeric bioemulsifier apoemulsan. | an exocellular esterase from the oil-degrading acinetobacter venetianus rag-1 was previously shown to enhance the emulsification and emulsion stabilization properties of the amphipathic, aminopolysaccharide bioemulsifier, emulsan [bach h, berdichevsky y, gutnick d (2003) an exocellular protein from the oil-degrading microbe acinetobacter venetianus rag-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan. appl environ microbiol 69:2608-15]. this enhancement was specific for ... | 2006 | 16237525 |
| isolation and characterization of a phenol-degrading bacterium from an industrial activated sludge. | this paper reports the successful isolation and characterization of a new phenol-degrading bacterium, strain edp3, from activated sludge. strain edp3 is a nonmotile, strictly aerobic, gram-negative, and short-rod or coccobacillary bacterium, which occurs singly, in pairs, or in clusters. 16s rrna gene sequence analysis revealed that strain edp3 belonged to the gamma group of proteobacteria, with a 97.0% identity to 16s rrna gene sequences of acinetobacter calcoaceticus. strain edp3 could aerobic ... | 2006 | 16283294 |
| effect of activated sludge properties and membrane operation conditions on fouling characteristics in membrane bioreactors. | biofouling control is considered to be a major challenge in operating membrane bioreactors (mbrs) for the treatment of wastewater. this study examined the impact of biological, chemical, and physical properties of activated sludge on membrane filtration performance in laboratory-scale mbrs. sludges with different microbial communities were produced using pseudo-continuous stirred-tank reactors and pseudo-plug flow reactors treating a synthetic paper mill wastewater. various filtration resistance ... | 2006 | 16307781 |
| abundant and diverse fungal microbiota in the murine intestine. | enteric microbiota play a variety of roles in intestinal health and disease. while bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. this study utilized a culture-independent method termed oligonucleotide fingerprinting of rrna genes (ofrg) to describe the compositions of fungal and bacterial rrna genes from small and large intestines (tissue and luminal contents) of restricted-flora and specific-pathogen-free mice. ofrg ... | 2006 | 16391120 |
| naturally transformable acinetobacter sp. strain adp1 belongs to the newly described species acinetobacter baylyi. | genotypic and phenotypic analyses were carried out to clarify the taxonomic position of the naturally transformable acinetobacter sp. strain adp1. transfer tdna-pcr fingerprinting, 16s rrna gene sequence analysis, and selective restriction fragment amplification (amplified fragment length polymorphism analysis) indicate that strain adp1 and a second transformable strain, designated 93a2, are members of the newly described species acinetobacter baylyi. transformation assays demonstrate that the a ... | 2006 | 16391138 |
| identification of anthranilate and benzoate metabolic operons of pseudomonas fluorescens and functional characterization of their promoter regions. | in an effort to identify alternate recombinant gene expression systems in pseudomonas fluorescens, we identified genes encoding two native metabolic pathways that were inducible with inexpensive compounds: the anthranilate operon (antabc) and the benzoate operon (benabcd). | 2006 | 16396686 |
| microbiology of airway disease in a cohort of patients with cystic fibrosis. | recent reports document an increasing incidence of new gram-negative pathogens such as stenotrophomonas maltophilia and alcaligenes xylosoxidans isolated from patients with cystic fibrosis, along with an increase in common gram-negative pathogens such as pseudomonas aeruginosa and burkholderia cepacia complex. furthermore, the increase in multidrug-resistance of such organisms makes the therapeutic management of these patients more problematic. therefore, careful isolation and identification, an ... | 2006 | 16405721 |
| nosocomial pneumonia : rationalizing the approach to empirical therapy. | nosocomial pneumonia or hospital-acquired pneumonia (hap) causes considerable morbidity and mortality. it is the second most common nosocomial infection and the leading cause of death from hospital-acquired infections. in 1996 the american thoracic society (ats) published guidelines for empirical therapy of hap. this review focuses on the literature that has appeared since the ats statement. early diagnosis of hap and its etiology is crucial in guiding empirical therapy. since 1996, it has becom ... | 2006 | 16409013 |
| novel organic hydroperoxide-sensing and responding mechanisms for ohrr, a major bacterial sensor and regulator of organic hydroperoxide stress. | xanthomonas campestris pv. phaseoli ohrr belongs to a major family of multiple-cysteine-containing bacterial organic hydroperoxide sensors and transcription repressors. site-directed mutagenesis and subsequent in vivo functional analyses revealed that changing any cysteine residue to serine did not alter the ability of ohrr to bind to the p1 ohrr-ohr promoter but drastically affected the organic hydroperoxide-sensing and response mechanisms of the protein. xanthomonas ohrr requires two cysteine ... | 2006 | 16452421 |
| the thin pili of acinetobacter sp. strain bd413 mediate adhesion to biotic and abiotic surfaces. | two structurally different appendages, thin and thick pili, are found in members of the genus acinetobacter. the presence of pilus structures correlates with different phenotypes, such as adherence to surfaces, a trait not only observed in pathogenic acinetobacter species, as well as motility. however, their distinct individual roles were unknown. to characterize the role of different pili in the physiology of acinetobacter, we isolated the thin pili from the cell surface of acinetobacter sp. st ... | 2006 | 16461692 |
| engineering the genotype of acinetobacter sp. strain adp1 to enhance biosynthesis of cyanophycin. | to study the importance of arginine provision and phosphate limitation for synthesis and accumulation of cyanophycin (cgp) in acinetobacter sp. strain adp1, genes encoding the putative arginine regulatory protein (argr) and the arginine succinyltransferase (asta) were inactivated, and the effects of these mutations on cgp synthesis were analyzed. the inactivation of these genes resulted in a 3.5- or 7-fold increase in cgp content, respectively, when the cells were grown on glutamate. knockout mu ... | 2006 | 16461694 |
| pyrroloquinoline quinone-dependent dehydrogenases from ketogulonicigenium vulgare catalyze the direct conversion of l-sorbosone to l-ascorbic acid. | a novel enzyme, l-sorbosone dehydrogenase 1 (sndh1), which directly converts l-sorbosone to l-ascorbic acid (l-aa), was isolated from ketogulonicigenium vulgare dsm 4025 and characterized. this enzyme was a homooligomer of 75-kda subunits containing pyrroloquinoline quinone (pqq) and heme c as the prosthetic groups. two isozymes of sndh, sndh2 consisting of 75-kda and 55-kda subunits and sndh3 consisting of 55-kda subunits, were also purified from the bacterium. all of the sndhs produced l-aa, a ... | 2006 | 16461703 |
| sequencing of the rpob gene and flanking spacers for molecular identification of acinetobacter species. | acinetobacter species are defined on the basis of several phenotypic characters, results of dna-dna homology, and more recently, similarities or dissimilarities in 16s rrna gene sequences. however, the 16s rrna gene is not polymorphic enough to clearly distinguish all acinetobacter species. we used an rna polymerase beta-subunit gene (rpob)-based identification scheme for the delineation of species within the genus acinetobacter, and towards that end, we determined the complete rpob gene and fla ... | 2006 | 16517861 |
| low-intensity photosensitization may enhance reca production. | three bacterial strains-escherichia coli, acinetobacter calcoaceticus, and the a. calcoaceticus reca- mutant-underwent photosensitization by a low-concentration (0.73 micromol/l) tetramethyl pyridyl porphine (a cationic hydrophylic photosensitizer) and a 4-j/cm2 dose of 407 to 420 nm blue light. the viability of the first two strains decreased by approximately 60%. and that of the reca- strain decreased by 90%. increasing the amount of photosensitizer to 14.6 micromol/l at the same dose of blue ... | 2006 | 16528464 |
| sequence and organization of pmac, an acinetobacter baumannii plasmid harboring genes involved in organic peroxide resistance. | acinetobacter baumannii 19606 harbors pmac, a 9540-bp plasmid that contains 11 predicted open-reading frames (orfs). cloning and transformation experiments using acinetobacter calcoaceticus bd413 mapped replication functions within a region containing four 21-bp direct repeats (ori) and orf 1, which codes for a predicted replication protein. subcloning and tri-parental mating experiments mapped mobilization functions to the product of orf 11 and an adjacent predicted orit. three orfs code for pr ... | 2006 | 16530832 |
| fatty acid and hydroxy acid adaptation in three gram-negative hydrocarbon-degrading bacteria in relation to carbon source. | the lipids of three gram-negative bacteria, acinetobacter calcoaceticus, marinobacter aquaeolei, and pseudomonas oleovorans grown on mineral media supplemented with ammonium acetate or hydrocarbons, were isolated, purified, and their structures determined. three pools of lipids were isolated according to a sequential procedure: unbound lipids extracted with organic solvents, comprising metabolic lipids and the main part of membrane lipids, oh--labile lipids (mainly ester-bound in the lipopolysac ... | 2005 | 16477811 |
| health considerations regarding horizontal transfer of microbial transgenes present in genetically modified crops. | the potential effects of horizontal gene transfer on human health are an important item in the safety assessment of genetically modified organisms. horizontal gene transfer from genetically modified crops to gut microflora most likely occurs with transgenes of microbial origin. the characteristics of microbial transgenes other than antibiotic-resistance genes in market-approved genetically modified crops are reviewed. these characteristics include the microbial source, natural function, function ... | 2005 | 16489267 |
| stereoselective esterase from pseudomonas putida ifo12996 reveals alpha/beta hydrolase folds for d-beta-acetylthioisobutyric acid synthesis. | esterase (est) from pseudomonas putida ifo12996 catalyzes the stereoselective hydrolysis of methyl dl-beta-acetylthioisobutyrate (dl-mati) to produce d-beta-acetylthioisobutyric acid (dat), serving as a key intermediate for the synthesis of angiotensin-converting enzyme inhibitors. the est gene was cloned and expressed in escherichia coli; the recombinant protein is a non-disulfide-linked homotrimer with a monomer molecular weight of 33,000 in both solution and crystalline states, indicating tha ... | 2005 | 16321951 |
| protamylasse, a residual compound of industrial starch production, provides a suitable medium for large-scale cyanophycin production. | protamylasse is a residual compound occurring during the industrial production of starch from potatoes. it contains a variety of nutrients and all necessary minerals and could be used as a carbon, nitrogen, and energy source for the growth of bacteria and also for cyanophycin (cgp) biosynthesis. media containing protamylasse as the sole compound diluted only in water were therefore examined for their suitability in cgp production. among various bacterial strains investigated in this study, a rec ... | 2005 | 16332748 |
| detection and isolation of ultrasmall microorganisms from a 120,000-year-old greenland glacier ice core. | the abundant microbial population in a 3,043-m-deep greenland glacier ice core was dominated by ultrasmall cells (<0.1 microm3) that may represent intrinsically small organisms or starved, minute forms of normal-sized microbes. in order to examine their diversity and obtain isolates, we enriched for ultrasmall psychrophiles by filtering melted ice through filters with different pore sizes, inoculating anaerobic low-nutrient liquid media, and performing successive rounds of filtrations and recult ... | 2005 | 16332755 |
| hydroxylation of the herbicide isoproturon by fungi isolated from agricultural soil. | several asco-, basidio-, and zygomycetes isolated from an agricultural field were shown to be able to hydroxylate the phenylurea herbicide isoproturon [n-(4-isopropylphenyl)-n',n'-dimethylurea] to n-(4-(2-hydroxy-1-methylethyl)phenyl)-n',n'-dimethylurea and n-(4-(1-hydroxy-1-methylethyl)phenyl)-n',n'-dimethylurea. bacterial metabolism of isoproturon has previously been shown to proceed by an initial demethylation to n-(4-isopropylphenyl)-n'-methylurea. in soils, however, hydroxylated metabolites ... | 2005 | 16332769 |
| microscale process evaluation of recombinant biocatalyst libraries: application to baeyer-villiger monooxygenase catalysed lactone synthesis. | microscale processing techniques are rapidly emerging as a cost- effective means for parallel experimentation and hence the evaluation of large libraries of recombinant biocatalysts. in this work, the potential of an automated microscale process is demonstrated in a linked sequence of operations comprising fermentation, enzyme induction and bioconversion using three whole-cell biocatalysts each expressing cyclohexanone monoxygenase (chmo). the biocatalysts, escherichia coli top 10 [pqr239], e. c ... | 2005 | 16208497 |
| multidrug-resistant acinetobacter extremity infections in soldiers. | war wound infection and osteomyelitis caused by multidrug-resistant (mdr) acinetobacter species have been prevalent during the 2003-2005 military operations in iraq. twenty-three soldiers wounded in iraq and subsequently admitted to our facility from march 2003 to may 2004 had wound cultures positive for acinetobacter calcoaceticus-baumannii complex. eighteen had osteomyelitis, 2 burn infection, and 3 deep wound infection. primary therapy for these infections was directed antimicrobial agents fo ... | 2005 | 16102310 |
| streptomyces pharetrae sp. nov., isolated from soil from the semi-arid karoo region. | an actinomycete, strain cza14t, was isolated from worcester in the western cape province of south africa. based on rapid genus identification, 16s-rdna sequence similarities and chemotaxonomy, strain cza14t was identified as a member of the genus streptomyces. it exhibited weak antibiosis against bacillus coagulans atcc 7050t, mycobacterium aurum a+ and acinetobacter calcoaceticus c91. the results of physiological tests and analysis of the 16s-rdna sequence allowed for the differentiation of str ... | 2005 | 16104349 |
| development of a multilocus sequence typing scheme for characterization of clinical isolates of acinetobacter baumannii. | in this study a multilocus sequence typing (mlst) scheme for acinetobacter baumannii was developed and evaluated by using 40 clinical a. baumannii isolates recovered from outbreaks in spanish and german hospitals during the years 1990 to 2001, as well as isolates from other european hospitals and two dsmz reference strains of a. baumannii. for comparison, two isolates of acinetobacter species 13 (sensu tjernberg and ursing), two clinical isolates, and three dsmz strains of a. calcoaceticus (both ... | 2005 | 16145081 |
| increasing stability of water-soluble pqq glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface. | water-soluble quinoprotein glucose dehydrogenase (pqqgdh-b) from acinetobacter calcoaceticus has a great potential for application as a glucose sensor constituent. because this enzyme shows no activity in its monomeric form, correct quaternary structure is essential for the formation of active enzyme. we have previously reported on the increasing of the stability of pqqgdh-b by preventing the subunit dissociation. previous studies were based on decreasing the entropy of quaternary structure diss ... | 2005 | 15715904 |
| epr studies of chlorocatechol 1,2-dioxygenase: evidences of iron reduction during catalysis and of the binding of amphipatic molecules. | chlorocatechol 1,2-dioxygenase from pseudomonas putida (pp 1,2-ccd) is a dioxygenase responsible for ring cleavage during the degradation of recalcitrant aromatic compounds. we determined the zero-field splitting of the fe(iii) cofactor (|d| = 1.3 +/- 0.2 cm(-1)) by electron paramagnetic resonance (epr) experiments that along with other structural data allowed us to infer the fe(iii) coordination environment. the epr spectrum of the ion shows a significantly decrease of the g = 4.3 resonance upo ... | 2005 | 15722436 |
| development of a novel glucose enzyme fuel cell system employing protein engineered pqq glucose dehydrogenase. | glucose dehydrogenase harboring pyrroloquinoline quinone as the prosthetic group (pqqgdh) from acinetobacter calcoaceticus is an ideal enzyme for the anode of biofuel cell, because of its oxygen insensitivity and high catalytic efficiency. however, the application of pqqgdh for the bioanode is inherently limited because of its instability. using ser415cys mutant whose stability was greatly improved, we constructed the biofuel cell system employing the engineered pqqgdh as the bioanode enzyme and ... | 2005 | 15741089 |
| quantitative molecular assay for fingerprinting microbial communities of wastewater and estrogen-degrading consortia. | a quantitative fingerprinting method, called the real-time terminal restriction fragment length polymorphism (real-time-t-rflp) assay, was developed for simultaneous determination of microbial diversity and abundance within a complex community. the real-time-t-rflp assay was developed by incorporating the quantitative feature of real-time pcr and the fingerprinting feature of t-rflp analysis. the assay was validated by using a model microbial community containing three pure strains, an escherich ... | 2005 | 15746346 |
| identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases. | selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (ahs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. first, substrate range mutants of the pseudomonas putida gpo1 and alcanivorax borkumensis ap1 medium-chain-length ahs were obtained by selection experiments with a specially constructed host. in all mutants able to oxidize alkanes longer than c13, w55 (in the case of p. put ... | 2005 | 15601691 |
| impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene. | with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ... | 2005 | 15626648 |
| impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene. | with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ... | 2005 | 15626648 |
| manual and automated instrumentation for identification of enterobacteriaceae and other aerobic gram-negative bacilli. | identification of gram-negative bacilli, both enteric and nonenteric, by conventional methods is not realistic for clinical microbiology laboratories performing routine cultures in today's world. the use of commercial kits, either manual or automated, to identify these organisms is a common practice. the advent of rapid or "spot" testing has eliminated the need for some commonly isolated organisms to be identified with the systems approach. commercially available systems provide more in-depth id ... | 2005 | 15653824 |
| mechanism of lipid-body formation in prokaryotes: how bacteria fatten up. | neutral lipid accumulation is frequently observed in some gram-negative prokaryotes like acinetobacter sp. and most actinomycetes, including the pathogenic mycobacterium tuberculosis and antibiotic producing streptomycetes. we examined the formation of wax ester- and triacylglycerol (tag)-bodies in acinetobacter calcoaceticus and rhodococcus opacus using microscopic, immunological and biophysical methods. a general model for prokaryotic lipid-body formation is proposed, clearly differing from th ... | 2005 | 15661001 |
| thio wax ester biosynthesis utilizing the unspecific bifunctional wax ester synthase/acyl coenzyme a:diacylglycerol acyltransferase of acinetobacter sp. strain adp1. | the bifunctional wax ester synthase/acyl coenzyme a (acyl-coa):diacylglycerol acyltransferase (ws/dgat) from acinetobacter sp. strain adp1 (formerly acinetobacter calcoaceticus adp1) mediating the biosyntheses of wax esters and triacylglycerols was used for the in vivo and in vitro biosynthesis of thio wax esters and dithio wax esters. for in vitro biosynthesis, 5'his(6)ws/dgat comprising an n-terminal his(6) tag was purified from the soluble protein fraction of escherichia coli rosetta(de3)plys ... | 2005 | 15691932 |