Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| morphology and enzymatic degradation of oriented thin film of ultrahigh molecular weight poly[(r)-3-hydroxybutyrate]. | thin films of ultrahigh molecular weight poly[(r)-3-hydroxybutyrate] (p(3hb)) were sheared and isothermally crystallized at 100 degrees c. transmission electron microscopy and atomic force microscopy (afm) observations revealed that thick fibrous textures, on which lamellae are overgrown normal to the long axis of the fibril, run parallel to the shearing direction. a selected area electron diffraction pattern taken from the fibrils exhibits a fiber pattern of p(3hb) alpha-modification, and the c ... | 2004 | 15360288 |
| bioremediation of quinoline-contaminated soil using bioaugmentation in slurry-phase reactor. | to investigate the possibility of using bioaugmentation as a strategy for remediating quinoline-contaminated soil. | 2004 | 15386944 |
| thermophilic, reversible gamma-resorcylate decarboxylase from rhizobium sp. strain mtp-10005: purification, molecular characterization, and expression. | we found the occurrence of thermophilic reversible gamma-resorcylate decarboxylase (gamma-rdc) in the cell extract of a bacterium isolated from natural water, rhizobium sp. strain mtp-10005, and purified the enzyme to homogeneity. the molecular mass of the enzyme was determined to be about 151 kda by gel filtration, and that of the subunit was 37.5 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; in other words, the enzyme was a homotetramer. the enzyme was induced specifically ... | 2004 | 15466039 |
| in-situ atomic force microscopy observation of enzymatic degradation in poly(hydroxyalkanoic acid) thin films: normal and constrained conditions. | the enzymatic degradation of lamellar crystals in poly(hydroxyalkanoic acid) thin films has been visualized by using in-situ dynamic force mode (tapping mode) atomic force microscopy (afm) in buffer solution. it was found that poly(hydroxybutyric acid) (phb) depolymerase from ralstonia pickettii t1 degraded the thin surface layers formed at room temperature first, and that lamellar crystals formed at the crystallization temperature (110 degrees c) were eroded from the crystallographic a-axis to ... | 2004 | 15468218 |
| tour-mediated effector-independent growth phase-dependent activation of the sigma54 ptou promoter of pseudomonas stutzeri ox1. | transcription of the catabolic touabcdef operon, encoding the toluene-o-xylene monooxygenase of pseudomonas stutzeri ox1, is driven by the sigma(54)-dependent ptou promoter, whose activity is controlled by the phenol-responsive ntrc-like activator tour. in this paper we describe for the first time a peculiar characteristic of this system, namely, that ptou transcription is activated in a growth phase-dependent manner in the absence of genuine effectors of the cognate tour regulator. this phenome ... | 2004 | 15489447 |
| [case report: nosocomial ralstonia pickettii infection in neonatal intensive care unit]. | although ralstonia pickettii was known as a saprophytic species, it has been isolated in nosocomial infections in recent years. in this case report, r. pickettii infection of a neonate in the neonatal intensive care unit (icu) was presented. r. pickettii was isolated from the blood cultures of the neonate and also from the incubator and distilled water of the incubator. the isolates revealed identical biochemical and physiological characteristics. the strains were identified by conventional test ... | 2004 | 15490846 |
| diversity of tetracycline resistance genes in bacteria from chilean salmon farms. | twenty-five distinct tetracycline-resistant gram-negative bacteria recovered from four chilean fish farms with no history of recent antibiotic use were examined for the presence of tetracycline resistance (tet) genes. sixty percent of the isolates carried 1 of the 22 known tet genes examined. the distribution was as follows. the tet(a) gene was found in six isolates. the tet(b) gene was found in two isolates, including the first description in the genus brevundimonas: two isolates carried the te ... | 2003 | 12604516 |
| an improved enrichment broth for isolation of escherichia coli o157, with specific reference to starved cells, from radish sprouts. | an enrichment broth was developed for the efficient isolation of escherichia coli o157 from radish sprouts. the broth was buffered peptone water containing 0.5% sodium thioglycolate (stg-bpw), which was designed to allow growth of e. coli o157 in starved and unstarved states. however, this medium suppressed the growth of non-carbohydrate-fermenting obligate aerobes whose colonial appearance on sorbitol macconkey agar containing cefixime and tellurite (ct-smac) resembled that of e. coli o157. bot ... | 2003 | 12620883 |
| characterization of a small plasmid (pmbcp) from bovine pseudomonas pickettii that confers cadmium resistance. | this is the first report of isolation of pseudomonas pickettii from a normal adult bovine duodenum. this organism was one of several bacteria isolated as part of a study to examine cadmium resistance genes (cad(r)) for use in generating transgenic plants to reclaim cadmium-contaminated soils in kansas. p. pickettii containing a plasmid of 2.2kb (designated pmbcp) grew in luria-bertani broth and agar containing up to 800 microm of cadmium chloride and was resistant to 16 antibiotics. curing the o ... | 2003 | 12651180 |
| simple sequence repeats and compositional bias in the bipartite ralstonia solanacearum gmi1000 genome. | ralstonia solanacearum is an important plant pathogen. the genome of r. solananearum gmi1000 is organised into two replicons (a 3.7-mb chromosome and a 2.1-mb megaplasmid) and this bipartite genome structure is characteristic for most r. solanacearum strains. to determine whether the megaplasmid was acquired via recent horizontal gene transfer or is part of an ancestral single chromosome, we compared the abundance, distribution and composition of simple sequence repeats (ssrs) between both repli ... | 2003 | 12697060 |
| removal of phenanthrene from soil by co-cultures of bacteria and fungi pregrown on sugarcane bagasse pith. | sixteen co-cultures composed of four bacteria and four fungi grown on sugarcane bagasse pith were tested for phenanthrene degradation in soil. the four bacteria were identified as pseudomonas aeruginose, ralstonia pickettii, pseudomonas sp. and pseudomonas cepacea. the four fungi were identified as: penicillium sp., trichoderma viride, alternaria tenuis and aspergillus terrus that were previously isolated from different hydrocarbon-contaminated soils. fungi had a statistically significant positi ... | 2003 | 12699938 |
| energy-generating enzymes of burkholderia cepacia and their interactions with macrophages. | we previously demonstrated that several clinical and environmental isolates of burkholderia cepacia secreted atp-utilizing enzymes to the medium; the secretion of these enzymes by cystic fibrosis lung isolate strain 38 was shown to be greatly enhanced in the presence of alpha(2)-macroglobulin. fractionation of the growth medium of cystic fibrosis isolate strain 71 belonging to genomovar i demonstrated the presence of two additional proteins, homologues of pseudomonas aeruginosa azurin and cytoch ... | 2003 | 12730177 |
| microbial population diversity in the urethras of healthy males and males suffering from nonchlamydial, nongonococcal urethritis. | nonchlamydial, nongonococcal urethritis (ncngu) is suggested to be a sexually transmitted disease in men. ncngu patients were compared to control subjects with regard to the presence of potentially infectious bacteria in the first void urine. patients' pre- and post-antibiotic-treatment urine samples and two samples obtained 2 weeks apart from healthy volunteers, who did not receive antibiotic therapy, were analyzed with broad-spectrum pcr tests aiming at eubacterial small subunit rrna genes. re ... | 2003 | 12734237 |
| evaluation of the vitek 2 id-gnb assay for identification of members of the family enterobacteriaceae and other nonenteric gram-negative bacilli and comparison with the vitek gni+ card. | we evaluated the vitek 2 id-gnb identification card (biomérieux, inc., durham, n.c.) for its ability to identify members of the family enterobacteriaceae and other gram-negative bacilli that are isolated in clinical microbiology laboratories. using 482 enteric stock cultures and 103 strains of oxidase-positive, gram-negative glucose-fermenting and nonfermenting bacilli that were maintained at -70 degrees c and passaged three times before use, we inoculated cards according to the manufacturer's d ... | 2003 | 12734254 |
| sepsis, multiple organ failure, and death due to pandoraea pnomenusa infection after lung transplantation. | a 30-year-old man died with pandoraea pnomenusa sepsis after lung transplantation. pandoraea species are gram-negative rods, closely related to, and commonly misidentified as, burkholderia cepacia complex or ralstonia species. heretofore considered soil bacteria and colonizers that infect patients with chronic lung diseases, pandoraea species can produce severe infections. | 2003 | 12734295 |
| purification and properties of an intracellular 3-hydroxybutyrate-oligomer hydrolase (phaz2) in ralstonia eutropha h16 and its identification as a novel intracellular poly(3-hydroxybutyrate) depolymerase. | an intracellular 3-hydroxybutyrate (3hb)-oligomer hydrolase (phaz2(reu)) of ralstonia eutropha was purified from escherichia coli harboring a plasmid containing phaz2(reu). the purified enzyme hydrolyzed linear and cyclic 3hb-oligomers. although it did not degrade crystalline poly(3-hydroxybutyrate) (phb), the purified enzyme degraded artificial amorphous phb at a rate similar to that of the previously identified intracellular phb (iphb) depolymerase (phaz1(reu)). the enzyme appeared to be an en ... | 2003 | 12775684 |
| crystal structure of d-hydantoinase from burkholderia pickettii at a resolution of 2.7 angstroms: insights into the molecular basis of enzyme thermostability. | d-hydantoinase (d-hyd) is an industrial enzyme that is widely used in the production of d-amino acids which are precursors for semisynthesis of antibiotics, peptides, and pesticides. this report describes the crystal structure of d-hydantoinase from burkholderia pickettii (hyd(bp)) at a 2.7-a resolution. the structure of hyd(bp) consists of a core (alpha/beta)(8) triose phosphate isomerase barrel fold and a beta-sheet domain, and the catalytic active site consists of two metal ions and six highl ... | 2003 | 12837777 |
| use of amplified ribosomal dna restriction analysis for identification of ralstonia and pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis. | the recovery of ralstonia and pandoraea species from respiratory tract cultures of patients with cystic fibrosis has recently been reported. these species are difficult to identify, and especially to differentiate from burkholderia cepacia complex organisms, with classical methods. the discriminatory power of amplified ribosomal dna restriction analysis (ardra) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six en ... | 2003 | 12843108 |
| quantitative structure-activity relationship (qsar) analysis of aromatic effector specificity in ntrc-like transcriptional activators from aromatic oxidizing bacteria. | a quantitative structure-activity relationship (qsar) approach was taken to provide mechanistic insights into the interaction between the chemical structure of inducing compounds and the transcriptional activation of aromatic monooxygenase operons among the xylr/dmpr subclass of bacterial ntrc-like transcriptional regulators. compared to xylr and dmpr, a broader spectrum of effector compounds was observed for the tbut system from ralstonia pickettii pko1. the results of qsar analysis for tbut su ... | 2003 | 12855166 |
| substrate inhibition kinetics for toluene and benzene degrading pure cultures and a method for collection and analysis of respirometric data for strongly inhibited cultures. | we present an evaluation of the qualitative and quantitative effects that high concentrations of benzene and toluene have on the growth rate of several pure cultures that use these compounds as their sole carbon and energy source. the cultures employed were five widely studied environmental isolates: pseudomonas putida f1, p. putida mt2, p. mendocina kr, ralstonia pickettii pko1, and burkholderia cepacia g4. three cultures degraded toluene following a pattern consistent with the kinetic model of ... | 2003 | 12889020 |
| classification of ralstonia pickettii-like isolates from the environment and clinical samples as ralstonia insidiosa sp. nov. | thirteen ralstonia pickettii-like isolates from the environment (water, soil and activated sludge) and human clinical samples (including respiratory secretions of cystic fibrosis patients) were investigated in a polyphasic taxonomic study that employed 16s rdna sequence analysis, dna-dna hybridization, determination of dna base composition, whole-cell protein analysis, biochemical characterization and pcr-based assays. all isolates were classified as a novel ralstonia species, for which the name ... | 2003 | 12892129 |
| population pharmacokinetics of ciprofloxacin in pediatric and adolescent patients with acute infections. | the aim of the present study was to characterize the population pharmacokinetics of ciprofloxacin in patients with and without cystic fibrosis ranging in age from 1 day to 24 years and to propose a limited sampling strategy to estimate individual pharmacokinetic parameters. patients were divided into four groups according to the treatment schedule. they received ciprofloxacin by intravenous infusion (30 min) or by the oral route. the number of samples collected from each patient ranged from 1 to ... | 2003 | 14506027 |
| identifying antimicrobial resistance genes with dna microarrays. | we developed and tested a glass-based microarray suitable for detecting multiple tetracycline (tet) resistance genes. microarray probes for 17 tet genes, the beta-lactamase bla(tem-1) gene, and a 16s ribosomal dna gene (escherichia coli) were generated from known controls by pcr. the resulting products (ca. 550 bp) were applied as spots onto epoxy-silane-derivatized, teflon-masked slides by using a robotic spotter. dna was extracted from test strains, biotinylated, hybridized overnight to indivi ... | 2003 | 14506043 |
| rapid screening for freshwater bacterial groups by using reverse line blot hybridization. | the identification of phylogenetic clusters of bacteria that are common in freshwater has provided a basis for probe design to target important freshwater groups. we present a set of 16s ribosomal rna gene-based oligonucleotide probes specific for 15 of these freshwater clusters. the probes were applied in reverse line blot hybridization, a simple method that enables the rapid screening of pcr products from many samples against an array of probes. the optimized assay was made stringent to discri ... | 2003 | 14532039 |
| use of 16s rrna gene sequencing for rapid identification and differentiation of burkholderia pseudomallei and b. mallei. | burkholderia pseudomallei and b. mallei, the causative agents of melioidosis and glanders, respectively, are designated category b biothreat agents. current methods for identifying these organisms rely on their phenotypic characteristics and an extensive set of biochemical reactions. we evaluated the use of 16s rrna gene sequencing to rapidly identify these two species and differentiate them from each other as well as from closely related species and genera such as pandoraea spp., ralstonia spp. ... | 2003 | 14532197 |
| usefulness of rpob gene sequencing for identification of afipia and bosea species, including a strategy for choosing discriminative partial sequences. | bacteria belonging to the genera afipia and bosea are amoeba-resisting bacteria that have been recently reported to colonize hospital water supplies and are suspected of being responsible for intensive care unit-acquired pneumonia. identification of these bacteria is now based on determination of the 16s ribosomal dna sequence. however, the 16s rrna gene is not polymorphic enough to ensure discrimination of species defined by dna-dna relatedness. the complete rpob sequences of 20 strains were fi ... | 2003 | 14602635 |
| efficient degradation of 2,4,6-trichlorophenol requires a set of catabolic genes related to tcp genes from ralstonia eutropha jmp134(pjp4). | 2,4,6-trichlorophenol (2,4,6-tcp) is a hazardous pollutant. several aerobic bacteria are known to degrade this compound. one of these, ralstonia eutropha jmp134(pjp4), a well-known, versatile chloroaromatic compound degrader, is able to grow in 2,4,6-tcp by converting it to 2,6-dichlorohydroquinone, 6-chlorohydroxyquinol, 2-chloromaleylacetate, maleylacetate, and beta-ketoadipate. three enzyme activities encoded by tcp genes, 2,4,6-tcp monooxygenase (tcpa), 6-chlorohydroxyquinol 1,2-dioxygenase ... | 2003 | 14660355 |
| dna fingerprinting of ralstonia paucula by infrequent-restriction-site pcr and randomly amplified polymorphic dna analysis. | ralstonia paucula (formerly cdc group iv c-2) is an environmental organism that can cause serious human infections, occasionally clusters of nosocomial infections. in the present work, 26 strains of r. paucula (4 from the american centers for disease control and prevention collection, 10 from the belgian laboratorium voor microbiologie [lmg] collection, and 12 french clinical isolates) were analyzed with infrequent-restriction-site pcr and randomly amplified polymorphic dna analysis. both techni ... | 2003 | 14662974 |
| outbreak of infusion-related septicemia by ralstonia pickettii in the oncology department. | hospital acquired blood stream infection by ralstonia pickettii in 9 cancer patients related to the heparin solution contamination used to flush the central venous catheter. | 2003 | 14870792 |
| metal ion dependence of recombinant escherichia coli allantoinase. | allantoinase is a suspected dinuclear metalloenzyme that catalyzes the hydrolytic cleavage of the five-member ring of allantoin (5-ureidohydantoin) to form allantoic acid. recombinant escherichia coli allantoinase purified from overproducing cultures amended with 2.5 mm zinc, 1 mm cobalt, or 1 mm nickel ions was found to possess approximately 1.4 zn, 0.0 co, 0.0 ni, and 0.4 fe; 0.1 zn, 1.0 co, 0.0 ni, and 0.2 fe; and 0.0 zn, 0.0 co, 0.6 ni, and 0.1 fe per subunit, respectively, whereas protein o ... | 2003 | 12486048 |
| survival and growth of francisella tularensis in acanthamoeba castellanii. | francisella tularensis is a highly infectious, facultative intracellular bacterium which causes epidemics of tularemia in both humans and mammals at regular intervals. the natural reservoir of the bacterium is largely unknown, although it has been speculated that protozoa may harbor it. to test this hypothesis, acanthamoeba castellanii was cocultured with a strain of f. tularensis engineered to produce green fluorescent protein (gfp) in a nutrient-rich medium. gfp fluorescence within a. castella ... | 2003 | 12514047 |
| n2o-producing microorganisms in the gut of the earthworm aporrectodea caliginosa are indicative of ingested soil bacteria. | the main objectives of this study were (i) to determine if gut wall-associated microorganisms are responsible for the capacity of earthworms to emit nitrous oxide (n(2)o) and (ii) to characterize the n(2)o-producing bacteria of the earthworm gut. the production of n(2)o in the gut of garden soil earthworms (aporrectodea caliginosa) was mostly associated with the gut contents rather than the gut wall. under anoxic conditions, nitrite and n(2)o were transient products when supplemental nitrate was ... | 2003 | 12620856 |
| degradation of mixtures of aromatic and chloroaliphatic hydrocarbons by aromatic hydrocarbon-degrading bacteria. | the well-characterized toluene-oxidizing bacteria pseudomonas putida paw1, p. putida f1, p. mendocina kr1, burkholderia cepacia g4, b. cepacia js150, and ralstonia pickettii pko1, as well as several strains of bacteria isolated from activated sludge, were examined for their ability to degrade mixtures of aromatic and chloroaliphatic hydrocarbons. collectively, the strains tested were able to transform all of the 14 aromatic hydrocarbons included in the mixtures, with most strains degrading five ... | 2003 | 19719688 |
| cloning of an intracellular d(-)-3-hydroxybutyrate-oligomer hydrolase gene from ralstonia eutropha h16 and identification of the active site serine residue by site-directed mutagenesis. | an intracellular d(-)-3-hydroxybutyrate (3hb)-oligomer hydrolase gene from ralstonia eutropha (formerly alcaligenes eutrophus) h16 was cloned, sequenced, and characterized. as a hybridization probe to screen restriction digests of chromosomal dna, an extracellular 3hb-oligomer hydrolase gene from ralstonia pickettii strain (formerly pseudomonas sp. strain) a1 was used. a specific hybridization signal was obtained and a 6.5-kbp smai fragment was cloned in an escherichia coli phagemid vector. the ... | 2002 | 16233278 |
| bacterial chemotaxis toward environmental pollutants: role in bioremediation. | 2002 | 12450797 | |
| ability of the microscan rapid gram-negative id type 3 panel to identify nonenteric glucose-fermenting and nonfermenting gram-negative bacilli. | the microscan rapid neg id3 panel is designed for the identification of enterobacteriaceae and nonenteric glucose-fermenting and nonfermenting gram-negative bacilli. we evaluated this panel for its ability to identify gram-negative non-enterobacteriaceae bacteria. a total of 134 strains, representing 26 genera and 42 species, were taken from storage at -70(o)c, passaged three times before testing, and inoculated into the panels according to the manufacturer's directions before being inserted int ... | 2002 | 12354875 |
| in vitro activities of bal9141, a novel broad-spectrum pyrrolidinone cephalosporin, against gram-negative nonfermenters. | the activities of bal9141 (formerly ro 63-9141), a novel pyrrolidinone-3-ylidenemethyl cephalosporin, against 244 strains of gram-negative nonfermenters were evaluated. the overall mic at which 50% of isolates are inhibited (mic50) and the overall mic90 were 2 and 64 microg/ml, respectively, which are similar to those of imipenem, lower than those of the other cephalosporins tested, amoxicillin, and the ticarcillin-clavulanic acid combination, and much higher than those of ciprofloxacin. bal9141 ... | 2002 | 11850276 |
| biochemical and susceptibility tests useful for identification of nonfermenting gram-negative rods. | six hundred nineteen strains of nonfermenting gram-negative rods were tested for alkaline phosphatase, benzyl-arginine arylamidase, pyrrolidonyl arylamidase, ethylene glycol acidification, and susceptibility to desferrioxamine and colistin. the results were highly discriminant. therefore, the proposed tests may be helpful for the identification of this group of organisms. | 2002 | 11880447 |
| cloning, sequencing, and overexpression in escherichia coli of a phenylserine dehydratase gene from ralstonia pickettii ps22. | the structural gene coding for phenylserine dehydratase from ralstonia pickettii ps22 was cloned into escherichia coli cells, and the nucleotide sequence was identified. the predicted amino acid sequence had high sequence similarity to biodegradative and biosynthetic threonine dehydratases from e. coli and serine dehydratase from human liver. transformed e. coli cells overproduced phenylserine dehydratase, and the recombinant enzyme was purified to homogeneity with a high yield and characterized ... | 2002 | 12596884 |
| directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation. | trichloroethylene (tce) is the most frequently detected groundwater contaminant, and 1-naphthol is an important chemical manufacturing intermediate. directed evolution was used to increase the activity of toluene ortho-monooxygenase (tom) of burkholderia cepacia g4 for both chlorinated ethenes and naphthalene oxidation. when expressed in escherichia coli, the variant tom-green degraded tce (2.5 +/- 0.3 versus 1.39 +/- 0.05 nmol/min/mg of protein), 1,1-dichloroethylene, and trans-dichloroethylene ... | 2002 | 11751810 |
| endotoxin levels in steam and reservoirs of table-top steam sterilizers. | to document endotoxin levels in "statim" cassette sterilizer reservoirs and in steam delivered to the cassette in the unwrapped instrument cycle. to document endotoxin levels in sterilizer reservoir water using different management protocols. | 2002 | 11828908 |
| ralstonia pickettii bacteraemia in a cord blood transplant recipient. | a seven-year old boy with acute lymphoblastic leukemia underwent an hla mismatched cord blood transplant. he developed grade 2 mucositis requiring morphine infusion and grade 3-4 hyperacute graft-versus-host disease affecting the skin, gastrointestinal tract, and liver requiring pulse methylprednisolone. on days 21, 23, and 24 post-transplant, blood culture obtained through the central line and periphery were positive for ralstonia pickettii. the same strain (with the same biochemical profile an ... | 2002 | 11841049 |
| effects of t4 lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors. | rhizosphere bacterial communities of two transgenic potato lines which produce t4 lysozyme for protection against bacterial infections were analyzed in comparison to communities of wild-type plants and transgenic controls not harboring the lysozyme gene. rhizosphere samples were taken from young, flowering, and senescent plants at two field sites in three consecutive years. the communities were characterized in a polyphasic approach. cultivation-dependent methods included heterotrophic plate cou ... | 2002 | 11872484 |
| epidemiology of burkholderia cepacia complex in patients with cystic fibrosis, canada. | the burkholderia cepacia complex is an important group of pathogens in patients with cystic fibrosis (cf). although evidence for patient-to-patient spread is clear, microbial factors facilitating transmission are poorly understood. to identify microbial clones with enhanced transmissibility, we evaluated b. cepacia complex isolates from patients with cf from throughout canada. a total of 905 isolates from the b. cepacia complex were recovered from 447 patients in 8 of the 10 provinces; 369 (83%) ... | 2002 | 11897071 |
| analysis of bacteria contaminating ultrapure water in industrial systems. | bacterial populations inhabiting ultrapure water (upw) systems were investigated. the analyzed upw systems included pilot scale, bench scale, and full size upw plants employed in the semiconductor and other industries. bacteria present in the polishing loop of the upw systems were enumerated by both plate counts and epifluorescence microscopy. assessment of bacterial presence in upw by epifluorescence microscopy (cyanotolyl tetrazolium chloride [ctc] and dapi [4',6'-diamidino-2-phenylindole] sta ... | 2002 | 11916667 |
| development and characterization of a green fluorescent protein-based bacterial biosensor for bioavailable toluene and related compounds. | a green fluorescent protein-based pseudomonas fluorescens strain a506 biosensor was constructed and characterized for its potential to measure benzene, toluene, ethylbenzene, and related compounds in aqueous solutions. the biosensor is based on a plasmid carrying the toluene-benzene utilization (tbu) pathway transcriptional activator tbut from ralstonia pickettii pko1 and a transcriptional fusion of its promoter ptbua1 with a promoterless gfp gene on a broad-host-range promoter probe vector. tbu ... | 2002 | 11916719 |
| comparative evaluation of the bd phoenix and vitek 2 automated instruments for identification of isolates of the burkholderia cepacia complex. | we evaluated two new automated identification systems, the bd phoenix (becton dickinson) and the vitek 2 (biomérieux), for identification of isolates of the burkholderia cepacia complex (bcc). the test sample included 42 isolates of the highly virulent and epidemic genomovar iii, 45 isolates of b. multivorans, and 47 isolates of other members of the bcc. rates of correct identification by the bd phoenix and vitek 2 were similar when all bcc isolates were considered (50 and 53%, respectively) but ... | 2002 | 11980954 |
| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| genetic and biochemical characterization of a 2,4,6-trichlorophenol degradation pathway in ralstonia eutropha jmp134. | ralstonia eutropha jmp134 can grow on several chlorinated aromatic pollutants, including 2,4-dichlorophenoxyacetate and 2,4,6-trichlorophenol (2,4,6-tcp). although a 2,4,6-tcp degradation pathway in jmp134 has been proposed, the enzymes and genes responsible for 2,4,6-tcp degradation have not been characterized. in this study, we found that 2,4,6-tcp degradation by jmp134 was inducible by 2,4,6-tcp and subject to catabolic repression by glutamate. we detected 2,4,6-tcp-degrading activities in jm ... | 2002 | 12057943 |
| ralstonia pickettii traced in blood culture bottles. | over a 9-month period, 14 strains of ralstonia pickettii were isolated from various biological samples inoculated in a blood culture medium. molecular epidemiological investigation confirmed the relatedness of the strains. the source of the contamination proved to be the blood culture bottle caps. | 2002 | 12089303 |
| infection by ralstonia species in cystic fibrosis patients: identification of r. pickettii and r. mannitolilytica by polymerase chain reaction. | the frequency of respiratory tract infections caused by ralstonia species in persons with cystic fibrosis (cf) and the role of these species in cf pulmonary disease are not well documented. in part, this lack of documentation may be attributed to the difficulty in accurately identifying ralstonia species; r. mannitolilytica and r. pickettii in particular may be misidentified as other closely related species, particularly those of the burkholderia cepacia complex. we used polyphasic analyses to i ... | 2002 | 12095436 |
| origins of the 2,4-dinitrotoluene pathway. | the degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. previous work defined the steps in 2,4-dinitrotoluene (2,4-dnt) metabolism through the ring fission reaction. the results presented here characterize subsequent steps in the pathway that yield the central metabolic intermediates pyruvate and propionyl coenzyme a (coa). the genes encoding the degradative pathway were identified within a 27-kb region of dna cloned ... | 2002 | 12107140 |
| microbial degradation of quinoline by immobilized cells of burkholderia pickettii. | a quinoline-biodegrading microorganism was isolated from activated sludge of coke-oven wastewater treatment plant using quinoline as sole carbon and nitrogen source. it is a gram negative, rod-shaped and aerobic strain, which was identified as burkholderia pickettii. the biodegradation of quinoline was carried out with this isolated strain. analysis by high performance liquid chromatography and gas chromatography/mass spectrum (gc/ms) revealed that 2-hydroxyquinoline (2-oh-q) was the first inter ... | 2002 | 12108721 |
| method for qualifying microbial removal performance of 0.1 micron rated filters. part iv: retention of hydrogenophaga pseudoflava (atcc 700892) and ralstonia pickettii (atcc 700591) by 0.2 and 0.22 micron rated filters. | ralstonia pickettii has emerged as a bioburden microorganism of considerable importance in pharmaceutical processes utilizing conventional 0.2 or 0.22 micron rated "sterilizing grade" filters. in this article, we re-evaluated and studied the retention efficiencies of 0.2 micron rated nylon 6.6 and 0.22 microns rated modified polyvinylidene fluoride (pvdf) filters for hydrogenophaga pseudoflava (atcc 700892) and r. pickettii (atcc 700591). out of a total of forty-four 0.2/0.22 micron rated filter ... | 2002 | 12109335 |
| culture-based and non-growth-dependent detection of the burkholderia cepacia complex in soil environments. | burkholderia cepacia complex (bcc) bacteria reside in soil, plant rhizospheres, and water, but their prevalence and distribution in outdoor environments is not clear. we sampled a variety of soil and rhizosphere environments with which people may have contact: playgrounds, athletic fields, parks, hiking trails, residential yards, and gardens. a total of 91 sites was sampled in three large u.s. cities. in the first phase of the study, putative bcc isolates were recovered on burkholderia cepacia s ... | 2002 | 12147469 |
| [cloning, sequencing and high expression in escherichia coli of d-hydantoinase gene from burkholderia pickettii]. | a strain, mmr003, used for d-p-hpg production in industry was classified as burkholderia pickettii by morphological observation and biochemical characterization. the gene encoding the d-hydantoinase enzyme was cloned, sequenced and expressed in escherichia coli. the nucleotide sequence of the 5.0 kb insert of subclone pxz-total was determined. one open reading frame of 1374 bp was found and predicted to encode a polypeptide consisting of 458 amino acids in size of 50 kd. the amino acid sequence ... | 2002 | 12148274 |
| survival and nutritional requirements of three bacteria isolated from ultrapure water. | bacteria isolated previously from ultrapure water (upw) systems were examined for their ability to survive in upw, with the ultimate goal of elucidating potential carbon and energy sources for the bacteria. two strains of ralstonia pickettii isolated from different areas within the upw system (pretreatment and polishing loop, and referred to as strains 3a1 and mf254a, respectively) and a strain of bradyrhizobium sp. were compared to increase our understanding of the fundamental behavior of bacte ... | 2002 | 12161774 |
| direct pcr detection of burkholderia cepacia complex and identification of its genomovars by using sputum as source of dna. | we developed a nested pcr assay that detects the reca gene of the burkholderia cepacia complex in sputum. the product of the first pcr round is also used to identify the genomovar of the pathogen. the protocol achieves high sensitivity and specificity with simple interpretation of genomovar status. | 2002 | 12202599 |
| enzymatic hydrolysis of chemosynthesized atactic poly(3-hydroxybutyrate) by poly(3-hydroxyalkanoate) depolymerase from acidovorax sp. tp4 and ralstonia pickettii t1. | the enzymatic degradability of chemosynthesized atactic poly([r,s]-3-hydroxybutyrate) [a-p(3hb)] by two types of extracellular poly(3-hydroxyalkanoate) (pha) depolymerases purified from ralstonia pickettii t1 (phaz(ral)) and acidovorax sp. tp4 (phaz(aci)), defined respectively as pha depolymerase types i and ii according to the position of the lipase box in the catalytic domain, were studied. the enzymatic degradation of a-p(3hb) by phaz(aci) depolymerase was confirmed from the results of weight ... | 2002 | 12217032 |
| a new modified ortho cleavage pathway of 3-chlorocatechol degradation by rhodococcus opacus 1cp: genetic and biochemical evidence. | the 4-chloro- and 2,4-dichlorophenol-degrading strain rhodococcus opacus 1cp has previously been shown to acquire, during prolonged adaptation, the ability to mineralize 2-chlorophenol. in addition, homogeneous chlorocatechol 1,2-dioxygenase from 2-chlorophenol-grown biomass has shown relatively high activity towards 3-chlorocatechol. based on sequences of the n terminus and tryptic peptides of this enzyme, degenerate pcr primers were now designed and used for cloning of the respective gene from ... | 2002 | 12218013 |
| microbial origin of glutamate, hibernation and tissue trauma: an in vivo microdialysis study. | using quantitative microdialysis in hibernating arctic ground squirrels (ags), striatal glutamate concentrations ([glu](dia)) progressively increased to approximately 200 microm after 3 days of microdialysis in euthermic but not hibernating ground squirrels. initially, the progressive increase in [glu](dia) was thought to be related to greater tissue response in euthermic animals. alternatively, given the vastly different body temperatures between the two groups (37 vs. 3 degrees c), glutamate m ... | 2002 | 12323415 |
| carbon and hydrogen stable isotope fractionation during aerobic bacterial degradation of aromatic hydrocarbons. | 13c/(12)c and d/h stable isotope fractionation during aerobic degradation was determined for pseudomonas putida strain mt-2, pseudomonas putida strain f1, ralstonia pickettii strain pko1, and pseudomonas putida strain ncib 9816 grown with toluene, xylenes, and naphthalene. different types of initial reactions used by the respective bacterial strains could be linked with certain extents of stable isotope fractionation during substrate degradation. | 2002 | 12324375 |
| use of 16s rrna gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center. | during 1999, we used partial 16s rrna gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. these 46 isolates were genotypically identified as pseudomonas aeruginosa (19 isolates, 12 patients), achromobacter xylosoxidans (10 isolates, 8 ... | 2002 | 12354883 |
| cloning of the class d beta-lactamase gene from burkholderia pseudomallei and studies on its expression in ceftazidime-susceptible and -resistant strains. | ceftazidime is the antibiotic of choice for the treatment of melioidosis. ceftazidime-resistant burkholderia pseudomallei have been identified and beta-lactamase production implicated in resistance. in this study, 25 strains of b. pseudomallei (15 clinical and 10 environmental strains) were examined for their ability to yield mutants that overexpress beta-lactamase. ceftazidime-resistant mutants were selected readily at high frequency and displayed four- to eight-fold increases in the mics of ce ... | 2002 | 12356787 |
| characterization of the adaptive response to trichloroethylene-mediated stresses in ralstonia pickettii pko1. | in ralstonia pickettii pko1, a denitrifying toluene oxidizer that carries a toluene-3-monooxygenase (t3mo) pathway, the biodegradation of toluene and trichloroethylene (tce) by the organism is induced by tce at high concentrations. in this study, the effect of tce preexposure was studied in the context of bacterial protective response to tce-mediated toxicity in this organism. the results of tce degradation experiments showed that cells induced by tce at 110 mg/liter were more tolerant to tce-me ... | 2002 | 12406709 |
| change of surface structure of poly(3-hydroxybutyrate) film upon enzymatic hydrolysis by phb depolymerase. | the change in the surface structure of poly[(r)-3-hydroxybutyrate] [phb] films upon the enzymatic hydrolysis was analyzed by attenuated total reflection infrared [atr/ir] spectrometry. as enzymes, phb depolymerases isolated from ralstonia pickettii t1 and pseudomonas stutzeri were used. by curve decomposition of the carbonyl stretching band of atr/ir spectra, the change in the surface crystallinity of phb films by exposure to buffer containing 0, 1, and 4 microg of phb depolymerases was estimate ... | 2002 | 12425671 |
| cross-regulation between a novel two-component signal transduction system for catabolism of toluene in pseudomonas mendocina and the todst system from pseudomonas putida. | the tmoabcdef genes encode the toluene-4-monooxygenase from pseudomonas mendocina kr1. upstream from the tmoa gene an open reading frame, tmox, encoding a protein 83% identical to todx (todx being the initial gene in the todxfc1c2badegih operon from pseudomonas putida dot-t1e) was found. the tmox gene is also the initial gene in the tmoxabcdef gene cluster. the transcription initiation point from the tmox promoter was mapped, and the sequence upstream revealed striking identity with the promoter ... | 2002 | 12446657 |
| detection and activity of insertion sequences in environmental strains of burkholderia. | the presence of two insertion sequences, is406 and is407, was tested by polymerase chain reaction (pcr) amplification in 25 strains representing 15 burkholderia species and the close relative ralstonia pickettii. a total of 50% of the 25 strains contained at least one of the two insertion sequences (iss) and a statistically significant correlation was found between the occurrences of is406 and is407. moreover, pcr-rflp studies of the amplified fragments showed that is406 is largely conserved amo ... | 2001 | 11846770 |
| cloning of an intracellular poly[d(-)-3-hydroxybutyrate] depolymerase gene from ralstonia eutropha h16 and characterization of the gene product. | an intracellular poly[d(-)-3-hydroxybutyrate] (phb) depolymerase gene (phaz) has been cloned from ralstonia eutropha h16 by the shotgun method, sequenced, and characterized. nucleotide sequence analysis of a 2.3-kbp dna fragment revealed an open reading frame of 1,260 bp, encoding a protein of 419 amino acids with a predicted molecular mass of 47,316 da. the crude extract of escherichia coli containing the phb depolymerase gene digested artificial amorphous phb granules and released mainly oligo ... | 2001 | 11114905 |
| transcriptional organization and dynamic expression of the hbpcad genes, which encode the first three enzymes for 2-hydroxybiphenyl degradation in pseudomonas azelaica hbp1. | pseudomonas azelaica hbp1 degrades the toxic substance 2-hydroxybiphenyl (2-hbp) by means of three enzymes that are encoded by structural genes hbpc, hbpa, and hbpd. these three genes form a small noncontiguous cluster. their expression is activated by the product of regulatory gene hbpr, which is located directly upstream of the hbpcad genes. the hbpr protein is a transcription activator and belongs to the so-called xylr/dmpr subclass within the ntrc family of transcriptional activators. transc ... | 2001 | 11114926 |
| phzo, a gene for biosynthesis of 2-hydroxylated phenazine compounds in pseudomonas aureofaciens 30-84. | certain strains of root-colonizing fluorescent pseudomonas spp. produce phenazines, a class of antifungal metabolites that can provide protection against various soilborne root pathogens. despite the fact that the phenazine biosynthetic locus is highly conserved among fluorescent pseudomonas spp., individual strains differ in the range of phenazine compounds they produce. this study focuses on the ability of pseudomonas aureofaciens 30-84 to produce 2-hydroxyphenazine-1-carboxylic acid (2-oh-pca ... | 2001 | 11114932 |
| role of the dmpr-mediated regulatory circuit in bacterial biodegradation properties in methylphenol-amended soils. | pathway substrates and some structural analogues directly activate the regulatory protein dmpr to promote transcription of the dmp operon genes encoding the (methyl)phenol degradative pathway of pseudomonas sp. strain cf600. while a wide range of phenols can activate dmpr, the location and nature of substituents on the basic phenolic ring can limit the level of activation and thus utilization of some compounds as assessed by growth on plates. here we address the role of the aromatic effector res ... | 2001 | 11133441 |
| ralstonia paucula (formerly cdc group iv c-2): unsuccessful strain differentiation with pcr-based methods, study of the 16s-23s spacer of the rrna operon, and comparison with other ralstonia species (r. eutropha, r. pickettii, r. gilardii, and r. solanacearum). | ralstonia paucula (formerly cdc group iv c-2) can cause serious human infections. confronted in 1995 with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic dna analysis was poorly discriminatory. in this study, we used pcr-ribotyping and pcr-restriction fragment length polymorphism analysis of the spacer 16s-23s ribosomal dna (rdna); both methods were unable to differentiate r. pa ... | 2001 | 11136807 |
| phylogenetic and functional heterogeneity of sediment biofilms along environmental gradients in a glacial stream. | we used in situ hybridization with fluorescently labeled rrna-targeted oligonucleotide probes concurrently with measurements of bacterial carbon production, biomass, and extracellular polymeric substances (eps) to describe the bacterial community in sediments along a glacial stream. the abundance of sediment-associated archaea, as detected with the arch915 probe, decreased downstream of the glacier snout, and a major storm increased their relative abundance by a factor of 5.5 to 7.9. bacteria of ... | 2001 | 11157246 |
| nickel-resistance-based minitransposons: new tools for genetic manipulation of environmental bacteria. | the ncc and nre nickel resistance determinants from ralstonia eutropha-like strain 31a were used to construct mini-tn5 transposons. broad host expression of nickel resistance was observed for the nre minitransposons in members of the alpha, beta, and gamma subclasses of the proteobacteria, while the ncc minitransposons expressed nickel resistance only in r. eutropha-like strains. | 2001 | 11157282 |
| cloning and characterization of the pnb genes, encoding enzymes for 4-nitrobenzoate catabolism in pseudomonas putida tw3. | pseudomonas putida strain tw3 is able to metabolize 4-nitrotoluene via 4-nitrobenzoate (4nben) and 3, 4-dihydroxybenzoic acid (protocatechuate [pca]) to central metabolites. we have cloned, sequenced, and characterized a 6-kbp fragment of tw3 dna which contains five genes, two of which encode the enzymes involved in the catabolism of 4nben to pca. in order, they encode a 4nben reductase (pnba) which is responsible for catalyzing the direct reduction of 4nben to 4-hydroxylaminobenzoate with the o ... | 2001 | 11157934 |
| recurrent achromobacter piechaudii bacteremia in a patient with hematological malignancy. | we describe a recurrent bacteremia caused by achromobacter (formerly alcaligenes) piechaudii in association with an intravenous catheter in an immunocompromised 73-year-old man. this is the first reported case of bacteremia due to a. piechaudii. | 2001 | 11158159 |
| an active role for a structured b-linker in effector control of the sigma54-dependent regulator dmpr. | the activities of many prokaryotic sigma54-dependent transcriptional activators are controlled by the n-terminal a-domain of the protein, which is linked to the central transcriptional activation domain via a short b-linker. it used to be thought that these b-linkers simply serve as flexible tethers. here we show that the b-linker of the aromatic-responsive regulator dmpr and many other regulators of the family contain signature heptad repeats with regularly spaced hydrophobic amino acids. mutan ... | 2001 | 11179226 |
| the black cat/white cat principle of signal integration in bacterial promoters. | 2001 | 11226149 | |
| phenotypic methods for determining genomovar status of the burkholderia cepacia complex. | recent taxonomic advances have demonstrated that burkholderia cepacia is a cluster of at least seven closely related genomic species (or genomovars) collectively referred to as the b. cepacia complex, all of which may cause infections among cystic fibrosis patients and other vulnerable individuals. thus, it is important for clinical microbiologists to be able to differentiate genomovars. prior to this study, 361 b. cepacia complex isolates and 51 isolates easily confused with b. cepacia complex ... | 2001 | 11230429 |
| melamine deaminase and atrazine chlorohydrolase: 98 percent identical but functionally different. | the gene encoding melamine deaminase (tria) from pseudomonas sp. strain nrrl b-12227 was identified, cloned into escherichia coli, sequenced, and expressed for in vitro study of enzyme activity. melamine deaminase displaced two of the three amino groups from melamine, producing ammeline and ammelide as sequential products. the first deamination reaction occurred more than 10 times faster than the second. ammelide did not inhibit the first or second deamination reaction, suggesting that the lower ... | 2001 | 11274097 |
| map of the incp1beta plasmid ptsa encoding the widespread genes (tsa) for p-toluenesulfonate degradation in comamonas testosteroni t-2. | the catabolic incp1beta plasmid ptsa from comamonas testosteroni t-2 was mapped by subtractive analysis of restriction digests, by sequencing outwards from the tsa operon (toluenesulfonate degradation), and by generating overlapping, long-distance-pcr amplification products. the plasmid was estimated to comprise 72 +/- 4 kb. the tsa region was found to be a composite transposon flanked by two is1071 elements. a cryptic tsa operon was also present in the tsa transposon. those backbone genes and r ... | 2001 | 11282598 |
| solution structure of the toluene 4-monooxygenase effector protein (t4mod). | toluene 4-monooxygenase (t4mo) from pseudomonas mendocina catalyzes the nadh- and o(2)-dependent hydroxylation of toluene to form p-cresol. the complex consists of an nadh oxidoreductase (t4mof), a rieske ferredoxin (t4moc), a diiron hydroxylase [t4moh, with (alphabetagamma)(2) quaternary structure], and a catalytic effector protein (t4mod). the solution structure of the 102-amino acid t4mod effector protein has been determined from 2d and 3d (1)h, (13)c, and (15)n nmr spectroscopic data. the st ... | 2001 | 11297417 |
| [avoidable "fever of unknown origin" due to ralstonia pickettii bacteremia]. | ralstonia pickettii bacteraemia from the atrial part of a ventriculo-atrial shunt was diagnosed as the cause of recurring febrile episodes with systemic toxicity in a 38-year old female. a prior neurosurgical resection of intracerebral cavernoma was complicated by postoperative recurrent meningitis with this non-fermenting gram-negative rod, due to an intra-operative contamination of a in situ shunt, from which only the intracerebral part of the ventriculo-atriostomy had been removed, following ... | 2001 | 11307484 |
| classification of ralstonia pickettii biovar 3/'thomasii' strains (pickett 1994) and of new isolates related to nosocomial recurrent meningitis as ralstonia mannitolytica sp. nov. | strains isolated independently from two patients could be recognized as ralstonia pickettii biovar 3/'thomasii'. the 16s rdna sequences of these strains and two other strains of r. pickettii biovar 3/'thomasii' clustered at less than 98% similarity versus all other described ralstonia species and at less than 97 % versus the two other r. pickettii biovars. the separate species status of r. pickettii biovar 3/'thomasii' was confirmed by dna-dna hybridization, indicating less than 60% dna homology ... | 2001 | 11321101 |
| assignment of cdc weak oxidizer group 2 (wo-2) to the genus pandoraea and characterization of three new pandoraea genomospecies. | cdc weak oxidizer group 2 (wo-2) consists of nine phenotypically similar human clinical isolates received by the centers for disease control and prevention between 1989 and 1998. four of the isolates were from blood, three were from sputum, and one each was from bronchial fluid and maxillary sinus. all are aerobic nonfermentative, motile gram-negative rods with one to eight polar flagella per cell. all grew at 25 and 35 degrees c and were positive for catalase, urease (usually delayed 3 to 7 day ... | 2001 | 11325997 |
| [quinoline removal in fluidized-bed bioreactor using immobilized cells]. | burkholderia pickettii, capable of using quinoline as sole source of carbon, nitrogen and energy, was immobilized by an innovative pva-h3bo3-gauze method. a fluidized-bed bioreactor, in which immobilized cells were added, was constructed to degrade quinoline in wastewater. when the initial concentrations of quinoline were 100, 350 and 500 mg/l, the bioreactor can remove it completely within 2.5, 6 and 12 hours respectively. a series of zero-order reaction equations were proposed to describe quin ... | 2001 | 11382050 |
| plasmid and chromosomal basis of tolerance to cadmium and resistance to antibiotics in normal bovine duodenal bacterial flora. | cadmium (cd) tolerance and antibiotic resistance was studied in duodenal flora of 20 normal bovine samples. twelve bacterial isolates (5 staphylococcus spp, 4 enterococcus faecalis, 2 bacillus spp, and a pseudomonas sp) were grown in luria broth containing 0.05 to 0.8 mm of cadmium chloride (cdcl). all isolates displayed multiple antibiotic resistance, with 2 enterococcus strains and pseudomonas pickettii demonstrating resistance to 12/17 antibiotics tested. with the exception of staphylococcus ... | 2001 | 11383651 |
| purification and catalytic properties of the chlorophenol 4-monooxygenase from burkholderia cepacia strain ac1100. | burkholderia cepacia strain ac1100 can be induced for the degradation of 2,4,5-trichlorophenol (2,4,5-tcp). we have purified the active enzyme 30-fold to apparent homogeneity with a 44% yield by a two-step chromatographic procedure, and showed that it consists of a single type of subunit of 59 kda based on sds-page using coomassie blue and sypro staining. this enzyme has no bound prosthetic group but requires exogenous addition of fad and nadh to perform the dioxygen-dependent hydroxylation in t ... | 2001 | 11410285 |
| phcs represses gratuitous expression of phenol-metabolizing enzymes in comamonas testosteroni r5. | we identified an open reading frame, designated phcs, downstream of the transcriptional activator gene (phcr) for the expression of multicomponent phenol hydroxylase (mph) in comamonas testosteroni r5. the deduced product of phcs was homologous to aphs of c. testosteroni ta441, which belongs to the gntr family of transcriptional regulators. the transformation of pseudomonas aeruginosa pao1c (phenol negative, catechol positive) with pror502 containing phcr and the mph genes conferred the ability ... | 2001 | 11418563 |
| pseudomonas pickettii (biovar va-ii): a rare cause of bacteremias in haematologic patients. | 2001 | 11422418 | |
| organization and regulation of meta cleavage pathway genes for toluene and o-xylene derivative degradation in pseudomonas stutzeri ox1. | pseudomonas stutzeri ox1 meta pathway genes for toluene and o-xylene catabolism were analyzed, and loci encoding phenol hydroxylase, catechol 2,3-dioxygenase, 2-hydroxymuconate semialdehyde dehydrogenase, and 2-hydroxymuconate semialdehyde hydrolase were mapped. phenol hydroxylase converted a broad range of substrates, as it was also able to transform the nongrowth substrates 2,4-dimethylphenol and 2,5-dimethylphenol into 3,5-dimethylcatechol and 3,6-dimethylcatechol, respectively, which, howeve ... | 2001 | 11425758 |
| evaluation of vitek 2 rapid identification and susceptibility testing system against gram-negative clinical isolates. | a total of 281 strains of miscellaneous members of the family enterobacteriaceae, pseudomonas aeruginosa, and other gram-negative bacteria were evaluated by use of identification tests with the vitek 2 system (biomérieux) and an api identification system (biomérieux). a total of 237 (95%) strains were correctly identified to the species level. only six (2.1%) strains were misidentified, and eight (2.8%) strains were not identified. among 14 strains with discrepant identifications, 8 (57.1%) stra ... | 2001 | 11474023 |
| isolation of an insertion sequence from ralstonia solanacearum race 1 and its potential use for strain characterization and detection. | a new insertion sequence (is), is1405, was isolated and characterized from a ralstonia solanacearum race 1 strain by the method of insertional inactivation of the sacb gene. sequence analysis indicated that the is is closely related to the members of is5 family, but the extent of nucleotide sequence identity in 5' and 3' noncoding regions between is1405 and other members of is5 family is only 23 to 31%. nucleotide sequences of these regions were used to design specific oligonucleotide primers fo ... | 2001 | 11525989 |
| identification of strains of alcaligenes and agrobacterium by a polyphasic approach. | the number of stable discriminant biochemical characters is limited in the genera alcaligenes and agrobacterium, whose species are consequently difficult to distinguish from one another by conventional tests. moreover, genomic studies have recently drastically modified the nomenclature of these genera; for example, alcaligenes xylosoxidans was transferred to the genus achromobacter in 1998. twenty-five strains of achromobacter xylosoxidans, three strains of an agrobacterium sp., five strains of ... | 2001 | 11526136 |
| infection of acanthamoeba polyphaga with simkania negevensis and s. negevensis survival within amoebal cysts. | simkania negevensis, a novel microorganism belonging to the family simkaniaceae in the order chlamydiales, has an intracellular developmental cycle during which two morphological entities, elementary bodies (eb) and reticulate bodies (rb), are seen by electron microscopy. rates of seropositivity to the organism are high in certain population groups, and s. negevensis has been associated with respiratory illness in humans. this study reports for the first time the ability of s. negevensis to surv ... | 2001 | 11571186 |
| genetic and functional analysis of the tbc operons for catabolism of alkyl- and chloroaromatic compounds in burkholderia sp. strain js150. | burkholderia sp. strain js150 is able to metabolize a wide range of alkyl-and chloroaromatic hydrocarbons through multiple, apparently redundant catabolic pathways. previous research has shown that strain js150 is able to synthesize enzymes for multiple upper pathways as well as multiple lower pathways to accommodate variously substituted catechols that result from degradation of complex mixtures of monoaromatic compounds. we report here the genetic organization and functional characterization o ... | 2001 | 11571188 |
| taxonomy and identification of the burkholderia cepacia complex. | 2001 | 11574551 | |
| influence of substrate exposure history on biodegradation in a porous medium. | this study investigates the influence of fluctuating toluene concentrations on aerobic toluene degradation in a sandy porous medium colonized with ralstonia pickettii pko1. column effluent toluene concentrations were found to increase after a temporary decrease in influent toluene concentration. subsequent examination of the spatial gradient of toluene degradative activity in the column suggested that the observed increase in effluent toluene concentrations was attributable to an adverse effect ... | 2001 | 11588828 |
| bacteria and archaea physically associated with gulf of mexico gas hydrates. | although there is significant interest in the potential interactions of microbes with gas hydrate, no direct physical association between them has been demonstrated. we examined several intact samples of naturally occurring gas hydrate from the gulf of mexico for evidence of microbes. all samples were collected from anaerobic hemipelagic mud within the gas hydrate stability zone, at water depths in the ca. 540- to 2,000-m range. the delta(13)c of hydrate-bound methane varied from -45.1 per thous ... | 2001 | 11679338 |