Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| mutations and genomic islands can explain the strain dependency of sugar utilization in 21 strains of propionibacterium freudenreichii. | propionibacterium freudenreichii (pf) is an actinobacterium used in cheese technology and for its probiotic properties. pf is also extremely adaptable to several ecological niches and can grow on a variety of carbon and nitrogen sources. the aim of this work was to discover the genetic basis for strain-dependent traits related to its ability to use specific carbon sources. high-throughput sequencing technologies were ideal for this purpose as they have the potential to decipher genomic diversity ... | 2015 | 25886522 |
| immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from brucella melitensis. | brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. immunity against brucella melitensis involves antigen-specific cd4(+) and cd8(+) t-cells activation and humoral immune responses. due to negative aspects of live attenuated vaccines, much attention has been focused on finding brucella-protective antigens to introduce them as potential subunit vaccine candidates. | 2015 | 26019797 |
| apoptotic-like leishmania exploit the host's autophagy machinery to reduce t-cell-mediated parasite elimination. | apoptosis is a well-defined cellular process in which a cell dies, characterized by cell shrinkage and dna fragmentation. in parasites like leishmania, the process of apoptosis-like cell death has been described. moreover upon infection, the apoptotic-like population is essential for disease development, in part by silencing host phagocytes. nevertheless, the exact mechanism of how apoptosis in unicellular organisms may support infectivity remains unclear. therefore we investigated the fate of a ... | 2015 | 25801301 |
| modeling, molecular dynamics, and docking assessment of transcription factor rho: a potential drug target in brucella melitensis 16m. | the zoonotic disease brucellosis, a chronic condition in humans affecting renal and cardiac systems and causing osteoarthritis, is caused by brucella, a genus of gram-negative, facultative, intracellular pathogens. the mode of transmission and the virulence of the pathogens are still enigmatic. transcription regulatory elements, such as rho proteins, play an important role in the termination of transcription and/or the selection of genes in brucella. adverse effects of the transcription inhibito ... | 2015 | 25848225 |
| 3-way networks: application of hypergraphs for modelling increased complexity in comparative genomics. | we present and develop the theory of 3-way networks, a type of hypergraph in which each edge models relationships between triplets of objects as opposed to pairs of objects as done by standard network models. we explore approaches of how to prune these 3-way networks, illustrate their utility in comparative genomics and demonstrate how they find relationships which would be missed by standard 2-way network models using a phylogenomic dataset of 211 bacterial genomes. | 2015 | 25815802 |
| immune responses of bison and efficacy after booster vaccination with brucella abortus strain rb51. | thirty-one bison heifers were randomly assigned to receive saline or a single vaccination with 10(10) cfu of brucella abortus strain rb51. some vaccinated bison were randomly selected for booster vaccination with rb51 at 11 months after the initial vaccination. mean antibody responses to rb51 were greater (p < 0.05) in vaccinated bison after initial and booster vaccination than in nonvaccinated bison. the proliferative responses by peripheral blood mononuclear cells (pbmc) from the vaccinated bi ... | 2015 | 25673305 |
| the role of autophagy in intracellular pathogen nutrient acquisition. | following entry into host cells intracellular pathogens must simultaneously evade innate host defense mechanisms and acquire energy and anabolic substrates from the nutrient-limited intracellular environment. most of the potential intracellular nutrient sources are stored within complex macromolecules that are not immediately accessible by intracellular pathogens. to obtain nutrients for proliferation, intracellular pathogens must compete with the host cell for newly-imported simple nutrients or ... | 2015 | 26106587 |
| regulation of iron metabolism by hepcidin under conditions of inflammation. | iron is a redox-active metal required as a cofactor in multiple metalloproteins essential for a host of life processes. the metal is highly toxic when present in excess and must be strictly regulated to prevent tissue and organ damage. hepcidin, a molecule first characterized as an antimicrobial peptide, plays a critical role in the regulation of iron homeostasis. multiple stimuli positively influence the expression of hepcidin, including iron, inflammation, and infection by pathogens. in this m ... | 2015 | 26055723 |
| comparative genomic analysis of brucella abortus vaccine strain 104m reveals a set of candidate genes associated with its virulence attenuation. | the brucella abortus strain 104m, a spontaneously attenuated strain, has been used as a vaccine strain in humans against brucellosis for 6 decades in china. despite many studies, the molecular mechanisms that cause the attenuation are still unclear. here, we determined the whole-genome sequence of 104m and conducted a comprehensive comparative analysis against the whole genome sequences of the virulent strain, a13334, and other reference strains. this analysis revealed a highly similar genome st ... | 2015 | 26039674 |
| roles of reactive oxygen species-degrading enzymes of francisella tularensis schu s4. | francisella tularensis is a facultative intracellular bacterium utilizing macrophages as its primary intracellular habitat and is therefore highly capable of resisting the effects of reactive oxygen species (ros), potent mediators of the bactericidal activity of macrophages. we investigated the roles of enzymes presumed to be important for protection against ros. four mutants of the highly virulent schu s4 strain with deletions of the genes encoding catalase (katg), glutathione peroxidase (gpx), ... | 2015 | 25802058 |
| identification of a novel small non-coding rna modulating the intracellular survival of brucella melitensis. | bacterial small non-coding rnas (srnas) are gene expression modulators respond to environmental changes, stressful conditions, and pathogenesis. in this study, by using a combined bioinformatic and experimental approach, eight novel srna genes were identified in intracellular pathogen brucella melitensis. bsr0602, one srna that was highly induced in stationary phase, was further examined and found to modulate the intracellular survival of b. melitensis. bsr0602 was present at very high levels in ... | 2015 | 25852653 |
| biochemical and spectroscopic properties of brucella microti glutamate decarboxylase, a key component of the glutamate-dependent acid resistance system. | in orally acquired bacteria, the ability to counteract extreme acid stress (ph ⩽ 2.5) ensures survival during transit through the animal host stomach. in several neutralophilic bacteria, the glutamate-dependent acid resistance system (gdar) is the most efficient molecular system in conferring protection from acid stress. in escherichia coli its structural components are either of the two glutamate decarboxylase isoforms (gada, gadb) and the antiporter, gadc, which imports glutamate and exports γ ... | 2015 | 25853037 |
| thermodynamic matchers for the construction of the cuckoo rna family. | rna family models describe classes of functionally related, non-coding rnas based on sequence and structure conservation. the most important method for modeling rna families is the use of covariance models, which are stochastic models that serve in the discovery of yet unknown, homologous rnas. however, the performance of covariance models in finding remote homologs is poor for rna families with high sequence conservation, while for families with high structure but low sequence conservation, the ... | 2015 | 25779873 |
| concordance and discordance of sequence survey methods for molecular epidemiology. | the post-genomic era is characterized by the direct acquisition and analysis of genomic data with many applications, including the enhancement of the understanding of microbial epidemiology and pathology. however, there are a number of molecular approaches to survey pathogen diversity, and the impact of these different approaches on parameter estimation and inference are not entirely clear. we sequenced whole genomes of bacterial pathogens, burkholderia pseudomallei, yersinia pestis, and brucell ... | 2015 | 25737810 |
| prevalence of and factors associated with brucella sero-positivity in cattle in urban and peri-urban gulu and soroti towns of uganda. | brucellosis is a key zoonosis of major public health, animal welfare and economic significance, and is endemic in livestock in uganda. a cross-sectional epidemiological study was carried out to estimate the sero-prevalence of brucellosis and identify factors associated with sero-positivity in cattle in urban and peri-urban gulu and soroti towns of northern and eastern uganda, respectively. a total of 1007 sera and data on biologically plausible risk factors from 166 herds and their spatial locat ... | 2015 | 25716482 |
| structure and function of a short lov protein from the marine phototrophic bacterium dinoroseobacter shibae. | light, oxygen, voltage (lov) domains are widely distributed in plants, algae, fungi, bacteria, and represent the photo-responsive domains of various blue-light photoreceptor proteins. their photocycle involves the blue-light triggered adduct formation between the c(4a) atom of a non-covalently bound flavin chromophore and the sulfur atom of a conserved cysteine in the lov sensor domain. lov proteins show considerable variation in the structure of n- and c-terminal elements which flank the lov co ... | 2015 | 25887755 |
| exploring genetic polymorphism in innate immune genes in indian cattle (bos indicus) and buffalo (bubalus bubalis) using next generation sequencing technology. | activation of innate immunity initiates various cascades of reactions that largely contribute to defense against physical, microbial or chemical damage, prompt for damage repair and removal of causative organisms as well as restoration of tissue homeostasis. genetic polymorphism in innate immune genes plays prominent role in disease resistance capabilities in various breeds of cattle and buffalo. here we studied single nucleotide variations (snp/snv) and haplotype structure in innate immune gene ... | 2015 | 26925373 |
| giant thyroid abscess related to postpartum brucella infection. | thyroid gland infection, although rare, may be a life threatening disease. thyroid abscess, arising from acute suppurative thyroiditis (ast), is a rare clinic condition depending on widespread use of antibiotics. infection may involve one or both lobes and abscess formation may not be apparent until late stage of the progress of illness. thyroid left lobe is more often affected than the right one. brucellosis, especially obvious in endemic areas, is a widely seen zoonosis around the world. altho ... | 2015 | 25861492 |
| functional genomic screen identifies klebsiella pneumoniae factors implicated in blocking nuclear factor κb (nf-κb) signaling. | klebsiella pneumoniae is an etiologic agent of community-acquired and nosocomial pneumonia. it has been shown that k. pneumoniae infections are characterized by reduced early inflammatory response. recently our group has shown that k. pneumoniae dampens the activation of inflammatory responses by antagonizing the activation of the nf-κb canonical pathway. our results revealed that k. pneumoniae capsule polysaccharide (cps) was necessary but not sufficient to attenuate inflammation. to identify a ... | 2015 | 25971969 |
| a nad-dependent glutamate dehydrogenase coordinates metabolism with cell division in caulobacter crescentus. | coupling cell cycle with nutrient availability is a crucial process for all living cells. but how bacteria control cell division according to metabolic supplies remains poorly understood. here, we describe a molecular mechanism that coordinates central metabolism with cell division in the α-proteobacterium caulobacter crescentus. this mechanism involves the nad-dependent glutamate dehydrogenase gdhz and the oxidoreductase-like kido. while enzymatically active gdhz directly interferes with ftsz p ... | 2015 | 25953831 |
| draft genome sequence of brucella abortus virulent strain 544. | here, we present the draft genome sequence and annotation of brucella abortus virulent strain 544. the genome of this strain is 3,289,405 bp long, with 57.2% g+c content. a total of 3,259 protein-coding genes and 60 rna genes were predicted. | 2015 | 25953161 |
| commensal streptococcus salivarius modulates pparγ transcriptional activity in human intestinal epithelial cells. | the impact of commensal bacteria in eukaryotic transcriptional regulation has increasingly been demonstrated over the last decades. a multitude of studies have shown direct effects of commensal bacteria from local transcriptional activity to systemic impact. the commensal bacterium streptococcus salivarius is one of the early bacteria colonizing the oral and gut mucosal surfaces. it has been shown to down-regulate nuclear transcription factor (nf-кb) in human intestinal cells, a central regulato ... | 2015 | 25946041 |
| brucella abortus induces the premature death of human neutrophils through the action of its lipopolysaccharide. | most bacterial infections induce the activation of polymorphonuclear neutrophils (pmns), enhance their microbicidal function, and promote the survival of these leukocytes for protracted periods of time. brucella abortus is a stealthy pathogen that evades innate immunity, barely activates pmns, and resists the killing mechanisms of these phagocytes. intriguing clinical signs observed during brucellosis are the low numbers of brucella infected pmns in the target organs and neutropenia in a proport ... | 2015 | 25946018 |
| cervical lymph nodes as a selective niche for brucella during oral infections. | cervical lymph nodes (cln) are the first lymph nodes encountered by material taking the oral route. to study their role in orally acquired infections, we analyzed 307 patients of up to 14 years treated in the university clinic of skopje, macedonia, for brucellosis, a zoonotic bacterial disease frequently acquired by ingestion of contaminated dairy products. from these children, 36% had lymphadenopathy. among orally infected children, lymphadenopathy with cln being the only lymph nodes affected w ... | 2015 | 25919005 |
| genomic and proteomic evidences unravel the uv-resistome of the poly-extremophile acinetobacter sp. ver3. | ultraviolet radiation can damage biomolecules, with detrimental or even lethal effects for life. even though lower wavelengths are filtered by the ozone layer, a significant amount of harmful uv-b and uv-a radiation reach earth's surface, particularly in high altitude environments. high-altitude andean lakes (haals) are a group of disperse shallow lakes and salterns, located at the dry central andes region in south america at altitudes above 3,000 m. as it is considered one of the highest uv-exp ... | 2015 | 25954258 |
| immunomodulation and anti-inflammatory effects of garlic compounds. | the benefits of garlic to health have been proclaimed for centuries; however, only recently have allium sativum and its derivatives been proposed as promising candidates for maintaining the homeostasis of the immune system. the complex biochemistry of garlic makes it possible for variations in processing to yield different preparations with differences in final composition and compound proportion. in this review, we assess the most recent experimental results, which indicate that garlic appears ... | 2015 | 25961060 |
| detection of leptospira spp. and brucella abortus antibodies in free-living jaguars (panthera onca) in two protected areas of northern pantanal, brazil. | this study aimed to assess the exposure of free-living jaguars (panthera onca) to leptospira spp. and brucella abortus in two conservation units in the pantanal of mato grosso, brazil. the presence of antibodies in blood samples of eleven jaguars was investigated using autochthonous antigens isolated in brazil added to reference antigen collection applied to diagnosis of leptospirosis by microscopic agglutination test (mat). the rose bengal test was applied for b. abortus antibodies. two (18.2%) ... | 2015 | 25923900 |
| brucella suis vaccine strain s2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic er-stress. | brucella, which is regarded as an intracellular pathogen responsible for a zoonotic disease called brucellosis, survives and proliferates within several types of phagocytic and non-phagocytic cells. brucella infects not only their preferred hosts but also other domestic and wild animal species, inducing abortion and infertility. therefore, the interaction between uterine cells and brucella is important for understanding the pathogenesis of this disease. in this study, we describe the brucella su ... | 2015 | 25633898 |
| immunoproteomic identification of immunodominant antigens independent of the time of infection in brucella abortus 2308-challenged cattle. | brucellosis is a vital zoonotic disease caused by brucella, which infects a wide range of animals and humans. accurate diagnosis and reliable vaccination can control brucellosis in domestic animals. this study examined novel immunogenic proteins that can be used to detect brucella abortus infection or as an effective subcellular vaccine. in an immunoproteomic assay, 55 immunodominant proteins from b. abortus 544 were observed using two dimensional electrophoresis (2de) and immunoblot profiles wi ... | 2015 | 25885057 |
| molecular detection of ehrlichia chaffeensis in humans, costa rica. | 2015 | 25695614 | |
| moonlighting o-acetylserine sulfhydrylase: new functions for an old protein. | o-acetylserine sulfhydrylase a (cysk) is the pyridoxal 5'-phosphate-dependent enzyme that catalyzes the final reaction of cysteine biosynthesis in bacteria. cysk was initially identified in a complex with serine acetyltransferase (cyse), which catalyzes the penultimate reaction in the synthetic pathway. this "cysteine synthase" complex is stabilized by insertion of the cyse c-terminus into the active-site of cysk. remarkably, the cysk/cyse binding interaction is conserved in most bacterial and p ... | 2015 | 25731080 |
| immunization with brucella virb proteins reduces organ colonization in mice through a th1-type immune response and elicits a similar immune response in dogs. | virb proteins from brucella spp. constitute the type iv secretion system, a key virulence factor mediating the intracellular survival of these bacteria. here, we assessed whether a th1-type immune response against virb proteins may protect mice from brucella infection and whether this response can be induced in the dog, a natural host for brucella. splenocytes from mice immunized with virb7 or virb9 responded to their respective antigens with significant and specific production of gamma interfer ... | 2015 | 25540276 |
| isolation and molecular characterization of brucella isolates in cattle milk in uganda. | brucellosis is endemic in livestock and humans in uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. to shed new light on the epidemiology of brucellosis in uganda the present study used phenotypic and molecular approaches to delineate the brucella species, biovars, and genotypes shed in cattle milk. brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in uganda. these isolates had ... | 2015 | 25793204 |
| full genome snp-based phylogenetic analysis reveals the origin and global spread of brucella melitensis. | brucellosis is an important zoonotic disease that affects both humans and animals. we sequenced the full genome and characterised the genetic diversity of two brucella melitensis isolates from malaysia and the philippines. in addition, we performed a comparative whole-genome single nucleotide polymorphism (snp) analysis of b. melitensis strains collected from around the world, to investigate the potential origin and the history of the global spread of b. melitensis. | 2015 | 25888205 |
| decreased in vivo virulence and altered gene expression by a brucella melitensis light-sensing histidine kinase mutant. | brucella species utilize diverse virulence factors. previously, brucella abortus light-sensing histidine kinase was identified as important for cellular infection. here, we demonstrate that a brucella melitensis lov-hk (bm-lov-hk) mutant strain has strikingly different gene expression than wild type. general stress response genes including the alternative sigma factor rpoe1 and its anti-anti-sigma factor phyr were downregulated, while flagellar, quorum sensing (qs), and type iv secretion system ... | 2015 | 25132657 |
| coordinated zinc homeostasis is essential for the wild-type virulence of brucella abortus. | metal homeostasis in bacterial cells is a highly regulated process requiring intricately coordinated import and export, as well as precise sensing of intracellular metal concentrations. the uptake of zinc (zn) has been linked to the virulence of brucella abortus; however, the capacity of brucella strains to sense zn levels and subsequently coordinate zn homeostasis has not been described. here, we show that expression of the genes encoding the zinc uptake system znuabc is negatively regulated by ... | 2015 | 25691532 |
| botulinum toxin a complex exploits intestinal m cells to enter the host and exert neurotoxicity. | to cause food-borne botulism, botulinum neurotoxin (bont) in the gastrointestinal lumen must traverse the intestinal epithelial barrier. however, the mechanism by which bont crosses the intestinal epithelial barrier remains unclear. bonts are produced along with one or more non-toxic components, with which they form progenitor toxin complexes (ptcs). here we show that serotype a1 l-ptc, which has high oral toxicity and makes the predominant contribution to causing illness, breaches the intestina ... | 2015 | 25687350 |
| unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry. | glucans are polymers of d-glucose with differing linkages in linear or branched sequences. they are constituents of microbial and plant cell-walls and involved in important bio-recognition processes, including immunomodulation, anticancer activities, pathogen virulence, and plant cell-wall biodegradation. translational possibilities for these activities in medicine and biotechnology are considerable. high-throughput micro-methods are needed to screen proteins for recognition of specific glucan s ... | 2015 | 25670804 |
| two-tiered histidine kinase pathway involved in heat shock and salt sensing in the general stress response of sphingomonas melonis fr1. | the general stress response (gsr) allows bacteria to monitor and defend against a broad set of unrelated, adverse environmental conditions. in alphaproteobacteria, the key step in gsr activation is phosphorylation of the response regulator phyr. in sphingomonas melonis fr1, seven phyr-activating kinases (paks), paka to pakg, are thought to directly phosphorylate phyr under different stress conditions, but the nature of the activating signals remains obscure. pakf, a major sensor of nacl and heat ... | 2015 | 25666137 |
| brucella cβg induces a dual pro- and anti-inflammatory response leading to a transient neutrophil recruitment. | brucella is the causing agent of a chronic zoonosis called brucellosis. the brucella β-1,2 cyclic glucan (cβg) is a virulence factor, which has been described as a potent immune stimulator, albeit with no toxicity for cells and animals. we first used a genome-wide approach to characterize human myeloid dendritic cell (mdc) responses to cβg. transcripts related to inflammation (il-6, il2ra, ptgs2), chemokine (cxcr7, cxcl2) and anti-inflammatory pathways (tnfaip6, socs3) were highly expressed in c ... | 2015 | 25654761 |
| improved serodiagnosis of bovine brucellosis by novel synthetic oligosaccharide antigens representing the capping m epitope elements of brucella o-polysaccharide. | members of the genus brucella have cell wall characteristics of gram-negative bacteria, which in the most significant species includes o-polysaccharide (ops). serology is the most cost-effective means of detecting brucellosis, as infection with smooth strains of brucella leads to the induction of high antibody titers against the ops, an unbranched homopolymer of 4,6-dideoxy-4-formamido-d-mannopyranosyl residues (d-rha4nfo) that are variably α(1→2)- and α(1→3)-linked. six d-rha4nfo homo-oligosacc ... | 2015 | 25653412 |
| foodborne outbreak of human brucellosis caused by ingested raw materials of fetal calf on jeju island. | since the first reported case of human brucellosis in 2002 in south korea, its incidence has been increasing nationally. however, bovine brucellosis has not been present from 2005 to date on jeju island. despite jeju island being considered a clean area for bovine brucellosis, we experienced an outbreak of human brucellosis between 2012 and 2013. herein, we report cases with human brucellosis after ingestion of raw materials of fetal calf at a restaurant. patients were identified by isolation of ... | 2015 | 25510725 |
| interferon γ-induced gtpase promotes invasion of listeria monocytogenes into trophoblast giant cells. | listeria monocytogenes is well known for having the ability to cross the placental barrier, leading to fetal infections and abortion. however, the mechanisms leading to infectious abortion are poorly understood. in this study, we demonstrate that interferon γ-induced gtpase (igtp) contributes to the invasion of l. monocytogenes into trophoblast giant (tg) cells, which are placental immune cells. knockdown of igtp in tg cells decreased the relative efficiencies of l. monocytogenes invasion. moreo ... | 2015 | 25645570 |
| mutant brucella abortus membrane fusogenic protein induces protection against challenge infection in mice. | brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. the mechanism of virulence of brucella spp. is not yet fully understood. therefore, it is crucial to identify new molecules that serve as virulence factors to better understand this host-pathogen interplay. here, we evaluated the role of the brucella membrane fusogenic protein (mfp) and outer membrane protein 19 (omp19) in bacterial pathogenesis. in this ... | 2015 | 25644010 |
| brucella endocarditis as a late onset complication of brucellosis. | brucella endocarditis (be) is a rare but life threatening complication of brucellosis. we present a case report of a patient with relapsing brucellosis complicated with aortic valve endocarditis. the patient underwent valve replacement and required prolonged antibiotic treatment because of rupture of the noncoronary leaflet and development of congestive heart failure. since the onset of endocarditis in patients with brucellosis is not known, proper follow-up is required in order to identify any ... | 2015 | 25713740 |
| the future of biologics: applications for food allergy. | allergic diseases affect millions worldwide, with growing evidence of an increase in allergy occurrence over the past few decades. current treatments for allergy include corticosteroids to reduce inflammation and allergen immunotherapy; however, some subjects experience treatment-resistant inflammation or adverse reactions to these treatments, and there are currently no approved therapeutics for the treatment of food allergy. there is a dire need for new therapeutic approaches for patients with ... | 2015 | 25662303 |
| genetic analysis of signal integration by the sinorhizobium meliloti sensor kinase feuq. | two-component signalling systems allow bacteria to recognize and respond to diverse environmental stimuli. auxiliary proteins can provide an additional layer of control to these systems. the sinorhizobium meliloti feupq two-component system is required for symbiotic development and is negatively regulated by the auxiliary small periplasmic protein feun. this study explores the mechanistic basis of this regulation. we provide evidence that feun directly interacts with the sensor kinase feuq. isol ... | 2015 | 25479839 |
| adrenal steroids modulate the immune response during brucella abortus infection by a mechanism that depends on the regulation of cytokine production. | human brucellosis is a protean disease with a diversity of clinical signs and symptoms resulting from infection with brucella species. recent reports suggest a cross-regulation between adrenal steroids (cortisol and dehydroepiandrosterone [dhea]) and the immune system. monocytes and macrophages are the main replication niche for brucella. therefore, we investigated the role of adrenal hormones on the modulation of the immune response mediated by macrophages in b. abortus infection. cortisol trea ... | 2015 | 25733519 |
| polyionic vaccine adjuvants: another look at aluminum salts and polyelectrolytes. | adjuvants improve the adaptive immune response to a vaccine antigen by modulating innate immunity or facilitating transport and presentation. the selection of an appropriate adjuvant has become vital as new vaccines trend toward narrower composition, expanded application, and improved safety. functionally, adjuvants act directly or indirectly on antigen presenting cells (apcs) including dendritic cells (dcs) and are perceived as having molecular patterns associated either with pathogen invasion ... | 2015 | 25648619 |
| circulating strains of brucella abortus in cattle in santo domingo de los tsáchilas province - ecuador. | the province of santo domingo de los tsáchilas in ecuador represents the largest informal cattle market. because of its strategic position, cattle movement is very high and therefore we selected this region, to determine the strain variation of brucella sp. part of the study aimed at the isolation, biotyping, and genotyping of brucella species from milk and supra-mammary lymph nodes of sero-positive bovines, using selective farrell medium, biochemical assays, and is711-pcr, amos-pcr, and hoof-pr ... | 2015 | 25806363 |
| hfq plays important roles in virulence and stress adaptation in cronobacter sakazakii atcc 29544. | cronobacter spp. are opportunistic pathogens that cause neonatal meningitis and sepsis with high mortality in neonates. despite the peril associated with cronobacter infection, the mechanisms of pathogenesis are still being unraveled. hfq, which is known as an rna chaperone, participates in the interaction with bacterial small rnas (srnas) to regulate posttranscriptionally the expression of various genes. recent studies have demonstrated that hfq contributes to the pathogenesis of numerous speci ... | 2015 | 25754196 |
| yip1a, a novel host factor for the activation of the ire1 pathway of the unfolded protein response during brucella infection. | brucella species replicate within host cells in the form of endoplasmic reticulum (er)-derived vacuoles. the mechanisms by which the bacteria are sequestered into such vacuoles and obtain a continuous membrane supply for their replication remain to be elucidated. in the present study, we provided several lines of evidence that demonstrate the mechanism by which b. abortus acquires the er-derived membrane. first, during brucella infection, the ire1 pathway, but not the perk and atf6 pathways, of ... | 2015 | 25742138 |
| interaction network and localization of brucella abortus membrane proteins involved in the synthesis, transport, and succinylation of cyclic β-1,2-glucans. | cyclic β-1,2-glucans (cβg) are periplasmic homopolysaccharides that play an important role in the virulence and interaction of brucella with the host. once synthesized in the cytoplasm by the cβg synthase (cgs), cβg are transported to the periplasm by the cβg transporter (cgt) and succinylated by the cβg modifier enzyme (cgm). here, we used a bacterial two-hybrid system and coimmunoprecipitation techniques to study the interaction network between these three integral inner membrane proteins. our ... | 2015 | 25733613 |
| biotechnological applications of functional metagenomics in the food and pharmaceutical industries. | microorganisms are found throughout nature, thriving in a vast range of environmental conditions. the majority of them are unculturable or difficult to culture by traditional methods. metagenomics enables the study of all microorganisms, regardless of whether they can be cultured or not, through the analysis of genomic data obtained directly from an environmental sample, providing knowledge of the species present, and allowing the extraction of information regarding the functionality of microbia ... | 2015 | 26175729 |
| crystal structure of cyclic nucleotide-binding-like protein from brucella abortus. | the cyclic nucleotide-binding (cnb)-like protein (cnb-l) from brucella abortus shares sequence homology with cnb domain-containing proteins. we determined the crystal structure of cnb-l at 2.0 å resolution in the absence of its c-terminal helix and nucleotide. the 3d structure of cnb-l is in a two-fold symmetric form. each protomer shows high structure similarity to that of cgmp-binding domain-containing proteins, and likely mimics their nucleotide-free conformation. a key residue, glu17, mediat ... | 2015 | 26549229 |
| brucella abortus cell cycle and infection are coordinated. | brucellae are facultative intracellular pathogens. the recent development of methods and genetically engineered strains allowed the description of cell-cycle progression of brucella abortus, including unipolar growth and the ordered initiation of chromosomal replication. b. abortus cell-cycle progression is coordinated with intracellular trafficking in the endosomal compartments. bacteria are first blocked at the g1 stage, growth and chromosome replication being resumed shortly before reaching t ... | 2015 | 26497941 |
| the history of brucellosis in the pacific island countries and territories and its re-emergence. | there are few publications on brucellosis within the pacific island countries and territories (picts). the reason is possibly because the cattle population has been reportedly free of the disease for many years until a re-emergence occurred in the fiji islands (viti levu) in 2009. this paper reports on the outbreak of brucellosis in fiji and its progression between 2009 and 2013 in the context of an overview of brucellosis in the pacific island community. review of the literature found only 28 a ... | 2015 | 26497272 |
| serological diagnosis of bovine brucellosis using b. melitensis strain b115. | bovine brucellosis is diagnosed by official tests, such as rose bengal plate test (rbpt) and complement fixation test (cft). both tests detect antibodies directed against the lipolysaccharide (lps) of brucella cell wall. despite their good sensitivity, those tests do not discriminate between true positive and false positive serological reactions (fpsr), the latter being generated by animals infected with other gram negative microorganisms that share components of brucella lps. in this study, an ... | 2015 | 26476137 |
| influence of brucella abortus lipopolysaccharide as an adjuvant on the immunogenicity of hpv-16 l1vlp vaccine in mice. | brucella abortus lipopolysaccharide (lps) has less toxicity and no pyrogenic properties in comparison with other bacterial lps. it is a toll-like receptor 4 agonist and has been shown to have the potential use as a vaccine adjuvant. in this study, the immunostimulatory properties of lps from smooth and rough strains of b. abortus (s19 and rb51) as adjuvants were investigated for the human papillomavirus type 16 (hpv16) l1 virus-like particles (l1vlps) vaccines. c57bl/6 mice were immunized subcut ... | 2015 | 25187406 |
| express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level. | an immunochromatographic test system was developed for rapid detection of the levels of specific igg antibodies to brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. the pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. the results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme assay and are ... | 2015 | 24579979 |
| snapshots of conformational changes shed light into the ntrx receiver domain signal transduction mechanism. | brucella abortus is an important pathogenic bacterium that has to overcome oxygen deficiency in order to achieve a successful infection. previously, we proved that a two-component system formed by the histidine kinase ntry and the response regulator ntrx is essential to achieve an adaptive response to low oxygen tension conditions. even though the relevance of this signaling pathway has already been demonstrated in other microorganisms, its molecular activation mechanism has not yet been describ ... | 2015 | 26113057 |
| proteomics-based identification of immunodominant proteins of brucellae using sera from infected hosts points towards enhanced pathogen survival during the infection. | brucella (b.) species lack classical virulence factors, but escape effectively the immune response of the host. the species brucella abortus and brucella melitensis infect predominantly cattle and small ruminants such as sheep or goats, respectively, but account also for most human cases. these two species share remarkably similar genomes but different proteomes have been demonstrated. this might be one of the reasons for their host specificity. a comprehensive identification of immunodominant p ... | 2015 | 25446124 |
| subcutaneous immunization with a novel immunogenic candidate (urease) confers protection against brucella abortus and brucella melitensis infections. | brucellosis is a world prevalent endemic illness that is transmitted from domestic animals to humans. brucella spp. exploits urease for survival in the harsh conditions of stomach during the gastrointestinal infection. in this study, we examined the immune response and the protection elicited by using recombinant brucella urease (rurease) vaccination in balb/c mice. the urease gene was cloned in pet28a and the resulting recombinant protein was employed as subunit vaccine. recombinant protein was ... | 2015 | 25939375 |
| recombinant l7/l12 protein entrapping plga (poly lactide-co-glycolide) micro particles protect balb/c mice against the virulent b. abortus 544 infection. | brucella abortus is the etiologic agent of brucellosis, a zoonotic infection affecting a wide range of animals. it is a highly infectious disease of pandemic potential reporting over 500,000 new human cases annually. till date, there is no reported vaccine for humans and the available animal vaccines are unsafe, therefore a safe and effective subunit vaccine is highly sought for. in this study, we have evaluated rl7/l12 protein encapsulated in microparticles of plga (85:15), a biocompatible and ... | 2015 | 25930114 |
| the effects of red ginseng saponin fraction-a (rgsf-a) on phagocytosis and intracellular signaling in brucella abortus infected raw 264.7 cells. | this study indicated that rgsf-a caused a marked reduction in the adherence, internalization and intracellular growth of brucella abortus in rgsf-a-treated cells. furthermore, a decline in the intensity of f-actin fluorescence was observed in rgsf-a-treated cells compared with untreated b. abortus-infected cells. in addition, an evaluation of phagocytic signaling proteins by western blot analysis revealed an apparent reduction of erk and p38α phosphorylation levels in b. abortus-infected rgsf-a- ... | 2015 | 25926528 |
| intermediate rough brucella abortus s19δper mutant is diva enable, safe to pregnant guinea pigs and confers protection to mice. | brucella abortus s19 is a smooth strain used as live vaccine against bovine brucellosis. smooth lipopolysaccharide (lps) is responsible for its residual virulence and serological interference. rough mutants defective of lps are more attenuated but confers lower level of protection. we describe a modified b. abortus s19 strain, named as s19δper, which exhibits intermediate rough phenotype with residual o-polysaccharide (ops). deletion of perosamine synthetase gene resulted in substantial attenuat ... | 2015 | 25869887 |
| rapid and specific identification of brucella abortus using the loop-mediated isothermal amplification (lamp) assay. | a rapid and accurate diagnosis of brucellosis is required to reduce and prevent the spread of disease among animals and the risk of transfer to humans. in this study, a brucella abortus-specific (ba) lamp assay was developed, that had six primers designed from the bruab2_0168 region of chromosome i. the specificity of this lamp assay was confirmed with brucella reference strains, b. abortus vaccine strains, b. abortus isolates and phylogenetically or serologically related strains. the detection ... | 2015 | 25841288 |
| development of a dual vaccine for prevention of brucella abortus infection and escherichia coli o157:h7 intestinal colonization. | zoonoses that affect human and animal health have an important economic impact. in the study now presented, a bivalent vaccine has been developed that has the potential for preventing the transmission from cattle to humans of two bacterial pathogens: brucella abortus and shiga toxin-producing escherichia coli (stec). a 66kda chimeric antigen, composed by espa, intimin, tir, and h7 flagellin (eith7) from stec, was constructed and expressed in b. abortus δpgm vaccine strain (babδpgm). mice orally ... | 2015 | 25820069 |
| immunogenicity and protective effect of recombinant brucella abortus ndk (rndk) against a virulent strain b. abortus 544 infection in balb/c mice. | in this study, we particularly evaluated the protective effect of recombinant protein encoded by brucella abortus 544 ndk (nucleoside diphosphate kinase) gene against b. abortus infection in the balb/c mice. cloning and expression of b. abortus ndk was accomplished by pcr amplification into a pmal expression system, and purification of a recombinant ndk (rndk). as for the determination of igg responses, rndk induced vigorous igg production, especially higher in igg2a compared to igg1 with titers ... | 2015 | 25724777 |
| vaccination with recombinant l7/l12-truncated omp31 protein induces protection against brucella infection in balb/c mice. | brucellosis is the most common bacterial zoonotic disease worldwide and no vaccine is available for the prevention of human brucellosis. in humans, brucellosis is mostly caused by brucella melitensis and brucella abortus. the outer membrane protein 31 (omp31) and l7/l12 are immunodominant and protective antigens conserved in human brucella pathogens. in the present study, we evaluated the humoral and cellular immune responses induced by a fusion protein designed based on the truncated form of om ... | 2015 | 25723468 |
| context-dependent survival, fecundity and predicted population-level consequences of brucellosis in african buffalo. | chronic infections may have negative impacts on wildlife populations, yet their effects are difficult to detect in the absence of long-term population monitoring. brucella abortus, the bacteria responsible for bovine brucellosis, causes chronic infections and abortions in wild and domestic ungulates, but its impact on population dynamics is not well understood. we report infection patterns and fitness correlates of bovine brucellosis in african buffalo based on (1) 7 years of cross-sectional dis ... | 2015 | 25714466 |
| a new cis-encoded srna, bsrh, regulating the expression of hemh gene in brucella abortus 2308. | a total of 129 srna candidates were identified in brucella abortus 2308 in our previous work, and one candidate with potential to regulate expression of hemh gene was further analyzed in this study. we found that the novel srna can inhibit the expression of hemh and called it bsrh (brucella srna regulating hemh). the expression level of bsrh was tested in four different stress conditions. a significant upregulation was detected during the growth in acidic and brucella minimal media, as well as i ... | 2015 | 25670700 |
| failure of total hip arthroplasty secondary to infection caused by brucella abortus and the risk of transmission to operative staff. | infection of total knee or hip arthroplasty by brucella species is a rare complication. we describe the case of a failed hip replacement secondary to infection by brucella abortus, as well as presentation, treatment course, and 2-year follow-up. in addition, we review the literature for features of periprosthetic brucella species infections, and we describe the common exposures, clinical presentations, preoperative evaluation, and treatments used in the reported cases. furthermore, we discuss th ... | 2015 | 25658081 |
| the role of trem-2 in internalization and intracellular survival of brucella abortus in murine macrophages. | triggering receptor expressed on myeloid cells-2 (trem-2) is a cell surface receptor primarily expressed on macrophages and dendritic cells. trem-2 functions as a phagocytic receptor for bacteria as well as an inhibitor of toll like receptors (tlr) induced inflammatory cytokines. however, the role of trem-2 in brucella intracellular growth remains unknown. to investigate whether trem-2 is involved in brucella intracellular survival, we chose bone marrow derived macrophages (bmdms), in which trem ... | 2015 | 25563793 |
| evaluation of different primers for detection of brucella in human and animal serum samples by using pcr method. | the polymerase chain reaction (pcr) method can overcome the limitations of conventional methodology. the aim of this study is to evaluate three primer pairs broadly used b4/b5, f4/r2 and jpf/jpr, for detection of brucella by pcr, in human and animal serum samples and to determine analytic sensitivity of primers. | 2015 | 25556386 |
| survival of brucella abortus aqpx mutant in fresh and ripened cheeses. | the objective of this work was to evaluate the survival of a brucella abortus aqpx mutant during the elaboration and conservation of fresh and ripened cheeses at 4 °c and 24 °c. the ph values and water activity were monitored for each type of cheese. the fresh cheese was elaborated with raw milk inoculated with 6×10⁸ colony-forming units (cfu)/ml each of parental and mutant strain. ripening cheeses were elaborated with both raw and pasteurized milk and inoculated with 12×10⁸ cfu/ml each of paren ... | 2015 | 25551422 |
| mutation of purd and purf genes further attenuates brucella abortus strain rb51. | in the present study, transposon mutagenesis was used to further attenuate brucella abortus rb51 vaccine strain. two purd and purf mutants were constructed, characterized and evaluated for attenuation via intracellular survival in murine macrophage-like raw264.7 and hela cells, and by clearance in balb/c mice. the purd and purf mutants showed significantly decreased intracellular survival, and complementation of these mutants with intact copies of purd or purf genes of rb51 strain was able to re ... | 2015 | 25546140 |
| brucella abortus s19 and rb51 vaccine immunogenicity test: evaluation of three mice (balb/c, swiss and cd-1) and two challenge strains (544 and 2308). | the aim of the present study was to evaluate the use of different mouse strains (balb/c, swiss and cd-1) and different challenge strains (brucella abortus 544 and 2308) in the study of b. abortus vaccine (s19 and rb51) immunogenicity test in the murine model. no significant difference in b. abortus vaccine potency assay was found with the use of b. abortus 544 or b. abortus 2308 as challenge strain. results of variance analysis showed an interaction between treatment and mouse strain; therefore ... | 2015 | 25498211 |
| evaluation of the sensitivity and specificity of an enzyme-linked immunosorbent assay for diagnosing brucellosis in african buffalo (syncerus caffer). | brucellosis is a disease of veterinary and public health importance worldwide. in sub-saharan africa, where the bacterium brucella abortus has been identified in several free-ranging wildlife species, successful disease control may be dependent on accurate detection in wildlife reservoirs, including african buffalo (syncerus caffer). we estimated the sensitivity and specificity of a commercial enzyme-linked immunosorbent assay (elisa) (idexx brucellosis serum ab test, idexx laboratories, westbro ... | 2015 | 25397998 |
| vaccination of adult animals with a reduced dose of brucella abortus s19 vaccine to control brucellosis on dairy farms in endemic areas of india. | bovine brucellosis is an economically important disease which seriously affects dairy farming by causing colossal losses. it can be controlled by practicing vaccination of animals with brucella abortus s19 vaccine (s19 vaccine). in the present study, adult bovines were vaccinated on seven dairy farms with a reduced dose of s19 vaccine to control brucellosis. serological screening of adult animals (n = 1,082) by rose bengal test (rbt) and elisa prior to vaccination revealed the presence and absen ... | 2015 | 25274621 |
| plga (85:15) nanoparticle based delivery of rl7/l12 ribosomal protein in mice protects against brucella abortus 544 infection: a promising alternate to traditional adjuvants. | there is a compelling need for the development of suitable adjuvants for human use to enhance the efficacy of the upcoming vaccines for the prevention of life threatening infections. in the current study, we have tried to explore the immunogenic potential of nanoparticles (nps) made of plga (poly lactic-co-glycolic acid), a biodegradable and biocompatible polymer approved by fda for human use after entrapping rl7/l12 protein, an immunodominant antigen of brucella. adjuvant properties were exhibi ... | 2015 | 26442664 |
| functional analysis of bovine nramp1 and production of transgenic cloned embryos in vitro. | natural resistance-associated macrophage protein 1 (nramp1) plays an important role in restraining the growth of intracellular pathogens within macrophages. in this study, nramp1 cdna was cloned from qinchuan cattle and its anti-bacterial activity was demonstrated as being able to significantly inhibit the growth of salmonella abortusovis and brucella abortus in macrophages. calf fibroblasts stably transfected with psp-nramp1-ha vector were used to reconstruct bovine embryos by somatic cell nucl ... | 2015 | 23683995 |
| variables associated with infections of cattle by brucella abortus., leptospira spp. and neospora spp. in amazon region in brazil. | the frequency of neospora spp., leptospira spp. and brucella abortus infections in adult cattle was determined in herds of the state of pará, brazil, which is an important region for cattle production located in the amazon region. a total of 3466 adult female cattle from 176 herds were tested, leading to a frequency of seropositive animals of 14.7%, 3.7% and 65.5% and a herd positivity of 87.4%, 41.3% and 98.8% for infections caused by neospora spp., b. abortus and leptospira spp., respectively. ... | 2015 | 26302373 |
| ¹³c, ¹⁵n and ¹h backbone and side chain chemical shift assignment of acid-stress bacterial chaperone hdea at ph 6. | hdea is a small chaperone found in the periplasm of several common pathogenic bacteria (escherichia coli, shigella flexneri and brucella abortus) which are the leading causes of dysentery worldwide, especially in developing countries. its job is to protect other periplasmic proteins from aggregating as the bacteria pass through the low ph environment of the human stomach on their way to infect the intestines. hdea is an inactive folded dimer at neutral ph, but becomes a disordered active monomer ... | 2014 | 23835624 |
| in vivo-induced argininosuccinate lyase plays a role in the replication of brucella abortus in raw264.7 cells. | brucellosis caused by brucella species is a zoonotic disease with a serious impact on public health and the livestock industry. to better understand the pathogenesis of the disease, in vivo-induced antigen technology (iviat) was used to investigate the in vivo-induced antigens of brucella abortus in this study. a genomic expression library of b. abortus was constructed and screened using pooled bovine b. abortus-positive sera by iviat. in total, 33 antigens were identified. five antigens were fu ... | 2014 | 24421405 |
| human brucellosis in northwest ecuador: typifying brucella spp., seroprevalence, and associated risk factors. | human brucellosis in ecuador is underreported and based only on passive surveillance. since 2008, brucellosis was removed from the list of communicable diseases in the country. until now, the true human brucellosis picture has not yet been determined. the aim of this study was to determine the seroprevalence of the disease, identify risk factors associated with brucellosis seropositivity in humans, and isolate circulating strains of brucella spp. in the northwestern part of ecuador. between 2006 ... | 2014 | 24410144 |
| characterization of betaine aldehyde dehydrogenase (betb) as an essential virulence factor of brucella abortus. | the pathogenic mechanisms of brucellosis used to adapt to the harsh intracellular environment of the host cell are not fully understood. the present study investigated the in vitro and in vivo characteristics of b. abortus betaine aldehyde dehydrogenase (betb) (gene bank id: 006932) using a betb deletion mutant constructed from virulent b. abortus 544. in test under stress conditions, including osmotic- and acid stress-resistance, the betb mutant had a lower osmotic-resistance than b. abortus wi ... | 2014 | 24210811 |
| infection of c57bl/6 mice by trypanosoma musculi modulates host immune responses during brucella abortus cocolonization. | brucellosis, which results in fetal abortions in domestic and wildlife animal populations, is of major concern in the us and throughout much of the world. the disease, caused by brucella abortus, poses an economic threat to agriculture-based communities. a moderately efficacious live attenuated vaccine (b. abortus strain rb51) exists. however, even with vaccine use, outbreaks occur. evidence suggests that elk (cervus canadensis), a wild host reservoir, are the source of recent outbreaks in domes ... | 2014 | 24171573 |
| [detection of a clonal complex with brucella abortus biovar 2 genotype as founder in b. abortus isolates from argentina]. | brucella abortus is the causative agent of bovine brucellosis, a worldwide zoonosis. up to date, eight biovars of b. abortus have been described. in argentina, biovar 1 is the most frequently isolated. however, biovar 2, which is more pathogenic than biovar 1, is also found. molecular methods for subtyping isolates are necessary for allowing epidemiological surveillance and control of eradication programs. due to the genetic homogeneity of the genus brucella, the development of molecular typing ... | 2014 | 24401776 |
| a mouse model of anemia of inflammation: complex pathogenesis with partial dependence on hepcidin. | anemia is a common complication of infections and inflammatory diseases, but the few mouse models of this condition are not well characterized. we analyzed in detail the pathogenesis of anemia induced by an injection of heat-killed brucella abortus and examined the contribution of hepcidin by comparing wild-type (wt) to iron-depleted hepcidin-1 knockout (hamp-ko) mice. b abortus-treated wt mice developed severe anemia with a hemoglobin nadir at 14 days and partial recovery by 28 days. after an e ... | 2014 | 24357728 |
| isolation and identification of bovine brucella isolates from pakistan by biochemical tests and pcr. | brucellosis is endemic in bovines in pakistan. the brucella species and biovars involved, however, are unknown. the objectives of the present study were to isolate and characterize brucellae from seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes which had recently aborted. the seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes were collected from the potohar plateau, pakistan. isolation of brucellae was done on modified farre ... | 2014 | 23868548 |
| [relations between experimental infection with brucella abortus and avitaminosis b1 and c]. | 2014 | 24537704 | |
| bovine brucellosis in argentina and bordering countries: update. | bovine brucellosis is a zoonotic disease spread worldwide. the infection in cattle is predominantly caused by brucella abortus and is usually detected in pregnant females through abortions. the disease is endemic in argentina; however, infection in humans is underestimated and often not reported. the prevalence of bovine brucellosis in countries bordering argentina is quite variable: 0.04% in uruguay, 10.20% in the north and 0.06% in the south of brazil, 0.2% in chile, 3.15% in paraguay and 2.27 ... | 2014 | 23046031 |
| a potent brucella abortus 2308 δery live vaccine allows for the differentiation between natural and vaccinated infection. | brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. however, the current brucella abortus vaccines (s19 and rb51) are deficient; they can cause abortion in pregnant animals. moreover, when the vaccine s19 is used, tests cannot differentiate natural from vaccinated infection. therefore, a safer and more potent vaccine is needed. a brucella abortus 2308 ery promoter mutant (δery) was constructed to overcome these drawbacks. the growth of the δery mutant wa ... | 2014 | 24994009 |
| advancement of knowledge of brucella over the past 50 years. | fifty years ago, bacteria in the genus brucella were known to cause infertility and reproductive losses. at that time, the genus was considered to contain only 3 species: brucella abortus, brucella melitensis, and brucella suis. since the early 1960s, at least 7 new species have been identified as belonging to the brucella genus (brucella canis, brucella ceti, brucella inopinata, brucella microti, brucella neotomae, brucella ovis, and brucella pinnipedialis) with several additional new species u ... | 2014 | 24981716 |
| effects of partial deletion of the wzm and wzt genes on lipopolysaccharide synthesis and virulence of brucella abortus s19. | brucellosis is a worldwide human and animal infectious disease, and the effective methods of its control are immunisation of animals by vaccination and elimination. brucella abortus s19 is one of the popular vaccines with virulence in the control of cattle brucellosis. in the present study, allelic exchange plasmids of wzm and wzt genes and partial knockout mutants of wzm and wzt were constructed to evaluate the resulting difference in virulence of b. abortus s19. pcr analysis revealed that the ... | 2014 | 24718931 |
| proteomic analyses of the time course responses of mice infected with brucella abortus 544 reveal immunogenic antigens. | brucellosis is a major zoonotic disease caused by pathogens of the genus brucella. the eradication of brucellosis in domestic animals, associated with the prevention of human infection, can be attained through accurate diagnosis. however, the conventional serological diagnosis of brucellosis has limitations, particularly in detecting the infection period. accordingly, the aim of this study was to determine reliable immunogenic proteins to detect brucella abortus infection according to time cours ... | 2014 | 24975114 |
| evaluation of immunogenicity and protective efficacy of a plasmid dna vaccine encoding ribosomal protein l9 of brucella abortus in balb/c mice. | brucellosis is a worldwide zoonotic disease. no brucella vaccine is available for use in humans and existing animal vaccines have limitations. we have previously described the ribosomal protein l9 to have the vaccine potential. in this study, l9 based dna vaccine (pvaxl9) was generated and evaluated in mouse model. intramuscular immunisation of pvaxl9 was able to elicit the anti-l9 igg antibody response of both igg1 and igg2a isotypes when compared with pbs and pvax immunised control animals. he ... | 2014 | 24950353 |
| induction of immune responses by two recombinant proteins of brucella abortus, outer membrane proteins 2b porin and cu/zn superoxide dismutase, in mouse model. | the diagnosis of brucella abortus is mainly based on serological methods using antibody against lps, which has diagnostic problems. therefore, to solve this problem, we evaluated two proteins of b. abortus, cu/zn superoxide dismutase (sodc) and outer membrane proteins 2b porin (omp2b). the genes were cloned and expressed in a pmal system, and the recombinant proteins, romp2b and rsodc, were purified as fusion forms with maltosebinding protein. the identity of the proteins was confirmed by sds-pa ... | 2014 | 24608566 |
| novel influenza virus vectors expressing brucella l7/l12 or omp16 proteins in cattle induced a strong t-cell immune response, as well as high protectiveness against b. abortus infection. | this paper presents the results of a study of the immunogenicity and protectiveness of new candidate vector vaccine against brucella abortus - a bivalent vaccine formulation consisting of a mixture of recombinant influenza a subtype h5n1 or h1n1 (viral constructs vaccine formulation) viruses expressing brucella ribosomal protein l7/l12 and omp16, in cattle. to increase the effectiveness of the candidate vaccine, adjuvants such as montanide gel01 or chitosan were included in its composition. immu ... | 2014 | 24598723 |