Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| microbial diversity of the supra- and subgingival biofilm of healthy individuals after brushing with chlorhexidine- or silver-coated toothbrush bristles. | nanoparticulate silver has recently been reported as an effective antimicrobial agent. the aim of this clinical study was to investigate the potential changes on the oral microbiota of healthy individuals after controlled brushing with chlorhexidine- or silver-coated toothbrush bristles. twenty-four healthy participants were enrolled in this investigation and randomly submitted to 3 interventions. all the participants received, in a crossover format, the following toothbrushing interventions: (i ... | 2015 | 25581046 |
| active invasion of porphyromonas gingivalis and infection-induced complement activation in apoe-/- mice brains. | periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of alzheimer's disease. apoe-/- mice were orally infected (n = 12) with porphyromonas gingivalis, treponema denticola, tannerella forsythia, and fusobacterium nucleatum as mono- and polymicrobial infections. apoe-/- mice were sacrificed following 12 and 24 weeks of chronic infection. bacterial gen ... | 2015 | 25061055 |
| oral cavity anaerobic pathogens in biofilm formation on voice prostheses. | a polymerase chain reaction (pcr)-based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. the purpose of the present study was to determine the location of those pathogens inside the biofilms. | 2015 | 24677258 |
| quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: a case-control study. | a number of species/phylotypes have been newly implicated as putative periopathogens. the objective of this study was to explore associations among classical and new pathogens in subgingival biofilm and to assess their relative importance to chronic periodontitis. | 2015 | 25040261 |
| oral microbiome in chinese patients with aggressive periodontitis and their family members. | to investigate the microbiome composition in chinese patients with aggressive periodontitis (agp), and to compare the similarity of bacterial profiles between agp patients and their family members. | 2015 | 26412568 |
| periodontal status and pathogenic bacteria after gastric bypass: a cohort study. | the aim this study was to evaluate the influence of gastric bypass surgery (gbs) on periodontal disease and quantify the periodontopathogenic bacteria in patients undergoing this surgery. | 2015 | 25924695 |
| β2-glycoprotein i-dependent anti-cardiolipin antibodies associated with periodontitis in patients with systemic lupus erythematosus. | it was reported that patients with systemic lupus erythematosus (sle) exhibited increased levels of anticardiolipin (anti-cl) antibodies, a class of antiphospholipid antibodies associated with thrombosis. β2-glycoprotein i (β2gpi) has been considered as the actual target antigen for anti-cl antibodies. this study investigates the association of periodontal infection with anti-cl antibodies in patients with sle. | 2015 | 25817824 |
| occurrence of socransky red complex in pregnant women with and without periodontal disease. | to verify the presence of socransky red complex (porphyromonas gingivalis, treponema denticola and tannerella forsythia) and p. intermedia using polymerase chain reaction (pcr) in periodontally healthy pregnant women and pregnant women with periodontal disease, as well as its relation to arterial blood pressure and capillary glycaemia. | 2015 | 25386627 |
| the effect of conventional mechanical periodontal treatment on red complex microorganisms and clinical parameters in down syndrome periodontitis patients: a pilot study. | periodontal disease (pd) is induced by a complex microbiota, such as porphyromonas gingivalis, tannerella forsythia, and treponema denticola (together called the red complex), which triggers intense inflammatory reaction. down syndrome (ds) individuals demonstrate a high prevalence of pd compared with those who are otherwise chromosomally normal (euploids). this pilot study aimed to evaluate the effect of non-surgical periodontal treatment in ds chronic periodontitis patients on clinical and mic ... | 2015 | 25367612 |
| screening and development of dna aptamers specific to several oral pathogens. | aptamers are composed of single-stranded oilgonucleotides that can selectively bind desired molecules. it has been reported that rna or dna could act as not only a genetic messenger but also a catalyst in metabolic pathways. rna aptamers (average sizes 40-50 bp) are smaller than antibodies and have strong binding capacities to target molecules, similar to antigen-antibody interactions. once an aptamer was selected, it can be readily produced in large quantities at low cost. the objectives of thi ... | 2015 | 25269812 |
| pilot study on the clinical and microbiological effect of subgingival glycine powder air polishing using a cannula-like jet. | to assess the efficacy of subgingival glycine powder air polishing (gpap) during supportive periodontal therapy (spt). | 2015 | 25156125 |
| bioinformatic investigation of the cost management strategies of five oral microbes. | some amino acids are more energetically costly to synthesize de novo, therefore many microbes have evolved to regulate the metabolic expenditure of the cell and reduce the energy burden of extracellular unrecyclable proteins. several oral bacterial species take up amino acids and peptides obtained from proteolysis of host proteins and hence do not rely only on de novo synthesis. the aim of this study was to investigate if five oral bacterial species implement cost management strategies to reduce ... | 2015 | 25052707 |
| electrophoresis of periodontal pathogens in poly(ethyleneoxide) solutions with uncoated capillary. | periodontitis is a prevalent inflammatory disease caused by different species of anaerobic bacteria such as porphyromonas gingivalis (p.g), treponema denticola (t.d), and tannerella forsythia (t.f). we compared the separation result of dna ladders in hydroxyethyl cellulose, poly(ethyleneoxide) (peo), and polyethylene glycol and analyzed the effect of polymer concentration, electric field, and temperature of the background electrolyte on the separation performance. results demonstrated that there ... | 2015 | 25447490 |
| periimplant bacteria associated with different transmucosal designs or smoking habits. | this study is to compare periimplant microbiota associated with implant transmucosal designs or smoking habits. | 2015 | 25706261 |
| [clinical and putative periodontal pathogens' features of different sites with probing depth reduction after non-surgical periodontal treatment of patients with aggressive periodontitis]. | to evaluate the differences of clinical parameters and putative periodontal pathogens in sites of different probing depth (pd) reduction after non-surgical periodontal treatment in patients with aggressive periodontitis (agp). | 2015 | 25686322 |
| association between the presence of treponema denticola and reduced levels of antiatherogenic high density lipoprotein in periodontitis. | dyslipidemia, a disorder in the levels of cholesterol or lipoproteins in the plasma, is a feature of atherosclerosis, leading to cardiovascular disease. the objective of this study was to evaluate whether tanerella forsythia and treponema denticola are related to reduced levels of antiatherogenic high density lipoprotein (hdl). | 2015 | 25328922 |
| [the molecular genetic characteristic of species content of saliva and gingival recess under periodontitis]. | the examination was carried out of samplings of 110 patients with periodontitis (observation group) and 60 patients without pathology of periodont (comparison group). the polymerase chain reaction was used to analyze samples of saliva and contents of periodontal recesses for detecting species-specific dna fragments of porphymmonas gigngivalis, streptococcus macacae, s. mutans, s. oralis, s. salivarius, s. sangis, s. sobrinus, treponema denticola. in patients with periodontitis s. mutans, s. oral ... | 2015 | 27032255 |
| functional biomarkers for chronic periodontitis and insights into the roles of prevotella nigrescens and fusobacterium nucleatum; a metatranscriptome analysis. | periodontitis is the most prevalent inflammatory disease worldwide and is caused by a dysbiotic subgingival biofilm. here we used metatranscriptomics to determine the functional shift from health to periodontitis, the response of individual species to dysbiosis and to discover biomarkers. | 2015 | 28721234 |
| presence of digital dermatitis treponemes on cattle and sheep hoof trimming equipment. | digital dermatitis (dd) is an infectious foot disease causing severe lameness in dairy cattle (worldwide) and sheep (uk). this study investigated whether dd treponema phylogroups can be present on equipment used to trim ruminant hooves and, therefore, consider this trimming equipment as a possible vector for the transmission of dd. equipment was tested after being used to trim dd symptomatic and asymptomatic cattle and sheep hooves, and subsequently after disinfection of equipment. after trimmin ... | 2014 | 24821857 |
| mechanisms of il-8 suppression by treponema denticola in gingival epithelial cells. | the purpose of this study was to investigate the mechanism(s) of interleukin (il)-8 suppression by treponema denticola, one of the major periodontal pathogens, in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with wild-type (wt), dentilisin-deficient (k1) or flagellin-deficient (flge) t. denticola in the presence or absence of 2% human serum for 24 h. the levels of il-8 expression were measured with real-time reverse transcription pcr and elisa. in ... | 2014 | 24296811 |
| subgingival microbial profile and production of proinflammatory cytokines in chronic periodontitis. | this review examines literature data concerning the bacterial findings in chronic periodontitis depending on pocket depth, and presents the latest published information on the presence of proinflammatory factors in periodontal environment. it has been found that chronic periodontitis affects as much as 80% of the middle-aged population; by comparison, the prevalence of aggressive periodontitis reaches up to 1-1.5%. it is accepted that this social disease is multifactorial in etiology, but the ev ... | 2014 | 25434071 |
| isolation of treponema and tannerella spp. from equine odontoclastic tooth resorption and hypercementosis related periodontal disease. | red complex bacteria, i.e. porphyromonas gingivalis, treponema denticola and tannerella forsythia, are involved in the onset and progression of periodontal disease in man, yet seldom inhabit the oral cavity of healthy individuals. periodontal disease is also encountered in horses, with equine odontoclastic tooth resorption and hypercementosis (eotrh) constituting a particular form of disease. however, only little is known about the oral microbiome of healthy and periodontitis-affected equids. | 2014 | 23742079 |
| oral inflammation, tooth loss, risk factors, and association with progression of alzheimer's disease. | periodontitis is a polymicrobial chronic inflammatory disease of tooth-supporting tissues with bacterial etiology affecting all age groups, becoming chronic in a subgroup of older individuals. periodontal pathogens porphyromonas gingivalis, tannerella forsythia, and treponema denticola are implicated in the development of a number of inflammatory pathologies at remote organ sites, including alzheimer's disease (ad). the initial inflammatory hypothesis proposed that ad hallmark proteins were the ... | 2014 | 24946875 |
| molecular analysis of oral bacteria in dental biofilm and atherosclerotic plaques of patients with vascular disease. | oral bacteria have been detected in atherosclerotic plaques at a variable frequency; however, the connection between oral health and vascular and oral bacterial profiles of patients with vascular disease is not clearly established. the aim of this study was to evaluate the presence of oral bacterial dna in the mouth and atherosclerotic plaques, in addition to assessing the patients' caries and periodontal disease history. | 2014 | 24820755 |
| periodontal disease associated with red complex bacteria in dogs. | red complex bacteria (treponema denticola, tannerella forsythia and porphyromonas gingivalis) play a major role in the aetiology of periodontal disease in humans. this study was designed to evaluate the association of such bacteria with periodontal disease in dogs. | 2014 | 24450418 |
| oral hygiene reinforcement in the simplified periodontal treatment of 1 hour. | to compare the clinical and microbiological outcome of the 1-h ultrasonic debridement of chronic periodontitis patients (cpp) with and without frequent sessions of oral hygiene reinforcement. | 2014 | 24255993 |
| dentilisin involvement in coaggregation between treponema denticola and tannerella forsythia. | periodontitis arises from a biofilm consisting of gram-negative anaerobic rods and spirochetes. porphyromonas gingivalis, treponema denticola, and tannerella forsythia, termed the red complex, have been co-isolated with high frequency from chronic periodontitis lesions, and these microorganisms are thought to be major pathogens of the disease. coaggregation is an important strategy in the colonization of dental plaque biofilm by these bacteria. in the present study, we investigated the coaggrega ... | 2014 | 25152229 |
| quantification of periodontal pathogens cell counts by capillary electrophoresis. | gingivitis is a highly prevalent periodontal disease around the worldwide. porphyromonas gingivalis (p.g), treponema denticola (t.d) and tannerela forsythia (t.f) were considered to be three important periodontal pathogens related to gingivitis, and research shows that the counts of periodontal pathogen cells in the patients before, during, and after fixed orthodontic appliance therapy were quite different. we proposed a simple method to extract the periodontal pathogens from the periodontal poc ... | 2014 | 25145561 |
| effects of ginkgo biloba extract on periodontal pathogens and its clinical efficacy as adjuvant treatment. | to determine the effects of ginkgo biloba extract (egb) on major periodontal pathogens in subgingival plaque. | 2014 | 25098260 |
| [comparison of the effects of ginkgo biloba extract and minocycline hydrochlovide on periodontitis]. | to compare the clinical effect of ginkgo biloba extract gel (ginkgo biloba extract,egb) and minocycline hydrochloride (periocline) on periodontitis and their inhibition on putative periodontal pathogens. | 2014 | 25199931 |
| periodontal bacteria in human carotid atherothrombosis as a potential trigger for neutrophil activation. | epidemiological, biological and clinical links between periodontal and cardiovascular diseases are now well established. several human studies have detected bacterial dna corresponding to periodontal pathogens in cardiovascular samples. intraplaque hemorrhage has been associated with a higher risk of atherosclerotic plaque rupture, potentially mediated by neutrophil activation. in this study, we hypothesized that plaque composition may be related to periodontal pathogens. | 2014 | 25173070 |
| subgingival microbial profile and production of proinflammatory cytokines in chronic periodontitis. | this review examines literature data concerning the bacterial findings in chronic periodontitis depending on pocket depth, and presents the latest published information on the presence of proinflammatory factors in periodontal environment. it has been found that chronic periodontitis affects as much as 80% of the middle-aged population; by comparison, the prevalence of aggressive periodontitis reaches up to 1-1.5%. it is accepted that this social disease is multifactorial in etiology, but the ev ... | 2014 | 25507669 |
| molecular detection of bacteria associated to dental caries in 4-12-year-old tunisian children. | the occurrence of several microbial species in the oral cavity of 4-12-year-old tunisian children was investigated. samples were taken from 158 children (81 caries actives and 77 caries free). genomic dna was extracted and analyzed for the presence of 17 microbial species using a polymerase chain reaction assay. all samples were positive for at least one of the target microbial strains. streptococcus mutans was the most prevalent species (76.5%) detected in genomic dna collected from carious les ... | 2014 | 24814824 |
| targeting cystalysin, a virulence factor of treponema denticola-supported periodontitis. | cystalysin from treponema denticola is a pyridoxal 5'-phosphate dependent lyase that catalyzes the formation of pyruvate, ammonia, and sulfide from cysteine. it is a virulence factor in adult periodontitis because its reaction contributes to hemolysis, which sustains the pathogen. therefore, it was proposed as a potential antimicrobial target. to identify specific inhibitors by structure-based in silico methods, we first validated the crystal structure of cystalysin as a reliable starting point ... | 2014 | 24616267 |
| what role do periodontal pathogens play in osteoarthritis and periprosthetic joint infections of the knee? | through the use of polymerase chain reaction (pcr)-electron spray ionization (esi)-time of flight (tof)-mass spectrometry (ms), we identified multiple periodontal pathogens within joint tissues of individuals undergoing replacement arthroplasties of the knee. the most prevalent of the periodontal pathogens were treponema denticola and enterococcus faecalis, the latter of which is commonly associated with apical periodontitis. these findings were unique to periprosthetic joint infections (pji) of ... | 2014 | 24921460 |
| inter-bacterial correlations in subgingival biofilms: a large-scale survey. | although the complexity of the oral ecology and the ecological differences between health and disease are well accepted, a clear view on the dynamics in relation to disease is lacking. in this study, the prevalence and abundance of 20 key oral bacteria was assessed in health and disease and more importantly a closer look was given to the inter-bacterial relationships. | 2014 | 24102517 |
| molecular identification and antibiotic resistant bacteria isolated from primary dentition infections. | bacterial resistance to antibiotics is a health problem in many parts of the world. the aim of this study was to identify bacteria from dental infections and determine bacterial resistance to antibiotics used in dental care in the primary dentition. | 2014 | 25091293 |
| soluble triggering receptor expressed on myeloid cells 1 (strem-1) in gingival crevicular fluid: association with clinical and microbiologic parameters. | soluble triggering receptor expressed on myeloid cells 1 (strem-1) belongs to the immunoglobulin superfamily and is involved in amplification of the inflammatory response to bacterial infection. this cross-sectional study aims to investigate the levels of strem-1 in gingival crevicular fluid (gcf) of individuals without periodontitis and with chronic periodontitis (cp) or generalized aggressive periodontitis (gagp) and their association with the levels of key periodontal pathogens in subgingival ... | 2014 | 23659423 |
| detection of eight periodontal microorganisms and distribution of porphyromonas gingivalis fima genotypes in chinese patients with aggressive periodontitis. | the microbiologic feature of aggressive periodontitis (agp) in chinese patients has not yet been determined. this study aims to investigate the prevalence of eight periodontal microorganisms and the distribution of the porphyromonas gingivalis fima genotype in a cohort of chinese patients with agp. | 2014 | 23646850 |
| genetic analysis of porphyromonas gingivalis (fima), aggregatibacter actinomycetemcomitans, and red complex in coronary plaque. | the objective of the present study was to detect the presence of porphyromonas gingivalis (fima), aggregatibacter actinomycetemcomitans, and red complex in the coronary plaque of patients with coronary artery disease. | 2014 | 23447375 |
| cluster of bacteria associated with peri-implantitis. | information on the microbiota in peri-implantitis is limited. we hypothesized that neither gender nor a history of periodontitis/smoking or the microbiota at implants differ by implant status. | 2014 | 23527870 |
| periodontal pathogens and associated factors in aggressive periodontitis: results 5-17 years after active periodontal therapy. | to assess the association between presence of periodontal pathogens and recurrence of disease in patients with aggressive periodontitis (agp) after active periodontal therapy (apt) and further influencing factors. | 2014 | 24708362 |
| biology and pathogenesis of cytomegalovirus in periodontal disease. | human periodontitis is associated with a wide range of bacteria and viruses and with complex innate and adaptive immune responses. porphyromonas gingivalis, tannerella forsythia, aggregatibacter actinomycetemcomitans, treponema denticola, cytomegalovirus and other herpesviruses are major suspected pathogens of periodontitis, and a combined herpesvirus-bacterial periodontal infection can potentially explain major clinical features of the disease. cytomegalovirus infects periodontal macrophages an ... | 2014 | 24320955 |
| molecular microbiological evaluation of subgingival biofilm sampling by paper point and curette. | the present clinical study aimed to investigate if there are differences in microbiological outcomes dependent on the subgingival biofilm collection method. subgingival biofilm samples were collected from the four deepest pockets (>5 mm) of 17 patients with aggressive periodontitis (agp) and 33 patients with chronic periodontitis (cp), first by paper point and thereafter by curette. samples obtained with the same method were pooled together from each patient and forwarded for molecular microbiol ... | 2014 | 23879704 |
| serum antibody responses to periodontal microbiota in chronic and aggressive periodontitis: a postulate revisited. | the authors revisited the 1999 international workshop postulate of robust serum antibody responses to infecting agents in localized aggressive periodontitis (lagp) and weak responses in generalized aggressive periodontitis (gagp). antibody responses were further examined in localized and generalized chronic periodontitis (lcp and gcp). | 2014 | 23725029 |
| detection and quantification of periodontal pathogens in smokers and never-smokers with chronic periodontitis by real-time polymerase chain reaction. | the purpose of the present investigation is to compare the presence and number of periodontal pathogens in the subgingival microbiota of smokers versus never-smokers with chronic periodontitis and matched probing depths (pds) using real-time polymerase chain reaction (rt-pcr). | 2014 | 24794687 |
| genetic characteristics and pathogenic mechanisms of periodontal pathogens. | periodontal disease is caused by a group of bacteria that utilize a variety of strategies and molecular mechanisms to evade or overcome host defenses. recent research has uncovered new evidence illuminating interesting aspects of the virulence of these bacteria and their genomic variability. this paper summarizes some of the strategies utilized by the major species - aggregatibacter actinomycetemcomitans, tannerella forsythia, treponema denticola, and porphyromonas gingivalis - implicated in the ... | 2014 | 24736700 |
| rare case of generalised aggressive periodontitis in the primary dentition. | generalised aggressive periodontitis (ap) in the prepubescent age is an exceptionally rare disease in the primary dentition of otherwise healthy children. characteristics of ap are gingival inflammation, deep periodontal pockets, bone loss, tooth mobility and even tooth loss. the most common way of treating this disease is the extraction of all the involved primary teeth. | 2014 | 25001360 |
| prevalence of six periodontal pathogens in subgingival samples of italian patients with chronic periodontitis. | the aim of this study was to investigate the subgingival prevalence of six periodontal pathogens in 352 italian patients with chronic periodontitis. possible correlations with clinical parameters, age, gender and smoking status were also investigated. at first visit a pooled subgingival plaque sample was obtained for each subject by using the paper-point method. the samples were processed and analysed according to a commercially available quantitative real time polymerase chain reaction assay (m ... | 2014 | 25387289 |
| effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis. | short chain fatty acids (scfas) play important roles in periodontal diseases. however, the concentrations of scfas in gingival crevicular fluid of patients with aggressive periodontitis are not known. the aim of this intervention study was to investigate the influences of non-surgical periodontal therapy on levels of scfas in the gingival crevicular fluid of patients with generalized aggressive periodontitis (g-agp), and analyze the concentrations of scfas in sites with or without the detected p ... | 2014 | 25340203 |
| microbiological effects of periodontal therapy plus azithromycin in patients with diabetes: results from a randomized clinical trial. | current evidence suggests that periodontal infection may aggravate diabetes control. the aim of this study was to determine the changes in the frequency with which porphyromonas gingivalis, tannerella forsythia, treponema denticola and aggregatibacter actinomycetemcomitans were detected in patients with diabetes with the use of non-surgical therapy plus azithromycin in a randomized clinical trial. one hundred and five (105) patients with diabetes and chronic periodontitis were randomly assigned ... | 2014 | 25523961 |
| detection of periodontal pathogens in the patients with aortic aneurysm. | the occurrence and development of aortic aneurysm (aa) are associated with infection. some researchers have detected the dna of periodontal pathogens in aa samples in certain populations. however, it has not been done in chinese population. the objective of this study was to evaluate the prevalence of periodontal pathogens in oral tissue samples and aneurysm samples of aa patients. | 2014 | 25430459 |
| microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. | the detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. the collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. however, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. the aim of this cross-sectional s ... | 2014 | 24144271 |
| colonisation of gingival epithelia by subgingival biofilms in vitro: role of "red complex" bacteria. | biofilm formation on tooth surface results in colonisation and invasion of the juxtaposed gingival tissue, eliciting strong inflammatory responses that lead to periodontal disease. this in vitro study investigated the colonisation of human gingival multi-layered epithelium by multi-species subgingival biofilms, and evaluated the relative effects of the "red complex" species (porphyromonas gingivalis, tannerella forsythia and treponema denticola). | 2014 | 24949828 |
| 16s rdna-based metagenomic analysis of dental plaque and lung bacteria in patients with severe acute exacerbations of chronic obstructive pulmonary disease. | acute exacerbations of chronic obstructive pulmonary disease (ae-copd) are leading causes of mortality in hospital intensive care units. we sought to determine whether dental plaque biofilms might harbor pathogenic bacteria that can eventually cause lung infections in patients with severe ae-copd. | 2014 | 24484531 |
| the association between detectable plasmatic human immunodeficiency virus (hiv) viral load and different subgingival microorganisms in brazilian adults with hiv: a multilevel analysis. | this study investigates the association between detectable plasmatic human immunodeficiency virus (hiv) viral load (hvl) and high levels of periodontal- and non-periodontal-related microorganisms in the subgingival microbiota of individuals with hiv. | 2014 | 23952074 |
| sensitive real-time pcr detection of pathogenic leptospira spp. and a comparison of nucleic acid amplification methods for the diagnosis of leptospirosis. | bacteria of the genus leptospira, the causative agents of leptospirosis, are categorized into pathogenic and non-pathogenic species. however, the benefit of using a clinical diagnostic that is specific for pathogenic species remains unclear. in this study, we present the development of a real-time pcr (rtpcr) for the detection of pathogenic leptospira (the pathogenic rtpcr), and we perform a comparison of the pathogenic rtpcr with a published assay that detects all leptospira species [the undiff ... | 2014 | 25379890 |
| collection and characterization of samples for establishment of a serum repository for lyme disease diagnostic test development and evaluation. | serological assays and a two-tiered test algorithm are recommended for laboratory confirmation of lyme disease. in the united states, the sensitivity of two-tiered testing using commercially available serology-based assays is dependent on the stage of infection and ranges from 30% in the early localized disease stage to near 100% in late-stage disease. other variables, including subjectivity in reading western blots, compliance with two-tiered recommendations, use of different first- and second- ... | 2014 | 25122862 |
| metagenomic analysis reveals presence of treponema denticola in a tissue biopsy of the iceman. | ancient hominoid genome studies can be regarded by definition as metagenomic analyses since they represent a mixture of both hominoid and microbial sequences in an environment. here, we report the molecular detection of the oral spirochete treponema denticola in ancient human tissue biopsies of the iceman, a 5,300-year-old copper age natural ice mummy. initially, the metagenomic data of the iceman's genomic survey was screened for bacterial ribosomal rna (rrna) specific reads. through ranking th ... | 2014 | 24941044 |
| initial characterization of the flge hook high molecular weight complex of borrelia burgdorferi. | the spirochete periplasmic flagellum has many unique attributes. one unusual characteristic is the flagellar hook. this structure serves as a universal joint coupling rotation of the membrane-bound motor to the flagellar filament. the hook is comprised of about 120 flge monomers, and in most bacteria these structures readily dissociate to monomers (∼ 50 kda) when treated with heat and detergent. however, in spirochetes the flge monomers form a large mass of over 250 kda [referred to as a high mo ... | 2014 | 24859001 |
| superoxide dismutases and superoxide reductases. | 2014 | 24684599 | |
| membrane lipids in agrobacterium tumefaciens: biosynthetic pathways and importance for pathogenesis. | many cellular processes critically depend on the membrane composition. in this review, we focus on the biosynthesis and physiological roles of membrane lipids in the plant pathogen agrobacterium tumefaciens. the major components of a. tumefaciens membranes are the phospholipids (pls), phosphatidylethanolamine (pe), phosphatidylglycerol, phosphatidylcholine (pc) and cardiolipin, and ornithine lipids (ols). under phosphate-limited conditions, the membrane composition shifts to phosphate-free lipid ... | 2014 | 24723930 |
| analysis of the complement sensitivity of oral treponemes and the potential influence of fh binding, fh cleavage and dentilisin activity on the pathogenesis of periodontal disease. | treponema denticola, a periopathogen, evades complement-mediated killing by binding the negative complement regulatory protein factor h (fh) to its surface via the fhbb protein. paradoxically, bound fh is cleaved by t. denticola's dentilisin protease, a process hypothesized to trigger localized dysregulation of complement activation in periodontal pockets. the ability of other oral treponemes to evade complement-mediated killing and bind and cleave fh has not been assessed. in this report, we de ... | 2014 | 24815960 |
| comparative genome analysis and identification of competitive and cooperative interactions in a polymicrobial disease. | polymicrobial diseases are caused by combinations of multiple bacteria, which can lead to not only mild but also life-threatening illnesses. periodontitis represents a polymicrobial disease; porphyromonas gingivalis, treponema denticola and tannerella forsythia, called 'the red complex', have been recognized as the causative agents of periodontitis. although molecular interactions among the three species could be responsible for progression of periodontitis, the relevant genetic mechanisms are u ... | 2014 | 25171331 |
| comparative genome analysis and identification of competitive and cooperative interactions in a polymicrobial disease. | polymicrobial diseases are caused by combinations of multiple bacteria, which can lead to not only mild but also life-threatening illnesses. periodontitis represents a polymicrobial disease; porphyromonas gingivalis, treponema denticola and tannerella forsythia, called 'the red complex', have been recognized as the causative agents of periodontitis. although molecular interactions among the three species could be responsible for progression of periodontitis, the relevant genetic mechanisms are u ... | 2014 | 25171331 |
| prevalence of antibiotic resistance genes in subjects with successful and failing dental implants. a pilot study. | to investigate the prevalence of the bacterial genes encoding resistance to beta-lactams, tetracyclines and metronidazole respectively, in subjects with successful and failing dental implants and to assess the presence of staphylococcus aureus and the meca gene encoding for methicillin resistant staphylococcus aureus (mrsa) in the same samples. | 2014 | 25646133 |
| prevalence of antibiotic resistance genes in subjects with successful and failing dental implants. a pilot study. | to investigate the prevalence of the bacterial genes encoding resistance to beta-lactams, tetracyclines and metronidazole respectively, in subjects with successful and failing dental implants and to assess the presence of staphylococcus aureus and the meca gene encoding for methicillin resistant staphylococcus aureus (mrsa) in the same samples. | 2014 | 25646133 |
| effects of adjunctive daily phototherapy on chronic periodontitis: a randomized single-blind controlled trial. | the purpose of this randomized single-blind controlled trial was to elucidate the clinical and antimicrobial effects of daily phototherapy (pt) as an adjunct to scaling and root planing (srp) in patients with chronic periodontitis. | 2014 | 25568808 |
| association between immunoglobulin g1 against tannerella forsythia and reduction in the loss of attachment tissue. | to evaluate whether the levels of immunoglobulin g (igg) antibody to tanerella forsythia are associated with periodontal status. | 2014 | 25568807 |
| n-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice. | we previously reported that human serum significantly reduces the invasion of various oral bacterial species into gingival epithelial cells in vitro. the aims of the present study were to characterize the serum component(s) responsible for the inhibition of bacterial invasion of epithelial cells and to examine their effect on periodontitis induced in mice. | 2014 | 25568806 |
| porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type ix secretion system and gliding motility. | porphyromonas gingivalis is a gram-negative, non-motile, anaerobic bacterium implicated as a major pathogen in periodontal disease. p. gingivalis grows as black-pigmented colonies on blood agar, and many bacteriologists have shown interest in this property. studies of colonial pigmentation have revealed a number of important findings, including an association with the highly active extracellular and surface proteinases called gingipains that are found in p. gingivalis. the por secretion system, ... | 2014 | 25546073 |
| porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type ix secretion system and gliding motility. | porphyromonas gingivalis is a gram-negative, non-motile, anaerobic bacterium implicated as a major pathogen in periodontal disease. p. gingivalis grows as black-pigmented colonies on blood agar, and many bacteriologists have shown interest in this property. studies of colonial pigmentation have revealed a number of important findings, including an association with the highly active extracellular and surface proteinases called gingipains that are found in p. gingivalis. the por secretion system, ... | 2014 | 25546073 |
| complete genome sequence for treponema sp. omz 838 (atcc 700772, dsm 16789), isolated from a necrotizing ulcerative gingivitis lesion. | the oral treponeme bacterium treponema sp. omz 838 was originally isolated from a human necrotizing ulcerative gingivitis (nug) lesion. its taxonomic status remains uncertain. the complete genome sequence length was determined to be 2,708,067 bp, with a g+c content of 44.58%, and 2,236 predicted coding dna sequences (cds). | 2014 | 25540346 |
| prevalence and quantitative analysis of epstein-barr virus dna and porphyromonas gingivalis associated with japanese chronic periodontitis patients. | a number of studies have recently suggested epstein-barr virus (ebv) involvement in the pathogenesis of periodontitis. in this study, we investigated the association between major periodontopathic bacteria porphyromonas gingivalis (p. gingivalis) and ebv in japanese chronic periodontitis (cp) patients. | 2014 | 25515271 |
| prevalence and quantitative analysis of epstein-barr virus dna and porphyromonas gingivalis associated with japanese chronic periodontitis patients. | a number of studies have recently suggested epstein-barr virus (ebv) involvement in the pathogenesis of periodontitis. in this study, we investigated the association between major periodontopathic bacteria porphyromonas gingivalis (p. gingivalis) and ebv in japanese chronic periodontitis (cp) patients. | 2014 | 25515271 |
| fimbriae-mediated outer membrane vesicle production and invasion of porphyromonas gingivalis. | porphyromonas gingivalis is a keystone periopathogen that plays an essential role in the progress of periodontitis. like other gram-negative bacteria, the ability of p. gingivalis to produce outer membrane vesicles is a strategy used to interact with, and survive within its biological niches. here we compared the protein components associated with vesicles derived from a fimbriated strain (33277) and an afimbriated strain (w83) of p. gingivalis using proteomic analyses. some well-known virulence ... | 2014 | 25524808 |
| fimbriae-mediated outer membrane vesicle production and invasion of porphyromonas gingivalis. | porphyromonas gingivalis is a keystone periopathogen that plays an essential role in the progress of periodontitis. like other gram-negative bacteria, the ability of p. gingivalis to produce outer membrane vesicles is a strategy used to interact with, and survive within its biological niches. here we compared the protein components associated with vesicles derived from a fimbriated strain (33277) and an afimbriated strain (w83) of p. gingivalis using proteomic analyses. some well-known virulence ... | 2014 | 25524808 |
| serum igg antibody levels to periodontal microbiota are associated with incident alzheimer disease. | periodontitis and alzheimer disease (ad) are associated with systemic inflammation. this research studied serum igg to periodontal microbiota as possible predictors of incident ad. | 2014 | 25522313 |
| identification of dipeptidyl-peptidase (dpp)5 and dpp7 in porphyromonas endodontalis, distinct from those in porphyromonas gingivalis. | dipeptidyl peptidases (dpps) that liberate dipeptides from the n-terminal end of oligopeptides are crucial for the growth of porphyromonas species, anaerobic asaccharolytic gram negative rods that utilize amino acids as energy sources. porphyromonas endodontalis is a causative agent of periapical lesions with acute symptoms and asp/glu-specific dpp11 has been solely characterized in this organism. in this study, we identified and characterized two p. endodontalis dpps, dpp5 and dpp7. cell-associ ... | 2014 | 25494328 |
| subgingival microbial profiles of sudanese patients with aggressive periodontitis. | aggressive periodontitis (agp) is prevalent and shows a rapid course in african individuals. although a strong focus has been placed on aggregatibacter actinomycetemcomitans, new methods support the existence of a complex subgingival microflora in agp. the purpose of the present study was to map the subgingival microbiota as well as explore the presence of a. actinomycetemcomitans and the jp2 clone in a group of sudanese individuals with agp, using different analytical methods. | 2014 | 25487558 |
| subgingival microbial profiles of sudanese patients with aggressive periodontitis. | aggressive periodontitis (agp) is prevalent and shows a rapid course in african individuals. although a strong focus has been placed on aggregatibacter actinomycetemcomitans, new methods support the existence of a complex subgingival microflora in agp. the purpose of the present study was to map the subgingival microbiota as well as explore the presence of a. actinomycetemcomitans and the jp2 clone in a group of sudanese individuals with agp, using different analytical methods. | 2014 | 25487558 |
| a 17-year old patient with dock8 deficiency, severe oral hsv-1 and aggressive periodontitis - a case of virally induced periodontitis? | we present a 17-year old girl with dock-8 deficiency, severe untreated oral hsv-1 infection and associated aggressive periodontitis. dock-8 deficiency is a primary immunodeficiency, caused by biallelicloss-of-function mutations in the dock8 gene, often leading to severe viral and fungal mucocutaneous infections. nevertheless, to date dock8 has not been associated with severe periodontitis and inflammatory bone loss around teeth. understanding whether dock8 deficiency or severe hsv-1 infection un ... | 2014 | 25600604 |
| a 17-year old patient with dock8 deficiency, severe oral hsv-1 and aggressive periodontitis - a case of virally induced periodontitis? | we present a 17-year old girl with dock-8 deficiency, severe untreated oral hsv-1 infection and associated aggressive periodontitis. dock-8 deficiency is a primary immunodeficiency, caused by biallelicloss-of-function mutations in the dock8 gene, often leading to severe viral and fungal mucocutaneous infections. nevertheless, to date dock8 has not been associated with severe periodontitis and inflammatory bone loss around teeth. understanding whether dock8 deficiency or severe hsv-1 infection un ... | 2014 | 25600604 |
| s-nitrosoglutathione accelerates recovery from 5-fluorouracil-induced oral mucositis. | mucositis induced by anti-neoplastic drugs is an important, dose-limiting and costly side-effect of cancer therapy. | 2014 | 25478918 |
| rho and rnase play a central role in fmn riboswitch regulation in corynebacterium glutamicum. | riboswitches are rna elements that regulate gene expression in response to their ligand. although these regulations are thought to be performed without any aid of other factors, recent studies suggested the participation of protein factors such as transcriptional termination factor rho and rnase in some riboswitch regulations. however, to what extent these protein factors contribute to the regulation was unclear. here, we studied the regulatory mechanism of the flavin mononucleotide (fmn) ribosw ... | 2014 | 25477389 |
| rho and rnase play a central role in fmn riboswitch regulation in corynebacterium glutamicum. | riboswitches are rna elements that regulate gene expression in response to their ligand. although these regulations are thought to be performed without any aid of other factors, recent studies suggested the participation of protein factors such as transcriptional termination factor rho and rnase in some riboswitch regulations. however, to what extent these protein factors contribute to the regulation was unclear. here, we studied the regulatory mechanism of the flavin mononucleotide (fmn) ribosw ... | 2014 | 25477389 |
| interleukin-6 c.-174g>c polymorphism and periodontitis in a brazilian population. | aim. periodontitis is an inflammatory disease that affects the teeth supporting structures, triggered by periodontal pathogens, and is influenced by environmental and genetic factors. genes encoding molecules related to the immune response, such as cytokine, are the main candidates for polymorphisms analysis and may be possibly associated with this pathology. a g/c promoter polymorphism on the il6 gene has been shown to affect basal il-6 levels. the aim of this study was to investigate the assoc ... | 2014 | 25548674 |
| diversity-generating retroelements in phage and bacterial genomes. | diversity-generating retroelements (dgrs) are dna diversification machines found in diverse bacterial and bacteriophage genomes that accelerate the evolution of ligand-receptor interactions. diversification results from a unidirectional transfer of sequence information from an invariant template repeat (tr) to a variable repeat (vr) located in a protein-encoding gene. information transfer is coupled to site-specific mutagenesis in a process called mutagenic homing, which occurs through an rna in ... | 2014 | 26104433 |
| next-generation models of human cardiogenesis via genome editing. | cardiogenesis is one of the earliest and most important steps during human development and is orchestrated by discrete families of heart progenitors, which build distinct regions of the fetal heart. for the past decade, a lineage map for the distinct subsets of progenitors that generate the embryonic mammalian heart has begun to lay a foundation for the development of new strategies for rebuilding the adult heart after injury, an unmet clinical need for the vast majority of patients with end-sta ... | 2014 | 25237142 |
| strand invasion based amplification (siba®): a novel isothermal dna amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte. | isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (pcr) in that they do not require thermal cycling or sophisticated laboratory equipment. however, non-target-dependent amplification has limited the sensitivity of isothermal technologies and complex probes are usually required to distinguish between non-specific and target-dependent amplification. here, we report a novel isothermal nucleic acid amplification technology, strand invasion ... | 2014 | 25419812 |
| polymicrobial synergy and dysbiosis in inflammatory disease. | uncontrolled inflammation of the periodontal area may arise when complex microbial communities transition from a commensal to a pathogenic entity. communication among constituent species leads to polymicrobial synergy between metabolically compatible organisms that acquire functional specialization within the developing community. keystone pathogens, even at low abundance, elevate community virulence, and the resulting dysbiotic community targets specific aspects of host immunity to further disa ... | 2014 | 25498392 |
| polymicrobial synergy and dysbiosis in inflammatory disease. | uncontrolled inflammation of the periodontal area may arise when complex microbial communities transition from a commensal to a pathogenic entity. communication among constituent species leads to polymicrobial synergy between metabolically compatible organisms that acquire functional specialization within the developing community. keystone pathogens, even at low abundance, elevate community virulence, and the resulting dysbiotic community targets specific aspects of host immunity to further disa ... | 2014 | 25498392 |
| shifts in campylobacter species abundance may reflect general microbial community shifts in periodontitis progression. | oral campylobacter species have been found to be associated with periodontitis progression. while the etiological significance of campylobacter rectus is quite established, the association of c. gracilis, c. concisus, and c. curvus with health or disease remains contradictory. | 2014 | 25412608 |
| oral bacterial dna findings in pericardial fluid. | we recently reported that large amounts of oral bacterial dna can be found in thrombus aspirates of myocardial infarction patients. some case reports describe bacterial findings in pericardial fluid, mostly done with conventional culturing and a few with pcr; in purulent pericarditis, nevertheless, bacterial pcr has not been used as a diagnostic method before. | 2014 | 25412607 |
| the novel species streptococcus tigurinus and its association with oral infection. | streptococcus tigurinus is a novel species of viridans streptococci, shown to cause severe invasive infections such as infective endocarditis, spondylodiscitis and meningitis. s. tigurinus belongs to the streptococcus mitis group and is most closely related to streptococcus mitis, streptococcus oralis, streptococcus pneumoniae, streptococcus pseudopneumoniae and streptococcus infantis. the presence of s. tigurinus in the human oral cavity has been documented, including in patients with periodont ... | 2014 | 25483862 |
| the novel species streptococcus tigurinus and its association with oral infection. | streptococcus tigurinus is a novel species of viridans streptococci, shown to cause severe invasive infections such as infective endocarditis, spondylodiscitis and meningitis. s. tigurinus belongs to the streptococcus mitis group and is most closely related to streptococcus mitis, streptococcus oralis, streptococcus pneumoniae, streptococcus pseudopneumoniae and streptococcus infantis. the presence of s. tigurinus in the human oral cavity has been documented, including in patients with periodont ... | 2014 | 25483862 |
| host antimicrobial peptides in bacterial homeostasis and pathogenesis of disease. | innate immune responses function as a first line of host defense against the development of bacterial infection, and in some cases to preserve the sterility of privileged sites in the human host. bacteria that enter these sites must counter host responses for colonization. from the host's perspective, the innate immune system works expeditiously to minimize the bacterial threat before colonization and subsequent dysbiosis. the multifactorial nature of disease further challenges predictions of ho ... | 2014 | 26029470 |
| characterization of treponema denticola mutants defective in the major antigenic proteins, msp and tmpc. | treponema denticola, a gram-negative and anaerobic spirochete, is associated with advancing severity of chronic periodontitis. in this study, we confirmed that two major antigenic proteins were msp and tmpc, and examined their physiological and pathological roles using gene-deletion mutants. msp formed a large complex that localized to the outer membrane, while tmpc existed as a monomer and largely localized to the inner membrane. however, tmpc was also detected in the outer membrane fraction, b ... | 2014 | 25401769 |
| pre-steady-state kinetic and structural analysis of interaction of methionine γ-lyase from citrobacter freundii with inhibitors. | methionine γ-lyase (mgl) catalyzes the γ-elimination of l-methionine and its derivatives as well as the β-elimination of l-cysteine and its analogs. these reactions yield α-keto acids and thiols. the mechanism of chemical conversion of amino acids includes numerous reaction intermediates. the detailed analysis of mgl interaction with glycine, l-alanine, l-norvaline, and l-cycloserine was performed by pre-steady-state stopped-flow kinetics. the structure of side chains of the amino acids is impor ... | 2014 | 25398880 |
| pre-steady-state kinetic and structural analysis of interaction of methionine γ-lyase from citrobacter freundii with inhibitors. | methionine γ-lyase (mgl) catalyzes the γ-elimination of l-methionine and its derivatives as well as the β-elimination of l-cysteine and its analogs. these reactions yield α-keto acids and thiols. the mechanism of chemical conversion of amino acids includes numerous reaction intermediates. the detailed analysis of mgl interaction with glycine, l-alanine, l-norvaline, and l-cycloserine was performed by pre-steady-state stopped-flow kinetics. the structure of side chains of the amino acids is impor ... | 2014 | 25398880 |