Publications

purification and characterization of recombinant forms of murine tcl1 proteins.	the tcl1 gene, which is located on chromosome 14, plays a major role in human hematopoietic malignancies and encodes a 14-kda protein whose function has not been determined. this gene is expressed in pre-b cells, in immature thymocytes, and, at low levels, in activated t cells but not in peripheral mature b cells and in normal cells. the tcl1 protein is similar in its primary structure to a protein encoded by the mature t-cell proliferation gene (mtcp1). the mtcp1 gene is located on the x chromo ...	2000	10733880
partial liquid ventilation with perflubron attenuates in vivo oxidative damage to proteins and lipids.	to determine the impact of partial liquid ventilation on the degree of pulmonary damage by reactive oxygen species in a model of acute lung injury caused by systemic endotoxemia.	2000	10667523
cloning, sequencing, and expression of the tulip bulb chitinase-1 cdna.	a cdna encoding tulip bulb chitinase-1 (tbc-1) was cloned using a combination of immunoscreening from a lambda zap cdna library with anti-tbc-1 antiserum and the 5' rapid amplification of cdna end (race) method, and sequenced. the cdna consists of 1,106 nucleotides and included an open reading frame encoding a polypeptide of 314 amino acids. comparison of the deduced amino acid sequence and the determined protein sequence indicated the presence of a signal peptide and an extra peptide composed o ...	2000	10945255
a flexible single-step detection of blotted antigen using a fusion protein between protein a and green fluorescent protein.	a green fluorescent protein mutant (s147p gfp) was fused with protein a and expressed in escherichia coli. this fusion protein (pa-gfp147) was used in immunoblotting studies as a new detection system, designated as "flexible single-step detection (fssd)". in fssd, the detection of blotted antigen was done in one step, and the antigen-antibody reaction can be monitored by uv-irradiation in real time. the reaction time, therefore, can be flexibly controlled by monitoring the green fluorescence.	2000	10945281
a small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heat-denatured protein.	small heat shock proteins (shsps) are a diverse group of heat-induced proteins that are conserved in prokaryotes and eukaryotes and are especially abundant in plants. recent in vitro data indicate that shsps act as molecular chaperones to prevent thermal aggregation of proteins by binding non-native intermediates, which can then be refolded in an atp-dependent fashion by other chaperones. we used heat-denatured firefly luciferase (luc) bound to pea (pisum sativum) hsp18.1 as a model to define th ...	2000	10631262
selenoprotein w accumulates primarily in primate skeletal muscle, heart, brain and tongue.	the human selenoprotein w coding region with the selenocysteine codon (tga) changed to a cysteine codon (tgt) was fused to six histidine codons (at its 3' end), cloned into a prokaryotic expression vector (ptrc99a), and the corresponding mutated selenoprotein w was expressed in bacteria. the protein was purified by ni-nta agarose column and reverse phase hplc. polyclonal antibodies raised against this protein were used in western blots to determine tissue distribution of selenoprotein w from rhe ...	2000	10718624
segmented filamentous bacteria prevent colonization of enteropathogenic escherichia coli o103 in rabbits.	despite their distribution in the intestines of many mammals, including man, segmented filamentous bacteria (sfb) have not been found in rabbits, nor has any function been identified for these uncultivable microbes. new zealand white rabbits were infected with rabbit enteropathogenic escherichia coli o103 (repec o103) derivatives, followed up clinically, and randomly killed 1-4 days after inoculation. intestinal tissue samples were examined by electron and light microscopy to search for sfb and ...	2000	10720527
the n-terminal region of the heme-regulated eif2alpha kinase is an autonomous heme binding domain.	the n-terminal domain (ntd) of the heme-regulated eukaryotic initiation factor (eif)2alpha kinase (hri) was aligned to sequences in the ncbi data base using entrez and a pam250 matrix. significant similarity was found between amino acids 11-118 in the ntd of rabbit hri and amino acids 16-120 in mammalian alpha-globins. several conserved amino acid residues present in globins are conserved in the ntd of hri. his83 of hri was predicted to be equivalent to the proximal heme ligand (hisf8) that is c ...	2000	10632719
a comparative study of human muscle and brain creatine kinases expressed in escherichia coli.	we report the expression of the human muscle (ck-mm) and brain (ck-bb) creatine kinases in escherichia coli. the proteins have been purified to apparent homogeneity and several of their physical and kinetic properties investigated. in the process, we have conclusively verified the correct dna sequence of the genes encoding the respective isozymes, and determined the correct primary structure and mass of the gene products. alignment of the primary sequences of these two enzymes shows 81% sequence ...	2000	10882173
engineering microsomal cytochrome p450 2c5 to be a soluble, monomeric enzyme. mutations that alter aggregation, phospholipid dependence of catalysis, and membrane binding.	deletion of the n-terminal membrane-spanning domain from microsomal p450s 2c5 and 2c3 generates the enzymes, 2c5dh and 2c3dh, that exhibit a salt-dependent association with membranes indicating that they retain a monofacial membrane interaction domain. the two proteins are tetramers and dimers, respectively, in high salt buffers, and only 2c5dh requires phospholipids to reconstitute fully the catalytic activity of the enzyme. amino acid residues derived from p450 2c3dh between residues 201 and 2 ...	2000	10644712
engineered salt-insensitive alpha-defensins with end-to-end circularized structures.	we designed a retro-isomer and seven circularized "beta-tile" peptide analogs of a typical rabbit alpha-defensin, np-1. the analogs retained defensin-like architecture after the characteristic end-to-end, cys(3,31) (c i:c vi), alpha-defensin disulfide bond was replaced by a backbone peptide bond. the retro-isomer of np-1 was as active as the parent compound, suggesting that overall topology and amphipathicity governed its antimicrobial activity. a beta-tile design with or without a single cross- ...	2000	10660548
molecular cloning and characterization of a unique 60 kda/72 kda antigen gene encoding enzyme i of the phosphoenolpyruvate: sugar phosphotransferase system (pts) of mycoplasma hyopneumoniae.	the recombinant clone expressing a 60 kda (p60) antigen was isolated from escherichia coli by screening a lambda embl3 genomic library using rabbit produced antiserum against mycoplasma hyopneumoniae. sequence analysis revealed that an interrupted (by a uga codon) open reading frame coding for a 72 kda protein (p72) may contain the p60 antigen gene. western blot analysis with an anti-p60 monospecific antibody confirmed the presence of a p72 antigen from the total protein of m. hyopneumoniae, and ...	2000	10920262
failure of female baboons (papio anubis) to conceive following immunization with recombinant non-human primate zona pellucida glycoprotein-b expressed in escherichia coli.	progress in the development of an immunocontraceptive vaccine based on zona pellucida glycoproteins has been hampered due to observed ovarian dysfunction associated with immunization using these as immunogens. in this study four female baboons (papio anubis) were immunized with recombinant bonnet monkey (macaca radiata) zona pellucida glycoprotein-b (r-bmzpb) expressed in escherichia coli and conjugated to diphtheria toxoid (dt) using arlacel-a and squalene as adjuvants. all the immunized animal ...	2000	10825598
development of eia for detection of chlamydia trachomatis in genital specimens.	a double antibody sandwich enzyme immunoassay (eia) for chlamydial antigen detection was developed using a monoclonal antibody against lipopolysaccharide (lps) of chlamydia trachomatis as a coating antibody. polyclonal rabbit antiserum against partially purified antigen from elementary body (eb) antibody and horse-radish peroxidase conjugated goat anti-rabbit antibody were used as the primary and secondary antibody respectively. the developed eia could detect protein of partially purified eb at ...	2000	11023074
intrauterine infection induces programmed cell death in rabbit periventricular white matter.	an association between chorioamnionitis and periventricular leukomalacia has been reported in human preterm infants. however, whether this link is causal has not been convincingly established, and the underlying molecular mechanisms remain unclear. the objective of this study was to establish a reproducible model of cerebral white matter disease in preterm rabbits after intrauterine infection. escherichia coli was inoculated into both uterine horns of laparotomized pregnant rabbits when gestatio ...	2000	10832730
the effect of single residue substitutions of serine-283 on the strength of head-to-tail interaction and actin binding properties of rabbit skeletal muscle alpha-tropomyosin.	vertebrate skeletal muscle alpha-tropomyosin polymerizes in a head-to-tail manner and binds cooperatively to actin. it has been postulated that the cooperative actin binding is governed by the strength of the head-to-tail interaction. in order to know the relationship between the head-to-tail affinity and actin binding, we studied the properties of tropomyosin variants with single residue substitutions at serine-283, the penultimate residue at the carboxyl terminus that is involved in the head-t ...	2000	10833280
functional and immunological analysis of recombinant mouse h- and l-ferritins from escherichia coli.	the production and characterization of recombinant mouse h- and l-ferritin chains from escherichia coli are described. the proteins were efficiently expressed and purified with yields of 7-40 mg per liter of cell culture. they had the expected molecular mass and showed a physical stability analogous to that of the corresponding human ferritins. mouse h- and l-ferritins had a very similar mobility on denaturing sds-page, but could be readily separated on nondenaturing page because of the distinct ...	2000	10833409
endothelin-1 impairs neutrophil respiratory burst and elimination of escherichia coli in rabbits.	during systemic inflammation, elevated levels of endothelin (et)-1 have been reported. the aim of this study was to investigate the effects of et-1 on neutrophil (pmn) respiratory burst, phagocytosis, and elimination of escherichia coli from blood and tissues.	2000	10834705
selecting and evolving functional proteins in vitro by ribosome display.		2000	11075357
effects of mci-154, a calcium sensitizer, on cardiac dysfunction in endotoxic shock in rabbits.	this study was designed to observe the effects of mci-154, a calcium sensitizer, on cardiac dysfunction after endotoxic shock in rabbits. ten hours after the rabbits were given injection of 1.0 mg/kg endotoxin (escherichia coli, o111:b4) via marginal ear veins, 0.1 mg/kg mci-154 was injected intravenously and then 50 ml/kg normal saline (ns) + 0.1 mg/kg mci-154 was infused continuously at a rate of 0.7 ml/min. during this process, the parameters of cardiac function were measured. it was found th ...	2000	10847633
human herpes virus 8 interleukin-6 homologue triggers gp130 on neuronal and hematopoietic cells.	human herpes virus-8 (hhv8) encodes a cytokine named viral interleukin-6 (vil-6) that shares 25% amino-acid identity with its human homologue. human il-6 is known to be a growth and differentiation factor of lymphatic cells and plays a potential role in the pathophysiology of various lymphoproliferative diseases. vil-6 is expressed in hhv8-associated-diseases including kaposi's sarcoma, body-cavity-based-lymphoma and castleman's disease, suggesting a pathogenetic involvement in the malignant gro ...	2000	10848977
role of tyrosine 65 in the mechanism of serine hydroxymethyltransferase.	crystal structures of human and rabbit cytosolic serine hydroxymethyltransferase have shown that tyr65 is likely to be a key residue in the mechanism of the enzyme. in the ternary complex of escherichia coli serine hydroxymethyltransferase with glycine and 5-formyltetrahydrofolate, the hydroxyl of tyr65 is one of four enzyme side chains within hydrogen-bonding distance of the carboxylate group of the substrate glycine. to probe the role of tyr65 it was changed by site-directed mutagenesis to phe ...	2000	10858298
preclinical and clinical evidence for disappearance of long-circulating characteristics of polyethylene glycol liposomes at low lipid dose.	this study describes the effect of the lipid dose of (99m)tc-polyethylene glycol (peg) liposomes in the low-dose range (0. 02-1.0 micromol/kg) on the pharmacokinetics and biodistribution in rats, rabbits, and humans. the biodistribution and pharmacokinetics of (99m)tc-peg liposomes at various dose levels were studied in rats and rabbits with a focal escherichia coli infection. scintigraphic images were recorded on a gamma camera. in addition, the role of macrophages in the biodistribution of a l ...	2000	10869403
a recombinant eimeria protein inducing interferon-gamma production: comparison of different gene expression systems and immunization strategies for vaccination against coccidiosis.	a rabbit antiserum against an 18- to 27-kd native protein fraction (f3) from eimeria acervulina merozoites identified a cdna (3-1e) containing a 1086-base pair insertion with an open reading frame of 170 amino acids (predicted molecular weight, 18,523). the recombinant 3-1e cdna expressed in escherichia coli produced a 60-kd fusion protein and a 23-kd protein after factor xa treatment of the fusion protein. both proteins were reactive with the f3 antiserum by western blot analysis. a rabbit anti ...	2000	10879919
detection and characterisation of the genes encoding glyoxalase i and ii from neisseria meningitidis.	glyoxalase enzymes i and ii are involved in a detoxification process consisting of conversion of reactive dicarbonyl compounds (e.g., methylglyoxal) to less reactive hydroxy acids. the structural gene for meningococcal glyoxalase i (gloa) was identified by screening an expression library with a rabbit antiserum. the meningococcal gloa gene consisted of 138 deduced amino acids, with a calculated mol. wt of 15.7 kda. the dna and deduced protein sequence of gloa was compared to known sequences of g ...	2000	10882093
genetic characterization of dna region containing the trh and ure genes of vibrio parahaemolyticus.	we have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. here, we cloned and sequenced the 15,754-bp dna region containing the trh gene and the gene cluster for urease production from the chromosome of clinical v. parahaemolyticus (th3996). we found 16 open reading frames (o ...	2000	10992480
aminoglycoside antibiotics mediate context-dependent suppression of termination codons in a mammalian translation system.	the translation machinery recognizes codons that enter the ribosomal a site with remarkable accuracy to ensure that polypeptide synthesis proceeds with a minimum of errors. when a termination codon enters the a site of a eukaryotic ribosome, it is recognized by the release factor erf1. it has been suggested that the recognition of translation termination signals in these organisms is not limited to a simple trinucleotide codon, but is instead recognized by an extended tetranucleotide termination ...	2000	10917599
adeno-associated virus type 2 nonstructural protein rep78 suppresses translation in vitro.	adeno-associated virus type 2 nonstructural protein rep78 [621 amino acids (aa) long] affects the expression of various cellular and viral genes. in this study we examined the effects of rep78 on expression of the luciferase gene from the human cytomegalovirus immediate-early promoter in hela cells and on translation of rna encoding luciferase in rabbit reticulocyte lysate. when rep78 and luciferase were coexpressed, the luciferase activity decreased despite increased levels of luciferase mrna i ...	2000	10612674
translocation of group 1 capsular polysaccharide to the surface of escherichia coli requires a multimeric complex in the outer membrane.	surface expression of the group 1 k30 capsular polysaccharide of escherichia coli strain e69 (o9a:k30) requires wza(k30), a member of the outer membrane auxiliary (oma) protein family. a mutation in wza(k30) severely restricts the formation of the k30 capsular structure on the cell surface, but does not interfere with the biosynthesis or polymerization of the k30 repeat unit. here we show that wza(k30) is a surface-exposed outer membrane lipoprotein. wza(k30) multimers form ring-like structures ...	2000	10619844
characterization of the african swine fever virus protein p49: a new late structural polypeptide.	the open reading frame b438l, located within the ecori b fragment of the african swine fever virus genome, is predicted to encode a protein of 438 amino acids with a molecular mass of 49.3 kda. it presents a cell attachment rgd (arg-gly-asp) motif but no other significant similarity to protein sequences in databases. northern blot and primer extension analysis showed that b438l is transcribed only at late times during virus infection. the b438l gene product has been expressed in escherichia coli ...	2000	10640542
pulsed ultrasound enhances the killing of escherichia coli biofilms by aminoglycoside antibiotics in vivo.	escherichia coli biofilms on two polyethylene disks were implanted subcutaneously into rabbits receiving systemic gentamicin. ultrasound was applied for 24 h to one disk. both disks were removed, and viable bacteria were counted. pulsed ultrasound significantly reduced bacterial viability below that of nontreated biofilms without damage to the skin.	2000	10681355
cell vacuolation, a manifestation of the el tor hemolysin of vibrio cholerae.	culture supernatants of nontoxigenic nonepidemic clinical strains of vibrio cholerae belonging to diverse serogroups were found to induce vacuolation of nonconfluent hela cells. the vacuoles became prominent 18 h after introduction of culture supernatant, and vacuolated cells survived for 48 h and then died. only a fraction of the vacuolated cells took up neutral red dye, implying that there were differences in the vacuolar microenvironment. further tests showed that the factor responsible for v ...	2000	10722584
role of tir and intimin in the virulence of rabbit enteropathogenic escherichia coli serotype o103:h2.	attaching and effacing (a/e) rabbit enteropathogenic escherichia coli (repec) strains belonging to serogroup o103 are an important cause of diarrhea in weaned rabbits. like human epec strains, they possess the locus of enterocyte effacement clustering the genes involved in the formation of the a/e lesions. in addition, pathogenic repec o103 strains produce an esp-dependent but eae (intimin)-independent alteration of the host cell cytoskeleton characterized by the formation of focal adhesion comp ...	2000	10722617
serine hydroxymethyltransferase and threonine aldolase: are they identical?	serine hydroxymethyltransferase, a pyridoxal phosphate-dependent enzyme, catalyses the interconversion of serine and glycine, both of which are major sources of one-carbon units necessary for the synthesis of purine, thymidylate, methionine, and so on. threonine aldolase catalyzes the pyridoxal phosphate-dependent, reversible reaction between threonine and acetaldehyde plus glycine. no extensive studies have been carried out on threonine aldolase in animal tissues, and it has long been believed ...	2000	10716626
identification and expression of an allergen asp f 13 from aspergillus fumigatus and epitope mapping using human ige antibodies and rabbit polyclonal antibodies.	the aspergillus genus of fungi is known to be one of the most prevalent aeroallergens. on two-dimensional immunoblotting using patients' sera containing ige specific for asp f 13, an allergen with a molecular mass of 33 kda and a pi of 6.2 was identified. this allergen was also present in a. fumigatus culture filtrates. furthermore, the sequence of the asp f 13 cdna was identical to that for alkaline protease isolated from a. fumigatus and showed 42-49% identity of amino acids with two proteases ...	2000	10677362
endotoxin-induced lung inflammation is independent of the complement membrane attack complex.	several products of the activated complement system are known to modulate endothelial cell function in vitro. it has been shown that the membrane attack complex (mac) (c5b-c9) can enhance tumor necrosis factor alpha (tnf-alpha)-induced expression of p- and e-selectin and intercellular adhesion molecule type 1 in cell cultures of human umbilical vein endothelial cells. in the present study the potential role of this synegism for lung injury during endotoxin-mediated septic shock in vivo was exami ...	2000	10678982
lipopolysaccharide entry in the damaged cornea and specific uptake by polymorphonuclear neutrophils.	bacterial lipopolysaccharide (lps) is an important agent of induction of ocular pathology following corneal injury or wearing of contaminated contact lenses. the mechanism of lps uptake through the corneal epithelium is unclear, and the role played by inflammatory cells in this phenomenon has not been previously assessed. fluorescein isothiocyanate-labeled lps from escherichia coli was deposited onto the abraded corneas of new zealand white rabbits. epifluorescence microscopy of living excised c ...	2000	10678999
expression of recombinant norwalk-like virus capsid proteins using a bacterial system and the development of its immunologic detection.	the capsid protein of norwalk-like virus (nlv) isolates nlv-36 (mexico virus type, genogroup ii [gii]), nlv-21 (lordsdale virus type, gii), nlv-114 (untyped gii virus), and nlv-96-908 (ky89 virus type, gi) have been expressed in an escherichia coli system. the expressed recombinant nlv capsid proteins, fused with maltose binding protein (mbp-rv) and thioredoxin (trx-rv) in e. coli lysate, were analyzed using sodium dodecyl sulfate-polyacrylamide gel eletrophoresis. rabbit igg (r-igg) in hyperimm ...	2000	10686033
infectious agents associated with rabbit pneumonia: isolation of amyxomatous myxoma virus strains.	sixty-six rabbits, with no history of vaccination against myxomatosis and which had died of pulmonary lesions, were submitted for virological and serological tests for myxoma virus (mv) infection and for bacteriological examinations. at post mortem, the diagnoses based on observed lesions were as follows: acute haemorrhagic pneumonia (38%); acute suppurative bronchopneumonia (35%); and fibrinohaemorrhagic bronchopneumonia with fibrinous pleuritis (27%). mv was isolated from 10% of the rabbits, m ...	2000	10712805
the bacillus subtilis cell division protein ftsl localizes to sites of septation and interacts with divic.	ftsl is a small bitopic membrane protein required for vegetative cell division and sporulation in bacillus subtilis. we investigated its localization by fluorescence microscopy using a green fluorescent protein (gfp) fusion. gfp-ftsl was localized at mid-cell in vegetative cells and at the asymmetric septum in sporulating cells. we also show that ftsl forms a ring-like structure at the division site and that it remains localized at mid-cell during the whole septation process. by yeast two-hybrid ...	2000	10844672
a 99tcm-labelled leukotriene b4 receptor antagonist for scintigraphic detection of infection in rabbits.	in a search for a rapid and accurate imaging agent for scintigraphic detection of infection and inflammation, an ltb4 receptor antagonist, 99tcm-rp517, which contains the hydrazino nicotinamide moiety, has been developed recently. to study the in vivo behaviour of 99tcm-rp517, rabbits with escherichia coli infection were injected intravenously with 99tcm-rp517. gamma camera images were obtained and ex vivo bio-distribution was determined at several hours post-injection (p.i.). in a separate set ...	2000	11192710
effect of natural antioxidants and apocynin on lps-induced endotoxemia in rabbit.	the objective of this study was to compare the prophylactic effects of the natural antioxidant from spinach (nao) and apocynin, on the hepatic oxidative stress and liver damage induced by lipopolysaccharide (lps). male new zealand rabbits were challenged with lps with or without 8 days of antioxidant pretreatment. pretreatment with nao, but not apocynin, significantly (p < 0.05) decreased the levels of hydroperoxides and malondialdehyde (mda) in the liver cytosolic fraction and the activity of n ...	2000	11211238
properties of recombinant htra: an otitis media vaccine candidate antigen from non-typeable haemophilus influenzae.	non-encapsulated or non-typable haemophilus influenzae (nthi) is a major cause of middle ear infections in young children. htra has been identified as a vaccine candidate antigen from nthi; therefore physicochemical characterization of this antigen is important for vaccine development. recombinant nthi htra has been expressed in e. coli and shown to have serine protease activity. several mutant, recombinant htra proteins were expressed and purified to obtain suitable vaccine antigens lacking pro ...	2000	11214237
an investigation into the pathogenic properties of escherichia coli strains blr, bl21, dh5alpha and eq1.	to examine escherichia coli strains eq1, dh5alpha, blr and bl21 for known pathogenic mechanisms.	2000	11123478
three-dimensional structure of rk-1: a novel alpha-defensin peptide.	nmr spectroscopy and simulated annealing calculations have been used to determine the three-dimensional structure of rk-1, an antimicrobial peptide from rabbit kidney recently discovered from homology screening based on the distinctive physicochemical properties of the corticostatins/defensins. rk-1 consists of 32 residues, including six cysteines arranged into three disulfide bonds. it exhibits antimicrobial activity against escherichia coli and activates ca(2+) channels in vitro. through its p ...	2000	11123900
the asp-rich region at the carboxyl-terminus of calsequestrin binds to ca(2+) and interacts with triadin.	calsequestrin (csq) is a high capacity ca(2+) binding protein in the junctional sarcoplasmic reticulum of striated muscles, and has been shown to regulate the ryanodine receptor (ryr) through triadin and junctin. in order to identify the functional roles of specific regions on csq, several csq deletion mutants were prepared by molecular cloning and escherichia coli expression. 45ca(2+) overlay assay using a native gel system revealed that the major ca(2+) binding motif of csq resides in the asp- ...	2000	11113462
a single endotoxin injection in the rabbit causes prolonged blood vessel dysfunction and a procoagulant state.	to determine the duration of vascular blood vessel dysfunction and coagulation abnormalities after administration of endotoxin in a nonlethal septic rabbit model.	2000	11098972
functional characterization of green fluorescent protein-profilin fusion proteins.	to clarify the role of profilins in cells, fusion proteins constructed with green fluorescent protein (gfp) should be extremely helpful. as profilins are considerably smaller than the gfp fusion partner (14-17 kda compared with 27 kda, respectively), we characterized the fusion proteins in vitro, to ascertain their biological function. we fused mouse profilin i and ii to either the c-terminus or n-terminus of gfp. these fusion proteins were expressed in escherichia coli and affinity-purified on ...	2000	10931210
effect of l-arginine on endothelial injury and hemostasis in rabbit endotoxin shock.	to investigate whether impaired endothelial function was related to alteration of nitric oxide (no) formation during endotoxic shock, we studied the effects of supplementation of l-arginine (l-arg), d-arginine (d-arg), and n(g)-nitro-l-arginine methyl ester (l-name), on endothelial function and structure in a rabbit model. endotoxic shock was induced by a single lipopolysaccharide bolus (0.5 mg/kg i.v., escherichia coli endotoxin). coagulation factors and expression of monocyte tissue factor wer ...	2000	11053330
contribution of defined amino acid residues to the immunogenicity of recombinant escherichia coli heat-stable enterotoxin fusion proteins.	we investigated whether the toxicity-associated receptor-binding domain of the non-immunogenic escherichia coli heat-stable enterotoxin (sth) as a fusion with a carrier protein and the inclusion of an appropriate spacer are critical factors for eliciting antibody responses against the native toxin. the immunological properties of three toxic and one non-toxic fusion proteins, consisting of sth n-terminally joined to the c-terminus of the major subunit clpg of e. coli cs31a fimbriae, were compare ...	2000	11064199
epithelial cell adherence mediated by the enterotoxigenic escherichia coli tia protein.	in vitro studies have shown that enterotoxigenic escherichia coli (etec) strains are capable of invading cultured epithelial cells derived from the human ileum and colon. two separate invasion loci (tia and tib) have previously been isolated from the classical etec strain h10407. the tia locus has been shown to direct the synthesis of tia, a 25-kda outer membrane protein. tia is sufficient to confer the adherence and invasion phenotypes on laboratory stains of e. coli, suggesting that this prote ...	2000	11083770
histologic inflammation in the maternal and fetal compartments in a rabbit model of acute intra-amniotic infection.	this study was undertaken to determine the course of acute inflammation in the maternal and fetal compartments during experimentally induced ascending intra-amniotic infection.	2000	11084546
tracing the origin of the ractk1 k(+) channel.	potassium secretion by the kidney is vital for the maintenance of k(+) homeostasis. ractk1, a putative inwardly rectifying potassium channel cloned from cultured rabbit collecting duct cells, has been proposed to play a role in this process. however, the lack of homology with any other cloned potassium channel and the inability to reproduce the results across different laboratories has brought into question the existence of ractk1. recently, it has been suggested that ractk1 is a contamination f ...	2000	11027655
functional recombinant rabbit muscle phosphoglucomutase from escherichia coli.	the gene coding for phosphoglucomutase (pgm) from oryctolagus cuniculus (rabbit) has been expressed in escherichia coli under a t7 expression system with a his-tag. about half of the expressed pgm protein was present in inclusion bodies, but this protein was inactive when solubilized. the protein in the soluble cell fraction was isolated and purified in one step on a ni-nta column. the eluate from this column was adjusted to 95% saturated ammonium sulfate, and the resulting protein precipitate w ...	2000	11035960
impairment of cardiac beta-adrenoceptor cellular signaling by decreased expression of g(s alpha) in septic rabbits.	abnormalities in the beta-adrenergic control of cardiac function play a role in the pathogenesis of several disease states. because circulatory failure in patients with septic shock is known to be less responsive to catecholamines, we investigated whether the beta-adrenoceptor-linked signal transduction mechanisms are altered in the heart of a septic animal model	2000	11149442
protection against leptospirosis by immunization with plasmid dna encoding 33 kda endoflagellin of l. interrogans serovar lai.	to evaluate how the efficacy of dna inocutation affects the ability to raise protective immunity against leptospira.	2000	12899392
immune response to escherichia coli antigens entrapped in liposomes. i. immunopathological studies.	in this study, we have searched for an effective mucosal delivery system for a purified e. coli antigen which elicits anticolonization and anti-toxic immunity. e. coli colonization factor antigen (cfa/i) and heat-labile enterotoxin (lt) were encapsulated in liposomes. to determine the efficacies of soluble and liposome-encapsulated e. coli antigens young rabbits were mucosally treated with three oral doses of e. coli antigens given 7 days apart. ten days after the last booster, rabbits were oral ...	1999	11845460
defense mechanisms of urinary bladder: studies on antimicrobial polypeptides from bladder mucosa.	the acid-soluble extract of the bladder mucosal surface was obtained by washing out the bladder with dilute acetic acid in the presence of protease inhibitors. the wash-out materials from rats, rabbits, pigs, and humans manifested strong bactericidal activity against e. coli in vitro. the ultrafiltrate of the human material, which contained two major peptides with apparent molecular masses of 6.7 kd and 8.5 kd, respectively, showed potent bactericidal activity against e. coli, pseudomonas aerugi ...	1999	12899379
the risk of nosocomial pneumonia is not increased during partial liquid ventilation.	to determine whether partial liquid ventilation (plv) affects the risk of nosocomial pneumonia.	1999	10628620
macrophage tnf mrna expression induced by lps is regulated by sphingomyelin metabolites.	metabolism of macrophage (mo) membrane phospholipids produces key mediators of inflammation and major second messengers that modulate inflammatory responses during sepsis. sphingomyelin is a major class of phospholipid that releases ceramide and sphingosine. this study was designed to investigate the involvement of sphingomyelin metabolites in mo activation by lipopolysaccharide (lps). rabbit alveolar mo were obtained by bronchoalveolar lavage and exposed to c6-ceramide, a cell-permeable analogu ...	1999	10454830
the identification of a second cofilin binding site on actin suggests a novel, intercalated arrangement of f-actin binding.	the cofilins are members of a protein family that binds monomeric and filamentous actin, severs actin filaments, and increases monomer off-rate from the pointed end. here, we characterize the cofilin-actin interface. we confirm earlier work suggesting the importance of the lower region of subdomain 1 encompassing the n and c termini (site 1) in cofilin binding. in addition, we report the discovery of a new cofilin binding site (site 2) from residues 112-125 that form a helix toward the upper, re ...	1999	10506133
a reverse-sandwich enzyme-linked immunosorbent assay for verocytotoxin 1 and 2 antibodies in human and bovine sera.	a reverse-sandwich enzyme-linked immunosorbent assay (elisa), in which an antibody is sandwiched by antigens, was established for the titration of antibodies to verocytotoxins (vt) in human and animal sera. this assay has two advantages over a conventional indirect elisa: (i) higher specificity and sensitivity and (ii) the ability to comparably titrate antibodies from different species. the vt1 (shiga-like toxin 1) antibody-positive rates were 5% in 202 normal adult humans and 99% in 93 normal c ...	1999	10473521
bacterial expression and functional characterization of a rat thyroid hormone sulfotransferase, st1b1.	at least three forms of phenol sulfotransferase (st) st1b1, st1a1 and st1c1 are contained in rat livers. to identify the form contributing to the metabolism of 3,3',5-triiodothyronine (t3), functional characterization of these forms was performed by expression in escherichia coli. st1b1 and st1c1 were shown to be active on sulfation towards t3 with high affinity (km: 44.4 and 25.8 microm, respectively), whereas st1a1 had low affinity. in western blotting using antibodies raised against the indiv ...	1999	10361887
characterization of the bacillus macerans cyclodextrin glucanotransferase overexpressed in escherichia coli.	cyclodextrin glucanotransferase (cgtase, ec 2. 4. 1. 19) converts starch and related alpha-1,4-glucans to cyclodextrin (cd). our previous studies of the enzyme have suggested that e344 on the polypeptide is crucial to the enzyme activity. mutational analysis of cgtase was performed to confirm this idea. three mutant cgtases containing either e344d, e344k or e344l substitution were overexpressed in escherichia coli. however, only the wild-type and e344d cgtases became soluble when expressed at 20 ...	1999	10420654
role of the c-terminal extremities of the smooth muscle myosin heavy chains: implication for assembly properties.	the two light meromyosin isoforms from rabbit smooth muscle were prepared as recombinant proteins in escherichia coli. these species which differed only by their c-terminal extremity showed the same circular dichroism spectra and endotherms in measurements of differential scanning calorimetry. their solubility properties were different at ph 7.0 in the absence of monovalent salts. their paracrystals formed at low ph differed by their aspect and number. these data suggest a role for the c-termina ...	1999	10431827
dynamic measurement of myosin light-chain-domain tilt and twist in muscle contraction.	a new method is described for measuring motions of protein domains in their native environment on the physiological timescale. pairs of cysteines are introduced into the domain at sites chosen from its static structure and are crosslinked by a bifunctional rhodamine. domain orientation in a reconstituted macromolecular complex is determined by combining fluorescence polarization data from a small number of such labelled cysteine pairs. this approach bridges the gap between in vitro studies of pr ...	1999	10440371
thermal unfolding of phosphorylating d-glyceraldehyde-3-phosphate dehydrogenase studied by differential scanning calorimetry.	thermal unfolding parameters were determined for a two-domain tetrameric enzyme, phosphorylating d-glyceraldehyde-3-phosphate dehydrogenase (gapdh), and for its isolated nad(+)-binding domain. at ph 8.0, the transition temperatures (t(max)) for the apoforms of the native bacillus stearothermophilus gapdh and the isolated domain were 78.3 degrees c and 61.9 degrees c, with calorimetric enthalpies (deltah(cal)) of 4415 and 437 kj/mol (or 30.7 and 22.1 j/g), respectively. in the presence of nearly ...	1999	10446379
facile characterization of translation initiation via nonsense codon suppression.	a new strategy for studying the mechanism of translation initiation in eukaryotes has been developed. the strategy involves the use of an in vitro translation system to incorporate a non-natural fluorescent amino acid into a protein from a suppressor trnaphecua misacylated with that amino acid. it is thereby possible to monitor translation initiation efficiency at an aug codon in different contexts; this is illustrated for three constructs encoding escherichia coli dihydrofolate reductase mrna w ...	1999	10454635
induction of cellular immunosuppression by the human papillomavirus type 16 e7 oncogenic protein.	the human papillomavirus type 16 (hpv-16) e7 oncogenic protein is found in the culture supernatant of siha cells, a cervical carcinoma cell line. extracellular e7 protein, acting as a viral toxin in human immune cells, induces the overproduction of the immune suppressive ifn alpha cytokine by apcs, and inhibits the t-cell response to recall and allogenic antigens. these effects should be taken into account for the design of anti-human cervical carcinoma vaccines.	1999	10472433
identification and structural analysis of a mdv gene encoding a protein kinase.	dna sequence analysis of the bamhi-c fragment of marek's disease virus (mdv) reveals the presence of a 513 amino acid open reading frame (orf). this orf codes for a protein with an estimated m(r) of 58,901. comparison of the amino acid sequence with those available in the swiss-prot database indicates extensive homology with a protein kinase (pk) of herpes simplex virus (hsv) and varicella-zoster virus (vzv). in northern blot hybridization, a transcript of 2.0 kb was detected in mdv (ga strain) ...	1999	10696441
adenovirus-mediated suicide gene transduction: feasibility in lens epithelium and in prevention of posterior capsule opacification in rabbits.	the most common complication of cataract surgery is the development of posterior capsule opacification (pco). hyperplasia of the lens epithelium is one of the main cellular events following phacoemulsification, and has been found to be an important feature contributing to opacification of the posterior capsule. adenoviral vector-mediated transfer is a suitable method for transducing the herpes simplex virus thymidine kinase gene (hsv-tk) into proliferating cells, allowing for the selective killi ...	1999	10515456
metabolism of benzo[a]pyrene to trans-7,8-dihydroxy-7, 8-dihydrobenzo[a]pyrene by recombinant human cytochrome p450 1b1 and purified liver epoxide hydrolase.	recombinant human enzymes expressed in membranes obtained from escherichia coli transformed with cytochrome p450 (p450) and nadph-p450 reductase cdnas were used to identify the human p450 enzymes that are most active in catalyzing the oxidative transformation of benzo[a]pyrene in vitro. activation of benzo[a]pyrene to genotoxic products that cause induction of umu gene expression in salmonella typhimurium nm2009 by p450 1a1 and p450 1b1 enzymes was found to be enhanced by inclusion of purified e ...	1999	10409402
fractionation of polyclonal antibodies to fragments of a neuroreceptor using three increasingly chaotropic solvents.	we have developed specific antibodies against fragments of anaplastic lymphoma kinase (alk) in order to develop tools for characterizing the expression and biological function of this orphan receptor. the first fragment consisted of residues 280 to 480 of the murine extracellular domain, was expressed in escherichia coli (e. coli), purified in the presence of urea from the pellet of mechanically lysed cells and injected into rabbits as an unfolded protein in urea. the second fragment consisted o ...	1999	10379656
distribution of two isoforms of nadp-dependent isocitrate dehydrogenase in soybean (glycine max).	two different cdnas that encode nadp-specific isocitrate dehydrogenase (nadp-idh) isozymes of soybean (glycine max) were characterized. the nucleotide sequences of the coding regions of these cdnas have 74% identity to each other and give predicted amino acid sequences that have 83% identity to each other. using pcr techniques, their coding regions were subcloned into a protein overexpression vector, pqe32, to yield pidh4 and pidh1, respectively. both idh4 and idh1 enzymes were expressed in esch ...	1999	10394941
purification and characterization of chitin-binding proteins from the hemolymph of sweet potato hornworm, agrius convolvuli.	three chitin-binding proteins (cbps: cbp9, cbp15, cbp66) were identified from the larval hemolymph of sweet potato hornworm, agrius convolvuli. two (cbp9 and cbp15) of them have been isolated and purified by gel filtration (superdex hr 75), cation-exchange chromatography (mono s), and reverse-phase chromatography (murpc pc 2.1/3). in experiments to detect cbps in hemolymph, we examined whether ionic strength and existence of bovine serum albumin in the incubation solution influenced binding affi ...	1999	10665375
ca2+ sensitization and potentiation of the maximum level of myofibrillar atpase activity caused by mutations of troponin t found in familial hypertrophic cardiomyopathy.	human wild-type cardiac troponin t, i, c and five troponin t mutants (i79n, r92q, f110i, e244d, and r278c) causing familial hypertrophic cardiomyopathy were expressed in escherichia coli, and then were purified and incorporated into rabbit cardiac myofibrils using a troponin exchange technique. the ca2+-sensitive atpase activity of these myofibrillar preparations was measured in order to examine the functional consequences of these troponin mutations. an i79n troponin t mutation was found to cau ...	1999	10085122
rp463: a stabilized technetium-99m complex of a hydrazino nicotinamide derivatized chemotactic peptide for infection imaging.	a hynic-conjugated chemotactic peptide (fmlfk-hynic) was labeled with (99m)tc using tricine and tppts as coligands. the combination of fmlfk-hynic, tricine, and tppts with (99m)tc produced a ternary ligand complex [(99m)tc(fmlfk-hynic)(tricine)(tppts)] (rp463). rp463 was synthesized either in two steps, in which the binary ligand complex [(99m)tc(fmlfk-hynic)(tricine)(2)] (rp469) was formed first and then reacted with tppts, or in one step by direct reduction of [(99m)tc]pertechnetate with stann ...	1999	10502357
cloning and molecular characterization of plasmid-encoded antigens of borrelia burgdorferi.	thirteen independent clones that encode borrelia burgdorferi antigens utilizing antiserum from infection-immune rabbits were identified. the serum was adsorbed against noninfectious b. burgdorferi b31 to enrich for antibodies directed against either infection-associated antigens of b. burgdorferi b31 or proteins preferentially expressed during mammalian infection. the adsorption efficiency of the immune rabbit serum (irs) was assessed by western immunoblot analysis with protein lysates derived f ...	1999	10456881
does bile of patients with acute gallstone pancreatitis cause pancreatic inflammatory lesions? a study of the pancreatic toxicity of choledochal secretions collected at ercp.	bile flow into the pancreatic duct has been proposed as the cause of acute biliary pancreatitis. however, the pancreatic toxicity of choledochal bile from patients with acute gallstone pancreatitis has not been studied.	1999	10425414
prevalence of virulence factors of escherichia coli strains isolated from diarrheic and healthy piglets after weaning.	this study determined the prevalence of f4, f5, f6, f17 and f41 fimbriae and the genes for feda (f18 fimbriae), lt and st enterotoxins, and shiga toxins stx1, stx2 and stx2e among e. coli isolated from 372 weaned pigs with diarrhea and 46 healthy pigs of the same age. agglutination tests showed that most isolates were negative for all five fimbrial antigens. the f4 antigen was found in 71 (19.1%) and the f5, f6, or f41 antigen was detected in 6.4% of isolates from diseased pigs. genes for the f1 ...	1999	10510040
brainstem mechanisms of autonomic dysfunction in encephalopathy-associated shiga toxin 2 intoxication.	acute encephalopathy is the major determinant of death in an early stage of shiga toxin (stx)-producing escherichia coli infection. rapid progress toward refractory hypotension and dysfunction of breathing implies autonomic center dysfunction of patients. to clarify whether autonomic dysfunction becomes an ultimate cause of death in shiga toxemia, we injected purified stx2 (20 microg/kg) intravenously into rabbits, and monitored changes in cardiovascular and respiratory function together with re ...	1999	10360763
functional consequences of a carboxyl terminal missense mutation arg278cys in human cardiac troponin t.	a carboxyl terminal missense mutant arg278cys of human cardiac troponin t that causes familial hypertrophic cardiomyopathy was expressed in escherichia coli, purified, and exchanged into rabbit cardiac skinned muscle fibers using a troponin exchange technique. compared to the fibers exchanged with human cardiac wild-type troponin t, the fibers exchanged with the mutant arg278cys developed less maximum force with a decreased cooperativity and a slightly increased ca(2+) sensitivity, resulting in ...	1999	10405326
a novel mutant, apoa-i nichinan (glu235-->0), is associated with low hdl cholesterol levels and decreased cholesterol efflux from cells.	a novel variant of apolipoprotein (apo) a-i associated with low high density lipoprotein (hdl) cholesterolemia has been identified in a japanese family during screening for apoa-i variants by isoelectric focusing (ief) gel analysis. apoa-i (glu235-->0) nichinan was caused by a 3-bp deletion of nucleotides 1998 through 2000 in exon 4 of the apoa-i gene. four subjects in the family were heterozygous carriers for this mutation; the mean plasma concentrations of apoa-i and hdl cholesterol of affecte ...	1999	10364075
cloning and expression of a cell wall associated protein gene of mycobacterium tuberculosis.	genomic library of mycobacterium tuberculosis (sihv) was constructed into t7 promoter based vector pbluescript ii ks (+/-) in escherichia coil. the expression of mycobacterial antigens from recombinants was detected by immunoscreening using e-coli-preabsorbed hyperimmune rabbit anti-m tuberculosis cell wall associated proteins serum. among 980 clones tested by immunoscreening, a clone pjj5 was found to produce mycobacterial antigen. this clone was further characterized by immunoblotting and foun ...	1999	10639772
evaluation of commercially available escherichia coli j5 bacterin as protection against experimental challenge with pasteurella multocida in rabbits.	to evaluate the ability of commercially available escherichia coli j5 bacterin to protect rabbits from experimental challenge with pasteurella multocida.	1999	10407479
the effect of the lipid a analog, e5531 on fever induced by endotoxin from escherichia coli.	e5531 is a specific lipid a antagonist and is expected to be a drug for the treatment of septic shock. the lal (limulus amebocyte lysate) activity and pyrogenicity of e5531 were determined. the lal activity of e5531 is large and confirmed that e5531 had a high affinity to the lipopolysaccharide receptor. the pyrogenic activity of e5531 is weak and the pyrogenic profile of e5531 is different from that of usp (united states pharmacopoeia) reference standard endotoxin (etx). e5531 suppressed the py ...	1999	10328569
molecular cloning, characterization, and expression of a novel human neutral sphingomyelinase.	neutral sphingomyelinase (n-smase) has emerged as an important cell membrane-associated enzyme that participates in several signal transduction and cell regulatory phenomena. using expression cloning, we have identified a 3.7-kilobase pair cdna transcript for n-smase whose open reading frame predicts a 397-amino acid polypeptide. transfection of cos-7 cells with cdna for n-smase resulted in a marked increase in n-smase activity. recombinant n-smase (r-n-smase) had the following physical-chemical ...	1999	10601312
[western blotting of rstv gene products in rice and insects].	the ns3 and nc protein genes encoded by rna3 of rstv, the ncp and nsvc4 protein genes encoded by rna4 were subcloned into the e. coli expression vector pgex3x to express four groups of fusion protein under iptg induction. these fusion proteins were used to immunize rabbits to raise antisera. the antisera against the e. coli-expressed proteins were available for probing the presence of the viral gene products in both rice plant and insect hosts. the expected gene products can be probed only in di ...	1999	10665229
molecular cloning and characterization of a birch pollen minor allergen, bet v 5, belonging to a family of isoflavone reductase-related proteins.	birch pollen is a major cause of pollinosis and is responsible for cross-reactive oral allergies to fruits, nuts, and vegetables. the major allergen, bet v 1, has been extensively characterized, and 3 minor allergens, bet v 2, bet v 3, and bet v 4, have been cloned and sequenced. recently, another birch pollen protein with an apparent mass of 35 kd was described as a new ige-binding protein in birch pollen with cross-reacting homologues in plant foods.	1999	10550744
formulation of a neutral solution of ciprofloxacin upon complexation with aluminum.	clear solutions of 0.5 and 1.0% ciprofloxacin (cf) of ph 7.2 were prepared by the addition of aluminum chloride hexahydrate (alcl3.6h2o) in the molar proportion cf:alcl3.6h2o (3:1). minimum inhibitory concentrations (mic) of these solutions were the same as an equimolar solution of cf.hcl. solutions exhibited good physical, chemical and microbiological stability and satisfactorily overcame an ocular irritation test on rabbits.	1999	10668175
autoantibodies to brain components and antibodies to acinetobacter calcoaceticus are present in bovine spongiform encephalopathy.	bovine spongiform encephalopathy (bse) is a neurological disorder, predominantly of british cattle, which belongs to the group of transmissible spongiform encephalopathies together with creutzfeldt-jakob disease (cjd), kuru, and scrapie. autoantibodies to brain neurofilaments have been previously described in patients with cjd and kuru and in sheep affected by scrapie. spongiform-like changes have also been observed in chronic experimental allergic encephalomyelitis, at least in rabbits and guin ...	1999	10569779
construction and characterization of an equine herpesvirus 1 glycoprotein c negative mutant.	an equine herpesvirus 1 (ehv-1) strain racl 11 mutant was constructed that carries the escherichia coli lacz gene instead of the open reading frame encoding glycoprotein c (gc). the engineered virus mutant (l11(delta)gc) lacked codons 46-440 of the 1404 bp gene. on rabbit kidney cell line rk13 and equine dermal cell line edmin337, the l11(delta)gc virus grew to titers which were reduced by approximately 5- to 10-fold compared with wild-type racl11 virus or a repaired virus (r-l11(delta)gc). howe ...	1999	10082388
prevention of lethal acute hepatic failure by antimacrophage migration inhibitory factor antibody in mice treated with bacille calmette-guerin and lipopolysaccharide.	during the past few years, the biological functions of macrophage migration inhibitory factor (mif) have been extensively re-evaluated. this has been found to be protein involved in broad-spectrum pathophysiological states as an inflammatory cytokine, pituitary-derived hormone, and glucocorticoid-induced immunomodulator. in this study, we investigated the involvement of this cytokine in the pathogenesis of lethal liver injury. injecting a small dose of lipopolysaccharide (lps) into bacille calme ...	1999	10347118
isolation and purification of a campylobacter upsaliensis autolysin.	autolytic activity in the soluble and sediment fractions of sonicates of the spiral and the coccoid form of campylobacter upsaliensis could not be demonstrated by native (nondenaturing) polyacrylamide gel electrophoresis (page). autolysins were detected, however, by using denaturing sodium dodecyl sulfate (sds)-page gels containing either purified escherichia coli peptidoglycan or whole cells of micrococcus luteus (micrococcus lysodeikticus) as the turbid substrate, with subsequent renaturation ...	1999	10349717
ultrasonic enhancement of antibiotic action on escherichia coli biofilms: an in vivo model.	biofilm infections are a common complication of prosthetic devices in humans. previous in vitro research has determined that low-frequency ultrasound combined with aminoglycoside antibiotics is an effective method of killing biofilms. we report the development of an in vivo model to determine if ultrasound enhances antibiotic action. two 24-h-old escherichia coli (atcc 10798) biofilms grown on polyethylene disks were implanted subcutaneously on the backs of new zealand white female rabbits, one ...	1999	10223938
antigenic profile of african horse sickness virus serotype 4 vp5 and identification of a neutralizing epitope shared with bluetongue virus and epizootic hemorrhagic disease virus.	african horse sickness virus (ahsv) causes a fatal disease in horses. the virus capsid is composed of a double protein layer, the outermost of which is formed by two proteins: vp2 and vp5. vp2 is known to determine the serotype of the virus and to contain the neutralizing epitopes. the biological function of vp5, the other component of the capsid, is unknown. in this report, ahsv vp5, expressed in insect cells alone or together with vp2, was able to induce ahsv-specific neutralizing antibodies. ...	1999	10329555
domains of rinderpest virus phosphoprotein involved in interaction with itself and the nucleocapsid protein.	the yeast two-hybrid system was used to identify domains involved in specific in vivo interactions between the rinderpest virus (rpv) phosphoprotein (p) and nucleocapsid protein (n). n and p genes were cloned in both the yeast gal4 dna-binding and gal4 activation domain vectors, which enabled analysis of self and interprotein interactions. mapping of the domain of p protein involved in its association with itself revealed that the cooh-terminal 32 amino acids (316-347) that forms a part of the h ...	1999	10366579
growth phase-dependent regulation of vsr endonuclease may contribute to 5-methylcytosine mutational hot spots in escherichia coli.	using rabbit polyclonal antibodies, we have shown that the dcm cytosine methylase of escherichia coli is maintained at a constant level during cell growth, while vsr endonuclease levels are growth phase dependent. decreased production of vsr relative to dcm during the log phase may contribute substantially to the mutability of 5-methylcytosine.	1999	10400606
essential role of helicobacter pylori gamma-glutamyltranspeptidase for the colonization of the gastric mucosa of mice.	constitutive expression of gamma-glutamyltranspeptidase (ggt) activity is common to all helicobacter pylori strains, and is used as a marker for identifying h. pylori isolates. helicobacter pylori ggt was purified from sonicated extracts of h. pylori strain 85p by anion exchange chromatography. the n-terminal amino acid sequences of two of the generated endo-proteolysed peptides were determined, allowing the cloning and sequencing of the corresponding gene from a genomic h. pylori library. the h ...	1999	10200957
preparation and characterization of recombinant dolphin fish (coryphaena hippurus) growth hormone.	dolphin fish (coryphaena hippurus) growth hormone (dfgh) cdna encoding the mature protein was cloned in a pet11a expression vector and expressed in escherichia coli bl21 cells upon induction with isopropyl-1-thio-beta-d-galactopyranoside as an insoluble protein. the expressed protein, contained within the inclusion-body pellet, was solubilized in 4.5 m urea, refolded at ph 11.3 in the presence of catalytic amounts of cysteine, and purified to homogeneity, as evidenced by sds-page. gel filtration ...	1999	10425163