Publications

hos2 and hda1 encode histone deacetylases with opposing roles in candida albicans morphogenesis.	epigenetic mechanisms regulate the expression of virulence traits in diverse pathogens, including protozoan and fungi. in the human fungal pathogen candida albicans, virulence traits such as antifungal resistance, white-opaque switching, and adhesion to lung cells are regulated by histone deacetylases (hdacs). however, the role of hdacs in the regulation of the yeast-hyphal morphogenetic transitions, a critical virulence attribute of c. albicans, remains poorly explored. in this study, we wished ...	2010	20730094
marked amplification and diversification of products of ras genes from rat brain, rab gtpases, in the ciliates tetrahymena thermophila and paramecium tetraurelia.	small gtpase rab (products of ras genes from rat brain) is a widely conserved molecular switch among eukaryotes and regulates membrane trafficking pathways. it is generally considered that the number of rab encoded in the genome correlates with multicellularity; however, we found that unicellular ciliates tetrahymena thermophila (tt) and paramecium tetraurelia (pt) possess many more rab genes in their genome than the 64 hsrab genes in the human genome. we succeeded in isolating 86 cdna clones of ...	2010	20738463
regulation of serum response factor activity and smooth muscle cell apoptosis by chromodomain helicase dna-binding protein 8.	serum response factor (srf) is a widely expressed protein that plays a key role in the regulation of smooth muscle differentiation, proliferation, migration, and apoptosis. it is generally accepted that one mechanism by which srf regulates these diverse functions is through pathway-specific cofactor interactions. a novel srf cofactor, chromodomain helicase dna binding protein 8 (chd8), was isolated from a yeast two-hybrid screen using srf as bait. chd8 is highly expressed in adult smooth muscle ...	2010	20739623
involvement of tip60 acetyltransferase in intracellular salmonella replication.	salmonella enterica is a facultative intracellular pathogen that replicates within a membrane-bound compartment termed salmonella containing vacuole (scv). the biogenesis of scv requires salmonella type iii protein secretion/translocation system and their effector proteins which are translocated into host cells to exploit the vesicle trafficking pathways. ssef is one of these effectors required for scv formation and intracellular salmonella replication through unknown mechanisms.	2010	20796290
investigation of the estrogenic risk to feral male brown trout (salmo trutta) in the shannon international river basin district of ireland.	the estrogenic potential of sewage treatment effluents and their receiving waters in the shannon international river basin district (sirbd) of ireland was investigated. an integrated approach, combining biological and chemical methods, was conducted to assess 11 rivers adjacent to sewage treatment plants (stps) and their possible interference with the endocrine system of feral brown trout (salmo trutta). hepatosomatic index, gonadosomatic index, condition factor, histological (intersexuality) an ...	2010	20810166
a new twist in cellular resistance to the anticancer drug bleomycin-a5.	bleomycin is a potent chemotherapeutic agent that can mediate cell killing by attacking the dna. it is used in combination with other antineoplastic agents to effectively treat lymphomas, testicular carcinomas and squamous cell carcinomas of the cervix, head and neck. however, resistance to bleomycin remains a persistent limitation in exploiting the full therapeutic benefit of the drug for other types of cancers. herein, we review recent findings from both yeast and human cells showing that upta ...	2010	20812903
genome-wide functional annotation by integrating multiple microarray datasets using meta-analysis.	tremendous amounts of microarray data for various organisms have provided a rich opportunity for computational analyses of gene products. integrating these data can help inferring biological knowledge effectively. we present a new statistical method of integrating multiple microarray datasets for gene function prediction. we tested the performance of our model using yeast and human datasets. our results show that combining multiple datasets improves the accuracy over the best function prediction ...	2010	20815137
in vitro and in vivo enzymatic syntheses and mass spectrometric database for n-glycans and o-glycans.	in the glycogene project, we have comprehensively cloned novel human genes associated with the synthesis of glycans using bioinformatics technology. recombinant glycosyltransferases can be expressed in various expression systems. diverse glycan structures are easily and rapidly achieved using these glycosyltransferases in vitro. additionally, we have developed an in vivo production system for mammalian mucin-type glycopeptides using a genetically engineered yeast strain. this system enables the ...	2010	20816477
yeast proteomics and protein microarrays.	our understanding of biological processes as well as human diseases has improved greatly thanks to studies on model organisms such as yeast. the power of scientific approaches with yeast lies in its relatively simple genome, its facile classical and molecular genetics, as well as the evolutionary conservation of many basic biological mechanisms. however, even in this simple model organism, systems biology studies, especially proteomic studies had been an intimidating task. during the past decade ...	2010	20728591
formation of complex and unstable chromosomal translocations in yeast.	genome instability, associated with chromosome breakage syndromes and most human cancers, is still poorly understood. in the yeast saccharomyces cerevisiae, numerous genes with roles in the preservation of genome integrity have been identified. dna-damage-checkpoint-deficient yeast cells that lack sgs1, a recq-like dna helicase related to the human bloom's-syndrome-associated helicase blm, show an increased rate of genome instability, and we have previously shown that they accumulate recurring c ...	2010	20711256
construction of an in vitro trans-sialylation system: surface display of corynebacterium diphtheriae sialidase on saccharomyces cerevisiae.	sialidases can be used to transfer sialic acids from sialoglycans to asialoglycoconjugates via the trans-glycosylation reaction mechanism. some pathogenic bacteria decorate their surfaces with sialic acids which were often scavenged from host sialoglycoconjugates using their surface-localized enzymes. in this study, we constructed an in vitro trans-sialylation system by reconstructing the exogenous sialoglycoconjugate synthesis system of pathogens on the surfaces of yeast cells. the nanh gene en ...	2010	20711574
adhesion of yeast and bacteria to oral surfaces.	colonization of surfaces in the human body by microorganisms is an early, essential, step in the initiation of infectious disease. we have developed in vitro assays to investigate interactions between yeast or bacterial cells and human tissues, fluids, or prostheses. such assays can be used to identify the adhesins, ligands, and receptors involved in these interactions, for example by determining which components of the microbe or human tissue/fluid interfere with adherence in the assay. the ass ...	2010	20717781
autophagy, proteases and the sense of balance.	the knowledge of the molecular mechanisms underlying autophagy has considerably improved after the isolation and characterization of autophagy-defective mutants in the yeast saccharomyces cerevisiae. two ubiquitin-like conjugation systems are required for yeast autophagy. one of them requires the participation of atg8 synthesized as a precursor protein, which is cleaved after a gly residue by a cysteine proteinase called atg4. the new gly-terminal residue from atg8 is activated by atg7 (an e1-li ...	2010	20724821
rna silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga chlamydomonas reinhardtii.	the genome of the green alga chlamydomonas reinhardtii encodes two [fefe]-hydrogenases, hyda1 and hyda2, and the hydrogenase-like protein hyd3. the unique combination of these proteins in one eukaryotic cell allows for direct comparison of their in vivo functions, which have not been established for hyda2 and hyd3. using an artificial microrna silencing method developed recently, the expression of hyda1, hyda2 and hyd3 was specifically down-regulated. silencing of hyda1 resulted in 4-fold lower ...	2010	20726841
mutational analysis of nhaoc/nha2 in saccharomyces cerevisiae.	nhaoc/nha2 is highly and selectively expressed in osteoclasts and plays a role(s) in normal osteoclast differentiation, apoptosis and bone resorptive function in vitro. extensive mutational analysis of a bacterial homologue, nhaa, has revealed a number of amino acid residues essential for its activity. some of these residues are evolutionarily conserved and have been shown to be essential not only for activity of nhaa in bacteria, but also of nhaoc/nha2 in eukaryotes.	2010	20713131
plasmodium falciparum tudor staphylococcal nuclease interacting proteins suggest its role in nuclear as well as splicing processes.	tudor staphylococcal nuclease (p100 or snd1), a member of the micronuclease family is a multifunctional protein that plays a key role(s) in transcription and splicing processes in many eukaryotic cells. pftudor-sn, a plasmodium homolog of the human p100 protein is a structurally conserved protein; however molecular details of its function are not yet understood. our previous studies have shown that pftudor-sn binds rna and it is possible to selectively inhibit parasite growth by pftudor-sn speci ...	2010	20713134
the inflammatory response induced by aspartic proteases of candida albicans is independent of proteolytic activity.	the secretion of aspartic proteases (saps) has long been recognized as a virulence-associated trait of the pathogenic yeast candida albicans. in this study, we report that different recombinant saps, including sap1, sap2, sap3, and sap6, have differing abilities to induce secretion of proinflammatory cytokines by human monocytes. in particular sap1, sap2, and sap6 significantly induced interleukin-1β (il-1β), tumor necrosis factor alpha (tnf-α), and il-6 production. sap3 was able to stimulate th ...	2010	20713630
dietary modulation of drosophila sleep-wake behaviour.	a complex relationship exists between diet and sleep but despite its impact on human health, this relationship remains uncharacterized and poorly understood. drosophila melanogaster is an important model for the study of metabolism and behaviour, however the effect of diet upon drosophila sleep remains largely unaddressed.	2010	20706579
mumps virus small hydrophobic protein targets ataxin-1 ubiquitin-like interacting protein (ubiquilin 4).	the small hydrophobic (sh) protein of mumps virus has been reported to interfere with innate immunity by inhibiting tumour necrosis factor alpha-mediated apoptosis. in a yeast two-hybrid screen we have identified the ataxin-1 ubiquitin-like interacting protein (a1up) as a cellular target of the sh protein. a1up contains an amino-terminal ubiquitin-like (ubl) domain, a carboxy-terminal ubiquitin-associated (uba) domain and two stress-inducible heat shock chaperonin-binding (sti1) motifs. this pla ...	2010	20702650
use of a new gelling agent (eladium©) as an alternative to agar-agar and its adaptation to screen biofilm-forming yeasts.	the incidence of yeast-induced infections has increased in the last decade, mainly because of the increasing number of immunodeficient patients. since biofilm production is believed to be responsible for fungal virulence, we propose screening yeasts of various genera in order to determine their ability to form biofilms. this is an important issue because yeast cells that form biofilms are particularly resistant to anti-fungal agents used in human patients. for screening, we used eladium©, a new ...	2010	20703874
combination of sodium chlorite and calcium propionate reduces enzymatic browning and microbial population of fresh-cut "granny smith" apples.	tissue browning and microbial growth are the main concerns associated with fresh-cut apples. in this study, effects of sodium chlorite (sc) and calcium propionate (cp), individually and combined, on quality and microbial population of apple slices were investigated. "granny smith" apple slices, dipped for 5 min in cp solutions at 0%, 0.5%, 1%, and 2% (w/v) either alone or in combination with 0.05% (w/v) sc, were stored at 3 and 10 degrees c for up to 14 d. color, firmness, and microflora populat ...	2010	20492244
candida species isolated from the gastrointestinal tract of cockatiels (nymphicus hollandicus): in vitro antifungal susceptibility profile and phospholipase activity.	over the past years, the incidence of yeast infections, especially candidiasis, has increased. it is known that birds, including cockatiels, harbor potentially pathogenic yeasts to human beings in their gastrointestinal tract. thus, this work aims at determining the in vitro antifungal susceptibility and phospholipase activity of candida spp. isolated from the gastrointestinal tract and stools of cockatiels. sixty cockatiels were assessed and samples were collected from oral cavity, crop and clo ...	2010	20493645
transferrin fusion technology: a novel approach to prolonging biological half-life of insulinotropic peptides.	fusion proteins made up of glucagon-like peptide 1 (glp-1) and exendin-4 (ex-4) fused to a nonglycosylated form of human transferrin (glp-1-tf or ex-4-tf) were produced and characterized. glp-1-tf activated the glp-1 receptor, was resistant to inactivation by peptidases, and had a half-life of approximately 2 days, compared with 1 to 2 min for native glp-1. glp-1-tf retained the acute, glucose-dependent insulin-secretory properties of native glp-1 in diabetic animals and had a profound effect on ...	2010	20498254
upregulated expression of hitf in crohn's disease and screening of hitf interactant by a yeast two-hybrid system.	to study the expression of human intestinal trefoil factor (hitf) mrna in crohn's disease and to screen the cellular proteins that can interact with the hitf protein by a yeast two-hybrid system in order to explore the mechanism of hitf in protecting intestinal mucosa from injury.	2010	20499178
conditional cell-wall mutants of saccharomyces cerevisiae as delivery vehicles for therapeutic agents in vivo to the gi tract.	strains of saccharomyces cerevisiae capable of lysis upon conditional down-regulation of cell-wall biogenesis genes (srb1 and pkc1) have been reported. here, we show that they lyse and release recombinant protein not only under laboratory conditions, but (more importantly) under conditions found in the human stomach and duodenum. these findings provide proof that, in principle, such conditional lysis strains could be used as an integral part of a system for the oral delivery of therapeutic prote ...	2010	20356564
arabidopsis thaliana alg3 mutant synthesizes immature oligosaccharides in the er and accumulates unique n-glycans.	the core oligosaccharide glc(3)man(9)glcnac(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (er). the first step of this assembly pathway on the er luminal side is mediated by alg3 (asparagine-linked glycosylation 3), which is a highly conserved reaction among eukaryotic cells. complementary genetics compared with saccharomyces cerevisiae alg gene families and bioinformatic approaches have en ...	2010	20356820
fatp1 inhibits 11-cis retinol formation via interaction with the visual cycle retinoid isomerase rpe65 and lecithin:retinol acyltransferase.	the isomerization of all-trans retinol (vitamin a) to 11-cis retinol in the retinal pigment epithelium (rpe) is a key step in the visual process for the regeneration of the visual pigment chromophore, 11-cis retinal. lrat and rpe65 are recognized as the minimal isomerase catalytic components. however, regulators of this rate-limiting step are not fully identified and could account for the phenotypic variability associated with inherited retinal degeneration (rd) caused by mutations in the rpe65 ...	2010	20356843
application of stopped-flow kinetics methods to investigate the mechanism of action of a dna repair protein.	transient kinetic analysis is indispensable for understanding the workings of biological macromolecules, since this approach yields mechanistic information including active site concentrations and intrinsic rate constants that govern macromolecular function. in case of enzymes, for example, transient or pre-steady state measurements identify and characterize individual events in the reaction pathway, whereas steady state measurements only yield overall catalytic efficiency and specificity. indiv ...	2010	20357752
control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout.	in most of oviparous animals, vitellogenins (vtg) are the major egg yolk precursors. they are produced in the liver under the control of estrogens. in rainbow trout (oncorhynchus mykiss), the vtg genes cluster contains an unusually large number of almost identical gene copies. in order to identify the regulatory elements in their promoters, we used a combination of reporter plasmids containing genomic sequences including putative estrogen response elements (eres) and we performed transient trans ...	2010	20637911
14-3-3gamma mediates cdc25a proteolysis to block premature mitotic entry after dna damage.	14-3-3 proteins control various cellular processes, including cell cycle progression and dna damage checkpoint. at the dna damage checkpoint, some subtypes of 14-3-3 (beta and zeta isoforms in mammalian cells and rad24 in fission yeast) bind to ser345-phosphorylated chk1 and promote its nuclear retention. here, we report that 14-3-3gamma forms a complex with chk1 phosphorylated at ser296, but not at atr sites (ser317 and ser345). ser296 phosphorylation is catalysed by chk1 itself after chk1 phos ...	2010	20639859
secreted ph-regulated antigen 1 of candida albicans blocks activation and conversion of complement c3.	the complement system forms the first defense line of innate immunity and is activated within seconds upon infection by human pathogenic yeast candida albicans. in this study, we identified a new complement evasion strategy used by c. albicans. the fungus secretes a potent complement inhibitor, ph-regulated ag 1 (pra1), which in the direct surrounding of the pathogen binds to fluid-phase c3 and blocks cleavage of c3 to c3a and c3b, as shown by elisa, native gel electrophoresis, and western blott ...	2010	20644161
combining machine learning and homology-based approaches to accurately predict subcellular localization in arabidopsis.	a complete map of the arabidopsis (arabidopsis thaliana) proteome is clearly a major goal for the plant research community in terms of determining the function and regulation of each encoded protein. developing genome-wide prediction tools such as for localizing gene products at the subcellular level will substantially advance arabidopsis gene annotation. to this end, we performed a comprehensive study in arabidopsis and created an integrative support vector machine-based localization predictor ...	2010	20647376
targeting cell division cycle 7 kinase: a new approach for cancer therapy.	the cell division cycle 7 (cdc7) is a serine-threonine kinase, originally discovered in budding yeast, required to initiate dna replication. human cdc7 phosphorylates the minichromosome maintenance protein 2 (mcm2), a component of the dna replicative helicase needed for genome duplication. inhibition of cdc7 in cancer cells impairs progression through s phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability. small molecule compounds abl ...	2010	20647475
[screening and identification of interactive proteins of sh2d4a].	sh2d4a is a member of sh2 signaling protein family, which is involved in the signal transduction mediated by protein tyrosine kinase-related receptor, cell growth, proliferation, differentiation, and thereby affects the development of human disorders. to determine the role of sh2d4a in the cell signal transduction pathway, sh2d4a interactive proteins were screened using yeast two-hybrid system, and yeast mating and gst pull-down assays were carried out to further confirm the interaction. we succ ...	2010	20650852
the interaction between aid and cib1 is nonessential for antibody gene diversification by gene conversion or class switch recombination.	activation-induced deaminase (aid) initiates somatic hypermutation, gene conversion and class switch recombination by deaminating variable and switch region dna cytidines to uridines. aid is predominantly cytoplasmic and must enter the nuclear compartment to initiate these distinct antibody gene diversification reactions. nuclear aid is relatively short-lived, as it is efficiently exported by a crm1-dependent mechanism and it is susceptible to proteasome-dependent degradation. to help shed light ...	2010	20652029
divergent interactions involving the oxidosqualene cyclase and the steroid-3-ketoreductase in the sterol biosynthetic pathway of mammals and yeasts.	in mammals and yeasts, oxidosqualene cyclase (osc) catalyzes the formation of lanosterol, the first cyclic intermediate in sterol biosynthesis. we used a murine myeloma cell line (ns0), deficient in the 17β-hydroxysteroid dehydrogenase type 7 (hsd17b7), as a model to study the potential interaction of the hsd17b7 with the osc in mammals. hsd17b7 is the orthologue of the yeast steroid-3-ketoreductase (erg27), an enzyme of ergosterol biosynthesis that plays a protective role towards osc. tracer ex ...	2010	20659585
rad3 decorates critical chromosomal domains with gammah2a to protect genome integrity during s-phase in fission yeast.	schizosaccharomyces pombe rad3 checkpoint kinase and its human ortholog atr are essential for maintaining genome integrity in cells treated with genotoxins that damage dna or arrest replication forks. rad3 and atr also function during unperturbed growth, although the events triggering their activation and their critical functions are largely unknown. here, we use chip-on-chip analysis to map genomic loci decorated by phosphorylated histone h2a (gammah2a), a rad3 substrate that establishes a chro ...	2010	20661445
serine-409 phosphorylation and oxidative damage define aggregation of human protein tau in yeast.	unraveling the biochemical and genetic alterations that control the aggregation of protein tau is crucial to understand the etiology of tau-related neurodegenerative disorders. we expressed wild type and six clinical frontotemporal dementia with parkinsonism (ftdp) mutants of human protein tau in wild-type yeast cells and cells lacking mds1 or pho85, the respective orthologues of the tau kinases gsk3β and cdk5. we compared tau phosphorylation with the levels of sarkosyl-insoluble tau (sint), as ...	2010	20662935
shcd interacts with trkb via its ptb and sh2 domains and regulates bdnf-induced mapk activation.	neurotrophins regulate many aspects of neuronal function through activation of the high affinity trk receptors. shc family proteins are implicated in the coupling of rtk to the ras/mitogen-activated protein kinase signaling cascade. here we report that the fourth shc family member, shcd, associates with trkb receptor and regulates bdnf-induced mapk activation. yeast two-hybrid assay and co-ip experiments demonstrate shcd interacts with trkb in a kinase-activity-dependent manner. confocal analysi ...	2010	20663410
rapid identification of candida albicans in blood by combined capillary electrophoresis and fluorescence in situ hybridization.	a ce method based on whole-cell molecular labeling via fluorescence in situ hybridization was developed for the detection of candida albicans in whole blood. removal of potentially interfering red blood cells (rbc) with a simple hypotonic/detergent lysis step enabled us to detect and quantitate contaminating c. albicans cells at concentrations that were orders of magnitude lower than background rbc counts ( approximately 7.0 x 10(9) rbc/ml). in the presence of the lysed blood matrix, yeast cells ...	2010	20665522
characterization of the human hsc20, an unusual dnaj type iii protein, involved in iron-sulfur cluster biogenesis.	the importance of mitochondrial iron-sulfur cluster (isc) biogenesis for human health has been well established, but the roles of some components of this critical pathway still remain uncharacterized in mammals. among them is human heat shock cognate protein 20 (hhsc20), the putative human homolog of the specialized dnaj type co-chaperones, which are crucial for bacterial and fungal isc assembly. here, we show that the human hsc20 protein can complement for its counterpart in yeast, jac1p, and i ...	2010	20668094
identification of binding domains in the herpes simplex virus type 1 small capsid protein pul35 (vp26).	in this study, fragments of the small capsid protein pul35 (vp26) from herpes simplex virus type 1 (hsv-1) were generated to identify binding domains for a number of known ligands. analysis of the binding of dynein light chain subunits, dynlt1 and dynlt3, as well the hsv-1 structural proteins pul19 (vp5) and pul37 was then undertaken using the lexa yeast two-hybrid assay. the n-terminal half of pul35, in particular residues 30-43, was identified as a common region for the binding of dynlt1 and d ...	2010	20668116
identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase src homology domain 3 and alg-2-interacting protein x.	sfks (src family kinases) are central regulators of many signalling pathways. their functions are tightly regulated through sh (src homology) domain-mediated protein-protein interactions. a yeast two-hybrid screen using sh3 domains as bait identified alix [alg-2 (apoptosis-linked gene 2)-interacting protein x] as a novel hck (haemopoietic cell kinase) sh3 domain interactor. the alix-hck-sh3 interaction was confirmed in vitro by a gst (glutathione transferase) pull-down assay and in intact cells ...	2010	20670214
defining the pathway of cytoplasmic maturation of the 60s ribosomal subunit.	in eukaryotic cells the final maturation of ribosomes occurs in the cytoplasm, where trans-acting factors are removed and critical ribosomal proteins are added for functionality. here, we have carried out a comprehensive analysis of cytoplasmic maturation, ordering the known steps into a coherent pathway. maturation is initiated by the atpase drg1. downstream, assembly of the ribosome stalk is essential for the release of tif6. the stalk recruits gtpases during translation. because the gtpase ef ...	2010	20670889
the anaphase promoting complex activator ccs52a, a key factor for fruit growth and endoreduplication in tomato.	tomato fruit growth is characterized by the occurrence of numerous rounds of dna endoreduplication in connection to cell expansion and final fruit size determination. endoreduplication occurs as an impairment of mitosis, which can originate from the selective degradation of m-phase-specific cyclins via the ubiquitin-mediated proteolytic pathway, requiring the e3 ubiquitin ligase anaphase promoting complex/cyclosome (apc/c). in plants ccs52a is the ortholog of cdh1/fzr proteins from yeast, drosop ...	2010	20671429
ny-eso-1 protein glycosylated by yeast induces enhanced immune responses.	vaccine strategies that target dendritic cells to elicit potent cellular immunity are the subject of intense research. here we report that the genetically engineered yeast saccharomyces cerevisiae, expressing the full-length tumour-associated antigen ny-eso-1, is a versatile host for protein production. exposing dendritic cells (dcs) to soluble ny-eso-1 protein linked to the yeast a-agglutinin 2 protein (aga2p) protein resulted in protein uptake, processing and mhc class i cross-presentation of ...	2010	20672253
penicillium marneffei infection in an african man.	penicilliosis is a systemic fungal infection caused by penicillium marneffei. the infection is most commonly seen in southeast asia, southern china, hong kong, and taiwan. it is rarely seen among individuals of african descent. here, we report a case of penicilliosis in an african man from namibia who was studying in malaysia. he presented with multiple umbilicated papules associated with cough, fever, loss of appetite, and weight. he also had urethral discharge and admitted to unprotected sexua ...	2010	20673530
enterovirus type 71 2a protease functions as a transcriptional activator in yeast.	enterovirus type 71 (ev71) 2a protease exhibited strong transcriptional activity in yeast cells. the transcriptional activity of 2a protease was independent of its protease activity. ev71 2a protease retained its transcriptional activity after truncation of 40 amino acids at the n-terminus but lost this activity after truncation of 60 amino acids at the n-terminus or deletion of 20 amino acids at the c-terminus. thus, the acidic domain at the c-terminus of this protein is essential for its trans ...	2010	20682079
fission yeast telomeres forecast the end of the crisis.	recent years have placed fission yeast at the forefront of telomere research, as this organism combines a high level of conservation with human telomeres and precise genetic manipulability. here we highlight some of the latest knowledge of fission yeast telomere maintenance and dysfunction, and illustrate how principles arising from fission yeast research are raising novel questions about telomere plasticity and function in all eukaryotes.	2010	20682311
mannan-binding lectin deficiency results in unusual antibody production and excessive experimental colitis in response to mannose-expressing mild gut pathogens.	in crohn's disease (cd) the deficiency of mannan-binding lectin (mbl) is associated with an increased prevalence of anti-saccharomyces cerevisiae antibodies (asca) and with complicated phenotypes of the disease. however, the role of mbl in intestinal inflammation is currently unclear. a study was undertaken to analyse local mbl expression in human intestine and the consequences of mbl deficiency in experimental colitis and yeast infection.	2010	20682699
polyploidy: mechanisms and cancer promotion in hematopoietic and other cells.	polyploidy, the state of having greater than a diploid content of dna (e.g., tetraploid, octaploid, etc) has been recognized in a large variety of both, plant and animal cells. human and murine megakaryocytes, hepatocytes, arterial smooth muscle cells and cardiac myocytes, all develop a certain degree of polyploidy during their normal lifespan. in addition, polyploid cells may be found in some tissues under conditions of stress, including uterine smooth muscle during pregnancy, aortic vascular s ...	2010	20687472
genome-wide mapping of nuclear mitochondrial dna sequences links dna replication origins to chromosomal double-strand break formation in schizosaccharomyces pombe.	chromosomal double-strand breaks (dsbs) threaten genome integrity and repair of these lesions is often mutagenic. how and where dsbs are formed is a major question conveniently addressed in simple model organisms like yeast. numts, nuclear dna sequences of mitochondrial origin, are present in most eukaryotic genomes and probably result from the capture of mitochondrial dna (mtdna) fragments into chromosomal breaks. numt formation is ongoing and was reported to cause de novo human genetic disease ...	2010	20688779
the absence of genotoxicity of sucralose.	sucralose is a non-nutritive sweetener that is approximately 600 times sweeter than table sugar. it is currently approved for use in over 80 countries. evidence from chronic studies demonstrates that this compound is not carcinogenic. this report summarizes the results of genotoxicity studies that were part of the original safety assessment of sucralose-conducted early in the safety investigation and shared with regulatory agencies around the world. studies included the ames (salmonella typhimur ...	2010	20691239
interaction between nuclear localization signal-retinoic acid receptor alpha and ubiquilin 1.	to identify the interaction between nuclear localization signal-retinoic acid receptor alpha (nls-raralpha) and ubiquilin 1(ubqln1).	2010	20693704
post-translational control of ets transcription factors: detection of modified factors at target gene promoters.	ets transcription factors are implicated in gene regulation during cell proliferation and in the development of the haematopoietic cell lineage. characteristically, ets proteins act in concert with other transcription factors and are regulated by post-translational modifications, most frequently phosphorylation. these events have been shown to modulate the dna binding affinity and interactions of ets transcription factors with co-activators, events that can ultimately determine the formation of ...	2010	20694674
structure and function of sphingosine-1-phosphate lyase, a key enzyme of sphingolipid metabolism.	sphingosine-1-phosphate lyase (spl), a key enzyme of sphingolipid metabolism, catalyzes the irreversible degradation of sphingoid base phosphates. its main substrate sphingosine-1-phosphate (s1p) acts both extracellularly, by binding g protein-coupled receptors of the lysophospholipid receptor family, and inside the cell, as a second messenger. there, s1p takes part in regulating various cellular processes and its levels are tightly regulated. spl is a pivotal enzyme regulating s1p intracellular ...	2010	20696404
identification and functional characterization of the hpalg11 and the hprft1 genes involved in n-linked glycosylation in the methylotrophic yeast hansenula polymorpha.	the initial steps in n-linked glycosylation involve the synthesis of a lipid-linked core oligosaccharide followed by the transfer of the core glycan to nascent polypeptides in the endoplasmic reticulum (er). in this study, we have identified two genes, hpalg11and hprft1, in the metylotrophic yeast hansenula polymorpha. detailed analysis of the glycan structures of the n-linked glycans of secreted recombinant glucose oxidase in mutant strains hpalg3δ, hpalg11δ, and hpalg3δalg11δ with the assistan ...	2010	20696712
evolution of cst function in telomere maintenance.	telomeres consist of an elaborate, higher-order dna architecture, and a suite of proteins that provide protection for the chromosome terminus by blocking inappropriate recombination and nucleolytic attack. in addition, telomeres facilitate telomeric dna replication by physical interactions with telomerase and the lagging strand replication machinery. the prevailing view has been that two distinct telomere capping complexes evolved, shelterin in vertebrates and a trimeric complex comprised of cdc ...	2010	20697207
htlv-1 basic leucine-zipper factor, hbz, interacts with mafb and suppresses transcription through a maf recognition element.	htlv-1 infection causes adult t-cell leukemia (atl). the development of atl is thought to be associated with disruption of transcriptional control of cellular genes. htlv-1 basic leucine-zipper (bzip) factor, hbz, is encoded by the complementary strand of the provirus. we previously reported that hbz interacts with c-jun and suppresses its transcriptional activity. to identify the cellular factor(s) that interact with hbz, we conducted a yeast two-hybrid screen using full-length hbz as bait and ...	2010	20506502
identification of cysteine-rich epidermal growth factor-like domain 1alpha (creld1alpha) as a novel alpha1a-adrenoceptor-down-regulating protein and establishment of an alpha1l-adrenoceptor-expressing cell line.	two distinct alpha(1)-adrenoceptor phenotypes (alpha(1a)- and alpha(1l)-ars) are known to originate from a single adra1a(alpha(1a)) gene by an as-yet-unknown mechanism. we hypothesized that an alpha(1a)-ar-interacting protein could generate the alpha(1l)-ar phenotype and we sought to identify such a protein and to examine its effects on the expression of alpha(1a) and alpha(1l) phenotypes. cysteine-rich epidermal growth factor-like domain 1alpha (creld1alpha) was first identified using a yeast t ...	2010	20508391
duplicability of self-interacting human genes.	there is increasing interest in the evolution of protein-protein interactions because this should ultimately be informative of the patterns of evolution of new protein functions within the cell. one model proposes that the evolution of new protein-protein interactions and protein complexes proceeds through the duplication of self-interacting genes. this model is supported by data from yeast. we examined the relationship between gene duplication and self-interaction in the human genome.	2010	20509897
struct2net: a web service to predict protein-protein interactions using a structure-based approach.	struct2net is a web server for predicting interactions between arbitrary protein pairs using a structure-based approach. prediction of protein-protein interactions (ppis) is a central area of interest and successful prediction would provide leads for experiments and drug design; however, the experimental coverage of the ppi interactome remains inadequate. we believe that struct2net is the first community-wide resource to provide structure-based ppi predictions that go beyond homology modeling. a ...	2010	20513650
plasticity of human protein disulfide isomerase: evidence for mobility around the x-linker region and its functional significance.	protein disulfide isomerase (pdi), which consists of multiple domains arranged as abb'xa'c, is a key enzyme responsible for oxidative folding in the endoplasmic reticulum. in this work we focus on the conformational plasticity of this enzyme. proteolysis of native human pdi (hpdi) by several proteases consistently targets sites in the c-terminal half of the molecule (x-linker and a' domain) leaving large fragments in which the n terminus is intact. fluorescence studies on the w111f/w390f mutant ...	2010	20516074
bat3 promotes the membrane integration of tail-anchored proteins.	the membrane integration of tail-anchored proteins at the endoplasmic reticulum (er) is post-translational, with different tail-anchored proteins exploiting distinct cytosolic factors. for example, mammalian trc40 has a well-defined role during delivery of tail-anchored proteins to the er. although its saccharomyces cerevisiae equivalent, get3, is known to function in concert with at least four other components, get1, get2, get4 and get5 (mdy2), the role of additional mammalian proteins during t ...	2010	20516149
the fanconi anemia protein, fancg, binds to the ercc1-xpf endonuclease via its tetratricopeptide repeats and the central domain of ercc1.	there is evidence that fanconi anemia (fa) proteins play an important role in the repair of dna interstrand cross-links (icls), but the precise mechanism by which this occurs is not clear. one of the critical steps in the icl repair process involves unhooking of the cross-link from dna by incisions on one strand on either side of the icl and its subsequent removal. the ercc1-xpf endonuclease is involved in this unhooking step and in the removal of the cross-link. we have previously shown that se ...	2010	20518486
[polar steroids from solaster endeca starfish and the physiological activity of polar steroids from three starfish species].	four polyhydroxylated steroids, new (20r)-5alpha-cholestan-3beta,6alpha,8,15alpha,24,26-hexaol (i) and known (20r,25s)-5alpha-cholestan-3beta6alpha,8,15beta,16beta,26-hexaol, (20r,25s)-5alpha-cholestan-3beta,6alpha,15beta,16beta,26-pentaol, and marthasterone sulfate were isolated from the solaster endeca starfish inhabiting the sea of okhotsk and characterized. steroid (i) contains a 24,26-dihydroxylated side chain, which is uncommon for starfish polyols. the isolated steroids and related metabo ...	2010	20531484
molecular bridging of aging and cancer: a carf link.	collaborator of arf (carf) was first cloned as an arf partner in yeast two-hybrid screens. it enhances arf-dependent and -independent p53 functions, which are central to the control of cell growth and tumor suppression in human cells. carf interacts with arf, p53, and mdm2 proteins, and in turn gets regulated by mdm2-mediated degradation, suggesting a self-regulatory loop. carf is upregulated during replicative, oncogenic, and stress-induced senescence. overexpression of carf induced premature s ...	2010	20536841
the jmjn domain of jhd2 is important for its protein stability, and the plant homeodomain (phd) finger mediates its chromatin association independent of h3k4 methylation.	histone lysine methylation is a dynamic process that plays an important role in regulating chromatin structure and gene expression. recent studies have identified jhd2, a jmjc domain-containing protein, as an h3k4-specific demethylase in budding yeast. however, important questions regarding the regulation and functions of jhd2 remain unanswered. in this study, we show that jhd2 has intrinsic activity to remove all three states of h3k4 methylation in vivo and can dynamically associate with chroma ...	2010	20538609
predicting genetic modifier loci using functional gene networks.	most phenotypes are genetically complex, with contributions from mutations in many different genes. mutations in more than one gene can combine synergistically to cause phenotypic change, and systematic studies in model organisms show that these genetic interactions are pervasive. however, in human association studies such nonadditive genetic interactions are very difficult to identify because of a lack of statistical power--simply put, the number of potential interactions is too vast. one appro ...	2010	20538624
nutrient regulation of mtorc1 and cell growth.		2010	20543582
the c-terminal alpha-alpha superhelix of pat is required for mrna decapping in metazoa.	pat proteins regulate the transition of mrnas from a state that is translationally active to one that is repressed, committing targeted mrnas to degradation. pat proteins contain a conserved n-terminal sequence, a proline-rich region, a mid domain and a c-terminal domain (pat-c). we show that pat-c is essential for the interaction with mrna decapping factors (i.e. dcp2, edc4 and lsm1-7), whereas the p-rich region and mid domain have distinct functions in modulating these interactions. dcp2 and e ...	2010	20543818
alpha4 phosphoprotein interacts with edd e3 ubiquitin ligase and poly(a)-binding protein.	mammalian alpha4 phosphoprotein, the homolog of yeast tap42, is a component of the mammalian target-of-rapamycin (mtor) pathway that regulates ribogenesis, the initiation of translation, and cell-cycle progression. alpha4 is known to interact with the catalytic subunit of protein phosphatase 2a (pp2ac) and to regulate pp2a activity. using alpha4 as bait in yeast two-hybrid screening of a human k562 erythroleukemia cdna library, edd (e3 isolated by differential display) e3 ubiquitin ligase was id ...	2010	20544796
expression, purification and characterization of the cancer-germline antigen gage12i: a candidate for cancer immunotherapy.	gage cancer-germline antigens are frequently expressed in a broad range of different cancers, while their expression in normal tissues is limited to the germ cells of the immune privileged organs, testis and ovary. gage proteins are immunogenic in humans, which make them promising targets for immunotherapy and candidates for cancer vaccines. recombinant proteins may be superior to peptides as immunogens, since they have the potential to prime both cd4(+) and cd8(+) t cells and are not dependent ...	2010	20546897
the production, characterisation and enhanced pharmacokinetics of scfv-albumin fusions expressed in saccharomyces cerevisiae.	an expression system is described for the production of monomeric scfvs and scfv antibody fragments genetically fused to human albumin (at either the n- or c-terminus or both). based upon strains of saccharomyces cerevisiae originally developed for the production of a recombinant human albumin (recombumin) this system has delivered high levels of secreted product into the supernatant of shake flask and high cell density fed-batch fermentations. specific binding to the corresponding ligand was de ...	2010	20546898
the sarcomeric z-disc component myopodin is a multiadapter protein that interacts with filamin and alpha-actinin.	here we introduce myopodin as a novel filamin c binding partner. corroborative yeast two-hybrid and biochemical analyses indicate that the central part of myopodin that shows high homology to the closely related protein synaptopodin and that is common to all its currently known or predicted variants interacts with filamin c immunoglobulin-like domains 20-21. a detailed characterization of the previously described interaction between myopodin and alpha-actinin demonstrates for the first time that ...	2010	20554076
intramembrane proteolysis of mgm1 by the mitochondrial rhomboid protease is highly promiscuous regarding the sequence of the cleaved hydrophobic segment.	rhomboids are a family of intramembrane serine proteases that are conserved in bacteria, archaea, and eukaryotes. they are required for numerous fundamental cellular functions such as quorum sensing, cell signaling, and mitochondrial dynamics. mitochondrial rhomboids form an evolutionarily distinct class of rhomboids. it is largely unclear how their activity is controlled and which substrate determinants are responsible for recognition and cleavage. we investigated these requirements for the mit ...	2010	20558178
[lass2 interacts with v-atpase and inhibits cell growth of hepatocellular carcinoma].	homo sapiens longevity assurance homologue 2 (lass2) is a novel gene isolated from a human liver cdna library by our laboratory, and it is a human homologue of the yeast longevity assurance gene lag1 (saccharomyces cerevisiae longevity assurance gene). according to our previous results, lass2 could interact with subunit c of vacuolar type h(+)-atpase (v-atpase), and the overexpression of lass2 could inhibit the cell growth of a human hepatocellular carcinoma (hcc) cell line, smmc-7721. in order ...	2010	20571735
streptococcus mutans inhibits candida albicans hyphal formation by the fatty acid signaling molecule trans-2-decenoic acid (sdsf).	in the human mouth, fungi and several hundred species of bacteria coexist. here we report a case of interkingdom signaling in the oral cavity: a compound excreted by the caries bacterium streptococcus mutans inhibits the morphological transition from yeast to hyphae, an important virulence trait, in the opportunistic fungus candida albicans. the compound excreted by s. mutans was originally studied because it inhibited signaling by the universal bacterial signal autoinducer-2 (ai-2), determined ...	2010	20572249
the cellular rna helicase ddx1 interacts with coronavirus nonstructural protein 14 and enhances viral replication.	the involvement of host proteins in the replication and transcription of viral rna is a poorly understood area for many rna viruses. for coronaviruses, it was long speculated that replication of the giant rna genome and transcription of multiple subgenomic mrna species by a unique discontinuous transcription mechanism may require host cofactors. to search for such cellular proteins, yeast two-hybrid screening was carried out by using the nonstructural protein 14 (nsp14) from the coronavirus infe ...	2010	20573827
the closely related rna helicases, uap56 and urh49, preferentially form distinct mrna export machineries and coordinately regulate mitotic progression.	nuclear export of mrna is an essential process for eukaryotic gene expression. the trex complex couples gene expression from transcription and splicing to mrna export. sub2, a core component of the trex complex in yeast, has diversified in humans to two closely related rna helicases, uap56 and urh49. here, we show that urh49 forms a novel urh49-cip29 complex, termed the arex (alternative mrna export) complex, whereas uap56 forms the human trex complex. the mrnas regulated by these helicases are ...	2010	20573985
estrogenic pyrethroid pesticides regulate expression of estrogen receptor transcripts in mouse sertoli cells differently from 17beta-estradiol.	studies suggested that exposure to agricultural pesticides may affect male fertility. pyrethroids are widely used pesticides due to their insecticidal potency and low mammalian toxicity. a recombinant yeast assay system incorporating the human alpha-estrogen receptor was used to analyze the estrogenicity of a range of readily available pyrethroid pesticides. the commercial product ripcord plus showed estrogenic activity by this assay. to determine whether pyrethroid compounds might exert an effe ...	2010	20574910
long-term conservation of hcv rna at 4 degrees c using a new rna stabilizing solution.	protecting rna from degradation, whilst maintaining its biological activity, is essential in molecular biology. however, rna is very sensitive to degradation by ribonucleases, especially at temperatures above 0 degrees c. the stability of rna was examined at 4 degrees c and -20 degrees c, in a new stabilizing solution consisting of a low-molarity mixture of chaotropic agents guanidinium and ammonium thiocyanate, a buffer for ph stabilization, phenol, and yeast rna. two substrates were tested for ...	2010	20576506
the genemania prediction server: biological network integration for gene prioritization and predicting gene function.	genemania (http://www.genemania.org) is a flexible, user-friendly web interface for generating hypotheses about gene function, analyzing gene lists and prioritizing genes for functional assays. given a query list, genemania extends the list with functionally similar genes that it identifies using available genomics and proteomics data. genemania also reports weights that indicate the predictive value of each selected data set for the query. six organisms are currently supported (arabidopsis thal ...	2010	20576703
simple is good: yeast models of neurodegeneration.	the budding yeast, saccharomyces cerevisiae, is the best-studied eukaryotic cell, at both genetic and physiological levels. as a eukaryote, yeast shares highly conserved molecular and cellular mechanisms with human cells. thus, this simple fungus is an invaluable model to study the fundamental molecular mechanisms involved in several human diseases. in the particular case of neurodegenerative disorders, yeast models have been able to recapitulate several important features of complex and devasta ...	2010	20579105
recombinant form of human wild type mannan-binding lectin (mbl/a) but not its structural variant (mbl/c) promotes phagocytosis of zymosan by activating complement.	mannan-binding lectin (mbl) mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. in the present study, employing recombinant forms of human mbl (rmbl), the role of wild type mbl (rmbl/a) and its structural variant rmbl/c in mediating thp-1 phagocytosis of fluorescent-labeled zymosan was examined and compared to mbl purified from human plasma (pmbl/a). flow cytometric analyses revealed that opsonization of zymosan with r ...	2010	20579738
antimicrobial activity of mucosal-associated invariant t cells.	mucosal-associated invariant t lymphocytes (mait lymphocytes) are characterized by two evolutionarily conserved features: an invariant t cell antigen receptor (tcr) alpha-chain and restriction by the major histocompatibility complex (mhc)-related protein mr1. here we show that mait cells were activated by cells infected with various strains of bacteria and yeast, but not cells infected with virus, in both humans and mice. this activation required cognate interaction between the invariant tcr and ...	2010	20581831
synergy of caspofungin with human polymorphonuclear granulocytes for killing candida albicans.	the influence of caspofungin on polymorphonuclear leukocyte (pmn) phagocytosis and intracellular killing of candida albicans was investigated. caspofungin, at all of the concentrations tested (2, 3.2, and 8 microg/ml), significantly increased intracellular killing by pmns through its direct action on both yeast cells and pmns, indicating the potential ability of caspofungin to synergize with phagocytes for candidal killing. caspofungin may therefore constitute an effective therapeutic option for ...	2010	20585121
engineering of glycosylation in yeast and other fungi: current state and perspectives.	with the increasing demand for recombinant proteins and glycoproteins, research on hosts for producing these proteins is focusing increasingly on more cost-effective expression systems. yeasts and other fungi are promising alternatives because they provide easy and cheap systems that can perform eukaryotic post-translational modifications. unfortunately, yeasts and other fungi modify their glycoproteins with heterogeneous high-mannose glycan structures, which is often detrimental to a therapeuti ...	2010	20585772
identification of the transactivation domain of the human fhl3.	four and a half lim domain protein 3 (fhl3) has the transactivation and repressor activity, and plays important roles in regulating the expression levels of various genes. in this study, fhl3 was proved to possess the auto-activation ability when constructed into the pgbkt7 plasmid (a gala dna-binding domain (bd) cloning vector of the yeast two-hybrid system) and transformed into yeast y190 cells. to determine the transactivation domain of fhl3, five mutants were constructed by sequentially dele ...	2010	20586194
functional characterization of aibp, a novel aurora-a binding protein in centrosome structure and spindle formation.	aurora-a is involved in chromosome alignment, centrosome maturation, mitotic spindle assembly and regards to an oncogene. aurora-a is also known to bind to several other proteins affecting its up-regulation or down-regulation and localization. however, how these different binding signals work together to regulate aurora-a is not properly known. to explore more aurora-a interacting proteins, the low-copy yeast two-hybrid screening using aurora-a as bait protein was performed. one novel gene, aibp ...	2010	20596670
structure-mutagenicity relationship of kaurenoic acid from xylopia sericeae (annonaceae).	kaurane diterpenes are considered important compounds in the development of new highly effective anticancer chemotherapeutic agents. genotoxic effects of anticancer drugs in non-tumour cells are of special significance due to the possibility that they induce secondary tumours in cancer patients. in this context, we evaluated the genotoxic and mutagenic potential of the natural diterpenoid kaurenoic acid (ka), i.e. (-)-kaur-16-en-19-oic acid, isolated from xylopia sericeae st. hill, using several ...	2010	20599626
a decade of systems biology.	systems biology provides a framework for assembling models of biological systems from systematic measurements. since the field was first introduced a decade ago, considerable progress has been made in technologies for global cell measurement and in computational analyses of these data to map and model cell function. it has also greatly expanded into the translational sciences, with approaches pioneered in yeast now being applied to elucidate human development and disease. here, we review the sta ...	2010	20604711
the emerging role of vhs domain-containing tom1, tom1l1 and tom1l2 in membrane trafficking.	the maintenance of cellular homeostasis and execution of regulatory mechanisms to dynamically govern various cellular processes require the correct delivery of proteins to their target subcellular compartments. it is estimated that over 30% of the proteins encoded by the human genome, projected to encode about 25 000 proteins and other macromolecules, are delivered to the secretory and endocytic pathways where movement of proteins between various compartments is primarily mediated by vesicles/ca ...	2010	20604899
the saccharomyces cerevisiae swi/snf complex can catalyze formation of dimeric nucleosome structures in vitro.	the swi/snf chromatin-remodeling complexes, human baf/pbaf and yeast rsc, can catalyze formation of stably altered dimeric forms of nucleosomes. however, the ability to create remodeled dimers has not yet been reported for the saccharomyces cerevisiae swi/snf complex. despite its similarity with the other swi/snf proteins, the yeast swi/snf complex features certain structural and functional differences. this raises the question of whether yswi/snf can in fact catalyze formation of dimeric nucleo ...	2010	20608642
purification of functional human cl(-)/hco(3)(-) exchanger, ae1, over-expressed in saccharomyces cerevisiae.	there is no high-resolution structure for the membrane domain of the human erythrocyte anion exchanger, ae1 (band 3). in this report, we have developed an expression and purification strategy for ae1 to be used in crystallization trials. saccharomyces cerevisiae strain bj5457 was transformed with an expression vector encoding the ae1 membrane domain (ae1md, amino acids 388-911), fused c-terminally to an epitope tag, corresponding to the nine c-terminal amino acids of rhodopsin. the fusion protei ...	2010	20609390
experimental annotation of the human pathogen candida albicans coding and noncoding transcribed regions using high-resolution tiling arrays.	compared to other model organisms and despite the clinical relevance of the pathogenic yeast candida albicans, no comprehensive analysis has been done to provide experimental support of its in silico-based genome annotation.	2010	20618945
relocation of the chromosomal passenger complex prevents mitotic checkpoint engagement at anaphase.	the mitotic checkpoint monitors the attachment of kinetochores to microtubules and delays anaphase onset until all sister kinetochores have become attached to opposite poles [1, 2]. correct bipolar attachment leads to kinetochore deformation and tension and satisfies the checkpoint [3-6]. what prevents mitotic checkpoint reactivation when sister centromeres are split and tension is lost at anaphase onset? aurora b kinase, the catalytic subunit of the chromosomal passenger protein complex (cpc) [ ...	2010	20619651
proteogenomics to discover the full coding content of genomes: a computational perspective.	proteogenomics has emerged as a field at the junction of genomics and proteomics. it is a loose collection of technologies that allow the search of tandem mass spectra against genomic databases to identify and characterize protein-coding genes. proteogenomic peptides provide invaluable information for gene annotation, which is difficult or impossible to ascertain using standard annotation methods. examples include confirmation of translation, reading-frame determination, identification of gene a ...	2010	20620248
a screening method tuned for mrna processing factors in human cells by evaluation of the luciferase reporter activity and the subcellular distribution of bulk poly(a)+ rna.	screening of mrna export factors in saccharomyces cerevisiae and drosophila melanogaster has identified a number of mrna processing factors involved in multiple mrna processing steps. however, only limited information is available on human cells. here we established a screening system searching for mrna processing factors in human cells by combining the luciferase reporter system and fluorescence in situ hybridization, which evaluates the nuclear/cytoplasmic distribution of bulk poly(a)+ rna. th ...	2010	20622428
the collagen v homotrimer [alpha1(v)](3) production is unexpectedly favored over the heterotrimer [alpha1(v)](2)alpha2(v) in recombinant expression systems.	collagen v, a fibrillar collagen with important functions in tissues, assembles into distinct chain associations. the most abundant and ubiquitous molecular form is the heterotrimer [alpha1(v)](2)alpha2(v). in the attempt to produce high levels of recombinant collagen v heterotrimer for biomedical device uses, and to identify key factors that drive heterotrimeric chain association, several cell expression systems (yeast, insect, and mammalian cells) have been assayed by cotransfecting the human ...	2010	20625483