Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| the plasmodium protein network diverges from those of other eukaryotes. | plasmodium falciparum is the pathogen responsible for over 90% of human deaths from malaria. therefore, it has been the focus of a considerable research initiative, involving the complete dna sequencing of the genome, large-scale expression analyses, and protein characterization of its life-cycle stages. the plasmodium genome sequence is relatively distant from those of most other eukaryotes, with more than 60% of the 5,334 encoded proteins lacking any notable sequence similarity to other organi ... | 2005 | 16267557 |
| helicobacter pylori and gastric epithelial cells: from gastritis to cancer. | helicobacter pylori is a spiral, gram-negative rod-shaped pathogen that attaches to gastric epithelial cells in the human stomach and is a causative agent of chronic active gastritis, peptic ulcer and neoplasia. h. pylori is one of the most common pathogens afflicting humans and is the major environmental factor in the development of gastric cancer increasing from 4 to 6 folds the risk of its development. several specific virulence factors are implicated in the mechanism of h. pylori infection l ... | 2005 | 16270519 |
| upregulation of cathepsin w-expressing t cells is specific for autoimmune atrophic gastritis compared to other types of chronic gastritis. | to investigate a pathophysiological role of cathepsin w (catw), a putative thiol-dependent cysteine protease, which is specifically expressed in cytotoxic lymphocytes, in different types of chronic inflammation of the gastric mucosa. | 2005 | 16273605 |
| is the hypoxia-inducible factor pathway important in gastric cancer? | tumour hypoxia is well recognised in oncology to be a key factor resulting in treatment resistance and poor prognosis. hypoxia leads to the expression of a number of gene products that are involved in tumour progression, invasion and metastasis formation. the most important of these proteins is thought to be hypoxia-inducible factor-1alpha (hif-1alpha), which appears to be a master regulator of the cellular response to hypoxia. hif-1alpha expression is associated with a poor prognosis and treatm ... | 2005 | 16290133 |
| [helicobacter pylori caga and shp-2 tyrosine phosphatase]. | 2005 | 16296318 | |
| no correlation of baba2 with vaca and caga genotypes of helicobacter pylori and grading of gastritis from peptic ulcer disease patients in brazil. | the baba2 gene, which encodes a blood-group antigen-binding adhesin that mediates attachment of helicobacter pylori to human lewis(b) antigens on gastric epithelial cells, has been associated with a higher risk of peptic ulcer and gastric cancer. the purpose of this study was to ascertain the frequency of baba2 genotype in h. pylori strains of patients with peptic ulcer and to correlate with other virulence factors. | 2005 | 16302986 |
| role of surfactant protein d (sp-d) in innate immunity in the gastric mucosa: evidence of interaction with helicobacter pylori lipopolysaccharide. | surfactant protein d (sp-d) is a collagenous glycoprotein, a collectin, which functions as a pathogen-associated molecular pattern (pamp) recognition receptor in the innate immune response. although originally identified in the lung as a component of surfactant, sp-d also occurs in the gastric mucosa at the luminal surface and within gastric pits of mucus-secreting cells. infection with the gastroduodenal pathogen helicobacter pylori up-regulates expression of sp-d in human patients with gastrit ... | 2005 | 16303091 |
| cloning of helicobacter pylori urease subunit b gene and its expression in tobacco (nicotiana tabacum l.). | vaccines produced by transgenic plants would have the potential to change the traditional means of production and inoculation of vaccines, and to reduce the cost of vaccine production. in the present study, an ureb antigen gene from a new helicobacter pylori strain zjc02 was cloned into the binary vector pbi121 which contains a camv35s promoter and a kanamycin resistance gene, and then transformed ureb into tobacco leaf-disc by agrobacterium-mediated method. a total of 50 regenerated plants with ... | 2005 | 16133345 |
| host epithelial interactions with helicobacter pylori: a role for disrupted gastric barrier function in the clinical outcome of infection? | infection of the human stomach with helicobacter pylori may develop into gastritis, ulceration, adenocarcinoma and mucosal lymphomas. the pathogenic mechanisms that determine the clinical outcome from this microbial-epithelial interaction remain poorly understood. an increasing number of reports suggests that disruptions of epithelial barrier function may contribute to pathology and postinfectious complications in a variety of gastrointestinal infections. the aim of this review is to critically ... | 2005 | 16151546 |
| molecular pathogenesis of mucosa-associated lymphoid tissue lymphoma. | extranodal marginal zone b-cell lymphomas of mucosa-associated lymphoid tissue (malt) type occur in a number of anatomic sites, but share overlapping morphologic and immunophenotypic features. helicobacter pylori infection has been identified as an etiologic factor in gastric malt lymphoma, and a growing list of other infectious organisms have recently been shown to be associated with malt lymphomas at other anatomic sites. although cause and effect has not been established for most of these inf ... | 2005 | 16155022 |
| deciphering host migrations and origins by means of their microbes. | mitochondrial dna and microsatellite sequences are powerful genetic markers for inferring the genealogy and the population genetic structure of animals but they have only limited resolution for organisms that display low genetic variability due to recent strong bottlenecks. an alternative source of data for deciphering migrations and origins in genetically uniform hosts can be provided by some of their microbes, if their evolutionary history correlates closely with that of the host. in this revi ... | 2005 | 16156803 |
| bioactivities of anastasia black (russian sweet pepper). | anastasia black (russian sweet pepper) of capsicum annuum l. var. angulosum mill. (solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of twenty-three fractions by silica gel or octadecylsilane (ods; c18) column chromatography. these extracts and fractions were investigated for their cytotoxicity, anti-human immunodeficiency virus (hiv), anti-helicobacter pylori (h. pylori), urease inhibition and multidrug r ... | 2005 | 16158935 |
| the -251t allele of the interleukin-8 promoter is associated with increased risk of gastric carcinoma featuring diffuse-type histopathology in chinese population. | persistent interleukin-8 (il-8) production contributes to chronic inflammation of the stomach. the proinflammatory il-1beta polymorphisms, which enhance the cytokine production, are associated with increased risk of gastric cancer. the -251a/t polymorphism of the il-8 promoter is involved in several human diseases. particularly, the -251a is associated with decreased risk of colorectal cancer. we aimed to determine whether the -251 allele resulting in high il-8 expression was associated with inc ... | 2005 | 16166417 |
| mutation and recombination in helicobacter pylori: mechanisms and role in generating strain diversity. | the pathogenic bacterium helicobacter pylori infects half of the human population and it is one of the most genetically diverse bacterial species known. the unusual combination of high frequencies of mutation and recombination events together with a very small size of dna fragments imported into the genome after recombination, contribute to its genetic variability. in this review article, we discuss the genetic variability of h. pylori, the mechanisms that generate diversity, and the potential r ... | 2005 | 16173496 |
| helicobacter pylori and the innate immune system. | since its discovery, helicobacter pylori surprises us by its ability for life-long chronic persistence, proliferation, and probably active adaptation in the unfavourable niche of the human stomach, without being eliminated by the defence systems of the human body. this minireview highlights recent developments about the interaction of h. pylori with the innate immune system, and makes a case that evasion and possibly suppression of innate immune responses play an important role for the active su ... | 2005 | 16173499 |
| the oxysterol-binding protein homologue orp1l interacts with rab7 and alters functional properties of late endocytic compartments. | orp1l is a member of the human oxysterol-binding protein (osbp) family. orp1l localizes to late endosomes (les)/lysosomes, colocalizing with the gtpases rab7 and rab9 and lysosome-associated membrane protein-1. we demonstrate that orp1l interacts physically with rab7, preferentially with its gtp-bound form, and provide evidence that orp1l stabilizes gtp-bound rab7 on les/lysosomes. the rab7-binding determinant is mapped to the ankyrin repeat (ank) region of orp1l. the pleckstrin homology domain ... | 2005 | 16176980 |
| acid-induced activation of the urease promoters is mediated directly by the arsrs two-component system of helicobacter pylori. | the nickel-containing enzyme urease is an essential colonization factor of the human gastric pathogen helicobacter pylori which enables the bacteria to survive the low-ph conditions of the stomach. transcription of the urease genes is positively controlled in response to increasing concentrations of nickel ions and acidic ph. here we demonstrate that acid-induced transcription of the urease genes is mediated directly by the arsrs two-component system. footprint analyses identify binding sites of ... | 2005 | 16177315 |
| overexpression of caspase recruitment domain (card) membrane-associated guanylate kinase 1 (carma1) and card9 in primary gastric b-cell lymphoma. | although caspase recruitment domain (card) membrane-associated guanylate kinase (maguk) protein 1 (carma1) and card9 play important roles in lymphocyte activation, the significance of carma1 and card9 in the pathogenesis of gastric mucosa-associated lymphoid tissue (malt) lymphoma remains to be elucidated. | 2005 | 16177990 |
| helicobacter pylori and extragastric diseases--other helicobacters. | the involvement of helicobacter pylori in the pathogenesis of extragastric diseases continues to be an interesting topic in the field of helicobacter-related pathology. although conflicting findings have been reported for most of the disorders, a role of h. pylori seems to be important especially for the development of cardiovascular and hematologic disorders. previously isolated human and animal helicobacter sp. flexispira and "helicobacter heilmannii" strains have been validated using polyphas ... | 2005 | 16178972 |
| [pathogenesis of helicobacter pylori infection]. | helicobacter pylori (h. pylori), a long term colonizer of human stomach is known to infect a half of mankind. gastric and duodenal ulcer, gastric adenocarcinoma and malt lymphoma develop in a subset of infected individuals. pathogenesis of h. pylori infection is based on the long-term host to bacterial interaction and affected by the virulence factors of the bacterium, environmental and host factors (age, sex, blood type). mucosal inflammation is the basic principle mechanism underlying the dise ... | 2005 | 16179834 |
| h. pylori infection increases levels of exhaled nitrate. | helicobacter pylori infection is one of the most common chronic bacterial infections worldwide. despite the existence of a breath test for the diagnosis of h. pylori infection, no study has described the composition of volatile compounds, especially the levels of nitrate, in the exhaled air of patients with h. pylori infection. | 2005 | 16181348 |
| il1rn polymorphism and caga-positive helicobacter pylori strains increase the risk of duodenal ulcer in children. | duodenal ulcers in children are associated with helicobacter pylori gastric infection with caga-positive strains, but factors linked to the host are poorly known. the authors evaluated the role of proinflammatory interleukin-1 gene cluster polymorphisms in the pathogenesis of duodenal ulcer. they studied prospectively 437 children 1 to years old, 209 of whom were h. pylori positive and 228 of whom were h. pylori negative. il1b-511-c/t, -31t/c, and il1rn variable number of tandem repeats were gen ... | 2005 | 16183821 |
| helicobacter pylori-specific immune response in maternal serum, cord blood, and human milk among mothers with and without current helicobacter pylori infection. | we assessed the patterns of helicobacter pylori (h. pylori) specific maternal antibodies in maternal serum, cord blood, and milk, which might play a role in prevention of h. pylori infection because transferred to the infant. between november 2000 and november 2001, mothers were recruited after delivery of their offspring. h. pylori infection status was determined by 13c-urea breath test (ubt). specific h. pylori antibody profiles were analysed using commercial h. pylori-specific enzyme-linked i ... | 2005 | 16183830 |
| alteration of cytochrome p-450 and glutathione s-transferase activity in normal and malignant human stomach. | cytochrome p-450 and glutathione s-transferase (gst) activities were investigated in stomach tumor and tumor-adjacent tissues of patients (n = 211) with gastric adenocarcinoma, and in the stomach tissues of unaffected individuals (normal tissues, n = 113). a significant reduction in total cytochrome p-450 activity was observed in tumor and tumor-adjacent tissues versus normal stomach. in all cases, cytochrome p-450 activity was significantly higher in males than in females. in the case of smoker ... | 2005 | 16195217 |
| identification of non-helicobacter pylori spiral organisms in gastric samples from humans, dogs, and cats. | tightly coiled bacteria are a rare cause of gastric pathology in humans and represent a mixture of species for which a zoonotic origin is suspected. similar organisms are common inhabitants of the gastric mucosae of carnivores and pigs. it was the goal of the present study to determine the actual occurrence of each individual helicobacter species in human, canine, and feline stomachs in order to better understand the possible zoonotic significance. gastric biopsy samples from humans with histolo ... | 2005 | 15872252 |
| synergism of helicobacter pylori infection and stress on the augmentation of gastric mucosal damage and its prevention with alpha-tocopherol. | despite evidence that helicobacter pylori (h. pylori) infection is closely associated with stress in gastric ulcer patients, the underlying mechanism why ulcer recurrence after stress is augmented especially in patients with h. pylori remains unknown. in this study, we found that oxidative stress played a critical role in the augmented mucosal damage provoked by water immersion restraint stress (wirs) in h. pylori infection and that an antioxidant, alpha-tocopherol, could ameliorate the aggravat ... | 2005 | 15890619 |
| iron-responsive regulation of the helicobacter pylori iron-cofactored superoxide dismutase sodb is mediated by fur. | maintaining iron homeostasis is a necessity for all living organisms, as free iron augments the generation of reactive oxygen species like superoxide anions, at the risk of subsequent lethal cellular damage. the iron-responsive regulator fur controls iron metabolism in many bacteria, including the important human pathogen helicobacter pylori, and thus is directly or indirectly involved in regulation of oxidative stress defense. here we demonstrate that fur is a direct regulator of the h. pylori ... | 2005 | 15901691 |
| increased risk of intestinal type of gastric adenocarcinoma in japanese women associated with long forms of ccttt pentanucleotide repeat in the inducible nitric oxide synthase promoter. | tandem repeat number polymorphism of a ccttt pentanucleotide in the promoter region of the inducible nitric oxide synthase gene (inos) and a polymorphism of the interleukin-1beta (il-1b) promoter at position -31 were analyzed in dna samples from 181 japanese control subjects and 158 gastric cancer patients, including 96 intestinal type and 62 diffuse type. an association between the intestinal type of gastric adenocarcinoma and higher promoter activity of the inos gene was found in women, especi ... | 2005 | 15617837 |
| mucosal foxp3-expressing cd4+ cd25high regulatory t cells in helicobacter pylori-infected patients. | helicobacter pylori chronically colonizes the stomach and duodenum and causes peptic ulcers or gastric adenocarcinoma in 10 to 20% of infected individuals. we hypothesize that the inability of patients to clear h. pylori infections is a consequence of active suppression of the immune response. here we show that h. pylori-infected individuals have increased frequencies of cd4(+) cd25(high) t cells in both the stomach and duodenal mucosa compared to uninfected controls. these cells have the phenot ... | 2005 | 15618192 |
| hla polymorphisms are associated with helicobacter pylori infected gastric cancer in a high risk population, china. | helicobacter pylori is one of the most common bacterial infections associated with an increased risk of gastric cancer, but its association with host factors, particularly polymorphisms of the immune response genes, such as human leukocyte antigen (hla) genes, is still unclear. to investigate the role of hla polymorphisms in the risk of gastric cancer among subjects with h. pylori infection, a case-control study involving 52 gastric cancer patients and 139 non-cancer controls was conducted in li ... | 2005 | 15650879 |
| helicobacter infection, chronic inflammation, and the development of malignancy. | chronic infection with helicobacter pylori affects approximately half the world and results in malignancy in a small subset of this population. although the frequency of infection in developed nations is falling with a resultant decline in h. pylori-associated peptic ulcer disease, gastric cancer remains the second major cause of cancer death worldwide, with h. pylori infection being a major attributable factor in the development of gastric cancer. this review provides an update on the pathogene ... | 2005 | 15687882 |
| the sialic acid binding saba adhesin of helicobacter pylori is essential for nonopsonic activation of human neutrophils. | infiltration of neutrophils and monocytes into the gastric mucosa is a hallmark of chronic gastritis caused by helicobacter pylori. certain h. pylori strains nonopsonized stimulate neutrophils to production of reactive oxygen species causing oxidative damage of the gastric epithelium. here, the contribution of some h. pylori virulence factors, the blood group antigen-binding adhesin baba, the sialic acid-binding adhesin saba, the neutrophil-activating protein hp-nap, and the vacuolating cytotoxi ... | 2005 | 15689619 |
| the effect of ascorbic acid on helicobacter pylori induced cyclooxygenase 2 expression and prostaglandin e2 production by gastric epithelial cells in vitro. | cyclooxygenase 2 (cox-2) is induced by the presence of helicobacter pylori (h. pylori) on the gastric mucosa as part of the inflammatory response; this results in the synthesis of prostaglandins that amplify the local inflammatory response. the presence of h. pylori inhibits the secretion of ascorbate into the gastric lumen. interestingly, ascorbate inhibits the growth of h. pylori and low dietary levels are associated with an increased risk of gastric adenocarcinoma. we therefore investigated t ... | 2005 | 15691319 |
| concomitant dosing of famotidine with a triple therapy increases the cure rates of helicobacter pylori infections in patients with the homozygous extensive metabolizer genotype of cyp2c19. | proton-pump inhibitors, such as lansoprazole, are metabolized in the liver by cyp2c19 and cannot inhibit acid sufficiently in homozygous extensive metabolizers of cyp2c19. | 2005 | 15710002 |
| intracellular vaca is a valuable marker to predict whether helicobacter pylori induces progressive atrophic gastritis that is associated with the development of gastric cancer. | vaca was histochemically stained in biopsy specimen and was intracellularly and mainly located in fundic gland area. it is recognized gastric atrophy was observed in the h. pylori-positive patients with intracellular vaca compared with others. the aim of study is to understand the relationship between intracellular vaca and the progression of gastric atrophy that is associated with gastric cancer. biopsy specimens and sera were obtained from 364 people in their 50s and 60s without gastric cancer ... | 2005 | 15712638 |
| mutational analysis of metronidazole resistance in helicobacter pylori. | metronidazole is one of a few antibiotics effective in eliminating helicobacter pylori infection of the human stomach. several chromosomal loci have been implicated in resistance to this drug. saturation transposon mutagenesis of the h. pylori genome revealed inactivation of the rdxa gene as uniquely able to confer metronidazole resistance. | 2005 | 15728938 |
| helicobacter pylori induces interleukin-8 secretion by toll-like receptor 2- and toll-like receptor 5-dependent and -independent pathways. | helicobacter pylori is an important human pathogen that causes gastritis and is strongly associated with gastric ulcers, gastric adenocarcinomas, and mucosa-associated lymphoid tissue lymphomas. in response to h. pylori, interleukin-8 (il-8) is secreted from host cells to attract components of the innate and adaptive immune systems to the site of infection. toll-like receptor 2 (tlr2) and tlr5 have been shown to recognize h. pylori and to initiate signaling pathways that result in enhanced activ ... | 2005 | 15731050 |
| rapid loss of motility of helicobacter pylori in the gastric lumen in vivo. | the human pathogen helicobacter pylori has infected more than half of the world's population. nevertheless, the first step of infection, the acute colonization of the gastric mucus, is poorly understood. for successful colonization, h. pylori must retain active motility in the gastric lumen until it reaches the safety of the mucus layer. to identify the factors determining the acute colonization, we inserted bacteria into the stomach of anesthetized mongolian gerbils. we adjusted the gastric jui ... | 2005 | 15731057 |
| colonization and inflammation deficiencies in mongolian gerbils infected by helicobacter pylori chemotaxis mutants. | helicobacter pylori causes disease in the human stomach and in mouse and gerbil stomach models. previous results have shown that motility is critical for h. pylori to colonize mice, gerbils, and other animal models. the role of chemotaxis, however, in colonization and disease is less well understood. two genes in the h. pylori chemotaxis pathway, chey and tlpb, which encode the chemotaxis response regulator and a methyl-accepting chemoreceptor, respectively, were disrupted. the chey mutation was ... | 2005 | 15731083 |
| genetic polymorphisms of cyp2e1, gstt1, gstp1, gstm1, aldh2, and odc and the risk of advanced precancerous gastric lesions in a chinese population. | there have been few studies of the associations of genetic polymorphisms with precancerous gastric lesions. we conducted a cross-sectional study to compare the prevalences of several genetic polymorphisms in 302 subjects with mild chronic atrophic gastritis with prevalences in 606 subjects with deep intestinal metaplasia or dysplasia. this stratified random sample of 908 subjects was selected and analyzed for genetic polymorphisms from 2,628 individuals who had gastric biopsies with histopatholo ... | 2005 | 15734972 |
| morphological changes in human gastric tumours after eradication therapy of helicobacter pylori in a short-term follow-up. | it is controversial as to whether the development of gastric cancer is influenced by helicobacter pylori eradication. if eradication itself influences the tumour morphology, this may affect the tumour discovery rate. | 2005 | 15740539 |
| polarized expression of cd74 by gastric epithelial cells. | cd74 is known as the major histocompatibility complex (mhc) class ii-associated invariant chain (ii) that regulates the cell biology and functions of mhc class ii molecules. class ii mhc and ii expression was believed to be restricted to classical antigen-presenting cells (apc); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class ii mhc molecules. given the importance of ii in the biology of class ii mhc, we sought to exami ... | 2005 | 15923369 |
| enhancing health benefits of berries through phenolic antioxidant enrichment: focus on cranberry. | emerging epidemiological evidence is increasingly pointing to the beneficial effects of fruits and vegetables in managing chronic and infectious diseases. these beneficial effects are now suggested to be due to the constituent phenolic phytochemicals having antioxidant activity. cranberry like other fruits is also rich in phenolic phytochemicals such as phenolic acids, flavonoids and ellagic acid. consumption of cranberry has been historically been linked to lower incidences of urinary tract inf ... | 2005 | 15927928 |
| helicobacter pylori promote gastric cancer cells invasion through a nf-kappab and cox-2-mediated pathway. | to examine the effects of helicobacter pylori (h pylori) infection on the invasiveness of gastric cancer cells, and to elucidate its mechanism. | 2005 | 15929167 |
| interleukin 12 gene polymorphisms enhance gastric cancer risk in h pylori infected individuals. | 2005 | 15937086 | |
| long-term persistence of molecular disease after histological remission in low-grade gastric malt lymphoma treated with h. pylori eradication. lack of association with translocation t(11;18): a 10-year updated follow-up of a prospective study. | localized low-grade gastric mucosa-associated lymphoid tissue (malt) lymphoma can regress after helicobacter pylori eradication, but igv(h) gene monoclonality may persist. we studied the long-term histological and molecular follow-up of 24 patients and the possible association of t(11;18) with the persistent monoclonality. | 2005 | 15946976 |
| alpha-catenin expression is decreased in patients with gastric carcinoma. | to assess the expression of alpha-catenin in gastric carcinoma and to determine the role of alpha-catenin expression in gastric carcinogenesis. | 2005 | 15948257 |
| comparative proteome analysis of untreated and helicobacter pylori-treated hepg2. | to investigate the pathological effect of helicobacter pylori (h pylori) on human hepatic cells, proteomic methods were used to find and to identify proteins that were overexpressed in hepg2 cells treated by h pylori. | 2005 | 15948260 |
| evasion of toll-like receptor 5 by flagellated bacteria. | toll-like receptor 5 (tlr5) recognizes an evolutionarily conserved site on bacterial flagellin that is required for flagellar filament assembly and motility. the alpha and epsilon proteobacteria, including the important human pathogens campylobacter jejuni, helicobacter pylori, and bartonella bacilliformis, require flagellar motility to efficiently infect mammalian hosts. in this study, we demonstrate that these bacteria make flagellin molecules that are not recognized by tlr5. we map the site r ... | 2005 | 15956202 |
| effect of cyp2c19 and mdr1 polymorphisms on cure rate in patients with acid-related disorders with helicobacter pylori infection. | a proton pump inhibitor (ppi) plus two antibiotics (amoxicillin and either clarithromycin or metronidazole) are recommended for treatment of acid-related disorders with helicobacter pylori (h. pylori) infection. the aim of this pharmacogenetic study was to evaluate the efficacy of triple therapy with ppis on eradication of h. pylori infection in relation to cytochrome p450 2c19 (cyp2c19) and p-glycoprotein (mdr1) gene polymorphisms. the retrospective study involved 70 polish caucasian patients w ... | 2005 | 15976989 |
| infection with helicobacter pylori affects all major secretory cell populations in the human antrum. | we have investigated how gastric h. pylori infection affects antrum secretory cell types by studying the expression of secretory proteins in antrum epithelium. antrum biopsy specimens were prospectively collected from 102 individuals (49 h. pylori-infected). immunohistochemistry was performed for secretory mucins (muc5ac, muc5b, muc6), trefoil factor family (tff)-peptides (tff1, tff2), endocrine peptides (gastrin, chromogranin a), and proliferating cells (ki-67). protein expression was quantifie ... | 2005 | 15986858 |
| [epidemiologic, pathologic, diagnostic and clinical features and therapy of helicobacter pylori infection based on latest data]. | helicobacter pylori is one of the most common human microorganisms. the outcome of its acquisition is not predictable, nevertheless a great amount of knowledge has accumulated concerning the virulence factors of the bacteria and the diseases associated with the infection. in most cases, this indigenous bacterium of the human stomach does not cause serious illnesses and the consequences of the infection depend on host and environmental factors. a more comprehensive insight into bacterial pathogen ... | 2005 | 15997663 |
| helicobacter pylori induce neutrophil transendothelial migration: role of the bacterial hp-nap. | continuous recruitment of neutrophils into the inflamed gastric mucosal tissue is a hallmark of helicobacter pylori infection in humans. in this study, we examined the ability of h. pylori to induce transendothelial migration of neutrophils using a transwell system consisting of a cultured monolayer of human endothelial cells as barrier between two chambers. we showed for the first time that live h. pylori, but not formalin-killed bacteria, induced a significantly increased transendothelial migr ... | 2005 | 16000239 |
| molecular cloning and functional expression of a novel helicobacter pylori alpha-1,4 fucosyltransferase. | helicobacter pylori is an important human pathogen which causes both gastric and duodenal ulcers and is associated with gastric cancer and lymphoma. this microorganism synthesizes fucosylated oligosaccharides, predominantly the galb-1,4glcnac (type ii) blood group antigens lewis x and y, whereas a small population also expresses the galb-1,3glcnac (type i) blood group antigens lewis a and b. these carbohydrate structures are known to mimic host cell antigens and permit the bacteria to escape fro ... | 2005 | 16000696 |
| hyperemesis gravidarum, a literature review. | hyperemesis gravidarum (hg) is a condition causing severe nausea and vomiting in early pregnancy often resulting in hospital admission. the incidence of hg is approximately 0.5% of live births, said to be higher in multiple pregnancies, hydatidiform mole and other conditions associated with increased pregnancy hormone levels. both the aetiology and pathogenesis of hg remain unknown. we conducted a literature review (1966-now) to summarize the current evidence on the aetiology and pathogenesis of ... | 2005 | 16006438 |
| human t-lymphotropic virus type i infection and idiopathic thrombocytopnic purpura. | human t-lymphotropic virus type i (htlv-i) is the causative agent in adult t-cell leukemia and htlv-i associated myelopathy. some other diseases such as uveitis, chronic thyroiditis, sjögren syndrome, arthritis, acute myeloid leukemia and myelodysplastic syndrome may be also associated with htlv-i. several case reports have suggested the possible combination of idiopathic thrombocytopenic purpura (itp) and htlv-i infection. in these studies and from our current report, we found 17 patients (22.1 ... | 2005 | 16019454 |
| synergistic enhancement of toll-like receptor responses by nod1 activation. | nod1 is an intracellular pattern-recognition receptor specific for gram-negative peptidoglycan that is important in host response to infections (e.g. helicobacter pylori and shigella flexneri). genetic variation in nod1 predisposes to asthma and inflammatory bowel disease. functional responses have not previously been studied in primary human cells. nod1 activation by low nanomolar concentrations of the specific muropeptide ligand m-tridap induced minimal human peripheral blood mononuclear cell ... | 2005 | 16021603 |
| up-regulation of cathepsin x in helicobacter pylori gastritis and gastric cancer. | recently, we identified increased cathepsin x expression in h. pylori-infected gastric mucosa. here, we describe further up-regulation in gastric cancer and report on the role of inflammatory cytokines required for cathepsin x up-regulation in h. pylori-infected gastric mucosa, as well as on consequences for cellular invasion. biopsy specimens were taken from the antrum, corpus and cardia of h. pylori-infected and non-infected patients. gastric cancer samples were obtained from patients undergoi ... | 2005 | 16025436 |
| microsomal prostaglandin e synthase (mpges)-1, mpges-2 and cytosolic pges expression in human gastritis and gastric ulcer tissue. | recently, three different prostaglandin e2 synthases have been identified: microsomal prostaglandin e synthase (mpges)-1, cytosolic pges (cpges), and mpges-2; however, their role and connection to cyclooxygenase (cox)-2 in the gastric ulcer repair process remain unknown. therefore, we examined mpges-1, cpges, and mpges-2 expression and localization in the stomach in vitro and in vivo. tissues were obtained from helicobacter pylori (h. pylori)-infected patients and consisted of surgical resection ... | 2005 | 15531909 |
| human peripheral and gastric lymphocyte responses to helicobacter pylori napa and aphc differ in infected and uninfected individuals. | in this study, we identify the nature of the immunological response of human peripheral blood mononuclear cells (pbmc) and lamina propria gastric lymphocytes (lpl) to two helicobacter pylori antigens, the neutrophil activating protein (napa) and alkyl hydroperoxide reductase (aphc). these antigens were identified and selected for study based on the observation that serological recognition of these proteins was associated with h pylori negative status in humans. | 2005 | 15591500 |
| the interaction of h. pylori infection and nsaids in cyclooxygenase-2 mrna expression in gastric antral, corpus mucosa, and gastric ulcer. | although helicobacter pylori infection and use of nonsteroidal anti-inflammatory drugs (nsaids) are the two major causes of gastric ulcer, their interaction remains controversial. we constructed a prospective cohort study to evaluate how these two factors influence the expression of cox-2 mrna in gastric antral, corpus mucosa, and gastric ulcer. | 2005 | 15599211 |
| novel binding epitope for helicobacter pylori found in neolacto carbohydrate chains: structure and cross-binding properties. | helicobacter pylori is a bacterium that colonizes the stomach of a majority of the global human population causing common gastric diseases like ulcers and cancer. it has an unusually complex pattern of binding to various host glycoconjugates including interaction with sialylated, sulfated, and fucosylated sequences. the present study describes an additional binding epitope comprising the neolacto internal sequence of glcnacbeta3-galbeta4glcnacbeta. the binding was detected on tlc plates as an in ... | 2005 | 15743770 |
| detection of helicobacter pylori dna in human faeces and water with different levels of faecal pollution in the north-east of spain. | to assess the role of water in the faecal transmission of helicobacter pylori by detecting the dna of this pathogen in human faecal samples and environmental water samples with a range of faecal pollution from the north-east of spain. | 2005 | 15752335 |
| towards a new therapeutic target: helicobacter pylori flavodoxin. | helicobacter pylori flavodoxin is the electronic acceptor of the pyruvate-oxidoreductase complex (por) that catalyzes pyruvate oxidative decarboxilation. inactivation of this metabolic route precludes bacterial survival. because flavodoxin is not present in the human host, substances interfering electronic transport from por might be well suited for eradication therapies against the bacterium. h. pylori flavodoxin presents a peculiar cofactor (fmn) binding site, compared to other known flavodoxi ... | 2005 | 15752617 |
| interaction of amoxicillin with dna in human lymphocytes and h. pylori-infected and non-infected gastric mucosa cells. | amoxicillin is a penicillin derivative belonging to a group of beta-lactam antibiotics used in helicobacter pylori eradication. clinical application of amoxicillin is underlined by its antibacterial activity, but little is known about its interaction with dna of human cells. using the alkaline comet assay we investigated the genotoxicity of amoxicillin in human peripheral blood lymphocytes as well as in h. pylori-infected and non-infected human gastric mucosa cells. to assess the role of reactiv ... | 2005 | 15766919 |
| long-term strict monitoring of plasma ghrelin and other serological markers of gastric diseases after helicobacter pylori eradication. | we report on a case of chronic atrophic gastritis in which the serological markers of gastric diseases were strictly monitored for 2 years after successful helicobacter pylori (h. pylori) eradication. a 31-year-old man with upper abdominal pain was diagnosed as having h. pylori infection. laboratory examination revealed low serum levels of pepsinogen (pg) i, low pg i/ii ratio, and low plasma levels of ghrelin. upper gastrointestinal endoscopy revealed severe corpus-dominant atrophic gastritis. h ... | 2005 | 15782980 |
| helicobacter pylori in patients can be killed by visible light. | helicobacter pylori colonizes the mucus layer of the human stomach and may cause peptic ulcer and adenocarcinoma. novel antimicrobial approaches are sought due to the occurrence of antibiotic resistance and consequent treatment failure. we report here that h. pylori is susceptible to inactivation by blue light. | 2005 | 15791671 |
| immune subversion by helicobacter pylori. | to maintain prolonged colonization of the human gastric mucosa, helicobacter pylori must avoid both innate and adaptive immune responses. during its long coexistence with humans, it has evolved complex strategies to maintain a mild inflammation of the gastric epithelium while limiting the extent of immune effector activity. severe disease, associated with bacterial colonization, might reflect loss of this control. several mechanisms and the bacterial factors involved in immune subversion have, i ... | 2005 | 15797510 |
| evaluation of the stool antigen test for helicobacter pylori in children and adolescents. | the stool antigen test for helicobacter pylori is a noninvasive immunoassay to diagnose active infection with helicobacter pylori in human fecal samples. its performance in children and teenagers has been tested in some developed countries, showing a sensitivity and specificity above 90%, however, its accuracy in developing countries and in children under 6 years is not well established. to determine the accuracy of the test for diagnosing helicobacter pylori infection in children and teenagers, ... | 2005 | 15810625 |
| fibrin glue, healing of gastric mucosal injury, and expression of growth factors: results from a human in vivo study. | fibrin glue is used in the endoscopic therapy of bleeding ulcerations. accelerated closure of ulcers has been attributed to this treatment; the biologic reason, however, remains unclear. | 2005 | 15812409 |
| identification of two new helicobacter pylori surface proteins involved in attachment to epithelial cell lines. | helicobacter pylori causes the development of gastritis, gastric ulcers and adenocarcinomas in humans. the establishment of infection is influenced by adherence to the gastric epithelium, and several bacterial adhesins and host cell receptors have been identified. h. pylori recognize the lewis(b) receptor through the baba adhesin but also readily adhere to epithelia in the absence of the lewis(b) epitope, demonstrating the relevance of additional adhesive interactions. this study presents a nove ... | 2005 | 15824418 |
| difficulties in assessing cytomegalovirus-associated gastric perforation in an hiv-infected patient. | active cytomegalovirus (cmv) infection is a common complication in advanced symptomatic human immunodeficiency virus (hiv) infection. cmv-induced intestinal perforations are hard to diagnose and may be observed throughout the gastrointestinal tract. isolated stomach perforation is exceptional. | 2005 | 15829006 |
| helicobacter pylori and other helicobacter species in gallbladder and liver of patients with chronic cholecystitis detected by immunological and molecular methods. | despite several recent reports on the detection of helicobacter dna in human bile, there are still uncertainties concerning the correlation of these findings with biliary tract and liver diseases. | 2005 | 15841721 |
| primerselect: a transcriptome-wide oligonucleotide primer pair design program for kinetic rt-pcr-based transcript profiling. | we describe primerselect, a program capable of transcriptome-wide design of primer pairs for optimal performance in kinetic reverse-transcriptase polymerase chain reaction (rt-pcr). for the yeast saccharomyces cerevisiae, primerselect designs primer pairs for 86% of genomic open reading frames (orfs) using design criteria we previously established to be optimal for kinetic rt-pcr (krt-pcr)-based transcript quantitation. primer pairs designed by primerselect for 230 yeast orfs were evaluated for ... | 2005 | 15865983 |
| macrolide-affected toll-like receptor 4 expression from helicobacter pylori-infected monocytes does not modify interleukin-8 production. | macrolide antibiotics have an anti-inflammatory effect by suppressing lipopolysaccharide-induced il-8 production. il-8 secretion from monocytes is observed in helicobacter pylori infection. although cag gene products are known to induce il-8 secretion, whether other bacterial substances can initiate the reaction is not determined. in this study, we show that clarithromycin induced down-regulation of toll-like receptor 4 expression and did not lead to a decrease in il-8 production and h. pylori l ... | 2005 | 15866212 |
| restriction of dna encoding selectable markers decreases the transformation efficiency of helicobacter pylori. | helicobacter pylori populations recovered from the human stomach display extensive recombination and quasispecies development, and this suggests frequent exchange of dna between different strains in vivo. in vitro, however, most h. pylori strains display restriction to the uptake of non-self dna, as measured using selectable markers, regardless of their competency for transformation with self dna. we have examined the effect of different selectable markers on double-crossover recombination effic ... | 2005 | 15866218 |
| involvement of proinflammatory cytokines il-1beta and il-6 in progression of human gastric carcinoma. | gastric carcinoma occurs in response to chronic inflammation of gastric mucosa infected with helicobacter pylori. it is not known how cytokines affect the growth and progression of gastric carcinoma. | 2005 | 15868900 |
| theodore e. woodward award: global warming and the human stomach: microecology follows macroecology. | just as there have been 20th century changes in our "macroecology," including global warming, there have been alterations in our "microecology," involving the microbial populations that colonize the human body. helicobacterpylori, an ancient inhabitant of the human stomach, has been disappearing over the course of the 20th century. as such, by comparing h. pylori+ and h. pylori- persons, the consequences of its colonization can be determined. the presence of h. pylori is associated with increase ... | 2005 | 16555606 |
| environment as a critical factor for the pathogenesis and outcome of gastrointestinal disease: experimental and human inflammatory bowel disease and helicobacter-induced gastritis. | environmental factors play an important role in the manifestation, course, and prognosis of diseases of the gastrointestinal tract such as inflammatory bowel disease (ibd) and helicobacter pylori-induced gastritis. these two disease complexes were chosen for a discussion of the contribution of environmental factors to the disease outcome in humans and animal models. dissecting complex diseases like ibd and helicobacter-induced gastritis has shown that the outcome of disease depends on the alleli ... | 2005 | 16582581 |
| predicting protein-protein interactions using signature products. | proteome-wide prediction of protein-protein interaction is a difficult and important problem in biology. although there have been recent advances in both experimental and computational methods for predicting protein-protein interactions, we are only beginning to see a confluence of these techniques. in this paper, we describe a very general, high-throughput method for predicting protein-protein interactions. our method combines a sequence-based description of proteins with experimental informati ... | 2005 | 15319262 |
| helicobacter pylori infection causes persistent platelet activation in vivo through enhanced lipid peroxidation. | we aimed at investigating the relationship between helicobacter pylori infection and in vivo lipid peroxidation and platelet activation, as reflected by urinary 8-iso-prostaglandin (pg)f(2alpha) and 11-dehydro-thromboxane (tx)b2, respectively, in otherwise healthy dyspeptic subjects. | 2005 | 15472127 |
| epidemiology of non-hodgkin's lymphoma (nhl): trends, geographic distribution, and etiology. | while for most cancers incidence and mortality are decreasing, those of non-hodgkin's lymphoma (nhl) are steadily increasing. research to define reasons for this increase is extensive, but has not yet resolved them. we have conducted a literature analysis on trends regarding changes in the incidence, geographic distribution, and etiologic factors of nhl. from our own and previous analyses, an increasing nhl incidence at a rate of 3-4% per year was observed for the 1970s and 1980s. this stabilize ... | 2005 | 15480663 |
| specific immunoglobulin a antibodies in maternal milk and delayed helicobacter pylori colonization in gambian infants. | immunoglobulin a (iga) in maternal milk may protect gambian infants from early helicobacter pylori colonization. this study sought evidence that this protection could be due to specific iga antibodies. | 2004 | 15486839 |
| nod1 responds to peptidoglycan delivered by the helicobacter pylori cag pathogenicity island. | epithelial cells can respond to conserved bacterial products that are internalized after either bacterial invasion or liposome treatment of cells. we report here that the noninvasive gram-negative pathogen helicobacter pylori was recognized by epithelial cells via nod1, an intracellular pathogen-recognition molecule with specificity for gram-negative peptidoglycan. nod1 detection of h. pylori depended on the delivery of peptidoglycan to host cells by a bacterial type iv secretion system, encoded ... | 2004 | 15489856 |
| [genetic susceptibility and risk of gastric cancer in a human population of cauca, colombia]. | gastric cancer (gc) is the main cause of mortality by cancer in colombia. glutathione s-transferase (gst) enzymes are involved in the detoxification of many environmental carcinogens. the homozygous deletions of glutathione s-transferase m1 (gstm1-0) and glutathione s-transferase t1 (gstt1-0) have been associated with several types of cancer. the risk to develop gc has been associated with environmental factors and helicobacter pylori infection. the tumor necrosis factor (tnf-alpha) and its leve ... | 2004 | 15495595 |
| review article: relationship between the metabolism and efficacy of proton pump inhibitors--focus on rabeprazole. | proton pump inhibitors are now considered the mainstay of treatment for acid-related disease. although all proton pump inhibitors are highly effective, the antisecretory effects of different drugs in this class are not completely consistent across patients. one reason for this is the acid-suppressing effect of helicobacter pylori infection, which may augment the actions of proton pump inhibitors. a second important reason for interpatient variability of the effects of proton pump inhibitors on a ... | 2004 | 15496214 |
| pathogen burden, inflammation, proliferation and apoptosis in human in-stent restenosis. tissue characteristics compared to primary atherosclerosis. | pathogenic events leading to in-stent restenosis (isr) are still incompletely understood. among others, inflammation, immune reactions, deregulated cell death and growth have been suggested. therefore, atherectomy probes from 21 patients with symptomatic isr were analyzed by immunohistochemistry for pathogen burden and compared to primary target lesions from 20 stable angina patients. while cytomegalovirus, herpes simplex virus, epstein-barr virus and helicobacter pylori were not found in isr, a ... | 2004 | 15528935 |
| [probiotics: from metchnikoff to the current preventive and therapeutic possibilities]. | about a century ago, metchnikoff first hypothesised that some intestinal bacteria "produce compounds useful against a premature ageing". since then, studies progressed over last century, leading to a remarkable improvement of the knowledge about the role of intestinal micro-organisms. nowadays a number of different micro-organisms satisfying certain requisites are named probiotics and are produced on a large scale. at present, a rational use of probiotics with preventive and therapeutic purposes ... | 2004 | 15529809 |
| helicobacter pylori-induced epithelial cell signalling in gastric carcinogenesis. | helicobacter pylori represents a highly successful human microbial pathogen that infects the stomach of more than half of the world's population. h. pylori induces gastric inflammation, and the diseases that can follow such infection include chronic gastritis, peptic ulcers and, more rarely, gastric cancer. the reasons why a minority of patients with h. pylori develops gastric cancer could be related to differences in host susceptibility, environmental factors and the genetic diversity of the or ... | 2004 | 14700549 |
| analysis of geospecific markers for helicobacter pylori variants in patients from japan and nigeria by triple-locus nucleotide sequence typing. | human migrations and geographical separation over long periods may have resulted in ecologically distinct populations of helicobacter pylori infecting individuals in different continents. this study used nucleotide sequence analysis with the aim of defining population-specific genomic motifs in isolates from east asian and african dyspeptic patients. sequences of internal fragments (542-627 bp) of three housekeeping genes (urei, ahpc and atpa) were analysed for 85 isolates from individuals in ja ... | 2004 | 14702408 |
| infiltration of cd8+ t cells containing rantes/ccl5+ cytoplasmic granules in actively inflammatory lesions of human chronic gastritis. | chronic gastritis is frequently associated with infection of helicobacter pylori and characterized by tissue infiltration of neutrophils, lymphocytes, and plasma cells. to address the mechanism of lymphocyte infiltration in chronic gastritis, we examined the expression of chemokines and their receptors using frozen sections of chronic gastritis, obtained from 23 patients who underwent gastrectomy for gastric cancer. by immunohistochemistry, lymphocytes in inflamed gastric mucosa expressed ccr5 a ... | 2004 | 14704722 |
| oxidative-stress-related proteome changes in helicobacter pylori-infected human gastric mucosa. | helicobacter pylori infection leads to gastroduodenal inflammation, peptic ulceration and gastric carcinoma. proteomic analysis of the human gastric mucosa from the patients with erosive gastritis, peptic ulcer or gastric cancer, which were either infected or not with h. pylori, was used to determine the differentially expressed proteins by h. pylori in the human gastric mucosa in order to investigate the pathogenic mechanism of h. pylori -induced gastric diseases. prior to the experiment, the e ... | 2004 | 14711373 |
| helicobacter pylori and gastroesophageal reflux disease. | since the rediscovery of helicobacter pylori two decades ago, it has become increasingly clear that the true relationships between this organism and diseases of the upper gastrointestinal tract are highly complex. h. pylori colonization is a strong risk factor for peptic ulceration and distal gastric cancer; however, gastritis has no adverse consequences for most hosts, and the prevalence of h. pylori is inversely related to gastroesophageal reflux disease (gerd) and its sequelae, which include ... | 2004 | 14723839 |
| is the host responsible? -- the role of the immune system in helicobacter pylori infection. | helicobacter pylori is the primary aetiological agent of chronic gastritis and a necessary factor in most peptic ulcer disease. to date the majority of studies, which have examined factors predictive of disease outcome, have concentrated on bacterial virulence factors. recent evidence suggests that the immune response might be responsible. fc receptors polymorphisms have been implicated in autoimmune diseases and persistent bacterial infections. individuals who are h. pylori positive and homozyg ... | 2004 | 14729011 |
| extracellular signal-regulated protein kinase mediates interleukin 17 (il-17)-induced il-8 secretion in helicobacter pylori-infected human gastric epithelial cells. | helicobacter pylori-induced mucosal inflammation results in high production of interleukin 17 (il-17), a potent inducer of il-8 in gastric epithelial cells. the aim of this study was to investigate signaling pathways by which il-17 regulates il-8 production in human gastric epithelial cells. activation of mitogen-activated protein (map) kinases in both il-17-stimulated mkn28 cells and epithelial cells isolated from h. pylori-colonized gastric mucosa was assessed by western blotting. in il-17-sti ... | 2004 | 15321994 |
| differential expression of bax and bcl-2 in gastric mucosa. | 2004 | 15326840 | |
| one patient, two lymphomas. simultaneous primary gastric marginal zone lymphoma and primary duodenal follicular lymphoma. | we report the case of a 73-year-old man who presented with a 2- to 3-month history of epigastric discomfort and guaiac-positive stool. an upper gastrointestinal endoscopy revealed a diffuse erythematous nodular mucosa and submucosal thickening in the stomach. diffuse mucosal nodularity was also found in the second portion of the duodenum. a complete workup with histologic, immunohistochemical, and molecular studies revealed 2 distinct, apparently unrelated lymphomas, namely, a gastric marginal z ... | 2004 | 15335250 |
| receptor-mediated targeting of lipobeads bearing acetohydroxamic acid for eradication of helicobacter pylori. | in the present context, phosphatidyl ethanolamine (pe) liposomes anchored polyvinyl alcohol (pva) xerogel beads (lipobeads) bearing acetohydroxamic acid (aha) was developed as a receptor-mediated drug delivery system for use in blocking adhesion of helicobacter pylori and thereby preventing the sequelae of chronic gastric infections. pva beads containing aha were prepared by emulsification followed by low temperature crystallization method. surface acylation with fatty acid chain was accomplishe ... | 2004 | 15342178 |
| [pathogenesis and treatment of refractory gastroesophageal reflux disease in japanese patients]. | prevalence of refractory gastroesophageal reflux disease (gerd) defined as a patient who have persistent gerd symptoms during treatment with proton pump inhibitor (ppi) is rare in japanese patinets. pathogenesis of refractory gerd is associated with several factors including dysfunction of esophageal motility, presence of severe hiatal hernia, complication such as stricture and short esophagus, extensive metabolizer of cyp2c19 genotype, nocturnal gastric acid breakthrough, absence of h. pylori i ... | 2004 | 15344542 |
| effect of h. pylori on the expression of trail, fasl and their receptor subtypes in human gastric epithelial cells and their role in apoptosis. | in the human stomach expression of tnf-related apoptosis inducing ligand (trail) and its receptors and the modulatory role of helicobacter pylori are not well described. therefore, we investigated the effect of h. pylori on the expression of trail, fasl and their receptors (trail-r1-r4, fas) in gastric epithelial cells and examined their role in apoptosis. | 2004 | 15361075 |