Publications

skin testing, fecal culture, and lymphocyte immunostimulation in cattle inoculated with mycobacterium paratuberculosis.	fourteen calves at 21 days of age were experimentally inoculated with 100 mg (wet weight) of mycobacterium paratuberculosis. three calves were inoculated orally, 4 intravenously, and 7 subcutaneously. lymphocyte immunostimulation, fecal culture, and intradermal tuberculin skin testing were done between 112 to 150 days following exposure. lymphocyte immunostimulation test results, conducted at 112 days after inoculation, showed all animals positive to mycobacterium avium purified protein derivati ...	1977	339793
in vitro stimulation of bovine peripheral blood lymphocytes: suppression of phytomitogen and specific antigen lymphocyte responses by aflatoxin.	the effects of aflatoxin b1 on responses of the peripheral blood lymphocytes (pbl) of 2 normal animals to phytohemagglutinin, concanavalin-a, and pokeweed mitogen and of the pbl of 2 mycobacterium bovis-infected animals to phytohemagglutinin and purified protein derivative of m bovis (ppd) were studied. aflatoxin concentrations of greater than or equal to 10 microgram/ml significantly suppressed the lymphocyte response of normal animals to the phytomitogens. lymphocyte response of m bovis-infect ...	1977	339794
[preferential differentiation of hematopoietic stem cells transferred to mice previously infected by bcg].	following injection of bone marrow cells in lethally irradiated mice, previously infected with bcg regenerating hemopoietic cell populations differentiate along the leucocyte pathway to the detriment of erythroid lineage. such a phenomenon persisting even if anemia of infected mice is increased by bleeding just before irradiation and reconstitution supports the hypothesis of preferential differentiation of stem cells.	1977	406087
flow microfluorometric quantitation of the blastogenic response of lymphocytes.	a method for quantitating the specific stimulation of peripheral lymphocytes has been developed using the techniques of flow microfluorometry. peripheral bovine lymphocytes were collected and specifically stained for deoxyribonucleic acid (dna) content using a low-salt propidium iodide procedure. flow microfluorometry was used to determine, on the basis of dna content, the percentage of cells in a population that was stimulated. extremely uniform staining of the lymphocytes (coefficient of varia ...	1976	768372
effect of systemic bcg infection in syrian golden hamsters.	the response of syrian golden hamsters to systemic infection with several doses of mycobacterium bovis (strain bcg) was assessed. large numbers of organisms (10(7)), injected intravenously, were lethal for hamsters, whereas all animals survived infection with 10(4) colony-forming units of bcg. animals responded immunologically to purified protein derivative as assessed by increased footpad swelling and splenic lymphocyte proliferation. the immediate cause of death was a diffuse granulomatous int ...	1976	780276
adjuvant activity of mycobacterial fractions. i. purification and in vivo adjuvant activity of cell wall skeletons of mycobacterium bovis bcg, nocardia asteroides 131 and corynebacterium diphtheriae pw8.	the adjuvant activity of cell wall skeletons (mycolic acid-arabino-galactan-mucopeptide, cws) prepared from the cells of mycobacteria, nocardia and corynebacteria was examined in vivo in mice and guinea pigs. the cell wall skeletons of mycobacterium bovis bcg (bcg-cws), nocardia asteroides 131 and corynebacterium diphtheriae pwc suspended in freund's incomplete adjuvant (fia) as water-in-oil emulsions showed potent adjuvant activity on the formation of circulating antibody and cell-mediated immu ...	1975	807194
comparison of the specificity of human and bovine tuberculin ppd for testing cattle. 2. south-eastern england.	a tuberculin testing trial was carried out in eight counties of south-eastern england to compare the specificity for bovine tuberculosis of weybridge human ppd with that of rotterdam bovine ppd. the matching of these two tuberculins for potency in naturally infected cattle had already been established, the bovine ppd being approximately one-and-a-half times more potent than the human ppd per unit of weight. in 1110 cattle in 25 herds with histories of long-standing freedom from tuberculosis and ...	1975	47751
evaluation of a comparative intradermal tuberculin test in california dairy cattle.		1974	4594742
the diagnosis of tuberculosis in cattle with special reference to bovine ppd tuberculin.		1973	4197735
thermography of tuberculin reactions in cattle.	the allergic reactions of dermal induration and hyperthermia were compared in tuberculin-hypersensitive calves. tuberculins prepared from mycobacterium avium, m. bovis, and m. paratuberculosis were administered intradermally and the responses were compared. the indurations from immediate and delayed hypersensitivity reactions were readily distinguishable, but the hyperthermic responses appeared to contain elements of both immediate and delayed hypersensitivity.	1973	4202962
[pathomorphology of intestinal tuberculosis in cows].		1972	4579996
antibody formation to trehalose-6,6'-dimycolate (cord factor) of mycobacterium tuberculosis.	a complex of trehalose-6, 6'-dimycolate (cord factor) and methylated bovine serum albumin was prepared, and its effect on the toxicity of cord factor in mice was studied. either the active immunization with cord factor-methylated bovine serum albumin complex or the passive transfer of sera of rabbits vaccinated with the complex protected mice against the toxic action of cord factor. the antisera of mice and rabbits and gamma globulin fraction of rabbit antiserum demonstrated a precipitin reactio ...	1972	4117880
employment of tuberculostasis in serum-agar medium for the study of production and activity of mycobactin.	mycobactin (m), an iron-chelating product of tubercle bacilli, neutralized serum tuberculostasis by removing growth-essential iron from transferrin (tr) and supplying the metal to the bacteria. the competition for iron between tr and m has been demonstrated by the agar-plate diffusion test. this test is suitable not only for the study of tr-iron-m interplay but also for the evaluation of serum tuberculostasis. extremely poor solubility of m in water and consequently its association with lipoidal ...	1971	5005292
the microstructure of colonies of the connaught bcg strain.	it has previously been shown that there is a certain correlation between the biological properties of bcg strains-properties on which their immunogenicity and allergenicity depend-and the macroscopic appearance of the growth of these colonies on solid and liquid media. to investigate this phenomenon in greater detail, the author examined the microstructure of colonies of the connaught bcg strain grown on both solid and liquid media. colonies were fixed in agar, embedded in paraffin, sectioned an ...	1970	4925828
some recent results in isolating atypical mycobacteria from cattle and their environment.		1967	4964966
glycoprotein levels in cattle naturally and experimentally infected with mycobacterium bovis.		1965	4286769
studies on differentiation of tubercle bacilli isolated from human beings, cattle and poultry.i. morphologic and cultural characteristics.		1963	14066016