Publications

cytotoxic activity of a cell-free extract of entamoeba histolytica.		1978	205017
a new technique of heterogenous enzyme-linked immunosorbent assay, stick-elisa. ii. application of stick-elisa to antigens of various infective agents.	stick-enzyme-linked immunosorbent assay (stick-elisa) was applied to antigens of a variety of infective agents. an acceptable reproducibility in a series was reached, the variation coefficient being twelve percent. preliminary results revealed that ready coated sticks could be stored at 4 degrees c for at least two to three weeks.	1978	205062
amebic peritonitis following rupture of an amebic liver abscess. successful treatment of two patients.	we present two successfully treated cases of amebic peritonitis. acute peritonitis secondary to intra-abdominal rupture of an amebic liver abscess is an infrequent but serious complication of invasive amebiasis. its diagnosis should be considered in anyone with a suspected liver abscess, jaundice, or diarrhea in whom peritonitis develops. this diagnosis should be further suggested in the united states if the patient is a male and is of mexican origin in areas where this racial group constitutes ...	1978	205190
[factors involved in the spread of enteroparasites. iii. distribution of various enteroparasitosis in 2 population groups in the city of ribeirão preto, são paulo, brazil].		1978	206950
haematogenous amoebic lung abscess. a case report.	left upper lobe amoebic lung abscess without established hepatic amoebiasis in a young black man is described and the pathogenesis is briefly discussed.	1978	206979
electrophoretic isoenzyme patterns of entamoeba histolytica and entamoeba coli.	cultures of 14 stocks of entamoeba histolytica and one only of entamoeba coli were compared by electrophoretic patterns of three enzymes: glucose-phosphate isomerase, phosphoglucomutase and l-malate: nadp+ oxidoreductase (oxaloacetate-decarboxylating). easily distinguished patterns divided e. histolytica into three groups, whilst a distinctly different pattern for e. coli was also seen.	1978	206991
a simple technique for preparing clone cultures of entamoeba histolytica.		1978	206992
entamoeba histolytica and homo sapiens.	blind acceptance of the dicta of the great has led to much confusion as to the relationship between amoeba and man. a review of the mistakes of the past may lead to a better appreciation of the present, and higher hopes for the future. an hypothesis is presented.	1978	207506
entamoeba histolytica, e. invadens, and e. moshkovskii: fluctuations of the dna content of axenic trophozoites.		1978	207550
entamoeba histolytica: uptake of purine bases and nucleosides during axenic growth.		1978	210037
modified stuart medium--a transport and maintenance medium for entamoeba histolytica.		1978	210120
pathogenicity of entamoeba histolytica--effect of cholesterol on the virulence of strains of amoebae.		1978	210121
vaccination against hepatic amebiasis in hamsters.		1978	210268
indirect fluorescent antibody test in amoebiasis using axenic entamoeba histolytica.		1978	211081
growth pattern of axenic entamoeba histolytica in different sera and the antiamoebic activity of specific antisera.		1978	211082
experimental muscular amebiasis in hamsters as a biological model.	trophozoites of entamoeba histolytica maintained in vitro in pavlova's medium were inoculated by deep intramuscular injection into the proximal left hindleg of hamsters. thioglycollate medium was utilized as a successful vehicle to induce the infection. the invasion of the muscular tissue by the vegetative forms caused the formation of abscesses with great destruction of muscular fibers. the lesions were limited to the muscular tissue of the femoral area. the number of trophozoites, the medium o ...	1978	211747
[ultramicroscopic structure of the cyst wall of entamoeba invadens, e. histolytica and e. coli].	the ultrastructure of the wall of e. invadens cysts induced in axenic cultures was compared by transmission electron microscopy of thin sections, with that of e. histolytica and e. coli cysts obtained from feces of human asymptomatic carriers. the cyst wall of the three species studied had similar ultrastructural features; in all, the wall was composed mainly by the aggregation of filaments adjacent to the plasma membrane. the cytoplasmic components which might be involved in the synthesis and d ...	1978	211940
[instability of the dna content in entamoeba histolytica].	dna content was repeatedly determined during a 10-month period on axenically cultured tropho-zoites from three strains (hk-9:nih, hm-2:imss and hm-3:imss) and a clone of hk-9:nih strain of entamoeba histolytica, by means of the diphenylamine reaction. average dna content was more or less variable in all of them. according to their extent, variations were: 1) large (almost four times in strain hm-2:imss), or 2) small (1.5 times in the clonal isolate). with this relatively small sample, three stat ...	1978	211941
isolation and sedimentation analysis of rna from entamoeba histolytica.		1978	211942
[amino acid composition in total proteins of strains of entamoeba histolytica in axenic cultures].	in this paper we detail the amino acid composition of total protein hydrolyzates from trophozoites of two entamoeba histolytica strains (hk-9:nih and hm-2:imss) cultured axenically in tp-s-1 medium. since we obtained nearly identical results in both strains, amino acid patterns may constitute an additional taxonomic criterion of the species. our data are strikingly different from those found previously by other workers with total proteins from hk-9:nih trophozoites cultured in a bacteria-conditi ...	1978	211943
uptake of selected pyrimidines by axenically grown entamoeba histolytica.	this study was conducted to determine the mode(s) of uptake of selected pyrimidine bases and nucleosides by axenically grown entamoeba histolytica. two-minute uptake studies with 3h-labeled compounds showed the following: 1) uridine and cytidine are taken up, in part, by a carrier mediated system; 2) uracil, cytosine, thymine and thymidine enter amebae via passive diffusion; both cytidine and uridine are taken up by passive diffusion when present at exogenous concentration greater than 500 mum; ...	1978	211944
[immunochemistry of immunogenic proteins of entamoeba histolytica].	entamoeba histolytica (hk-9: imss) trophozoites were separated from the culture medium and washed and submitted to the effects of ultrasound during 10 minutes. after 10,000 rpm x 30 centrifugation, the supernatant fluid was separated in four fractions by sephadex g-200 gel chromatography. these fractions were lyophilized, redisolved in a lesser volume and dyalized against isotonic saline solution. antigen-antibody precipitant reactions between each of them and two rabbit antiserums were demonstr ...	1978	211945
[cytopathogenic activity of extracts e. histolytica trophozoites].	previous studies have not shown any correlation between enzyme activities and pathogenicity of entamoeba histolytica. the activities analyzed were selected without having proved their involvement in the cytopathogenicity. we are using the reverse approach to study the cytopathogenic factors, i.e., to detect and characterize first the cytopathic effect of amebic extracts on animal cells, and subsequently to try to identify the components involved and to explore their correlation with amebic virul ...	1978	211946
[effects of nordihydroguairetic acid and ethanol on the growth of entamoeba invadens].	the effects of the alcoholic extract (resin) obtained from larrea tridentata, of nordihydroguiaretic acid (ndga) and of ethanol over axenic cultures of entamoeba invadens pz strain were studied. the activity of these substances was demonstrated in growht curves. inocula of 1-3 x 10(5) trophozites by milliliter in culture tubes containing 11 ml of tp-s-1 medium were done, and incubated at 25 degree c. in each experiment the follwoing was done: a) tipical growth curve as a control, b) curve with t ...	1978	211947
amebal viruses and the virulence of entamoeba histolytica.		1978	211948
variable toxicity within the hk 9 strain of entamoeba histolytica.		1978	211949
surface ultrastructure of entamoeba histolytica; a study by high voltage transmission electron microscopy (hvtem) and scanning electron microscopy (sem).		1978	211950
the development and standaridization of an elisa method for the detection of entamoeba histolytica antigens in fecal samples.	recognizing the difficluties associated with the microscopic detection and identification of entamoeba histolytica in fecal samples, an immunochemical method of detection of e. histolytica antigens in fe-es has been developed. for a number of reasons, e.g., the known fragility of e. histolytica trophozoites, it was felt that intestinal amebiasis would be associated with detectable levels of e. histolytica anti-ens in feces from infected patients. a technique for harvesting amebic antigens in fec ...	1978	211951
[identification of entamoeba histolytica membrane antigens with antibodies of amebiasis patients].	the specificity of sera from patients with amoebiasis was assayed on trophozoites of e. histolytica hk9, hm15 and hm2 cultured axenically and monoxenically. glutaraldehyde fixed cell were incubated with samples of serum, the excess of igs was washed out and the presence of unbound material of specifically bound antibodies was measured with radiolabeled protein a from staphylococcus aureus. a positive reaction with hk9 was observed in 18 per cent of the 66 sera tested. an immune crossreaction res ...	1978	211952
[phagocytosis of human erythrocytes by entamoeba histolytica. quantitative study].	the degree of eythrophagocytosis of two recently isolated strains of e. histolytica was measured by microscopic examination. amebas isolated from a patient with amebic rectocolitis (strain hm22:imss, monoxenic) ingested human red blood cells faster and in larger numbers than trophozoites isolated from an asymptomatic carrier (hm27:imss, monoxenic). the results suggest that an increased rate of phagocytosis could be one of the surface properties characteristic of the invasive strains of e. histol ...	1978	211953
production of cecal lesions in newborn guinea pigs with axenically cultivated entamoeba histolytica.	the production of cecal lesions with axenically cultivated entamoeba histolytica in an experimental animal is reported for the first time. forty-three newborn guinea pigs (one to five days old) were inoculated via laparatomy with 2.5 x 10(5) to 2 x 10(6) strain hm-1-imss amebae. of 23 animals surviving to the 7th postinoculation day all showed cecal lesions at necropsy regardless of the age of the animal when used or the number of amebae received. ten animals had lesions visible to the unaided e ...	1978	211954
[culture and establishment of new mexican strains of entamoeba histolytica].	mixed and monoxenic cultures of rectal exudates from three patients with amebiasis of colon, and of feces from three asymptomatic carriers of entamoeba histolytica obtained after administration of a saline cathartic, and from a case of exudate of the perianal cutaneous lesion border produced by this parasite, were done. the culture media used were for: mixed culture, boeck and drbohlav modified culture and, for monoxenic culture, diamond's monophasic tp-s-1 medium without vitamin mixture and usi ...	1978	211955
cytotoxicity of a cell free extract of entamoeba histolytica.	previous attempts to demonstrate toxicity of cell-free extracts prepared from axenically cultivated e. histolytica have proved unsuccessful. we report here the successful production of cytopathic effect by cell-free extracts of two strains (hm-1 and hk-9) of e. histolytica in tissue culture monolayers. the cytotoxic effect demonstrated was sensitive to serum in the assay medium. failure of past investigations to demonstrate cytotoxic activity may be explained by the presence of serum in the assa ...	1978	211956
clonal growth of entamoeba in agar: some applications of this technique to the study of their cell biology.	a new technique for growing single entamoeba trophozoites into colonies in agar has been developed. this method depended upon axenic cultivation and utilized diamond's new tyi-s-33 medium. the present paper describes several different types of experiment which have utilized the agar technique. a) cloning. the isolation of clones from agar way easy and successful. clones of the hm-1 strain did not differ in virulence for newborn hamster liver or in colony morphology or colony forming efficiency. ...	1978	211957
restoration of virulence of axenically-cultivated entamoeba histolytica by cholesterol.	the lost pathogenicity of two strains of entamoeba histolytica, one isolated in 1924 and the other in 1967, grown in axenic culture for the past five and six years respectively, was restored by supplementing the culture medium with cholesterol through a number of transfers. the number of passages in the cholesterol supplemented medium, necessary to restore a certain degree of pathogenicity of the two strains in hamsters, was proportional to the total time of in vitro cultivation of the strain, a ...	1978	211958
[an in vitro model for the quantitative study of e. histolytica virulence].	an experimental model for the quantitative study of the cytopathic effect of e. histolytica is described. confluent cultures of the mdck cell line, which form a monolayer with morphological and electrophysiological features of an epithelium were used. trophozoites of the strains hm1:imss, hm2:imss and hm3:imss, axenic produced variable degrees of cytopathic effects of mdck cultures each one characteristic for a given strain. the degree of damage induced by the different strains of amebas was mea ...	1978	211959
[interaction between entamoeba histolytica and the cecal epithelium in the guinea pig].	the early interaction between e. histolytica trophozoites and the intestinal mucosa was studied by light and electron microscopy using sheets of isolated epithelium of the guinea pig colonic mucosa. the integrity of the isolated epithelium was confirmed by scanning electron microscopy. after 30 minutes of interaction, the amebas adhered first, and penetrated subsecuently the epithelium of the colon at the interglandular regions. the results suggest that the trophozoites of e. histolytica invade ...	1978	211960
[ultramicroscopic study of the invasion of the mucosa of the colon by entamoeba histolytica in axenic cultures].	animal models have been utilized to study experimentally produced intestinal amebiasis. the guinea pig has been the animal of choice to study the mechanisms by which trophozoites of e. histolytica produce colonic lesions. the purpose of this study was to produce lesions in the colon of newborn guinea pigs by the injection of e. histolytica, hm-1 imss, strain grown axenically, and to characterize the early changes with the light and electron microscopes. a total of 35 guinea pigs, one to two days ...	1978	211961
[serology with polysaccharides of entamoeba histolytica].	the immunochemical activity of a polysaccharidic antigen derivated from e. histolytica was studied. this polysaccharide was tested by means of counterimmunoelectrophoresis (cie) and radial immunodiffusion (idr) against sera from patients with diagnosis of intestinal and hepatic amebiasis. in order to perform a test most significative to the problem sera, these were submitted previously to passive hemagglutination, cie and idr with total ameba antigen. in hepatic amebiasis it can be observed that ...	1978	211962
[activity of the alternative complement pathway by various axenic strains of entamoeba histolytica].	the activation of the alternative pathway of complement by axenic cultures of e. histolytica is reported. the activation is carried out in different proportions by several axenic strains, i.e., hk9:nih, hm1:imss, hm2:imss and hm3:imss; the end result consists of a destruction of the parasite as well as consumption of the serum complement. the participation of the alternative pathway was studied in the presence of egta and mg++, zymosan, heat-inactivation at 50 degree c for 20 min and immunoelect ...	1978	211963
[immunite reaction of hamsters infected or immunized with entamoeba histolytica].	the immune response in hamsters either infected or immunized with entamoeba histolytica was studied. the response was tested by determination of antibodies by the counterimmunoelectrophoresis technique and by lymphocyte transformation induced by the amebic antigen. the results obtained showed differences among both groups when the ability of amebic antigen to induce 3h-thymidine incorporation of lymphocyte cultures is compared. however, the response of lymphocyte cultures to concanavalin a was s ...	1978	211964
[induction of protective anti-ameba immunity with new antigens in the suckling hamster. a. introduction].		1978	211965
[induction of protective anti-ameba immunity with new antigens in the suckling hamster. b. antigenic material].		1978	211966
entamoeba histolytica: iron and nutritional immunity.		1978	211967
[determination of an anti-ameba antigen in the feces by the elisa method (enzyme-linked immunosorbent assay) for the identification of entamoeba histolytica].		1978	211968
[a new hemagglutination method to determine antibodies against entamoeba histolytica].	a passive hemagglutination method useful for the determination of antibodies against entamoeba histolytica is described. the e. histolytica antigen is attached to group "o" human red blood cells by means of crcl3. the specificity and sensitivity of the crcl3 method was compared with other hemagglutination methods, i.e., tannic acid method and a commercial preparation, as well as counterimmunoelectrophoresis. the crcl3 method showed a reactivity similar to the tannic acid hemagglutination techniq ...	1978	211969
achievements and obstacles in research for new anti-amoebic drugs.		1978	211970
inorganic pyrophosphatase and nucleoside diphosphatase in the parasitic protozoon, entamoeba histolytica.		1978	212030
biomedical survey in the moluccas, indonesia.		1978	212835
serial measurement of total and parasite-specific ige in an african population infected with entamoeba histolytica.		1978	212850
a new medium for the axenic cultivation of entamoeba histolytica and other entamoeba.		1978	212851
[invasion of the mucosa of the colon by entamoeba histolytica. recent observations].		1978	213335
[technics of measuring the motive force of very small ameboid cells].	a technic have been perfected which measures directly the motive force of very small ameboid cells and indicates the tropism of the amoeba. by using this technic, the motive force of entamoeba histolytica was measured. it varied between +/- 1.5 x 10-4 dyne. we have also shown chimiotactism in entamoeba histolytica. the metabolic coumpound excreted by this cell induced negative tropism.	1978	213820
experimental amebiasis. iii. a rapid in vitro assay for virulence of entamoeba histolytica.	a rapid and simple assay for cytopathogenicity of axenically cultivated entamoeba histolytica has been developed employing baby hamster kidney (bhk) or mouse 3t3 cells conventionally tissue cultured. three of the twelve amebal strains tested produced total destruction of the bhk cell monolayer in 2--3 hours, and these three strains are the three most virulent strains for the newborn hamster liver. two additional strains were of moderate cytopathogenicity in vitro and of moderate virulence in viv ...	1978	213981
hepatic amebiasis in spider monkeys.	in the past, investigators have been able to produce hepatic amebiasis in laboratory animals only by direct introduction of parasites into the liver or its vasculature, or by other artificial manipulations. a natural model of human visceral amebiasis has been lacking. we document an extensive outbreak of amebic dystentery which took place in a colony of spider monkeys; severe hepatic abscesses occurred in many animals. the spider monkey is highly susceptible to infection with entamoeba histolyti ...	1978	213982
the presence of liver auto-antibodies induced by entamoeba histolytica in the sera from both naturally infected humans and immunized rabbits.	auto-antibodies against normal human liver have been detected in the sera of humans with highly positive indirect hemagglutination (iha) amebiasis titers and with clinically-proven amebic liver abscess. sera of amebiasis patients and rabbits immunized with killed entamoeba histolytica were tested for anti-amebic antibodies by the iha test and for auto-antibodies by the complement fixation test, using the antigens prepared from extracts of human liver and rabbit liver. a direct correlation was fo ...	1978	213983
immunologic studies on hamsters infected with entamoeba histolytica.	the serologic and cell-mediated immune responses of hamsters exposed to 2 strains of entamoeba histolytica (hm-1 and hm-19) were evaluated by a series of in vitro tests. the pathogenicity of the 2 strains was evaluated in terms of their ability to produce liver abscesses and spleen enlargement. antibody response was evaluated by the indirect hemagglutination test. the cellular immune response was assayed by increased dna synthesis by lymphocytes and migration inhibition of macrophages.	1978	214539
susceptibility of various strains of mice to entamoeba histolytica.		1978	214540
[imported protozoan and worm infections].		1978	214675
[effect of bacteria on the virulence of an entamoeba histolytica strain long growing in the presence of metronidazole].		1978	214693
the differentiation of invasive and non-invasive entamoeba histolytica by isoenzyme electrophoresis.	cultures of 85 stocks of entamoeba histolytica were compared by electrophoretic patterns of three enzymes: glucosephosphate isomerase, phosphoglucomutase and l-malate: nadp+ oxidoreductase (oxaloacetate-decarboxylating). four groups of e. histolytica stocks have been distinguished. 18 of these stocks were derived from individual patients with clinical amoebiasis and all of these were of one enzyme group, which did not occur among the other stocks.	1978	214906
[medical aspects of work in tropical countries].		1978	214964
protective immunity and possible autoimmune regulation in amebiasis.	hamsters were injected intradermally with axenic trophozoites of a pathogenic strain of entamoeba histolytica, and controls similarly with culture medium. localized lesions were produced in all animals injected with amebae. after the lesions healed all the animals were challenged intrahepatically with axenic amebae. extensive liver abscesses were produced in all the controls, but a small abscess in only one of the vaccinated hamsters. in the protected animals the indirect hemagglutination antibo ...	1978	100068
chemotherapeutically acitve nitro compounds. 4. 5-nitroimidazoles (part iii).	more than 100 1-methyl-5-nitroimidazoles substituted in the 2-position via an oxymethyl group were synthesized and their structure-activity relationship toward various protozoa was investigated. among the derivatives substituted with an aromatic radical there are most of the compounds which are highly effective against trichomonads; 9 preparations are superior to tinidazole and 29 are superior to metronidazole in mice infected with trichomonas fetus, and 9 compounds exhibit a better effect than ...	1978	107955
dynamics of the interaction between entamoeba histolytica and components of the immune response. i. capping and endocytosis; influence of inhibiting and accelerating factors; variation of the expression of surface antigens.	the possible implications of cell membrane dynamics in the host--parasite relationship has been studied. entamoeba histolytica rapidly redistributed and internalized antibodies and con a bound to its surface. the process was dependent on temperature, cell metabolism and changes of ph in the environment. phagocytizing amoebae displayed a higher rate of membrane perturbations, which were similarly affected by temperature, cell metabolism and ph variations. cytochalasin b partially inhibited the re ...	1978	76334
pyruvate-to-ethanol pathway in entamoeba histolytica.	the pyruvate-to-ethanol pathway in entamoeba histolytica is unusual when compared with most investigated organisms. pyruvate decarboxylase (ec 4.1.1.1), a key enzyme for ethanol production, is not found. pyruvate is converted into acetyl-coa and co2 by the enzyme pyruvate synthase (ec 1.2.7.1), which has been demonstrated previously in this parasitic amoeba. acetyl-coa is reduced to acetaldehyde and coa by the enzyme aldehyde dehydrogenase (acylating) (ec 1.2.1.10) at an enzyme activity of 9 uni ...	1978	25658
membrane bound pyrophosphatases in entamoeba histolytica.	entamoeba histolytica homogenates are capable of hydrolyzing a range of inorganic and organic pyrophosphates. two separate activities are present: an inorganic pyrophosphatase hydrolyzing inorganic pyrophosphate and linear tripolyphosphate, and a nucleoside diphosphatase hydrolyzing thiamine pyrophosphate and nucleoside diphosphates (adp, gdp and udp). the inorganic pyrophosphatase has an acid ph optimum, a relatively high km (congurent to 1 micrometer) and is markedly heat stable and lacks a me ...	1978	29580
antiamoebic activity of drugs given singly and in combination against axenically grown entamoeba histolytica.		1978	29581
biological oxidations and flavoprotein catalysis in entamoeba histolytica.		1978	29583
influence of ph on the amoebicidal activity of systemically active amoebicides against axenic entamoeba histolytica.		1978	29834
amebic balanitis.		1978	201784
current concepts in parasitology. amebiasis.		1978	201844
amoebiasis.		1978	203420
bisamidines of 2,6-diaminoanthraquinone as antiamebic agents.	a series of bisamidines of 2,6-diaminoanthraquinone was synthesized and tested against cecal and hepatic forms of entamoeba histolytica infections in rats and hamsters, respectively. a number of compounds were found to have good activity against infections in both species.	1978	203694
latex agglutination test for amoebiasis in pakistani patients with chronic 'obscure' liver enlargements.	the latex agglutination test for amoebiasis was done in 50 pakistani patients in whom a clinical diagnosis of chronic non-supparative amoebic hepatitis was made on the basis of liver enlargement associated with chronic recurrent abdominal disturbance. the serological test was negative in 32 (64%) and positive in 18 (36%). examination of stool specimens showed a higher frequency of e. histolytica in serology positive patients. the liver biopsy findings were variable and included normal histology ...	1978	204801
[pathogenesis of amebiasis. 1. clinical aspects of amebiasis in brazil. study of 3 groups of population of 3 different geographic regions].		1977	204979
entamoeba histolytica: localization and characterization of phosphorylase and particulate glycogen.		1977	196880
entamoeba histolytica: localization and characterization of phosphoglucomutase, uridine diphosphate glucose pyrophosphorylase, and glycogen synthase.		1977	196881
structural changes on entamoeba histolytica trophozoites after cryopreservation in liquid nitrogen.	trophozoites of entamoeba histolytica cultures which had been deep-frozen in the presence of 5% dmso, along with untreated cells and cells treated with dmso (5%), were examined for fine-structural changes. after deep-freezing in liquid nitrogen only a few amoebae exhibited normal nuclear and cytoplasmic structure. one frequently observed but unspecific finding pertaining to recovered cells is the separation of the cytoplasm into large vacuolated (coarse-granular) and electron-optically fine-gran ...	1977	204119
[the enzyme-linked immunosorbent assay (elisa) in the diagnosis of amoebiasis (author's transl)].	an enzyme-linked immunosorbent assay (elisa) technique as an aid to the detection of antibodies to entamoeba histolytica has been evaluated in four groups of 39 individuals. results were positive in 15 patients with amoebic dysentery harboring trophozoites of entamoeba histolytica, in their stools; negative in 6 patients with enteric symptoms whose faeces contained neither trophozoites nor cysts of entamoeba histolytica, in 8 patients with other parasitic diseases in whom clinical examination wa ...	1977	204261
the epidemiology of infection with entamoeba histolytica in the gambia, west africa.	a country-wide stool survey of the gambia showed the prevalence of entamoeba histolytica cysts to range from 13.7% up-country in the dry season to 52.3% near the coast. a longitudinal survey showed a near 100% infection rate over one year and a sharp rise in prevalence as the rains commence with an equally sharp fall as the rains progress. specific antibody levels are elevated and reasonably constant through the year. carriers generally show no specific lymphocyte reactivity to amoebic antigen b ...	1977	202045
amoebiasis and intestinal parasitic infections in a population on cebu island, the philippines.		1977	202206
entamoeba histolytica and entamoeba invadens: chromium release from labeled human liver cells in culture.		1977	202477
isolation and maintenance of a strain of entamoeba histolytica schaudinn, 1903 and the possibility of its utilization in screening of drugs.		1977	203016
chemotherapeutically active nitro compounds. 4. 5-nitroimidazoles (part i).	more than 135 new 2-methyl-5-nitroimidazoles substituted in 1-position and 1-methyl-5-nitroimidazoles substituted in 2-position were investigated for their activity against various protozoan species, in particular entamoeba histolytica in the golden hamster, trichomonas fetus, trypanosoma brucei and t. cruzi in the nmri mouse. among the nitroimidazoles substituted in the 1-position only two preparations exhibited a similar effect as metronidazole preparations exhibited a similar effect as metron ...	1977	203293
viruses of entamoeba histolytica. vii. novel beaded virus.	a third amoebal virus type was isolated from four different strains of entamoeba histolytica. the virus was most frequently seen as a linear structure about 235 nm long and consisting of 14 beadlike structures about 19 nm in diameter. a "dimer" of twice the length and consisting of 28 beads was occationally encountered. the virus replicated in the nucleus, forming ordered arrays. acridine orange staining of viral aggregates in infected nuclei suggested the presence of double-stranded nucleic aci ...	1977	197265
amebiasis. an increasing problem among homosexuals in new york city.	during a five-year period at the new york hospital, entamoeba histolytica was identified in the stools of 20 men who had not traveled outside the new york area. all of the patients were found subsequently to homosexual. during this same period amebiasis was diagnosed in 30 men who had traveled; only two were homosexual. of ten patients with e histolytica infection seen during the first year of this study, none were homosexual whereas eight of 11 patients in the fifth year were homosexual, sugges ...	1977	197276
venereal transmission of enteric pathogens in male homosexuals. two case reports.	in two cases there was simultaneous or sequential occurrence of amebiasis, shigellosis, and giardiasis in male homosexuals. enteric pathogens may, under the proper conditions, be venereally transmitted. in particular, the sexual practices of male homosexuals, most significantly, oral-anal contact, appear to provide the necesseary link for transmission. evidence suggests that this is a growing problem.	1977	197277
[ameboma of the cecum].		1977	197436
[the origin of histological changes in amebiasis].	on microscopic examination of histological specimens from an autopsy case with severe amoebiasis, extensive ulcerative-necrotic lesions in the colon were found associated with an amoebic peritonitis and severe degenerative lesions of the ganglion-cells of the plexus myentericus induced by entamoeba histolytica. there was also an infiltration of a mesenteric lymphnode by amoebae carried by the lymph through the vasa afferentiae lymphaticae. in the liver several granulomas (amoebic granulomas) and ...	1977	193418
entamoeba histolytica: autoradiographic analysis of nuclear sites of rna synthesis.		1977	193710
effect of storage, different media constituents and dilution of axenic medium on the growth of axenic culture of entamoeba histolytica.		1977	193783
isolation of enteric pathogens from asymptomatic students from the united states and latin america.		1977	193996
actin in entamoeba histolytica trophozoites revealed by human actin antibodies.		1977	194025
[clinical aspects of amebiasis].		1977	194302
[biological diagnosis of malaria and amebiasis].		1977	194303
definition of the antigenic pattern of entamoeba histolytica, and immunoelectrophoretic analysis of the variation of patient response to amebic disease.	the antigenic anatomy of entamoeba histolytica and the variation in response of 148 patients with amebic disease are described. immunoelectrophoretic patterns and indirect hemagglutination titers were studied in sera obtained from patients with amebic disease acquired in several areas of the world, i.e. canada, vietnam, afric, india, central america, and southern united states. the various populations are compared and similarities and differences are discussed.	1977	194490
experimental amebiasis. i. pathogenicity of axenically cultured entamoeba histolytica in the brain of the newborn mouse.	axenically cultured entamoeba histolytica inoculated intracerebrally into newborn swiss mice invaded the cerebrum and produced multiple abscesses containing viable trophozoites. as few as 20 amebae of a virulent strain (hm-1:imss) occasionally produced fatal disease, 200 killed about 75% of animals and higher doses regularly killed all animals. in contrast, avirulent strains (hk-9 and hb-301:nih) failed to produce acute brain disease in comparable time periods even when mice were inoculated with ...	1977	194491
experimental amebiasis. ii. hepatic amebiasis in the newborn hamster.	newborn hamsters inoculated intrahepatically were highly susceptible to infection by axenically cultured entamoeba histolytica. inoculations were performed through the abdominal wall, and lesions could be observed through the skin as early as 4 days after inoculation. the most virulent amebal strain, hm-1:imss, produced liver lesions in 19% of newborn animals inoculated with 20 amebae, and in about 90% receiving 2,000 amebae. eleven other strains similarly tested either produced no lesions with ...	1977	194492
invasive amebiasis. i. amebic dysentery and its complications.		1977	195178