Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| il-23 is required for protection against systemic infection with listeria monocytogenes. | listeria monocytogenes (lm) is a gram-positive, intracellular bacterium that can induce spontaneous abortion, septicemia, and meningitis. although it is known that neutrophils are required for elimination of the bacteria and for survival of the host, the mechanisms governing the recruitment of neutrophils to lm-infected tissues are not fully understood. we demonstrate here that il-23 and the il-17 receptor a (il-17ra), which mediates both il-17a and il-17f signaling, are necessary for resistance ... | 2009 | 19923464 |
| [a case of listeria meningitis showed high levels of adenosine deaminase in cerebrospinal fluid]. | an 83-year-old woman developed high fever and headache for four days. she had disturbance of consciousness and was admitted to our hospital. a lumbar puncture was performed and cerebrospinal fluid (csf) analysis revealed a white blood cell count of 268/mm3 and culture became positive for listeria monocytogenes. six days after admission, the adenosine deaminase (ada) level in csf markedly elevated (43.3 iu/l) with pleocytosis, but a negative pcr test for tuberculosis in csf, sputum, gastric fluid ... | 2009 | 19928691 |
| infectious encephalitis in france in 2007: a national prospective study. | encephalitis is associated with significant mortality and morbidity, but its cause remains largely unknown. we designed a national prospective study in france in 2007 to describe patients with encephalitis, investigate the etiologic diagnosis of encephalitis, and assess risk factors associated with death. | 2009 | 19929384 |
| encephalitis: why we need to keep pushing the envelope. | 2009 | 19929385 | |
| a trans-acting riboswitch controls expression of the virulence regulator prfa in listeria monocytogenes. | riboswitches are rna elements acting in cis, controlling expression of their downstream genes through a metabolite-induced alteration of their secondary structure. here, we demonstrate that two s-adenosylmethionine (sam) riboswitches, srea and sreb, can also function in trans and act as noncoding rnas in listeria monocytogenes. srea and sreb control expression of the virulence regulator prfa by binding to the 5'-untranslated region of its mrna. absence of the sam riboswitches srea and sreb incre ... | 2009 | 19914169 |
| a listeria monocytogenes strain is still virulent despite nonfunctional major virulence genes. | the low-virulence listeria monocytogenes strains have been previously assigned to 4 phenotypic groups. this study aimed to characterize the a23 strain, which exhibits a pulsed-field gel electrophoresis profile specific to low-virulence strains. this strain has the same causal mutations as the group iii strains and a supplementary mutation in the mpl gene, leading to the absence of internalin a expression and the presence of inactive internalin b, phosphatidyl-inositol phospholipase c, and phosph ... | 2009 | 19911993 |
| fabh selectivity for anteiso branched-chain fatty acid precursors in low-temperature adaptation in listeria monocytogenes. | gram-positive bacteria, including listeria monocytogenes, adjust membrane fluidity by shortening the fatty acid chain length and increasing the proportional production of anteiso fatty acids at lower growth temperatures. the first condensation reaction in fatty acid biosynthesis is carried out by beta-ketoacyl-acyl carrier protein synthase iii (fabh), which determines the type of fatty acid produced in bacteria. here, we measured the initial rates of fabh-catalyzed condensation of malonyl-acyl c ... | 2009 | 19863661 |
| chemical composition and antimicrobial and antioxidant activities of mentha (longifolia l. and viridis) essential oils. | the study was aimed to investigate essential oil chemical composition (gas chromatography/flame ionization detection [gc-fid] and gas chromatography/mass spectrometry [gc-ms]) and antioxidant (1,1-diphenyl-2-picrylhydrazyl free radical (dpph) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate [abts] assays) and antimicrobial (gram-positive and gram-negative bacteria, fungi, and yeast) activities of essential oils extracted from leaves of mentha longifolia l. and mentha viridis. gc-ms analysis ... | 2009 | 19895481 |
| effects of allspice, cinnamon, and clove bud essential oils in edible apple films on physical properties and antimicrobial activities. | essential oils (eos) derived from plants are rich sources of volatile terpenoids and phenolic compounds. such compounds have the potential to inactivate pathogenic bacteria on contact and in the vapor phase. edible films made from fruits or vegetables containing eos can be used commercially to protect food against contamination by pathogenic bacteria. eos from cinnamon, allspice, and clove bud plants are compatible with the sensory characteristics of apple-based edible films. these films could e ... | 2009 | 19895483 |
| antibacterial effects of allspice, garlic, and oregano essential oils in tomato films determined by overlay and vapor-phase methods. | physical properties as well as antimicrobial activities against escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes of allspice, garlic, and oregano essential oils (eos) in tomato puree film-forming solutions (tpffs) formulated into edible films at 0.5% to 3% (w/w) concentrations were investigated in this study. antimicrobial activities were determined by 2 independent methods: overlay of the film on top of the bacteria and vapor-phase diffusion of the antimicrobial from th ... | 2009 | 19895486 |
| safety and functional aspects of preselected enterococci for probiotic use in iberian dry-fermented sausages. | the purpose of this study was to investigate enterococci for potential probiotic use in iberian dry-fermented sausages. a total of 15 strains isolated from iberian dry-fermented sausages, human feces, and pig feces were evaluated for their safety and functional characteristics including biogenic amine (ba) production, antibiotic susceptibility, hemolysis, virulence determinants, cell adhesion, and antimicrobial activity against foodborne pathogens. the strain enterococcus faecium se906 was able ... | 2009 | 19895487 |
| gammadelta t lymphocytes producing ifngamma and il-17 in response to candida albicans or mycobacterial antigens: possible implications for acute and chronic inflammation. | t lymphocytes bearing the gammadelta t cell receptor are known to play an important role in the first-line defense against viral, bacterial and fungal pathogens. two main subsets of gammadelta t cells are known, showing distinct functional behaviour: vdelta2 t lymphocytes, circulating in the peripheral blood, are involved in the response to mycobacterial infections and certain viruses, including coxsakie virus b3 and herpes simplex virus type 2. vdelta1 t cells are resident in the mucosal-associ ... | 2009 | 19903136 |
| assuring growth inhibition of listerial contamination during processing and storage of traditional greek graviera cheese: compliance with the new european union regulatory criteria for listeria monocytogenes. | the current microbiological regulatory criteria in the european union specify a maximum listeria monocytogenes population of 100 cfu/g allowable in ready-to-eat foods provided the product will not exceed this limit throughout its shelf life. the aim of this study was to validate the manufacturing method for traditional greek graviera cheese produced from thermized milk. initial challenge experiments evaluated the fate of inoculated l. monocytogenes (ca. 4 log cfu/ml, three-strain cocktail) in th ... | 2009 | 19903387 |
| efficacy of chlorine dioxide against listeria monocytogenes in brine chilling solutions. | chilled brine solutions are used by the food industry to rapidly cool ready-to-eat meat products after cooking and before packaging. chlorine dioxide (clo(2)) was investigated as an antimicrobial additive to eliminate listeria monocytogenes. several experiments were performed using brine solutions made of sodium chloride (nacl) and calcium chloride (cacl(2)) inoculated with l. monocytogenes and/or treated with 3 ppm of clo(2). first, 10 and 20% cacl(2) and nacl solutions (ph 7.0) were inoculated ... | 2009 | 19903388 |
| morphology, release characteristics, and antimicrobial effect of nisin-loaded electrospun gelatin fiber mat. | gelatin electrospun (e-spun) fiber mats containing nisin were produced by electrostatic spinning of gelatin-nisin in 70% (vol/vol) acetic acid aqueous solutions. varying nisin loading concentration (0 to 3% [wt/wt]) did not affect the fiber average diameter, whereas increasing gelatin concentration from 20 to 24% (wt/vol) caused an increase in the average diameter. all nisin-loaded gelatin e-spun fiber mats demonstrated inhibition against lactobacillus plantarum tistr 850. however, all fiber mat ... | 2009 | 19903391 |
| prevalence and molecular diversity of listeria monocytogenes in retail establishments. | as our understanding of listeria monocytogenes transmission in retail and deli operations is limited, we conducted a cross-sectional study of l. monocytogenes contamination patterns in 121 retail establishments, using testing of food and environmental samples and subtype analysis (ribotyping) of l. monocytogenes isolates. seventy-three (60%) establishments had at least one sample that tested positive for l. monocytogenes; 5 (2.7%) of the 183 food and 151 (13.0%) of the 1,161 environmental sample ... | 2009 | 19903398 |
| pkctheta is required for alloreactivity and gvhd but not for immune responses toward leukemia and infection in mice. | when used as therapy for hematopoietic malignancies, allogeneic bm transplantation (bmt) relies on the graft-versus-leukemia (gvl) effect to eradicate residual tumor cells through immunologic mechanisms. however, graft-versus-host disease (gvhd), which is initiated by alloreactive donor t cells that recognize mismatched major and/or minor histocompatibility antigens and cause severe damage to hematopoietic and epithelial tissues, is a potentially lethal complication of allogeneic bmt. to enhance ... | 2009 | 19907075 |
| [prevalence of listeria monocytogenes in fresh tomatoes (lycopersicum esculentum) and coriander (coriandrum sativum) in three markets of valencia, venezuela]. | the incidence of l. monocytogenes in tomatoes and coriander obtained from three different markets, during eight weeks were determined. 192 samples were evaluated: 96 of tomatoes, and 96 of coriander. the isolation of l. monocytogenes was performed using covenin 3718:2001. the data were analyzed by spss version 12.0. kolmogorov smirnov, mann whitney u, kruskal wallis u test; spearman's correlation were applied, and p<0.05 significance level was aplied. it was not found significant differences bet ... | 2009 | 19886518 |
| risk of fetal mortality after exposure to listeria monocytogenes based on dose-response data from pregnant guinea pigs and primates. | one-third of the annual cases of listeriosis in the united states occur during pregnancy and can lead to miscarriage or stillbirth, premature delivery, or infection of the newborn. previous risk assessments completed by the food and drug administration/the food safety inspection service of the u.s. department of agriculture/the centers for disease control and prevention (fda/usda/cdc) and food and agricultural organization/the world health organization (fao/who) were based on dose-response data ... | 2009 | 19886944 |
| cutting edge: memory cd8 t cell compartment grows in size with immunological experience but nevertheless can lose function. | the size of the adaptive immune system is considered to be kept constant by the attrition of pre-existing memory. however, recently it was shown that the cd8 memory compartment can grow in size and the number of pre-existing memory is largely preserved, predicting that pre-existing immunity should be maintained (vezys et al.; nature 457: 196-199). experimental proof for this assumption is still lacking. we address this question in the listeria monocytogenes (l.m.) infection model and confirm the ... | 2009 | 19890045 |
| real-time pcr detection of five prevalent bacteria causing acute meningitis. | 2009 | 19845537 | |
| selenium deficiency impairs host innate immune response and induces susceptibility to listeria monocytogenes infection. | susceptibility or resistance to infection with listeria monocytogenes correlates with selenium (se) deficiency in response to infection. | 2009 | 19852827 |
| listeria monocytogenes acta is a key player in evading autophagic recognition. | autophagy is a pivotal bulk degradation system that eliminates undesirable molecules, damaged organelles, and misfolded protein aggregates in response to diverse stimuli, including infection. autophagy acts to limit intracellular microbial growth but intracellular pathogens have evolved strategies to subvert host autophagic responses for their survival. we found that listeria monocytogenes acta, a surface protein required for actin polymerization and actin-based bacterial motility, plays a pivot ... | 2009 | 19855178 |
| tnfr2-deficient memory cd8 t cells provide superior protection against tumor cell growth. | tnf receptor-2 (tnfr2) plays a critical role in promoting the activation and survival of naive t cells during the primary response. interestingly, anti-cd3 plus il-2 activated tnfr2(-/-) cd8 t cells are highly resistant to activation-induced cell death (aicd), which correlates with high expression levels of prosurvival molecules such as bcl-2, survivin, and cd127 (il-7ralpha). we determined whether the resistance of activated tnfr2(-/-) cd8 t cells to aicd contributes to more effective protectio ... | 2009 | 19841176 |
| fat content increases the lethality of ultra-high-pressure homogenization on listeria monocytogenes in milk. | listeria monocytogenes ccug 15526 was inoculated at a concentration of approximately 7.0 log(10) cfu/ml in milk samples with 0.3, 3.6, 10, and 15% fat contents. milk samples with 0.3 and 3.6% fat content were also inoculated with a lower load of approximately 3.0 log(10) cfu/ml. inoculated milk samples were subjected to a single cycle of ultra-high-pressure homogenization (uhph) treatment at 200, 300, and 400 mpa. microbiological analyses were performed 2 h after the uhph treatments and after 5, ... | 2009 | 19841200 |
| preexposure to repeated low doses of zymosan increases the susceptibility to pulmonary infection in rats. | chronic exposure to low levels of mold has been reported to increase susceptibility to respiratory infections. in the current study, the authors investigate the lungs' ability to clear an infection after repeated low-dose zymosan exposure. exposure was conducted at a zymosan dose of 0.6 mg/kg body weight (bw) of rat, for a total of 4 doses, via intratracheal instillation during a 2 week period. treated animals were allowed to recover for 1 week before pulmonary inoculation with listeria monocyto ... | 2009 | 19842846 |
| fulminant listerial infection of the central nervous system in an otherwise healthy patient: a case report. | the mortality of listerial rhombo-encephalitis exceeds 26% and may involve otherwise healthy patients. a case is presented of a man with fatal listerial infection of the central nervous system that was monitored in an intensive care unit. | 2009 | 19830196 |
| bioactivities of plectranthus ecklonii constituents. | plectranthus ecklonii benth. is traditionally used in south africa for treating stomach aches, nausea, vomiting and meningitis. bioassay-guided fractionation of the ethyl acetate extract of the plant led to the isolation of two known compounds, parvifloron d and parvifloron f, neither of which has been previously reported for this species. the compounds exhibited minimum inhibitory concentrations of 15.6 and 31.2 microg/ml, respectively against listeria monocytogenes, whereas the values against ... | 2009 | 19831023 |
| adherence characteristics of listeria strains isolated from three ready-to-eat meat processing plants. | over 1,560 non-food contact surface swabs and raw meat ingredient samples were collected from three ready-to-eat meat processing plants (520 from each plant) from 1998 to 1999, resulting in the recovery of 259 isolates of listeria obtained from postprocess areas including drains, floors, garbage bins, cart wheels, walls, equipment surfaces, tables, brooms, pallet jacks, hoses, ladders, and waste chutes. we further examined 246 of the 259 isolates for adherence phenotype and used pcr to identify ... | 2009 | 19833036 |
| development and validation of an extensive growth and growth boundary model for listeria monocytogenes in lightly preserved and ready-to-eat shrimp. | an existing cardinal parameter growth and growth boundary model for listeria monocytogenes (o. mejlholm and p. dalgaard, j. food prot. 70:70-84 and 2485-2497, 2007) was expanded with terms for the effects of acetic, benzoic, citric, and sorbic acids to include a total of 12 environmental parameters and their interactive effects. the new model predicted growth rates (micro(max) values) of l. monocytogenes accurately with bias and accuracy factors of 1.0 and 1.5, respectively, for 16 batches of br ... | 2009 | 19833037 |
| dose of uv light required to inactivate listeria monocytogenes in distilled water, fresh brine, and spent brine. | the purpose of this research was to establish the dose of uv light (253.7 nm) needed to inactivate listeria monocytogenes in distilled water, fresh brine (9% nacl), spent brine, and diluted (5, 35, and 55%) spent brine, using uridine as a chemical actinometer. strains n1-227 (isolated from hot dog batter), n3-031 (isolated from turkey franks), and r2-499 (isolated from meat) were mixed in equal proportions and suspended in each solution prepared so as to contain 10(-4) m uridine. samples were ir ... | 2009 | 19833038 |
| isolation and 2-d-dige proteomic analysis of intracellular and extracellular forms of listeria monocytogenes. | the pathogenicity of listeria monocytogenes is related to its ability of invading and multiplying in eukaryotic cells. its main virulence factors are now well characterized, but limited proteomic data is available concerning its adaptation to the intracellular environment. in this study, l. monocytogenes egd (serotype 1/2a) grown in human thp-1 monocytes (24 h) were successfully separated from host organelles and cytosolic proteins by differential and isopycnic centrifugation. for control, we us ... | 2009 | 19834917 |
| [characterization of a naturally atypical listeria monocytogenes strain lacking inlab]. | we attempted to gain insights into the phenotypic and genetic characteristics of a naturally atypical listeria monocytogenes strain s10 lacking inlab. | 2009 | 19835164 |
| differential effects of cytokines and corticosteroids on toll-like receptor 2 expression and activity in human airway epithelia. | the recognition of microbial molecular patterns via toll-like receptors (tlrs) is critical for mucosal defenses. | 2009 | 19835594 |
| comparison of different application strategies of divergicin m35 for inactivation of listeria monocytogenes in cold-smoked wild salmon. | cold-smoked salmon treated with divergicin m35-producing carnobacterium divergens m35, c. divergens atcc 35677 (a non-producer of bacteriocin), purified divergicin m35 or supernatants of c. divergens m35 culture in snow crab hepatopancreas (sch) medium or mrs broth was challenged with listeria monocytogenes (up to 10(3) cfu/g). samples were stored at 4 degrees c for up to four weeks. l. monocytogenes, total bacterial and lactic acid bacterial counts were determined along with changes in total vo ... | 2009 | 19835762 |
| inhibition by lactobacillus sakei of other species in the flora of vacuum packaged raw meats during prolonged storage. | the abilities of five lactobacillus sakei strains and one lactococcus lactis strain to retain inhibitory activity against several target organisms in the flora of product during 12 weeks storage of vacuum-packaged lamb and beef was investigated. l. sakei strains were generally found capable of developing dominant populations on both beef and lamb. l. lactis 75 grew poorly on lamb did not inhibit co-inoculated brochothrix thermosphacta. lamb inoculated with the sakacin-a producer l. sakei lb706 h ... | 2009 | 19835775 |
| resistance of listeria monocytogenes, escherichia coli o157:h7 and campylobacter jejuni after exposure to repetitive cycles of mild bactericidal treatments. | while maintaining nutritional and sensorial attributes of fresh foods mild processing technologies generally deliver microbiologically perishable food products. currently little information exists on possible increase in the resistance of pathogens after repetitive exposure to mild (sub-lethal) treatments. multiple strain-cocktails of listeria monocytogenes, escherichia coli o157:h7 and campylobacter jejuni were exposed to 20 consecutive cycles of sub-lethal inactivation by three different techn ... | 2009 | 19835777 |
| comparative analysis of acid resistance in listeria monocytogenes and salmonella enterica strains before and after exposure to poultry decontaminants. role of the glutamate decarboxylase (gad) system. | data on the ability of chemical poultry decontaminants to induce an acid stress response in pathogenic bacteria are lacking. this study was undertaken in order to compare the survival rates in acid broths of listeria monocytogenes and salmonella enterica strains, both exposed to and not exposed to decontaminants. the contribution of the glutamate decarboxylase (gad) acid resistance system to the survival of bacteria in acid media was also examined. four strains (l. monocytogenes serovar 1/2, l. ... | 2009 | 19835779 |
| occurrence of foodborne pathogens in irish farmhouse cheese. | food safety is a critical factor in the production of farmhouse cheese. in ireland the varieties of farmhouse cheese produced reflect a much broader range than those produced commercially and some of these cheese varieties are associated with greater microbiological risk. these include cheese produced from unpasteurised milk and soft ripened cheese such as mould or smear-ripened cheeses which have high ph and relatively short ripening times. the aim of this study was to determine the microbiolog ... | 2009 | 19835780 |
| insertional mutagenesis of listeria monocytogenes 568 reveals genes that contribute to enhanced thermotolerance. | the objectives of this study were to identify molecular mechanisms of thermotolerance using transposon mutants of listeria monocytogenes 568, serotype 1/2a, and to compare their thermal death kinetics at 52, 56 and 60 degrees c. sixteen tn917 transposon mutants with enhanced heat resistance were acquired from a library of 4300 mutants following a multi-step screening process. genetic regions with tn917 insertions encompassed a broad range of functionalities including; transport, metabolism, repl ... | 2009 | 19836093 |
| diamide triggers mainly s thiolations in the cytoplasmic proteomes of bacillus subtilis and staphylococcus aureus. | glutathione constitutes a key player in the thiol redox buffer in many organisms. however, the gram-positive bacteria bacillus subtilis and staphylococcus aureus lack this low-molecular-weight thiol. recently, we identified s-cysteinylated proteins in b. subtilis after treatment of cells with the disulfide-generating electrophile diamide. s cysteinylation is thought to protect protein thiols against irreversible oxidation to sulfinic and sulfonic acids. here we show that s thiolation occurs also ... | 2009 | 19837798 |
| genetic features of resident biofilms determine attachment of listeria monocytogenes. | planktonic listeria monocytogenes cells in food-processing environments tend most frequently to adhere to solid surfaces. under these conditions, they are likely to encounter resident biofilms rather than a raw solid surface. although metabolic interactions between l. monocytogenes and resident microflora have been widely studied, little is known about the biofilm properties that influence the initial fixation of l. monocytogenes to the biofilm interface. to study these properties, we created a ... | 2009 | 19837841 |
| microbial induction of inflammatory bowel disease associated gene tl1a (tnfsf15) in antigen presenting cells. | tl1a is a member of the tnf superfamily and its expression is increased in the mucosa of inflammatory bowel disease patients. neutralizing anti-mouse tl1a ab attenuates chronic colitis in two t-cell driven murine models, suggesting that tl1a is a central modulator of gut mucosal inflammation in inflammatory bowel disease. we showed previously that tl1a is induced by immune complexes via the fc gamma r signaling pathway. in this study, we report that multiple bacteria, including gram negative org ... | 2009 | 19839006 |
| [listeriosis, a rare but severe foodborne infection]. | listeria monocytogenes is a gram-positive bacillus widespread in nature and responsible for human and animal infections. listeriosis is a severe and rare infection transmitted orally and vertically, frequently in the context of an underlying immunosuppression. l. monocytogenes is able to cross several barriers: intestinal mucosa, placenta and blood-brain barrier. listeriosis is associated with a range of clinical presentations: ranging from acute gastroenteritis, septicaemia, central nervous sys ... | 2009 | 19839454 |
| effects of bilimbi (averrhoa bilimbi l.) and tamarind (tamarindus indica l.) juice on listeria monocytogenes scott a and salmonella typhimurium atcc 14028 and the sensory properties of raw shrimps. | the potential of using juice of bilimbi (averrhoa bilimbi l.) and tamarind (tamarindus indica l.) to reduce listeria monocytogenes scott a and salmonella typhimurium atcc 14028 populations on raw shrimps after washing and during storage (4 degrees c) was investigated. the uninoculated raw shrimps and those inoculated with approximately 9 log cfu/ml of l. monocytogenes scott a and s. typhimurium atcc 14028 were washed (dipped or rubbed) in distilled water (sdw) (control), bilimbi or tamarind juic ... | 2009 | 19818521 |
| mast cell-mediated antigen presentation regulates cd8+ t cell effector functions. | the characteristics, importance, and molecular requirements for interactions between mast cells (mcs) and cd8(+) t cells have not been elucidated. here, we demonstrated that mcs induced antigen-specific cd8(+) t cell activation and proliferation. this process required direct cell contact and mhc class i-dependent antigen cross-presentation by mcs and induced the secretion of interleukin-2, interferon-gamma, and macrophage inflammatory protein-1alpha by cd8(+) t cells. mcs regulated antigen-speci ... | 2009 | 19818652 |
| analyzing the power and error of listeria monocytogenes growth challenge studies. | domestic and international food safety policy developments have spurred interest in the design and interpretation of experimental growth challenge studies to determine whether ready-to-eat (rte) foods are able to support growth of listeria monocytogenes. existing challenge study protocols and those under development differ markedly in terms of experimental design and the acceptance criteria under which a rte food is determined not to support l. monocytogenes growth. consequently, the protocols d ... | 2009 | 19822374 |
| listeria as an enteroinvasive gastrointestinal pathogen. | the bacterium listeria monocytogenes is the causative agent of listeriosis, a highly fatal opportunistic foodborne infection. listeria spp. are isolated from a diversity of environmental sources, including soil, water, effluents, a large variety of foods, and the feces of humans and animals. recent outbreaks demonstrated that l. monocytogenes can cause gastroenteritis in otherwise healthy individuals and more severe invasive disease in immunocompromised patients. common symptoms include fever, w ... | 2009 | 19812983 |
| listeria monocytogenes ctap is a multifunctional cysteine transport-associated protein required for bacterial pathogenesis. | the bacterial pathogen listeria monocytogenes survives under a myriad of conditions in the outside environment and within the human host where infections can result in severe disease. bacterial life within the host requires the expression of genes with roles in nutrient acquisition as well as the biosynthesis of bacterial products required to support intracellular growth. a gene product identified as the substrate-binding component of a novel oligopeptide transport system (encoded by lmo0135) wa ... | 2009 | 19818015 |
| transcriptional and post-transcriptional regulation of the gmar antirepressor governs temperature-dependent control of flagellar motility in listeria monocytogenes. | flagellar motility in listeria monocytogenes (lm) is restricted to temperatures below 37 degrees c due to the opposing activities of the mogr transcriptional repressor and the gmar antirepressor. previous studies have suggested that both the degu response regulator and mogr regulate expression of gmar. in this report, we further define the role of degu for gmar production and flagellar motility. we demonstrate that deletion of the receiver domain of degu has no effect on flagellar motility in lm ... | 2009 | 19796338 |
| cutting edge: importance of il-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with b lymphocytes. | b lymphocytes are a potential alternative to dendritic cell immunotherapy, with the advantages of relative abundance in peripheral blood and the ability to function as apcs. although b cells express multiple receptors that induce costimulatory molecules, b cell vaccine studies have focused primarily on cd40 stimulation. to optimize the potential efficacy of b cell vaccines (bvac), we compared the capacity of differentially stimulated b cells to induce ag-specific cd8(+) t cell responses in vivo. ... | 2009 | 19801508 |
| autophagy in immunity against intracellular bacteria. | autophagy is an innate immune defense mechanism against various intracellular bacterial pathogens, such as salmonella enterica serovar typhimurium (s. typhimurium), listeria monocytogenes and shigella flexneri. s. typhimurium uses type three secretion systems (t3sss) to invade mammalian cells and replicate in salmonella-containing vacuoles (scvs). a small population of intracellular s. typhimurium is targeted by autophagy shortly after infection. evidence suggests that these bacteria are present ... | 2009 | 19802566 |
| dendritic cell cross-priming is essential for immune responses to listeria monocytogenes. | cross-presentation is now recognized as a major mechanism for initiating cd8 t cell responses to virus and tumor antigens in vivo. it provides an elegant mechanism that allows relatively few dendritic cells (dcs) to initiate primary immune responses while avoiding the consumptive nature of pathogenic infection. cd8 t cells play a major role in anti-bacterial immune responses; however, the contribution of cross-presentation for priming cd8 t cell responses to bacteria, in vivo, is not well establ ... | 2009 | 19806187 |
| inactivation of the wall-associated de-n-acetylase (pgda) of listeria monocytogenes results in greater susceptibility of the cells to induced autolysis. | several species of gram-positive bacteria have cell wall peptidoglycan (syn. murein) in which not all of the sugar moieties are n-acetylated. this has recently been shown to be a secondary effect, caused by the action of a peptidoglycan n-acetylglucosamine deacetylase. we have found that the opportunistic pathogen listeria monocytogenes is unusual in having three enzymes with such activity, two of which remain in the cytoplasm. here, we examine the enzyme (pgda) that crosses the cytoplasmic memb ... | 2009 | 19809250 |
| pcr experion automated electrophoresis system to detect listeria monocytogenes in foods. | listeria monocytogenes is frequently found as a contaminant in raw and ready-to-eat foods. the ability of l. monocytogenes to multiply at refrigeration temperatures and to grow in a wide range of ph values is of particular concern for food safety. according to the european union regulation on microbiological criteria for foodstuffs, l. monocytogenes must be absent in some categories of ready-to-eat foods. the standard microbiological method for l. monocytogenes detection in foods (iso 11290-1: 1 ... | 2009 | 19810054 |
| neonatal immunization with listeria monocytogenes induces t cells with an adult-like avidity, sensitivity, and tcr-vbeta repertoire, and does not adversely impact the response to boosting. | listeria monocytogenes (lm) holds promise as a neonatal vaccine vehicle. here we show that lm immunized neonatal mice reached maximal ag-specific cd8(+) t cell expansion after only a single immunization, while adults required two doses. ag-specific cd4(+) t cell expansion in both age groups required a boost to reach its peak. neither functional avidity, sensitivity, nor the tcr-vbeta repertoire of the ag-specific t cells differed between mice immunized as neonates or adults. lastly, neonatal imm ... | 2009 | 19796722 |
| superior protective immunity against murine listeriosis by combined vaccination with cpg dna and recombinant salmonella enterica serovar typhimurium. | preexisting antivector immunity can severely compromise the ability of salmonella enterica serovar typhimurium live vaccines to induce protective cd8 t-cell frequencies after type iii secretion system-mediated heterologous protein translocation in orally immunized mice. to circumvent this problem, we injected cpg dna admixed to the immunodominant p60(217-225) peptide from listeria monocytogenes subcutaneously into balb/c mice and coadministered a p60-translocating salmonella strain by the orogas ... | 2009 | 19797070 |
| inactivation of listeria monocytogenes on unpackaged and vacuum-packaged chicken frankfurters using pulsed uv-light. | the effectiveness of pulsed uv-light on the microbial load and quality of unpackaged and vacuum-packaged chicken frankfurters was investigated. samples were inoculated with listeria monocytogenes scott a on the top surfaces, and then treated with pulsed uv-light for 5, 15, 30, 45, and 60 s at 5, 8, and 13 cm distance from the quartz window in a pulsed uv-light chamber. log reductions (cfu/cm(2)) on unpackaged samples were between 0.3 and 1.9 after 5-s treatment at 13 cm and 60-s treatment at 5 c ... | 2009 | 19799670 |
| edible apple film wraps containing plant antimicrobials inactivate foodborne pathogens on meat and poultry products. | apple-based edible films containing plant antimicrobials were evaluated for their activity against pathogenic bacteria on meat and poultry products. salmonella enterica or e. coli o157:h7 (10(7) cfu/g) cultures were surface inoculated on chicken breasts and listeria monocytogenes (10(6) cfu/g) on ham. the inoculated products were then wrapped with edible films containing 3 concentrations (0.5%, 1.5%, and 3%) of cinnamaldehyde or carvacrol. following incubation at either 23 or 4 degrees c for 72 ... | 2009 | 19799671 |
| universal primer-multiplex pcr approach for simultaneous detection of escherichia coli, listeria monocytogenes, and salmonella spp. in food samples. | escherichia coli, listeria monocytogenes, and salmonella spp. are 3 kinds of the most important food-borne human pathogens. traditional microbiological analysis is labor-intensive, time-consuming, and easily contaminated, thus producing false positive signals; it also involves much subjectivity judgments. multiplex-pcr could be applied to detect multiple target organisms simultaneously to save time and labor, but there is always disproportionate amplification resulting from the disparity of diff ... | 2009 | 19799672 |
| microwave oven heating for inactivation of listeria monocytogenes on frankfurters before consumption. | microwave oven heating was evaluated for inactivation of listeria monocytogenes on inoculated and stored frankfurters. frankfurters formulated without/with 1.5% potassium lactate and 0.1% sodium diacetate were inoculated with l. monocytogenes (1.9 +/- 0.2 log cfu/cm(2)), vacuum-packaged, and stored (4 degrees c) to simulate conditions prior to purchase by consumers. at storage days 18, 36, and 54, packages were opened and placed at 7 degrees c, simulating aerobic storage in a household refrigera ... | 2009 | 19799673 |
| efficient oligonucleotide probe selection for pan-genomic tiling arrays. | array comparative genomic hybridization is a fast and cost-effective method for detecting, genotyping, and comparing the genomic sequence of unknown bacterial isolates. this method, as with all microarray applications, requires adequate coverage of probes targeting the regions of interest. an unbiased tiling of probes across the entire length of the genome is the most flexible design approach. however, such a whole-genome tiling requires that the genome sequence is known in advance. for the accu ... | 2009 | 19758451 |
| cellular pharmacokinetics and intracellular activity of torezolid (tr-700): studies with human macrophage (thp-1) and endothelial (huvec) cell lines. | optimal treatment of infections caused by staphylococcus aureus, listeria monocytogenes and legionella pneumophila requires antibiotics with intracellular activity. linezolid accumulates poorly within cells. torezolid (tr-700) is a novel methyltetrazolyl oxazolidinone with potentially different cellular pharmacokinetic properties. our aim was to examine the accumulation and intracellular activities of torezolid in this context. | 2009 | 19759040 |
| duox2-derived reactive oxygen species are effectors of nod2-mediated antibacterial responses. | generation of microbicidal reactive oxygen species (ros) is a pivotal protective component of the innate immune system in many eukaryotes. nod (nucleotide oligomerisation domain containing protein)-like receptors (nlrs) have been implicated as phylogenetically ancient sensors of intracellular pathogens or endogenous danger signals. nod2 recognizes the bacterial cell wall component muramyldipeptide leading to nfkappab and mapk activation via induced proximity signalling through the serine-threoni ... | 2009 | 19759286 |
| effect of bile salts on the dna and membrane integrity of enteric bacteria. | enteric bacteria are able to resist the high concentrations of bile encountered throughout the gastrointestinal tract. here we review the current mechanisms identified in the enteric bacteria salmonella, escherichia coli, bacillus cereus and listeria monocytogenes to resist the dangerous effects of bile. we describe the role of membrane transport systems, and their connection with dna repair pathways, in conferring bile resistance to these enterics. we discuss the findings from recent investigat ... | 2009 | 19762477 |
| control of listeria monocytogenes in turkey deli loaves using organic acids as formulation ingredients. | the growth of listeria monocytogenes in further-processed meat products has become a major concern and an important food safety issue. the meat and poultry industries have incorporated interventions such as organic acids in marinades to inhibit the growth of l. monocytogenes. in this study, organic acids were utilized in the raw product and as a postcook dip to determine their inhibitory effect on the growth of l. monocytogenes in turkey deli loaves. the turkey deli loaves were processed, cooked ... | 2009 | 19762881 |
| listeria monocytogenes attachment to and detachment from stainless steel surfaces in a simulated dairy processing environment. | the presence of pathogens in dairy products is often associated with contamination via bacteria attached to food-processing equipment, especially from areas where cleaning/sanitation is difficult. in this study, the attachment of listeria monocytogenes on stainless steel (ss), followed by detachment and growth in foods, was evaluated under conditions simulating a dairy processing environment. initially, ss coupons were immersed in milk, vanilla custard, and yogurt inoculated with the pathogen (1 ... | 2009 | 19767476 |
| complex phenotypic and genotypic responses of listeria monocytogenes strains exposed to the class iia bacteriocin sakacin p. | sakacin p is a class iia bacteriocin that is active against the food-borne pathogen listeria monocytogenes, and use of this compound as a biopreservative in foods has been suggested. in the present study, we characterized 30 spontaneous sakacin p-resistant mutants of l. monocytogenes obtained after single exposure to sakacin p. the frequency of development of sakacin p resistance for all strains was in the range from 10(-8) to 10(-9). using the 50% inhibitory concentration (ic(50)) of sakacin p, ... | 2009 | 19767478 |
| the bacterial virulence factor inlc perturbs apical cell junctions and promotes cell-to-cell spread of listeria. | several pathogenic bacteria, including listeria monocytogenes, use an f-actin motility process to spread between mammalian cells. actin 'comet tails' propel listeria through the cytoplasm, resulting in bacteria-containing membrane protrusions that are internalized by neighbouring cells. the mechanism by which listeria overcomes cortical tension to generate protrusions is unknown. here, we identify bacterial and host proteins that directly regulate protrusions. we show that efficient spreading be ... | 2009 | 19767742 |
| card9 facilitates microbe-elicited production of reactive oxygen species by regulating the lygdi-rac1 complex. | in response to invading microorganisms, macrophages engage in phagocytosis and rapidly release reactive oxygen species (ros), which serve an important microbicidal function. however, how phagocytosis induces ros production remains largely unknown. card9, a caspase-recruitment domain (card)-containing protein, is important for resistance to fungal and bacterial infection. the mechanism of card9-mediated bacterial clearance is still mostly unknown. here we show that card9 is required for killing i ... | 2009 | 19767757 |
| in silico reconstitution of actin-based symmetry breaking and motility. | eukaryotic cells assemble viscoelastic networks of crosslinked actin filaments to control their shape, mechanical properties, and motility. one important class of actin network is nucleated by the arp2/3 complex and drives both membrane protrusion at the leading edge of motile cells and intracellular motility of pathogens such as listeria monocytogenes. these networks can be reconstituted in vitro from purified components to drive the motility of spherical micron-sized beads. an elastic gel mode ... | 2009 | 19771152 |
| [spontaneous bacterial peritonitis and listeria monocytogenes]. | 2009 | 19775592 | |
| studies on the susceptibility of different culture morphotypes of listeria monocytogenes to uptake and survival in human polymorphonuclear leukocytes. | this study demonstrated that atypical virulent filaments of listeria monocytogenes (rough variant type ii and designated fr for this study), isolated from clinical specimens or generated during exposure to pulsed-plasma gas discharge in liquids, were shown to be capable of survival when engulfed by human polymorphonuclear leukocytes (pmnls). factors shown to significantly influence the maximal respiratory burst response in pmnls and survival of different internalized cell or filament forms of l. ... | 2009 | 19735470 |
| microbial contamination of milk and dairy products from restaurants in spain. | this study was carried out to evaluate the microbiological quality of milk and some dairy products, including 95 lots of warm milk, 95 lots of milk conserved at room temperature, and 75 lots of dairy products collected from restaurants in spain. according to the european microbiological criteria (92/46/eec, 93/43/eec, and commission regulation no. 2073/2005), 31% and 35% of the total examined lots exceed the adopted limits of mesophilic aerobic counts and enterobacteriaceae, respectively. this m ... | 2009 | 19737068 |
| specific osmolyte transporters mediate bile tolerance in listeria monocytogenes. | the food-borne pathogenic bacterium listeria monocytogenes has the potential to adapt to an array of suboptimal growth environments encountered within the host. the pathogen is relatively bile tolerant and has the capacity to survive and grow within both the small intestine and the gallbladder in murine models of oral infection. we have previously demonstrated a role for the principal carnitine transport system of l. monocytogenes (opuc) in gastrointestinal survival of the pathogen (r. sleator, ... | 2009 | 19737907 |
| in vitro activities of three new dihydrofolate reductase inhibitors against clinical isolates of gram-positive bacteria. | bal0030543, bal0030544, and bal0030545 are dihydrophthalazine inhibitors with in vitro potency against gram-positive pathogens. the mic(50)s for methicillin (meticillin)-sensitive staphylococcus aureus, methicillin-resistant staphylococcus aureus, hetero-vancomycin-resistant staphylococcus aureus, and vancomycin-resistant staphylococcus aureus (visa) range from 0.015 to 0.25 microg/ml (mic(90)s < or = 0.5 microg/ml). mic(50)s for beta-hemolytic streptococci range from 0.03 to 0.06 microg/ml, mic ... | 2009 | 19738027 |
| role of cybr, a cytohesin binder and regulator, in cd4(+) t-cell function and host immunity. | cytohesin binder and regulator (cybr) is known to regulate leukocyte adhesion and migration. however, its function in t-cells is poorly understood. here, we investigated the role of cybr in cd4(+) t-cell function and host immunity. cybr inhibited p38 phosphorylation following cd4(+) t-cell stimulation. since p38 regulates the expression of t-box expressed in t-cells (t-bet) but not gata binding protein 3 (gata-3) in t-cells, cybr decreased the expression of t-bet and ifn-gamma in cd4(+) t-cells. ... | 2009 | 19744714 |
| effect of nisin (nisaplin) on the growth of listeria monocytogenes in karashi-mentaiko (red-pepper seasoned cod roe). | the influence of nisaplin, which contains 2.5% nisin, on the growth of listeria monocytogenes in karashi-mentaiko (red-pepper seasoned cod roe) was investigated. the mics of nisaplin for l. monocytogenes (10(8) cfu/ml) were measured; seven isolates showed a value of 1,600 microg/ml and one isolate showed a value of 800 microg/ml. all l. monocytogenes isolates had a mic of 800 microg/ml at 10(6) cfu/ml. the number of l. monocytogenes in karashi-mentaiko stored at 4 degrees c was decreased by nisa ... | 2009 | 19745585 |
| p38 mapk-dependent phagocytic encapsulation confers infection tolerance in drosophila. | hosts employ a combination of two distinct yet compatible strategies to defend themselves against parasites: resistance, the ability to limit parasite burden, and tolerance, the ability to limit damage caused by a given parasite burden. animals typically exhibit considerable genetic variation in resistance to a variety of pathogens; however, little is known about whether animals can evolve tolerance. using a bacterial infection model in drosophila, we uncovered a p38 map kinase-mediated mechanis ... | 2009 | 19748466 |
| regulatory mimicry in listeria monocytogenes actin-based motility. | the actin-based motility of the intracellular pathogen listeria monocytogenes relies on acta, a bacterial factor with a structural domain allowing it to mimic the actin nucleation-promoting activity of host cell proteins of the wasp/wave family. here, we used an rnai-based genetic approach in combination with computer-assisted image analysis to investigate the role of host factors in l. monocytogenes cell-to-cell spread. we showed that the host cell serine/threonine kinase ck2 is required for ef ... | 2009 | 19748468 |
| listeria monocytogenes acta-mediated escape from autophagic recognition. | autophagy degrades unnecessary organelles and misfolded protein aggregates, as well as cytoplasm-invading bacteria. nevertheless, the bacteria listeria monocytogenes efficiently escapes autophagy. we show here that recruitment of the arp2/3 complex and ena/vasp, via the bacterial acta protein, to the bacterial surface disguises the bacteria from autophagic recognition, an activity that is independent of the ability to mediate bacterial motility. l. monocytogenes expressing acta mutants that lack ... | 2009 | 19749745 |
| priming and activation of human ovarian and breast cancer-specific cd8+ t cells by polyvalent listeria monocytogenes-based vaccines. | immunotherapeutic vaccine is potentially an effective strategy to combat cancer. essential components of an effective vaccine must include antigens that are processed by the major histocompatibility complex class i pathway, presented by the tumor major histocompatibility complex molecules, and an effective antigen delivery platform that is capable of breaking self-tolerance. in this study, we characterized a set of ovarian cancer-specific t-cell epitopes delivered by live-attenuated recombinant ... | 2009 | 19752748 |
| development of an escherichia coli expressing listeriolysin-o vaccine against wilms tumor gene 1-expressing tumors. | through their ability to induce cytotoxic t-lymphocytes and inhibit foxp3 t-regulatory cells, escherichia coli expressing listeriolysin-o (llo) and a model tumor antigen have been shown to exert strong antitumor activity. the aim of this study is to extend these observations to a self-protein and clinically relevant tumor antigen associated with most types of adult leukemia: wilms tumor gene 1 (wt1). we demonstrate that an e. coli coexpressing llo and wt1 is capable of inducing a strong antitumo ... | 2009 | 19752749 |
| fan stimulates tnf(alpha)-induced gene expression, leukocyte recruitment, and humoral response. | factor associated with neutral sphingomyelinase activation (fan) is an adaptor protein that constitutively binds to tnf-r1. microarray analysis was performed in fibroblasts derived from wild-type or fan knockout mouse embryos to evaluate the role of fan in tnf-induced gene expression. approximately 70% of tnf-induced genes exhibited lower expression levels in fan-deficient than in wild-type fibroblasts. of particular interest, tnf-induced expression of cytokines/chemokines, such as il-6 and cxcl ... | 2009 | 19786552 |
| sodium chloride affects listeria monocytogenes adhesion to polystyrene and stainless steel by regulating flagella expression. | to study the adhesion capability of seven strains of listeria monocytogenes to polystyrene and stainless steel surfaces after cultivation at various nacl concentrations. | 2009 | 19793195 |
| synergistic antibacterial and anti-inflammatory activity of temporin a and modified temporin b in vivo. | temporins are antimicrobial peptides secreted by the granular glands of the european red frog (rana temporaria). they are 10-14 amino acid long polypeptides active prevalently against gram positive bacteria. this study shows that a synthetic temporin b analogue (tb-yk), acquires the capacity to act in synergism with temporin a and to exert antimicrobial and anti-inflammatory activity in vivo against gram positive and gram negative bacteria. administration of 3.4 mg/kg of temporin a (ta)+1.6 mg/k ... | 2009 | 19784377 |
| therapeutic cancer vaccines in cervical cancer: phase i study of lovaxin-c. | producing effective therapeutic vaccines has proved much more difficult and challenging than developing cancer preventive vaccines. despite huge research in the area of cancer immunology, fda/emea have not approved any type of cancer treatment vaccine so far. more than 99% of cervical cancers have detectable amounts of human papillomavirus (hpv) dna. integration of high-risk hpv into the host cell genome is followed by continual expression of hpv e6 and e7 oncoproteins, making them excellent tar ... | 2009 | 19785060 |
| a molecular beacon-based duplex real-time polymerase chain reaction assay for simultaneous detection of escherichia coli o157:h7 and listeria monocytogenes in milk and milk products. | in this study, a real-time polymerase chain reaction assay based on two specific molecular beacons tagged with different reporter dyes was designed and developed for escherichia coli o157:h7 and listeria monocytogenes in such a way that each pathogen could be detected simultaneously in a single tube and differentiated. the duplex assay was developed by targeting the rfb gene of e. coli o157:h7 and the hly gene of l. monocytogenes using the homemade master reaction mix. the detection limit of the ... | 2009 | 19735201 |
| regulation of mannose phosphotransferase system permease and virulence gene expression in listeria monocytogenes by the eii(t)man transporter. | the eii(t)(man) phosphotransferase system (pts) permease encoded by the mpt operon is the principal glucose transporter in listeria monocytogenes. eii(t)(man) participates in glucose-mediated carbon catabolite repression (ccr) and downregulation of virulence gene expression, and it is the receptor for class iia bacteriocins. the regulation of this important protein and its roles in gene control were examined using derivatives of strain egd-e in which the mpt operon or its regulatory genes, manr ... | 2009 | 19734332 |
| study on the microbial status of unseasoned ground turkey meat from an eu producer-a new product with risk potential? | 1. the aim of this study was to point out potential risks associated with a product new to the market in the european union: unseasoned minced turkey meat. 2. on 6 d of sampling, minced turkey meat from a large-scale eu producer was analysed at 4 processing stages. 3. the packaged minced meat was examined during 10 d of storage, once using the legally recommended conditions (<+2 degrees c), and then under simulated consumer handling; one batch was stored for 3 d at +2 degrees c then kept for 45 ... | 2009 | 19735019 |
| pulsed light inactivation of listeria monocytogenes through different plastic films. | the efficacy of decontamination by pulsed light technology through different plastic films has been assayed using listeria monocytogenes scott a as target microorganism. a 12-mum polyethylene film, a 48-mum polyamide/polyethylene/vinyl acetate-based copolymer, and a 60-mum polyamide/polyethylene copolymer were tested. noble agar plates were surface inoculated and wrapped with different films. unwrapped plates were also analyzed as control. fluences of 0.175 and 0.35 j/cm(2) were applied. pulsed ... | 2009 | 19735198 |
| sting regulates intracellular dna-mediated, type i interferon-dependent innate immunity. | the innate immune system is critical for the early detection of invading pathogens and for initiating cellular host defence countermeasures, which include the production of type i interferon (ifn). however, little is known about how the innate immune system is galvanized to respond to dna-based microbes. here we show that sting (stimulator of interferon genes) is critical for the induction of ifn by non-cpg intracellular dna species produced by various dna pathogens after infection. murine embry ... | 2009 | 19776740 |
| prevalence and level of listeria monocytogenes and other listeria species in selected retail ready-to-eat foods in the united kingdom. | although listeriosis is a rare cause of human disease in the united kingdom, an increase in the number of cases has been observed since 2001, almost exclusively in persons older than 60 years. this increase prompted this study on the microbiological safety of ready-to-eat (rte) foods, which included those types potentially linked to cases of listeriosis. between may 2006 and april 2007, 6,984 rte foods were sampled (2,168 sliced meats, 1,242 hard cheese, 1,088 sandwiches, 878 butter, 725 spreada ... | 2009 | 19777888 |
| safety-based shelf life model for frankfurters based on time to detect listeria monocytogenes with initial inoculum below detection limit. | the growth of listeria monocytogenes inoculated on frankfurters at four inoculum levels (0.1, 0.04, 0.01, and 0.007 cfu/g) was examined at 4, 8, and 12 degrees c until the time l. monocytogenes populations reached a detectable limit of at least 2 cfu/g. a scaled-down assumption was made to simulate a 25-g sample from a 100-lb batch size in a factory setting by using a 0.55-g sample from a 1,000-g batch size in a laboratory. samples of 0.55 g were enriched in pdx-lib selective medium, and presump ... | 2009 | 19777889 |
| effects of mussel processing soils on the adherence of listeria monocytogenes to polypropylene and stainless steel. | a comparative study of adhesion kinetics of l. monocytogenes (strains cect 5873, cect 936, cect 911, and cect 4032, representing serotypes 1/2a, 1/2b, 1/2c, and 4b, respectively) to polypropylene (pp) and stainless steel (ss) under two surface contamination conditions in plants processing cooked mussel was carried out. the conditions were either (i) contamination of clean surfaces with mussel cooking juice carrying l. monocytogenes or (ii) contamination with l. monocytogenes after soiling with m ... | 2009 | 19777890 |
| properties of whey protein-based films containing organic acids and nisin to control listeria monocytogenes. | whey protein isolate and glycerol were mixed to form a matrix to incorporate antimicrobial agents and produce edible films with antimicrobial activity against listeria monocytogenes strains isolated from cheeses. various organic acids were used to decrease ph down to approximately 3. in a preliminary assay without nisin, the effect of each organic acid was evaluated with respect to the rheological properties of the film solutions and the inhibitory and mechanical properties of the films. lactic, ... | 2009 | 19777891 |
| disinfection of iceberg lettuce by titanium dioxide-uv photocatalytic reaction. | securing the physical quality and microbial safety of fresh foods has been a major focus in the food industry. to improve quality and increase the shelf life of fresh produce, disinfection methods have been developed. titanium dioxide (tio2) photocatalytic reactions under uv radiation produce hydroxyl radicals that can be used for disinfection of foodborne pathogenic bacteria. we investigated the effects of tio2-uv photocatalytic disinfection on the shelf life of iceberg lettuce. counts of natur ... | 2009 | 19777894 |
| comparison between the vitek immunodiagnostic assay system and pcr for the detection of pathogenic microorganisms in an experimental dry sausage during its curing process. | the comparison between the vitek immunodiagnostic assay system (vidas) and pcr methods for the detection of the pathogenic microorganisms salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7, clostridium perfringens, and staphylococcus aureus in salchich6n (a type of spanish fermented dry sausage) was studied. the automated mini-vidas method and the pcr method were used to detect the presence of these microorganisms in 90 samples grouped into six batches (one control batch, a ... | 2009 | 19777902 |
| co-infection of market-age turkeys with escherichia coli and listeria monocytogenes in two stress models. | we have hypothesized that stress-induced subclinical infection of turkeys with listeria monocytogenes may be an overlooked source of processing plant contamination, and we have shown that concurrent escherichia coli challenge can increase l. monocytogenes colonization. the objective of this study was to determine the effects of dexamethasone (dex) immunosuppressive treatment and transport stress on the isolation of l. monocytogenes in an e. coli-l. monocytogenes challenge model. thirteen-week-ol ... | 2009 | 20095148 |
| an analysis of the factors for microbial contamination risk for pork at slaughterhouses in korea using the logit model. | to assess the effect of slaughtering practices on the probability of microbial contamination at slaughterhouses in korea, 840 samples from 8 slaughterhouses were collected and 50 factors observed for 2 yr. target microorganisms were salmonella spp. and listeria monocytogenes and 20 contaminated samples were found. twenty-one out of 50 factors were identified as possible sources of microbial contamination. to narrow down the more critical factors and quantify the effects, simple regression analys ... | 2009 | 20077220 |