Publications

[use of different nutrient medium variants in determining the antimicrobial activity of neomycin].	gel diffusion regularities with respect to neomycin sulfate substances differing in their composition and the antibiotic separate components were studied on nutrient media of diverse composition. optimal conditions for standardization of neomycin sulfate antimicrobial activity with using the agar diffusion method and synthetic medium are determined.	1987	3125785
use of the direct epifluorescent filter technique for the enumeration of bacterial spores.	heat treatment at 80 degrees c for 10 min effectively destroyed all vegetative cells (except for gram-positive cocci) and made easier the counting of bacterial spores, which stained orange, green or rarely transparent/black with a dull green halo, in the direct epifluorescent filter technique. the numbers of both orange- or green-staining spores were lower than the plate count. a variety of physiological conditions were used to investigate the relationship of the different staining patterns with ...	1987	3126175
involvement of calcium and dipicolinic acid in the resistance of bacillus cereus bis-59 spores to u.v. and gamma radiations.	the role of dipicolinic acid (dpa) in determining the resistance of bacillus cereus spores to u.v. and gamma radiation was investigated. b. cereus bis-59 spores containing varying amounts of dpa were prepared by appropriate compositional adjustments in the secondary media. compared with spores containing 6 per cent dpa (dry weight) those containing 0.8 per cent dpa were far more sensitive to u.v. radiation. similar u.v. radiation sensitivity was also found in respect of a dpa-less mutant of b. c ...	1987	3100469
the ph dependence of spectral parameters for kalckar's adenosine deaminase assay.	optimal monitor wavelengths and differential millimolar extinction coefficients (m delta epsilon) for rate determination of reactions catalyzed by adenosine deaminases on several substrates have been investigated as a function of ph in the range from 6.5 to 12. the values found are in some cases at variance with those quoted in the biochemical literature. the effect of ph on m delta epsilon values is shown to be clearly related to acid-base properties of product and/or substrate in the reaction. ...	1987	3124669
continuous enzymatically catalyzed production of l-leucine from the corresponding racemic hydroxy acid.		1987	3124688
an x-ray-crystallographic study of beta-lactamase ii from bacillus cereus at 0.35 nm resolution.	crystals of beta-lactamase ii (ec 3.5.2.6., 'penicillinase') from bacillus cereus were grown with cd(ii) in place of the natural zn(ii) cofactor and stabilized by cross-linking with glutaraldehyde. their space group is c2, the cell dimensions are a = 5.44 nm, b = 6.38 nm, c = 7.09 nm and beta = 93.6 degrees, and there is one molecule in the asymmetric unit. diffraction data were collected from cross-linked crystals of the cd(ii)-enzyme, the apoenzyme and six heavy-atom derivatives. the electron- ...	1987	3124808
beta-lactamase i from bacillus cereus. structure and site-directed mutagenesis.	the sequence of the gene for beta-lactamase i from bacillus cereus 569/h has been redetermined. oligonucleotide-directed mutagenesis has been carried out, and the effects of the changes on the ampicillin-resistance of escherichia coli tg1 expressing the mutant genes have been studied. lysine-73, close to the active-site serine-70 and a highly-conserved residue, has been converted into arginine. this change had a large effect on activity, but did not abolish it. an even larger effect was found in ...	1987	3124817
posttraumatic bacillus cereus endophthalmitis.	we encountered a patient who developed bacillus cereus endophthalmitis following trauma. early therapy, which included intravitreal clindamycin phosphate and gentamicin sulfate, resulted in a visual acuity of 20/60. a five-year retrospective review of all cases of endophthalmitis following trauma reported at our institution revealed bacillus as the infecting organism in six (46%) of 13 culture-positive cases. the high frequency of virulent bacillus infections in the setting of trauma necessitate ...	1987	3103589
structural and catalytic properties of l-alanine dehydrogenase from bacillus cereus.	alanine dehydrogenase from bacillus cereus, a non-allosteric enzyme composed of six identical subunits, was purified to homogeneity by chromatography on blue-sepharose and sepharose 6b-cl. like other pyridine-linked dehydrogenases, alanine dehydrogenase is inhibited by cibacron blue, competitively with respect to nadh and noncompetitively with respect to pyruvate. the enzyme was inactivated by 0.1 m glycine/hcl (ph 2) and reactivated by 0.1 m phosphate (ph 8) supplemented with nad+ or nadh. the ...	1987	3104322
endogenous bacillus cereus panophthalmitis.	over the past seven years we have treated three cases of drug abusers in whom endogenous bacillus cereus endophthalmitis rapidly progressed to panophthalmitis. ocular features of infection with this organism include severe pain, chemosis, proptosis, corneal infiltration and ring abscess, subretinal exudation, retinal hemorrhages, and perivasculitis. the process becomes fulminant in an explosive manner and may be accompanied by fever and leukocytosis. ophthalmologists should be cognizant of the a ...	1987	3105407
on the formation of a dicloxacillin-p-hydroxymercuribenzoate suicide complex mediated by beta-lactamase i from bacillus cereus.	p-hydroxymercuribenzoate is a non-competitive inhibitor of beta-lactamase i from bacillus cereus and also, after preliminary preincubation, an inactivator of the enzyme. submitted to the simultaneous action of pcmb plus dicloxacillin, the enzyme completely loses its activity. extensive dialysis can restore the enzymatic activity only if preincubation had been carried out with either pcmb or dicloxacillin but not if both inhibitors had been simultaneously present. mercaptoethanol protects the enz ...	1987	3105535
serious infections caused by bacillus species.	thirty-eight patients with serious infections caused by organisms belonging to the genus bacillus are described. our experience, and that reported in the literature, indicates that, in most cases, isolated bacillus bacteremia is not a particularly serious disease. therefore, under most circumstances, empiric antibiotic therapy designed specifically for treatment of bacillus is probably not necessary. endocarditis can occur, but apparently follows bacteremia only infrequently. when these bacteria ...	1987	3106749
factors influencing the activity of cellular alkaline phosphatase during growth and sporulation of bacillus cereus.	alkaline phosphatase (ec 3.1.3.1) is synthesized in media with a low phosphate concentration (0.37 mm of total and 19 microm of inorganic phosphate, respectively) already during the exponential phase of growth of bacillus cereus. the enzyme is repressed by higher phosphate concentrations (3.7 mm) during the whole growth period; during sporogenesis the enzyme activity in cells slightly increases even under these conditions. during growth the enzyme is not secreted into the medium, a minor amount ...	1987	3108119
[experimental basis of principles for creating microbiologic standards for food products for children during the 1st year of life].	standard media were designed simulating the gastro-intestinal contents of infants during the first year of life. these media were used in the in vitro tests to study the viability of a number of potentially-pathogenic microorganisms. it was shown that s. aureus, e. coli o-iii, s. liquefaciens not only survived but also multiplied in the media with ph values similar to those of gastric juice in infants of the first year of life, in the presence of the mixture for child nutrition "malysh". the res ...	1987	3109120
microincineration and elemental x-ray microanalysis of single bacillus cereus t spores.	single whole spores of bacillus cereus t were analyzed by scanning electron microscopy and electron microprobe x-ray microanalysis before and after high-temperature (600 degrees c) ashing in air. high-temperature ashing consisted of a centripetal oxidation of the spore surface combined with pyrolysis of the spore's interior. ashing of single spores produced a compact central ash particle, mimicking the much larger unashed spore body in outline but containing craterlike microregions, and a periph ...	1987	3109722
new trends in enzyme recovery.		1987	3111333
bacillus cereus phospholipase c: carboxylic acid ester specificity and stereoselectivity.	thiophosphate analogs of phosphatidylcholine have been synthesized with varying structural complexity. these analogs have been used in a continuous spectrophotometric assay for phospholipase c (bacillus cereus) to estimate the minimal structural requirements associated with the non-polar portion of the substrate phospholipid. the analogs were of three types containing zero, one or two carboxylic acid ester functionalities. the analogs with one or two ester groups acted as substrates for phosphol ...	1987	3111540
bacillus cereus bacteremia in an intravenous drug abuser.		1987	3111782
disguise of antibiotics.		1987	3112587
effect of thermal treatments in oils on bacterial spore survival.	the heat resistance of bacillus cereus f4165/75, clostridium sporogenes pa 3679 and cl. botulinum 62a spores suspended in buffer (ph 7.2), olive oil and a commercial oil (a mixture of rapeseed oil and soy oil) was investigated. linear survivor curves were obtained with b. cereus spores in the three menstrua and with 62a and pa 3679 spores suspended in buffer. however, the inactivation kinetics of the clostridial spores suspended in oils were concave upward with a characteristic tailing-off for 6 ...	1987	3114210
tailing of survivor curves of clostridial spores heated in edible oils.	tailing of survivor curves was observed for clostridium sporogenes pa 3679 and cl. botulinum 62a spores heated whilst suspended in edible oils, but not for the same spores suspended in buffer (ph 7.2) or mineral oil or for bacillus cereus f4165/75 spores suspended in buffer or oils. the tailing cannot be ascribed to a genetic or developmental heterogeneity in the resistance of the spore population or to a heterogeneity of the treatment severity during heating. heat adaptation due to the release ...	1987	3114211
[isolation and characterization of auxotrophic and antibiotic-resistant mutants of bacillus cereus].		1987	3114855
kinetics and mechanism of the serine beta-lactamase catalyzed hydrolysis of depsipeptides.	steady-state kinetic parameters have been determined for the hydrolysis of a series of acyclic depsipeptides (ester analogues of acyl-d-alanyl-d-alanine peptides) catalyzed by representative class c (enterobacter cloacae p99) and class a (bacillus cereus i, tem-2, and staphylococcus aureus pc1) beta-lactamases. the best of these substrates, and the one most used in this work, was m-[[(phenylacetyl)-glycyl]oxy]benzoic acid, whose rates of cleavage could be followed spectrophotometrically. the p99 ...	1987	3115289
oxygen profiles in, and in the agar beneath, colonies of bacillus cereus, staphylococcus albus and escherichia coli.	the paper reports the use of microelectrodes to measure o2 penetration in different aged colonies of bacillus cereus, escherichia coli and staphylococcus albus. in young (18 h) colonies of b. cereus and e. coli o2 disappeared at depths of 25-30 micron and 35-40 micron respectively. in young s. albus colonies, o2 reached a minimum but was never completely absent. as colonies aged (24-168 h) the depth to which o2 penetrated increased.	1987	3116170
pathogenic role of bacillus cereus in wound infections in the tropics.	a bacteriological survey was undertaken on clinically infected traumatic wounds amongst a group of young and fit operation raleigh members, who were living and working in a remote area of costa rican rain forest. all infected wounds were swabbed before treatment and, where possible, at intervals during treatment. swabs were also obtained from the nose and throat of each patient. all swabs were stored by desiccation in sterile silica gel for culture at a later date. culture revealed a high rate o ...	1987	3116240
traumatic wound infection due to bacillus cereus in an immunocompromised patient: a case report.	a young man recently responding to immunosuppressive therapy for acute myelocytic leukemia was admitted with fever and haemorrhagic blebs on both extremities after sustaining some scratch marks in a muddy pond. gram stains of the hemorrhagic fluid in the blebs revealed many gram positive bacilli. b. cereus was identified from culture of tissue fluid. he did not respond to therapy despite bacteriological cure. terminally, he developed pseudomonas aeruginosa bacteremia and generalized bleeding.	1987	3116676
[aerosol disinfection of bacterial spores].	the present investigations are divided into two parts. first it is tested which commercial disinfectants are efficient in aerosol disinfection of bacterial spores. this part is carried out in an aerosol chamber with airborne spores (laboratory experiments). the best results are obtained with peracetic acid, hydrogen peroxide and formaldehyde are effective with some restrictions. with these disinfectants it is tested in the second part if the aerosol disinfecting-method is capable for disinfectin ...	1987	3116785
kinetic and structural characterization of reversibly inactivated beta-lactamase.	the reversible inhibition of beta-lactamase i from bacillus cereus by cloxacillin, methicillin, and nafcillin has been systematically investigated. for these substrates the enzymatic reaction involves partitioning of the substrate between turnover and inhibition. typically, concentrations of several hundred millimolar are necessary for complete inactivation. the completely inactivated enzyme could be formed by incubation at temperatures above 20 degrees c, where inhibition competes more effectiv ...	1987	3117100
identification of self-transmissible plasmids in four bacillus thuringiensis subspecies.	the transfer of plasmids by mating from four bacillus thuringiensis subspecies to bacillus anthracis and bacillus cereus recipients was monitored by selecting transcipients which acquired plasmid pbc16 (tcr). transcipients also inherited a specific large plasmid from each b. thuringiensis donor at a high frequency along with a random array of smaller plasmids. the large plasmids (ca. 50 to 120 megadaltons), pxo13, pxo14, pxo15, and pxo16, originating from b. thuringiensis subsp. morrisoni, b. th ...	1987	3117773
effect of auranofin and other gold complexes on the activity of phospholipase c.	auranofin (af) is an orally active chrysotherapeutic agent used for the treatment of rheumatoid arthritis, a self-perpetuating inflammatory disease. because of reports suggesting that af and other gold complexes can, under certain circumstances, exacerbate rheumatoid inflammatory lesions in humans and adjuvant arthritic rats and that phospholipase c (plc) and phospholipase a2 activities are increased in rheumatoid patients, the effects of af and a related gold complex on in situ mammalian and pu ...	1987	3118179
[molecular nature of the pathogenic effect induced by b. cereus].	the paper shows that biological properties of the dl-toxin are determined by structural particularities of this substance. it is supposed that the component b with mol. mass of 42,000 performs the ligand function enabling fixation of the toxin on a target cell. the component a with mol. mass of 37,000 has been characterized as an activator in reproducing oedematous and diarrheagenic effect. the component c activates the induction of the lethal effect. the optimal ratios of these components neede ...	1987	3118202
n-pyrrylderivatives of penicillins. kinetics and inhibition studies with clavulanic acid of a bacillus cereus beta-lactamase.		1987	3118398
a continuous spectrophotometric assay for the bacillus cereus phospholipase c using a thiophosphate substrate analog: evaluation of assay conditions and chromogenic agents.	a thiophosphate analog of dioctanoylphosphatidylcholine has been used as the substrate in a continuous spectrophotometric assay for the bacillus cereus phospholipase c. the reaction has been monitored at 412 nm using 5,5'-dithiobis(2-nitrobenzoic acid) (dtnb) and at 324 nm using 4,4'-dithiopuridine (dtp) as the respective thiol-reactive chromogenic agents. an optimum ph 6.0 was determined for the phospholipase c-catalyzed reaction which was independent of the chromogen utilized. although the rea ...	1987	3118733
antimicrobial activity of lysozyme against bacteria involved in food spoilage and food-borne disease.	egg white lysozyme was demonstrated to have antibacterial activity against organisms of concern in food safety, including listeria monocytogenes and certain strains of clostridium botulinum. we also found that the food spoilage thermophile clostridium thermosaccharolyticum was highly susceptible to lysozyme and confirmed that the spoilage organisms bacillus stearothermophilus and clostridium tyrobutyricum were also extremely sensitive. several gram-positive and gram-negative pathogens isolated f ...	1987	3118808
cryoenzymology of beta-lactamases.	the cryoenzymology of several different beta-lactamases has been investigated. particular attention has been paid to the experimental pitfalls of the technique. these include such factors as false bursts at the start of the reaction, instability of the enzymes during turnover, and km values so high that little of the enzyme is present as a complex. many of the difficulties in cryoenzymology stem from the use of organic cryosolvents. a novel "salt" cryosolvent has been tested: ammonium acetate so ...	1987	3118942
kinetics and mechanism of inactivation of the rtem-2 beta-lactamase by phenylpropynal. identification of the characteristic chromophore.	beta-lactamases of all three classes, a, b, and c, are inactivated by phenylpropynal and p-nitrophenylpropynal. the inactivation of rtem-2 beta-lactamase and of bacillus cereus beta-lactamase i is accelerated in the presence of a type substrates such as dicloxacillin, quinacillin, and cefoxitin, which are thought to expand or loosen the conformation of these enzymes. in the presence and absence of cefoxitin the inactivation of the rtem-2 beta-lactamase is first and second order, respectively, in ...	1987	3119590
camp like reactions for presumptive identification of bacillus cereus from bovines.		1987	3120441
the analysis of enzyme progress curves by numerical differentiation, including competitive product inhibition and enzyme reactivation.	a new method for analyzing steady-state enzyme kinetic data is presented. the technique, which is based on the numerical differentiation of the complete reaction curve, has several advantages over initial velocity and integrated michaelis-menten equation methods. the differentiated data are fit to the differential equation describing the appropriate kinetic scheme. this approach is particularly valuable in cases of strong competitive product inhibition and of changing concentrations of active en ...	1987	3120622
toxigenic bacillus cereus as a cause of wound infections in the tropics.	a bacteriological survey was conducted on clinically infected traumatic wounds in members of an operation raleigh expedition, who were working in the costa rican rain forest. bacillus cereus was isolated from the wounds of 14 of 18 patients, usually in pure and heavy growth. most of the isolates were strongly toxigenic by in vivo pathogenicity tests. the organism was also isolated from the nose in 15 cases and the throat in five cases. the findings indicate that b. cereus was the principle patho ...	1987	3121755
[enterocolitis as a hospital infection of neonates caused by food contaminated with b. cereus].		1987	3121965
[the role of molecular oxygen and energy metabolism in the onset of bacillus cereus spore germination].	bacillus cereus 96 spore germination was shown to depend on the content of molecular oxygen in the growth medium. when oxygen was removed from the medium, the spores germinated 50 min later as compared with this process under aerobic conditions. likewise, spore initiation was delayed by 50 min in a growth medium containing oxygen in quantities optimal for respiration if 100mm kcn was added to it. the spores did not germinate when they had been treated simultaneously with glycolysis and respirati ...	1987	3121988
production of staphylococcal enterotoxin in mixed cultures.	two staphylococcus aureus strains were grown in brain-heart infusion (bhi) broth and a meat medium with bacillus cereus, streptococcus faecalis, escherichia coli, and pseudomonas aeruginosa. both s. aureus strains grew well and produced enterotoxin in the presence of s. faecalis in bhi broth; however, enterotoxin production was observable in the meat medium only when the s. aureus inoculum was greater than the s. faecalis inoculum. s. aureus fri-100 grown with b. cereus produced enterotoxin in b ...	1987	3122653
phosphorylase-mediated mobilization of the amino group of adenine in bacillus cereus.	mobilization of the ribose moiety of purine nucleosides as well as of the amino group of adenine may be realized in bacillus cereus by the concerted action of three enzymes: adenosine phosphorylase, adenosine deaminase, and purine nucleoside phosphorylase. in this pathway, ribose-1-phosphate and inorganic phosphate act catalytically, being continuously regenerated by purine nucleoside phosphorylase and adenosine phosphorylase, respectively. as a result of such a metabolic pathway, adenine is qua ...	1987	3122663
enzymatic hydrolysis of short-chain lecithin/long-chain phospholipid unilamellar vesicles: sensitivity of phospholipases to matrix phase state.	short-chain lecithin/long-chain phospholipid unilamellar vesicles (sluvs), unlike pure long-chain lecithin vesicles, are excellent substrates for water-soluble phospholipases. hemolysis assays show that greater than 99.5% of the short-chain lecithin is partitioned in the bilayer. in these binary component vesicles, the short-chain species is the preferred substrate, while the long-chain phospholipid can be treated as an inhibitor (phospholipase c) or poor substrate (phospholipase a2). for phosph ...	1987	3122829
inactivation of blasticidin s by bacillus cereus. i. inactivation mechanism.		1987	3123450
[plasmid transformation of bacillus cereus protoplasts].	the process of polyethyleneglycol-induced plasmid transformation of bacillus cereus protoplasts was studied. plasmid transfer into bacillus cereus strains was demonstrated with the frequencies 1.3.10(1)-1.6.10(2) transformants per 1 mkg of plasmid dna. the plasmids transferred are stably inherited by bacillus cereus cells causing tetracycline resistance (pbc16) or kanamycin resistance (pub110 and pbd64). the proposed method can be used for construction of bacillus cereus strains having the plasm ...	1987	3123921
increased arachidonic acid metabolites from cells in culture after treatment with the phosphatidylcholine-hydrolyzing phospholipase c from bacillus cereus.	treatment of rat liver cells (the c-9 cell line), porcine aorta endothelial cells, bovine aorta smooth muscle cells, bovine aorta endothelial cells, mouse fibroblasts and rat keratinocytes with highly purified, crystallized bacillus cereus phospholipase c, which hydrolyzes phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine but has little or no effect on phosphatidylinositol, phosphatidylglycerol, cardiolipin, sphingomyelin, lysophosphatidylcholine or lysophosphatidylethanolamin ...	1987	3124216
[bacillus cereus septicemia following intravenous chemotherapy with an implantable chamber].		1987	2962179
[a genetic observation on a resistant variation of bacillus cereus 756 to the new antibiotic 2252].		1987	2964935
improvement of beta-amylase production by new beta-amylase producers and new culture conditions.		1987	2449113
comparative 16s rrna oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives.	the phylogenetic incoherency of the genus bacillus as presently described is demonstrated by analysis of both published and new data from comparative 16s rrna cataloguing of nine bacillus species and a number of related non-bacillus taxa, i.e. caryophanon latum, filibacter limicola and planococcus citreus. while the ellipsoidal-spore-forming bacilli, e.g. b. subtilis and allied species, formed a coherent cluster, the round-spore-forming bacilli showed a higher degree of relationship to the non-s ...	1987	2452227
independent bindings of mn2+ and mg2+ to the active site of b. cereus glutamine synthetase.	glutamine synthetase purified from bacillus cereus ifo 3131 was modified by iodoacetamide and the atp analog 5'-p-fluorosulfonylbenzoyladenosine (fsba). only mg2+-dependent activity was inactivated by iodoacetamide, whereas only mn2+-dependent activity was inactivated by fsba. when iodoacetamide-treated enzyme was reacted with fsba, mn2+-dependent activity was also inactivated. mg2+ plus mn2+-dependent activity was inactivated in any case. the results suggested that the binding sites of mn2+ and ...	1987	2880587
synthesis of a thiophosphate analog of dioctanoylphosphatidylcholine: a phospholipase c substrate.	dioctanoylthiophosphatidylcholine, a racemic thiophosphate analog of l-alpha-dioctanoylphosphatidylcholine, has been synthesized and isolated by flash chromatography. in contrast with the didecanoylthiophosphatidylcholine synthesized previously, the analog is easily dispersed on sonication in aqueous media and is rapidly hydrolyzed to produce a free thiol group in the presence of the extracellular phospholipase c from either bacillus cereus or clostridium perfringens. when 5,5'-dithiobis (2-nitr ...	1987	2889786
phospholipase c and phosphatidylinositol phospholipase c.		1987	3026688
cloning and sequencing of the blaz gene encoding beta-lactamase iii, a lipoprotein of bacillus cereus 569/h.	it has not been clear whether the membrane-bound beta-lactamase iii of bacillus cereus 569 is a separate enzyme or a modified form of the secreted beta-lactamase i. the membrane enzyme is an acyl-glyceride thioether-linked lipoprotein (j. b. k. nielsen and j. o. lampen, biochemistry 22:4652-4656, 1983) and thus is probably a separate entity. we cloned the beta-lactamase iii gene (blaz) on a 4.9-kilobase-pair clai fragment from mutant strain 569/h (constitutive for high-level production of beta-l ...	1987	3027036
tetrameric alkaline phosphatase from human liver is converted to dimers by phosphatidylinositol phospholipase c.	membrane-bound human liver alkaline phosphatase solubilized by a non-ionic detergent, nonidet p-40 (np-40), has the molecular mass of a tetramer. it can be converted to a dimeric form by treatment with a phosphatidylinositol phospholipase c (pi-plc) obtained from bacillus cereus. when human liver plasma membranes were directly treated with pi-plc, the released alkaline phosphatase was dimeric. thus, phosphatidylinositol may help maintain the tetrameric quaternary structure of alkaline phosphatas ...	1987	3028865
the action of sphingomyelinase from bacillus cereus on atp-depleted bovine erythrocyte membranes and different lipid composition of liposomes.	the presence of cholesterol or phosphatidylethanolamine in sphingomyelin liposomes enhanced 2- to 10-fold the breakdown of sphingomyelin by sphingomyelinase from bacillus cereus. on the other hand, the presence of phosphatidylcholine was either without effect or slightly stimulative at a higher molar ratio of phosphatidylcholine to sphingomyelin (3/1). in the bovine erythrocytes and their ghosts, the increase by 40-50% or the decrease by 10-23% in membranous cholesterol brought about acceleratio ...	1987	3036001
cloning and nucleotide sequencing of genes for a second type of small, acid-soluble spore proteins of bacillus cereus, bacillus stearothermophilus, and "thermoactinomyces thalpophilus".	the nucleotide sequences of the single genes coding for the b-type small, acid-soluble spore proteins (sasp) of bacillus cereus, b. stearothermophilus, and "thermoactinomyces thalpophilus" were determined, and the amino acid sequences of all b-type sasp were compared. while this type of sasp showed significant sequence conservation around the two spore protease cleavage sites, alignment of these sequences required the introduction of gaps, and even then only 19 of the residues were conserved exa ...	1987	3036769
effects of phospholipases c from bacteria on binding of enkephalin to rat brain membranes.	the effects of phospholipases c on the equilibrium constants and maximal binding capacities of tritiated [d-ala2,-d-leu5] enkephalin to rat brain membranes were investigated using phosphatidylcholine-hydrolyzing phospholipase c and sphingomyelinase c of bacillus cereus and, phosphatidylinositol-specific phospholipase c of bacillus thuringiensis. when 72% of the phosphatidylinositol in the rat brain membranes was hydrolyzed by phosphatidylinositol-specific phospholipase c, the affinity of opiate ...	1987	3039692
[synthesis of phosphonate analogs of sphingomyelins and preparation of an affinity sorbent for sphingomyelinase purification].	phosphonate analogues of 2-n-stearoyl- (i) and 2-n-(undec-10-enoyl)-sphingomyelins (ii) have been synthesised. compound (ii) was used as a starting product for preparation of a sorbent for sphingomyelinase affinity chromatography. the double bond of the unsaturated undec-10-enoyl moiety of the phosphonate analogue (ii) was oxidized, and the modified (ii) was coupled to amino-toyopearl hw-65 to give a sorbent containing 4 mumoles of ligand per milliliter of the swollen resin.	1987	3040014
purification and properties of an extracellular collagenolytic protease produced by the human oral bacterium bacillus cereus (strain soc 67).	the major collagenolytic proteinase present in the culture filtrate of bacillus cereus (strain soc 67, isolated from the human oral cavity) has been purified to homogeneity by a procedure that comprised concentration of ultrafiltered growth medium on a millipore pttk00005 membrane, precipitation with ammonium sulfate, gel permeation chromatography, chromatofocusing, fast protein liquid chromatography on an anion-exchange column, and finally fast protein liquid chromatography on a gel column. the ...	1987	3040751
bacillus cereus panophthalmitis manifesting as an orbital cellulitis.		1987	3099579
bacillus cereus endophthalmitis.	the authors present a case of a young man with post-traumatic endophthalmitis caused by bacillus cereus. the clinical course was typical of the panophthalmitis caused by this toxin-producing organism: rapid onset of signs of systemic infection, a corneal ring abscess and eventual loss of the globe requiring enucleation. studies of experimental rabbit models of the infection have indicated that the most efficacious regimen consists of systemic and topical clindamycin and gentamicin. successful th ...	1987	3102030
nucleotide sequence of the beta-lactamase i gene of bacillus cereus strains 569/h and 5/b.		1987	3103105
posttraumatic endophthalmitis: the emerging role of bacillus cereus infection.	endophthalmitis resulting from nonsurgical penetrating trauma to the eye is a relatively uncommon infection in the united states. data are limited, but most recently published series have attributed the highest incidence of infection to gram-positive organisms, in particular staphylococcus epidermidis. fungal causes have been reported far less frequently. bacillus species are being recognized increasingly as major causes of posttraumatic ocular disease, with rates of infection often making them ...	1987	3103191
involvement of the spore coat in germination of bacillus cereus t spores.	bacillus cereus t spores were prepared on fortified nutrient agar, and the spore coat and outer membrane were extracted by 0.5% sodium dodecyl sulfate-100 mm dithiothreitol in 0.1 m sodium chloride (sds-dtt) at ph 10.5 (coat-defective spores). coat-defective spores in l-alanine plus adenosine germinated slowly and to a lesser extent than spores not treated with sds-dtt, as determined by decrease in absorbance and release of dipicolinic acid and ca2+. spores germinated in calcium dipicolinate onl ...	1987	3103533
modification of membrane sulfhydryl groups in bacteriostatic action of nitrite.	the mechanism by which nitrite inhibits outgrowing spores of bacillus cereus t was examined by using techniques developed earlier for nitrite analogs. the morphological stage of inhibition, cooperativity effects, effect of ph on inhibition, kinetics of protection against iodoacetate incorporation into membrane sulfhydryl groups, and protection against the bacteriocidal effect of carboxymethylation by iodoacetate indicate that nitrite acts as a membrane-directed sulfhydryl agent. the mechanism by ...	1987	3103535
a new bioassay system for screening high berberine-producing cell colonies of thalictrum minus.	a new method of estimating the amount of berberine released from minute cell colonies of thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. in this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and the concentration of berberine which has been released from the cells into the agar piece is assayed by the antibacterial activity against bacillus cereus mt2026. screening of 1000 ...	1987	24248650
acetyl-coenzyme a: arylamine n-acetyltransferases in microorganisms: screening and isolation of an enzyme from bacillus cereus.	the raw extracts of a series of microorganisms were screened for the presence of acetyl-coenzyme a: arylamine n-acetyltransferase (aaat) using a radioactive assay with 3h-acetyl-coenzyme a and aniline as substrates. enzyme activities were primarily detected in the soluble fractions of bacillus and nocardia species, and in some further soil organisms. only strains of bacillus cereus were able to acetylate 4-nitroaniline and 3,5-dimethyl-4-nitroaniline. the fermentation conditions for the producti ...	1986	3103579
changes in the coordination geometry of the active-site metal during catalysis of benzylpenicillin hydrolysis by bacillus cereus beta-lactamase ii.	rapid-scanning stopped-flow spectroscopy (425-700 nm) has been used to study spectral changes in cobalt(ii)-substituted bacillus cereus beta-lactamase ii during the binding and hydrolysis of benzylpenicillin. the experiments were carried out in aqueous solution over a temperature range of 3-20 degrees c. three metallointermediates have been characterized by their visible absorption spectra. two of them have visible absorption spectra identical with the intermediates es1 and es2 previously observ ...	1986	3099831
effect of phospholipases on chronic opiate action in neuroblastoma x glioma ng108-15 hybrid cells.	chronic treatment of neuroblastoma x glioma ng108-15 hybrid cells with opiate agonist resulted in loss of the acute opiate inhibition of adenylate cyclase activity with a concomitant increase in the enzymatic activity observable on addition of the antagonist naloxone. the role of membrane lipids in the cellular expression of these chronic opiate effects was investigated by the hydrolysis of phospholipids with various lipases. treatment with phospholipase c from clostridium welchii produced an en ...	1986	3018158
effects of metal ions on sphingomyelinase activity of bacillus cereus.	some divalent metal ions were examined for their effects on sphingomyelinase activity of bacillus cereus. the enzyme activity toward mixed micelles of sphingomyelin and triton x-100 proved to be stimulated by co2+ and mn2+, as well as by mg2+. km's for co2+ and mn2+ were 7.4 and 1.7 microm, respectively, being smaller than the km for mg2+ (38 microm). sr2+ proved to be a competitive inhibitor against mg2+, with a ki value of 1 mm. zn2+ completely abolished the enzyme activity at concentrations a ...	1986	3019244
a spectral study of cobalt(ii)-substituted bacillus cereus phospholipase c.	the coordination sphere of both the structural and catalytic zinc ions of bacillus cereus phospholipase c has been probed by substitution of cobalt(ii) for zinc and investigation of the resultant derivatives by a variety of spectroscopic techniques. the electronic absorption, circular dichroic, magnetic circular dichroic, and electron paramagnetic resonance spectra were found to be strikingly similar when cobalt(ii) was substituted into either site and are consistent with a distorted octahedral ...	1986	3019384
phospholipid requirement of ca2+-stimulated, mg2+-dependent atp hydrolysis in rat brain synaptic membranes.	the phospholipid requirement for ca2+-stimulated, mg2+-dependent atp hydrolysis (ca2+/mg2+-atpase) and mg2+-stimulated atp hydrolysis (mg2+-atpase) in rat brain synaptosomal membranes was studied employing partial delipidation of the membranes with phospholipase a2 (hog pancreas), phospholipase c (bacillus cereus) and phospholipase d (cabbage). treatment with phospholipase a2 caused an increase in the activities of both ca2+/mg2+-atpase and mg2+-atpase whereas with phospholipase c treatment both ...	1986	2946970
studies on glucosyltransferase and endogenous glucosyl acceptor in bacillus cereus ahu 1030 membranes.	a glucosyltransferase, extracted from the membranes of bacillus cereus ahu 1030 with tris-hcl buffer containing 0.1% triton x-100 at ph 9.5, was separated from an endogenous glucosyl acceptor by chromatography on deae-sepharose cl-6b subsequent to chromatography on sepharose 6b. structural analysis data showed that the glucosyl acceptor was a glycerol phosphate polymer linked to beta-gentiobiosyl diglyceride. the enzyme catalyzed the transfer of glucosyl residues from udp-glucose to c-2 of the g ...	1986	2952639
experimental bacillus cereus mastitis in cows.		1986	2953188
two different parasporal inclusions are produced by bacillus thuringiensis subsp. finitimus.	bacillus thuringiensis subsp. finitimus produced at least two parasporal inclusions. one inclusion was formed within the exosporium and remained with the spore after mother cell lysis. a second inclusion formed somewhat later exterior to the exosporium. each inclusion contained a major polypeptide of about 135,000 daltons with unique antigenic determinants. this subspecies contained only two plasmids, of 98 and 77 megadaltons (mda). strains cured of these plasmids produced only the free inclusio ...	1986	3001026
cellular and humoral defenses of glossina (diptera: glossinidae): reactions against bacteria, trypanosomes, and experimental implants.		1986	3081723
on the origin of bacterial resistance to penicillin: comparison of a beta-lactamase and a penicillin target.	structural data are now available for comparing a penicillin target enzyme, the d-alanyl-d-alanine-peptidase from streptomyces r61, with a penicillin-hydrolyzing enzyme, the beta-lactamase from bacillus licheniformis 749/c. although the two enzymes have distinct catalytic properties and lack relatedness in their overall amino acid sequences except near the active-site serine, the significant similarity found by x-ray crystallography in the spatial arrangement of the elements of secondary structu ...	1986	3082007
biosynthesis of the wall acidic polysaccharide in bacillus cereus ahu 1356.	biosynthetic studies on an acidic polysaccharide, comprising galactose, rhamnose, n-acetylglucosamine and sn-glycerol 1-phosphate, were carried out with a membrane system obtained from bacillus cereus ahu 1356. incubation of the membranes with udp-[14c]gal, tdp-[14c]rha and udp-[14c]glcnac resulted in the formation of four or more labeled-sugar-linked lipids and a labeled polysaccharide. data on structural analysis of the sugar moieties released from the glycolipids, together with results of enz ...	1986	3082626
inactivation of bacillus spores by gaseous ozone.	the sporicidal activity of ozone in the gas phase was investigated. spores of six strains of bacillus species deposited on filter paper or glass fibre filter were conditioned at different relative humidities (r.h.), and then exposed to ozone ranging in concentration from 0.5 to 3.0 mg/l at different r.h. there was a lag phase in the initial stage of exposure followed by an exponential decrease in the number of survivors with time, although no lag phase was observed with one strain. inactivation ...	1986	3082844
[post-traumatic endophthalmitis caused by bacillus cereus].	a young man with severe endophthalmitis caused by b. cereus is described following a trauma with an intraocular foreign body. although this organism was isolated from the aqueous with 18 hours and specific antibiotic therapy instituted, the eye subsequently lost all function and had to be enucleated because of severe pain. an extremely rapidly developing corneal ring abscess seems to be the hallmark of an intraocular infection with b. cereus organisms.	1986	3083151
plaster-associated bacillus cereus wound infection. a case report.	plaster of paris and nonsterile cast padding have previously been implicated in wound infections. the observation of three pin site infections with bacillus cereus following pin placement and plaster fixation of an open forearm fracture led to an epidemiologic investigation. two hundred sixty-one bacterial cultures were taken from materials in the steinmann pin application and plaster fixation procedures. b. cereus with a similar antibiogram and biotype as was identified in the pin site cultures ...	1986	3083406
germination of bacillus cereus spores induced by purine ribosides and their analogs: effects of modification of base and sugar moieties of purine nucleosides on germination-inducing activity.	purine riboside and some of its analogs were tested for their ability to induce germination of bacillus cereus t spores. hypoxanthine and adenine showed no germination-inducing activity either in the present or absence of d-ribose or its phosphorylated derivatives. purine riboside and 18 analogs with modified purine base were all able to induce germination of the spores to various extents. in contrast to this, the requirement for the sugar moiety in the purine riboside appeared to be more string ...	1986	3084054
bacillus cereus: a snake in the grass for granulocytopenic patients.		1986	3084672
single-stranded plasmid dna in bacillus subtilis and staphylococcus aureus.	plasmid pc194 was found to exist in a double-stranded and a single-stranded dna form in bacillus subtilis and staphylococcus aureus. this single-stranded dna was found as a circular molecule of the same size as the parental monomer and corresponded to only one of the two dna strands. it represented one-third of plasmid copies. single- and double-stranded dna copies in similar proportions to the above were detected for five other s. aureus plasmids (pc221, pc223, pe194, pt127, and pt181) and one ...	1986	3085097
[isolation and typing of bacillus cereus phages].		1986	3087713
induced release of bacillus spores from sporangia by sodium sulphate.	incubation of sporulating cultures of bacillus anthracis, b. cereus, b. subtilis and b. thuringiensis in 1.0 mol/l sodium sulphate markedly increased the release of free spores from sporangia. it is postulated that the release of spores is due to activation of latent autolysins which hydrolyse sporangial cell walls. sodium sulphate-induced lysis of sporangia represents a novel and highly effective method for the recovery of spores from cultures of bacillus species.	1986	3087932
cloning and nucleotide sequencing of genes for small, acid-soluble spore proteins of bacillus cereus, bacillus stearothermophilus, and "thermoactinomyces thalpophilus".	as found previously with other bacillus species, spores of b. stearothermophilus and "thermoactinomyces thalpophilus" contained significant levels of small, acid-soluble spore proteins (sasp) which were rapidly degraded during spore germination and which reacted with antibodies raised against b. megaterium sasp. genes coding for a b. stearothermophilus and a "t. thalpophilus" sasp as well as for two b. cereus sasp were cloned, their nucleotide sequences were determined, and the amino acid sequen ...	1986	3087949
laser raman spectroscopy of lyophilized bacterial spores.	laser-excited raman spectra were examined in lyophilized spores of bacillus cereus. in a comparison of the spectrum of the dormant spore with that of the germinated spore, we found several raman bands which occurred in the former but not in the latter. among these raman bands, the 1,573, 1,395, 1,017, 822, and 662 cm-1 bands were assigned to the vibrational frequencies of calcium dipicolinate (cadpa). no raman bands and peaks due to dipicolinic acid (h2dpa) were observed. this raman evidence ind ...	1986	3088398
bacillus cereus--maine.		1986	3088419
purification and characterization of bacillus coagulans oligo-1,6-glucosidase.	a p-nitrophenyl-alpha-d-glucopyranoside-hydrolyzing oligo-1,6-glucosidase of bacillus coagulans atcc 7050 (facultative thermophile) was purified to homogeneity. the relative molecular mass, stokes radius, sedimentation coefficient at 20 degrees c in water, molecular absorption coefficient at 280 nm and ph 6.8, and isoelectric point were estimated as 60 000, 3.29 nm, 4.8 x 10(-13) s, 1.34 x 10(5) m-1 cm-1, and 4.3, respectively. the amino-terminal amino acid was threonine. there was no common ant ...	1986	3089779
plipastatins: new inhibitors of phospholipase a2, produced by bacillus cereus bmg302-ff67. i. taxonomy, production, isolation and preliminary characterization.	plipastatins have been isolated as part of a program designed to find inhibitors of porcine pancreatic phospholipase a2. they were purified from fermentation broth of bacillus cereus bmg302-ff67 and finally separated into four fractions by reverse phase hplc. the respective fractions were designated as plipastatins a1, a2, b1 and b2. plipastatins also inhibited phospholipases c and d.	1986	3089997
plipastatins: new inhibitors of phospholipase a2, produced by bacillus cereus bmg302-ff67. ii. structure of fatty acid residue and amino acid sequence.	plipastatins, new inhibitors of phospholipase a2, were produced by a strain classified as bacillus cereus bmg302-ff67. the plipastatins are a family of acylated decapeptides which differ from each other by amino acid composition and the nature of the fatty acid side chain. the fatty acids have been shown to be 3(r)-hydroxyhexadecanoic acid (n-c16h3) and 14(s)-methyl-3(r)-hydroxyhexadecanoic acid (a-c16h3) by mass, nmr and optical rotation. amino acid sequence analysis by secondary ion mass spect ...	1986	3089998
plipastatins: new inhibitors of phospholipase a2, produced by bacillus cereus bmg302-ff67. iii. structural elucidation of plipastatins.	plipastatins are new inhibitors of phospholipase a2 produced by bacillus cereus bmg302-ff67. structures of the plipastatins have been determined by uv, mass and nmr spectrometries and chemical degradation. the carboxyl group of the c-terminal l-isoleucine of plipastatinic acid has been shown to form a lactone linkage with the hydroxyl group of l-tyrosine. the total structure of plipastatins has thus been established.	1986	3089999
respiratory systems of the bacillus cereus mother cell and forespore.	the respiratory systems of the mother cells and forespores of bacillus cereus were compared throughout the maturation stages (iii to vi) of sporulation. the results indicated that both cell compartments contain the same assortment of oxidoreductases and cytochromes. however membrane fractions from young forespores were clearly distinct from those of the mother cell, i.e., lower content of cytochrome aa3, lower cytochrome c oxidase activity, higher concentration of cytochrome o, and a lower sensi ...	1986	3090018
a new form of coagulation factor vii in plasma.	we have reported the existence of a novel form of coagulation factor vii - probably a factor vii-phospholipid complex - in plasma from pregnant women and men at risk for cardiovascular disease. we report here further observations on the presence and characteristics of this complex. some apparently healthy individuals who, on testing by standard methods, have normal levels of factor vii activity achieve such levels by means of a phospholipase c-sensitive modification of (some of) their factor vii ...	1986	3090680
survival and growth of bacillus cereus in bread.	bread doughs were artificially inoculated with spores of six bacillus cereus strains at different inoculum levels and counts of survivors in bread determined during storage at 27.5 degrees c. no b. cereus were isolated from the centre crumb of 400 g loaves when the dough contained less than 10(4) spores/g whereas with 800 g loaves survival occurred with doughs containing 5.0 x 10(3) spores/g. with all strains there was a period of at least 24 h before multiplication took place in the bread. the ...	1986	3091561
the primary structure of bacillus cereus neutral proteinase and comparison with thermolysin and bacillus subtilis neutral proteinase.	the complete amino-acid sequence of a neutral proteinase, produced by bacillus cereus, was determined by protein sequencing. the neutral proteinase consists of 317 amino-acid residues. the primary structure is 70% homologous to thermolysin, a thermostable neutral proteinase and 45% homologous to bacillus subtilis neutral proteinase. the zinc-binding site and the hydrophobic pocket of the active site are highly similar in all three proteinases. b. cereus neutral proteinase which is 20 degrees c l ...	1986	3092843
a hospital cafeteria-related food-borne outbreak due to bacillus cereus: unique features.	although bacillus cereus is a well-known cause of food-borne illness, hospital-related outbreaks of food-borne disease due to b. cereus have rarely been documented. we report a hospital employee cafeteria outbreak due to foods contaminated with b. cereus in which an outside caterer was employed to prepare the suspect meals. data were collected from 249 of 291 employees who had eaten either of the two meals. with a mean incubation period of 12.5 hours, 64% (160 of 249) of employees manifested ill ...	1986	3093397