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branhamella catarrhalis pneumonia. 19827112186
beta-lactamases of branhamella catarrhalis and their inhibition by clavulanic acid.three of four clinical isolates of branhamella catarrhalis from belgium produced a beta-lactamase identical to enzymes previously reported to occur in french and british isolates of this organism. one strain, however, produced a new type of beta-lactamase. both beta-lactamase types were readily inhibited by low concentrations of clavulanic acid.19826980625
staphylococcus aureus cowan i and branhamella catarrhalis as b lymphocyte mitogens. culture conditions for optimal dna synthesis and selective stimulation of human b lymphocytes.staphylococcus aureus cowan i and branhamella catarrhalis that selectively stimulate human b lymphocytes to increased dna synthesis in serum-free culture media were shown to do so also in 3 day cultures in medium containing human serum or albumin. after this time the transformed cells expressed little or no surface-bound immunoglobulin and did not form rosettes with sheep erythrocytes. when lymphocytes were cultured with bacterial mitogens in the presence of serum or albumin for more than 3 days ...19826980953
[respiratory tract infection with branhamella catarrhalis]. 19826984859
new method that uses binding of immunoglobulin a to group a streptococcal immunoglobulin a fc receptors for demonstration of microbial immunoglobulin a protease activity.a new method is described for the detection of bacterial immunoglobulin a (iga) protease which splits iga into fab and fc fragments. the method takes advantage of a recent finding that receptors for iga fragments occur commonly among type 4 group a streptococci. the bacterial preparation to be tested for protease activity was first incubated with radiolabeled purified iga1 myeloma protein, and the proportion of radioactivity bound to a standard suspension of the streptococci was then measured. s ...19817016917
branhamella catarrhalis as a human pathogen and a possible hospital infectant.branhamella catarrhalis, previously named neisseria catarrhalis and supposed to be a saprophytic inhabitant of the human upper respiratory tract, is a facultative pathogen. it was already proved by other authors to be able to cause serious and even lethal respiratory infections and purulent meningitis. our aim was only to call attention to its repeated and massive isolation of pure culture from nose and throat swabs of hospitalized children who mostly suffered from asthma bronchiale and spastic ...19817198350
branhamella catarrhalis pneumonia: report of two cases and review of the literature.branhamella (neisseria) catarrhalis is a saprophytic inhabitant of the human oropharynx with the capacity to cause infection, particularly in immunodeficient hosts. there have been 2 cases of branhamella catarrhalis pneumonia reported in the literature. two additional cases are described and the subject reviewed. an 80-yr-old woman with chronic lymphocytic leukemia presented with left lower lobe pneumonia. gram stain of transtracheal aspirate revealed intraleukocytic and extraleukocytic gram-neg ...19817235378
branhamella catarrhalis in respiratory secretions: clinical correlation in 16 cases.during a two-month period 24 of 1,060 (2.3%) samples of respiratory secretions submitted for culture yielded branhamella catarrhalis in quantities of more than ten colonies per primary plate. fifteen cultures were "pure" (ie, mixed only with normal throat flora). of the 16 patients with positive cultures, 14 showed preexisting disease, and 15 had respiratory symptoms. twelve of 13 patients studied showed more than 10 wbcs/lpf in the direct smear. it is concluded that b catarrhalis should be look ...19817280758
branhamella catarrhalis and other bacteria in the nasopharynx of children with longstanding cough.nasopharyngeal cultures from 180 children aged 1 to 9 were examined. all children were suffering from cough for at least 10 days. the findings were compared to those from 67 non-coughing children. bordetella pertussis was isolated from 12.2% of the children in the study group but from none of the control children. branhamella catarrhalis was isolated from 66.1% of the children in the study group and from 28.3% in the control group (p less than 0.01). br. catarrhalis was more common in pure cultu ...19817313564
preliminary serologic evidence for a pathogenic role of branhamella catarrhalis.branhamella catarrhalis has been suspected, on the basis of bacteriologic culture results, to have a pathogenic role in 6%-9% of cases of acute otitis media as well as in other upper respiratory tract infections. serologic evidence of this role was obtained with use of an enzyme immunoassay to detect antibodies to b. catarrhalis. the presence of both igg and iga antibodies to branhamella in the serum and/or middle ear fluid (mef) of children with acute otitis media correlated with the isolation ...19817328328
viability of six species of normal oropharyngeal bacteria after exposure to cigarette smoke in vitro.bacteria in culture medium at 37 degrees c were subjected to eight puffs of smoke from one cigarette, and viable cell counts were done at intervals ranging from 0 min to 3 h after exposure to smoke. the cigarette contained 23.0 mg of tar and 1.4 mg of nicotine. each 25.0 ml puff of smoke from a mechanical smoking apparatus was passed over a 1.0 ml suspension of bacteria dispersed over a 25 cm2 surface in a sterile flask. filtered air was in contact with the suspension between puffs, and during t ...19817339446
ophthalmia neonatorum caused by penicillin-resistant branhamella catarrhalis. 19816949055
otitis media caused by beta-lactamase-producing branhamella (neisseria) catarrhalis.a beta-lactamase-producing branhamella catarrhalis was isolated in pure culture from the right middle ear aspiration of an otitis media in a 3-month-old girl. the patient responded well to cefamandole treatment.19816970205
acute maxillary sinusitis in children.we sought to correlate the clinical, radiographic, and bacteriologic findings in maxillary sinusitis in 30 children who had both upper-respiratory-tract symptoms and abnormal maxillary radiographs. cough, nasal discharge, and fetid breath were the most common signs, but fever was present inconsistently. facial pain or swelling and headache were prominent symptoms in older children. bacterial colony counts of greater than or equal to 10(4) colony-forming units per milliliter were found in 34 of 4 ...19816970333
branhamella catarrhalis: antibiotic susceptibility and beta-lactamase production. 19816971288
formalin-treated bacteria as selective b cell mitogens: results in primary and acquired immunodeficiencies.the mitogenic activity of the formalin-treated bacterial strains branhamella catarrhalis, haemophilus influenzae and the cowan i strain of staphylococcus aureus was assessed in peripheral blood lymphocytes (pbl) from patients with primary immunodeficiencies, acute lymphocytic leukaemia (all), chronic lymphocytic leukemia (cll) and in umbilical cord blood lymphocytes. the bacteria selectively stimulated b cells, as demonstrated by the finding of a normal de novo dna synthesis in children with a t ...19816976247
formalin-treated bacteria as selective b cell mitogens in the study of lymphocytes from patients with hypogammaglobulinaemia.staphylococcus aureus strain cowan i, haemophilus influenzae, and branhamella catarrhalis that stimulate human b lymphocytes, but not t lymphocytes, were used to study the b lymphocyte function in patients with hypogammaglobulinaemia. lymphocytes from two patients with no circulating b cells were not stimulated to increased dna synthesis by these bacterial mitogens. the lymphocytes of six patients with hypogammaglobulinaemia and normal numbers of circulating b cells responded to the bacterial mi ...19806971196
branhamella (neisseria) catarrhalis: criteria for laboratory identification.eleven clinically significant isolates of branhamella catarrhalis grew well on modified thayer-martin medium and produced beta-lactamase, but did not grow on nutrient agar at 22 degrees c. minimum inhibitory concentrations of vancomycin, colistin, and trimethoprim were found to be higher than the concentrations of these antibiotics in modified thayer-martin medium. the criteria necessary for laboratory identification of b. catarrhalis are discussed.19806965679
disparate cultures of middle ear fluids. results from children with bilateral otitis media.cultures of middle ear fluids (mefs) are needed to determine both efficacy of antibiotics and vaccines, and microbiologic outcome of otitis media (om). we reviewed data on 221 children, aged 2 months to 12 years; 122 had acute otitis media (aom), 99 had asymptomatic mef. we included only streptococcus pneumoniae, haemophilus influenzae, branhamella catarrhalis, and staphylococcus aureus as pathogens. of children with aom, mef was sterile or contained nonpathogens in both ears in 51, and one or m ...19806968508
[penicillin-resistant branhamella catarrhalis. frequency and appropriate therapy]. 19806907614
[immunologic characteristics of protein antigens of the neiseriaceae family. iii. study of delayed hypersensitivity cross reactions between different species of neisseria].the immunological interrelations between the protein complexes of 34 strains of 12 species belonging to the family neisseriaceae were studied with the use of delayed hypersensitivity reactions in specifically sensitized guinea pigs. no statistically significant differences between the nonpathogenic species of the family neisseriaceae and the strains of n. haemolysans, n. cuniculi, n. lactamicus, n. ovis, n. elongata, n. cinerea were revealed. the preparations of branhamella catarrhalis and n. ca ...19807415672
branhamella keratitis.three cases of suppurative keratitis caused by branhamella catarrhalis are described. each presented as a localised stromal infiltrate in a previously scarred cornea. the condition responded to penicillin g and to gentamicin treatment.19807448142
high isolation rate of branhamella catarrhalis from the nasopharynx in adults with acute laryngitis.branhamella catarrhalis was isolated from the nasopharynx in 55% of 40 noncompromised adult patients suffering from acute laryngitis. diplococcus pneumoniae and haemophilus influenzae were found in another 5 and 8%, respectively; one patient had group a streptococci in the throat specimen. in 90% of the patients the laryngitis complaints were preceded by symptoms of an acute respiratory tract infection. two of the patients with b. catarrhalis showed a significant titre conversion against influen ...19807006061
antibiotic susceptibility of beta-lactamase-producing strains of branhamella (neisseria) catarrhalis.all 11 clinically significant isolates of branhamella catarrhalis examined in this study were found to produce beta-lactamase. the enzyme was apparently not plasmid associated since extrachromosomal deoxyribonucleic acid was not detected in any of the strains. the beta-lactamase activity of all strains was significantly depressed by the beta-lactamase inhibitors clavulanic acid and cp 45899. based on comparisons of relative susceptibility to various beta-lactam antibiotics, it was inferred that ...19806766292
penicillinase-producing neisseria gonorrhoeae in the netherlands: epidemiology and genetic and molecular characterization of their plasmids.penicillinase-producing neisseria gonorrhoeae strains were isolated in the netherlands with increasing frequency during the period of 1976 to 1979. about 3% of the gonococci isolated in the first half of 1979 produced penicillinase. in contrast to the period of 1976 to 1977, most penicillinase-producing n. gonorrhoeae infections during the period of 1978 to 1979 were contracted in the netherlands. the results of genetic and molecular studies on 80 penicillinase-producing n. gonorrhoeae strains w ...19806778387
a protein antigen characteristic of branhamella catarrhalis. serological identification of the genus.precipitation patterns of sonicated, acid-extracted and other extracts from branhamella catarrhalis were examined by double diffusion-in-gel technique, using antiserum to b. catarrhalis. acid extract gave rise to 4 distinct precipitates. one of these lines was further studied. the bacterial component responsible for this line was trypsin-sensitive, indicating that it was a protein. it was anodally localized by crossed immunoelectrophoresis. by absorption of antiserum with whole bacteria, the pre ...19806781219
upper respiratory tract spread of group b streptococci type i b in a kindergarten.in a kindergarten with 42 children and 17 female staff members, an epidemic of group b streptococcal carriage in the upper respiratory tract occurred. in the middle of february 1978, 6 children and 5 adults carried type i b streptococci in the throat while only 2 of these 11 were carriers 2 weeks later. only one other streptococcus, belonging to type ii, was found in the throat specimens. five strains other than type i b were found in the urogenital tract of the staff. three type i b throat carr ...197937592
neisseriaceae, a group of bacteria with dihydrofolate reductases, moderately susceptible to trimethoprim.dihydrofolate reductases of five species of the family neisseriaceae were compared by means of inhibition profiles, using several structurally different inhibitors, including trimethoprim (tmp) and pyrimethamine. all enzymes were seen to be highly susceptible to the folate analog aminopterin, but exhibited moderate susceptibility to all other inhibitors tested. approximately 200-fold higher concentrations of tmp were needed to inhibit neisserial reductases as compared to the e. coli enzyme. besi ...197944939
lectins in diagnostic microbiology: use of wheat germ agglutinin for laboratory identification of neisseria gonorrhoeae.a lectin slide agglutination test has been developed for the confirmatory identification of neisseria gonorrhoeae. with wheat germ lectin as an agglutinin, 164 of 165 clinical isolates of n. gonorrhoeae gave a 3 to 4+ reaction within 6 to 8 min. four gonococcal isolates, even though negative by the fluoresecent-antibody method, gave strong positive reactions with the wheat germ lectin. among 23 isolates of neisseria meningitidis tested, which included representatives of sero-groups a, b, c,d, x, ...197994598
dihydrofolate reductase from neisseria sp.members of the genus neisseria are relatively nonsusceptible to trimethoprim, an inhibitor of dihydrofolate reductase. for example, the minimal inhibitory concentration (mic) of trimethoprim for n. gonorrhoeae ranges from 2 to 70 mug/ml, whereas the mic for escherichia coli is 0.2 mug/ml or less. in an effort to understand this difference, dihydrofolate reductase was partially purified from five neisseria species and compared with the enzyme from e. coli. n. gonorrhoeae dihydrofolate reductase w ...1979111611
the role of natural igg and complement in the phagocytosis of type 4 neisseria gonorrhoeae by human polymorphonuclear leukocytes.the role of human serum components in the phagocytosis of logarithmic-phase type 4 neisseria gonorrhoeae by human polymorphononuclear leukocytes was investigated. the requirement of fresh normal human serum (fhs) for optimal phagocytosis and the fixation of human immunoglobulin (igg) and complenet (c3) to the gonococcal cell surface suggested that both serum factors participate in the phagocytosis of these organisms. the percentage of neutrophils containing ingested organisms was directly propor ...1979118998
evaluation of spiramycin as a therapeutic agent for elimination of nasopharyngeal pathogens. possible use of spiramycin for middle ear infections and for gonococcal and meningococcal nasopharyngeal carriage.varying doses of spiramycin were administered orally to healthy volunteers, and concentrations in serum and saliva were determined. the absorption of the drug was not significantly influenced by concomitant food intake. saliva peak concentrations were 1.3--4.8 times higher than peak concentrations in serum. the elimination half life was 2--3 h in serum, and 4--8 h in saliva. accumulation of the drug was seen in saliva but not in serum. the possible effect of spiramycin in eliminating bacteria fr ...197897775
bronchopulmonary infection due to beta-lactamase-producing branhamella catarrhalis treated with amoxycillin/clavulanic-acid. 197879045
the lipopolysaccharide (r type) as a common antigen of neisseria gonorrhoeae. ii. use of hen antiserum to gonococcal lipopolysaccharide in a rapid slide test for the identification of n. gonorrhoeae from primary isolates and secondary cultures.an antiserum has been prepared in hens to r-type gonococcal lipopolysaccharide (lps) and used in a simple slide-agglutination test for the identification of neisseria gonorrhoeae. anti-lps serum agglutinated gonococcal cells representative of the four colony types of n. gonorrhoeae. absorption of the antiserum with lps removed the agglutinating activity. secondary cultures (1120) were tested without observation of the colony type and all were agglutinated. no agglutination occurred with strains ...1978417781
immunological relationships of some non-pathogenic neisseria.a collection of nine stock strains of non-pathogenic neisseria species and one strain of branhamella catarrhalis has been examined serologically by a simple immunodiffusion technique utilising cultures growing on solid medium.1978417785
bronchopulmonary infection due to branhamella catarrhalis: 11 cases assessed by transtracheal puncture.transtracheal punctures were performed in 193 miners of anthracosilicotic coal who presented with an acute exacerbation of chronic bronchitis. the transtracheal aspirates were bacteriologically positive in 104 patients. branhamella catarrhalis was isolated in 15 patients; 10 of these responded to chemotherapy, as shown by the resolution of all symptoms and the disappearance of the organism on a repeat transtracheal puncture performed 48 hours after the end of treatment. one ampicillin-treated pa ...1978620304
binding of hla antigen-containing liposomes to bacteria.highly purified, detergent-solubilized hla-a and -b antigens and hla-d antigens were separately incorporated into liposomes. detergent-solubilized transplantation antigens, but not papain-solubilized antigens lacking the membrane-integrated portions of the molecules, were bound to the liposomes. a considerable portion of the liposome-bound antigens displayed accessible antigenic sites, suggesting that they were oriented in the right-side-out direction. liposomes containing the hla-a and -b antig ...1978282637
novel beta-lactamase from branhamella catarrhalis.a strain of branhamella catarrhalis isolated from sputum was shown to be resistant to penicillin and ampicillin. this strain produced a beta-lactamase which has been judged to be a novel enzyme, on the basis of substrat profil, isoelectric point and location of genetic determinant.1978311606
in vitro sensitivity to penicillin v and beta-lactamase production of branhamella catarrhalis. 1977408320
neisseria meningitidis in the anal canal of homosexual men.cultures were obtained from the oropharynx, urethra, and anal canal of 157 homosexual men. neisseria gonorrhoeae was identified in 30 men (19.1%), neisseria meningitidis in 32 men (20.4%), and neisseria lactamicus and branhamella catarrhalis in one man each. n. gonorrhoeae was isolated from the anal canal of three men (1.9%), and n. meninititidis was identified in the anal canal of seven men (4.5%). these findings suggest that confirmatory tests are indicated for homosexual males with rectal iso ...1977411836
fatal neisseria (branhamella) catarrhalis pneumonia in an immunodeficient host.a 64-year-old man with far-advanced iga multiple myeloma presented with fulminant and rapidly fatal bronchopneumonia. gram stains of expectorated sputum and of transtracheal aspirate revealed gram-negative intracellular and extracellular diplococci. cultures of these specimens yielded neisseria catarrhalis. subsequent histologic examination of the lung confirmed the presence of numerous gram-negative diplococci in the alveolar spaces. the pathogenic potential of this nasopharyngeal commensal is ...1976788567
lipids of branhamella catarrhalis and neisseria gonorrhoeae.three strains of branhamella catarrhalis and three strains of neisseria gonorrhoeae were analyzed with regard to their phospholipid and neutral lipid composition. b. catarrhalis (atcc 23246) contained 5.12 +/- 0.34% lipid, determined gravimetrically, compared to 8.56 +/- 0.15% and 9.73 +/- 0.06% for two strains of n. gonorrhoeae. cardiolipin, phosphatidylglycerol, and phosphatidyl-ethanolamine were identified in extracts of both species. in addition, b. catarrhalis contained small amounts of pho ...1976819418
studies on branhamella catarrhalis (neisseria catarrhalis) with special reference to maxillary sinusitis.paired sera from 97 patients with acute maxillary sinusitis were examined regarding antibodies to branhamella catarrhalis. precipitating antibodies were demonstrated in almost all sera both from patients and from healthy blood donors. complement-fixing (cf) antibodies to b. catarrhalis were present in sera from 25 of the 97 patients and in one of 20 healthy blood donors. the titres were low and the titre changes when present were of a small magnitude. cf antibodies were most commonly demonstrate ...1976823639
comparison of the cell envelope proteins of micrococcus cryophilus with those of neisseria dnd branhamella species.in an attempt to elucidate the relation between micrococcus cryophilus, neisseria caviae, neisseria ovis, and branhamella catarrhalis, fractions derived from outer membranes of a strain of each organism were examined for protein composition by sds - polyacrylamide gel electrophoresis. micrococcus cryophilus outer membrane protein showed extensive similarities to that of n. ovis and contained a heat-modifiable protein which behaved almost identically with the corresponding bands previously shown ...19761260534
studies on the cellular and free lipopolysaccharides from branhamella catarrhalis.cellular and free lipopolysaccharides obtained from neisseria catarrhalis and branhamella catarrhalis were found to be essentially identical. both cellular and free lipopolysaccharides contained core-oligosaccharides of the following composition: d-glucose (4 mol), d-galactose (1 mol), 2-amino-2-deoxy-d-glucose (1 mol), and 3-deoxy-d-manno-octulosonic acid. aldoheptose and phosphate components were below levels of detection. several physical methods indicated that all core-oligosaccharide prepar ...19761260539
metabolic requirements for entry of dna in branhamella catarrhalis.although requirements for transformation in branhamella catarrhalis are quite complex, dna synthesis does not appear to be one of these needs, as indicated by the inability of nalidixic acid to interefere with transformation. exogenous sources of energy, such as camp and cgmp also failed to enhance frequency, suggesting cells may actively engage in energy production to achieve uptake of dna, or lack a transport mechanism for these compounds.1976183979
enzyme electrophoretograms in the analysis of taxon relatedness of micrococcus cryophilus, branhamella catarrhalis and atypical neisserias.extracts were prepared from micrococcus cryophilus, several strains of branhamella catarrhalis and neisseria spp. esterases, nadp-dependent isocitrate dehydrogenase and malate dehydrogenase activities were assayed after electrophoresis of extracts of polyacrylamide gels. except for neisseria perflava and n. sicca which resolved activity bands for the acetate-esterase only, the remaining bacteria exhibited species-specific esterase patterns also for the propionate and butyrate substrates. the mul ...1975234507
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