Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
boutonneuse fever transmitted by conjunctival inoculation.we report three cases (two adult males and a 12-year-old child) of boutonneuse fever produced by inoculation of the conjunctival mucosa (probably through accidental entry of r. conorii fluid into the eyes through rubbing or splashing). all three patients developed similar symptoms of disease, including high fever, headache, maculopapular exanthem, and conjunctivitis, and none had tache noire. specific immunofluorescent antibodies appeared in these patients' serum mostly after the 2nd week; agglu ...19883236764
[are cardiac glycosides advisable in the treatment of food poisoning].rheopolycardiological investigation of 76 patients with food toxinfections (fti) showed that phasic changes of systolic indices in these diseases developed as a result of extracardiac hemodynamic disorders and were of adaptive nature. strophanthin administration to 20 similar patients at the height of disease brought about no statistically significant changes in systolic indices. it was concluded that the use of cardiac glycosides was inappropriate for the treatment of fti.19883238582
report of a case resembling the proteus syndrome with a chromosome abnormality. 19883239588
in vitro susceptibility of bacteria to a ticarcillin-clavulanic acid combination.in vitro testing of bacterial susceptibility to a combination of ticarcillin and clavulanic acid was done, using 406 aerobic gram-positive and gram-negative isolates (considered to be pathogens) cultured from equine and small animal specimens. a microdilution broth technique of susceptibility testing was performed, using trays with wells containing a range of doubling concentrations of dehydrated ticarcillin (range, 0.50 to 128 micrograms/ml) with fixed concentration of clavulanic acid (4 microg ...19883239838
activity of cefixime (fk 027) for resistant gram-negative bacilli.cefixime is an orally absorbable cephalosporin with an extended spectrum of in vitro activity for gram-negative bacteria especially members of the enterobacteriaceae. gram-negative rod isolates collected over a three month period were tested against cefixime. greater than 99% of escherichia coli isolates was susceptible to cefixime including those resistant to ampicillin. similarly isolates of klebsiella, proteus and providencia were also exquisitely susceptible to cefixime as were 91% of entero ...19883243091
[rifampicin in the treatment of infections of non-tuberculous etiology].clinical efficacy of rifampicin, a semisynthetic broad spectrum antibiotic was estimated in 247 patients with purulent inflammations. it was shown advisable to use rifampicin intravenously in treatment of severe bronchopulmonary pathology, disorders of the bile excretion system, osteomyelitis, severe wound infections and in prophylaxis of postoperative purulent complications in cardiovascular surgery and other cases. high rifampicin sensitivity of staphylococci and streptococci belonging to vari ...19883245710
[clinical studies on sultamicillin fine granule in pediatric field].pharmacokinetics, safety and effects on bacterial infection of sultamicillin (sbtpc) fine granule were evaluated in 17 children. the results obtained are summarized as follows. 1. pharmacokinetics in 3 children receiving a single dose of 10 mg per kg body weight were evaluated. the half-life of ampicillin (abpc) was 1.38 +/- 0.14 hours and that of sulbactam was 0.93 +/- 0.26 hour. 2. fourteen cases, including 7 tonsillitis, 2 pharyngitis, 2 bronchitis, and 1 each of cystitis, scarlet fever and c ...19883249370
[the in vitro activity of ofloxacin, pefloxacin and ciprofloxacin against various gram negative bacteria isolated from urinary tract infections].in this study the in vitro activity of ofloxacin, pefloxacin and ciprofloxacin against various gram negative bacteria isolated from urinary tract infections was examined. of 80 isolates (24 e. coli, 10 enterobacter, 10 klebsiella, 23 proteus, 10 pseudomonas, 2 morganella and 1 serratia) 49 were from inpatients, and 31 were from outpatients. ciprofloxacin was the most potent compound and no resistant strain was found against ciprofloxacin, while 1 enterobacter strain and 1 pseudomonas strain were ...19883252111
[causative agents of urinary tract infections and sensitivity to antibiotics].our study was done on the outpatients and inpatients of ankara numune hospital who were diagnosed as having urinary tract infections. when 301 cultures with positive results were evaluated. gram-negative bacteria were found most frequently (e. coli 45.2%, klebsiella 23.2%, enterobacter 16.6%, proteus 11.5%, s. aureus 2.2%, pseudomonas 1.3%). by using the disc diffusion method, the effectiveness of antibiotic discs of amikacin, tobramycin, netilmycin, cephoperazone, cephotoxime, cephtizoxime, cep ...19883252113
[a new rapid antibiotic susceptibility test for enteric bacteria using a color change method].the rapid antibiotic susceptibility tests that have been developed so far cannot be used in daily work, because of their many difficulties and disadvantages. we have developed a new antibiotic susceptibility test for enteric bacteria which gives the result in 4 hours, easy to perform and inexpensive. this method depends upon the mechanism which detects the acid formed by the bacteria, by the change of the color of the ph indicator in the medium. the susceptibility of 110 different isolates of en ...19883252116
[antibiotic-resistant microorganisms isolated from the urine of patients with suspected urinary tract infections].in our study 5331 urine specimen have been examined. in 22 of the total 2 types of bacteria have been isolated and in the cultures of 1167 patients colony counts over than 100.000/ml. have been determined. these are 599 escherichia coli, 161 hemolytic escherichia coli, 104 klebsiella, 93 proteus, 81 pseudomonas, 65 staphylococcus epidermidis, 44 staphylococcus aureus, 24 enterobacter, 18 enterococcus. 602 of the infected patients were women and 565 were men. according to antibiogram results micr ...19883252120
[climate therapy and change in cellular immunity].the cell-mediated immunity was investigated by delayed hypersensitivity reactions on recall antigens (tetanus, diphtheria, streptococci, tuberkulin, candida, trichophyton, proteus/multitest-merieux) in 56 patients with atopic dermatitis (34 females, 22 males) under mountain-climatic therapy (davos, 5118 ft). the testscore of 7.9 mm (females 8.2/males 7.5) was lowered in comparison to the normals within the literature (females 15.4/males 21.0). anergy was found in 8.8% in females (normal 4%) and ...19883252623
an evaluation of allozyme amino acid substitutions for the study of molecular relationships in providencia strains.through analysis of molecular relationships in terms of amino acid substitutions, intra- and interspecies differentiations in providencia alcalifaciens, p. stuartii and p. rustigianii were evaluated among the electrophoretic variants of three enzymes, l-malate dehydrogenase, acid phosphatase and esterase-beta a, chosen for their distinct pattern of polymorphism. for each enzyme, molecular relatedness among variants defined by two-dimensional electrophoretic profiles was examined through protein ...19883252906
occurrence, transfer and mobilization in epilithic strains of acinetobacter of mercury-resistance plasmids capable of transformation.a 7.8 kb plasmid (pqm17) encoding mercury resistance was isolated from two epilithic strains of acinetobacter calcoaceticus. the plasmid had a broad host range when mobilized by rp1, transferring into pseudomonas aeruginosa, p. putida, p. fluorescens, escherichia coli, proteus vulgaris and chromobacterium sp. with frequencies ranging from 5.3 x 10(-9) to 4.6 x 10(-4) per recipient. the plasmid could be transferred into a. calcoaceticus bd413 using intact cells of donor and recipient bacteria (i. ...19883254940
bmy-28100, a new oral cephalosporin: antimicrobial activity against nearly 7,000 recent clinical isolates, comparative potency with other oral agents, and activity against beta-lactamase producing isolates.the antimicrobial activity of bmy-28100 was tested against approximately 7,000 bacterial pathogens in a multicenter, multiphased collaborative investigation. the bmy-28100 spectrum and antimicrobial potency was most similar to that of cefaclor and superior to that of cephalexin among currently available cephalosporins. species that had greater than or equal to 90% of clinical strains inhibited by bmy-28100 (less than or equal to 8.0 micrograms/ml) were: citrobacter diversus, escherichia coli, kl ...19883259489
antibacterial activity in human urine of fosfomycin trometamol in an in vitro model of the urinary bladder.the urinary concentrations of fosfomycin trometamol, norfloxacin, pipemidic acid and cotrimoxazole were studied at various times after oral administration of drugs in healthy volunteers. using the same urine, the bactericidal activity of four antimicrobial agents against escherichia coli, proteus mirabilis and klebsiella pneumoniae in an in vitro model simulating the treatment of bacterial cystitis was also evaluated. the results obtained show that very high concentrations of the drugs were achi ...19883259909
treatment of experimental cystitis in the rat with a single dose of fosfomycin trometamol.the therapeutic effectiveness of a single oral dose (60 and 200 mg/kg body weight) of fosfomycin trometamol (ft), norfloxacin, trimethoprim sulfamethoxazole (bactrim) and pipemidic acid against experimental cystitis in the rat were compared. infections were produced with clinical isolates of klebsiella pneumoniae, proteus mirabilis and escherichia coli in a total of 135 sprague-dawley albino rats. oral treatment with all four drugs consistently lowered the numbers of cfu in bladder tissue, espec ...19883259910
in vitro activity of an oral iminomethoxy aminothiazolyl cephalosporin, r-3746.the in vitro activity of r-3746, an iminomethoxy aminothiazolyl cephalosporin with a ch2och3 moiety at position 3, was compared with those of other antibiotics. r-3746 inhibited the majority of hemolytic streptococci (groups a, b, c, f, and g) and streptococcus pneumoniae at less than 0.06 micrograms/ml, which was comparable to the activity of amoxicillin, 2- to 8-fold more active than cefixime, and 16- to 64-fold more active than cefaclor and cephalexin. ninety percent of beta-lactamase-produci ...19883260766
comparable evaluation of orally active beta-lactam compounds in ampicillin-resistant gram-positive and gram-negative rods: role of beta-lactamases on resistance.the antibacterial activity of the recently developed cephems cefixime and cefetamet-pivoxyl was evaluated in 408 gram-positive and gram-negative rods, all isolated recently from clinical specimens, and compared to that of other orally active agents such as ampicillin, amoxycillin + clavulanic acid, cefaclor, cefuroxime-axetil and to ceftriaxone. with regard to ampicillin-resistant enterobacteriaceae ceftriaxone proved to be the most active agent, followed by cefixime and cefetamet, whereas cefur ...19883262045
in-vitro activity and beta-lactamase stability of ly163892.ly163892 is a new orally absorbed carbacephem. it inhibited streptococcus pyogenes and str. pneumoniae at less than or equal to 1 mg/l, but was less active against group b streptococci and groups c, f, g and bovis streptococci with mics of 1 to 2 mg/l for most but as high as 8 mg/l for some isolates. mic90 of methicillin-susceptible staphylococcus aureus was 8 mg/l, but greater than 128 mg/l for methicillin-resistant staphylococci. ly163892 had activity similar to cefaclor and cephalexin with mi ...19883263352
activity of temocillin and other penicillins against beta-lactamase-inducible and -stably derepressed enterobacteria.mics of temocillin, carbenicillin, ticarcillin, mezlocillin, piperacillin and ampicillin were determined for mutant series of enterobacter cloacae, citrobacter freundii, proteus vulgaris, morganella morganii and serratia marcescens with inducible, stably derepressed or basal expression of chromosomal class i beta-lactamases. ampicillin was inactive (mic greater than 256 mg/l) both against beta-lactamase-inducible organisms (except c. freundii) and their stably derepressed mutants, whereas basal ...19883263354
simple assay of beta-lactamase with agar medium containing a chromogenic cephalosporin, pyridinium-2-azo-p-dimethylaniline chromophore (padac).a new beta-lactamase assay method with agar plates containing pyridinium-2-azo-p-dimethylaniline chromophore (padac) (50 microm), a beta-lactamase-labile, chromogenic cephalosporin, was examined. on the padac plates inoculated with beta-lactamase-producing gram-negative bacteria (10(4) cfu per spot) and incubated at 37 degrees c, a yellow zone showing hydrolysis of padac by beta-lactamase was formed around the colony. the zone diameter increased with incubation time. examination with enterobacte ...19883263833
in vitro activity of the combination trimethoprim plus sulfamethoxazole compared with that of other chemotherapeutic agents.for many years now, the combination trimethoprim plus sulfamethoxazole (active ingredients of bactrim) has proved to be an effective chemotherapeutic agent with a broad spectrum of antibacterial activity against both gram-positive and gram-negative organisms, including beta-hemolytic streptococci, staphylococci, pneumococci, haemophilus influenza, escherichia coli, proteus mirabilis, proteus vulgaris, klebsiella, enterobacter and citrobacter. in this comparative study, the antibacterial activity ...19883264166
in vitro antibacterial activity of fk482, a new orally active cephalosporin.fk482 is a new orally active cephem antibiotic which offers some advantages over the commercially available oral beta-lactam antibiotics. it displayed a broad spectrum of activity in vitro against stock strains of gram-positive and gram-negative aerobes and anaerobes. fk482 was more active in vitro than cefixime (cfix), cefaclor (ccl) or cephalexin (cex) against clinical isolates of gram-positive organisms such as methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococci incl ...19883264828
[urine bacterial flora and chemoantibiotic resistance: observations on ambulatory patients and hospitalized patients at the local health district no. 11 of fabriano (ancona)].this paper reports the results of a study carried out on 4614 samples of urine from inpatients and outpatients according to the sex. the positive monomicrobic urine specimens were examined and isolated strains were tested against various antimicrobial drugs. our findings showed that 43.1% of urine specimens examined from inpatients and 38.4% from outpatients proved to be positive. among the bacterial species more frequently isolated, significant differences among enterobacter spp. in males and c ...19883268917
[aerobic and anaerobic bacterial flora of crural ulcers].in a group of 600 patients treated in the metropolitan dermatological hospital in warsaw bacteriological examination were carried out of swabs from the untreated crural ulcers. in 95% of these cultures growth of pathological aerobic organisms was obtained. coagulase-positive staphylococci (st. aureus) and gram-negative bacteria (pseudomonas aeruginosa, proteus vulgaris, enterobacter sp and e. coli) prevailed. in 27% of cases the cultured strains were resistant to the generally available antibiot ...19883268941
killing of proteus mirabilis by polymorphonuclear leukocyte granule proteins: evidence for species specificity by antimicrobial proteins.low-molecular-weight (mr, ca. 3,800) polypeptides containing human defensins hnp-1 and hnp-2 (t. ganz, m. s. selsted, d. szlarek, s. l. harwig, k. daher, d. f. bainton, and r. i. lehrer, j. clin. invest. 76:1427-1434, 1985) prepared in our laboratory from acid extracts of human polymorphonuclear granulocyte granules and purified human defensins were found to exert potent bactericidal action against proteus mirabilis. the antimicrobial action of the extracts of human polymorphonuclear leukocytes ...19883275586
production and properties of haemolysins from clinical isolates of the proteeae.a collection of 198 clinical isolates of strains belonging to the tribe proteeae was examined for haemolytic activity on blood agar and in brain heart infusion broth. the strains were of diverse bacteriocin and o-antigenic types and from a wide variety of sources. they included representatives of all species of morganella, proteus and providencia. approximately half of the m. morgani strains were haemolytic on blood agar. this activity was not associated with any particular bacteriocin type. the ...19883275780
a survey of iga protease production among clinical isolates of proteeae.a collection of 100 strains of proteeae, in which all species within the tribe were represented, was examined for iga protease production. the strains were isolated from various clinical specimens from sick and healthy persons in several countries. iga protease-producing strains were not found amongst species of providencia and morganella but were common in proteus spp. all the strains of p. mirabilis and p. penneri and many of the strains of p. vulgaris examined produced an edta-sensitive prote ...19883275781
rapid antibiotic susceptibility tests on enterobacteriaceae by atp bioluminescence.the susceptibility of 76 clinical isolates of enterobacteriaceae to ampicillin, piperacillin and gentamicin was assessed by atp bioluminescence in a 4-h test. for most organisms tested (escherichia, klebsiella, enterobacter and serratia), there was good correlation with traditional mic values estimated on 18-h cultures. however strains of proteus mirabilis showed false resistance to the beta-lactam agents with the atp method; and concordance was achieved only after manipulation of the growth con ...19883276899
cloning of the recb, recc, and recd genes from proteus mirabilis in escherichia coli: in vivo formation of active hybrid enzymes.we cloned chromosomal dna fragments from proteus mirabilis which complement recbcd deletion mutants of escherichia coli by restoring (i) recombination proficiency in conjugation, (ii) normal resistance to uv irradiation, and (iii) atp-dependent exonuclease activity for duplex dna. the data indicate that the order of the genes thya, recc, recb, recd, and arga is similar in both p. mirabilis and e. coli. hybrid enzymes formed in vivo were active in repair and recombination.19883277957
commercial identification systems often fail to identify providencia stuartii.we tested 145 clinical isolates in an attempt to evaluate some of the most widely used commercial identification systems in europe in terms of their ability to identify providencia strains. two manual miniaturized systems (api 20e and enterotube ii) and three mechanized-automated systems (cobas-bact, sceptor system, and titertek-enterobac-rapid automated system) were evaluated. providencia alcalifaciens and providencia rettgeri strains were correctly identified by all systems in all cases, and i ...19883277997
tigemonam, an oral monobactam.tigemonam is an orally administered monobactam. at less than or equal to 1 microgram/ml it inhibited the majority of strains of escherichia coli, klebsiella spp., enterobacter aerogenes, citrobacter diversus, proteus spp., providencia spp., aeromonas hydrophila, salmonella spp., shigella spp., serratia marcescens, and yersinia enterocolitica. at less than or equal to 0.25 microgram/ml it inhibited haemophilus spp., neisseria spp., and branhamella catarrhalis. it did not inhibit pseudomonas spp. ...19883279906
structural studies on the core and lipid a region of a 4-amino-l-arabinose-lacking rc-type mutant of proteus mirabilis.the structure of the 4-amino-l-arabinose-lacking lipopolysaccharide of the proteus mirabilis rc-type mutant r4, derived from wild-type o28, was elucidated. the lipopolysaccharide core structure has previously been partially characterized. the linkage between heptose and deoxyoctulosonic acid(docla) is now reported, as well as the structure of the lipid a moiety of this mutant strain. besides the tentative identification of an alpha-linked glucosamine disaccharide in the lipid a backbone accompan ...19883280311
thrombocytosis and a renal abscess. 19883281051
proteus syndrome: ocular complications.proteus syndrome is a recently recognized hamartoneoplastic malformation syndrome of uncertain etiology and variable expression, whose cardinal manifestations are pigmented nevi, hemihypertrophy, macrodactly, lipomata, and cerebroid-gyriform configuration of the skin on the soles of the feet. the characteristic features may be present at birth but become more apparent with time. in the past this syndrome has been confused with other overgrowth disorders such as neurofibromatosis, klippel-trenaun ...19883282059
comparison of translocation rates of various indigenous bacteria from the gastrointestinal tract to the mesenteric lymph node.bacterial translocation is defined as the passage of indigenous bacteria from the gastrointestinal (gi) tract through the lamina propria to the mesenteric lymph nodes (mln) and other organs. we compared the relative abilities of various aerobic, facultatively anaerobic, and obligately anaerobic bacteria to translocate from the gi tract to the mln in gnotobiotic mice colonized with single strains of bacteria. indigenous gram-negative enteric bacilli translocated in large numbers to the mln, where ...19883283254
[bacterial spondylitis]. 19883283510
[experimental comparison of maxon and chromic catgut in suturing of the urinary bladder].the new monofile absorbable suture material maxon was compared with chromic catgut in bladder suturing in rabbits. comparisons were made after 1, 2, 4, 8 and 12 weeks. the rate of stone formation was mainly determined by the suture technique used and not so much by the suture material. this was demonstrated by the low number of stones formed after using extramucosal suturing technique. once a suture, be it maxon or chromic catgut, comes in contact with urine, concrements may form and the new mon ...19883284147
a positive response to any of seven intradermal antigens predicts favorable outcome in patients hospitalized with community-acquired pneumonia.we used the commercially available multitest (r) cmi to assess the response of 100 adults hospitalized with community-acquired pneumonia to the following seven antigens: tetanus toxoid, diphtheria toxoid, streptococcus, proteus, tuberculin, candida, and trichophyton. thirty-one of the patients responded to one or more of these antigens and survived their acute illness. of the multitest (r) cmi negative patients, 49 lived and 20 died. a comparison of the three groups revealed that the 31 patients ...19883284671
is pelvic inflammatory disease an indication for treatment with ciprofloxacin?this pilot study was carried out on 23 gynaecological patients suffering from salpingitis, salpingitis and pelviperitonitis, douglas' abscess, and vaginal stump abscess. 21 courses were evaluable for clinical efficacy. the diagnoses had been established mainly by pelviscopy and by clinical gynaecological examinations. the dosage was 2 x 500 mg ciprofloxacin orally every 12 h for 7.6 (5-11) days. cervical smears collected before therapy revealed the most common pathogens to be escherichia coli an ...19883286515
conservation of an atp-binding domain among reca proteins from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli k-12 and b/r.the purified reca proteins encoded by the cloned genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r were compared with the reca protein from e. coli k-12. each of the proteins hydrolyzed atp in the presence of single-stranded dna, and each was covalently modified with the photoaffinity atp analog 8-azidoadenosine 5'-triphosphate (8n3atp). two-dimensional tryptic maps of the four heterologous reca proteins demonstrated considerable structural conservation ...19883286605
nature of monocyclic beta-lactam antibiotic nocardicin a to beta-lactamases.nocardicin a is the antibiotic which was first found to possess a monocyclic beta-lactam ring. this antibiotic was inactivated by the cleavage of its beta-lactam ring. the direct spectrophotometric assay was applied to measure the rate of enzymatic hydrolysis of nocardicin a. nocardicin a was highly stable to both chromosomal and plasmid-mediated beta-lactamases. of the nine beta-lactam antibiotics including cefoxitin and cefuroxime, nocardicin a was the most stable to the beta-lactamases tested ...19883287105
a multicenter comparative study of cefotetan once daily and cefoxitin thrice daily for the treatment of infections of the skin and superficial soft tissue.to compare the effectiveness of cefotetan administered at 2 g once a day with cefoxitin at 1 or 2 g three times a day in the treatment of hospitalized patients with skin and superficial soft tissue infections, 194 patients from eight centers were enrolled in an open, randomized trial. most of the 104 evaluable patients in the cefotetan group and 50 in the cefoxitin group were young men with community-acquired, moderate or severe cellulitis, or abscesses of the upper and lower extremities caused ...19883287976
carumonam's in-vitro activity against gram-negative bacteria and its stability to their beta-lactamases.the in vitro activity of the novel monobactam carumonam (ro17-2301) was evaluated on 311 gram-negative clinical isolates in comparison to aztreonam, cefotaxime, ceftazidime, cefotetan and ceftriaxone. carumonam showed an antibacterial potency equal to or higher than any other reference compound; in particular it was the most effective against proteus indole positive and klebsiella sp. its antipseudomonal activity was comparable to that of ceftazidime and it showed, together with aztreonam, the h ...19883288365
intravesical migration of lippes loop with stone formation.a case is described of a 47-year old multipara, presenting with dysuria and frequency. urinalysis showed pus and proteus mirabilis infection, and radiography revealed the presence in the bladder of 2 stones joined together by a migrated and entrapped lippes loop, which had been inserted 14 years previously, after the birth of her 5th child.19883289679
circumcision and periurethral carriage of proteus mirabilis in boys.swabs were taken for culture from the periurethral area and urethral meatus in 124 uncircumcised and 60 circumcised boys. proteus mirabilis was grown from 28 (22.6%) swabs from uncircumcised boys and from only one (1.7%) swab from circumcised boys. this supports the idea that the prepuce may be the source of proteus urinary tract infection.19883291784
[dynamics of drug resistance in proteus mirabilis cultures 1970-1985].resistance of 669 clinical strains of proteus mirabilis to 18 chemotherapeutic drugs was studied in dynamics within 1970-1985. an increase in the number of cultures resistant to ampicillin and carbenicillin was noted while the number of cultures resistant to cephalosporines did not change. within the period from 1970 to 1975 there was observed a marked increase in the number of proteus strains resistant to aminoglycoside antibiotics. after that period their number gradually lowered and in 1985 r ...19883291802
proteus mirabilis urease. partial purification and inhibition by boric acid and boronic acids.urease was purified 800-fold and partially characterized from proteus mirabilis, the predominant microorganism associated with urinary stones. boric acid is a rapid reversible competitive inhibitor of urease. the ph-dependence of inhibition exhibited pka values of 6.25 and 9.3, where the latter value is probably due to the inherent pka of boric acid. three boronic acids also were shown to inhibit urease competitively.19883291857
the production of anorexigenic substances by intestinal flora.the role of intestinal flora in the production of anorexigenic substance was investigated. proteus mirabilis (p. mirabilis) and escherichia coli (e. coli) were found to produce an anorexigenic substance, while enterococcus faecalis (e. faecalis, type 1 and 2) and staphylococcus intermedius (s. intermedius) did not. the anorexigenic substance was purified and was detected as, a single though broad band by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). the specific activity ...19883291869
experience with antimicrobial combinations in urinary tract infections.four antimicrobial combinations were tried in the management of recurrent upper urinary tract infections: (i) rifampicin + trimethoprim (50 patients); (ii) amoxycillin + clavulanic acid (50 patients); (iii) ampicillin + sulbactam (10 patients); and (iv) pivampicillin + pivmecillinam (50 patients). the initial and final microbiological success rate was excellent with all four drug combinations; all were well tolerated. although monotherapy should be the rule in antimicrobial chemotherapy, there a ...19883292180
bacterial translocation in dianhydrodulcitol-treated mice.escherichia, proteus, klebsiella and streptococcus strains were isolated from mesenteric lymph nodes, spleens and livers of conventional mice treated with dianhydrodulcitol (dad), indicating that intestinal bacteria had appeared in organs usually containing no bacteria. the frequency of bacterial translocation showed direct relation to the dose of the drug and appeared simultaneously with the spleen atrophy caused by dad.19883293340
production of a phosphatidylglycerol-like substance by genital flora bacteria.bacteria contaminating amniotic fluid have anecdotally been shown to produce a phosphatidylglycerol-like substance, thus giving false positive tests for phosphatidylglycerol in vaginal pool fluid from patients with premature rupture of the membranes. isolates of escherichia coli, proteus mirabilis, acinetobacter calcoaceticus, klebsiella pneumoniae, staphylococcus aureus, coagulase-negative staphylococcus, streptococcus faecalis, and streptococcus agalactiae (group b streptococcus) were grown in ...19883293449
comparative in vitro activity of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone against hospital strains of gram-negative and -positive bacteria.the in vitro antibacterial activities of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone were compared against 330 gram-negative and gram-positive strains isolated from clinical specimens received at the king abdulaziz university hospital (kauh) in saudi arabia. the antibacterial susceptibility was determinated by stokes method and by the minimal inhibitory concentration (mic) using an agar dilution method. ceftazidime and ceftriaxone were the most active antibiotics, inhibiting ...19883293818
evaluation of the in vitro susceptibility of gram-negative bacilli to cefotaxime, over a period of 3 years. 19883293972
bacteriuria following extracorporeal shock wave lithotripsy of infection stones.extracorporeal shock wave lithotripsy effectively pulverizes infected (struvite) renal calculi. however, after treatment minute residual fragments that may harbor bacteria and cause persistent bacteriuria remain in the renal collecting system for months. we investigated prospectively the incidence of persistent proteus mirabilis bacteriuria after extracorporeal shock wave lithotripsy among 15 consecutive women with proteus mirabilis urinary tract infections and struvite calculi. all patients rec ...19883294439
adherence of urease-induced crystals to rat bladder epithelium following acute infection with different uropathogenic microorganisms.apart from urine supersaturation with respect to struvite and calcium phosphate, crystal retention is considered to be necessary for the formation of infection stones. this study was performed to investigate the role of the mucous coat in rat bladders in the adhesion of sterile urease-induced crystals and to determine to what extent the adhesion was influenced by infection. elimination of the mucous coat with 0.1 m hcl increased the adherence of crystals six times compared to that in bladders wi ...19883294448
re: considerations in the choice of sutures for wound closure of the genitourinary tract. 19883336087
septicemia from biliary tract infection.one hundred four strains of microorganisms were isolated from the blood in 76 episodes of septicemia originating from biliary tract infection. the 70 patients involved included 40 with acute cholecystitis without previous surgery, 17 with cholangitis following previous surgery, and 13 patients with malignant disease, with or without previous surgery. the most common organisms were escherichia coli, klebsiella, streptococcus, and proteus. various streptococci, most of them from group d, were invo ...19883341915
numerical analysis of electrophoretic protein patterns of providencia alcalifaciens strains from human faeces and veterinary specimens.twenty-five strains of providencia alcalifaciens from various countries have been characterized by one-dimensional sds-page of cellular proteins. they comprised 15 from human faeces, one from duck faeces, one from a guinea-pig eye and eight from unknown sources. also included, for reference purposes, were the type strains of three other providencia species. the protein patterns, which contained 45-50 discrete bands, were highly reproducible and were used as the basis for two numerical analyses. ...19883350783
interactions between antibacterial agents of the quinolone group and nitrofurantoin.plate diffusion tests showed that nitrofurantoin antagonized the activity of nalidixic acid, norfloxacin, ciprofloxacin and enoxacin against many gram-negative bacilli, including all proteus mirabilis and klebsiella aerogenes strains tested. no interaction was exhibited when nitrofurantoin and the newer quinolones were tested against pseudomonas aeruginosa, staphylococci or streptococci. antagonism was not reliably demonstrated in chessboard titrations, especially with the newer quinolones. cont ...19883356622
microbial keratitis associated with contaminated ocular medications.we studied seven cases of severe gram-negative microbial keratitis associated with the use of contaminated topical ocular medications. five cases involved pseudomonas aeruginosa, one involved serratia marcescens, and one involved proteus mirabilis. in each case the same organism was cultured from corneal scrapings and from the medication. either prednisolone acetate (one case) or timolol maleate (seven cases) was implicated in all instances.19883358428
a survey of clinical experience with ciprofloxacin, a new quinolone antimicrobial.ciprofloxacin is a new quinolone antimicrobial agent with activity against a broad spectrum of gram-negative and gram-positive organisms, including pseudomonas aeruginosa and methicillin-resistant strains of staphylococci. the efficacy and safety results of 80 clinical studies of the oral form of ciprofloxacin are reported. drug safety was assessed in 2236 courses in 2203 adult patients treated primarily in the united states. data from 1676 courses were suitable for analysis of drug efficacy. th ...19883360968
[interrelations between klebsiella ozaenae and other members of a microbial association in a pathological ozenous focus].the study of relationships between individual representatives of the microeubiosis at a pathological ozenous focus has shown that gram-positive microorganisms and opportunistic enterobacteria are not antagonistic to k. ozaenae which, in their turn, do not affect the growth of opportunistic enterobacteria and pseudomonas aeruginosa. the overwhelming majority of p. aeruginosa strains (92.9%) grown on a solid culture medium are characterized by a varying antagonistic activity with respect to k. oza ...19883364076
cell-mediated immunity assessed by skin testing (multitest). ii: correlation between responses from arm and back.in 60 healthy adult volunteers and 58 patients with gastrointestinal disease a test system (multitest) consisting of a plastic disposable multiple-puncture device capable of simultaneously applying seven delayed-type hypersensitivity antigens and a glycerin/saline diluent (negative control) was assessed. the multitest device was applied on both the inner side of the forearm and on the back for assessment of cell-mediated immunity (cmi). the antigens used were two toxoids, tetanus and diphtheria, ...19883364623
new plate medium for screening and presumptive identification of gram-negative urinary tract pathogens.a new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is described. the medium combines adonitol fermentation, phenylalanine deaminase, and beta-glucuronidase tests and allows the indole and cytochrome oxidase tests to be performed directly from the plates. high-level agreement with individual conventional tests was recorded in comparative studies with 504 cultures of gram-negative rods. there was 100% agreement, except for the providencia spp. i ...19883366875
kell blood group activity of gram-negative bacteria.to understand better the relationships between blood-group antigens and bacterial constituents, examples of 23 gram-negative bacteria (representing the 10 genera citrobacter, edwardsiella, enterobacter, escherichia, klebsiella, proteus, pseudomonas, salmonella, serratia, and shigella) were tested for the presence of kl-like antigens by hemagglutination-inhibition (hai) assays against both igg and igm anti-kl. saline-suspended whole organisms, cell-free culture media, and disrupted organisms were ...19883368935
[effectiveness of prodigiozan, methyluracil and levamisole in experimental infection after administration of non-steroidal anti-inflammatory drugs].the effect of nonsteroid antiinflammatory drugs (naid) such as voltaren, acetylsalicylic acid, indometacin and naproxen on the efficacy of immunostimulators in treatment of infections caused by proteus and staphylococcus was studied. it was shown that except naproxen the naid were capable of decreasing resistance to proteus infections. acetylsalicylic acid increased resistance to staphylococcal infections. the rest of the drugs did not change it. prodigiozan increased resistance to the infection ...19883377603
a computer-assisted structural analysis of regular polysaccharides on the basis of 13c-n.m.r. data.a computerised approach to the structural analysis of unbranched regular polysaccharides is described, which is based on an evaluation of the 13c-n.m.r. spectra for all possible primary structures within the additive scheme starting from the chemical shifts of the 13c resonances of the constituent monosaccharides and the average values of the glycosylation effects. the analysis reveals a structure (or structures), the evaluated spectrum of which resembles most closely that observed. the approach ...19883378242
[skin reactions with ubiquitous multiple antigens as a test of survival in patients with inoperable bronchogenic carcinoma].fifty-three patients suffering from inoperable, non-microcytoma, bronchogenic carcinoma in which a skin reaction with ubiquitous antigens (tetanus, diphtheria, streptococcus, tuberculin, proteus, tricophyton, candida) had been carried out were followed up to check on the possible relationship between skin reactivity and survival period. a significant correlation was found between the degree of positivity and duration of survival, with mean survival values greater than 69.1 weeks being observed i ...19883380311
cutaneous manifestations of the proteus syndrome.the proteus syndrome is a rare disorder in which the major manifestations are skeletal overgrowth, digital hypertrophy, exostoses of the skull, and hamartomatous tumors. numerous skin lesions also occur. we treated six individuals, all of whom had the features unique to this syndrome of marked hypertrophy of the skin of the soles. the palms were similarly involved in two patients. light microscopy of biopsy material from thickened areas of the soles showed elongation of the cytoplasm of the basa ...19883380758
a survey of the aerobic bacteria in the feces of captive raptors.feces of 47 captive raptors belonging to the order falconiformes or strigiformes were cultured for bacteria. gram-negative bacteria, which were cultured from the feces of 45 of the 47 raptors, were the most common isolates. a wide variety of species were identified, including a newly described genus (moellerella wisconsensis), two newly described species (escherichia fergusonii and proteus penneri), and a member of a newly described enteric group (cdc enteric group 41). additional organisms iden ...19883382380
characterization of urease from campylobacter pylori.campylobacter pylori, a suspected agent of gastritis and peptic ulceration, rapidly hydrolyzes urea. because urease serves as the basis of detection of the organism in gastric biopsies and may represent an important virulence factor, biochemical characteristics of the enzyme were determined. c. pylori was isolated from antral biopsies from 10 patients with complaints of abdominal pain or history of peptic ulcer disease. all isolates were urease positive, with an average rate of hydrolysis by cel ...19883384908
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...19883386529
ciprofloxacin: in vitro, experimental, and clinical evaluation.ciprofloxacin at a concentration of 2 micrograms/ml inhibited the growth of approximately 90% of 584 strains of aerobic bacteria isolated from cultures of blood drawn from septicemic patients. an increase in the inoculum size did not result in an increased mic, but serial passages through media containing ciprofloxacin at sub-mic levels increased the mic for escherichia coli, klebsiella pneumoniae, and proteus vulgaris. in experimental subcutaneous abscesses in the mouse model, ciprofloxacin was ...19883393781
recurrent and residual renal calculi in children.one hundred and thirteen children with non-metabolic urinary tract calculi were operated on between 1960 and 1983. one hundred were followed up for 3 to 17 years (mean 8.2). seventeen children (15%) developed recurrent calculi after complete clearance at the initial operation and in 13 of these the stones recurred within 5 years. proteus urinary infection was a constant feature of stone recurrence. five patients required re-operation for removal of the recurrent calculus. small residual caliceal ...19883395797
activity of cefotaxime and amikacin against 14,272 gram-negative bacteria from clinical samples in the period 1980 to 1985.the sensitivity to cefotaxime and amikacin of 14,272 gram-negative bacilli (enterobacteriaceae and non-fermenting gram-negative bacilli) isolated from clinical samples was studied during the period 1980 to 1985. the minimum inhibitory concentration (mic) was determined by means of diffusion in agar. strains were considered resistant to cefotaxime and amikacin if the mic values were greater than 16 mg/l and greater than 8 mg/l, respectively. the mic90 reached the critical value for cefotaxime in ...19883396470
cefotaxime combined with selective decontamination in long term intensive care unit patients. virtual absence of emergence of resistance.emergence of bacterial resistance to antimicrobial agents was studied during a period of 30 months of continuous use of parenteral cefotaxime combined with oral non-absorbable polymyxin e and tobramycin (selective decontamination) in a surgical intensive care unit (icu). no increase in drug-resistance micro-organisms was found. colonisation of the oropharyngeal cavity or intestine or both by strains resistant to polymyxin e occurred in 8% of patients (invariably proteus and morganella species). ...19883396487
possible regulation of cation-induced pinocytosis in amoeba proteus by phospholipase a.we have studied the effects of exogenous phospholipids and compounds which are known to alter the activity of phospholipase a (pla) on ca2+-dependent, na+-induced pinocytosis in amoeba proteus. the pla-inhibitors mepacrine, p-bromophenacyl bromide (pbpb) and rosenthal's inhibitor depressed pinocytosis. normal pinocytotic intensity was restored by the addition of ca2+ or picomolar concentrations of lysolecithin. very low concentrations of lysophospholipids and different molecular species of lecit ...19883396589
recurrent gram-negative bacteremia.recurrent gram-negative bacteremia is defined as two or more episodes of bacteremia occurring in the same patient with an infection-free interval between each episode. our purpose was to identify patients with recurrent gram-negative bacteremia in order to define possible risk factors for its recurrence. patients and19883400692
[treatment of staghorn calculi by percutaneous nephrolithotomy. apropos of 18 cases].this series involves 19 kidneys treated by the same operator. the predominance of women (14/18) and of proteus urinary infection (11) was identical to other published series. 13/19 calculi with complete staghorn calculi occupying the renal pelvis and the 3 calyceal groups. 7 patients had a past history of 15 lombotomies for staghorn calculus. pcln was carried out under fluoroscopic visualisation only, in 13 cases with one single channel, in 4 cases with two channels and in 3 cases with three cha ...19883411131
effect of circumcision status on periurethral bacterial flora during the first year of life.to determine whether bacterial colonization is a factor in the pathogenesis of urinary tract infection, we compared the periurethral bacterial flora of uncircumcised versus circumcised boys during the first year of life. intraurethral and circumferential glans cultures were obtained from 25 circumcised and 25 uncircumcised infants at 2 days, 2 weeks, 2 months, 4 months, 6 months, and 12 months of age. different children were used at each age (300 total). the types of bacteria and the total and s ...19883411387
ciprofloxacin activity in cyst fluid from polycystic kidneys.renal cyst infection in patients with polycystic kidney disease (pkd) is often unresponsive to standard antimicrobial therapy, in part because of the failure of most antibiotics to adequately penetrate cyst fluid. ciprofloxacin, a new quinolone antibiotic, possesses in vitro activity against most pathogens likely to be encountered in renal cyst infection. to study the potential usefulness of ciprofloxacin for the treatment of cyst infection, fluid from 70 cysts was obtained from seven patients w ...19883415205
[antiproteus activity of the serum of the blood of donors and of the agent "immunoglobulin"]. 19883420884
activity of e-3846, a new fluoroquinolone, in vitro and in experimental cystitis and pyelonephritis in rats.the in vitro antibacterial activity of e-3846, a new fluoroquinolone carboxylic acid derivative with a pyrrol ring substituent at position 7, was evaluated in comparison with norfloxacin and ciprofloxacin. e-3846 was more active than the reference quinolones against staphylococcus species, including methicillin-resistant strains. e-3846 was similar to ciprofloxacin and more active than norfloxacin against streptococcus (enterococcus) faecalis. in general, e-3846 was more active than norfloxacin ...19883134844
antibiotic prophylaxis for surgery after vesico-ureteral reflux in children. 19883135164
a regional survey of the resistance to beta-lactam antibiotics in clinical isolates of (facultative) aerobic micro-organisms.from 1980 to 1986 a regional survey was performed to investigate the susceptibility of beta-lactam antibiotics in clinical isolates of escherichia coli, proteus mirabilis, klebsiella pneumoniae, enterobacter cloacae and pseudomonas aeruginosa in 6 hospitals located in the southern part of the netherlands. each year 300 to 400 strains were tested. the susceptibility to representatives of the older and the newer beta-lactam antibiotics was determined by a microbroth dilution assay using an inoculu ...19883135169
comparison of in vitro activity of cefotaxime and desacetylcefotaxime alone and in combination against 320 gram-negative clinical isolates.the antimicrobial activity of cefotaxime and its intermediate metabolite, desacetylcefotaxime, against 320 gram-negative bacterial strains was analysed to investigate whether combination of the 2 substances led to increased bactericidal activity. the in vitro study of the minimum inhibitory concentration (mic) showed the combination to be more effective against escherichia coli, klebsiella spp., enterobacter spp. and proteus mirabilis, requiring less than or equal to 50% of the concentration of ...19883135171
further diagnostic thoughts about the elephant man.further evidence for a diagnosis of the elephant man's condition is reviewed. it is known that the elephant man had "mocassin" lesions, hyperostoses of the skull, and absence of café-au-lait spots, all of which are characteristic of proteus syndrome. recently, questions have been raised about his skeletal findings and their relevance to neurofibromatosis. however, other skeletal diagnoses have been entertained, including maffucci syndrome, paget's disease of bone, pyarthrosis, and fibrous dyspla ...19883135754
[demonstration of glycocalyx formation in vitro in citrobacter freundii, proteus mirabilis and planococcus sp.: the effect of polyosidases].three bacterial species of different origin, citrobacter freundii, proteus mirabilis and planococcus sp., formed glycocalyx in vitro and thereby showed that the phenomena does not only occur in vivo contrary to the opinion of many authors. the glycocalyx was produced in the attachment processes used or both extra- and intercellular attachment. the aggregates formed caused large errors in bacterial counts and led to questioning of the very principle of the counting procedures. the glycocalyx was ...19873135828
stones in the urinary bladder in children and young adults.bladder stones were diagnosed in 22 children and young adults at the children's hospital, boston, from 1969 to 1985. one half of the patients were less than 12 years old (mean, 11.9 years). one or more lithogenic factors were implicated in all but two. the most common causes were the presence of an intravesical foreign body, infection with proteus (a urea-splitting organism), exstrophy of the bladder, and the presence of intestinal mucosa in the urinary tract. the radiologic features of the ston ...19873492874
cloning of genes from members of the family enterobacteriaceae with mini-mu bacteriophage containing plasmid replicons.an in vivo cloning system that uses derivatives of the escherichia coli bacteriophage mu with plasmid replicons has been extended to five different species of the family enterobacteriaceae. mu and these mini-mu replicon elements were introduced into strains of e. coli, shigella flexneri, salmonella typhimurium, citrobacter freundii, and proteus mirabilis by infection, by transformation, or by conjugation with newly constructed broad-host-range plasmids containing insertions of these elements. ly ...19873542967
in vitro activity and beta-lactamase stability of a new penem, cgp 31608.the in vitro activity of cgp 31608, a new penem, against aerobic and anaerobic organisms was evaluated and compared with those of other beta-lactams. cgp 31608 inhibited escherichia coli, klebsiella pneumoniae, k. oxytoca, proteus mirabilis, citrobacter diversus, and salmonella, shigella, aeromonas, and yersinia spp. with mics for 50% of the strains (mic50s) of 2 to 4 micrograms/ml and mic90s of 4 micrograms/ml, compared with cefotaxime, ceftazidime, aztreonam, and imipenem mics of less than 0.2 ...19873496845
comparative activity of ceftizoxime against aminoglycoside-resistant aerobic gram-negative bacilli.the in vitro activity of ceftizoxime was compared with that of other beta-lactam antibiotics against 331 aminoglycoside (ag)-resistant clinical isolates. two hundred and six ag-resistant, beta-lactamase producing, r-plasmid harbouring enterobacteriaceae strains had mics ranging from 0.0125 to 0.063 mg/l. ag-resistant escherichia coli (36 strains) and klebsiella pneumoniae (19) had mic 90 values of 8 mg/l. proteus rettgeri and p. vulgaris as well as morganella morganii, resistant to several ags, ...19873497792
in vitro activity of cefixime and six other agents against nosocomial pathogens of the enterobacteriaceae family.cefixime, a broad-spectrum, orally active cephalosporin, was more active in vitro than ampicillin, cefaclor, cephalothin, and trimethoprim/sulfamethoxazole against 194 nosocomial pathogens of the family enterobacteriaceae. activity was especially good against klebsiella spp, proteus spp, serratia spp, and providencia stuartii. although gentamicin had equivalent or better activity against citrobacter spp, enterobacter spp, escherichia coli, and morganella morganii, all 23 of the gentamicin-resist ...19873498702
in vitro and in vivo evaluations of bmy-28100, a new oral cephalosporin.a new semisynthetic oral cephalosporin, bmy-28100, was evaluated for in vitro and in vivo antibacterial activities in comparison with cefaclor and cephalexin. bmy-28100 showed in vitro activity 3- and 10-fold more potent than that of cefaclor against staphylococcus aureus and streptococcus pneumoniae, respectively. bmy-28100 was slightly better than cefaclor and about 4 times more active than cephalexin against haemophilus influenzae and neisseria gonorrhoeae. escherichia coli, klebsiella pneumo ...19873500158
beta-lactamase of pseudomonas pseudomallei and its contribution to antibiotic resistance.beta-lactamase production was examined in nine strains of pseudomonas pseudomallei isolated from human, animal and environmental sources in thailand and hong kong. all produced the same weakly inducible, membrane associated chromosomal cephalosporinase, which had a molecular weight of 29,500 and an isoelectric point of 7.4-7.7. the enzyme resembled the cefuroximases of ps. cepacia and proteus vulgaris, but differed from the class i cephalosporinases typical of ps. aeruginosa and most enterobacte ...19873500161
in vivo determination of cell mediated immune response in diabetic patients using a multiple intradermal antigen dispenser.the in vivo cell mediated immune response using a multiple intradermal antigen dispenser (multitest) was evaluated in 99 diabetic patients (24 type i and 75 type ii) and in 50 age matched normal subjects. seven different antigens (tetanus, diphteria, streptococcus, tubercoline, candida, trichophyton, proteus and a glycerine control) were applied in the forearm and the induration for the antigens tested was measured 48 hours later. a score was calculated adding the arithmetic means obtained with ...19873500820
urinary pathogens and bacterial sensitivity in hospitalized urological patients based upon clinical aspects.for a total of 396 hospitalized urological patients with complicated and/or hospital-acquired urinary tract infections (uti) urinary pathogens with colony counts of 10(5)/ml or more were determined, several species were then subclassified by epidemiological markers. the minimal inhibitory concentrations (mic) were measured using the agar dilution method for seven penicillins and for four penicillin combinations, for six oral and 14 parenteral cefalosporins, for three older and five newer quinolo ...19873501901
[clinical laboratory approach for estimating effective administrative dose of ceftizoxime. observation from mic and ceftizoxime disc susceptibility test].in vitro activities of ceftizoxime (czx) against 328 clinical isolates were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. czx was highly active against escherichia coli, klebsiella pneumoniae, proteus mirabilis, and proteus vulgaris with mic values below 0.20 microgram/ml. it was also active against serratia marcescens and enterobacter aerogenes with mic85 of 3.13 micrograms/ml. czx was less active against staphylococcus aureus and staphylococcus epidermidis, sh ...19873474430
Displaying items 5601 - 5700 of 15180