Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| switches in fish myosin genes induced by environment temperature in muscle of the carp. | fish are cold blooded animals and their muscle function is expected to be greatly affected by environmental temperature. species that live in the antarctic ocean have evolved a different contractile system to fish that live in the tropical waters. in the case of antarctic fish they have a higher specific myofibrillar atpase activity but 'the trade off' seems to be a lower thermal stability. they are thus capable of a greater muscle power output at low temperatures but the lower thermal stability ... | 1992 | 1341032 |
| phototransduction in cones as examined in excised membrane patch. | phototransduction mechanisms have been so far investigated mostly in rods, whereas those in cones are much less known. in the present experiment, we investigated phototransduction mechanisms in inside-out patches excised from cone outer segments of the carp. cyclic gmp-activated channels on the patch became light-sensitive when both gtp and mg2+ were supplied by perfusion. when the channels were activated by a hydrolysis-resistant analogue of cgmp, activities were not suppressed by light even th ... | 1992 | 1331581 |
| peptidergic regulation of muscle movements in a prosobranch mollusc, rapana thomasiana. | peptidergic regulation of the contraction of radula muscles and the heart beat in a prosobranch mollusc, rapana thomasiana was investigated. in the radula muscles, fmrfamide-related peptides were suggested to act on presynaptic sites to enhance their contraction elicited by nerve stimulation, possibly by increasing the release of a main transmitter. a catch-relaxing peptide (carp) was demonstrated to depress the contraction by acting on postsynaptic sites. in the heart, fmrfamide enhanced the am ... | 1992 | 1299108 |
| risk factors for kidney cancer in new south wales, australia. ii. urologic disease, hypertension, obesity, and hormonal factors. | in a population-based case-control study of kidney cancer in new south wales, australia, data from structured interviews with 489 cases of renal cell cancer (rcc) and 147 cases of renal pelvic cancer (carp) diagnosed in 1989 and 1990, and 523 controls from the electoral rolls, confirmed the link between obesity and rcc. in addition, regular consumption of 'diet' pills independently increased the risk for this cancer. a diagnosis of hypertension at least two years before interview raised the risk ... | 1992 | 1617119 |
| predicted secondary and tertiary structures of carp gamma-crystallins with high methionine content: role of methionine residues in the protein stability. | a systematic structural comparison of several carp gamma-crystallins with high methionine contents was made by the secondary-structure prediction together with computer model-building based on the established x-ray structure of calf gamma-ii crystallin. the overall surface hydrophilicity profile and the distribution of helices, beta-sheets, and beta-turns along the polypeptide chains are very similar among these carp gamma-crystallins. in addition, their general polypeptide packing is close to t ... | 1992 | 1429521 |
| the effect of malathion, diazinon, and various concentrations of zinc, copper, nickel, lead, iron, and mercury on fish. | acute and chronic toxicity tests for malathion, diazinon, copper (cu), mercury (hg), lead (pb), zinc (zn), nickel (ni), and iron (fe) were conducted. mortalities of barilius vagra and cyprinus carpio (common carp) were variable but lc50-96 hr were similar for pesticides. adult b. vagra seem to be more sensitive to malathion than juvenile carp. both juvenile carp and adult b. vagra were extremely sensitive to diazinon. long-term exposure to pesticides modified morphology and behavior. the lc50-96 ... | 1992 | 1384613 |
| push-pull modulation of ganglion cell responses of carp retina by amacrine cells. | the responses of a ganglion and an amacrine cell were recorded simultaneously in the carp retina. sinusoidal current injected into amacrine cells modulated ganglion cell discharges either in phase (excitation) or in opposite phase (inhibition). on-center ganglion cells received excitatory inputs and off-center ganglion cells received inhibitory inputs from on-center amacrine cells. they received inputs of opposite polarity from off-center amacrine cells. namely, inputs from on-center and off-cen ... | 1992 | 1407715 |
| drinking behaviour in sea water and fresh water teleosts, the role of the renin-angiotensin system. | basal drinking rate and responses to administered angiotensin were examined in 12 species of fish. the responses of representative euryhaline, stenohaline marine and fresh water species to pharmacological manipulation of endogenous renin-angiotensin system (ras) activity were also investigated.basal drinking rates were consistently low in stenohaline and euryhaline fresh water fish, and all species examined showed an increased imbibition in response to administered angiotensin. marine fish drank ... | 1992 | 24214213 |
| neuropeptides regulate the cardiac activity of a prosobranch mollusc, rapana thomasiana. | involvement of neuropeptides in the regulation of cardiac activity in a prosobranch mollusc, rapana thomasiana, was studied physiologically as well as immunohistochemically. a catch-relaxing peptide (carp) showed strong inhibitory effects on the heart with a lower threshold than acetylcholine. the action of carp was in contrast to that of another neuropeptide, fmrfamide, which has previously been shown to enhance the heart beat. benzoquinonium blocked the effects of acetylcholine and stimulation ... | 1992 | 1423492 |
| effects of polyoxyethylene (20) sorbitan monooleate on the acute toxicity of linear alkylbenzenesulfonate (c12las) to fish. | effects of polyoxyethylene (20) sorbitan monooleate (smoe20) on the acute toxicity of linear alkylbenzenesulfonate (c12las) to fish were investigated in red killifish (orizias latipes) and carp (cyprinus carpio). by adding polyoxyethylene sorbitan ester to c12las solution, the acute toxicity of c12las decreased. as a decreasing toxic effect of these nonionic surfactants, the depression of gill damage was histopathologically observed. no significant hematological and blood biochemical differences ... | 1992 | 1385076 |
| the effects of atp, inorganic phosphate, protons, and lactate on isolated myofibrillar atpase activity. | the purpose of this study was to examine the effects of lactate, protons, inorganic phosphate, and atp on myofibrillar atpase activity. myofibrils were isolated from carp (cyprinius carpio l.) fast-twitch white muscle, and myofibrillar atpase activities were assessed under maximal activating calcium levels (pca 4.0) at 10 degrees c in reaction media containing metabolic profiles similar to those seen in fatiguing muscles. the ca(2+)-activated atpase activity was assessed by an atp regenerating a ... | 1992 | 1335356 |
| changes in retinal projections and ganglion cell morphology after unilateral enucleation in the common carp. | changes in retinal projections and ganglion cell morphology were studied in one-eyed individuals of the common carp, cyprinus carpio, which were enucleated at a juvenile stage (within 6 months after hatching) and kept for 18 months after the operation. gross examination of the brains showed a marked atrophy of the contralateral optic tectum and a fine attenuated optic tract ipsilateral to the remaining eye. all retinal recipient areas were bilateral, but numerous projections were heavier contral ... | 1992 | 1450895 |
| predator-induced phenotypical change in body morphology in crucian carp. | in a field experiment where the presence or absence of piscivorous pike (esox lucius) in ponds was manipulated, the morphology of crucian carp (carassius carassius) diverged, such that individuals became deeper bodied in pond sections with pike. a laboratory experiment confirmed that the presence of this predator induced a change in body morphology in the carp. estimation of prey vulnerability to predation by pike, a gape-limited predator, revealed that this increase in body depth resulted in cr ... | 1992 | 17778362 |
| summertime and early autumn activity of some enzymes in the carbohydrate and fatty acid metabolism of the crucian carp. | in june, july, and september the activities of five enzymes involved in the carbohydrate and fatty acid metabolism, namely phosphofructokinase (pfk), lactate dehydrogenase (ldh), citrate synthase (cs), cytochrome oxidase (cyt ox) and 3-hydroxy-acyl-coa dehydrogenase (had), were measured in the heart, liver, red muscle, white muscle, and gill lamellae of crucian carp (carassius carassius). ldh activity was measured in both reducing (ldhr) and oxidizing (ldho) directions.the pfk activity in red an ... | 1992 | 24213818 |
| maturational gonadotropin from the african catfish, clarias gariepinus: purification, characterization, localization, and biological activity. | a gonadotropic hormone of the african catfish, clarias gariepinus, was was purified and chemically characterized. its biological activity was tested and its localization in the gonadotropic cells of the pituitary demonstrated. an ethanolic extract of 500 pituitaries of adult male and female african catfish was subjected to ion-exchange chromatography on de-52. the 31- to 38-kda fraction was further purified on sephadex g-75. on rphplc over an ods 120t column two major components appeared as sing ... | 1992 | 1426937 |
| [the characteristics of carbohydrate transport in the intestines of fresh-water fishes]. | the maximal level of carbohydrate transport was found in the proximal portion of the intestine and in the distal one in the bream and carp. after 48-hr starvation the carbohydrate transport was decreased along the whole length of the intestine. anoxia and some inhibiting agents changed the intensity of glucose and galactose transport in the fish intestine. | 1992 | 1335912 |
| quaternary structure and the geminate recombination of carp hemoglobin with methylisocyanide. | the kinetics of geminate recombination were studied for the methylisocyanide derivative of carp hemoglobin. carp hemoglobin is of interest because it has been established that the fully liganded form switches between a high affinity r state at ph 9 and a low affinity t state at ph 6 in the presence of ihp. geminate recombination was observed on both the picosecond and the nanosecond time scales under all conditions; however, only a small variation is observed in the rates and the yields of gemin ... | 1992 | 2393395 |
| deletion 11q23-->qter (jacobsen syndrome). report of three new patients. | three unrelated patients with de novo del 11q23-->qter are reported. clinical features included growth and mental retardation, hypotonia, trigonocephaly, facial dysmorphism with hypertelorism, epicanthal folds, abnormally shaped palpebral fissures, eye globe malformations, depressed nasal bridge, "carp-shaped" mouth, highly arched palate, low set and malformed ears. one patient had congenital heart defect, and reduced platelet count. this syndrome, originally reported by jacobsen, is now corrobo ... | 1992 | 1296516 |
| [purification and characterization of hec toxin produced by aeromonas hydrophila]. | an extracellular toxin produced by aeromonas hydrophila from cultured crucian carp with septicemia was detected. the toxin was purified by ammonium sulfate precipitation, deae-cellulose chromatography and sephadex g-100 gel filtration. the factor was a single polypeptide with a molecular weight of 52.5kd determined by sds-page. the heat-stable toxin possesses hemolytic, enterotoxic and cytolytic activities. the hemolytic activity on human erythrocytes was 3.81 x 10(3) hu/mg, cd50 for vero cell w ... | 1992 | 1299032 |
| chemical identification of catfish growth hormone and prolactin. | isolation and primary structure of growth hormone (gh) and prolactin (prl) from the pituitary gland of catfish (ictalurus punctatus) are described. alkaline extract of the pituitary glands was fractionated by gel filtration on sephadex g-75, ion-exchange chromatography on deae-cellulose, and reversed-phase high-performance liquid chromatography on octadecyl silica ods. catfish gh and prl were identified by western blotting with antisera against chum salmon gh and prl. the catfish gh consists of ... | 1992 | 1308206 |
| fish transgene expression by direct injection into fish muscle. | the ability of a promoter sequence to drive expression of a reporter gene can be determined by direct injection of copies of the cloned sequence into fish muscle, followed by biopsy of muscle from the site of injection. we describe a set of experiments in which copies of the constructs fv1 and fv2, both comprising a carp beta-actin promoter sequence spliced to the bacterial reporter gene cat, were injected into the muscle of tilapia fish )oreochromis niloticus) of between 5 and 8 cm body length. ... | 1992 | 1308819 |
| m-phase-specific histone h1 kinase in fish oocytes. purification, components and biochemical properties. | we demonstrate, for the first time in fish, that a ca(2+)-independent and cyclic-nucleotide-independent histone h1 kinase activity oscillates according to the cell cycle of the oocyte, peaking at the first and the second meiotic metaphase with a transient drop between them. the kinase, m-phase-specific histone h1 kinase (m-h1k), was purified from mature carp oocytes by using two exogenous substrates for assaying its activity: histone h1 and a synthetic peptide (sp peptide, kkaakspkkakk) containi ... | 1992 | 1315270 |
| low concentrations of neuroactive peptides modulate cholinergic transmission and cyclic amp levels in helix aspersa. | 1. fmrfamide and the catch relaxing peptide (carp) at 0.01-0.1 nm modulate acetylcholine (ach) induced currents of identified neurons of helix aspersa, but have no direct effect on membrane current and conductivity. 2. both fmrfamide and carp noncompetitively inhibit ach cl- responses while having either no effect or increasing ach na+/k+ response. 3. the inhibitory effect of fmrfamide and carp on the ach cl- response was eliminated following pretreatment with forskolin (20 microm), an activator ... | 1992 | 1338559 |
| electroporation: a method for transferring genes into the gametes of zebrafish (brachydanio rerio), channel catfish (ictalurus punctatus), and common carp (cyprinus carpio). | recombinant plasmids containing the rous sarcoma virus long-terminal repeat (rsvltr) promoter linked to either rainbow trout (oncorhyncus mykiss) growth hormone 1 (rtgh1) or growth hormone 2 (rtgh2) cdna were linearized and introduced into the fertilized eggs of zebrafish (brachydanio rerio), channel catfish (ictalurus punctatus), and common carp (cyprinus carpio) by both electroporation and microinjection. the latter two species had these rainbow trout constructs (rsvltr-rtgh1cdna or rsvltr-rtg ... | 1992 | 1339228 |
| oral absorption of biologically active insulin in common carp (cyprinus carpio l.). | 1. bovine insulin dissolved in 0.05 m deoxycholic acid was absorbed through oral intubation in fish weighing 200-300 g. 2. the peak of absorption appeared in all fish, 30-45 min after intubation and was followed by a gradual decrease. the extent of absorption was extremely variable, with up to a 20-fold difference between individuals. no detectable insulin was found in fish intubated with vehicle. 3. the absorbed hormone retained 17-88% of its lipogenic bioactivity in vitro. the absorbed insulin ... | 1992 | 1347729 |
| immunochemical characterization of rat testicular asparagine synthetase. | we studied immunochemical properties of rat testicular asparagine synthetase. western blot analysis of testis extract with polyclonal antibody raised against purified asparagine synthetase revealed an immunoreactive band at 62 kda. the pancreas, brain, thymus, and spleen also showed 62-kda bands. the intensities of these bands were roughly proportional to the specific activities of the enzyme in these tissues. the antibody showed some degree of cross-reactivity to asparagine synthetases from hum ... | 1992 | 1349469 |
| xenobiotic-metabolizing enzyme systems in test fish--iv. comparative studies of liver microsomal and cytosolic hydrolases. | 1. the initial slopes of the substrate-activity curves of several hydrolases were determined in the microsomal and cytosolic fractions of the liver of several fish recommended by oecd for the regulatory testing of chemicals. 2. inter-species differences ranged within a factor of 7-17 for the esterases and reached a factor of 60 for the amidase. guppy and carp appeared endowed with hydrolase activities which, overall, are much higher than zebra fish, trout and golden orfe. 3. the comparison with ... | 1992 | 1350956 |
| gene expression following transfection of fish cells. | various genes containing different transcriptional regulatory elements (tre) and the bacterial marker gene coding for chloramphenicol acetyl transferase were transfected into several fish cell lines to evaluate the efficiency of expression in comparison with mammalian cells. the cmv and rsv tre were the most efficient non-inducible promoters in directing reporter gene expression. rsv and cmv appeared of similar potency in a stable fish cell line. the human hsp-70 promoter showed high potency in ... | 1992 | 1369156 |
| different sensitivity of the sarcoplasmic reticulum ca(2+)-atpase enzyme to fluorescein-isothiocyanate in rabbit and carp muscles. | 1. carp and rabbit sarcoplasmatic reticulum ca(2+)-atpase enzymes were compared with respect to their sensitivity to fitc labelling. 2. the carp enzyme showed much lower sensitivity to fitc in the ca(2+)-mg2+ activated atpase activity. fifty percent inhibition was observed at 20 microm labelling fitc concentration; in rabbit enzyme this inhibition was already achieved at 2 microm fitc. 3. the tryptic cleavage products of the carp enzyme identified with immunoblot analysis as well as with fitc fl ... | 1992 | 1388116 |
| malathion induced changes in the optomotor behavior of an indian carp, labeo rohita. | 1992 | 1421850 | |
| distribution and coexistence of urotensin i and urotensin ii peptides in the cerebral ganglia of aplysia californica. | urotensin i (ui) and urotensin ii (uii) were demonstrated in the cerebral ganglia of aplysia californica by applying immunocytochemical and radioimmunoassay procedures. sequential analysis of adjacent sections of the cerebral ganglia of aplysia demonstrated that the ui-immunoreactive (ui-ir) neurons of the f cluster of the cerebral ganglia also contained uii immunoreactivity (uii-ir). both ui-ir and uii-ir were also observed in a cuff-like arrangement of fibers surrounding the proximal portion o ... | 1992 | 1437712 |
| retinoic acid enhances the secretion of plasminogen from cultured rat microglia. | to determine the amount of plasminogen in microglial conditioned medium, a highly sensitive and specific enzyme-linked immunosorbent assay (elisa) for rat plasminogen was established. weak cross-reactivity with human serum plasminogen was observed, while no reactivity was detected with frog and carp plasminogen. the specificity of the immunosorbent assay was confirmed by western blotting. the secretion of plasminogen into the microglial culture medium was quantified by using the established elis ... | 1992 | 1459246 |
| interstitial deletion of the long arm of chromosome 11: report of a case and review of the literature. | a 12-month-old female infant with developmental delay, growth retardation, and dysmorphic features including dolichocephaly, telecanthus, ptosis, flat nasal bridge, anteverted nares, high-arched palate, carp-shaped mouth, micro-retrognathia, and low-set and posteriorly rotated ears was found to have an interstitial deletion of chromosome 11 involving bands q14-q22. immunoblot analysis of her fibroblasts revealed a normal amount of mitochondrial acetoacetyl-coenzyme a thiolase, of which gene locu ... | 1992 | 1472705 |
| isolation and characterization of hypothalamic growth-hormone releasing factor from common carp, cyprinus carpio. | a growth hormone-releasing factor (grf)-like peptide was isolated from the hypothalamus of common carp, cyprinus carpio, by acid extraction, gel filtration chromatography, immunoaffinity chromatography using antiserum directed against rat grf, and multiple steps of hplc using octadecyl columns. based on edman degradation and peptide mapping, this teleost grf was established to be a 45-residue peptide with the following primary structure: his-ala-asp-gly-met-phe-asn-lys-ala-tyr-arg-lys-ala-leu-gl ... | 1992 | 1475012 |
| five confirmed human cases of gnathostomiasis nipponica recently found in northern japan. | five confirmed human cases of gnathostomiasis nipponica exhibiting creeping eruption and itching were found sporadically from the autumn of 1991 to the winter of 1992 in the northern region of the mainland of japan. in all cases, a causative gnathostome with 3 transverse rows of hooklets on the head bulb was detected in biopsied skin. the morphological characteristics agreed with the advanced third-stage larvae of gnathostoma nipponicum. within a few weeks before development of symptoms, all pat ... | 1992 | 1491291 |
| [planar charged clusters--structural invariants of the gamma-crystallin family of the crystalline lens: functional role and evolutionary conservatism]. | plane charge clusters from the calf eye lens protein gamma-crystallin are considered. the clusters consist of four to six side chain charged groups with interatomic distances in ionic pairs from 4 to 7 a. the charge clusters appear to decrease the hydrophilic potential of the molecular surface which maintains the transparent refracting lens medium of vertebrates with a very high protein concentration. it is shown that the charge pattern for different gene products of one species is conservative ... | 1992 | 1491683 |
| purification and characterization of cathepsin h from hepatopancreas of carp cyprinus carpio. | 1. cathepsin h was purified about 5400-fold from hepatopancreas of carp (cyprinus carpio) by the method involving ammonium sulfate fractionation, and chromatography on s-sepharose, deae-sephacel, ultrogel aca54, concanavalin a-sepharose 4b and gpc on protein-pak 125. 2. the purified cathepsin h gave a single protein band on analytical-page, but migrated as two bands of 27,000 and 23,000 mol. wt on sds-page. 3. cathepsin h had a ph and temperature optimum of 6.5 and 45 degrees c using arg-mca as ... | 1992 | 1499287 |
| the cellular proliferative response, humoral antibody response, and cross reactivity studies of tetrahymena pyriformis with ichthyophthirius multifiliis in juvenile carp (cyprinus carpio l.). | the immune response of 10-12-week-old juvenile carp to t. pyriformis [ccap 1630/w, 1939 (w)] was investigated. the humoral antibody response following one and two intraperitoneal injections of whole live t. pyriformis (protein concentration of 25 micrograms/g body weight), separated by an interval of 4 weeks, was monitored over 12-16 weeks. peak antibody titres were detected 6 weeks following antigen administration. antibody titres were elevated following the second injection, and relatively hig ... | 1992 | 1505691 |
| demonstration of two forms of vitellogenin in serum of estradiol-17 beta-treated indian major carp, labeo rohita. | vitellogenin (vg) synthesis was induced in the male and non-vitellogenic female rohu, the indian major carp, by estradiol-17 beta(e2) where effect was more in female. a crude preparation of vg was isolated in the second peak after gel filtration on ultrogel aca 34 from the sera of vitellogenic female rohu and e2-treated male and female rohu. estimation of alkali-labile phosphorus was shown to be used as an index of vg. native-page analysis has revealed the presence of two forms of vg (vg1: 430,0 ... | 1992 | 1506024 |
| the role of elongation factors in protein synthesis rate variation in white teleost muscle. | protein synthesis-stimulating activity was assayed in the cytosolic fraction of white muscle from teleost fish (rainbow trout, carp) and of rat liver. in vitro protein synthesis-stimulating activity in the cytosolic fraction is reduced by food deprivation. the addition of elongation factors ef1, ef2, or ef1 + ef2 compensates for the starvation-induced loss of protein synthesis-stimulating activity in trout muscle cytosol. the action of ef2 is stronger than that of ef1 in this respect. however, e ... | 1992 | 1506491 |
| clinostomum complanatum (trematoda: clinostomatidae) in five new fish hosts in japan. | metacercariae of clinostomum complanatum were found in six species of natural freshwater fishes collected at koyama pond in tottori city of tottori prefecture, japan. five of these fish species are reported here as new records for second intermediate hosts of c. complanatum in japan: the silver crucian carp (carassius gibelio langsdorfi), the deepbodied crucian carp (carassius cuvieri), the carp (cyprinus carpio), the topmouth gudgeon (pseudorasbora parva) and the rose bitterling (rhodeus ocelat ... | 1992 | 1512883 |
| ontogenetic and comparative analysis of ldh isozymes in the three species of indian major carp. | expression patterns of lactate dehydrogenase (ldh) isozyme were investigated in embryonic and post embryonic stages of labeo rohita, cirrhinus mrigala and catla catla using starch gel electrophoresis. species specific and differential enzyme locus (gene) expression patterns were found in ldh up to 18th hr of study. the isozyme up to 36th hr after fertilization seemed to be completely active and showed electrophoretic patterns very similar to those of the adults. comparative analysis of the isozy ... | 1992 | 1521871 |
| a monoclonal antibody that recognizes different conformational states of skeletal muscle troponin c and other calcium binding proteins. | skeletal muscle troponin c contains four ca(2+)-binding sites, two with a high affinity for ca2+ that also bind mg2+ competitively (ca2+/mg2+ sites) and two sites of lower affinity that are specific for ca2+. we have characterized a monoclonal antibody (b9d9) that was produced against rabbit skeletal troponin c. the binding of this antibody to rabbit skeletal troponin c is sensitive to the binding of ca2+. increasing the ca2+ concentration produces a decrease in the amount of antibody bound with ... | 1992 | 1527217 |
| sensitivity to maturation-inducing steroids and gonadotropin in the oocytes of blue gourami trichogaster trichopterus (anabantidae, pallas, 1770). | this study examined in vitro the effect of 17 alpha-hydroxyprogesterone (17-p), 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20-p), and human chorionic gonadotropin (hcg) on vitellogenesis and the induction of germinal vesicle breakdown (gvbd) in oocytes of the female trichogaster trichopterus (pallas, 1770) after various in vivo treatments: (a) in nonreproductive condition; (b) in reproductive condition together with males; (c) injected with hcg; (d) injected with extract of carp gonadotropin ... | 1992 | 1577246 |
| structure of the mitochondrial urfa6l gene and trna(lys) gene from carp. | the carp mitochondrial urfa6l gene consists of 165 base pairs. the overall structural organization of the gene is very similar to that of the xenopus urfa6l gene. their nucleotide sequences exhibit 68% homology. the carp urfa6l gene encodes a protein of 54 amino acids. the amino acid composition of the protein is unusual because almost half of the residues consist of 5 hydrophobic amino acids (proline, tryptophan, leucine, isoleucine and tyrosine). a comparison between the amino acid sequences o ... | 1992 | 1580999 |
| study of precorneal tear film thickness and structure by interferometry and confocal microscopy. | past measurements of precorneal tear film thickness in rabbits and humans gave values of 4-7 microns. these have been the accepted values. however, measurements presented in this report are much higher. earlier techniques may have changed film thickness or not have fully included the layer containing mucus. little is known about the mucus component. thickness was measured in freshly killed animals of 10 species using two independent optical techniques. coherent light was reflected from eyes and ... | 1992 | 1582804 |
| [the therapy of swimbladder inflammation (renicola sphaerosporosis) of carp]. | in pond experiments the efficiency of different chemotherapy variants against swimbladder inflammation (sbi) was proved. only by feeding of fumagillin, specifically affecting myxosporidians, the infection of kidney with sphaerospora renicola as well as the force of sbi could be reduced. the chemotherapy with metronidazole remained inefficacious, whereas the permanent feeding with methylene blue prevented only the infection with bothriocephalus acheilognathi and probably lowered insignificantly t ... | 1992 | 1610018 |
| the laboratory maintenance of sanguinicola inermis plehn, 1905 (digenea: sanguinicolidae). | methodology is described for the laboratory maintenance of the life-cycle of sanguinicola inermis, using common carp (cyprinus carpio) as the definitive host and lymnaea peregra as the intermediate host. lymnaea auricularia was also infected but is not considered to be a suitable laboratory host. tench (tinca tinca) were susceptible to infection at high cercarial doses but subsequent infection rates were low. goldfish (carassius auratus) and lymnaea stagnalis were refractory to laboratory infect ... | 1992 | 1614729 |
| [histochemical investigations on the localization of acetylcholinesterase in the kidney of selected vertebrates]. | the light and electron microscopical localization of ache activity in the kidney of selected vertebrates was studied using the method of karnovsky and roots (1964) for light microscopy and the modification of the koelle and friedenwald's technique according to tsuji (1974, 1984) for electron microscopy. ache activity could be demonstrated light microscopically mainly within the glomeruli of some mammals (golden hamster, mouse, rat) and non-mammalian vertebrates (carp, frog). no activity was foun ... | 1992 | 1642103 |
| purification and characterization of maturation-promoting factor in fish. | maturation-promoting factor (mpf) activity has been demonstrated for the first time in fish oocytes. we purified mpf from a 100,000g supernatant of crushed, naturally spawned carp oocytes using four chromatography columns: q-sepharose fast-flow, p13suc1-affinity sepharose, mono s, and superose 12. the final preparation was purified over 1000-fold with a recovery of about 1%. on superose 12, mpf eluted as a single peak with an apparent molecular weight of 100 kda. sds-page analysis of the active ... | 1992 | 1728595 |
| purification and characterization of a calsequestrin-like calcium-binding protein from carp (cyprinus carpio) sarcoplasmic reticulum. | 1. a calsequestrin-like calcium-binding protein was purified from carp sarcoplasmic reticulum by column chromatographies using deae-cellulose and butyl-toyopearl 650s. 2. the mol. wt was estimated to be 50 kda, which was larger than that of rabbit calsequestrin (42 kda). 3. carp calsequestrin-like protein bound ca2+ with a higher affinity (apparent kd = 400 microm) and lower capacity (25 mol/mol) compared with rabbit calsequestrin (1 mm and 40-50 mol/mol, respectively). 4. anti-carp calsequestri ... | 1991 | 1769203 |
| effect of proteases on the activity of enolase from muscle of carp (cyprinus carpio) and pig. | the treatment of enolase from pig and carp (cyprinus carpio) with proteases resulted in a decrease of enzymatic activity, which depended on the kind of protease used. the most active were trypsin and subtilisin. substrate and magnesium ions protected enolase against inactivation. the enolase from pig muscle was much more resistant to protease action than this enzyme from carp muscle. some differences in the structure between the two enolases are suggested. | 1991 | 1796695 |
| development of khawia sinensis hsü, 1935 (cestoda: caryophyllidea) in the fish host. | the development of the tapeworm khawia sinensis has been observed up to the stage of sexually mature parasites releasing eggs in an experimentally infected definitive host (cyprinus carpio) at 15-16 degrees c. juvenile, maturing, adult and gravid tapeworms were found 2-12, 19-24, 36-62, and 78-91 days post infection, respectively. no apparent size difference between tapeworms from carp kept at 20-22 degrees c, examined 1-16 days post infection, and those from 15-16 degrees c was observed. in nat ... | 1991 | 1808032 |
| [elaboration of a dried-salted and smoked product using aquaculture species]. | the purpose of this work was to adapt the procedure developed at the instituto nacional de la nutrición salvador zubirán (innsz) for the conservation of the sardine to aquaculture species such as carp (ciprinus carpio) and tilapia (tilapia sp). the mixtures were designed on the basis of the chemical score method. the preparation of the patties included different fish presentations, and two methods were evaluated in order to obtain fish pulp. in the formulation of the final product, defatted soy, ... | 1991 | 1824516 |
| [trematodiases in tataria]. | high opisthorchiasis morbidity among local population and domestic animals was recorded in 1966 in alekseevskiĭ district of tataria. repeated screenings performed in 1989 revealed besides the above disease cases of pseudoamphistomiasis. 183 subjects have been examined, 90 of them (40.9%) revealed hepatobiliary system damages. bile analysis performed in 61 patients revealed lambliasis in 1 case, opisthorchiasis in 3 cases and pseudoamphistomiasis in 31 cases. metacercaria of the above trematode w ... | 1991 | 1829788 |
| carp erythrodermatitis (ce) due to an aeromonas hydrophila infection. casuistic and experimental results. | in november 1987 high losses of carp (cyprinus carpio) with the main symptom of skin ulcera were observed in a farm in northern greece. sixty-six isolates of bacteria, characterized mainly as aeromonas hydrophila or pseudomonas spp. could be isolated from lesions of diseased fish. transmission experiments with these isolates using mirror carp showed that aeromonas hydrophila strains induced identical clinical and pathological pictures after intra- or subcutaneous injection. extracts of these aer ... | 1991 | 1830440 |
| identification of immunoreactive atrial natriuretic peptide in bulbus arteriosus of freshwater teleostean fish. | 1. the presence and partial characterization of immunoreactive atrial natriuretic peptide (ir-anp) in the bulbus arteriosus were investigated using high performance liquid chromatography (hplc) and radioimmunoassay (ria) in several freshwater teleostean fish (catfish, silver crusian carp, snakehead, and rice eel). 2. the level of ir-anp in the bulbus arteriosus was comparable with that in the atria at about 5-15 pg/mg, 6-13 pg/mg wet wt, respectively, but was 2-4-fold higher than that in the ven ... | 1991 | 1839978 |
| studies on rod horizontal cell s-potential in dependence of the dark/light adapted state: a comparative study in cyprinus carpio and scyliorhinus canicula retinas. | changes in light sensitivity, response time-course and electrical coupling of rod driven horizontal cells (rhc) were examined in the dark and light adapted states by intracellular recording. comparative analyses were made of two fishes from different photic environments. rhc of cyprinus carpio and external horizontal cells (ehc) of scyliorhinus canicula retinas showed similar changes of their electrical responses to light stimuli when the dark-adapted retina was light-adapted: a decrease in sens ... | 1991 | 1843752 |
| calcium channels from cyprinus carpio skeletal muscle. | the complete amino acid sequence of the l-type calcium channel alpha 1 subunit from the carp (cyprinus carpio) white skeletal muscle was deduced by cdna cloning and sequence analysis. the open reading frame encodes 1852 amino acids (mr 210,060). a 155-amino acid cooh-terminal sequence (after the fourth internal repeat) is evolutionarily preserved (90% homology) and may represent an important functional domain of l-type calcium channels. the photolabeled, membrane-bound, and purified carp alpha 1 ... | 1991 | 1846962 |
| [the immunological status of cyprinus carpio l. in experimental mycotoxicosis]. | some humoral factors of nonspecific resistance have been determined in carp yearlings in 28 days after intraperitoneal administration of fungi culture filtrate (i), conidia (ii), micellar extract (iii) of the fungus aspergillus fumigatus fres., physiological solution (control). the toxicogenic and immunogenic effect of the fungus has been shown in i and ii variants of the experiment respectively. the injection of fungus micella did not have any influence on the studied factors. the account of sp ... | 1991 | 1859857 |
| absorption of bioactive human growth hormone after oral administration in the common carp (cyprinus carpio) and its enhancement by deoxycholate. | recombinant human growth hormone was administered orally to carp and serum levels of absorbed bioactive hormone were investigated using a highly sensitive nb2 rat lymphoma cell bioassay and radioimmunoassay. serum levels of bioactive hgh reached maximum values 30 min after oral intubation and then gradually decreased. co-administration of the hormone with deoxycholate to fasted carp resulted in up to a 1000-fold increase in absorption compared to aqueous solutions of the hormone, but had no effe ... | 1991 | 1869695 |
| effects of organophosphorus insecticides: effects of trichlorfon and dichlorvos on the immune response of carp (cyprinus carpio). iii. in vitro effects on lymphocyte proliferation and phagocytosis and in vivo effects on humoral response. | cells isolated from the pronephros of carp were incubated in vitro with the organophosphorus insecticide trichlorfon or dichlorvos, each of which is used in aquaculture to eliminate fish ectoparasites. dose-dependent suppressive effects were observed in assays for lymphocyte proliferation and myeloid cell respiratory burst activities. dichlorvos given by bath in vivo did not affect antibody production against yersinia ruckeri even if the spleen and kidney were obviously contaminated according to ... | 1991 | 1914997 |
| [contamination of fish tissue cell cultures by mycoplasma]. | the inoculated and primary cell cultures of fish (carp, salmon, and sturgeon) have been studied. acholeplasma typed as a. laidlawii in terms of its biochemical properties shown in inhibited metabolism has been isolated from 19 samples. the authors consider the source of acholeplasma-induced contamination of the cell cultures under study. | 1991 | 1927041 |
| goussia carpelli (apicomplexa, eimeriorina) from cyprinid fish: field observations and infection experiments. | intestinal tissue samples of 9 cyprinid and gobiid fish species from several hatcheries and ponds in south bohemia were found to harbour goussia carpelli-like coccidian oocysts. furthermore, laboratory transmission of goussia carpelli, obtained from common carp, was attempted to 9 cyprinid fish species, both laboratory reared and obtained from pond environments. infection experiments, carried out by fecal contamination (direct transmission) and by using tubificid oligochaetes as paratenic hosts ... | 1991 | 1928799 |
| insulin-like growth factor i-like immunoreactivity in serum and tissues of the tilapia, oreochromis mossambicus. | tilapia serum was acidified with 0.5 m hcl and then chromatographed on an octadecasily-silica column. after washing with 4% acetic acid, the column was eluted with methanol. the eluate was evaporated to dryness. the sample cross-reacted in a human insulin-like growth factor i (igf-i) radioimmunoassay, suggesting immunochemical similarity to human igf-i. igf-i-like immunoreactivity was present at high levels in tilapia liver. other tissues containing igf-i-like immunoreactivity included the gonad ... | 1991 | 1930252 |
| on the dissemination of two far east monogenean parasites (dactylogyrus achmerovi gussev, 1955 and gyrodactylus kherulensis ergens, 1974, of carp (cyprinus carpio l.)) in czechoslovakia. | 1991 | 1937271 | |
| early development of khawia sinensis hsü, 1935 (cestoda: caryophyllidea), a carp parasite. | the development of the tapeworm khawia sinensis parasitizing carp (cyprinus carpio l.) has been studied from the release of cestode eggs into water to the formation of infective procercoids in the intermediate host under laboratory conditions. the rate of embryonal development is greatly influenced by the water temperature. while at 5 degrees c the development of oncospheres was not recorded, at 10-12 degrees c, 15 degrees c, 20-22 degrees c, and 23-24 degrees c oncospheres were fully formed aft ... | 1991 | 1937272 |
| injection of rna from carp retina induces the formation of a membrane potassium channel in xenopus oocytes. | total rna was purified from freshly isolated retinas of adult carp and injected into oocytes of xenopus laevis (stages 5-6). two to six days after injection, depolarizing voltage-clamp steps evoked a slowly activated outward current as large as 3 microa. this current inactivated slowly with a single time constant (tau = 3.1 +/- 0.24 s.e.m., for vm = +30 mv). the current was inhibited by tetraethylammonium (3.8 mm for half-maximal inhibition). in the presence of co2+ (1 mm) or barium methanesulfo ... | 1991 | 1709045 |
| molecular location of a species-specific epitope on the hamster scrapie agent protein. | scrapie is a transmissible neurodegenerative disease of sheep and goats. an abnormal host protein, sp33-37, is the major protein component of the scrapie agent and the only known disease- or agent-specific macromolecule. two monoclonal antibodies (mabs), 4h8 (immunoglobulin g2b [igg2b]) and 6b11 (igg1), produced by immunizing mice with the intact hamster 263k scrapie agent protein, sp33-37ha, were found to have species specificity similar to that reported previously for mab 3f4 (igg2a), which wa ... | 1991 | 1710287 |
| riboflavin carrier protein from carp (c. carpio) eggs: comparison with avian riboflavin carrier protein. | a protein exhibiting immunological cross-reactivity with the chicken egg-white riboflavin carrier protein was detected by radioimmunoassay in the eggs and serum of the fresh water fish cyprinus carpio and subsequently purified to homogeneity by use of affinity chromatography. fish riboflavin carrier protein resembled chicken riboflavin carrier protein with respect to most of its physicochemical characteristics. the major epitopes of chicken riboflavin carrier protein were shown to be conserved i ... | 1991 | 1713763 |
| an enzyme-linked immunosorbent assay for myelin basic protein-specific protein methylase i. | a sandwich enzyme-linked immunosorbent assay (elisa) has been developed to determine myelin basic protein (mbp)-specific protein methylase i. rabbit immunoglobulin anti-bovine mbp-specific protein methylase i, purified by sepharose-a affinity chromatography, was utilized as the primary antibodies, while the same antibodies which had been conjugated to peroxidase were employed as the indicator antibodies. this assay method was about 280 times more sensitive than the conventional trichloracetic ac ... | 1991 | 1715496 |
| plasmodium falciparum reinfection in children from a holoendemic area in relation to seroreactivities against oligopeptides from different malaria antigens. | the rate and densities of plasmodium falciparum reinfections were investigated in children five to 14 years old from one village in tanzania with a high transmission rate. initial parasitemias were eradicated by a curative treatment with quinine, a drug with a short elimination half-life, to minimize the effects of residual drug on reinfection. the seroreactivities to seven oligopeptides, representing t and b cell epitopes from the ring erythrocyte surface antigen (pf155/resa), the clustered arg ... | 1991 | 1718179 |
| glial fibrillary acidic protein in the fish optic nerve. | the intermediate filament glial fibrillary acidic protein (gfap) is the predominant cytoskeletal protein of mature glial cells in the mammalian nervous system. the nervous systems of lower vertebrates, such as fish, have been examined for the presence of gfap and several investigators have shown that goldfish (carassius auratus) brain contains gfap-positive astrocytes. the same studies have demonstrated that, in contrast to the brain, the optic nerve of goldfish did not show any gfap immunoreact ... | 1991 | 1718861 |
| epitopes of the plasmodium falciparum clustered-asparagine-rich protein (carp) recognized by human t-cells and antibodies. | linear b- and t-cell epitopes have been identified in the plasmodium falciparum clustered-asparagine-rich-protein (carp). twenty-six synthetic peptides, 15-25 amino acids in length, were assayed for their ability to stimulate purified, human t-cells primed to p.falciparum by natural infection to proliferate and/or secrete gamma-interferon (ifn gamma). the plasma of malaria exposed individuals were tested for antibody reactivity with peptides coupled to bovine serum albumin in a semiquantitative ... | 1991 | 1725821 |
| the crystal structure of silver carp insulin at medium resolution. | it is an important way of surveying the structure-function relationship of insulin to study insulins from different species. based on the structure model of an orthorhombic crystal obtained by the molecular replacement method, the crystallographic refinement of a hexamer of silver carp insulin in an asymmetric unit has been carried out with 2.8 a resolution data using the restrained least-squares method. the comparisons of insulin structures have shown that the six silver carp insulin molecules ... | 1991 | 2021422 |
| purification and properties of neutral beta-n-acetylglucosaminidase from carp blood. | a neutral beta-n-acetylglucosaminidase has been purified to homogeneity from carp blood by a seven-step procedure. it was localized in the cytosol of red blood cells. the purified enzyme was specific to beta-n-acetylglucosaminide and inactive to beta-n-acetylgalactosaminide. it was competitively inhibited by free n-acetylglucosamine, but not by free n-acetylgalactosamine. the optimum ph of the enzyme was 6.5, with a stable ph range of 7.0 to 11.0. the enzyme showed greater heat-lability and anod ... | 1991 | 2043684 |
| development of a homologous radioimmunoassay for eel prolactin. | a highly specific and homologous radioimmunoassay (ria) for the measurement of prolactin (prl) in the plasma and the pituitary of the eel was developed using a rabbit antiserum to eel prl. prl was purified from the pituitary of japanese eel (anguilla japonica). pituitary extracts and plasma from the japanese and european eels exhibited displacement curves parallel to the eel prl standard. plasma and pituitary extracts from chum salmon, rainbow trout, japanese charr, tilapia, goldfish, and carp, ... | 1991 | 2055440 |
| evidence for glycosylphosphatidylinositol anchoring of intralumenal alkaline phosphatase of the calf intestine. | 1. considerable amounts of intestinal alkaline phosphatase (ap) were found intralumenally in all animal species investigated, i.e. calf, pig, goat, rat, mouse, guinea pig, hen and carp. the ratios between the total activity of ap found intralumenally and the total intestinal activity vary considerably. calves and pigs show the highest, i.e. 0.77 and 0.44, respectively, while rodents have much lower ratios. only 20-34% of the intralumenal alkaline phosphatase (iap) of the calf and pig is soluble ... | 1991 | 1649047 |
| corticostatic peptides. | in the last four years corticostatic (anti-acth) peptides have been isolated from human, rabbit, guinea pig and rat tissues. these peptides do not act via the camp cell signalling system but rather via the inhibition of the binding of acth to its receptor most probably through direct competition with the 14-18 sequence of acth for receptor binding. acth has specific high affinity receptors on adrenal cells but rabbit corticostatin i (csi) has high capacity, low affinity receptors which are compe ... | 1991 | 1659879 |
| [the spherical theory of color perception: its verification by the methods of psychophysics and neurophysiology]. | in the paper data of experimental verification of "spherical theory of perception" (e. n. sokolov) are summed on the material of colour perception study. the results of psychophysical experiments with subjects having different forms of colour perception (normal trichromats, colour anomalies) and neurophysiological experiments on animals (carp) are considered within single psychophysiological system--spherical model of colour discrimination. neuron-like elements of the model reflect the activity ... | 1991 | 1660642 |
| histochemical and immunocytochemical identification of the pituitary cell types in three sciaenid fishes: atlantic croaker (micropogonias undulatus), spotted seatrout (cynoscion nebulosus), and red drum (sciaenops ocellatus). | the adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. in general greater specificity for the different cell types was observed with antisera to the piscine pituitary hormones than with antisera raised against the human hormones. the distribution of the cell types did not differ significantly among the three species, a ... | 1991 | 1667000 |
| regenerative sodium and calcium currents in goldfish retinal ganglion cell somata. | whole-cell patch-clamp recordings were made from isolated goldfish retinal ganglion cell somata. sodium- and calcium-dependent action potentials--blocked with tetrodotoxin (ttx) and co2+ ions, respectively--were evoked under current-clamp. depolarization of these somata under voltage clamp activated distinguishable na+ and ca2+ conductances: the former was permeable to na+, blocked by ttx, impermeable to n-methyl-d-glucamine and tris(hydroxymethyl)aminomethane, and began to activate around -45 m ... | 1991 | 1668863 |
| presence of prolactin receptors in eel liver and carp kidney and growth hormone receptor in eel liver. | 1. 125i-labelled ovine prolactin and bovine growth hormone were used to test for the presence of prolactin and growth hormone receptors in membrane prepared from tissues of the white eel anguilla japonica, the carp ctenopharynogodon idellus and the ricefield eel monopterus albus. 2. high levels of specific 125i-labelled ovine prolactin binding were found in white eel liver membranes and carp kidney membranes. 3. high levels of specific 125i-labelled bovine growth hormone binding were detected in ... | 1991 | 1678335 |
| retinal morphology of cyprinid fishes: a quantitative histological study of ontogenetic changes and interspecific variation. | morphological patterns of the retina, cone size and density, rod density, rod-cone ratio, ganglion cell density, convergence of receptor cells, resolving power (rp) and regionalization were examined throughout life history in roach and in adults of asp, bream, common carp, roach and sabre carp. the retina of hatchlings is packed with small cones. during larval and juvenile growth the retina stretches, cones increase in diameter and rods are present in increasing numbers. photopic and scotopic se ... | 1991 | 1843750 |
| gene transfer in common carp (cyprinus carpio l.) by microinjection into the germinal disc. | we report here on the microinjection of the human growth hormone gene (hgh) into the germinal disc of common carp one-cell embryos. for microinjection, a linear 4.1 kb ecori-dna fragment containing the mouse metallothionein-i promoter (mmt-i), fused to a structural gene coding for the hgh, was used. of the 14 injected embryos, 9 developed to an age of 60 d. of these nine fish, two were found to be transgenic and had integrated two to four copies per cell of the hgh construct. | 1991 | 16726931 |
| immunoreactive endothelin in plasma of nonmammalian vertebrates. | immunoreactive endothelin (ir-et) was estimated in plasma of nonmammalian vertebrates, using radioimmunoassay (ria) for endothelin-1 (et-1). blood samples were collected from unanesthetized animals. plasma et was determined by ria after extraction. plasma levels of ir-et were 0.7 +/- 0.2 pg/ml (n = 6) in the hagfish eptatretus burgeri, 4.3 +/- 0.9 pg/ml (n = 5) in the banded dogfish triakis scyllia, 3.6 +/- 0.8 pg/ml (n = 3) in the common japanese conger conger myriaster, 9.6 +/- 1.4 pg/ml (n = ... | 1991 | 1725396 |
| investigations into the effects of concentration and duration of exposure to formalin and malachite green on the survival of the larvae and juveniles of the common carp cyprinus carpio l. and the sharptooth catfish clarias gariepinus (burchell). | prophylactic dip treatments using formalin and malachite green were applied to 4-day old larvae and 12- and 20-day old juveniles of the european common carp, cyprinus carpio and the african sharptooth catfish, clarias gariepinus. treatments consisted of 100 mg/l malachite green for exposure periods of 10, 30 or 90 s and 200 mg/l formalin, administered for 30, 60 or 90 min. larvae and juveniles of c. gariepinus could be treated with 100 mg/l malachite green for 10 s, or with 200 mg/l formalin for ... | 1991 | 1780124 |
| treatment of cyprinus carpio l. and clarias gariepinus (burchell) embryos with formalin and malachite green: effect of concentration and length of treatment on their survival. | malachite green and formalin were investigated as prophylactic dip treatments for developing embryos of cyprinus carpio and clarias gariepinus. based on the findings, recommendations are made on the respective concentrations to be used and exposure periods to be followed for both formalin and malachite green for both fish species. | 1991 | 1780123 |
| the adenosine receptor blocker aminophylline increases anoxic ethanol excretion in crucian carp. | by depressing energy consumption, anoxia-tolerant animals are thought to compensate for a reduced ability to produce energy during anoxia. adenosine is an inhibitory neuromodulator in vertebrates and, hence, has the potential ability to depress energy consumption. ethanol is the main metabolic end product in anoxic carassius, and the present study shows that the rate of ethanol excretion in anoxic crucian carp (carassius carassius l.) can be increased threefold by treatment with the adenosine re ... | 1991 | 1928423 |
| biocytin: intracellular staining, dye-coupling and immunocytochemistry in carp retina. | correlation of electrophysiological and morphological, including ultrastructural, characteristics of neurones is important for understanding the functional organization of neuronal systems. further correlation with neurotransmitter content is essential for determining the neurochemical(s) used by a given neurone for propagating its signal. the two main neuronal markers presently available (lucifer yellow and horseradish peroxidase) are not satisfactory for correlating all three aspects. we have ... | 1991 | 1724386 |
| population study of horizontal cells in live carp retinas using microinjections of horseradish peroxidase. | in order to randomly sample horizontal cells (hcs) of carp retina, horseradish peroxidase (hrp) was delivered into axon terminals by means of vitreally inserted micropipettes in live fish. survival time was extended to several hours to permit label diffusion into hc somata. a function measuring the relative complexity of cell contours was defined in order to help classify hcs according to their morphological types. the histogram of measurement from 313 hcs showed three modes fitting normal distr ... | 1991 | 1891806 |
| effects of 17 beta-estradiol and carp gonadotropin on vitellogenesis in normal and hypophysectomized european silver female eel (anguilla anguilla l.) employing a homologous radioimmunoassay for vitellogenin. | the european silver eels are at the early stages of vitellogenesis before the marine reproductive migration. vitellogenesis was induced by 17 beta-estradiol (e2) alone and by a purified carp gonadotropin (cgth). we studied and compared their effects on plasma vitellogenin (vg) levels and ovarian yolk contents in female normal (n) and hypophysectomized (h) eels for both treatments. to this purpose an homologous radioimmunoassay (ria) was established. eel vg was purified to homogeneity on 0.1% sds ... | 1991 | 1783271 |
| comparative study of acetylcholine synthesis in organs of freshwater teleosts. | the activity of acetylcholine (ach) synthesizing enzyme, choline acetyltransferase (chat), and the content of ach were determined in the brain, heart, red trunk muscle and midgut of 12 freshwater teleost species belonging to eight families: carp (cyprinus carpio), tench (tinca tinca), silver carp (hypophthalmichtys molitrix), bighead (hypophthalmichtys nobilis), wels (silurus glanis), cat fish (ictalurus nebulosus), eel (anguilla anguilla), rainbow trout (oncorhynchus mykiss), pike (esox lucius) ... | 1991 | 24214667 |
| in vitro study on the impact of fish sera on the survival and fine structure of the eel-pathogenic acanthocephalan paratenuisentis ambiguus. | the effects of fish sera on the growth and fine structure of infective larvae of the eel-pathogenic acanthocephalan paratenuisentis ambiguus (eoacanthocephala: tenuisentidae) were studied under in vitro conditions using sera from the final host anguilla anguilla and from two accidental fish hosts as well as fetal calf serum. as controls larvae were also kept in medium in the absence of serum and in experimentally infected eels. sera from the accidental fish hosts carp and rainbow trout exerted t ... | 1991 | 1805215 |
| inhibition of mg2+ and na(+)-k+ atpases in selected tissues of fish, cyprinus carpio under fenvalerate toxicity. | the changes in the magnesium adenosine triphosphatase (mg2+ atpase) and sodium-potassium adenosine triphosphatase (na(+)-k+ atpase) in gill, brain, liver and muscle tissues of freshwater fish, cyprinus carpio at 6, 12, 24 and 48 hr exposure periods were studied after subjecting to sublethal concentration (10 micrograms/lit) of fenvalerate. mg2+ atpase and na(+)-k+ atpase activities were inhibited in all the tissues of fenvalerate exposed fish. the per cent inhibition increased with increase in t ... | 1991 | 1651727 |
| cloning and characterization of the carp prolactin gene. | a carp genomic dna clone containing the carp prolactin (prl) gene was isolated with carp prl cdna as a probe. the organization of the carp prl gene was determined by restriction nuclease mapping and nucleotide sequencing. the prl gene comprises approx. 2.8 kilobasepairs (kb) of dna including the 5'-flanking region, five exons, four introns and the 3'-flanking region. analysis of the 5'-flanking region reveals (1) the sequence tatataat at positions -38 to -31 upstream from the cap site which was ... | 1991 | 2001405 |
| infection of the heart in carp caused by myxobolus dogieli (myxosporea, myxobolidae). | myxobolus dogieli infecting the heart muscles of carp was noted in a cage fishfarm situated in the upper volga river. prevalence of infection of two summer old fishes in different cages was 10-20%. some of the fishes perished. one summer old carps had a lower infection. the disease takes place when the thermal efflusences of power stations are used. | 1991 | 2039093 |
| myofilament overlap in swimming carp. ii. sarcomere length changes during swimming. | this study was performed to determine myofilament overlap during swimming in carp. by using frozen sections, we found that sarcomere lengths of the red and white muscle could be related to the curvature of the backbone. sarcomere length (sl) during swimming was calculated from an analysis of backbone curvature in high-speed motion pictures. because carp have the same myofilament lengths as frogs, we related force generation to sl using the frog sl-tension curve. at slow swimming speeds, the red ... | 1991 | 1996612 |
| myofilament overlap in swimming carp. i. myofilament lengths of red and white muscle. | to assess myofilament overlap during locomotion, we estimated the length of myosin and actin filaments in axial red and white muscle of carp. myosin filament lengths were 1.52 +/- 0.009 and 1.50 +/- 0.037 micron (means +/- sd) in the red and white muscle, respectively, as measured from thin sections. after correction for shrinkage (using the troponin-based 385-a axial periodicity), thin filaments were 0.96 +/- 0.009 and 0.97 +/- 0.023 micron in the red and white muscles, respectively. filaments ... | 1991 | 1996611 |
| metabolism of benzo[a]pyrene and (-)-trans-benzo[a]pyrene-7,8-dihydrodiol by freshly isolated hepatocytes from mirror carp. | the metabolism of benzo[a]pyrene (b[a]p) and (-)-trans-benzo[a]pyrene-7,8-dihydrodiol [(-)-b[a]p-7,8-diol], a major putative proximate carcinogenic metabolite of b[a]p, was compared in freshly isolated hepatocytes from mirror carp, a strain of common carp (cyprinus carpio, l.). hepatocytes incubated with 40 microm [3h]b[a]p produced 1.22 nmol equivalents of b[a]p metabolites/mg dry wt of cells/h. conjugated derivatives represented approximately 65% of all b[a]p metabolites and included glucuroni ... | 1991 | 1995182 |