Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cloning and characterization of two full-length cdnas, taga1 and taga2, encoding g-protein alpha subunits expressed differentially in wheat genome. | in the present study, we identified and characterized two cdnas, named taga1 and taga2, encoding alpha subunits of heterotrimeric g proteins synthesized from one-week-old seedling mrnas of common wheat cv. s615 using race pcr and rt-pcr methods. the clone taga1 contained an open reading frame that encoded a protein consisting of 383 amino acid residues with a molecular mass of 51.3 kda, whereas the clone taga2 contained an open reading frame encoding 390 amino acids with a molecular mass of 52.5 ... | 2003 | 12773813 |
| environmentally-induced changes in protein composition in developing grains of wheat are related to changes in total protein content. | nitrogen (n) nutrition, post-anthesis temperature and drought-induced changes in the kinetics of accumulation of dry mass, total grain n and protein fractions (albumins-globulins, amphiphils, gliadins, and glutenins) contents were examined for winter wheat (triticum aestivum l.). crops were grown in controlled environment tunnels in 1994 and 1998. in 1994, five post-anthesis temperatures averaging from 15-25 degrees c were applied during grain-filling. in 1998 two post-anthesis temperatures aver ... | 2003 | 12773520 |
| salicylic acid changes the properties of extracellular peroxidase activity secreted from wounded wheat (triticum aestivum l.) roots. | wheat ( triticum aestivum l.) roots released proteins showing peroxidase activity in the apoplastic solution in response to wound stress. preincubation of excised roots with 1 mm salicylic acid at ph 7.0 enhanced the guaiacol peroxidase activity of the extracellular solution (so-called extracellular peroxidase). the soluble enzymes were partially purified by precipitation with ammonium sulfate followed by size exclusion and ion exchange chromatography. despite an increase in the total activity o ... | 2003 | 12768343 |
| evaluation of cytological effects of zn2+ in relation to germination and root growth of nigella sativa l. and triticum aestivum l. | the effects of different treatments with zinc sulfate (zn(2+)) on the cytology and growth of nigella sativa and triticum aestivum were investigated. five concentrations of zinc sulfate ranging from 5 to 25mg/l were applied for 6, 12, 18, and 24h. the treatments reduced the germination percentages of n. sativa seeds and t. aestivum grains and inhibited the root growth of both plants. concentrations higher than 25mg/l of zn(2+) applied for 24h were toxic for both plants. the non-lethal concentrati ... | 2003 | 12742505 |
| [effects of genotype and environment on wheat grain quality and protein components]. | the variations of grain qualities and protein components of 40 wheat (triticum aestivum) varieties were studied at three sites in 1998 and 1999. the results showed that there were significant differences among the genotypes in all the quality characters and protein components. the 40 wheat varieties were divided into 6 groups by comprehensive characters, which mainly included semi-hard and soft wheat in the experimental environment. the effects of environment were significant on grain test weigh ... | 2003 | 14986358 |
| cloning and expression of pinb gene from triticum monococum seeds. | puroindolines (pin) are low molecular weight, cysteine-rich, endosperm-specific, basic proteins with a unique tryptophan-rich domain found in wheat (triticum aestivum l.) and barley (hordeum vulgare l.) as well as other members of triticaceae. pins appear to be involved in both flour softness as well as resistance against fungal diseases. these proteins are known to be the major components of 'friabilin' associated with the surface of water washed starch grains and possess lipid binding properti ... | 2003 | 15151287 |
| antisense snf1-related (snrk1) protein kinase gene represses transient activity of an alpha-amylase (alpha-amy2) gene promoter in cultured wheat embryos. | a dna fragment corresponding to part of an snf1 (sucrose non-fermenting-1)-related protein kinase (snrk1) transcript was amplified by a polymerase chain reaction (pcr) from a wheat (triticum aestivum) endosperm cdna library. it was used to construct a chimaeric gene, puassnrkn, comprising a ubiquitin promoter, the snrk1 pcr product in the antisense orientation and the nopaline synthase (nos) gene terminator. this construct was used in transient gene expression experiments in cultured wheat embry ... | 2003 | 12554717 |
| [rapd marker analysis of continuous divergent selection in cross-bred population of wheat (triticum aestivum l.)]. | a base population was established through multi-parent random crossing by using taigu dominant male-sterile wheat, and then five cycles of 2-way selection for four quantitative characters were conducted. the dynamic changes of genetic structures in the open-pollinated wheat population were examined by rapd technique. seven primers in rapd analysis amplified 116 sites. the results of gene frequencies and phenotypic bands showed abundant genetic variations existed in the population. the percentage ... | 2003 | 15639875 |
| [chromosome analysis of chongqing thermo-photo sensitive male sterile wheat]. | seed gliadin acid-polyacrylamide-gel-electrophoresis (apage) and chromosome c-banding techniques were used to identify chromosome constitution of chongqing thermo-photo sensitive male sterile wheat. results showed that 1bs of the male sterile lines was substituted by 1rs of rye. they were 1b/1r wheat with triticum aestivum cytoplasm. | 2002 | 16118133 |
| [advances in studies of genome-specific repetitive dna sequences in wheat and related species]. | in this paper we review recent advances in studies of several aspects of genome specific repetitive dna sequences in wheat and related species. the available results demonstrate that genome specific repetitive dna sequences are important components of genome specificity in wheat and related species. research on genome specific repetitive dna sequences is essential to the elucidation of genome function. the application of genome specific repetitive dna sequences will aid molecular cytogenetic stu ... | 2002 | 16135457 |
| improving intrinsic water-use efficiency and crop yield. | greater yield per unit rainfall is one of the most important challenges in dryland agriculture. improving intrinsic water-use efficiency (w(t)), the ratio of co(2) assimilation rate to transpiration rate at the stomata, may be one means of achieving this goal. carbon isotope discrimination (delta(13)c) is recognized as a reliable surrogate for w(t) and there have now been numerous studies which have examined the relationship between crop yield and w(t) (measured as delta(13)c). these studies hav ... | 2002 | 11756262 |
| biplot analysis of diallel data. | diallel crosses have been used in genetic research to determine the inheritance of important traits among a set of genotypes and to identify superior parents for hybrid or cultivar development. conventional diallel analysis is limited to partitioning the total variation of the data into general combining ability (gca) of each genotype and specific combining ability (sca) of each cross. in this paper we formulate a biplot approach for graphical diallel analysis. the biplot is constructed by the f ... | 2002 | 11756249 |
| changes in mass and energy transfer between the canopy and the atmosphere: model development and testing with a free-air co2 enrichment (face) experiment. | the rationale for this study is found in the probable higher temperatures and changes in rainfall patterns that are expected in the future as a result of increasing levels of co2 in the atmosphere. in particular, higher air temperatures may cause an increase in evapotranspiration demand while a reduction in rainfall could increase the severity and duration of drought in arid and semi-arid regions. representation of the water transfer scheme includes water uptake by roots and the interaction betw ... | 2002 | 11931099 |
| activation of polyphenol oxidase in extracts of bran from several wheat (triticum aestivum) cultivars using organic solvents, detergents, and chaotropes. | polyphenol oxidase (ppo), known to induce browning in wheat-based products, has been shown to be activatable in wheat (triticum aestivum) bran extracts by chemical compounds. the activity in the extracts could be increased to varying degrees with acetone, methanol, ethanol, 2-propanol, and n-butanol as additives in the extraction buffer. the most potent alcoholic activator was n-butanol (about a 3-fold increase), followed by 2-propanol and ethanol, whereas methanol had the least effect. ionic de ... | 2002 | 11929305 |
| temperature acclimation of photosynthesis and related changes in photosystem ii electron transport in winter wheat. | winter wheat (triticum aestivum l. cv norin no. 61) was grown at 25 degrees c until the third leaves reached about 10 cm in length and then at 15 degrees c, 25 degrees c, or 35 degrees c until full development of the third leaves (about 1 week at 25 degrees c, but 2-3 weeks at 15 degrees c or 35 degrees c). in the leaves developed at 15 degrees c, 25 degrees c, and 35 degrees c, the optimum temperature for co(2)-saturated photosynthesis was 15 degrees c to 20 degrees c, 25 degrees c to 30 degree ... | 2002 | 11891263 |
| an application of linear regression technique for predicting bulgur yield and quality of wheat cultivars. | grains of 26 turkish wheat cultivars and advanced breeding lines were used in this study. simple correlations between a number of quality parameters to predict bulgur yield and bulgur cooking quality were determined. highly significant correlations between bulgur yield and each of the thousand-kernel weight and the sum of the grain over 2.8 + 2.5 mm sieves were obtained for both durum and bread wheat samples (p < 0.01). the regression equations showed that the models involving two variables (the ... | 2002 | 11890049 |
| ecological toxicity of reactive x-3b red dye and cadmium acting on wheat (triticum aestivum). | ecological toxicity of reactive x-3b red dye and cadmium in both their single form and their combined form on wheat was studied using the experimental method of seed and root exposure. the single-factor exposure indicated that the inhibitory rate of wheat root elongation was significantly increased with the increase in the concentration of the dye in the cultural solution, although seed germination of wheat was not sensitive to the dye. the toxicity of cadmium was greatly higher than that of the ... | 2002 | 11887311 |
| synergistic action of ultraviolet-b radiation and cadmium on the growth of wheat seedlings. | the increase in ground level uv-b radiation as a result of stratospheric ozone depletion may have major deleterious effects on crop photosynthesis and productivity. plants are exposed to uv-b and other xenobiotics simultaneously in today's industrialized world. the present studies were undertaken to see the effect of dual stress of uv-b and cd2+ exposure on the growth of wheat (triticum aestivum l.) seedlings. the plants grown in 0.25, 0.5, 1.0, 2.5, and 5.0 ppm cd2+-supplemented medium were exp ... | 2002 | 11886182 |
| the sugary-type isoamylase in wheat: tissue distribution and subcellular localisation. | to gain an increased understanding of the role of isoamylase (ec 3.2.1.68) in amylopectin synthesis, we studied the tissue-specific distribution and subcellular localisation of this enzyme in wheat (triticum aestivum l.). a cdna for wheat isoamylase was isolated from an endosperm-specific library and the missing 5' end was amplified by anchored polymerase chain reaction. isoamylase transcripts were detected in reproductive and vegetative tissues, with the highest levels occurring in developing k ... | 2002 | 11882952 |
| occurrence of zearalenone-4-beta-d-glucopyranoside in wheat. | an lc-ms method was developed for the analysis of zearalenone-4-beta-d-glucopyranoside and zearalenone in wheat (triticum aestivum). the limit of determination for zearalenone-4-beta-d-glucopyranoside and zearalenone was 10 microg/kg. the recovery rates were calculated to be 69% and 89% at a concentration of 100 microg/kg for zearalenone-4-beta-d-glucopyranoside and zearalenone, respectively. twenty-four bavarian wheat samples from a 1999 harvest were analyzed. zearalenone was present in 22 of 2 ... | 2002 | 11879067 |
| free radical scavenging properties of wheat extracts. | three hard winter wheat varieties (akron, trego, and platte) were examined and compared for their free radical scavenging properties and total phenolic contents (tpc). free radical scavenging properties of wheat grain extracts were evaluated by spectrophotometric and electron spin resonance (esr) spectrometry methods against stable 2,2-diphenyl-1-picryhydrazyl radical (dpph*) and radical cation abts*+ (2,2'-azino-di[3-ethylbenzthiazoline sulfonate]). the results showed that the three wheat extra ... | 2002 | 11879046 |
| cadmium and zinc interactions and their transfer in soil-crop system under actual field conditions. | the transfer of cd and zn from calcareous soils nearby a non-ferrous mining and smelting bases to the spring wheat (triticum aestivum l.) and corn (zea mays l.) tissues and the interactions between the two metals concerned were investigated under actual field conditions. samples of soils and entire crops were randomly collected during harvest time in 1998 in the baiyin region. the soil metal contents showed that the furrows had been polluted (mean values: 3.16 mg kg(-1) for cd; 146.78 mg kg(-1) ... | 2002 | 11874041 |
| a gene encoding multidrug resistance (mdr)-like protein is induced by aluminum and inhibitors of calcium flux in wheat. | a cdna clone exclusively induced by aluminum (al) was isolated from root apices of wheat (triticum aestivum l.) by the differential display method. the predicted amino acid sequence exhibited homology to the multidrug resistance (mdr) proteins that is known as a member of the atp-binding cassette (abc) protein superfamily. thus this gene was named tamdr1 (triticum aestivum mdr). tamdr1 was induced as a function of al concentration in the range from 5 to 50 microm, which is in the range of al con ... | 2002 | 11867697 |
| in-situ comparative mapping (iscm) of glu-1 loci in triticum and hordeum. | the ability to detect small low- or single-copy dna sequences by fluorescence in-situ hybridization (fish) is an important step towards physical mapping of plant genomes. in this study, the fish technique was used to physically map the glu-1 loci controlling high-molecular weight (hmw) glutenin in common wheat (triticum aestivum cv. 'chinese spring') and tritordeum (an amphiploid between t. turgidum cv. durum and hordeum chilense). the probe used was the single-copy glu-d1-1d gene coding the 1dx ... | 2002 | 11863070 |
| adp-glucose pyrophosphorylase from wheat endosperm. purification and characterization of an enzyme with novel regulatory properties. | adp-glucose pyrophosphorylase (agpase; ec 2.7.7.27) was purified and characterized from two wheat (triticum aestivum l.) tissues: leaf and endosperm. the leaf enzyme, purified over 1,300-fold, was found to be a heterotetramer composed of subunits of 51 and 54 kda and possessing regulatory properties typical of agpases from photosynthetic tissues, being mainly regulated by 3-phosphoglycerate (activator; a0.5=0.01 mm) and orthophosphate (inhibitor; i0.5=0.2 mm). conversely, the enzyme from wheat e ... | 2002 | 11855648 |
| [study of the backcross progeny of barley-wheat hybrids by the rapd and rampo methods]. | the genomes of alloplasmic wheat lines were analyzed by pcr-based methods: random amplified polymorphic dna (rapd) and random amplified microsatellite polymorphism (rampo). lines l-16(1) and l-17(2) were obtained by three backcrosses and line l-79(10), by four backcrosses of the barely-wheat hybrid hordeum vulgare (2n = 14) (variety nepolegayuschii) x triticum aestivum (2n = 42) (variety saratovskaya 29) with different common wheat varieties. these lines proved to be euploid (2n = 42). the aneup ... | 2002 | 11963561 |
| vernalization-induced changes of the dna methylation pattern in winter wheat. | vernalization is a cold treatment that induces or accelerates flowering and insures that temperate-zone plants will not flower until after winter. there is evidence that vernalization results in dna demethylation that induces flowering. differences in dna methylation can be determined using methylation-sensitive amplified fragment length polymorphisms (aflps). methylation-sensitive aflps utilize restriction enzyme isoschizomers that are differentially sensitive to methylation, producing polymorp ... | 2002 | 11962622 |
| wheat fkbp73 functions in vitro as a molecular chaperone independently of its peptidyl prolyl cis-trans isomerase activity. | peptidyl-prolyl cis-trans isomerases (ppiases) catalyse protein folding by accelerating the slow step of cis-trans isomerisation of peptidyl-prolyl bonds. wheat (triticum aestivum l.) fkbp73 (wfkbp73) is a peptidyl-prolyl cis-trans isomerase belonging to the fk506-binding protein (fkbp) family. it comprises three fkbp12-like domains, tetratricopeptide repeats and a calmodulin-binding domain (cambd). in vitro studies indicated that wfkbp73 possesses ppiase activity, binds calmodulin and forms a h ... | 2002 | 12012248 |
| a new biochemical marker for aluminium tolerance in plants. | al was shown to elicit the induction of several pathogenesis-related genes, suggesting that a common signalling pathway may be involved in the early response to al and pathogens. however, we found no evidence of oxidative burst involving either h2o2 or o2- during the first hours of al exposure distinguishing the early response to al from a common response to pathogen infection. we identified a strong superoxide dismutase insensitive nitro blue tetrazolium (nbt) reduction activity in the root tip ... | 2002 | 12010470 |
| o-glucosyltransferase activities toward phenolic natural products and xenobiotics in wheat and herbicide-resistant and herbicide-susceptible black-grass (alopecurus myosuroides). | herbicide safeners manipulate herbicide selectivity by enhancing the activities of detoxifying enzymes, such as glutathione transferases (gsts) and cytochrome p450 mono-oxygenases (cyps) in cereal crops. as part of a study examining the importance of o-glucosyltransferases (ogts) in pesticide metabolism in hexaploid bread wheat (triticum aestivum l.), seedlings were grown in the presence of dichlormid, a safener used in maize and cloquintocet mexyl, a wheat safener. the efficacy of the treatment ... | 2002 | 11809449 |
| pistillody, homeotic transformation of stamens into pistil-like structures, caused by nuclear-cytoplasm interaction in wheat. | homeotic transformation of stamens into pistil-like structures (pistillody) has been observed in a cytoplasmic substitution (alloplasmic) line of wheat (triticum aestivum l.) cv. norin 26, which has the cytoplasm of a wild relative species, aegilops crassa l. on the other hand, an alloplasmic line of wheat cv. chinese spring (cs) with ae. crassa cytoplasm has normal flowers. this is due to the presence in the cs nucleus of a fertility-restoring gene, rfd1. deletion mapping analysis revealed that ... | 2002 | 11851918 |
| nitrogen assimilation and growth of wheat under elevated carbon dioxide. | simultaneous measurements of co(2) and o(2) fluxes from wheat (triticum aestivum) shoots indicated that short-term exposures to elevated co(2) concentrations diverted photosynthetic reductant from no(3)(-) or no(2)(-) reduction to co(2) fixation. with longer exposures to elevated co(2), wheat leaves showed a diminished capacity for no(3)(-) photoassimilation at any co(2) concentration. moreover, high bicarbonate levels impeded no(2)(-) translocation into chloroplasts isolated from wheat or pea l ... | 2002 | 11818528 |
| production of near-isogenic lines and marked monosomic lines in common wheat (triticum aestivum) cv. chinese spring. | sixteen near-isogenic lines (nils) carrying a marker gene were produced by the recurrent backcrossing method in the genetic background of common wheat (triticum aestivum) cv. chinese spring (cs). three genes from alien species showed segregation distortion. in nils carrying a marker gene of rye (secale cereale) or aegilops caudata, the alien chromosome segments were detected by fluorescence in situ hybridization (fish). the nils were grown with replications and the effect of marker genes on plan ... | 2002 | 11447241 |
| production of wheat doubled haploids by pollination with job's tears (coix lachryma-jobi. l.). | wheat (triticum aestivum l.) haploids were produced by crossing with job's tears (coix lachryma-jobi l.) as the pollen parent. pollination was followed by 2,4-d treatment, detached tiller culture, and embryo culture, as described for maize pollination. the frequency of embryo formation was similar to that obtained by crossing wheat with maize pollen. job's tears is a perennial plant which forms several stalks and its pollen can be collected throughout the year when the plant is maintained in a c ... | 2002 | 11336235 |
| [effect of modification of cell calcium status on lectin activity]. | effects of oryzalin (10 microm), an inhibitor of microtubule polymerization, on the activity of soluble and cell wall lectins were studied in 7 day-old seedlings of unhardened (23 degrees c) and cold acclimated (7 days at 2-3 degrees c) winter wheat (triticum aestivum l.). seedlings were grown in the presence of 25 microm and 1 mm ca2+, 500 microm verapamil, 250 microm chlorpromazine or without modifiers of calcium status in the medium. inhibitor of the microtubule polymerization inhibitor, like ... | 2002 | 14696259 |
| inheritance of dense spike in diploid wheat and aegilops squarrosa. | the individuals of diploid wheat triticum boeoticum, t. monococcum and t. sinskajae and goatgrass aegilops squarrosa were picked out with screening the dense spike characteristics. the dense-spike accessions were discovered in diploid wheat (t. sinskajae) and ae. squarrosa. inheritance of the dense spike was studied. the trait was found to be controlled by a recessive gene in t. sinskajae and by an incomplete dominant gene in ae. squarrosa. the dosage effect of dominant gene c was detected in in ... | 2002 | 12627833 |
| [genetic mapping of the intergenomic translocated "taigu" genic sterility gene (ms2)]. | the "taigu" genic sterility gene ms2 located on the short arm of the 4d chromosome of common wheat (aabbdd) originally incorporated into hexoploid triticale (aabbrr) and durum wheat (aabb) through intergenomic translocation in distant hybridization was introduced back into the genomes of common wheat. the dominant male sterility was expressed normally in the new "taigu" genic sterile wheat carrying the intergenomically translocated ms2, and the female fertility mechanism in its male sterile plan ... | 2002 | 12693100 |
| use of sampl for a study of dna polymorphism, genetic diversity and possible gene tagging in bread wheat. | selective amplification of microsatellite polymorphic loci (sampl) technology was used in bread wheat for the first time for a study of genetic diversity, genotype identification and gene tagging. the diversity studies involved 55 wheat genotypes and two sampl primer pairs (sampl-6 and sampl-7, each with a m-cag primer), which together gave 43 polymorphic bands out of a total of 87 sampl bands. the average polymorphic information content (pic) of sampl primers was 0.221 and that of sampl markers ... | 2002 | 12582720 |
| characterisation and chromosomal localisation of c-type low-molecular-weight glutenin subunits in the bread wheat cultivar chinese spring. | low-molecular-weight glutenin subunits are classically divided into the b, c and d groups. most attention has been paid to the characterisation of the b and d groups, whereas c subunits, although represented by a large number of protein components, have not been thoroughly characterised, mainly because they tend to separate with the gliadins in many fractionation procedures. here we describe a procedure for obtaining a fraction strongly enriched in c subunits that has allowed us to determine the ... | 2002 | 12582715 |
| is the mnr locus of triticeae species the same as the ndh and dia loci? | the menadione reductase (mnr), the nicotinamide adenine dinucleotide dehydrogenase (ndh) and diaphorase (dia) isozymes were studied in the allohexaploid triticum aestivum cv "chinese spring" and in five diploid triticeae species. the mnr1, ndh3and dia1 loci were located on the chromosome arms 3al, 3bl and 3dl of t. aestivum, respectively. these loci were also located on the 3h chromosome of hordeum vulgare cv "betzes", the 3l chromosome of aegilops longissima and the 6rl chromosome arm of secale ... | 2002 | 12582652 |
| the molecular genetic characterization of the 'bobwhite' bread wheat family using aflps and the effect of the t1bl.1rs translocation. | bobwhite is a generic name that refers to all sister lines derived from the cross cm 33203 with the pedigree aurora//kalyan/bluebird/3/woodpecker made by the cimmyt bread wheat program in the early 1970s. individual sister lines can be distinguished by their unique selection history. one of the parents, aurora, contains the t1bl.1rs translocation from rye, and approximately 85% of the sister lines have inherited the translocation. the sister lines demonstrate great variability for agronomic trai ... | 2002 | 12582648 |
| molecular characterization of wheat polyphenol oxidase (ppo). | it is well-established that the enzyme polyphenol oxidase (ppo) is involved in undesirable browning of noodles, chapattis, middle east flat breads and steamed breads. methods for measuring ppo activity have been developed, and the variation of ppo activity among wheat ( triticum aestivum l.) cultivars has been well documented. however, there is no report on the identification and characterization of a wheat ppo gene. pcr performed on wheat genomic dna with oligonucleotide primers designed from c ... | 2002 | 12582641 |
| the effect of a deficiency and a deletion on recombination in chromosome 1bl in wheat. | to test two models of chiasma allocation and the distribution of crossing-over in chromosomes, genetic mapping was performed in normal, deletion and deficiency chromosome arms 1bl of wheat, triticum aestivum l. shortening of the chromosome arm, either by a deletion of the proximal half of the arm or by a deficiency of the terminal quarter of the arm's length, significantly reduced the frequency of multiple crossovers but did not affect the distribution of the distal, presumably the first, crosso ... | 2002 | 12582631 |
| [hydrous and photosynthetic adaptations of common and durum wheat to saline stress]. | seven varieties of bred wheat and seven varieties of durum wheat were cultivated in three different sites from the area of errachidia (southeastern morocco). these sites differ by the degree of salinity in the irrigation water. results obtained showed that the reduction in leaf area is the principal strategy that makes it possible to attenuate the effects of the reduction in the availability of water under saline stress. bread wheat, which limited the reduction in the leaf area, with the risk to ... | 2002 | 12506723 |
| the voltage-independent cation channel in the plasma membrane of wheat roots is permeable to divalent cations and may be involved in cytosolic ca2+ homeostasis. | a voltage-independent cation (vic) channel has been identified in the plasma membrane of wheat (triticum aestivum) root cells (p.j. white [1999] trends plant sci 4: 245-246). several physiological functions have been proposed for this channel, including roles in cation nutrition, osmotic adjustment, and charge compensation. here, we observe that ca(2+) permeates this vic channel when assayed in artificial, planar lipid bilayers, and, using an energy barrier model to describe cation fluxes, predi ... | 2002 | 12428003 |
| differential effects of cultivated and wild barley 5h chromosomes on heading characters in wheat-barley chromosome addition lines. | wheat (triticum aestivum l.)-barley (hordeum vulgare l.) chromosome addition lines are possible vehicles for transferring barley genes into wheat. the barley 5h chromosome has genetic effects on the heading characters in wheat-barley addition lines: accelerating narrow-sense earliness, decreasing vernalization requirement and/or increasing photoperiodic sensitivity. to elucidate the effects of different 5h chromosomes under an identical wheat genetic background, two wheat-barley addition lines, ... | 2002 | 12471665 |
| sequence tagged microsatellite profiling (stmp): improved isolation of dna sequence flanking target ssrs. | sequence tagged microsatellite profiling (stmp) enables the rapid development of large numbers of co-dominant dna markers, known as sequence tagged microsatellites (stms). each stm is amplified by pcr using a single primer specific to the conserved dna sequence flanking the microsatellite repeat in combination with a universal primer that anchors to the 5'-ends of the microsatellites. it is also possible to convert stms into conventional microsatellite, or simple sequence repeat (ssr), markers t ... | 2002 | 12466561 |
| mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat ( triticum aestivum l.). | a set of 114 recombinant inbred lines of the 'international triticeae mapping initiative' mapping population was grown during the seasons 1997, 1998, 1999 and 2000 under several environments. twenty morphological (glume colour, awn colour, waxiness, leaf erectness, peduncle length), agronomical (ear emergence time, flowering time, grain filling time, ear length, plant height, lodging, grain number, thousand-grain-weight, grain weight per ear, grain protein content, winter hardiness) and disease ... | 2002 | 12582918 |
| an estimation of the minimum number of ssr alleles needed to reveal genetic relationships in wheat varieties. i. information from large-scale planted varieties and cornerstone breeding parents in chinese wheat improvement and production. | the genetic relationships of 43 wheat varieties were analyzed with ssr markers. the materials employed included 14 cornerstone breeding parents used before 1980 and another 29 other large-scale planted varieties currently in use in china. a total of 501 different alleles were amplified, including 166 alleles of the a genome, 174 of the b genome and 161 of the d genome. data obtained were used to estimate genetic similarity using the dice coefficient, and dendrograms were constructed using the up ... | 2002 | 12582878 |
| microsatellite analysis of wheat chromosome 2d allows the reconstruction of chromosomal inheritance in pedigrees of breeding programmes. | the dwarfing gene rht8 and the photoperiodic insensitivity gene ppd-d1 are linked on the short arm of chromosome 2d of bread wheat and play an important role in determining the geographic adaptation of modern wheat varieties. the genes are believed to originate from the old japanese variety 'akakomugi' and have been distributed throughout the world by diverse breeding programmes. twelve microsatellite loci previously mapped on wheat chromosome 2d were used for a retrospective analysis of 59 whea ... | 2002 | 12582874 |
| plasmon analysis of triticum (wheat) and aegilops. 2. characterization and classification of 47 plasmons based on their effects on common wheat phenotype. | this article comprises our final remarks on the phenotypic effects of alien plasmons on common wheat. twenty-one vegetative, reproductive, and seed characters of 551 alloplasmic lines of 12 common wheat genotypes with 46 alloplasmons, and as the control, their euplasmic lines were investigated. effects of genotype, plasmon, and their interaction had high statistical significance for all the characters investigated, whereas phenotypic variations attributable to the alien plasmons were relatively ... | 2002 | 12589076 |
| bioregulation of carbohydrate metabolism in relation to source-sink operation during grain-filling phase of growth in wheat. | mobilization of free sugars from vegetative tissues to grain and their transformation to starch in relation to activities of some relevant enzymes during growth and development were investigated in wheat (triticum aestivum l.). vegetative tissues, viz. flag-leaf, flag-leaf sheath, nodes and internodes contained high concentration of free sugars from 70 das to 18 dpa and that was in the order of accumulation--flag-leaf sheath> flag-leaf and internodes > nodes. in these tissues, major portion of 1 ... | 2002 | 12587738 |
| effect of weed extracts on seedling growth of some varieties of wheat. | allelopathic effect ofavena fatua l., cyperus rotundus l., polygonum hydropiper l., and solanum nigrum l. were examined on seedling growth of certain commonly used varieties of wheat (triticum aestivum l.) in the tarai region of u.p. state. the weed extracts inhibited the length of plumule in all the varieties (100%) with solanum and it was in 12 (92%), 10 (77%) and 06 (46%) varieties with polygonum, avena and cyperus, respectively. in radicle length, it was in 92% with both polygonum and solanu ... | 2002 | 12617313 |
| assessment of the pulp and paper mill effluent on growth, yield and nutrient quality of wheat (triticum aestivum l.). | assessment of agropotentiality of the effluent coming out from century pulp and paper mill, ghanshyamdham, lalkua (uttaranchal) has been made on wheat (triticum aestivum var. up-2329) crop grown in two soils differing in texture with different effluent concentrations. diluted effluent increased the chlorophyll content, plant height, shoot and root biomass, grain yield, protein, carbohydrate and lipid contents in wheat grains, while undiluted effluent caused inhibition in plant growth resulting i ... | 2002 | 12597573 |
| effect of external electrical field on mobile water fraction and physiological processes in wheat (triticum aestivum) leaves. | experiments were conducted to study the effect of external electric field on physiological processes, moisture content and mobile water fraction in wheat leaves. application of electric field increased the stomatal resistance immediately and it continued to increase upto 10 min. photosynthesis and respiration of leaves decreased with the application of external electric field. leaf moisture content and mobile fraction of water measured as relaxation times were marginally increased with electric ... | 2002 | 12597031 |
| isolation of est-derived microsatellite markers for genotyping the a and b genomes of wheat. | genetic variation present in 64 durum wheat accessions was investigated by using three sources of microsatellite (ssr) markers: est-derived ssrs (est-ssrs) and two sources of ssrs isolated from total genomic dna. out of 245 ssr primer pairs screened, 22 est-ssrs and 20 genomic-derived ssrs were polymorphic and used for genotyping. the est-ssr primers produced high quality markers, but had the lowest level of polymorphism (25%) compared to the other two sources of genomic ssr markers (53%). the 4 ... | 2002 | 12582712 |
| spelt-specific alleles in hmw glutenin genes from modern and historical european spelt ( triticum spelta l.). | a partial promoter region of the high-molecular weight (hmw) glutenin genes was studied in two wheat specimens, a 300 year-old spelt ( triticum spelta l.) and an approximately 250 year-old bread wheat ( triticum aestivum l.) from switzerland. sequences were compared to a recent swiss landrace t. spelta'oberkulmer.' the alleles from the historical bread wheat were most similar to those of modern t. aestivumcultivars, whereas in the historical and the recent spelt specific alleles were detected. p ... | 2002 | 12582705 |
| physical location of a hsp70 gene homologue on the centromere of chromosome 1b of wheat ( triticum aestivum l.). | cereal centromeres consist of a complex organization of repetitive dna sequences. several repetitive dna sequences are common amongst members of the triticeae family, and others are unique to particular species. the organization of these repetitive elements and the abundance of other types of dna sequences in cereal centromeres are largely unknown. in this study, we have used wheat-rye translocation lines to physically map 1bl.1rs centromeric breakpoints and molecular probes to obtain further in ... | 2002 | 12582685 |
| production of a new wheat line possessing the 1bl.1rs wheat-rye translocation derived from korean rye cultivar paldanghomil. | the 1bl.1rs translocations between wheat ( triticum aestivum l.) and rye ( secale cereale l.) are widely used in bread wheat breeding programs, but all modern wheat cultivars with the 1bl.1rs have shown genetic vulnerability due to one rye source - a german cultivar, petkus. we have developed, a new 1bl.1rs wheat-rye translocation line from the backcross of the f(1) hybrid of wheat cv. olmil and rye cv. paldanghomil, both cultivars from korea. the gish technique was applied to identify the prese ... | 2002 | 12582683 |
| genetic analysis of vrn-b1 for vernalization requirement by using linked dcaps markers in bread wheat ( triticum aestivum l.). | to identify a molecular marker closely linked to vrn-b1, the vrn-1 ortholog on chromosome 5b, sequence polymorphism at four orthologous rflp loci closely linked to the vrn-1 gene family was analyzed by using near-isogenic lines of "triple dirk." at xwg644, a rflp locus, three types of nucleotide sequence differing by the number of (tg) repeats, two or three times, and base changes were detected. a (tg)(3)-type sequence proved to be specific to chromosome 5b by nulli-tetrasomic analysis, and subs ... | 2002 | 12582660 |
| [mechanism and universality of yoda's -3/2 law of self-thinning in plant population]. | according to plant modular theory, the yoda's-3/2 law of self-thinning was theoretically rederived in this paper. with the parameters in the expression of yoda's-3/2 law being given biological meanings, this article offered universal and rational explanations for relevant data in literatures that could not be explained before. based on the authors' hypothesis, the experiments on the self-thinning process of spring wheat (triticum aestivum l.) were conducted. individual morphological and populati ... | 2002 | 12561166 |
| [effect of clipping at seedling stage on growth and yield of spring wheat]. | the compensation for spring wheat (triticum aestivum) under simulated herbivory stress by clipping was examined in a semi-arid region of gansu province in 1996. the results showed that clipping at seedling stage reduced spring wheat growth under two irrigation conditions. the yield of spring wheat generally under-compensated the clipping effect. heavy clipping (cutting all leaves above the ground, t0, t1) resulted in a more serious reduction in yield, compared with light clipping cutting half of ... | 2002 | 12222043 |
| combinatorial labelling of dna probes enables multicolour fluorescence in situ hybridisation in plants. | this paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (fish). three different dna probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of secale cereale and triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single fish experiment. combinatorial labelling can augment karyotypical investigations, physical mapping of chr ... | 2002 | 12219843 |
| flow cytometric analyses of intraplant nuclear dna content variation induced by sticky chromosomes. | in several plant species, sticky chromosomes are a consequence of genetic mutations or environmental effects on mitosis and meiosis. sticky chromosomes result in an unequal distribution of genetic material in daughter cells. this unequal distribution is hypothesized to result in an increase in the coefficient of variation (cv) of the g1 peak of dividing cells. | 2002 | 12210609 |
| transport interactions between cadmium and zinc in roots of bread and durum wheat seedlings. | field studies have shown that the addition of zn to cd-containing soils can help reduce accumulation of cd in crop plants. to understand the mechanisms involved, this study used 109cd and 65zn to examine the transport interactions of zn and cd at the root cell plasma membrane of bread wheat (triticum aestivum l.) and durum wheat (triticum turgidum l. var. durum). results showed that cd2+ uptake was inhibited by zn2+ and zn2+ uptake was inhibited by cd2+. concentration-dependent uptake of both cd ... | 2002 | 12207664 |
| mapping quantitative trait loci for plant height in wheat (triticum aestivum l.) using a f2:3 population. | to detect quantitative trait loci (qtls) controlling plant height, the plant height of 240 f2:3 lines derived from the cross of a dwarf wheat line nd3338 with a tall line f390, was assessed in field trials at two locations with three replications in 2000 and 2001. microsatellite markers were used to construct a framework linkage map containing 215 loci with 21 linkage groups, and covering the whole genome about 3600cm. with the method of interval mapping, seven putative qtls affecting plant heig ... | 2002 | 12200862 |
| [identification of blue grained wheat and its irradiation-mutated offsprings by genomic in situ hybridization (gish)]. | transferring useful chromosome(s), chromosomal fragment(s) or genes from related species into common wheat (triticum aestivum l.) is an effective method for improving wheat cultivars, and dna:dna in situ hybridization has been proven to be an effective method for directly demonstrating alien chromosome number and distribution in plant. in this study, genomic in situ hybridization (gish) was used to identify the chromosomal constitutions of the blue-grained wheat blue-58 originated from distant h ... | 2002 | 12182082 |
| ca(2+)-induced high amplitude swelling and cytochrome c release from wheat (triticum aestivum l.) mitochondria under anoxic stress. | under stress conditions, mitochondria sense metabolic changes, e.g. in ph, cytoplasmic ca(2+), energy status, and reactive oxygen species (ros), and respond by induction of the permeability transition pore (ptp) and by releasing cytochrome c, thus initiating the programmed cell death (pcd) cascade in animal cells. in plant cells, the presence of all the components of the cascade has not yet been shown. in wheat (triticum aestivum l.) root mitochondria, the onset of anoxia caused rapid dissipatio ... | 2002 | 12324275 |
| interaction between drought and chronic high temperature during kernel filling in wheat in a controlled environment. | wheat plants (triticum aestivum l. 'lyallpur'), limited to a single culm, were grown at day/night temperatures of either 18/13 degrees c (moderate temperature), or 27/22 degrees c (chronic high temperature) from the time of anthesis. plants were either non-droughted or subjected to two post-anthesis water stresses by withholding water from plants grown in different volumes of potting mix. in selected plants the demand for assimilates by the ear was reduced by removal of all but the five central ... | 2002 | 12324270 |
| transfer of rye chromosome segments to wheat by a gametocidal system. | a gametocidal chromosome derived from aegilops triuncialis (3c) induces chromosome mutations in gametes lacking the 3c chromosome in common wheat (triticum aestivum l.). we combined 3c with chromosome 1r of rye (secale cereale l.) in a common wheat line to know how efficiently 3c induces transfers of small 1r segments to wheat. in the 811 progeny of this wheat line, we found five wheat chromosomes (2a, 2d, 3d, 5d and 7d) carrying segments of the 1r satellite. wheat plants carrying these transloc ... | 2002 | 12296517 |
| a germination-related gene encoding a serine carboxypeptidase is expressed during the differentiation of the vascular tissue in wheat grains and seedlings. | carboxypeptidases expressed in the aleurone layer participate in the mobilization of endosperm storage proteins during cereal grain germination. the genes encoding these proteins are also expressed in the scutellum of germinating grains, but their function in this organ is not yet clear. we have analyzed the expression of a carboxypeptidase iii (cpiii) gene in germinating wheat (triticum aestivum l.) grains. cpiii transcripts accumulated transiently in the scutellum showing a maximum at 2-3 days ... | 2002 | 12244437 |
| transgenic wheat plants resistant to herbicide basta obtained by microprojectile bombardment. | wheat (triticum aestivum) transgenic plants of an important commercial cv (oasis) was obtained with an efficient and short procedure. first, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. the transformation of calli was performed by high velocity microprojectile bombardment, using the pahc25 plasmid, which contains the reporter gene beta-glucuronidase (gus) and the selectable bar gene which confers resistanc ... | 2002 | 12240555 |
| [reorganization of the tubulin and actin cytoskeleton under acclimation and abscisic acid treatment of triticum aestivum l. plants]. | only scanty and contradictory data are available concerning effects of low temperatures and aba on the structural organization of microtubules (mts) and microfilaments (mfs), and no information exists on the interaction of these parameters at cold acclimation of plants. therefore, in cold acclimate and aba-treated winter wheat plants, a comparative study was made of the state (localization, orientation, structure) and stability of actin and tubulin cytoskeleton in root cells taken from different ... | 2002 | 12236096 |
| arabinoxylan biosynthesis in wheat. characterization of arabinosyltransferase activity in golgi membranes. | arabinoxylan arabinosyltransferase (ax-arat) activity was investigated using microsomes and golgi vesicles isolated from wheat (triticum aestivum) seedlings. incubation of microsomes with udp-[(14)c]-beta-l-arabinopyranose resulted in incorporation of radioactivity into two different products, although most of the radioactivity was present in xylose (xyl), indicating a high degree of udp-arabinose (ara) epimerization. in isolated golgi vesicles, the epimerization was negligible, and incubation w ... | 2002 | 12226522 |
| tandemly duplicated safener-induced glutathione s-transferase genes from triticum tauschii contribute to genome- and organ-specific expression in hexaploid wheat. | glutathione s-transferase (gst) gene expression was examined in several triticum species, differing in genome constitution and ploidy level, to determine genome contribution to gst expression in cultivated, hexaploid bread wheat (triticum aestivum). two tandemly duplicated tau class gst genes (ttgstu1 and ttgstu2) were isolated from a single bacterial artificial chromosome clone in a library constructed from the diploid wheat and d genome progenitor to cultivated wheat, triticum tauschii. the ge ... | 2002 | 12226515 |
| genus-specific localization of the taii family of tandem-repetitive sequences in either the centromeric or subtelomeric regions in triticeae species (poaceae) and its evolution in wheat. | the taii family sequences are classified as tandem repetitive dna sequences present in the genome of tribe triticeae, and are localized in the centromeric regions of common wheat, but in the subtelomeric heterochromatic regions of leymus racemosus and related species. in this study, we investigated the chromosomal distribution of taii family sequences in other triticeae species. the results demonstrated a centromeric localization in genera triticum and aegilops and subtelomeric localization in o ... | 2002 | 12416628 |
| [screening for resistance gene candidate from a genomic tac library of triticum aestivum-haynaldia villosa translocation line 6vs/6al by pooled pcr]. | a pair of degenerate primers were designed based on nbs (nucleotide binding site, nbs) domain of resistance(r) gene and used to perform pcr with cdna from the translocation line 6vs/6al of triticum aestivum-haynaldia villosa. a clone (n7) characterized with nbs was obtained by sequencing analysis. two specific primers were designed from the n7 sequence and used to screen a genomic tac (transformation-competent artificial chromosome, tac) library of 6vs/6al consisting of ca. 2 x 10(6) clones. the ... | 2002 | 12192864 |
| non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is post-translationally phosphorylated in heterotrophic cells of wheat (triticum aestivum). | in wheat, non-phosphorylating, nadp-dependent glyceraldehyde-3-phosphate dehydrogenase (gapn) was found to be encoded by one gene giving rise to a single protein. however, western blots revealed two different subunits of about 58 and 60 kda in endosperm and shoots. the latter was attributed to in vivo phosphorylation of shoot gapn. no modification occurred in leaves, where the enzyme is composed by a single 58 kda polypeptide. gapn partially purified from shoots and endosperm was dephosphorylate ... | 2002 | 12387887 |
| germination and growth inhibitors from wheat (triticum aestivum l.) husks. | on the basis of our findings that the germination of intact wheat seeds (with husks) belonging to dormancy varieties was restrained as compared with that of the dehusked seeds (grains), the germination inhibitors in the husks were explored. the water-soluble extracts from the husks were separated by the aid of inhibition assay experiments, resulting in the characterization of 2-phenylethyl alcohol 1, 4-vinylphenol 2 and its 2-methoxy derivative 3, and dihydroactinidiolide 4, all of which showed ... | 2002 | 12381108 |
| semi-specific pcr for the evaluation of diversity among cultivars of wheat and triticale. | the usefulness of a semispecific pcr system for the evaluation of genetic diversity among selected cultivars of wheat (triticum aestivum l.) and triticale (x triticosecale wittm.) was investigated. a comparison was made of the diversity among cultivars of both species as revealed by primers targetting the semi-conservative sequences of the intron-exon junction. the main purpose of the study was the selection of the most informative primers from among the exon targetting (et) and intron targettin ... | 2002 | 12378263 |
| the abscisic acid-responsive kinase pkaba1 interacts with a seed-specific abscisic acid response element-binding factor, taabf, and phosphorylates taabf peptide sequences. | the abscisic acid (aba)-induced protein kinase pkaba1 is present in dormant seeds and is a component of the signal transduction pathway leading to aba-suppressed gene expression in cereal grains. we have identified a member of the aba response element-binding factor (abf) family of basic leucine zipper transcription factors from wheat (triticum aestivum) that is specifically bound by pkaba1. this protein (taabf) has highest sequence similarity to the arabidopsis aba response protein abi5. in two ... | 2002 | 12376648 |
| effects of lanthanum and cerium on the vegetable growth of wheat (triticum aestivum l.) seedlings. | 2002 | 12375123 | |
| investigation and correction of the gene-derived sequence of glutenin subunit 1dx2 by matrix-assisted laser desorption/ionisation mass spectrometry. | direct matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tofms) analysis of a mixture of tryptic peptides was used to verify the gene-derived amino acid sequence of the high molecular weight (hmw) subunit 1dx2 of bread wheat. analysis of the digest was performed by recording several maldi mass spectra of the mixture at low, medium and high mass ranges, and optimising the matrix and the acquisition parameters for each mass range. this resulted in coverage of the ... | 2002 | 12362381 |
| characterisation of polymorphic microsatellite markers from aegilops tauschii and transferability to the d-genome of bread wheat. | microsatellites were isolated from a aegilops tauschii (the d-genome donor of bread wheat) library enriched for various motifs. primers generated from the flanking region of the microsatellites were used successfully to amplify the corresponding loci in the d genome of bread wheat. additional amplification sometimes also occurred from the a and b genomes. the majority of the microsatellites contained (ga)(n) and (gt)(n) motifs. ga and gt repeats appeared to be both more abundant in this library ... | 2002 | 12582627 |
| comparison of homoeologous group-6 short arm physical maps of wheat and barley reveals a similar distribution of recombinogenic and gene-rich regions. | eighty two new loci, mapped with 51 dna clones, were added to the earlier deletion maps of the homoeologous group-6 short arms of hexaploid wheat ( triticum aestivum l. em thell., 2n = 6 x = 42, aabbdd). there are now 41, 56 and 52 loci mapped on deletion maps of 6as, 6bs and 6ds, respectively. the linear order of orthologous loci in all three arms appears to be identical. the majority of the loci are located in the distal one-half of the three arms. there seems to be an increased marker/gene de ... | 2002 | 12582615 |
| genes responding to vernalization in hexaploid wheat. | genotype-specific gene expression in response to vernalization in common wheat was examined by the differential display method. two near-isogenic lines of vrn-a1 ( vrn-a1 for the spring type and vrn-a1 for the winter type) were treated by vernalization of developing embryos in detached-ear cultures. this treatment was effective to promote vrn-a1 genotypes to head at a time equivalent to that of vrn-a1. differential cdna fragments were isolated by the rt-pcr method from embryos subjected to verna ... | 2002 | 12582606 |
| flow karyotyping and chromosome sorting in bread wheat ( triticum aestivum l.). | previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. that study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. however, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3b could be sorted into a high-purity fraction. the present study aimed to identify wheat stocks ... | 2002 | 12582592 |
| identification of novel low m(r) glutenin subunits in the high quality bread wheat cv salmone and their effects on gluten quality. | southern-blot hybridization with a probe specific for genes encoding for low m(r) glutenin subunits showed that the high quality bread wheat cv salmone contains two dna fragments designated as sf720 and sf750. these fragments were found to occur on the chromosome-1b satellite, and to be associated with the presence of two strongly staining low m(r) glutenin subunits in the two-dimensional a-page x sds-page pattern of cv salmone. comparison of 65 f(6) random lines derived from the cross between c ... | 2002 | 12582560 |
| mixing of maize and wheat genomic dna by somatic hybridization in regenerated sterile maize plants. | intergeneric somatic hybridization was performed between albino maize ( zea mays l.) protoplasts and mesophyll protoplasts of wheat ( triticum aestivum l.) by polyethylene glycol (peg) treatments. none of the parental protoplasts were able to produce green plants without fusion. the maize cells regenerated only rudimentary albino plantlets of limited viability, and the wheat mesophyll protoplasts were unable to divide. peg-mediated fusion treatments resulted in hybrid cells with mixed cytoplasm. ... | 2002 | 12582555 |
| genetic mapping of 66 new microsatellite (ssr) loci in bread wheat. | in hexaploid bread wheat ( triticum aestivum l. em. thell), ten members of the iwmmn ( international wheat microsatellites mapping network) collaborated in extending the microsatellite (ssr = simple sequence repeat) genetic map. among a much larger number of microsatellite primer pairs developed as a part of the wmc ( wheat microsatellite consortium), 58 out of 176 primer pairs tested were found to be polymorphic between the parents of the itmi ( international triticeae mapping initiative) mappi ... | 2002 | 12582546 |
| studies of the transferability of microsatellites derived from triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. | hexaploid wheat ( triticum aestivum l em thell) is derived from a complex hybridization procedure involving three diploid species carrying the a, b and d genomes, respectively. we recently isolated microsatellites from a t. tauschii library enriched for various motifs and evaluated the transferability of these markers to several diploid species carrying the a, b or d genomes. all of the primer pairs amplifying more than one locus on bread wheat and half of those giving d-genome-specific loci gav ... | 2002 | 12582487 |
| assessing genetic diversity of wheat ( triticum aestivum l.) germplasm using microsatellite markers. | a set of 24 wheat microsatellite markers, representing at least one marker from each chromosome, was used for the assessment of genetic diversity in 998 accessions of hexaploid bread wheat ( triticum aestivum l.) which originated from 68 countries of five continents. a total of 470 alleles were detected with an average allele number of 18.1 per locus. the highest number of alleles per locus was detected in the b genome with 19.9, compared to 17.4 and 16.5 for genomes a and d, respectively. the l ... | 2002 | 12582483 |
| study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a whitexred grain bread-wheat cross. | in many wheat ( triticum aestivuml.) growing areas, pre-harvest sprouting (phs) may cause important damage, and in particular, it has deleterious effects on bread-making quality. the relationship between phs and grain color is well known and could be due either to the pleiotropic effect of genes controlling red-testa pigmentation ( r) or to linkage between these genes and other genes affecting phs. in the present work, we have studied a population of 194 recombinant inbred lines from the cross b ... | 2002 | 12579426 |
| a strategy for enhancing recombination in proximal regions of chromosomes. | as a rule, recombination in bread wheat (triticum aestivum l.) is low in proximal and high in distal regions of chromosomes. recombination may be enhanced in proximal regions by using deletion (del) chromosomes deficient for a distal part of a chromosome arm. the chromosome del5bl-11 derived from chinese spring (cs) is missing 41% of the distal long arm. this line was made polymorphic by crossing with a stock in which chromosome 5b of cs (5b(cs)) is substituted for chromosome 5b of t. turgidum s ... | 2002 | 12575793 |
| [molecular analysis of leaf-rust resistant introgression lines obtained by crossing hexaploid wheat triticum aestivum with tetraploid wheat triticum timopheevii]. | twenty-four triticum eastivum x t. timopheevii hybrid lines developed on the basis of five varieties of common wheat and resistant to leaf rust were analyzed by the use of microsatellite markers specific for hexaploid common wheat t. aestivum. investigation of intervarietal polymorphism of the markers showed that the number of alleles per locus ranged from 1 to 4, depending on the marker (2.5 on average). in t. timopheevii, amplification fragments are produced by 80, 55, and 30% of primers speci ... | 2002 | 12575450 |
| molecular analysis of the barley ( hordeum vulgare l.) gene encoding the protein kinase pkaba1 capable of suppressing gibberellin action in aleurone layers. | abscisic acid (aba) induces the expression of a gene encoding a protein kinase, pkaba1, in wheat ( triticum aestivum l.). transient expression of the cdna encoding this kinase suppresses the gibberellin (ga)-induced expression of alpha-amylase genes in barley ( hordeum vulgare l.) aleurone layers. we have cloned the cdna and genomic sequences for the barley pkaba1 (hvpkaba1) to further study its role in regulating the ga-inducible gene expression in barley seeds. the amino acid sequence deduced ... | 2002 | 12029482 |
| genomic targeting and high-resolution mapping of the domestication gene q in wheat. | the q locus is largely responsible for the domestication of bread wheat. q confers the free-threshing character of the spike and influences other important agronomic traits. using chromosome deletion lines, q was placed on the physical map within a submicroscopic segment of the long arm of chromosome 5a. we targeted markers to the segment by comparative mapping of anonymous rflp clones, aflp, and mrna differential display analysis of deletion lines 5al-7 and -23, which have deletion breakpoints ... | 2002 | 12175074 |
| assessment of genomic diversity among wheat genotypes as determined by simple sequence repeats. | simple sequence repeats (ssrs) have been used to examine the genomic diversity of wheat (triticum aestivum l.) germplasm. thirteen wheat genotypes of diverse origin were analyzed with 43 selected ssrs to provide uniform and maximum genome coverage. a total of 156 allelic variants were detected at 43 ssr loci, ranging from two to eight per locus with an average of 3.6. the polymorphic information content (pic) values of the loci ranged from 0.10 (xgwm264) to 0.89 (xgwm471 and xgwm577). genetic si ... | 2002 | 12175067 |
| assessment of plant uptake of radioactive nickel from soils. | as a result of isotopic dilution, the availability to plants of radioisotopes introduced into the soil solution should be directly related to the size of the isotopically exchangeable pool (e(t))-value). this work was undertaken to test this hypothesis for the radionuclide 63ni. the demonstration was based on pot experiments conducted with seven soils representing a large range of ni content (from 9.9 mg kg(-1) to 862.6 mg kg(-1)) which were mixed with a 63nicl2 solution (100 kbq kg(-1)). three ... | 2002 | 12171471 |
| differential stress responses of early salt-stress responding genes in common wheat. | four early salt-stress responding genes (wesr1-4) in common wheat (triticum aestivum l.) were analyzed for their temporal accumulation of mrna during salt stress, osmotic stress and abscisic acid (aba) treatment. all genes showed transient stimulation by 0.15 m nacl treatment. wesr1 and wesr2 were induced by both osmotic stress and exogenous aba treatment. wesr3 responded to exogenous aba, but not to osmotic stress. wesr4 did not show significant response to either osmotic stress or exogenous ab ... | 2002 | 12169305 |