Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| viruses and pathogenicity of entamoeba histolytica. | 1984 | 6147594 | |
| viruses and the pathogenicity of entamoeba histolytica. | 1984 | 6148562 | |
| cytopathogenicity of entamoeba histolytica. | the lesions induced in man by entamoeba histolytica are characterized by massive tissue injury in the absence of major local signs of a host immune response. the amoeba damages surrounding cells preferentially by contact-mediated cytolysis. recently, a presumptive aetiological factor underlying this process has been identified. it is a protein, amoebapore, capable of spontaneous incorporation into host cell membranes. therein it induces high conductance ion-channels which rapidly collapse the ce ... | 1984 | 6151690 |
| [epidemiological studies on protozoan infection in gyeong-gi do and jeonra bug do] | the present study was designed to elucidate the recent prevalence of the intestinal protozoa and to demonstrate the relationship on prevalence of the surveyed population with their academic career and living conditions. during the period from may to july 1981, total 2,735 fecal specimens were collected from the inhabitants of 11 areas in gyeong-gi do (province) near by seoul city, and 6 areas in jeonra bug do, southwestern part of korea. the samples were fixed with saf solution and were examined ... | 1984 | 12891041 |
| cellulose acetate precipitation test in sero-diagnosis of amoebiasis. | cellulose acetate precipitation (cap) test for the detection of antibody against entamoeba histolytica using the axenic antigen, was performed on the 127 serum samples obtained from patients with amoebic liver abscess (14), amoebic hepatitis (21), amoebic dysentry (11), amoebic colitis (31), other parasitic infestations (25) and normal individuals (25). the percent positivity was 100, 95.23, 90.9, 67.74 and 16 and 12 respectively whereas the corresponding figures for the indirect immunoflouresce ... | 1984 | 12055791 |
| [changes in the pathogenicity of naegleria fowleri by several brain passage in mice] | the pathogenicity of free-living amoeba, naegleria fowleri, is influenced according to the strain, cultural condition and host (culbertson et al., 1968; carter, 1970; wong et al., 1975). phillips (1973) demonstrated that entamoeba histolytica became avirulent after more than 2 year maintenance in axenic culture in vitro. this study was carried out to compare the difference in pathogenicity between two strains of n. fowleri, one of a prolonged maintenance in axenic medium and the other one obtain ... | 1983 | 12902654 |
| susceptibility testing of entamoeba histolytica. | the growth of entamoeba histolytica in microtiter plates in vitro in a variety of environments with reduced oxygen tensions is reported. with 3% o2, 3% co2, and 94% n2, the parasite growth in microtiter plates was identical to that in screw-capped culture tubes, as measured by [3h]thymidine incorporation and by quantitative parasite counts. there were no significant differences between the drug concentrations necessary to inhibit parasite growth by 50% based on [3h]thymidine incorporation vs tho ... | 1983 | 6140291 |
| selective primary health care: strategies for control of disease in the developing world. x. amebiasis. | the human parasite entamoeba histolytica has a worldwide distribution. the majority of infected individuals suffer few symptoms; nonetheless, a variable proportion experience dysentery, hepatic abscesses, or invasion of other extraintestinal organs. invasive amebiasis is a major health problem, particularly in certain developing countries. the aim of this review is to clarify, as much as possible, controversial issues about the biology of the parasite, the clinical features of amebiasis, and the ... | 1983 | 6140740 |
| [possible participation of bacteria in the formation of surface-active centers on the trophozoite of the dysentery ameba]. | 1983 | 6195515 | |
| hepatic amoebiasis. current concepts and a report of 25 cases in the netherlands. | 1983 | 6089489 | |
| [comparison of 4 methods for the detection of entamoeba histolytica in stool]. | 1983 | 6088118 | |
| [intestinal parasites in foreigners entering east germany]. | among the so-called imported diseases the intestinal parasitoses occupy an important place. examinations of 5,168 foreigners who entered our country in the district of halle resulted in the most frequent invasiveness for trichuris trichiura (whipworm) with 54.33%, ascaris lumbricoides (eel-worm) with 26.90% and ancylostomatides (hookworms) with 17.88%. the entamoeba histolytica could be proved in 1.90% of the test persons, the giardia lamblia in 3.35%. the severest parasitism showed persons from ... | 1983 | 6613197 |
| the incidence of amoebiasis among secondary school students in calabar, nigeria. | a study was conducted among 2 500 secondary school students in calabar, in order to determine the incidence of amoebiasis caused by entamoeba histolytica. microscopic examination of faecal sample smears showed higher percentage infections in the males than in the females; 61 out of 1 250 males were infected (4.8%), while 52 of 1 250 females were infected (4.1%). among infected persons, asymptomatic carriers (64.6%) and symptomatic cases (35.4%) were recorded. infection was lower among students u ... | 1983 | 6629207 |
| natural entamoeba histolytica infection in domestic animals and rodents in dakahlia governorate, egypt. | 1983 | 6631088 | |
| the adverse consequences of heavy trichuris infection. | sixty-seven children with heavy trichuris trichiura infection (group a) were compared to 73 control children of similar socio-economic status but with light or no t. trichiura (group b), for nutritional status, rates of concomitant bacterial and protozoal, and symptoms and clinical signs associated with heavy t. trichiura infection. anoscopy was used to determine heavy t. trichiura infection. measurements and physical examination were done on group a on admission to and discharge from hospital; ... | 1983 | 6636270 |
| phase 1 tolerability and antiamoebic activity studies with 1-methylsulphonyl-3-(1-methyl-5-nitro-2-imidazolyl)-2-imidazolidinone (go.10213): a new antiprotozoal agent. | go.10213, a new nitroimidazole, was studied in 12 male volunteers for tolerability and in 20 patients with intestinal amoebiasis for antiamoebic activity. go.10213 was well-tolerated by volunteers up to a dose of 400 mg x 3. patients also tolerated well the dose of 100-150 mg x 3 for 7 days. in two patients, there were mild and transient side-effects like headache, dizziness, fatigue, etc. no neurological side-effects were observed. there were no significant changes in blood pressure, pulse rate ... | 1983 | 6639838 |
| role of macrophages in host defense against hepatic amoebiasis in hamsters. | the role of macrophages in hepatic amoebiasis in hamsters has been investigated by means of antimacrophage serum prepared in rabbits. animals treated with normal rabbit serum or antimacrophage serum, as well as untreated controls, were inoculated intrahepatically with 10(5) axenic trophozoites of entamoeba histolytica. in hamsters treated with antimacrophage serum before intrahepatic inoculation of amoebae, the mean weight of the metastatic foci was significantly greater than in normal rabbit se ... | 1983 | 6642657 |
| induction of lung tumors and lymphomas in balb/c mice by metronidazole. | metronidazole, which is widely used in the treatment of "trichomonas vaginalis", "entamoeba histolytica" and "giardia lamblia" infections, was administered to balb/c mice by stomach tube in an aqueous solution at a dose rate of 2 mg/day for 100 days (total 200 mg) to test its carcinogenicity. the treatment induced a significant increase in lung tumors in male mice (p less than 0.001) and provoked the appearance of lymphomas in females (p less than 0.001). although there is insufficient evidence ... | 1983 | 6649066 |
| treatment of chronic amebiasis in pediatric patients with a suspension of quinfamide. | quinfamide, a dichloroacetyl quinolol synthesized and tested at sterling winthrop research institute, is a potent luminal amebicide with potential utility for a one-day treatment of chronic and subacute amebiasis caused by entamoeba histolytica. previous studies demonstrated that quinfamide is a safe and efficacious drug for adult patients when given as a one-day treatment regimen of 300 mg taken in tablet form at a dosage of 100 mg every eight hours. to test the drug in suspension form in pedia ... | 1983 | 6673831 |
| a study of the incidence of porcine taeniasis and other intestinal parasites in a rural community of south western nigeria. | in the course of an investigation into the incidence of porcine taeniasis among humans and and pigs which live in close contact with each other in a rural town of south-western nigeria, no incidence of the parasite was found. ascaris, strongyle (hookworm in case of humans) and trichuris were the helminths encountered while entamoeba histolytica and entamoeba coli were the common protozoan cysts. polyparasitism was common and a 12 year old boy had salmonella infection concurrently with ascaris an ... | 1983 | 6685713 |
| cytopathologic diagnosis of extracolonic amebiasis. | extracolonic amebiasis must be distinguished from bacterial abscesses and neoplastic disease. newer technology in diagnostic and interventional radiology permits such lesions to be aspirated more accurately for both diagnostic and therapeutic purposes, often averting an open surgical procedure. paracecal and hepatic masses of one patient, who presented with fever and abdominal pain, were shown to be amebic abscesses by cytopathologic examination of material obtained by ultrasonically guided perc ... | 1983 | 6580801 |
| the activity of fexinidazole (hoe 239) against experimental infections with trypanosoma cruzi, trichomonads and entamoeba histolytica. | a new 5-nitroimidazole compound, 1-methyl-2-(4-methylthiophenoxymethyl)-5-nitroimidazole: fexinidazole, hoe 239, was found to be highly active against experimental infections with trypanosoma cruzi, tritrichomonas foetus, trichomonas vaginalis and entamoeba histolytica. it was slightly superior to benznidazole and nifurtimox in suppressing parasitaemia in mice infected with t. cruzi. it also eradicated the parasites from infected mice after a prolonged treatment period. the order of activity of ... | 1983 | 6411009 |
| micro-counterimmunoelectrophoresis: a rapid screening technique for trypanosomiasis. | a rapid and economical micro-counterimmunoelectrophoresis (mcie) test, for antibody to trypanosome antigens, has been developed which may prove useful in trypanosomiasis surveillance. mcie was more sensitive and more rapid than immunodiffusion (id). serum samples from trypanosome-infected rabbits and cattle (nigerian and gambian), from kuwaiti dogs and camels, from ivory coast sleeping sickness patients, from brazilian patients with chagas's disease, plus mouse-anti-malaria sera have been tested ... | 1983 | 6415874 |
| the use of the solid phase indirect immunofluorescent assay (fiax) in the serodiagnosis of amebiasis. | 1983 | 6326646 | |
| model of amoebic liver abscess in cholesterol fed guinea pigs through intracaecal infection route. | 1983 | 6321341 | |
| action of antiamoebic drugs on hepatic microsomal drug-metabolizing enzymes of hamster infected with virulent entamoeba histolytica. | 1983 | 6325336 | |
| evaluation of three culture media for the isolation of entamoeba histolytica. | 1983 | 6325531 | |
| identification of stocks of non-pathogenic "entamoeba histolytica". | the isoenzyme mobilities, on thin layer starch gel electrophoresis, of stocks of entamoeba histolytica have been shown to relate to pathogenicity in the host. the ability of some zymodemes of this organism to produce extra-intestinal lesions is also coupled with a capacity to resist lysis by human serum. the results of isoenzyme characterization particularly related to serum lysis is discussed. | 1983 | 6325031 |
| a seroepidemiological survey of amoebiasis in cdri staff using iha, ifa, elisa, and ai tests by taking a drop of peripheral blood. | 1983 | 6323570 | |
| a new amebicide agent from castela texana. | 1983 | 6310633 | |
| human milk kills parasitic intestinal protozoa. | giardia lamblia, a common pathogenic intestinal parasite of humans, was rapidly killed by exposure to normal human milk in vitro. the killing did not depend on secretory immunoglobulin a. entamoeba histolytica, the dysentery amoeba, was also killed by normal human milk. giardia-cidal activity cochromatographed with an unusual lipase that is present in the milk of humans but not of lower mammals. human milk may play a protective role in infants exposed to this parasite. | 1983 | 6310751 |
| [cutaneous amebiasis: culture and enzymatic characterization of a strain]. | 1983 | 6311230 | |
| [sexually transmitted intestinal pathogens among homosexual men in stockholm]. | 1983 | 6312221 | |
| latency and heterogeneity of entamoeba histolytica hemolysins. | we have found that the hemolytic potency of p30 - a subcellular fraction that contains most of the hemolytic activity of entamoeba histolytica trophozoites--increased up to 100 times by preincubation for 36 h at 36 degrees c. this remarkable increase in potency was due to the in vitro generation of heat-labile as well as heat-stable hemolytic components and could be prevented if p30 was preincubated at 4 degrees c or subjected to either heating at 90 degrees c or to repeated freeze-thawing cycle ... | 1983 | 6312705 |
| absence of alpha-glycerophosphate dehydrogenase in axenically grown entamoeba histolytica. | we conclude that there is no evidence for the presence of alpha-glycerophosphate dehydrogenase among the cytoplasmic enzymes of entamoeba histolytica, and that a contrary finding was probably caused by the action of a different amebal enzyme on unrecognized contamination in the lithium salt of the substrate used in that investigation. | 1983 | 6312822 |
| the partial purification and characterization of adenosine kinase from entamoeba histolytica. | axenically grown entamoeba histolytica was found to contain adenosine kinase. this organism lacks de novo purine biosynthetic pathways. adenosine kinase provides the amoeba with a method for salvaging adenosine from ingested nucleosides or from degraded nucleotides. adenosine kinase was purified 64-fold, by chromatography on sephacryl s-200, deae-cellulose, and (c-8)-adenosine-agarose. the latter separated it from amebal adenylate kinase. adenosine kinase has a molecular weight of 38,000 and req ... | 1983 | 6312825 |
| comparative studies on the amoebicidal activity of known 5-nitroimidazole derivatives and cg 10213-go in golden hamsters, mesocricetus auratus, infected in the liver or caecum or both with trophozoites of entamoeba histolytica. | cg 10213-go, a 5-nitroimidazole derivative synthesized at the ciba-geigy research centre, bombay, proved superior to such currently used 5-nitroimidazole derivatives as metronidazole, ornidazole, secnidazole, tinidazole and nimorazole, against experimental amoebic infections of the liver and caecum in a golden hamster model. cg 10213-go is now undergoing clinical trials. | 1983 | 6312905 |
| accumulation of 14c-chloroquine by entamoeba histolytica. | 1983 | 6312906 | |
| entamoeba histolytica. phagocytosis as a virulence factor. | in this paper, we attempted to define the role of phagocytosis in the virulence of entamoeba histolytica. we have isolated, from a highly phagocytic and virulent strain, a clone deficient in phagocytosis. trophozoites of wild-type strain hm1:imss were fed with escherichia coli strain cr34-thy- grown on 5-bromo,2'-deoxyuridine. the trophozoites that had incorporated the base analog through phagocytosis of the bacteria were killed by irradiation with 310 nm light. the survivors, presumably trophoz ... | 1983 | 6313842 |
| detection of campylobacter jejuni and other potential pathogens in travellers' diarrhoea in bangladesh. | to determine the distribution of pathogens associated with travellers' diarrhoea in bangladesh, we have studied 269 expatriates who presented with diarrhoea to our clinic, over a one-year period. patients were interviewed about their history of diarrhoea and a stool specimen examined for parasites and cultured for campylobacter jejuni, shigella sp., salmonella sp., vibrio and aeromonas hydrophila and plesiomonas shigelloides. c. jejuni and shigella sp. were each isolated from 45 patients (17%) a ... | 1983 | 6314488 |
| parasites and travellers' diarrhoea. | giardia lamblia is discussed as the most important parasitic cause of travellers' diarrhoea. the parasitology of this flagellate protozoan is discussed with respect to route of infection, threshold infecting dose, and incubation period. the geographic distribution of giardiasis is widespread, being common in tropical and sub-tropical regions and endemic at a low level in european and north american countries. the predisposing factors are detailed together with clinical manifestations, pathogenes ... | 1983 | 6314489 |
| amoebiasis and giardiasis in bangladesh: parasitological and serological studies. | to determine the prevalence of infections with entamoeba histolytica and giardia lamblia in bangladesh, we screened stool specimens from patients with diarrhoea attending urban (n = 2,246) and a rural (n = 2,791) hospital and a group of healthy urban residents (n = 440). sera from 200 healthy villagers were also examined for antibody to e. histolytica using the immunofluorescent antibody test. the prevalence of e. histolytica cysts or trophozoites in all groups assessed by examination of a singl ... | 1983 | 6314613 |
| interactions between entamoeba histolytica, bacteria and intestinal cells. | axenically grown pathogenic and non-pathogenic isolates of entamoeba histolytica have been shown to adhere to mammalian epithelial cells and bacteria by virtue of carbohydrate-binding proteins present on their cell surfaces. the interaction of amoeba isolates of low pathogenicity with a variety of gram-negative bacteria, mainly escherichia coli strains which are readily ingested by the amoebae after relatively short periods, significantly increased the ability of the trophozoites to: (a) destroy ... | 1983 | 6315320 |
| hepatic and gastric amebiasis in black and white colobus monkeys. | three cases of hepatic amebiasis and one case of gastric amebiasis were diagnosed in black and white colobus monkeys during a 9-month period. the diagnosis was difficult because of the absence of trophozoites and cysts in the feces and because of few trophozoites found in many of the hepatic lesions. indirect hemagglutination titers were diagnostic in 2 monkeys. | 1983 | 6315656 |
| amebic liver abscess in a homosexual man. | a case report of a 36-year-old black homosexual man with right upper- and lower-quadrant abdominal pain is presented. when exploratory surgery for appendicitis was performed, an abscess, consistent with an amebic abscess, was encountered in the right lobe of the liver. this abscess was evacuated, and a drain was placed. a serum sample taken at the time of surgery showed high titers of antibody to entamoeba histolytica; metronidazole therapy was instituted, and the patient made an uneventful reco ... | 1983 | 6316572 |
| scanning electron microscopy of attached trophozoites of pathogenic entamoeba histolytica. | the surface morphology of entamoeba histolytica trophozoites of hm 1:imss (axenic and monoxenic) and hk9 (axenic) strains cultured on plastic and mdck cell substrates was examined using scanning electron microscopy (sem). the conditions for processing trophozoites were determined by comparing the sem observations with the morphology of living amebas examined by light microscopy. the most frequent surface differentiations in all the amebas observed with sem were lobopodia. round cytoplasmic proje ... | 1983 | 6319685 |
| [entamoeba histolytica: i. mechanism of cytotoxic activity]. | cytotoxic action against k562-tissue culture cells was investigated under various conditions with a chromium-release-assay. when amoebae and target cells were centrifuged together, pathogenic strains of amoebae induced a very fast increase of target cell lysis (up to 50% of maximum lysis after 10 minutes). only a minor degree of target cell lysis resulted, however, when amoebae and k562 cells were kept in suspension. when amoebae were eliminated selectively by addition of complement 10 minutes a ... | 1983 | 6320511 |
| the role of endoscopy in suspected amebiasis. | the diagnosis of amebic colitis can be difficult and confusing. the gross endoscopic appearance as well as the results of endoscopic biopsy can be extremely helpful in differentiating amebiasis from other forms of colitis. clinical symptoms, laboratory studies, x-ray findings, cultures, and even serological studies may not be sufficient for making an accurate diagnosis. to illustrate the potential difficulties we are reporting three patients in whom the diagnosis of amebiasis was considered but ... | 1983 | 6295138 |
| entamoeba histolytica: correlation between virulence and content of proteolytic enzymes. | intact trophozoites of the virulent entamoeba histolytica strain hm-1:imss (hm-1) destroyed a monolayer of baby hamster kidney (bhk) cells at a higher rate and efficiency than trophozoites of the nonvirulent strain hk-9. the destructive effect could be partially attributed to the proteolytic activity of the amoeba, since quantitative differences were found in the enzymatic activity of the two strains tested. crude extracts or secreted enzymes of hm-1 trophozoites digested azocoll, as well as the ... | 1983 | 6295802 |
| [intestinal protozoal infestation in persons with occupational sewage contact]. | intestinal protozoal infestation and antibodies against entamoeba histolytica were determined in 614 employees of the hamburg sewage works and compared with control groups without occupational sewage contact. the protozoal infection rate was significantly higher in sewer-men than in control persons. there was a positive correlation between the duration of exposure to sewage and the frequency of intestinal protozoal infestation. the risk of infection by sewage was higher than by a short journey t ... | 1983 | 6293794 |
| enterohepatic fistula associated with amebic liver abscess. | 1983 | 6298948 | |
| correlation of virulence and collagenolytic activity in entamoeba histolytica. | the levels of collagenolytic activity of strains hm-1:1 mss (hm-1), (hm-1), 200-nih, and hk-9 of entamoeba histolytica were compared. collagen degradation was evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. conditioned media as well as extracts of the highly virulent strain hm-1 effectively degraded native type i collagen. significantly lower activity was found in the analogous fractions of strains 200-nih and hk-9, which are not as virulent. the collagenolytic activity o ... | 1983 | 6299942 |
| isolation and characterization of actin from entamoeba histolytica. | actin has been identified and purified partially from trophozoites of entamoeba histolytica hmi-imss by a procedure that minimizes proteolysis. in cellular extracts, entamoeba actin would copolymerize with muscle actin, but would not bind to dnase i or form microfilaments. fractionation of the extracts by deae-cellulose and sephadex g-150 chromatography yielded a purified actin that would copolymerize with rabbit skeletal muscle actin or polymerize alone into long filaments at 24 degrees c upon ... | 1983 | 6300064 |
| experimental amoebiasis and the development of anti-amoebic compounds. | 1983 | 6300739 | |
| prevalence of entamoeba histolytica in patients with schistosomal colonic polyposis. | two hundred and fifty-seven egyptian patients were classified into three groups: patients with schistosomal colonic polyposis, those with simple schistosomiasis without polyposis, and a non-schistosomal group. a diagnosis of schistosomiasis was made by clinical history and examination plus three fresh stool examinations or a rectal biopsy. the presence of schistosomal colonic polyps was established by sigmoidoscopy and biopsy of polyps. stool examinations were made on all individuals, using the ... | 1983 | 6301302 |
| entamoeba histolytica in male homosexuals. | the mobility patterns of four enzymes using cultured trophozoites of entamoeba histolytica were examined by electrophoresis. by this means the stocks of amoebae isolated from male homosexuals were characterised into zymodemes. no amoebic stock isolated corresponded to a pathogenic zymodeme. | 1983 | 6303491 |
| entamoeba invadens and e. histolytica: separation and purification of precysts and cysts by centrifugation on discontinuous density gradients of percoll. | the different cell forms in the life cycle of entamoeba invadens (trophozoites, precysts, and cysts) were rapidly and quantitatively separated on density step gradients of polyvinylpyrolidone-coated colloidal silica particles (percoll). with this method, the gradual process of encystation by e. invadens trophozoites could be monitored. percoll gradients were also efficient in separating trophozoites of entamoeba histolytica and bacteria. after purification on percoll, trophozoites display no evi ... | 1983 | 6303827 |
| adherence and ingestion of escherichia coli serotype 055 by trophozoites of entamoeba histolytica. | carbohydrate-binding activity present on the entamoeba histolytica cell surfaces was found to mediate the adherence of two types of bacteria, escherichia coli serotype 055 and salmonella greenside 050. adherence was inhibited by low-molecular-weight carbohydrates (10 mg/ml) such as galactose, lactose, and n-acetylgalactosamine, as well as by asialofetuin and the lipopolysaccharide extracted from e. coli 055. mild periodate oxidation of the bacteria inhibited their adherence, whereas heat inactiv ... | 1983 | 6303959 |
| degradation of biogenic oligosaccharides by beta-n-acetyl-glucosaminidase secreted by entamoeba histolytica. | beta-n-acetylglucosaminidase secreted by entamoeba histolytica was extracted from the growth medium by affinity chromatography on ch-sepharose 4 b coupled to p-aminophenyl-1-thio-beta-2-acetamido-2-deoxyglucopyranoside. the enzyme was further purified by isoelectric focusing, by sequential chromatography on deae-cellulose and sephadex g-150, and by preparative disc gel electrophoresis. chitobiose (betaglcnac1-4glcnac) derived from chitin as well as the oligosaccharides betaglcnac1-4 betaglcua1-3 ... | 1983 | 6304511 |
| separation and characterization of two utp-utilizing hexose phosphate uridylyltransferases from entamoeba histolytica. | two utp-utilizing uridylyltransferases which react with both glucose 1-phosphate and galactose 1-phosphate were isolated from cell-free extracts of entamoeba histolytica. the more specific of these enzymes, glucose-1-phosphate uridylyltransferase, acts preferentially on glucose 1-phosphate, having a maximum velocity 20-fold greater with this substrate than with galactose 1-phosphate. it was purified 200 fold with a 25% yield and has a molecular weight of 45 000. this enzyme requires a reducing a ... | 1983 | 6304512 |
| progesterone-induced amoebic liver abscess in guinea-pigs--a new model. | an experimental model of hepatic amoebiasis in guinea-pigs, simulating the natural infection in man, induced by progesterone following intracaecal inoculation of entamoeba histolytica has been developed. on day 3 after infection, 60% of the animals developed amoebic liver abscess, but on days 7, 11, 15, 20 and 35 after infection liver involvement was recorded in all the animals. no animal in the control group developed hepatic amoebiasis. severity and frequency of caecal lesions was greater in t ... | 1983 | 6304950 |
| safety and efficacy of diphetarsone in the treatment of amoebiasis, non-pathogenic amoebiasis and trichuriasis. | eighty-nine patients infected with entamoeba histolytica, non-pathogenic amoebae or trichuris trichiura were studied prospectively to determine the safety and efficacy of diphetarsone therapy. an additional 75 patients were studied retrospectively to assess further the efficacy of diphetarsone in the treatment of e. histolytica cyst passers. side effects were noted in 9% and included gastrointestinal upset, lightheadedness and headache. transient liver function abnormalities were recorded in 5.6 ... | 1983 | 6304952 |
| immunosuppressive effect of entamoeba histolytica extract on hamsters. | the immune response to sheep red blood cells (srbc) in mice and hamsters injected with an extract of entamoeba histolytica was studied. both the primary and secondary immune response, measured by anti-srbc antibody titers, were unaltered in the mouse, while a significant depression of the primary, but not the secondary, response was observed in the hamster. the effect was greatest when the amebic extract (ae) and srbc were injected on the same day. the number of anti-srbc rosettes formed in the ... | 1983 | 6305049 |
| induced division synchrony in entamoeba histolytica. effects of hydroxyurea and serum deprivation. | synchronous growth of axenic entamoeba histolytica was induced by nutritional deprivation and/or hydroxyurea. axenic amebae maintained in 2% serum culture medium for 17-25 hours and returned to complete tp-s-1 (10% serum) medium showed a single synchronous cycle with a mean division time of 8.8 hours and a synchrony index of 0.47. exposure to 10 mm hydroxyurea for 20-26 hours induced a single synchronous cycle with a division time of 3.0 hours and a synchrony index of 0.85. hydroxyurea was toxic ... | 1983 | 6305219 |
| antigenicity of entamoeba histolytica strain nih 200: a survey of clinically relevant antigenic components. | the molecular weights of the major polypeptides of entamoeba histolytica nih 200 soluble whole extract, and its five fractions eluted from a sephadex g-200 column, were in the range 150,000-9,000 daltons according to sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). autoradiography of sds-page analysis of 125i surface-labeled amebae revealed more than 12 bands, eight of which corresponded to those recovered in soluble extract. considering the pronounced phagocytosis and the ... | 1983 | 6305220 |
| use of a partially purified fasciola gigantica worm antigen in the serological diagnosis of human fascioliasis in egypt. | crude extracts of fasciola gigantica adult worms, when used as an antigen in indirect hemagglutination (iha) and counterimmunoelectrophoresis (ciep) tests, detected all independently diagnosed human f. gigantica and f. hepatica infections but cross-reacted with sera of patients with schistosomiasis and amebiasis. fractionation of this crude worm extract using sephadex g-200 chromatography demonstrated four major protein peaks. antigen from the crest and descending portion of peak ii (mol. wt. ap ... | 1983 | 6305221 |
| antibodies against entamoeba histolytica in human milk and serum in kenya. | a modified enzyme-linked immunosorbent assay (elisa) technique has been developed for the detection of secretory immunoglobulin a (siga) antibodies against entamoeba histolytica. by substituting phosphate-buffered saline-triton for phosphate-buffered saline-tween as the antibody incubating buffer, unspecific absorption of siga was avoided. as revealed by chessboard titrations, the optimal amount of antigen for coating was higher in siga than in igg elisa. a purified antigen from the membrane pel ... | 1983 | 6306046 |
| resistance to lysis by human serum of pathogenic entamoeba histolytica. | a comparison was made of susceptibility to lysis by human sera among five non-pathogenic and 11 pathogenic strains of entamoeba histolytica already characterized into zymodemes. the nonpathogenic strains were found to be uniformly susceptible to lysis. nine of 11 pathogenic strains, including five strains isolated from liver abscesses, were found to be resistant to lysis by serum under identical conditions. resistance to complement-mediated lysis may be an inherent property of most pathogenic st ... | 1983 | 6306876 |
| the use of infectivity titrations for measurement of the viability of entamoeba histolytica after cryopreservation. | an infectivity titration technique is described for the measurement of the viability of entamoeba histolytica. using this method an optimal cryopreservation technique has been developed. when amoebae from young cultures were equilibrated with 7.5% dimethylsulphoxide for 15 minutes at 37 degrees c, cooled to -100 degrees c at 1 degree c per minute, and stored in liquid nitrogen, 1 to 2% regularly survived after thawing rapidly at 37 degrees c and, on occasions, 10 to 12% survived. | 1983 | 6306877 |
| inter-relationship between faecal ph and susceptibility to entamoeba histolytica infection in rats. | 1983 | 6306878 | |
| value of stool examination in patients with diarrhoea. | findings of stool examinations in 1593 patients with diarrhoea due to a single enteric pathogen--enterotoxigenic escherichia coli rotavirus, shigella, campylobacter jejuni, vibrio cholerae 0:1, entamoeba histolytica, or giardia lamblia--were reviewed to determine how well they predicted the agent associated with the diarrhoea. specimens were examined visually for blood and mucus, tested for ph, and examined under a microscope for the presence of red and white blood cells, parasites, and stool fa ... | 1983 | 6307460 |
| polyacrylamide gel electrophoresis of isoenzymes from entamoeba species. | in this preliminary report, we describe a polyacrylamide gel electrophoresis technique for the resolution of isoenzyme patterns of four isolates of entamoeba histolytica and one isolate of entamoeba coli. our findings were similar to previous findings for three enzyme systems: maleic enzyme (malate dehydrogenase [ec 1.1.1.40]), hexokinase (ec 2.7.1.1), and phosphoglucomutase (ec 2.7.5.1). we found preliminary evidence that glucosephosphate isomerase (ec 5.3.1.9) may also differentiate invasive a ... | 1983 | 6308037 |
| the polymicrobial origin of intestinal infections in homosexual men. | to determine the microbial cause and the clinical and pathologic correlates of anorectal and intestinal symptoms in homosexually active men, we performed comprehensive microbiologic studies, anoscopy, sigmoid-oscopy, and rectal biopsy in men examined in a clinic for sexually transmitted diseases. enteric pathogens were found in 95 of 119 consecutive homosexual men with anorectal or intestinal symptoms and in 29 of 75 randomly selected homosexual men without such symptoms (p less than 0.001). the ... | 1983 | 6308444 |
| amoebiasis in a non-endemic country. epidemiology, presenting symptoms and diagnostic methods. | 392 patients with amoebiasis, diagnosed at roslagstull hospital, stockholm during 10 yr, are reviewed. the disease is increasing in frequency, due both to increased travelling by swedish citizens and immigration from non-european countries. the risk for an ordinary charter tourist is, however, rather low. two-thirds of the patients were symptomatic and one-third were regarded as asymptomatic cyst carriers. the importance of repeated examination of stool samples and examinations using different t ... | 1983 | 6308756 |
| entamoeba histolytica causes intestinal secretion: role of serotonin. | lysates of the protozoan parasite entamoeba histolytica altered active electrolyte transport when present on the serosal surface of rabbit ileum and rat colon. the lysate-induced effects on electrolyte transport were similar to those caused by serotonin, and were blocked by bufotenine, an analog known to inhibit the action of serotonin. the transport effects were partially inhibited by antibody to serotonin. the amebic lysates were shown to contain serotonin by radioimmunoassay, high-performance ... | 1983 | 6308760 |
| development and persistence of antibodies to entamoeba histolytica in patients with amebic liver abscess. analysis of 216 cases. | the antibody patterns--obtained by enzyme immunoassay (eia), complement fixation (cf), indirect hemagglutination (iha), and latex agglutination (la)--of 216 patients with amebic liver abscess (ala) were related to the time of onset of illness and to epidemiological data. the usual period between the stay in an entamoeba histolytica-endemic area and clinical manifestation of the disease was 8-20 weeks. increasing antibody concentration affected the sensitivity rates for cf and la in the first 3 w ... | 1983 | 6309024 |
| migration of entamoeba histolytica under agarose. | a procedure for visualizing and quantifying motility of entamoeba histolytica by migration under agarose is described. agarose suspended in tissue culture medium 199 supplemented with bovine albumin was poured into plastic dishes and allowed to harden. six pairs of wells were cut out in a circular configuration. to the inner wells a suspension of e. histolytica in eagle's medium (24 x 10(6) cells/ml) was added, and to the outer wells a chemoattractant or the control medium. after overnight incub ... | 1983 | 6309025 |
| filter paper technique in serodiagnosis of amoebiasis. | 1983 | 6328707 | |
| intestinal protozoan infection in a semicommunal group. | a survey was conducted to determine the prevalence of protozoan parasites in a large semicommunal group in los angeles. protozoan parasites were observed in 151 (69%) of the 220 group members in the study. parasites were observed in stool specimens from 105 (76%) of the 138 children and 46 (56%) of the 82 adults. dientamoeba fragilis was observed in 115 (52%), giardia lamblia in 50 (23%), entamoeba histolytica in 9 (4%), and commensals in 61 (28%). parasitic infection was infrequent in infants l ... | 1983 | 6824128 |
| cutaneous amebiasis. | an infant with cutaneous amebiasis of the vulva and amebic liver abscess is described. epidemiologic investigations and serologic studies were crucial in establishing the diagnosis. the vulvar amebic ulcers responded dramatically to metronidazole therapy. cutaneous amebiasis is a rare complication of entamoeba histolytica infection which should be considered in the differential diagnosis of perineovulvar or penile ulcers. cutaneous amebiasis may also occur on the abdominal wall surrounding a dra ... | 1983 | 6835740 |
| parasitic infection among residents of an institution for mentally retarded persons. | data on occurrence of intestinal parasites among resident and entering populations in a south carolina institution for mentally retarded persons were examined. following an eradication effort in 1973 for trichuris trichiura, the prevalence of this parasite remained below 5 percent for the resident population, with the exception of 1974 and 1975, when small numbers of stool examinations occurred. significantly higher proportions of the entering population, compared to the residents, harbored t. t ... | 1983 | 6837648 |
| [parasitic diarrheas]. | the role of parasites as etiologic agents in diarrhoea is emphasized. in countries of western europe, entamoeba histolytica and giardia lamblia are the most prevalent species, although some other 20 protozoans and helminths may lead to diarrhoeic disturbances. parasitology, pathogenesis, clinical signs, diagnosis, and therapy are briefly outlined. the need for competence on the part of the personnel responsible for laboratory diagnosis is emphasized; also, newly developed immunodiagnostic method ... | 1983 | 6852754 |
| unruptured amoebic liver abscess presenting as acute abdomen. | unruptured amoebic liver abscess is usually not regarded as a surgical emergency. at university college hospital, ibadan, in a two-year period from june 1975 to may 1977, six cases of unruptured amoebic liver abscess underwent emergency exploratory laparotomy because they presented as cases of acute abdomen. the initial diagnoses made by senior physicians included perforated duodenal ulcer, intestinal obstruction, cholecystitis and appendicitis. all patients had persistent draining sinuses after ... | 1983 | 6879689 |
| frequency of entamoeba gingivalis in human gingival scrapings. | a survey was made of gingival scrapings stained by the papanicolaou method to assess the occurrence of entamoeba gingivalis, a nonpathogenic-oral amoeba. positive findings were recorded in 59% of 113 dental patients, and 32% of 96 healthy controls. these figures showed no significant changes during the last 20 years when compared with data published in 1960 and 1963. the existence of e. gingivalis and its rare appearance in the sputum should be known to cytologists because of the morphologic res ... | 1983 | 6881102 |
| an ion-channel forming protein produced by entamoeba histolytica. | we have identified a remarkable ion-channel forming material in virulent strains of entamoeba histolytica that may be responsible for many of the symptoms associated with amoebic dysentery. a polypeptide that we refer to as amoebapore is shed into the growth media and is also found within the amoeba in a high speed sedimentable fraction. amoebapore has the distinctive property of spontaneously incorporating into lipid bilayers, liposomes, and cells, leading to progressive and irreversible change ... | 1982 | 6329705 |
| cytologic detection of parasitic disorders. | parasitic diseases are common in developing countries; yet they are infrequently diagnosed in cytologic material. six cases are described where routine cytologic examination identified the parasites and suggested the diagnosis. there were three cases of entamoeba histolytica infection of the female genital tract and one each of hydatid cyst of the brain, strongyloides stercoralis infection of the lung and filarial infection of the urinary bladder. to our knowledge, cytologic diagnosis of hydatid ... | 1982 | 7048830 |
| vibrio parahaemolyticus: a major cause of travelers' diarrhea in bangkok. | travelers' diarrhea in bangkok, thailand was investigated in a group of 146 hotel guests who had diarrhea during their stay in the city. the identified pathogenic organisms were vibrio parahaemolyticus in 45 (31%), vibrio cholerae in 1, nonagglutinable vibrio in 1, and entamoeba histolytica in 3. identification of enterotoxigenic escherichia coli was not attempted. the study suggests that vibrio parahaemolyticus is a major cause of travelers' diarrhea in bangkok. | 1982 | 7058975 |
| amebic abscess of the liver: surgical aspects. | during the past five years, ten documented cases of hepatic amebic abscess have required surgical intervention at olive view hospital, van nuys, california. three of the patients underwent laparotomy when an hepatic abscess ruptured into the peritoneal cavity, and two required surgical intervention when an abscess invaded adjacent organs. another abscess continued to enlarge despite metronidazole therapy and still another became superinfected with bacteria. in the remaining three patients, surgi ... | 1982 | 7064468 |
| [diarrhea caused by parasites]. | the role of parasites as etiologic agents in diarrhea is emphasized. in countries of western europe, entamoeba histolytica and giardia lamblia are the most prevalent species, although some other 20 protozoans and helminths may lead to diarrheic disturbances. parasitology, pathogenesis, clinical signs, diagnosis and therapy are briefly outlined. the need for competence on the part of the personnel responsible for laboratory diagnosis is emphasized; also, newly developed immunodiagnostic methods m ... | 1982 | 7079687 |
| intestinal parasites in asymptomatic adult southeast asian immigrants. | all previous studies of intestinal parasites in southeast asian refugees have included immigrants with and without symptoms. in order to determine the prevalence of intestinal parasites in asymptomatic southeast asians, 226 adult refugees were studied who had no symptoms or signs suggestive of intestinal parasitism and who submitted one to three stools for examination. fifty-four percent of these asymptomatic adults were infected with one or more potential pathogens, 22 percent with ascaris, 20 ... | 1982 | 7108458 |
| attempts to immunize golden hamsters against entamoeba histolytica. | 1982 | 7125761 | |
| [parasitic infestation and haemoglobinopathies in a population of 1170 refugees from south east asia]. | one thousand one hundred and seventy refugees from cambodia, laos and vietnam were examined in the north of france from 1976 to 1980. 58% of them carried digestive parasites, 22% had anti-plasmodium antibodies and 2% showed circulating plasmodium. the most frequent parasites were hookworms (28%), opisthorchiidae (15%) and giardia intestinalis (12%). strongyloides stercoralis (5,7%), entamoeba histolytica (4,5%), tapeworms (3,3%), human coccidia (1,3%) and trichostrongylus sp. (1%) were less freq ... | 1982 | 7145655 |
| a seroepidemiological study of amebiasis in children of low socioeconomic level in maracaibo, venezuela. | sera collected from 606 children of low socioeconomic level attending the university and children's hospitals in maracaibo, venezuela, were screened for serologic evidence of entamoeba histolytica infection, employing the indirect hemagglutination test. the seropositivity rate was 7.7%. most of the positive sera showed low titers (1:128-1:256). the overall prevalence of positive reactors increased with age. stool examinations done on 516 children revealed cysts or trophozoites of e. histolytica ... | 1982 | 7149096 |
| [use of ultrasound, ultrasound-directed fine needle puncture and aspiration in amebic abscess of the liver. case report and short overview]. | case report on a 48-year-old swiss male living in burundi who suddenly fell ill with vomiting and anorexia, followed by pappy stools, weakness and impotence. he presented with hepatomegaly, while sedimentation rate and alkaline phosphatase were both elevated. stool examination revealed cysts and vegetative forms of entamoeba histolytica, while amebic serology was negative. treatment with ornidazole and later with diloxanide furoate was given. sonography showed two cystoid areas in the right lobe ... | 1982 | 7156963 |
| seroepidemiology of amoebiasis in the villagers in phichit province and urban slum dwellers in bangkok, thailand. | a serological survey was conducted in the villages of phichit province, northern region and in the urban slum communities in bangkok to determine whether amoebiasis was endemic in the areas and to determine the prevalence rates. six rural villages, ethnically and culturally alike with a population of 3,019 and two urban slums with a population of 1,510 were surveyed. sera were tested for indirect hemagglutination antibody (iha) to entamoeba histolytica and the stools examined for the parasite by ... | 1982 | 7170636 |
| altered lipid profile in liver amoebiasis and its emendation with metronidazole treatment. | liver amoebic abscess was produced by introducing 16,000 trophozoites of entamoeba histolytica directly into the livers of growing hamsters. a group of the infected animals received orally 64 mg metronidazole/kg body weight for 5 days from the day of the infection. the treated group was autopsied 7 or 14 days after the treatment. histologically, liquefaction of large parenchymal areas and biochemically, elevation in cholesterol, triglycerides, bile acids and decrease in phospholipids were observ ... | 1982 | 7174072 |
| amebic perforation of the colon. | acute necrotising colitis and perforation of the colon due to amebic infection is usually fatal. only three patients in the present series of nine survived surgery. preoperative diagnosis was not possible and because of an extremely friable, blotting paper like colon, this condition was only suspected at laparotomy. examination of ulcer scrapings for the presence of entamoeba histolytica or histopathology is confirmatory. resection anastomosis is mandatory as less extensive local procedures or b ... | 1982 | 7183622 |
| an epidemiological investigation of health risks related to solid waste salvage and recycling in an egyptian community. | this is the first report of an epidemiological cross-sectional study of solid waste scavengers, called zabaline, in egypt. the zabaline communities provide an important solid waste collection service throughout egypt's urban sector. their economic viability depends entirely on salvaging solid waste for recycling. intestinal parasites were common among the zabaline examined; 48% were found with one or more protozoan or helminth infections. either schistosoma haematobium or s. mansoni or both infe ... | 1982 | 6983749 |
| studies on entamoeba histolytica infection in rural localities in banat. | 1982 | 6303273 | |
| characterization of a membrane pore-forming protein from entamoeba histolytica. | we describe the partial purification and characterization of a pore-forming material (pem) from entamoeba histolytica. the formation of ion channels by pfm was examined in three systems. (a) pfm depolarizes j774 macrophages and mouse spleen lymphocytes as measured by [3h]tpp+ uptake. (b) pfm induces rapid monovalent cation flux across the membrane of phosphatidylcholine-cholesterol vesicles. (c) pfm confers a voltage-dependent conductance to artificial planar bilayers, which is resolved as a sum ... | 1982 | 6294211 |