Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| urinary tract infection with entamoeba histolytica after ileocaecal bladder replacement. | 1985 | 2859507 | |
| isolation and characterization of entamoeba histolytica mutants resistant to emetine. | isolation of emetine-resistant mutants of entamoeba histolytica is described. spontaneous rate of mutation, obtained from a luria-delbrück fluctuation test was 2.5 x 10(-7). the mutagenesis frequency obtained from the number of colonies grown in the presence of emetine, divided by the number of viable trophozoites inoculated in semisolid agar was less than or equal to 10(-7) and it was increased from 10 to 100 fold with ethyl methanesulfonate. two types of clones were isolated: one was cross-res ... | 1985 | 2859522 |
| tolerance of axenically cultured entamoeba histolytica and giardia lamblia to a variety of antimicrobial agents. | twelve antimicrobial agents were tested for use in axenic cultures of entamoeba histolytica and giardia lamblia. ten did not inhibit parasite growth at concentrations of at least two-fold above the effective antibacterial or antifungal levels. we have found the combination of piperacillin (500 micrograms/ml) plus amikacin (125 micrograms/ml) to be useful in axenization of a new strain of e. histolytica, eradication of multiply drug-resistant contaminants in cultures and prevention of bacterial g ... | 1985 | 2859675 |
| zymodemes expressing possible genetic exchange in entamoeba histolytica. | entamoeba histolytica was first seen by lösch (1875), and subsequently described by schaudinn (1903). since that time this organism has only been known to divide by binary fision. observations at molecular level now reveal possible genetic exchange in this species of amoeba. | 1985 | 2859677 |
| effects of cytochalasin b on na+ content and cell volume of entamoeba histolytica. | cells of entamoeba histolytica accumulated k+ and extruded na+ compared to the concentrations of those ions present in the growth medium. pinocytic activity, measured by the uptake of horseradish peroxidase of 125i-polyvinylpyrrolidone, was high (up to 0.3 ml/ml cells per h). upon addition of cytochalasin b, at a concentration (20 microm) that completely blocked pinocytosis, cells lost up to 40% of their na+ content within 90 min; k+ content was not affected or increased slightly compared to con ... | 1985 | 2859891 |
| entamoeba histolytica: purification of cathepsin b. | a cytotoxic cysteine proteinase with a molecular weight of 16,000 was isolated from axenically grown trophozoites of entamoeba histolytica. the enzyme was purified from frozen-thawed strain hm-1 by ion-exchange chromatography on deae-cellulose, organomercurial agarose affinity chromatography, and size-exclusion chromatography. the purified enzyme had proteinase activity that could be demonstrated on azocasein (ph 5), hemoglobin (ph 5), or carbobenzoxy-l-arginyl--l-arginyl-7-amino-4-trifluorometh ... | 1985 | 2860002 |
| vaccination of rabbits against entamoeba histolytica with aqueous suspensions of trehalose-dimycolate as the adjuvant. | rabbits were immunized with soluble entamoeba histolytica antigen with an aqueous suspension of trehalose-6,6'-dimycolate used as the adjuvant. induction of protective immunity in the immunized animals was demonstrated by enhanced humoral and cell-mediated immune responses and 100% survival after challenge. administration of soluble antigen only failed to induce a similar degree of protective immunity. trehalose-6,6'-dimycolate alone produced only a slight increase in nonspecific resistance. | 1985 | 2860068 |
| complement activation by antigenic fractions of entamoeba histolytica. | complement (c) activation induced by entamoeba histolytica in normal non-immune human serum was studied by testing in vitro the ability of different fractions of the trophozoites to cause c3 breakdown. whole trophozoites were found to activate both alternative and classical c pathways. the antibody-independent classical pathway (mgegta inhibitable) c activating capacity was found to greatly increase after disruption of the cell membrane by sonication. subsequent analysis after differential centr ... | 1985 | 2860628 |
| [invasive amebiasis (i): etiology, pathogenesis, epidemiology, and transmission. intestinal amebiasis and its complications]. | 1985 | 2860702 | |
| [invasive amebiasis (ii): extra-intestinal forms and complications. parasitological and serological diagnosis. treatment]. | 1985 | 2860704 | |
| [use of modified asami medium as a monoxenic culture medium for entamoeba histolytica]. | this is a report about the monoxenic cultivation of entamoeba histolytica in asami culture medium. the culture amebae serve as material for antigen preparation, for intrahepatic infections in golden hamsters and also for microscopical observation, s. figs. 2 and 3. | 1985 | 2860761 |
| cytologic demonstration of entamoeba histolytica using immunoperoxidase techniques. report of two cases. | an indirect immunoperoxidase technique to detect entamoeba histolytica in cell samples from patients suspected to have amebiasis is described. using a rat antiserum specific for e. histolytica, the organism was clearly identified both in smears and in cell blocks. this immunoperoxidase technique seems to offer great possibilities for a specific, accurate and rapid identification of amebic infestation in diagnostic cytology. | 1985 | 2860765 |
| use of a monoclonal antibody in an enzyme immunoassay for the detection of entamoeba histolytica in fecal specimens. | an indirect enzyme-linked immunosorbent assay (elisa) was developed for the detection of entamoeba histolytica in human feces, using both a monoclonal antibody and rabbit antisera. it detected from less than 1 to 57 trophozoites of 6 e. histolytica strains. stool specimens were positive by elisa in 18 of 22 (82%) patients with e. histolytica and in 3 of 186 (2%) of patients without demonstrable e. histolytica in their stools. the latter included one from a child living near an asymptomatic cyst ... | 1985 | 2860814 |
| secretory hormones of entamoeba histolytica. | watery diarrhoea as distinct from dysentery is occasionally seen in intestinal amoebiasis, suggesting a component of intestinal secretion. to study the pathogenesis of this watery diarrhoea, we evaluated the effect of lysates of entamoeba histolytica on active intestinal electrolyte transport using rabbit ileum and rat colon studied by the ussing chamber-voltage clamp technique. amoebic lysates added to the ileal and colonic mucosal surfaces did not alter electrolyte transport; in contrast, addi ... | 1985 | 2861068 |
| human amoebiasis: the interaction of lymphocyte surface-bound immune complexes and pmns impairs t cell proliferative responses to e. histolytica mitogen. | in some of the sera from patients with amoebiasis circulating immune complexes are present which are thought to interact with lymphoid cells, enabling them to elicit a burst of oxygen consumption in pmns. the intensity of chemiluminescence is related to the presence of c3+ and fc igg+ cells in the lymphoid cell suspensions employed. the generation and release of highly reactive oxygen derivatives from pmns impair t lymphocyte proliferative responses to the e. histolytica mitogen. the authors sug ... | 1985 | 2861159 |
| enzyme linked immunosorbent assay for serodiagnosis of amoebiasis in children. | 1985 | 2861160 | |
| chemotaxis by entamoeba histolytica. | a micropore membrane procedure to assay taxis by entamoeba histolytica is described and the results of studies of responses to a variety of soluble substances, bacteria, an rat colon washings using this procedure are reported. trophozoites migrated in blind well chambers through 8-micron pore size polycarbonate membranes but not nitrocellulose membranes up to 12 micron pore size. amoebae were attracted toward fresh axenic culture medium (tyi-s), an enzymatic hydrolysate of casein (trypticase), a ... | 1985 | 2861283 |
| incorporation and toxicity of 32p-orthophosphate and occurrence of polyphosphate in entamoeba trophozoites. | we explored the requirements of inorganic phosphate (pi), the incorporation of 32p-orthophosphate (32pi), and the occurrence of inorganic polyphosphate (polyp) in axenic entamoeba cultures. maximal population densities and growth rates of entamoeba histolytica trophozoites were attained in complete tp-s-1 medium. as 32pi concentration was increased in the medium, its own incorporation and the culture growth rate were progressively inhibited, especially in pi-deficient medium. polyp grains were f ... | 1985 | 2861284 |
| amebic colitis. preventing morbidity and mortality from fulminant disease. | uncomplicated amebic colitis is readily treated and has a mortality rate of less than 0.5%. complications necessitating surgical intervention develop in only 6% to 11% of patients with symptomatic disease. however, the mortality rate in these patients ranges from 40% to 100% and stems in part from delays in diagnosis and treatment. patients with known amebic colitis who show signs of systemic toxicity or of localized or generalized peritonitis are at high risk for complications; surgical consult ... | 1985 | 2861601 |
| infectious diarrhea. pathogenesis and risk factors. | our understanding of the pathogenesis of infectious, especially bacterial, diarrhea has increased dramatically. new etiologic agents, mechanisms, and diseases have become known. for example, escherichia coli serogroup o157 is now known to cause acute hemorrhagic colitis. also, e. coli serogroups that produce shiga toxin are recognized as etiologic agents in the hemolytic-uremic syndrome. the production of bacterial diarrhea has two major facets, bacterial-mucosal interaction and the induction of ... | 1985 | 2861742 |
| [anti-amebic effect of polyenic antibiotics]. | all-union research technological institute of antibiotics and medical enzymes, leningrad. institute of epidemiology, virology and medical parasitology, ministry of health of the armenian ssr. the effect of polyenic antibiotics made in the ussr on development of e. histolytica and e. moshkovski was studied. the following antibiotics were used: levorin and its derivatives, mycoheptin, amphotericin b, amphoglucamine and nystatin. the antibiotics were compared with emetine and metronidazole. some dr ... | 1985 | 2861787 |
| interactions between trophozoites of entamoeba histolytica and cells of the immune system. | the interaction between entamoeba histolytica trophozoites and cells of the immune system was studied in vitro. both immune as well as non-immune mononuclear cells (mnc) to some extent were capable of exerting a cytotoxic effect on e. histolytica trophozoites. incorporation of immune serum directed against the trophozoites in the reaction mixture resulted in a marked increase in the killing capacity of mnc. highest killing was observed (96.7 +/- 1.04%) with mnc obtained from animals immunized wi ... | 1985 | 2861805 |
| improved method of producing amoebic liver abscesses in hamsters for screening of systemically active amoebicides. | an improved method of producing hepatic amoebiasis in hamsters has been developed. a serially liver-passaged strain of entamoeba histolytica from experimentally-infected hamsters was used for infection. small pieces from the infected liver were examined for the presence of amoebae and contained approximately 20,000 active trophozoites per piece. such liver pieces, when used as an inoculum, produced infection in all of the animals. the method has been successfully employed for screening of known ... | 1985 | 2861806 |
| [frequency of enteropathogenic micro-organisms isolated in children with and without acute diarrhea]. | 1985 | 2861829 | |
| entamoeba histolytica: impedance measurements and cytotoxicity in the presence of bepridil, verapamil, and cytochalasin d. | entamoeba histolytica, and invasive enteric protozoa, kills mammalian target cells by sequential adherence and cytolytic events. using platinum plate electrodes with an alternating current source placed in a wheatstone bridge circuit, the impedance (resistance to ion flow) of a cell suspension of axenic amebae (strain hm1-imss) was measured. the impedance of the amebic cell suspension, expressed as resistivity (in ohm-cm), was significantly greater than the test solution and increased with decre ... | 1985 | 2862058 |
| differential lectin agglutinability of virulent and avirulent entamoeba histolytica strains. | 1985 | 2862104 | |
| electrophoretic isoenzymes of isolates of entamoeba histolytica before & after revival of their virulence. | 1985 | 2862106 | |
| rapid polymerization of entamoeba histolytica actin induced by interaction with target cells. | within 5 s of challenge of entamoeba histolytica trophozoites with red blood cells (rbc), attachment and deformation of target cells occurred at multiple sites on the amoeba surface. many trophozoite-target interfaces were outlined with a ring of polymerized amoeba actin, revealed by rhodamine-phalloidin staining of glutaraldehyde-fixed and triton-x 100-extracted cells. the beginnings of phagocytic pseudopods rimmed many targets. the phagocytic membrane and underlying actin network grew uniforml ... | 1985 | 2862217 |
| attempts to infect germfree nu/nu mice with entamoeba histolytica. | 1985 | 2862642 | |
| a seroepidemiological survey of amoebiasis in lucknow. | 1985 | 2862724 | |
| intestinal invasive amebiasis: an experimental model in rodents using axenic or monoxenic strains of entamoeba histolytica. | a new experimental model for the production of early stages of invasive intestinal amebiasis in hamsters or guinea pigs with axenic or monoxenic cultures of entamoeba histolytica of the hm1:imss strain is reported. the model is called the washed-closed cecal loop, because it involves the washing out of the cecum contents of conventionally raised animals and the formation of a closed loop, into which amebas are inoculated. colonization occurred in all inoculated animals. after 48 hr, macroscopic ... | 1985 | 2862801 |
| [etiology of acute infantile gastroenteritis in gabon]. | rotaviruses are the main etiology of acute diarrhoeas in gabonese children (11 to 30% according to age). salmonellae (11.4%), shigellae (7.1%) and e. histolytica (7.1%), isolated or associated with enterobacteria, e. coli (3%), giardia and strongyloides stercoralis (1.4%), yersinia enterocolitica (1%) and balantidium coli (0.5%) were also found, without cholera. | 1985 | 2863005 |
| [endocytosis of liposomes by entamoeba histolytica and transport of encapsulated molecules toward the cytoplasm]. | we have examined the interaction of a highly phagocytosing cell: entamoeba histolytica with liposomes of different lipid compositions, and followed, by a semi-quantitative method, the intracellular fate of the entrapped molecules. liposomes containing a small molecule, 6-carboxyfluorescein, are first phagocytosed. then the encapsulated compound migrates from the vacuoles to the cytoplasm. liposomes containing macromolecular substances, such as fluorescent albumin or ferritin, are also phagocytos ... | 1985 | 2863146 |
| in vitro and in vivo interaction between trophozoites of entamoeba histolytica and gerbil lymphoid cells. | the in vitro and in vivo antiamoebic cytotoxic effects of peritoneal exudate cells and mesenteric lymph node lymphocytes of gerbils with cecal amoebiasis or those immunized with amoebic extract were investigated. a differential effect of the lymphoid cells against trophozoites of nonpathogenic and pathogenic strains of entamoeba histolytica was observed. nonpathogenic amoebae were more susceptible to killing by lymphoid cells than pathogenic amoebae in vitro and in vivo in infected or immunized ... | 1985 | 2863217 |
| interaction of human leukocytes and entamoeba histolytica. killing of virulent amebae by the activated macrophage. | capable effector mechanisms in the human immune response against the cytolytic, protozoan parasite entamoeba histolytica have not been described. to identify a competent human effector cell, we studied the in vitro interactions of normal human polymorphonuclear neutrophils, peripheral blood mononuclear cells (pbmc), monocytes (mc), and mc-derived macrophages with virulent axenic amebae (strain hmi-imss). amebae killed neutrophils, pbmc, mc, and mc-derived macrophages (p less than 0.001), without ... | 1985 | 2863284 |
| phospholipase a enzymes of entamoeba histolytica: description and subcellular localization. | cytolysis of target cells by entamoeba histolytica is a rapid, contact-dependent event that appears to involve calcium and amebic phospholipase a enzymes. there are two phospholipase a enzymes of e. histolytica: one calcium independent and optimally active at an acid ph, and a second calcium dependent and most active at an alkaline ph. the amebic calcium-dependent enzyme was inhibited by pharmacological antagonists that have been shown to reduce cytolysis of target cells by intact amebae: phosph ... | 1985 | 2863316 |
| effect of antagonists of calcium and phospholipase a on the cytopathogenicity of entamoeba histolytica. | the in vitro mechanisms by which entamoeba histolytica trophozoites lyse target chinese hamster ovary (cho) cells were examined. calcium chelators ethylenediaminetetraacetate and ethyleneglycol bis (beta-aminoethyl ether)-n,n'-tetraacetate (10 mm) inhibited amebic cytolysis of target cho cells (p less than .01). a putative antagonist of intracellular calcium flux, 8-(n,n-diethylamino)octyl-3,4,5-trimethoxybenzoate (tmb-8; greater than or equal to 250 microm), inhibited amebic adherence and cytol ... | 1985 | 2863317 |
| [native entamoeba histolytica infections]. | 1985 | 2863438 | |
| effect of lanosterol on the susceptibility of mastomys natalensis to entamoeba histolytica infection. | 1985 | 2863883 | |
| entamoeba histolytica: electrophoretic isoenzyme patterns of strains and their virulence in the cecum of gerbils (meriones unguiculatus). | six strains of entamoeba histolytica isolated from humans were characterized by their electrophoretic isoenzyme patterns and by virulence in the cecum of mongolian gerbils (meriones unguiculatus). amebae from symptomatic cases of amebiasis were found to belong to zymodeme group ii, whereas strains isolated from asymptomatic infections belonged to zymodeme groups i or v. strains recently isolated from symptomatic cases of amebiasis were highly invasive in the cecum of gerbils. the most prominent ... | 1985 | 2863988 |
| [immunologic characterization of a monoclonal antibody specific for e. histolytica]. | 1985 | 2864744 | |
| detection of entamoeba histolytica in the pus of amoebic liver abscess by immunofluorescent technique. | 1985 | 2865204 | |
| activation of the alternative and classical complement pathways by entamoeba histolytica. | entamoeba histolytica hm1 supported the activation of human alternative and classical complement pathways in the absence of ameba-reactive antibodies. nonimmune serum depleted of c1q and factor d (nhs s c1q + d) and reconstituted with c1q was able to specifically deposit c3b onto trophozoites and produce lysis. this activity was not modified by the absorption of serum on e. histolytica. serum depleted of factor b allowed c3b binding to amebae. serum devoid of c4 effected only small amounts of c3 ... | 1985 | 2865212 |
| a dot enzyme-linked immunosorbent assay for detection of antibodies against entamoeba histolytica. | a visual micro-dot enzyme-linked immunosorbent assay (elisa) based on detection of antibodies against soluble antigens of axenically grown cultures of entamoeba histolytica is described. the antigen was spotted on a nitrocellulose sheet, the unsaturated sites blocked with bovine serum albumin (bsa) and incubated with 3-fold dilutions of patient sera followed by incubation with protein a conjugated to peroxidase. enzymic activity was evidenced using the substrate 4-chloro-1-naphthol. a positive r ... | 1985 | 2865313 |
| entamoeba histolytica: lymphoreticular changes in gerbils (meriones unguiculatus) with experimentally induced cecal amebiasis. | antibody responses and changes in the lymphoreticular tissues of gerbils with experimental cecal amebiasis were studied from 5 to 60 days pi. changes in the cecum consisted of lymphoid follicle hyperplasia and depletion of lymphocytes, followed by follicle atrophy and histiocytosis. mesenteric lymphadenopathy, and histologic alterations in the lymph nodes paralleled the progressive development of amebic cecal lesions. early in the infection (5 to 10 days pi) mesenteric lymph nodes showed cortica ... | 1985 | 2865345 |
| purine salvage in entamoeba histolytica. | pulse-labeling of the nucleotide pool in entamoeba histolytica with radioactive precursors, and subsequent high performance liquid chromatographic (hplc) analysis of the radiolabeled nucleotides, indicate that e. histolytica is incapable of de novo synthesis of purine nucleotides. hypoxanthine, inosine and xanthine could not be converted to nucleotides in e. histolytica, which suggests the absence of interconversion between adenine nucleotides and guanine nucleotides through formation of imp. ad ... | 1985 | 2865346 |
| experimental infection of rhesus monkeys with entamoeba histolytica mimics human infection. | experimental infection of entamoeba histolytica was successfully established in rhesus monkeys (macaca mulatta). parasites of proven pathogenicity maintained through liver passage in hamsters were used in this study. laparotomized monkeys were inoculated intracecally/intrahepatically with trophozoites, or by oral administration of cysts (2000 cysts/ml). the cysts were isolated from an infected human stool sample. formation of typical amebic lesions in the inoculated tissues along with alteration ... | 1985 | 2865401 |
| [entamoeba histolytica and other intestinal parasites in an institution for the mentally retarded]. | 1985 | 2865836 | |
| entamoeba histolytica, antigens and amoebiasis. | 1985 | 2866068 | |
| inhibition of human monocyte locomotion by products of axenically grown e. histolytica. | the supernatant fluid of axenically grown e. histolytica inhibits chemotaxis, chemokinesis and random mobility of human mononuclear phagocytes (mp) as measured in boyden chambers. human polymorphonuclear phagocytes (pmn) locomotion is apparently unaffected. the factor was found in comparable amounts in the supernatant fluid of axenic cultures of four e. histolytica strains that differed in their human pathogenicity and virulence, as well as in two entamoebas non-pathogenic for man. this dialysab ... | 1985 | 2866481 |
| [culture methods in the diagnosis of intestinal parasitic protozoans in man, particularly entamoeba histolytica]. | 1985 | 2866849 | |
| multilayer-enzyme linked immunosorbent assay (ml-elisa) for detection of entamoeba histolytica trophozoite coproantigen. | multilayer elisa (ml-elisa) method for entamoeba histolytica trophozoite coproantigen was developed for diagnosis of amoebiasis and its usefulness vis-a-vis microscopic examination analysed for paired specimens from 87 patients from lucknow and hyderabad, india. all the stool specimens positive for trophozoites were also found to be positive in ml-elisa. out of a total 32 samples positive for cysts in microscopy 21 (65.62%) gave positive reactions for coproantigen. results on 26 samples drawn fr ... | 1985 | 2867033 |
| which amoebae are harmful? | 1985 | 2867035 | |
| adherence of entamoeba histolytica trophozoites to rat and human colonic mucosa. | we studied the adherence of [3h]thymidine-labeled axenic entamoeba histolytica (strain hm1-imss) to in vitro preparations of rat and human colonic mucosa. studies were performed with fixed or unfixed rat colonic mucosa, unfixed rat mucosa exposed to trypsin, unfixed rat submucosa, and fixed human colonic mucosa. twenty percent of the amebae adhered to fixed rat colonic mucosa; adherence was specifically inhibited by n-acetyl-d-galactosamine (galnac), galactose, and asialofetuin. the adherence of ... | 1985 | 2580787 |
| subcellular fractionation of amoebapore and plasma membrane components of entamoeba histolytica using self-generating percoll gradients. | separation and initial characterization of subcellular organelles from entamoeba histolytica have been achieved by the use of self-generating percoll gradients. adequate resolution was obtained within one hour of amoeba homogenization. the ion-channel forming activity, amoebapore, was found associated with highly dense, small diameter particles, that were resolved from the numerous digestive vacuoles, and the plasma membrane. following iodination of intact trophozoites with lactoperoxidase, the ... | 1985 | 2581133 |
| fluorometric analysis of transferable membrane pores. | when pore-forming factors insert into the hyperpolarized membranes of lipid vesicles, ion gradients are rapidly equilibrated, effecting complete depolarization. this process can be conveniently followed with a potentiometric cyanine dye. the generality of the method is demonstrated by applications to three diverse materials. the well-studied gramicidin channel is used to demonstrate that the method is sensitive down to concentrations of 10(-12)m. an extract from the shark repellent skin secretio ... | 1985 | 2581609 |
| the effect of splenectomy on resistance of mice to entamoeba histolytica infection. | the role of the spleen in amoebic infection was examined in mice, using strains selected as being either genetically-susceptible (c57bl/6) or genetically-resistant (a/j) to amoebiasis. splenectomized and sham-operated animals were inoculated intracaecally with 2.5 x 10(5) polyxenic trophozoites of e. histolytica at 6, 12 and 15 days post-splenectomy. the animals were killed 6 or 12 days after infection and the parasite burden was evaluated. removal of the spleen in both susceptible and resistant ... | 1985 | 2999680 |
| [epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics]. | diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ... | 1985 | 3000920 |
| comparison between the efficacy of a single dose of secnidazole with a 5-day course of tetracycline and clioquinol in the treatment of acute intestinal amoebiasis. | a study was carried out in 80 patients with acute intestinal amoebiasis to compare the efficacy of treatment with a single oral dose of 2 g secnidazole and a 5-day course of 750 mg tetracycline plus 1 g clioquinol per day. patients were allocated at random into one or other treatment group and returned for clinical assessment and microscopic examination of stools for the presence of the trophozoite or haematophagous form of entamoeba histolytica on days 1 to 7, 14, 21 and 28. the results showed ... | 1985 | 2932742 |
| [asymptomatic parasitic infection in infants under 6 months]. | 1985 | 2873630 | |
| failure to establish experimental entamoeba histolytica infection in mongolian gerbils (meriones unguiculatus) naturally infected with entamoeba muris. | 1985 | 2870571 | |
| assessment of validity of counterimmunoelectrophoresis and elisa in the routine diagnosis of amoebiasis. | counterimmunoelectrophoresis (cie) was used to detect antibodies against entamoeba histolytica in 99 sera from amoebiasis suspected patients using antigens from the hk-9 and ht-12 strains of axenically cultivated e. histolytica. there was no significant difference of seropositive rates using antigens from these two strains suggesting the both strains were equally suitable for use in the routine cie test for the diagnosis of amoebiasis. in comparison with elisa, the cie test was a little less sen ... | 1985 | 2869587 |
| differences in adhesion, phagocytosis and virulence of clones from entamoeba histolytica, strain hm1:imss. | 1985 | 2869003 | |
| use of periodate activated sepharose 4b immunoadsorbent in purification of anti entamoeba histolytica antibody. | 1985 | 2870982 | |
| coproantibody study in entamoeba histolytica, entamoeba hartmanni and other parasitic infections. | 1985 | 2870983 | |
| amoebic pericarditis. | 1985 | 2871111 | |
| mitogenic factor for t inducer/helper cells in entamoeba histolytica extracts. | 1985 | 2882454 | |
| the biochemistry and functional morphology of the entamoeba. | 1985 | 2409275 | |
| routine procedures for examination of stool and blood for parasites. | the diagnosis of a parasitic infection depends upon demonstration of the organism in stool, blood, or tissue specimens, and a variety of relatively simple procedures is available for this purpose. in this article, the author describes those procedures that are most frequently used on a routine basis. | 1985 | 2410850 |
| [translation of rna obtained from entamoeba histolytica and its characterization of a specific monoclonal antibody]. | 1985 | 2413554 | |
| histopathological diagnostics of liver amoebiasis in cambodia. | results of histopathological examination of a solitary chronical amoebic abscess in the liver of a patient from cambodia are described. the abscess was at a proliferously reparative phase and entamoeba histolytica was detected at the border between the necrosis and inner wall of abscess, as well as in the proper non-specific granulation tissue of the inner layers of abscess capsule. a differential diagnosis of the amoebic abscess from similar parasitary or pseudoparasitary liver lesions and diff ... | 1985 | 2417921 |
| antibody response of patients harbouring different zymodemes of entamoeba histolytica to homologous and heterologous antigens. | entamoeba histolytica isolates characterized into four zymodemes were used as antigens in the ifat; with 17 sera obtained from patients harbouring different amoebic isoenzyme groups. the method of cultivation used was found to influence the ease of reading the ifat. reactivity of serum samples, obtained from the selected patients (i.e. with varied forms of amoebiasis), against homologous and heterologous antigens did not reveal any antigenic differences among amoebic zymodemes. the present resul ... | 1985 | 2418210 |
| [medical problems in refugees from sri lanka (tamil)]. | recently many refugees from sri lanka have arrived in europe. the purpose of the present investigation was to analyze the subjective complaints and diagnoses in these refugees. one hundred refugees (97 males, 3 females, age 19 to 42 years) were investigated. the most common reasons for consulting a general internist were cough (23%), general pain in soft tissue and joints (21%), disorders of the gastrointestinal tract (19%) and ear or throat complaints (15%). in 43% of the patients no diagnosis ... | 1985 | 3969544 |
| [digestive manifestations of the acquired immunodeficiency syndrome (aids): study in 26 patients]. | we studied the gastrointestinal manifestations in 26 cases of aids. the patients belonged to two different epidemiological groups: the first group included thirteen french homosexual men, the second group included 6 haitians, 6 africans and a pakistanian, none of them admit homosexual activity. the clinical manifestations were: chronic watery diarrhea in 17 cases, bloody diarrhea in 2 cases; loss of weight in the 26 cases; dysphagia in five cases; jaundice in one patient (due to kaposi sarcoma o ... | 1985 | 3996815 |
| cell-mediated immunity in hepatic amoebiasis. | cell-mediated immunity (cmi) was measured by blastic transformation of peripheral blood lymphocytes in 26 patients with amoebic liver abscess (ala) and matched control subjects with no demonstrable clinical amoebiasis. during active disease, the mean mitogenic response, measured by the stimulation index of the patients' lymphocytes to entamoeba histolytica antigen, was increased (mean +/- sd: 25.98 +/- 46.62 compared with 11.27 +/- 21.39), whereas that to phytohaemagglutinin (pha) was reduced (5 ... | 1985 | 4002286 |
| health status of teenage school boys in eastern sudan. | in july, 1981 a health survey of teenage boys was conducted in a medical clinic in the eastern sudan, run by the lalmba association, a non-profit aid organization based in the us. the study population was a group of 231 students ranging in age from 13-18, some of whom board at the school. data consisted of a general health questionnaire, height and weight measurements, and a thorough physical examination including stool sample. average height and weight were both less than western teenagers, ... | 1985 | 4006817 |
| mitogen-like monoclonal anti-actin antibodies. | monoclonal antibodies (igm kappa) have been produced to actin isolated electrophoretically from l cell extracts. these monoclonal anti-actin antibodies bind to intact l cells and modulate dna synthesis and cell proliferation, much like affinity-purified polyclonal rabbit antibody to the same mr 42,000 actin. in addition, monoclonal antibodies specific for actin from entamoeba histolytica also bound to and modulated the growth of l cells. a monoclonal antibody directed against a neuroblastoma sur ... | 1985 | 4008931 |
| histopathology of hepatic amoebiasis in guinea-pigs infected through intracaecal and intramesenteric routes. | the comparative histopathological details of amoebic liver abscess were studied following intracaecal and intramesenteric inoculation of entamoeba histolytica into guinea-pigs. the histological changes of amoebic liver abscess formed via the former route were similar to those seen in man. these consisted of a central necrotic area with few lymphomononuclear cells and e. histolytica trophozoites at the periphery of the lesion. the amoebic liver abscess formed via the intramesenteric route, howeve ... | 1985 | 4035733 |
| a mouse model for entamoeba histolytica infection. | 2 strains of entamoeba histolytica, saw 760 and saw 408 (non-invasive zymodeme ix and invasive zymodeme ii respectively, sargeaunt & williams 1979) were administered intra-intestinally to 15 inbred and 3 outbred strains of mouse. all were known to be free of the mouse amoeba e. muris. during the investigation, all animals were maintained in plastic film isolator units, under gnotobiotic conditions. both zymodemes were found to be capable of infecting all 18 strains of mice for varying periods of ... | 1985 | 4068657 |
| [entamoeba histolytica as the cause of urinary tract infection after cystectomy and internal urinary diversion with an ileocecal bladder]. | 1985 | 4082329 | |
| intestinal parasites in kampuchea, takeo province. | fresh stool samples obtained from 1407 adult patients who sought treatment in takeo province hospital and 332 takeo preschool and school-age children from 3 to 18 years of age were examined for the presence of intestinal parasites using the technique of native preparation and the flotation method of faust with subsequent staining with lugol solution to demonstrate cysts. in hospital patients, a total of 13 protozoan and 9 helminth species were diagnosed. the prevalence of entamoeba histolytica ( ... | 1985 | 4086816 |
| experimental amoebiasis in the guinea-pig: sequential histological abnormalities following intracaecal injection of entamoeba histolytica. | the histological abnormalities of amoebic colitis have been well described but their evolution over a period of time has not been clearly examined. to study this, three- to four-week-old guinea-pigs were inoculated intracaecally with 80,000 to 100,000 entamoeba histolytica trophozoites, left untreated and killed at 3, 5, 8, 11, 14 and 21 days, when the caecum was removed and the tissue sectioned and stained with h & e and pas. although there was considerable variation in the type of histological ... | 1985 | 4095744 |
| [gastrointestinal manifestations of aids]. | we studied 26 cases of digestive manifestation in aids. the 26 patients were divided into two different epidemiological groups: 13 homosexual men, constituted the first group; no homosexual patient was in the second group which included 6 haitians, 6 africans and a pakistanian. the clinical manifestation were: a watery chronical diarrhea in 17 cases a bloody diarrhea in 2 cases; a loss of weight in the 26 cases; a dysphagia in five cases; a jaundice in one patient (due to kaposi sarcoma of the a ... | 1985 | 4096504 |
| chronic diarrhoea in nigerian children. | to study the etiology of chronic childhood diarrhea among nigerian children, 142 patients, aged 6 months to 5 years, with diarrhea for at least 1 month, were evaluated; the study took place during january-december 1983 at the ahmadu bello university teaching hospital, zaria, northern nigeria. enteropathogenic agents were identified in stools of 90 (63%) patients. giardia lamblia and entamoeba histolytica were most commonly detected, representing 41% and 23%, respectively, of all parasitic path ... | 1985 | 3833911 |
| medical problems of the homosexual adolescent. | physicians treating adolescents should take a complete sexual history, including sexual orientation and practices, to determine whether their patients are homosexually active. lesbians are at very low risk for sexually transmitted diseases, but they do have other health concerns. four general groups of conditions may be encountered in homosexually active men: classical sexually transmitted diseases (gonorrhea, infections with chlamydia trachomatis, syphilis, herpes simplex infections, genital wa ... | 1985 | 3839219 |
| squamous cell carcinoma of the uterine cervix subsequent to amebiasis. | we studied the development of a well-differentiated squamous cell carcinoma of the uterine cervix five years after cervical infection with entamoeba histolytica by documenting the findings of specimens obtained by repeated biopsies in a 69-year-old woman. to our knowledge, this is the first report of a malignant neoplasm of the uterine cervix subsequent to amebiasis. amebiasis superimposed on malignant neoplasms of the female genital tract has been reported before. we raise the question of a pos ... | 1985 | 3840988 |
| utility of culture technique for both faeces and biopsy material for the diagnosis of entamoeba histolytica. | 1985 | 3848408 | |
| amoebiasis and its control. | on a global scale, 480 million people were recently estimated to be infected with entamoeba histolytica and some 48 million people suffer from invasive amoebiasis. the latter, in the intestinal and extra-intestinal forms, accounts for 40 000 to 110 000 deaths annually. in view of this considerable mortality and morbidity, more research is urgently needed so that more effective control strategies may be introduced.this update article reviews the magnitude of the problem, clinical and laboratory d ... | 1985 | 3876168 |
| lymphocyte subpopulations transformation studies in an experimental model of intestinal and hepatic amoebiasis. | the lymphocyte subpopulations in peripheral blood and blast transformation were studied on days 0, 3, 7, 11, 15, 20 and 35 after infection in progesterone-treated guinea-pigs which had been experimentally infected with entamoeba histolytica by intracaecal inoculation. in animals with intestinal infection, lowered t cell numbers and blast transformation were seen during the acute phase with a subsequent tendency to recover. in guinea-pigs with hepatic amoebiasis, both these parameters were signif ... | 1985 | 3879396 |
| proteinase activities of entamoeba histolytica cytotoxin. | the proteinase activity of the low molecular weight cytotoxin of entamoeba histolytica was correlated with its cytotoxicity. gel-filtered amebal toxin (mol wt 10-30,000) proteinase activities could be assayed on azocasein at ph 6 or on hemoglobin at ph 4.5. proteinase activity was inhibited by serum fractions, thiol reagents, heavy metals, leupeptin, and antipain. the cytotoxic activity of gel-filtered amebal toxin was inhibited by serum fractions, leupeptin, and antipain. increased proteinase a ... | 1984 | 6327455 |
| [intestinal amebiasis. a diagnosis with many faces]. | 1984 | 6327559 | |
| protozoal enteric infections in homosexual men. | the prevalence of enteric protozoal infections was examined in a group of 200 homosexual men. entamoeba histolytica (e. histolyt .) was found in 22.8%, apathogen amoeba in 54.5% and giardia lamblia (g. lamblia) in 9.1% of the participants. antibodies against e. histolyt . were significantly higher in subjects carrying e. histolyt . than in the group with apathogen amoeba and in uninfected participants. clinical symptoms were not associated with protozoal infection. the analysis of a number of va ... | 1984 | 6328099 |
| concomitant and secondary bacterial infection of the pus in hepatic amoebiasis. | in a series of 48 consecutive patients with clinically diagnosed uncomplicated amoebic liver abscess (ala), the pus obtained by closed drainage of the abscess was bacteriologically sterile. secondary bacterial infection of the ala as a direct result of the aspiration procedure occurred in only 1 patient, the causative organism being escherichia coli. entamoeba histolytica was identified in a total of 39 cases (81%) by means of direct microscopy of the pus as well as culture methods. | 1984 | 6328682 |
| detection of amoebic antibodies using fractionated antigen in tia and iha tests. | thin layer immunoassay (tia) test was used to detect anti-amoebic antibodies in the sera obtained from immunized guinea pigs. a few human sera samples were also used for antibody detection. the tia and iha results with whole amoeba antigen and fractionated antigen preparations were compared. both test procedures gave better results with fractionated antigen. the results in tia were better than in iha tests. thin layer immunoassay technique can be conveniently used as an alternative to iha test f ... | 1984 | 6328711 |
| murine amebiasis: the role of the macrophage in host defense. | the congenitally athymic nude (nu/nu) mouse was studied as a model for amebic liver disease, using entamoeba histolytica. despite intrahepatic inoculation of massive numbers of amebae, we could not produce sustained infections in nu/nu mice. we also failed to induce hepatic amebiasis in thymus-intact nu/+ and +/+ littermates, or in nu/+ mice pretreated with rabbit antimouse thymocyte globulin. humoral response was measured in both the nu/nu and nu/+ mice. nu/+ but not nu/nu animals developed a s ... | 1984 | 6329011 |
| microvolume, kinetic-dependent enzyme-linked immunosorbent assay for amoeba antibodies. | we describe a microvolume enzyme-linked immunosorbent assay based on enzyme rate kinetics. antigens from entamoeba histolytica were adsorbed in wells of disposable polystyrene strips containing 12 flat-bottom wells. after exposure to the serum of a patient and peroxidase-labeled anti-human immunoglobulin g, the rate of color change in specific substrate was determined by eight sequential readings of individual wells over a 2-min period with a microcomputer-controlled model mr-600 automated plate ... | 1984 | 6321547 |
| entamoeba histolytica: a eukaryote without glutathione metabolism. | entamoeba histolytica was found to grow normally without producing glutathione and the main enzymes of glutathione metabolism, indicating that glutathione is not essential for many eukaryotic processes. this parasitic amoeba is an unusual eukaryote whose special features may help define the crucial functions of glutathione in those eukaryotes that do use it. since entamoeba histolytica lacks mitochondria and the usual aerobic respiratory pathways, the finding that it grows without glutathione an ... | 1984 | 6322306 |
| amoebiasis in homosexual men. | the clinical, histopathological, and serological features of 35 homosexual men with infection with entamoeba histolytica were studied and compared with a group of 35 non-infected homosexual men. each isolate was of zymodeme type i. although there was no significant difference in the numbers of infected and non-infected men with gastrointestinal symptoms (48.6% and 28.6% respectively), the mean duration of symptoms was greater in with amoebiasis (p less than 0.05). the histology of the rectal muc ... | 1984 | 6323279 |
| kinetics of peripheral blood t cell numbers and functions in patients with burns. | cell-mediated immunity (cmi) was assessed in 58 patients with burns involving 10% to 40% of the total body surface area (tbsa), and in 16 normal healthy individuals, by using two parameters, the total t-cell counts in peripheral circulation and t-cell function as indicated by the leukocyte migration inhibition (lmi) test. three recall antigens from candida albicans, s. typhi, and e. histolytica were used to elicit the lmi. t-cell function was found to be significantly depressed in burns involvin ... | 1984 | 6323729 |
| [an unnecessarily long course of a disease]. | 1984 | 6324008 |