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epidemiology of antibiotic resistance in human isolates of enterobacteriaceae in sicily.the authors have studied the antimicrobial susceptibility of 1073 clinical isolates of various genera of enterobacteriaceae (collected during the period july-december 1988) to ampicillin, piperacillin, cefotaxime, ceftazidime, ceftriaxone, aztreonam, imipenem, gentamicin, amikacin, netilmicin, norfloxacin, and ciprofloxacin. antimicrobial susceptibility was determined by bauer-kirby disk diffusion method. of 1073 tested bacteria, 704 (65.6%) produced beta-lactamase detectable by nitrocefin test. ...19902332782
[microflora of suppurative inflammatory foci in surgical patients and its sensitivity to antibiotics].materials from 1134 patients with various surgical infections were tested. from the materials collected from purulent inflammatory foci 2137 cultures were isolated. many of them belonged to micrococcaceae (63.7 per cent). cultures belonging to enterobacteriaceae and streptococcaceae were isolated from 50.9 and 25.2 per cent of the patients respectively. isolates belonging to other families were less frequent: diphtheroids were isolated from 13.3 per cent of the patients and pseudomonas spp. were ...19902334244
a medium for the selective isolation of edwardsiella ictaluri.a selective medium, called edwardsiella ictaluri medium (eim), has been formulated for the isolation of edwardsiella ictaluri. the medium inhibits the growth of most gram-negative bacteria, except proteus sp., serratia marcescens and some isolates of aeromonas hydrophila and yersinia ruckeri. the bacteria that grow on the eim are easily differentiated from e. ictaluri based on colony morphology. the eim inhibits gram-positive bacteria with the exception of enterococci. the addition of fungizone ...19902338726
the hpma hemolysin is more common than hlya among proteus isolates.two different hemolysins, hpma and hlya, have been reported in proteus spp. to study the distribution of these hemolysins among proteus strains, isolates from various infections and normal feces were screened for hemolysin production. all 63 proteus mirabilis strains and 23 of the 24 proteus vulgaris strains produced a calcium-independent hemolytic activity detectable in cell-free supernatants. the calcium-independent activity was due to hpma; this activity correlated with the presence of hpma s ...19902341181
cytotoxic activity of the proteus hemolysin hpma.we previously showed that hpma is the hemolysin determinant most commonly found among proteus isolates. to assess the potential contribution of hpma to virulence, we first characterized the toxic activities of this hemolysin. hemolytic activity was present in total cell cultures and cell-free supernatants of proteus clinical isolates as well as escherichia coli containing cloned hpm genes. hpma also possesses cytotoxic activity which was detected by a chromium release assay against a variety of ...19902341182
morganella morganii urease: purification, characterization, and isolation of gene sequences.morganella morganii, a very common cause of catheter-associated bacteriuria, was previously classified with the genus proteus on the basis of urease production. m. morganii constitutively synthesizes a urease distinct from that of other uropathogens. the enzyme, purified 175-fold by passage through deae-sepharose, phenyl-sepharose, mono-q, and superose 6 chromatography resins, was found to have a native molecular size of 590 kilodaltons and was composed of three distinct subunits with apparent m ...19902345135
septicemia in the elderly: incidence, etiology and prognostic factors.a study on septicemia in the elderly (mean age 80.3 +/- 9.1 years) was carried out during 1982-85. a total of 184 episodes of bacteremia occurred in 175 patients (incidence rate 7.2%); 61% were attributed to community-acquired sepsis. gram-negative microorganisms accounted for 64% of all episodes and gram-positive for 30%. the overall mortality was 18.3%. using univariate analysis, significant factors associated with a high mortality were: hospital-acquired sepsis, respiratory infections as sour ...19902347685
[statistical studies on bacteria isolated from urinary tract infections(report 5)].we examined bacteria isolated from urine of patients in our urological department from 1985 through 1987. a total of 2,566 strains were isolated from outpatients. they were 834 strains of gram-positive coccus (32.5%). e. coli (20.8%) was isolated most frequently, followed by proteus sp. (12.6%), enterococcus (9.6%), p. aeruginosa (6.7%), s. epidermidis (5.5%), and k. pneumoniae (3.7%) and others. a total of 784 strains were isolated from inpatients. they were 313 strains of gram-positive coccus ...19902353605
chronic suppurative otitis media in sudanese patients.a retrospective study was carried out to show the pattern of chronic suppurative otitis media in sudanese. patients with chronic suppurative otitis media were 43% of all patients with ear problem. the majority of patients affected by the disease were between 11-30 years with even distribution. males were affected more than females. the duration of symptoms varied between 0-20 years but most of the patients had the disease for over six years. pseudomonas aeruginosa, pseudomonas pyocyaneous and b. ...19902354676
infected wounds and repeated septicemia in a case of factitious illness.during 19 years an assistant nurse, now 35 years old, has been repeatedly treated for several malingered and self-induced disorders escalating to self-mutilation. an ulcer of her right leg never epithelialised in spite of various local treatments and surgical intervention. during repeated attacks of self-induced septicemia altogether 11 different bacterial species were isolated; on 8 occasions rhodococcus equi. the septicemias were successfully treated with antibiotics. the underlying psychiatri ...19902356446
proteus syndrome (report of three cases). 19902357192
proteus syndrome: report of a case with severe brain impairment and fatal course.a patient with proteus syndrome is reported. he had hemihypertrophy, bilateral hypertrophy of the third fingers, skin anomalies, and varicosities, as well as mental retardation, seizures resistant to anticonvulsant treatment, and a very severe course with death at the age of 2 years.19902359106
pericarditis due to salmonella dublin. 19902362900
otogenic brain abscess with pneumocephalus. 19902365472
[pneumonitis as a complication of low-dose methotrexate therapy in chronic polyarthritis].pneumonitis has been reported to be a rare complication of low-dose mtx treatment. this paper describes a 62-year-old male patient in whom clinically successful low-dose treatment was applied. after six months of treatment, a gradually progressive pulmonary symptomatology developed and abruptly deteriorated. radiological examination revealed interstitial and intra-alveolar densifications; functional analysis revealed partial respiratory failure. the histological finding of interstitial lymphocyt ...19902367474
numerical analysis of electrophoretic protein patterns of providencia stuartii strains from urine, wound and other clinical sources.eighty-six strains of providencia stuartii (mainly of human origin) were characterized by one-dimensional sds-page of cellular proteins. the strains came from various countries; 52 were from urine, 11 from wounds, five from blood (one of these also from urine), four from ear infections, two each from faeces and sputum, one from 'alimentation' and nine from unknown sources. the protein patterns, which contained 45 to 50 discrete bands, were highly reproducible. the patterns of 46 prov. stuartii s ...19902370236
renal tubular acidosis and urinary tract infection. 19902370605
[effect of immunization with proteus vaccine on the donor organism]. 19902373348
[quantitative determination of urine bacteria in the diagnosis of urinary tract infections in children].bacteria counts in fresh, unstained, uncentrifuged urine specimens, using a phase-contrast microscope, magnification x 400, and a hemocytometer chamber, proved to be a very useful method not only of excluding (specificity 0.92) but also identifying urinary tract infection (sensitivity 0.90) in children. the presence of greater than 5/0.1 ml bacteria in the urine was taken as the threshold value. the evaluation of leukocyturia alone did not alter the sensitivity of the method, even when it was as ...19902377151
rocky mountain spotted fever in new york state.clinical and laboratory data provided in 214 rocky mountain spotted fever (rmsf) case reports received by the new york state department of health during 1977-1988 were analyzed to determine recent trends of this disease observed infrequently, but continuously, in new york state residents. age, sex, geographic exposure, onset, symptoms, laboratory test, treatment, and hospitalization data are presented. despite a 69% decrease of disease incidence from 1977 (0.368 cases per 10(5) population) to 19 ...19902378453
urea protects helicobacter (campylobacter) pylori from the bactericidal effect of acid.colonization of the stomach with helicobacter (campylobacter) pylori is common in patients with duodenal ulcer disease, which is known for its high acid secretion. although the bacterium is usually isolated by culture of a gastric biopsy specimen, viable organisms may sometimes be found in the acidic gastric juice. it was postulated that urease, by generating ammonia, protected h. pylori from acid. to test this hypothesis, the ph susceptibility of h. pylori, proteus mirabilis, and the urease-neg ...19902379775
bacteremia and subcutaneous abscess caused by proteus penneri in a neutropenic host.proteus penneri bacteremia and concomitant subcutaneous infection developed in a neutropenic patient with acute lymphocytic leukemia. the skin infection occurred while the patient was being treated empirically with cefoperazone and metronidazole. this case demonstrates the invasive potential of this microorganism in the proper setting.19902380386
synchronous caverno-spongious thrombosis and fournier's gangrene.fournier's or genito-perineal gangrene of the male genitalia typically spares the testis, urethra and deep penile components. it usually is polymicrobial and synergistic. to our knowledge, this is the first reported case in the literature with severe, deep, full penile thrombosis that caused a severe urethral stenosis and required later surgery. only proteus mb. could be isolated and gave rise to a disseminated intravascular coagulation syndrome that has probably accentuated the thrombotic penil ...19902383054
direct introduction of a formamido group into the 7 alpha (6 alpha)-position of cephalosporins (penicillins).a novel direct introduction of a formamido group into the 7 alpha (6 alpha)-position of cephalosporins (penicillins) was achieved by treatment of 7 beta (6 beta)-[(3,5-di-tert-butyl-4-oxo-2,5-cyclohexadien-1- ylidene)methylimino]cephem (penam) esters with n,n-bis(trimethylsilyl)formamide, followed by deblocking with girard reagent t to give the corresponding 7 alpha (6 alpha)-formamido-7 beta (6 beta)-amino derivatives. three 7 alpha-formamidocephalosporins were prepared by the conventional n-ac ...19902387776
ambiguous genitalia in the proteus syndrome.an 11-year-old girl was followed up since birth because of hemihypertrophy of the left leg and thigh, multiple pigmented nevi and subcutaneous tumors typical of the proteus syndrome. because of clitoromegaly and scrotal-like hypertrophy of the labia majora, she had an endocrine evaluation the results of which were normal. the findings are thought to represent local genital hypertrophy. ambiguous genitalia have to be added to the phenotype of the proteus syndrome.19902389808
[comparison of bacteriostatic ability of oleum of perilla frutescens (l.) britt., cinnamomum cassia presl and nipagin a].a comparison of bacteriostatic ability has been carried out among oleum of perilla frutescens and cinnamomum cassia, benzoic acid and nipagin a. the result shows that the inhibiting effect of perilla frutescens and cinnamomum cassia on microorganisms inoculated and spontaneously contaminated is similar to or obviously superior to nipagin a and benzoic acid.19902390180
pyodermas: an analysis of 127 cases.in an analysis of 127 patients with pyodermas, 38 (30%) had primary pyodermas and 89 (70%) had secondary pyodermas. seventy-one percent (71%) of the primary pyodermas were due to staphylococcus aureus (s. aureus). five (5%) of the primary pyodermas were due to multiple organisms. among the patients with secondary pyodermas, 45% were due to s. aureus, 21% streptococci, 9% pseudomonas and 9% proteus species. forty-four percent (44%) of the secondary pyodermas were due to multiple organisms. s. aur ...19902393234
the development of a transport and enrichment medium for campylobacter fetus.a transport and enrichment medium was developed for campylobacter fetus. from inocula of between 10 and 35 organisms the medium was able to support the multiplication of 19 of 21 strains of c. fetus if the medium was incubated immediately after inoculation; when incubation was delayed for 3 days after inoculation, only seven of the 21 strains multiplied. from inocula of 100-350 organisms all 21 strains multiplied following immediate incubation, and 20 of 21 when incubation was delayed for 3 days ...19902397372
cephalosporins to carbapenems: 1-oxygenated carbapenems and carbapenams.the photo "wolff" rearrangement of readily available 2-diazoceph-3-em oxides (1) directly affords carbapen-2-ems, allowing a facile entry into a ring system previously accessible only by total synthesis, lengthly semisynthesis or fermentation. the chirality of the cephalosporin is accurately translated into the corresponding carbapenem. the resulting 1-oxocarbapenems (2) were selectively transformed through reduction into 1-oxygenated carbapenems and carbapenams (3 and 4, respectively). on micro ...19902404120
proteus vulgaris empyema and increased pleural fluid ph. 19902404705
6-(substituted methylene)penems, potent broad spectrum inhibitors of bacterial beta-lactamase. ii. racemic furyl and thienyl derivatives.a series of racemic 6-(substituted methylene)penems have been prepared. these compounds contain a 5-membered monoheteroaromatic ring at c-8. the antibacterial/synergistic and beta-lactamase inhibitory activities of both e- and z-isomers and 2-substituted derivatives are compared.19902407709
nucleotide sequencing of the proteus mirabilis calcium-independent hemolysin genes (hpma and hpmb) reveals sequence similarity with the serratia marcescens hemolysin genes (shla and shlb).we cloned a 13.5-kilobase ecori fragment containing the calcium-independent hemolysin determinant (pwpm110) from a clinical isolate of proteus mirabilis (477-12). the dna sequence of a 7,191-base-pair region of pwpm110 was determined. two polypeptides are encoded in this region, hpmb and hpma (in that transcriptional order), with predicted molecular masses of 63,204 and 165,868 daltons, respectively. a putative fur-binding site was identified upstream of hpmb overlapping the -35 region of the pr ...19902407716
the imaging of body asymmetry and hemihypertrophy.localized soft tissue and bone overgrowth may be the result of many different causes and will vary in severity, from involvement of a single digit to one half of the body. there are a variety of causes, including chronic lymphedema; lymphangiomatous malformations; neurofibromatosis; vascular malformations including the klippel-trenaunay-weber syndrome; macrodystrophia lipomatosa; multiple enchondromatosis and maffucci's syndrome. more recently, the bannayan syndrome and the proteus syndrome have ...19901963062
[an intravesical foreign body--the cause of chronic pyelonephritis in a child].the paper reports on a case of chronic pyelonephritis with bacillus proteus, generated by an intravesical foreign body (sewing needle). the needle was extracted with surgical cystoscope. after removing the foreign body, nalidixic acid was administered. the immediate evolution of the case was good.19901966514
tissue-binding affinity of proteus mirabilis fimbriae in the human urinary tract.binding characteristics of the two major fimbrial hemagglutinin types of uropathogenic proteus mirabilis were determined in frozen sections of human kidney and in exfoliated uroepithelial cells. p. mirabilis 3087, which expresses the mr/p fimbriae, adhered avidly to the tubular epithelial cells of the kidney and also to the epithelial cells of urinary sediment. no adhesion to glomerular or peritubular elements of the kidney was detected. indirect immunogold silver staining also showed that the p ...19901976113
female urinary tract infection in primary health care: bacteriological and clinical characteristics.female patients with symptoms of urinary tract infection (n = 1136) were studied in primary health care with respect to (a) clinical symptoms as predictors of bacteriuria; (b) relation between aetiological agent and clinical picture, especially for p-fimbriated escherichia coli; and (c) clinical findings in cases with 10(2)- less than 10(5) cfu/ml of e. coli. prevalence of bacteriuria (greater than or equal to 10(5) cfu/ml) was 61%. concurrence of urgency/frequency and dysuria, short duration of ...19901977196
lactate dehydrogenase isoenzyme electrophoresis as a typing method for enterobacter cloacae, klebsiella oxytoca and proteus mirabilis. 19901979580
[the effect of catecholaminergic agents on the efficacy of immunostimulants].the influence of combinations of immunostimulants (prodigiosin, methyluracil, levamisole) and catecholaminergic agents (dopamine, haloperidol, noradrenaline, phentolamine, isoproterenol, propranolol) on the results of antibiotic therapy of acute generalized proteus infection and primary immune response to ram erythrocytes was studied on outbred albino mice. it was established that the agents influencing dopaminergic, alpha- and beta-adrenergic receptors can in some cases change the efficiency of ...19901981988
kinetics of haemolytic activity in proteus strains.proteus species produces toxins and constitutes a causative agent of some chronic and recurrent infections. for the study of haemolytic activity and the production and inhibition kinetics, a total of 140 local isolates were diagnosed and examined by the general biochemical methods, and their ability of haemolysis were tested by both direct and indirect methods utilizing the enrichment procedure for all strains. two antibiotics, erythromycin and keflex (cephalexin), were tested for the study of h ...19901715649
coenzymatic properties of low molecular-weight and macromolecular n6-derivatives of nad+ and nadp+ with dehydrogenases of interest for organic synthesis.the catalytic activity, expressed as km and vmax values, of 16 enzymes of practical interest with the macromolecular coenzymes poly(ethylene glycol)-n6-(2-aminoethyl)-nad+ and poly(ethylene glycol)-n6-(2-aminoethyl)-nadp+ and their low molecular weight precursors n6-(2-aminoethyl)-nad+ and n6-(2-aminoethyl)-nadp+, was investigated. the enzymes examined are of direct interest for organic synthesis (i.e. alcohol dehydrogenase from yeast, horse liver, or thermoanaerobium brockii, lactic dehydrogena ...19901366782
[urinary infection in patients with obstructive uropathies].obstructive uropathies are among the most frequent predisposing factors in urinary infection. we have reviewed the frequency of pathogenes causin urinary infection in obstructive uropathies. we have exmined 259 patients with prostatic adenoma and urinary calculosis prior and after the operation during the years 1986 and 1987. the most frequent are bacteria: proteus, pseudomonas, klebsiella and e. colli. proteus was the most frequent (35.2 p.c.), and then pseudomonas (24.5 p.c.) and e. coli (10 p ...199017977397
postpartum uterine flora following normal and abnormal puerperium in cows.uterine swabs were taken from the uteri of three groups of cows (n = 5 each) on days 1 and 2 and then at 3-d intervals up to 32 d post partum. group i cows had a normal delivery, group ii cows had dexamethasone-induced parturition, and group iii cows were subjected to a caesarean section. all cows in group iii and two cows in group ii retained their fetal membranes. a total of 230 bacterial isolates were made, and the most frequently isolated bacteria were escherichia coli, streptococci, proteus ...199016726838
ytr-830h: its origin, the present and the future. 198916312317
comparative in vitro antibacterial activities of cefetamet (ro 15-8074/005), a new oral cephalosporin, and eight other oral antimicrobials. 198916312332
antimicrobial activity of a new difluorinated quinolone: lomefloxacin (sc-47111). 198916312351
sensitivity to beta-lactams and aminoglycosides of clinically important pathogens isolated from children in poland. 198916312442
interaction between antibiotics and non-specific humoral immunity. 198916312465
bacteriological study of extracted iucds.previous studies have shown the users of intrauterine devices have a 4.4 times greater risk of pelvic inflammatory disease. this present study intends to categorize the distribution of microbes in cultures from different types of extracted copper intrauterine devices (iucds). bacteriological studies of aerobic and anaerobic organisms were performed on the extracted iucds of 522 iucd users; 480 wearing a multiload cu 250, 15 wearing a copper t, 22 lippes loop and 5 a copper 7. cultures were ne ...198912283073
phylogenetic stains: ribosomal rna-based probes for the identification of single cells.rapid phylogenetic identification of single microbial cells was achieved with a new staining method. formaldehyde-fixed, intact cells were hybridized with fluorescently labeled oligodeoxynucleotides complementary to 16s ribosomal rna (rrna) and viewed by fluorescence microscopy. because of the abundance of rrna in cells, the binding of the fluorescent probes to individual cells is readily visualized. phylogenetic identification is achieved by the use of oligonucleotides (length 17 to 34 nucleoti ...19892466341
occurrence and functional compatibility within enterobacteriaceae of a trna species which inserts selenocysteine into protein.the selc gene from e. coli codes for a trna species (trna(ucaser] which is aminoacylated with l-serine and which cotranslationally inserts selenocysteine into selenoproteins. by means of southern hybridization it was demonstrated that this gene occurs in all enterobacteria tested. to assess whether the unique primary and secondary structural features of the e. coli selc gene product are conserved in that of other organisms, the selc homologue from proteus vulgaris was cloned and sequenced. it wa ...19892470027
elevated serum prostate-specific antigen due to acute bacterial prostatitis.elevated serum prostate-specific antigen secondary to acute bacterial prostatitis occurred in 2 patients. this cause of increased psa has not been documented previously.19892471345
epitope specificities of murine monoclonal and rabbit polyclonal antibodies against enterobacterial lipopolysaccharides of the re chemotype.murine monoclonal and rabbit polyclonal antibodies raised against the lipopolysaccharides (lps) of re mutants of salmonella minnesota, proteus mirabilis, and escherichia coli were serologically characterized. using natural re lps and natural and synthetic partial structures thereof, representing the 3-deoxy-d-manno-2-octulosonic acid (kdo) or lipid a region or both, the epitope specificities of four monoclonal antibodies were defined. clones 20 (immunoglobulin m [igm]) and 25 (igg3) recognize a ...19892474499
[erythrocytic diagnostic agents for determining antibodies to proteus o antigens].highly sensitive and specific erythrocyte diagnostic agents (ed) for the determination of antibodies to proteus o-antigens have been obtained by the sensitization of formolated sheep red blood cells (spbc) with activated lipopolysaccharides (lps) without the use of mediators. the tannin treatment of formolated srbc and/or the increase of temperature from 45 degrees c to 100 degrees c in the process of the preparation of ed have been found to produce no increase in effectiveness. antibody ed perm ...19892476896
staining bacterial flagella easily.a wet-mount technique for staining bacterial flagella is highly successful when a stable stain and regular slides and cover slips are used. although not producing a permanent mount, the technique is simple for routine use when the number and arrangement of flagella are critical in identifying species of motile bacteria.19892478573
patterns of mutations and selection in antibodies to the phosphocholine-specific determinant in proteus morganii.the contribution of somatic mutation to the generation of an antibody response was investigated by using the phosphocholine (pc) determinant in the bacterium proteus morganii as the model ag. the response to this determinant is restricted to a single vh/vl pair and apparently is derived from only one or two precursors per mouse. in this study we examined hybridoma antibodies from nine individual mice which produced representatives of 12 different clones. we found that all antibodies reactive wit ...19892478633
immunoelectron microscopy of proteus vulgaris by the plasma polymerization metal-extraction replica method: differential staining of flagellar (h) and somatic (o) antigens by colloidal golds.flagellar (h) and somatic (o) antigens of proteus vulgaris were differentially stained with antibodies coupled to different sizes of colloidal gold particles, and the distribution of these antigens was visualized by the plasma polymerization metal-extraction replica (pmr) method. the h antigen, labeled with 5 nm colloidal gold, was almost exclusively located on the flagella, whereas the o antigen, labeled with 10 nm colloidal gold, was almost exclusively located on the bacterial body. the marker ...19892479701
[structure of an o-specific polysaccharide of proteus mirabilis 03, containing n-(2-hydroxyethyl)-d-alanine].o-specific polysaccharide, obtained by mild acid degradation of the proteus mirabilis 03 lipopolysaccharide, was dephosphorylated with 48% hf to give a linear polysaccharide and an amino acid, n-(2-hydroxyethyl)-d-alanine. the structure of the polysaccharide was determined by methylation, the smith degradation and computer-assisted analysis of the 13c nmr spectra of original and dephosphorylated polymers and oligomers. the structure of the amino acid was elucidated by using 1h and 13c nmr spectr ...19892483615
[sensitivity to antibiotics of bacterial strains isolated from clinical specimens during the years 1985-1986].sensitivity of 312 strains of staphylococci, 386 strains of streptococci and 1193 strains of aerobic gram-negative bacilli to the selected antibiotics was tested. these strains were isolated from the clinical material at the clinical hospital no. 1 in warsaw within 1985-1986. staphylococci were sensitive to pristinamycin, cefazolin, fusidic acid, oxacillin, and clindamycin. in 1986, a decrease in the number of strains sensitive to these antibiotics, except cefazolin, was seen. in case of strepto ...19892485892
resistance patterns of bacterial isolates from wound infections in a university teaching hospital.a study of three hundred and sixty-five wound infections was carried out by aerobic and anaerobic methods. sensitivity testing of the isolates was done by disc diffusion method. it was found that gram negative bacteria predominated in the aetiology of the would infections. in the post-operative would sample, 55.73% grew single bacteria spp., while 44.27% grew more than one spp. most of the coliforms isolated showed marked resistance to some commonly used and relatively safe antibacterial agents. ...19892486768
[xanthogranulomatous pyelonephritis in an adolescent].one case of xanthogranulomatous pyelonephritis diagnosed following nephrectomy is described, considering its outstanding characteristics such as male adolescent, right kidney, involvement with normal function and without pathological history for which there is no explanation to date. this disease usually occurs in women 60 years or older, suggesting a renal tumor. in 50% of the cases, urine cultures are positive for escherichia coli or proteus mirabilis; in accordance with different reports rena ...19892487745
treatment of acute urinary tract infection in children with pipemidic acid.urinary tract infection in children is still an important problem in uronephrology. the disease tends to develop recurrently and results in chronic progressive renal disease in the future. pipemidic acid is a bactericidal quinolone derivate, with a wide spectrum against gram positive and negative bacteria. compared with nalidixic acid, pipemidic acid proves to be more effective against pseudomonas, e. coli, alkaligenes and salmonella. thirty one cases with acute urinary tract infection had been ...19892488238
[susceptibility of clinically-isolated problem pathogenic bacteria to broad spectrum antibiotics].the action of broad-spectrum antibiotics investigated by disc diffusion method on 636 problem bacteria such as s. aureus, enterobacter, klebsiella, pseudomonas, e. coli, proteus that isolated from various clinical specimens. the highest and lowest ratio of susceptibility were as follows: s. aureus were found sensitive 90% to netilmicin, 27% to piperacillin, enterobacter species 78% to netilmicin, 25% to piperacillin, klebsiella 69% to amikacin, 20% to piperacillin, pseudomonas 66% to amikacin, 5 ...19892488944
[urinary infections: observations on the frequency of microbial isolation at the hospital environment and its surroundings].numerous studies have been carried out to specify bacterial frequencies in the urinary tract infections. in such researches the prevalence of gram negative bacterial species has generally emerged, the most part of these ones would be enterobatteriaceae (and among them e. coli has a special prominent position); on the other hand several authors have often found, among other bacterial groups (gram positive bacteria, pseudomonas etc.) involved in such pathologies, some differences (also remarkable) ...19892490398
[nosocomial outbreak of proteus penneri infections at a general hospital]. 19892490457
[epidemiologic and therapeutic aspects of bacteremic infections of the urinary tract].a survey was carried out of all urinary tract infections which developed bacteremia during an 18 month period. the number of episodes of bacteremia of urinary origin (buo) detected in our institution during that period was 108. the urinary tract was the most common origin of the cases of community acquired (ca) bacteremia and the third in the hospital acquired (ha) cases. in both types, the most frequent organism was e. coli (69.6% and 40%, respectively). the most common underlying diseases were ...19892490670
[evaluation of sen-sir, a new microsystem for the identification and determination of bacterial sensitivity].the use of semiautomatic methods for identification and study of bacterial sensitivity to antimicrobials is increasingly widespread in clinical microbiology. in the present study, the evaluation of a new micromethod (sen-sir, bbl) for the identification and determination of bacterial sensitivity is reported. 115 clinical isolates of enterobacteria, 10 strains of acinetobacter calcoaceticus and 57 gram positive cocci, previously identified as belonging to the geni staphylococcus and streptococcus ...19892490681
[evaluation of the treatment with oral ciprofloxacin: experience with 24 patients].we have evaluated the clinical effectiveness and possible side effects of oral ciprofloxacin therapy in 24 patients (11 females and 13 males). two episodes from overall 25 infections were excluded of clinical and microbiological evaluation but were assessed regarding possible side effects. in most patients, the clinical condition was stable at the beginning of therapy. only 3 patients had nonfatal diseases. seventeen episodes were urinary tract infections (four of them with concomitant bacteremi ...19892490722
[nosocomially acquired infection of the urinary tract].we have evaluated 283 consecutive hospital acquired urinary tract infections (hauti) in a university hospital (incidence 5.6% of admissions). in females, spontaneous, symptomatic and younger patient infections predominated, while in males hauti were mostly asymptomatic, after catheterization and in elderly patients. chronic nonfatal diseases--particularly neurologic disease and diabetes--, old age, previous antibiotic use, the postoperative period, and cancer were the major general predisposing ...19892490855
[urinary tract infection at the emergency service of a third level hospital].104 patients with (ui) non-complicated urinary infection, diagnosed at the emergency department of a third level hospital, are presented. the median age (x +/- de) was of 58.12 +/- 20, 48 years; 78 were female (73%) and 22 male. 81 had fever and/or micturitional syndrome, the rest of the patients were without symptoms. the most frequent germ was escherichia coli, which was isolated in 80 patients (84.6%) followed by proteus mirabilis in 9 patients (8.6%) and enterococo in 2 patients (1.9%), othe ...19892491027
[significant bacteriological findings in hiv-positive patients].twenty-four episodes of bacterial infections were identified over a 18 month period in 11 patients (8 with acquired immunodeficiency syndrome and 3 with aids related complex). eight of the 11 infected patients were drug abusers and 3 homosexual people. nosocomial bacterial infections were common in patients with aids and had high fatality rates. gram-negative bacteria resulted the most common micro-organisms (e.coli, proteus, enterobacter, serratia, klebsiella). the aztreonam treatment was very ...19892491436
[sepsis: clinical course study of 126 patients in an internal medicine department].126 cases of sepsis were retrospectively studied in an internal medicine department, giving special attention to the clinical evolution. 67 males and 59 females with a median age of 65 years old were discovered. 92% had one or more diseases, mainly cold (30%) and diabetes mellitus (28%). the septic sources were urinary (37%) and respiratory (31%). 84% of the germs were gram (-), mainly e. coli and proteus sp. a mortality rate of 36% was found, the primary rates being: eighth decade (52%), patien ...19892491519
[abscess of the psoas muscle and the thigh: report of a case]. 19892491564
the upper urinary tract. 19892492289
characterization of the structural genes for the dna-binding protein h-ns in enterobacteriaceae.the promoter region of escherichia coli hns, the structural gene for the dna-binding protein h-ns, has been identified by use of a promoter search vector and the in vivo transcriptional start point by primer extension analysis. the homologous hns genes of two other enterobacteriaceae, proteus vulgaris and serratia marcescens, were identified by heterologous hybridization with a dna probe derived from e. coli hns, cloned and sequenced. taking into account only the invariant nucleotides and amino ...19892494066
prostaglandins may play a signal-coupling role during phagocytosis in amoeba proteus.phagocytosis in amoeba proteus can be induced with prostaglandins (pg). in addition, arachidonic acid (the fatty acid precursor to the pg-2 series) also induces phagocytosis. the induction of phagocytosis with arachidonic acid can be partially inhibited by the cyclooxygenase inhibitor indomethacin. phagocytosis in the amoeba can also be induced with the chemotactic peptide n-formylmethionyl-leucylphenylalanine (nfmlp). the peptide presumably induces phagocytosis by interacting with receptors on ...19892495854
production of antibody to and cellular localization of erythrocyte-sensitizing substance from rickettsia rickettsii.antibodies to rickettsia rickettsii erythrocyte-sensitizing substance (ess) were raised in rabbits by using a derivatized ess. the resulting antibodies reacted with r. rickettsii and cross-reacted with rickettsia conorii, a member of the spotted fever group rickettsiae, but did not react with rickettsia typhi, a member of the typhus group rickettsiae, legionella bozemanii, or proteus vulgaris ox19 or ox2. immunoblot analysis indicated that ess was present in more than one fraction and that the m ...19892497137
antimicrobial effectiveness of six paradols. 1: a structure-activity relationship study.the pattern of antimicrobial effectiveness of (0)-, (2)-, (3)-, (4)-, (8)- and (9)-paradols was studied. (3)- paradol was more active than the other homologues with a minimum inhibitory concentration (mic) of 1 mg/ml when tested against proteus vulgaris, pseudomonas aeruginosa, staphylococcus aureus and botryodiplodia theobromae. at 0.2 mg/ml, (3)-paradol completely inhibited the spore germination of trichophyton mentagrophytes and after 3 h of exposure at 0.5 mg/ml, it inhibited the growth of a ...19892497275
[the rapid identification of e. coli by the detection of 3 enzymes].232 isolates of gram-negative, oxidase-negative bacteria, isolated from various samples of different animal species, were tested with help of rapidec coli for the production of beta-glucuronidase, beta-galactosidase and indole. the test correctly identified 164 of 175 e. coli strains (sensitivity 93.7%) and correctly indicated that 57 of 57 isolates of the family enterobacteriaceae (7 citrobacter sp., 18 enterobacter sp., 16 klebsiella sp., 10 proteus sp., 2 providencia sp. and 4 salmonella sp.) ...19892497726
evaluation of a disk approximation test of inducible beta-lactamases in enterobacteriacae and pseudomonas aeruginosa.a modified disk approximation test of inducible beta-lactamases in enterobacteriacae and pseudomonas aeruginosa was evaluated. the amount of inducer was adapted to produce the smallest possible zone of inhibition and the distance between the centre of the disks was standardized. among several beta-lactam antibiotic disks tested, imipenem (0.06 microgram per disk) or cefoxitin (10 micrograms per disk) placed at a distance of 14-16 mm from a cefotaxime disk (30 micrograms) most efficiently reveale ...19892497754
complete secretion of activable bovine prochymosin by genetically engineered l forms of proteus mirabilis.to circumvent problems encountered in the synthesis of active chymosin in a number of bacteria and fungi, a recombinant dna l-form expression system that directed the complete secretion of fully activable prochymosin into the extracellular culture medium was developed. the expression plasmid constructions involved the in-frame fusion of prochymosin cdna minus codons 1 to 4 to streptococcal pyrogenic exotoxin type a gene (spea') sequences, including the spea promoter, ribosomal binding site, and ...19892499253
[antagonistic action of pseudomonas aeruginosa against staphylococci, coliform bacilli and various types of proteus].antagonistic activity of 2 fresh isolates and 3 collection strains of pseudomonas aeruginosa against 177 microbial strains was determined with the method of late antagonism. among the microbial strains there were 56 staphylococcal strains isolated from patents and carriers. 38 nontypable colon bacilli isolated from healthy persons, 59 enteropathogenic colon bacilli of various serogroups, 12 strains of proteus and 12 colon bacilli, carriers of multiple drug resistance factors (r factors). all the ...19892499284
[urease activity of bacteria in urine].urea splitting bacteria are related to the formation of struvite or apatite. we investigated the urease activity of bacteria by two methods; the direct measurement of urease activity of viable bacteria and sonicated bacteria from amounts of ammonia by the indophenol method, and the measurement of urease activity by alkalization of infected urine. proteus mirabilis and pseudomonas aeruginosa had moderate activity of urease, and morganella morganii and staphylococcus epidermidis had the most power ...19892500012
monoadduct forming photochemical reagents for labeling nucleic acids for hybridization.we describe the synthesis of three angelicin derivatives which can be used for labeling nucleic acids with biotin. these compounds were used to label nucleic acids in the presence of lysed cell constituents. the resulting labelled nucleic acids show hybridization to a genus specific probe for e. coli. the relative comparison of sensitivity indicates that a polyamine linker is better than a polyethylene oxide linker between the biotin and angelicin moieties.19892500642
effects of selected carbohydrates and the contribution of the prophenoloxidase cascade system to the adhesion of strains of pseudomonas aeruginosa and proteus mirabilis to hemocytes of nonimmune larval galleria mellonella.the adhesion of strains of pseudomonas aeruginosa and proteus mirabilis to the plasmatocytes and granular cells of nonimmune larval galleria mellonella was influenced by and varied with the type of carbohydrate. laminarin enhanced prophenoloxidase activation and bacterial adhesion to the hemocytes whereas sucrose suppressed both activities. for all other sugars there was no correlation between bacterial adhesion to the hemocytes and phenoloxidase activity. it is proposed that bacterial adhesion ...19892501016
microbiological quality and public health significance of ice-cream.fifty samples of ice-cream were subjected to microbiological examination. of these 72% had total viable count over 10(6)/g while 66% had coliform count between 10(2)-10(3)/g. the micro-organisms isolated were escherichia coli (46%), enterobacter aerogenes (34%), staphylococcus aureus (26%), proteus species (16%), streptococcus faecalis (12%), citrobacter species (10%) and bacillus cereus (4%). the results showed that commercially prepared ice-cream sold in the market was not satisfactory for hum ...19892501524
[bactericidal spectrum of pyocins on strains collected at region ix and x hospitals in chile].the inhibitory effect of 5 pyocins produced by wildstrains of ps aeruginosa was tested by qualitative and quantitative techniques. 313 strains of 4 species: e coli, p vulgaris, ps aeruginosa and st aureus were assayed. it was shown that the most active pyocin was p37, which inhibited 276 out of 313 strains tested. about 80% of e coli and 90% of st aureus strains were inhibited by the pyocins under being tested. it was established that sensitivity to pyocin was not restricted to close-related spe ...19892501848
synthesis and biological activity of 7 alpha-hydroxyethyl-1-oxacephem derivatives.a series of 7 alpha-hydroxyethyl-1-oxacephems (1) was synthesized. the main focus of this study was to investigate biological activity relationships between 1-oxacephems (1) and the corresponding cephems (2). replacement of the sulfur atom of 2 by the oxygen atom caused an enhancement of antibacterial activity, although the antibacterial activity of 1 was not high enough. additionally 1 showed beta-lactamase inhibitory activity, especially against cephalosporinase. however, the potency was lower ...19892502525
rapid flow cytometric bacterial detection and determination of susceptibility to amikacin in body fluids and exudates.a flow cytometry-based method for rapid and quantitative detection of bacteria in various clinical specimens and for rapid determination of antibiotic effect is described. achieving such a measurement with high sensitivity required discrimination between bacteria and other particles which were often present in clinical samples in high concentrations. this discrimination was facilitated by detecting the bacterial characteristic light scatter and fluorescence signals following staining, e.g., with ...19892502559
[the sequence of specific immune reactions at the biliary level after oral vaccination].the appearance and the progression of the specific immunoreactions at biliary levels (agglutinant titre, immunoenzimatic titration of igagm, iga, igg) in rabbits treated per os from 1 to 10 times, daily or bi-weekly, with 20 and 40 milliard of inactivated bacterial antigens (e. coli, proteus vulgaris, staph. aureus, str. faecalis) have been studied. the appearance of the specific biliary antibodies has been very precocious (only one day after the first administration) and the values increased pr ...19892503788
providencia alcalifaciens and travellers' diarrhoea. 19892504344
in vitro antibacterial properties of cefetamet and in vivo activity of its orally absorbable ester derivative, cefetamet pivoxil.the in vitro activity of cefetamet, the microbiologically active metabolite of the orally administered prodrug cefetamet pivoxil, was compared with that of cephalexin, cefaclor, cefuroxime and amoxicillin. cefetamet was highly active against enterobacteriaceae, neisseria spp., vibrio spp., haemophilus influenzae and streptococci other than enterococci. cefetamet inhibited cefaclor-resistant species such as proteus vulgaris, providencia stuartii, providencia rettgeri and serratia marcescens. stap ...19892504597
comparative activity of cefixime and cefaclor in an in vitro model simulating human pharmacokinetics.the dependence of the antibacterial activity of the two oral cephalosporins cefixime and cefaclor on pharmacokinetic properties was investigated in an in vitro model using strains of enterobacteria and a streptococcal strain. in the cultures the course of serum concentrations of the respective antibiotic was simulated. the more rapidly attained (1 h) high peak levels (17.5 micrograms/ml) of cefaclor (500 mg dose) in no case showed an advantage over the more slowly reached (3 h) low peak levels ( ...19892504600
surveillance of antibiotic resistance in south east asia.antibiotic resistance in gram-negative bacteria, particularly salmonella and shigella, requires surveillance worldwide. this study describes results of surveys in hong kong, bangkok and kuala lumpur. all strains were isolated in hospitals which have large community catchment areas in addition to specialised hospital units. the prevalence of resistant strains was high in all areas. gram-negative bacteria such as enterobacter associated with hospital infections were resistant to penicillins and ce ...19892504618
[the first case of tsutsugamushi disease in ehime prefecture].the first case of tsutsugamushi disease in ehime prefecture was experienced in december 1987 with successful isolation of the causative agent. the patient was taken ill twelve days after infection. immunofluorescent antibody tests using the isolate, yamazaki strain, and gilliam, karp, kato, irie and shimokoshi strains as antigens revealed that the specific antibodies against these antigens appeared and increased in the blood of the patient during the course of the disease. and the antibody titer ...19892504840
urinary tract infection.over two years, 9892 mid-stream urine samples from patients attending the aga khan university hospital, karachi were cultured. significant bacterial growth was seen in 23.5% samples. further identification of these organisms revealed 40% of e. coli, 16% pseudomonas aeruginosa, 11% klebsiella aerogenes, 5.0% enterobacter sp., 13% proteus sp., 4.0% serratia liquifaciens, 1.0% acinetobacter sp., 3.0% citrobacter sp., 4.0% enterococci, 0.5% staphylococcus aureus. results of sensitivity tests perform ...19892504948
lytic effect of pseudomonas aeruginosa elastase on gram-positive and -negative bacteria.elastase of pseudomonas aeruginosa p3 caused lysis of freshly grown cells of gram-positive (staphylococcus aureus and s. epidermidis) and gram-negative (proteus vulgaris and salmonella typhi) bacteria. heat killed and lyophilized gram-negative bacteria showed higher sensitivity to elastase. both gram-negative and gram-positive bacteria were lysed maximally by elastase at ph 8.0. at this ph, the activity of elastase was maximal in tris-hcl and glycine-naoh buffers followed by tris-maleate and cac ...19892505788
biocidal activity of ozone versus formaldehyde against poultry pathogens inoculated in a prototype setter.ozone was evaluated as an alternative hatchery disinfectant to replace formaldehyde in the event that the environmental protection agency regulates the use of formaldehyde under the toxic substances control act. cultures of staphylococcus, streptococcus, and bacillus species previously isolated from poultry hatcheries and selected culture collections of escherichia coli, pseudomonas fluorescences, salmonella typhimurium, proteus species, and aspergillus fumigatus were spread-plated on open petri ...19892506541
[susceptibility of clinical isolates to aztreonam].in vitro antibacterial activities of 9 antibiotics including aztreonam (azt) against clinically isolated gram-negative bacteria were determined using mic-2000 plus system. bacteria were isolated from clinical materials in saga medical school during a period from may 1987 to march 1988. summarized results were as follows: 1. azt showed excellent antibacterial activities against escherichia coli, klebsiella pneumoniae, proteus sp. and haemophilus influenzae, and mic80 values of azt against these o ...19892507799
[in vitro antibacterial effect of a new oral cephalosporin, cefixime. results of a multicenter study].minimal inhibitory concentrations (mic) of cefixime (cxm) were evaluated by agar dilution against 2,469 bacterial strains isolated in 10 hospitals. for enterobacteriaceae, mic 50 and 90% micrograms/ml were respectively: (i) naturally non beta lactamase producing species: e. coli and shigella 0.25-0.5; salmonella 0.06-0.25; p. mirabilis 0.008-0.032. (ii) chromosomal penicillinase producing species: klebsiella 0.06-2. (iii) chromosomal cephalosporinase producing species: e. cloacae and c. freundii ...19892508039
[determination of bactericidal minimum concentrations of 3 antiseptics and 1 disinfectant on 580 hospital gram-negative bacilli].minimal bactericidal activities (mbcs) of three antiseptics (povidone iodine, chlorhexidine digluconate, benzalkonium chloride) and one disinfectant (sodium hypochloride) where determined, on 580 hospital gram negative bacilli. previously the afnor t 72-150 standard for antiseptic and disinfectant was established for two reference strains e. coli cip 54 127 and p. aeruginosa cip a 22. no difference was found between the mbc obtained with these strains in afnor standard and in microdilution metho ...19892508042
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