Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| complete reconstitution of conjugation and subsequent degradation of the tumor suppressor protein p53 by purified components of the ubiquitin proteolytic system. | the wild-type tumor suppressor protein p53 is a short-lived protein that plays important roles in regulation of cell cycle, differentiation, and survival. mutations that inactivate or alter the tumor suppressor activity of the protein seem to be the most common genetic change in human cancer and are frequently associated with changes in its stability. the ubiquitin system has been implicated in the degradation of p53 both in vivo and in vitro. a mutant cell line that harbors a thermolabile ubiqu ... | 1994 | 8034027 |
| the inter-relationship of herpes virus, papilloma 16/18 virus infection and pap smear pathology in israeli women. | a previous survey of the seroprevalence of the herpes virus type 2 (hsv-2) infection in israel provided us with an opportunity to study a) the prevalence of human papillomavirus (hpv) 16/18 in selected groups of women; b) the correlation between the infection with hsv-2, hpv-16/18 and papanicolaou (pap) pathology; and c) to identify groups of women who might benefit from routine pap screening. four different population groups of women aged 17-60 years were studied: groups 1 and 2 comprised healt ... | 1994 | 8034488 |
| persistence of type-specific human papillomavirus infection among cytologically normal women. | 1994 | 8035046 | |
| repeatability of interview-derived information on sexual history: a study in women. | we assessed the repeatability of interview-derived information on age at first sexual intercourse and number of sexual partners in women participating in an ongoing prospective study of genital human papillomavirus infection in toronto, canada. of the 100 study participants invited to attend for re-interview twice during their first year in the study, 74 attended for the first follow-up interview about 5 months after recruitment, and 28 of these 74 subjects attended for a second interview about ... | 1994 | 8038255 |
| the value of primary colposcopy in genitourinary medicine--a six year review. | to evaluate the effectiveness of primary colposcopy in the genitourinary medicine setting by comparing the cervical cytology and punch biopsy results for women identified as having an abnormal cervical transformation zone. | 1994 | 8039784 |
| development of human papillomavirus-associated buschke-löwenstein penile carcinoma during cyclosporine therapy for generalized pustular psoriasis. | 1994 | 8040428 | |
| toward objective quality assurance in cervical cytopathology. correlation of cytopathologic diagnoses with detection of high-risk human papillomavirus types. | using the bethesda system, five pathologists independently diagnosed 200 smears that originally had been classified as "atypical," and the results were correlated with concurrent detection of human papillomavirus (hpv) dna by southern analysis and by polymerase chain reaction amplification. the smears were reclassified as benign reactive changes (negative), atypical squamous cells of undetermined significance, or squamous intraepithelial lesion (sil). exact five-way cytologic agreement was achie ... | 1994 | 8042586 |
| allelotype analysis of cervical carcinoma. | to identify the genetic events which may play a role in the development of cervical carcinoma, we performed a detailed allelotype analysis utilizing dna from 53 primary tumors and corresponding normal cells and 57 polymorphic probes mapped to each of the chromosomal arms, excluding the short arms of the acrocentric chromosomes. loss of heterozygosity (loh) of > 25% was observed at sites on 11 chromosomal arms, which included 1q (26%), 3p (35%), 3q (31%), 4q (46%), 5p (53%), 5q (38%), 6p (28%), 1 ... | 1994 | 8044799 |
| hpv-16 e6 and e7 genes, like sv40 early region genes, are insufficient for immortalization of human mesothelial and bronchial epithelial cells. | simian virus 40 (sv40)-transformed human cells usually have a period of increased proliferative potential that terminates in culture crisis, after which immortalization may or may not occur. this study addresses the question of whether human papillomavirus (hpv)-transformed cells may also undergo crisis. two types of human epithelial cells were transfected with either hpv-16 e6 and e7 genes or sv40 early region genes, and all transfectants underwent at least 20 more population doublings than the ... | 1994 | 8050498 |
| lack of synergistic association between human papillomavirus and ras gene point mutation in laryngeal carcinomas. | the spectrum of human papillomavirus (hpv) subtypes in laryngeal carcinomas was investigated by combined polymerase chain reaction (pcr) and restriction fragment length polymorphism analysis. hpv dna was detected in 11 (24%) of 45 cases, including hpv 16 in 9 cases and hpv 18 in 2 cases. other hpv subtypes commonly found in the female genital organs were not detected. in addition, the point mutation of codons 12 and 13 in c-ki-ras-2, c-ha-ras-1, and n-ras genes was studied by the pcr-single-stra ... | 1994 | 8052064 |
| functions of human papillomavirus e6 and e7 oncoproteins. | the identification of certain human papillomaviruses as human tumour viruses has been paralleled by our understanding of how the viral oncoproteins function. the virally encoded e6 and e7 proteins act, in part, to abrogate the activities of the tumour suppressor proteins p53 and prb. the interaction between these viral proteins and regulators of cell growth offers targets for future therapeutic developments. | 1994 | 8055181 |
| [typification of a subgroup of cervical papilloma by in situ hybridization. preliminary study]. | the purpose of this study was to analyze 19 patients infected with human papilloma virus (hpv), in order to determine the most frequent subtype. subjects were patients of the national institute of perinatology (inper), between 1991-1992. all the patients had positive cytology, cervical biopsy was done through colposcopy control. sample characteristics were: mean age 29 years, married; most of them multiparous (9/19) and 7 were monogamous. six out of 12 cytologies were positive for hpv, 12 out of ... | 1994 | 8056363 |
| the ability of human papillomavirus e6 proteins to target p53 for degradation in vivo correlates with their ability to abrogate actinomycin d-induced growth arrest. | functional p53 protein is associated with the ability of cells to arrest in g1 after dna damage. the e6 protein of cancer-associated human papillomavirus type 16 (hpv-16) binds to p53 and targets its degradation through the ubiquitin pathway. to determine whether the ability of e6 to interact with p53 leads to a disruption of cell cycle control, mutated e6 proteins were tested for p53 binding and p53 degradation targeting in vitro, the ability to reduce intracellular p53 levels in vivo, and the ... | 1994 | 8057451 |
| functional studies of e7 proteins from different hpv types. | we have performed comparative studies on the e7 proteins from malignant and non-malignant human papillomavirus types hpv 1, 6, 11, 16, 18, 33). gst/e7 fusion proteins from all these hpv types associate with rb1, p107 and the cyclin a/cdk2 complex. as has been shown for rb1, the association with p107 and cyclin a was weaker for the 'low risk' hpv6 and 11 e7 proteins as compared to 'high risk' hpv16, 18 and 33 e7 proteins. in contrast the e7 protein of the benign type hpv1 bound rb1, p107 and cycl ... | 1994 | 8058327 |
| interaction of p53 with mdm2 is independent of e6 and does not mediate wild type transformation suppressor function. | using a new series of p53 mutants targeting the conserved regions we have analysed the relationship of various activities of the protein. mdm-2 and human papillomavirus (hpv) e6, two proteins which interact with and abrogate p53 function, were shown to bind independently. deletion of the conserved regions of the protein in which most of the naturally occurring mutations are found (boxes ii-v) abrogated transcriptional activity and the ability to interact with e6, supporting the importance of thi ... | 1994 | 8058335 |
| patients as subjects for research: ethical dilemmas for the primary care clinician-investigator. | past studies suggested an association between human papillomavirus (hpv) and cervical intraepithelial neoplasia (cin). in 1987, university of north carolina (unc) hospitals family practice center clinicians were approached for a control population to study this association. | 1994 | 8059623 |
| proliferative verrucous leukoplakia and verrucous hyperplasia. | proliferative verrucous leukoplakia and hyperplasia are irreversible clinicopathologic lesions of the oral cavity and upper respiratory tract with considerable potential for evolving into verrucous or other forms of squamous cell carcinoma. proliferative verrucous leukoplakia is a disease of the oral cavity in which verrucous hyperplasia is a part of its developmental spectrum. verrucous hyperplasia, at other head and neck mucosal sites, may be associated with papillomas or be a de novo lesion. ... | 1994 | 8060064 |
| human papillomavirus-related serological markers of invasive cervical carcinoma in brazil. | masked sera from 194 cases and 217 controls participating in a case-control study of cervical cancer in brazil were examined for antibodies to human papillomavirus (hpv) 16 e6 and e7 by radioimmunoprecipitation assay. radiolabeled full-length e6 and e7 proteins expressed by in vitro transcription and translation in rabbit reticulocyte lysate were used as antigens. the antibody prevalences in cases and controls were: 54.1% versus 6% for e6; 30.4% versus 4.6% for e7; 63.4% versus 10.1% for either ... | 1994 | 8061584 |
| presence of human papillomavirus dna in amniotic fluids of pregnant women with cervical lesions. | amniotic fluids from 37 pregnant women with cervical lesions indicative of human papillomavirus (hpv) infection, detected by histology and slot hybridization, were collected at different times during pregnancy and assayed for hpv using the polymerase chain reaction. hpv dna amplification was obtained in 24 of 37 samples using general primers. of the 24 positive cases, 13, 5, and 0 were of hpv types 16, 18, and 33, respectively, as determined by using specific anticontamination primers. a correla ... | 1994 | 8063239 |
| retinoic acid suppresses human papillomavirus type 16 (hpv16)-mediated transformation of human keratinocytes and inhibits the expression of the hpv16 oncogenes. | we have used a model system of normal hkc and hkc immortalized by transfection with hpv16 dna (hkc/hpv16) to investigate the effect of ra on the growth of hkc/hpv16 and the expression of the hpv16 oncogenes e6 and e7. these studies found that hkc/hpv16 are about 100-fold more sensitive than normal hkc to growth inhibition by ra in both clonal and mass culture growth assays. the precursor to ra, retinol, was also found to be a more potent inhibitor of growth of hkc/hpv16 than normal hkc while bet ... | 1994 | 8067286 |
| genital human papillomavirus infections in young women with vulvar and vestibular papillomatosis. | possible involvement of human papillomaviruses (hpv) in the development of vulvar and vestibular papillomatosis was investigated by using pcr to determine whether hpv dna was present in lesions. fourteen of 272 (5.1%) young women studied were found on gross and histological examination to have vulvar or vestibular papillomatosis. hpv dna sequences were detected in cervicovaginal lavage specimens of 2 of 14 (14.3%) papillomatosis patients and 1 of 17 (5.9%) matched individuals in the control grou ... | 1994 | 8070461 |
| diet and genital warts: a case-control study. | clinical observations support a substantial role for impaired immunity in the development of human papillomavirus (hpv) infections. intake of vitamins, especially vitamins a and c, and alcohol consumption have been reported to influence immune response. | 1994 | 8073343 |
| lethal asphyxiating juvenile laryngeal papillomatosis. a case report with human papillomavirus in situ hybridization analysis. | chronic, progressive, and recurrent laryngeal papillomatosis is an uncommon problem that primarily occurs in children and has been shown (in the pediatric age group) to be caused by human papillomavirus (hpv) types 6 and/or 11. juvenile laryngeal papillomatosis is hallmarked by recurrences, necessitating repeated surgical procedures to maintain airway patency, and is rarely encountered cause of sudden and unexpected death. this case describes a 22-month-old girl who had been treated for laryngea ... | 1994 | 8074107 |
| detection of high-risk human papillomavirus in the cervix and semen of sex partners. | to investigate the possibility of sexual transmission of human papillomavirus (hpv), 53 married couples were examined for the presence of hpv-16 and -18 dnas in the uterine cervix and semen using the polymerase chain reaction method. twenty-three of the 53 women and 12 of the 53 male partners were positive for hpv-16 dna. no hpv-18 dna was detected in samples from any of the partners. in 27 pairs, both partners were negative for hpv dna in cervix or semen; in the remaining 26 pairs, at least 1 o ... | 1994 | 8077728 |
| serological differentiation of human papillomavirus types 11, 16 and 18 using recombinant virus-like particles. | the l1 major capsid protein-coding sequences of human papillomavirus (hpv) types 11, 16 and 18 were expressed in the baculovirus system. virus-like particles (vlps) were purified from recombinant-infected spodoptera frugiperda sf9 cells and cell-free culture supernatants. rabbits immunized with purified vlps developed antibodies that reacted only with the specific vlp type used as the immunogen. in addition, rabbit antibodies raised against infectious hpv-11 virions only reacted with hpv-11 l1 v ... | 1994 | 8077946 |
| the cellular dna polymerase alpha-primase is required for papillomavirus dna replication and associates with the viral e1 helicase. | persistent infection by papillomaviruses involves the maintenance of viral dna as a nuclear plasmid, the replication of which requires host dna polymerases. the role of the cellular dna polymerase alpha-primase holoenzyme was probed by using soluble extracts from rodent cells that replicate bovine papilloma virus 1 and human papilloma virus 6b dna in the presence of the viral e1 helicase and the e2 transcription factor. monoclonal antibodies directed against the catalytic 180-kda subunit of poly ... | 1994 | 8078945 |
| bacterial vaginosis and cervical intraepithelial neoplasia. | in an attempt to investigate an association between the finding of clue cells in papanicolaou-stained (pap) smears and cervical intraepithelial neoplasia (cin), a total of 6150 smears from 1976 were re-investigated. | 1994 | 8079612 |
| penile intraepithelial neoplasia. specific clinical features correlate with histologic and virologic findings. | to evaluate the existence of the morphologic features specific for penile intraepithelial neoplasia (pin), 1000 male sexual partners of women with genital condyloma or intraepithelial neoplasia were studied. | 1994 | 8082079 |
| human papillomavirus in pterygium. | between august 1991 and 1992 consecutive pterygium cases and eight normal conjunctival tissues were examined for the presence of polyclonal human papilloma virus (hpv) antigen by the avidin-biotin-complex technique and 16 of the pterygium cases and 25 pc of the control conjunctivas revealed hpv positivity. in six of the hpv positive pterygium tissues, there were also an epithelial proliferation. in conclusion, hpv infection was not endemic, but sporadic in cukurova region, and it may be contribu ... | 1994 | 8082148 |
| subclinical human papillomavirus infection in a high-risk population. | human papillomavirus (hpv) infection, which has been linked to cervical dysplasia and carcinoma, causes one of the most frequently diagnosed sexually transmitted diseases (stds) in the united states. to determine the prevalence of subclinical hpv infection, to detect any association with dysplasia, and to investigate risk factors, the author studied 249 sexually active adolescent girls and young women in a high-risk population by questionnaire, pelvic examination, cytology, and dot blot nucleic ... | 1994 | 8083064 |
| prevalence of hpv dna in cervical specimens in women with renal transplants: a comparison with dialysis-dependent patients and patients with renal impairment. | the aim of this study was to assess the prevalence of human papillomavirus (hpv) dna in women who had received a renal transplant and to compare this with two control groups. women who had a functioning renal transplant for greater than 6 months (n = 69) were compared with women on maintenance dialysis (n = 89) and women with impaired renal function (creatinine 0.15-0.39 mmol/l) (n = 22). women were excluded if they had had a hysterectomy, were older than 65 years, or were not yet sexually activ ... | 1994 | 8084456 |
| [condyloma acuminatum in female urethra: a case report]. | a case of female intraurethral condyloma acuminata is present in this report. a 52-year-old woman visited our hospital with the chief complaint of miction pain and bloody secretions. thirty seven years previously, she had had a pudental tumor resected which was diagnosed as condyloma acuminata. physical examination revealed two child-finger-head sized, polypoid tumors which arose from about 5 mm inside the external urethral orifice. laboratory findings were negative for rapid plasma reagin card ... | 1994 | 8085526 |
| expression of transforming growth factor-beta 1 and -beta 2 in human papillomavirus (hpv)-associated lesions of the uterine cervix. | human papillomaviruses (hpv) are implicated in the multistep process of cervical carcinogenesis. transforming growth factor beta(tgf-beta) inhibits the proliferation of epithelial cells, and it has also been found to inhibit hpv gene expression in nontumorigenic epithelial cell lines. in the present study, we examined the expression of tgf-beta 1 and tgf-beta 2 protein immunohistochemically (ihc) in a series of 95 hpv-positive and hpv-negative lesions of the uterine cervix, with special emphasis ... | 1994 | 8088612 |
| human papillomavirus detection by hybrid capture and its possible clinical use. | to determine the sensitivity of the hybrid capture method for human papillomavirus (hpv) detection and potential clinical uses as a screening method for the identification of cervical intraepithelial neoplasia. | 1994 | 8089224 |
| [application of antisense oligonucleotides into treatment of human cervical carcinoma]. | antisense oligodeoxynucleotides (oligomers) have critical roles for drugs targeted specifically against oncogenes. we obtained evidence for the inhibitory effects of oligomers to the e6/e7 region of human papilloma virus (hpv) types 16 and 18 on cervical epithelial cells. these cells contained phk16 cells that was the human keratinocytes immortalized by hpv16 full genome transfection, and c4ii cells that was the cervical carcinoma cells harboring hpv 18 genome. the oligomers greatly reduced both ... | 1994 | 8089611 |
| prevalence of human papillomavirus dna in female cervical lesions from rio de janeiro, brazil. | a hundred-sixty paraffin-embedded specimens from female cervical lesions were examined for human papillomavirus (hpv) types 6, 11, 16 and 18 infections by non-isotopic in situ hybridization. the data were compared with histologic diagnosis. eighty-eight (55%) biopsies contained hpv dna sequences. in low grade cervical intraepithelial neoplasias (cin i), hpv infection was detected in 78.7% of the cases, the benign hpv 6 was the most prevalent type. hpv dna was detected in 58% of cin ii and cin ii ... | 1994 | 8524058 |
| a comparison of uterine cervical cytology and biopsy results: indications and outcomes for colposcopy. | there is some debate in the literature over the proper approach to the patient with a mildly abnormal cervical cytologic finding. one current approach for handling low-grade cytologic abnormalities is to perform colposcopy and biopsy if atypia, human papillomavirus (hpv) changes, or mild dysplasia is noted on cytologic examination. if a papanicolaou (pap) smear shows inflammation without atypia, the test is repeated after 3 months, and if inflammation does not clear, colposcopy is performed. thi ... | 1994 | 8289050 |
| human papillomavirus type 11 e2 proteins repress the homologous e6 promoter by interfering with the binding of host transcription factors to adjacent elements. | the e6 promoter of human papillomaviruses (hpvs) trophic for epithelia for the lower genital tract and the upper respiratory tract is regulated in vitro by homologous and heterologous papillomaviral e2 proteins that bind to a consensus responsive sequence (e2-rs) accn6ggt. when hpv type 11 (hpv-11) expression is examined in epithelial cell lines, the hpv-11 e2-c protein, which lacks the amino-terminal transactivating domain of the full-length e2 protein, invariably represses the homologous viral ... | 1994 | 8289341 |
| interaction of human papillomavirus (hpv) type 16 capsid proteins with hpv dna requires an intact l2 n-terminal sequence. | encapsidation of papillomavirus dna involves dna-protein and protein-protein interactions. we sought to define the role of each human papillomavirus (hpv) capsid protein in hpv dna encapsidation. hpv16 major (l1) and minor (l2) capsid proteins purified from recombinant vaccinia virus-infected cells were compared for their ability to bind nucleic acids. l2 protein, but not l1 protein, could bind hpv dna. to map the dna-binding region of l2, a series of truncated or point-mutated l2 protein open r ... | 1994 | 8289365 |
| human cancer cell lines express a negative transcriptional regulator of the interferon regulatory factor family of dna binding proteins. | members of the interferon regulatory factor (irf) family of dna binding transcription factors have roles in growth regulation, antiviral responses, and transcriptional induction of interferon (ifn)-activated early response genes. the irf family member isgf3 gamma is the dna binding component of ifn-stimulated gene factor 3 (isgf3), a multicomponent complex responsible for the stimulation of ifn-alpha-responsive genes. ifn-alpha-stimulated formation of isgf3 and subsequent gene expression can be ... | 1994 | 8289823 |
| dna damage induced p53 mediated transcription is inhibited by human papillomavirus type 18 e6. | cervical cancer is similar to other human cancers in that it develops through a multistep process. however, infection with oncogenic human papillomaviruses (hpvs) is believed to be essential for the initiation of this disease. although hpv may play a central role in the early stages of neoplasia, the accumulation of mutations in an assortment of genes precedes the development of malignant cervical carcinoma. the mechanisms by which abnormalities accumulate are various, but it is possible that vi ... | 1994 | 8290274 |
| pseudo oral hairy leukoplakia in a renal allograft recipient. | oral hairy leukoplakia (ohl) is a disorder of the tongue associated with epstein-barr virus (ebv). ohl is seen mainly in hiv infection but is also rarely seen in the course of iatrogenic immunosuppression, especially in kidney transplantation; ohl is even more rarely seen in immunocompetent hosts. lesions that clinically and histologically mimicked ohl but were not associated with ebv were recently characterized as pseudo hairy leukoplakia. we present such a case that occurred in a renal allogra ... | 1994 | 8294586 |
| the p53 tumor suppressor gene frequently is altered in gynecologic cancers. | mutation of the p53 tumor suppressor gene, often accompanied by overexpression of mutant p53 protein, is the most frequent molecular genetic event described thus far in human cancers. in adenocarcinomas of the ovary and endometrium, p53 overexpression is seen in approximately 10% to 15% of early and 40% to 50% of advanced cancers. similar to many other types of human cancers, ovarian and endometrial cancers that overexpress p53 protein contain mutations in conserved regions of the p53 gene. thes ... | 1994 | 8296829 |
| laryngeal verrucous carcinoma: a clinicopathologic study and detection of human papillomavirus using polymerase chain reaction. | laryngeal verrucous carcinoma (lvc) is a rare, well-differentiated variant of squamous carcinoma with a low malignant potential. human papillomavirus (hpv)-16 dna has been identified in a small number of lvc and an etiologic relationship has been suggested. a correlative clinical and molecular pathological study was performed in order to determine the prevalence and typing of hpv dna in lvc. possible associations between patient and tumor subsets, and the presence of hpv dna were also investigat ... | 1994 | 8302116 |
| p53 mutations in hpv-negative cervical carcinoma. | human papillomavirus (hpv) infection has been strongly linked to the development of cervical carcinoma. two viral oncoproteins, e6 and e7, produced by hpv, have been shown to immortalize primary human genital epithelial cells by interacting with the protein products of cellular tumor suppressor genes p53 and rb, respectively. e6 binds to the cellular p53 protein promoting p53 degradation and inactivity. this mechanism has been suggested to contribute to the oncogenesis of hpv-positive anogenital ... | 1994 | 8302581 |
| bowen's disease of the nail bed and periungual area. a clinicopathologic analysis of seven cases. | this article describes the clinical and histologic features of seven cases of bowen's disease (bd) of the nail bed, evaluates the role of human papillomavirus in the bowenoid change, and discusses optimal therapy. | 1994 | 8304759 |
| significance of human papillomavirus-induced squamous cell carcinoma to dermatologists. | 1994 | 8304764 | |
| cationic liposomes mediated delivery of antisense oligonucleotides targeted to hpv 16 e7 mrna in caski cells. | the "high risk" types 16 and 18 of human papillomavirus (hpv) are involved in the etiology of genital squamous cell carcinoma. the early genes 6 and 7 (e6-e7) of these viruses code for the major transforming proteins, capable of inducing cell transformation alone or acting synergistically with other oncogenes. antisense oligonucleotides, recently applied to inhibit the functions of a number of cellular and viral proteins, might provide the basis for a new therapeutic strategy against hpv-induced ... | 1994 | 8147581 |
| sequence variation in the capsid protein genes of human papillomavirus type 16. | we have cloned and sequenced the l1 and l2 genes from human papillomavirus type 16 (hpv16) dna-containing cervical cytology samples collected from the u.k. and trinidad. samples containing high copy numbers of hpv16 dna were selected as being likely to contain fully functional virus dna molecules in an episomal state, rather than in an integrated and possibly altered state. in comparison with the previously published sequence of hpv16 isolated from an invasive cancer a variety of differences wer ... | 1994 | 8151305 |
| [analysis of p53 gene in gynecologic tumors]. | the inactivation of the tumor suppressor gene p53 has been demonstrated in a variety of human tumors. herein, we performed a p53 gene analysis of human gynecologic tumor cell lines and tumor tissues. in the sk-ov-3 cell line, southern analysis suggested the presence of sequence deletions/rearrangements in at least one allele of p53 gene. transcripts were not detectable by either northern or pcr analysis. sequencing analysis of the entire coding region revealed mutations changing the p53 amino ac ... | 1994 | 8152158 |
| local epidermal viral infections: comparative aspects of vaccinia virus, herpes simplex virus and human papillomavirus in man and orf virus in sheep. | 1994 | 8156048 | |
| human cytotoxic t lymphocytes stimulated by endogenously processed human papillomavirus type 11 e7 recognize a peptide containing a hla-a2 (a*0201) motif. | cytotoxic t lymphocytes (ctl) may play an important role in the control of human papillomavirus (hpv)-induced anogenital neoplasias, but have been difficult to study owing to the difficulty in obtaining sufficient quantities of infectious virus. to address this we have stimulated human hpv-specific ctl in vitro using low-density cells (ldc) from peripheral blood mononuclear cells (pbmc). low-density cells were used to present synthetic peptides, or endogenously processed peptides expressed from ... | 1994 | 8157270 |
| detection of human papillomaviruses in paraffin-embedded biopsies of cervical intraepithelial lesions: analysis by immunohistochemistry, in situ hybridization, and the polymerase chain reaction. | one hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (cil) were tested for the presence of human papillomavirus (hpv) by immunohistochemistry and in situ hybridization using commercial biotinylated probes (vira-type in situ assay; digene diagnostics, silver spring, md) or probes labeled with digoxigenin by the random primer technique. immunohistochemistry was more inferior to the in situ hybridization method, with a detection rate of 14% (20/140) compared t ... | 1994 | 8159640 |
| absence of human papilloma virus dna in oropharyngeal spindle-cell squamous carcinomas. | the presence of human papilloma virus (hpv) dna in verrucous carcinomas and some squamous-cell carcinomas (scc) of the oropharynx suggests a role for the virus in their pathogenesis. spindle-cell squamous carcinoma is an uncommon variant of oropharyngeal scc. its association with the hpv has not been reported. in situ and dot blot hybridization, using biotinylated probes to hpv 6, 11, 16, 18, 31, 33, and 35, failed to detect hpv dna in nine studied cases of spindle-cell squamous carcinoma, as di ... | 1994 | 8160645 |
| human papillomavirus and endocervical adenocarcinoma. | 1994 | 8119727 | |
| risk of genital human papillomavirus infection in women with human immunodeficiency virus-induced immunosuppression. | this study examined the risk factors for genital human papillomavirus (hpv) infection, including hiv-related immunosuppression as well as demographic and behavioral factors. subjects were 207 women with known hiv serologic status, the majority of whom were intravenous drug users recruited from a methadone treatment program in the bronx, new york. a structured questionnaire interview, cervico-vaginal lavage, and cd4 cell measurements were obtained. hpv genomes in the cervico-vaginal cells were de ... | 1994 | 8119767 |
| the prevalence of cervical infection with human papillomaviruses and cervical dysplasia in alaska native women. | alaska native women historically have high rates of sexually transmitted diseases (stds) and invasive cervical cancer. their prevalence of cervical infections with human papillomavirus (hpv) in relation to cervical dysplasia was determined with a commercial dot hybridization test for seven hpv genotypes. type-specific hpv dna, similarly distributed between genotype groups 16/18 and 31/33/35, was detected in 234 cervical specimens (21%) from 1126 alaska native women seeking routine care and colpo ... | 1994 | 8133094 |
| a virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16. | previous studies have demonstrated that genital infection with high-risk types of human papillomavirus (hpv), most often hpv16, is the most significant risk factor for the development of cervical cancer. however, serologic assays that have been developed to identify high-risk hpv infection have either failed to associate serum reactivity with other indicators of hpv infection or have identified only a minority of hpv-infected individuals. | 1994 | 8133532 |
| tracheobronchial involvement of laryngeal papillomatosis at onset. | a case of adult onset laryngeal papillomas with tracheal and bronchial involvement present at onset is presented. the presence of human papilloma virus (hpv) dna type 6/11 is demonstrated by in situ hybridization. | 1994 | 8163923 |
| the absence of vaginal human papillomavirus 16 dna in women who have not experienced sexual intercourse. | to determine whether young women who have not experienced sexual intercourse may harbor genital human papillomavirus (hpv) infection in the vaginal-ectocervical mucosa. | 1994 | 8164934 |
| the state of the p53 gene in human papillomavirus (hpv)-positive and hpv-negative genital precancer lesions and carcinomas as determined by single-strand conformation polymorphism analysis and sequencing. | human papillomavirus (hpv) is frequently associated with cervical carcinoma. inactivation of the p53 tumor suppressor gene product by binding to the hpv encoded e6 protein is considered as an important pathway for malignant progress in hpv-infected cells. in contrast, mutations of the p53 gene have been found in hpv-negative cervical carcinoma cells. to evaluate the involvement of p53 inactivation for the development of genital carcinoma, we determined the state of the p53 gene in 20 genital pre ... | 1994 | 8166446 |
| human papillomavirus and epstein-barr virus in the etiology of testicular germ cell tumours. | epidemiological features suggest that the risk of testicular cancer may be related to exposure to unknown infectious agents, including viruses. therefore a series of twenty specimens of testicular germ cell tumours, including preinvasive carcinoma in-situ, were tested for the presence of dna sequences of two viruses with known transforming abilities, human papillomavirus (hpv) and epstein-barr virus (ebv). the polymerase chain reaction (pcr) technique was used. in none of the 19 successfully amp ... | 1994 | 8166998 |
| prevalence of anal human papillomavirus infection and intraepithelial neoplasia in renal allograft recipients. | a study was performed to test the hypothesis that renal allograft recipients are at high risk of developing anal human papillomavirus (hpv) infection and anal intraepithelial neoplasia (ain). a total of 133 renal allograft recipients and 145 control patients underwent anoscopy and biopsy. a polymerase chain reaction was used to detect hpv16 dna in biopsy samples. a histological diagnosis of anal hpv infection or ain was made in 32 allograft recipients (hpv infection, five; ain i, 20; ain ii, thr ... | 1994 | 8173899 |
| interferon and retinoic acid suppress the growth of human papillomavirus type 16 immortalized cervical epithelial cells, but only interferon suppresses the level of the human papillomavirus transforming oncogenes. | in the present study, we examine the effects of all-trans-retinoic acid (ra) and interferons-alpha and -gamma (ifn-alpha and ifn-gamma) on the growth of hpv16-immortalized cell lines, ece16-1 and caski. treating proliferating ece16-1 cells with ra causes a concentration-dependent decrease in cell number. at 1 microm ra, cell growth is suppressed by 65% and the level of mrna encoding cytokeratin k5, a biochemical marker of retinoid action, is also suppressed. in contrast, the level of transcript ... | 1994 | 8174114 |
| analysis of hpv-positive and -negative vulvar carcinomas for alterations in c-myc, ha-, ki-, and n-ras genes. | mutation or overexpression of certain host genes, including c-myc, ha-ras, and ki-ras, have been associated with genital squamous neoplasia, specifically in the cervix, and have been implicated in the natural history of these tumors. the relationship of these host gene alterations to vulvar squamous cell carcinomas has not been previously studied. we analyzed archival material from 13 human papillomavirus-positive and -negative vulvar squamous cell carcinomas for mutations in ha-, ki-, and n-ras ... | 1994 | 8175026 |
| fast solid support detection of human papillomavirus in in vitro amplified dna using a dnp-anti dnp monoclonal antibody couple. | in some anogenital lesions the detection of certain types of human papilloma virus, especially oncogenic types, is of interest. in a first step during a prospective study, we compared two methods for the detection of human papillomavirus (hpv) dna in clinical samples: southern blotting followed by hybridization with a cloned radioactive genomic probe and a classical polymerase chain reaction (pcr) followed by hybridization with a 32p-labelled oligonucleotide probe. 118 biopsies and swabs were ex ... | 1994 | 8175945 |
| stomach cancer in transgenic mice expressing human papillomavirus type 16 early region genes from a keratin promoter. | certain human papillomaviruses (hpvs) have been implicated as important contributory factors in the development of cervical carcinoma and other epithelial malignancies. in order to investigate the role of papillomavirus early gene expression in epithelial oncogenesis in vivo, we produced transgenic mice expressing hpv-16 early region genes from the promoter of the bovine keratin 6 gene. spliced transcripts were detected in the tongue, forestomach, glandular stomach, female reproductive tract and ... | 1994 | 8176373 |
| development of a broad spectrum pcr assay for papillomaviruses and its application in screening lung cancer biopsies. | a pcr assay was developed to detect known and as yet unidentified papillomaviruses (pvs). for this purpose we analysed the conserved amino acid sequences in the l1 and e1 open reading frames of 45 human and nine animal pvs. candidate regions for the design of a primer were identified as those having the least number of amino acid and nucleotide sequence variants among the different pvs. these regions in the l1 orf have been described previously. we modified the sequences of the backward and the ... | 1994 | 8176375 |
| assembly of the major and the minor capsid protein of human papillomavirus type 33 into virus-like particles and tubular structures in insect cells. | native virions of human papillomaviruses (hpv) can be isolated from genital lesions only in very limited amounts. recent studies have shown that virus-like particles can be obtained by expression of the capsid proteins using vaccinia virus recombinants or the baculovirus system. we now present the first detailed characterization of virus-like particles of a human papillomavirus associated with malignant genital lesions, hpv-33, produced in high yield using the baculovirus expression system. asse ... | 1994 | 8178438 |
| cervical neoplasia in the patient with hiv infection. | with heterosexual transmission of hiv becoming the primary mode of transmission to women in the usa, and a high rate of human papillomavirus (hpv) and cervical neoplasia in hiv-infected women, obstetrician-gynecologists have become primary care providers for hiv-infected women. reports of a high rate of recurrence and progression of cervical neoplasia in this population suggest that gynecologists must strive to identify those women with cervical neoplasia who are hiv infected. alterations in loc ... | 1994 | 8180357 |
| association between hla-dqb1 alleles and risk for cervical cancer in african-american women. | squamous-cell carcinoma of the cervix and its precursor lesions are associated with human papillomavirus (hpv) infection. epidemiological studies indicate that hpv infection in itself is not sufficient for cervical-cancer induction, suggesting that other factors contribute to carcinogenesis. we have investigated the potential role of host genetic background as one such factor. we screened a series of squamous-cell carcinomas of the cervix for hla-class-ii dqb1* alleles by the polymerase chain re ... | 1994 | 8181853 |
| a population-based seroepidemiological study of cervical cancer. | the epidemiology of cervical cancer indicates the presence of a sexually transmitted risk factor, attributable at least in part to infection with human papillomavirus (hpv) type 16 or 18. we performed a seroepidemiological study of hpv and cervical cancer in the counties of västerbotten and norrbotten in northern sweden, a low-risk area for cervical cancer. sera from 94 cases of incident cervical cancer were matched against 188 age- and sex-matched controls derived from a population-based blood ... | 1994 | 8261434 |
| adult rhabdomyoma. a light microscopic, ultrastructural, virologic, and immunologic analysis. | adult rhabdomyoma is a rare benign tumor of skeletal muscle origin that frequently arises from musculature of the head and neck region. in this study we report five cases of adult rhabdomyoma. light and electron microscopic examination revealed the typical cellular features as described by others. the results of immunophenotyping and quantitation of nucleolar organizer regions were consistent for a benign skeletal muscle tumor. epstein-barr virus and human papillomavirus genomic dna could not be ... | 1994 | 8139832 |
| laser treatment of urethral condyloma: a five-year experience. | twenty cases of men treated with the neodymium:yttrium-aluminum-garnet (nd:yag) laser for meatal and/or urethral condyloma over five years were reviewed. | 1994 | 8284887 |
| detection of human papillomavirus type 16 e6/e7 transcripts in histologically cancer-free pelvic lymph nodes of patients with cervical carcinoma. | human papillomavirus (hpv) 16 e6/e7 mrnas have been detected in paraffin-embedded sections of histologically cancer-free pelvic lymph nodes from four of six patients with hpv 16-associated cervical cancer. the cdna obtained from the viral mrna by reverse transcription was amplified by the polymerase chain reaction (pcr). transcripts were present in a small but significant proportion (6/42, 14%) of the histologically negative/hpv 16 dna-positive lymph node blocks; but neither hpv 16 dna nor trans ... | 1994 | 8314141 |
| vulvar squamous cell carcinoma and papillomaviruses: indications for two different etiologies. | the vulvectomy specimens of 78 patients with vulvar squamous cell carcinoma were reviewed and examined for human papillomavirus (hpv) types 6, 11, 16, 18, and 33 by the polymerase chain reaction technique. the tumors were classified as keratinizing squamous cell carcinoma (ksc), as warty carcinoma (wc), and as basaloid carcinoma (bc). dna of hpv types 16 and 33 was found in 2/51 ksc, in 12/17 wc, and in 10/10 bc. hpv types 6, 11, and 18 were not detected. patients with wc and bc were younger, an ... | 1994 | 8314146 |
| parathyroid hormone-related protein and human papillomavirus in gynecological tumors. | the presence of parathyroid hormone-related protein (pthrp) and human papillomavirus (hpv) in a series of gynecological tumors from 131 unselected patients was examined. pthrp was localized immunohistochemically using a highly specific rabbit polyclonal anti-serum against pthrp(1-16). the results confirmed that gynecological malignancies, although rarely associated with humoral hypercalcemia of malignancy (hhm), stained for pthrp in a majority of the squamous-cell carcinomas (scc) at all sites, ... | 1994 | 8314318 |
| effect of actinomycin-d on the cell-cycle progression and the expression of p53, waf1/cip1, gadd45, and mdm-2 genes in human oral keratinocytes - implication of human papillomavirus infection. | exposure of human oral keratinocytes immortalized by transfection with 'high risk' hpv dna to chemical carcinogens converts the cells to malignant phenotype, but it does not transform normal cells. to investigate the underlying mechanism for different chemical carcinogen susceptibility of normal and the hpv-immortalized oral keratinocytes to genotoxic agent, we studied the progression of cell cycle and the expression of p53, waf1/cip1, gadd45 and mdm-2 genes in normal and in the hpv-immortalized ... | 1994 | 21559675 |
| current status of prostate-cancer research - development of in-vitro model systems (review). | prostate cancer is a major medical problem which is expected to affect over 200,000 us men in 1994. despite its widespread prevalence and the difficulties in clinical diagnosis and treatment of the disease, the contribution of environmental factors and the etiological mechanism of prostate cancer are poorly understood. a brief update on recent progress in cellular and molecular research of prostate cancer is provided. specific areas discussed include oncogenes (vas, myc, c-erbb-2 and bcl-2), tum ... | 1994 | 21559703 |
| interrelationship between the expression of h-ras p21, c-myc and p53 proteins, and the infection of high-risk human papillomaviruses in japanese patients with esophageal squamous-cell carcinoma. | the presence and distribution of increased expression of h-ras p21 protein, c-myc protein and p53 protein, or of human papillomavirus (hpv) dna were investigated in 42 japanese patients with esophageal squamous cell carcinoma (scc) by immunohistochemical techniques using each protein antibody, and by in situ hybridization with fluorescein isothiocyanate (fitc)-labelled dna. probes for hpv-16 and hpv-18. eighteen cases were positive with h-ras p21 antibody, but only one with c-myc protein antibod ... | 1994 | 21607450 |
| breast and cervical cancer screening of poor, elderly, black women: clinical results and implications. harlem study team. | our objective is to describe the clinical findings from a nurse-practitioner-based breast and cervical cancer screening program for poor, elderly, black women. we designed a cross-sectional descriptive study set at an urban public hospital medical clinic. all women 65 years of age and older were eligible to be screened. we measured these main outcomes: rates of participation, abnormal tests, and neoplasia. women were offered screening during a routine visit. of 689 women, 491 (71%) participated. ... | 1993 | 8347363 |
| interleukin-6 (il-6) functions as an autocrine growth factor in cervical carcinomas in vitro. | many factors have been implicated in the etiology of cervical neoplasia, with human papilloma virus being the latest in a long line of agents that may on their own or in combination exert various initiating and promoting effects on cervical cells, resulting in their transformation. however, for such altered cells to become invasive, it is clear that they must undergo alterations in their rate of turnover, state of differentiation, and motility. we have investigated the production of the multifun ... | 1993 | 8349158 |
| anal dysplasia in homosexual men: role of anoscopy and biopsy. | both anal squamous cell cancer and human papilloma virus (hpv) are increased in homosexual men. this study evaluates histology of internal anal abnormalities in a high-risk population of human immunodeficiency virus (hiv) seropositive and seronegative homosexual men. | 1993 | 8359638 |
| sv40 t antigen abrogates p53-mediated transcriptional activity. | recent evidence suggests that the tumor-suppressor protein p53 functions as a transcriptional regulator to control cell proliferation. an interaction with p53 is required for sv40 t antigen to transform primary cells; however, the effect of t antigen binding on p53 function is not known. in order to determine if an interaction with t antigen results in loss of p53-mediated transcriptional activity, we have used vectors expressing either a p53-gal4 fusion protein or a wild-type p53 protein in tra ... | 1993 | 8378089 |
| ras gene mutations and hpv infection are common in human laryngeal carcinoma. | to evaluate the role of ras activation and human papillomavirus (hpv) infection in laryngeal carcinoma, we analyzed tumor dna from 43 cases, including 25 primary laryngeal tumors, 12 lymph-node and one skin metastases, and 5 recurrent laryngeal carcinomas. thirteen normal laryngeal tissues and 7 benign laryngeal nodule biopsy specimens along with normal tissue surrounding laryngeal carcinoma in 2 cases were also included. the polymerase-chain-reaction technique was used to amplify dna fragments ... | 1993 | 8380055 |
| human papillomavirus type 16 expression in cervical keratinocytes: role of progesterone and glucocorticoid hormones. | to determine the role of the steroid hormones, progesterone and glucocorticoids, and the viral hormone response elements, in the episomal expression of human papillomavirus (hpv) type 16 in primary human ectocervical cells. | 1993 | 8380104 |
| penile intraepithelial neoplasia in patients examined for exposure to human papilloma virus. | female genital malignancies have recently been associated with infection with various types of human papilloma virus. we present 9 cases of penile intraepithelial neoplasia found in men examined for exposure to human papilloma virus. six of these men had acetowhite lesions and underwent biopsies to confirm the potential presence of condylomata acuminata. the remaining 3 patients had lesions that were visible but whose nature was in doubt. in addition to condylomata acuminata, penile intraepithel ... | 1993 | 8380511 |
| correlation between hpv positivity and state of the p53 gene in cervical carcinoma cell lines. | human papillomavirus (hpv) infection is clearly associated with cervical carcinomas, yet it is also true that there are cervical carcinomas in which hpv dna is absent. we examined eight established cell lines derived from cervical carcinomas for the presence of mutations of the p53 antioncogene in relation to the presence of hpv dna sequences. of these eight cell lines, seven were positive for hpv dna and the remaining one was negative for hpv dna. single-strand conformation polymorphism analyse ... | 1993 | 8380785 |
| human papillomavirus dna in distant metastases of cervical cancer. | eighteen distant metastases from cervical cancer to the extrapelvic abdomen, extraabdominal lymph nodes, vulva, suburethral region, skin, and breast in 17 patients were analyzed by southern blot hybridization under nonstringent and stringent conditions for the prevalence of human papillomavirus (hpv) type 11, 16, 18, 31, 33, and 35 dna. fourteen metastases in thirteen patients were hpv-positive. thirteen tumors contained hpv-16 and one hpv-related sequences with varying copy number. in 9 of 11 c ... | 1993 | 8380788 |
| hpv16 e7 protein associates with the protein kinase p33cdk2 and cyclin a. | e7 is the major transforming protein of human papillomavirus type 16 (hpv16). it has been found to associate with the retinoblastoma protein rb1. we investigated whether hpv16 e7 protein was associated with other cellular proteins, in particular with those involved in cell cycle control. immunoprecipitates from caski cell extracts with an anti e7 monoclonal antibody contained a histone h1 kinase. recombinant e7, synthesized in yeast, when mixed with protein extracts from epithelial cells bound h ... | 1993 | 8380917 |
| retinoic acid inhibition of human papillomavirus type 16-mediated transformation of human keratinocytes. | we previously reported that human keratinocytes (hkc) immortalized by transfection with human papillomavirus type 16 dna (hkc/hpv16) are more sensitive than normal hkc to growth inhibition by retinoic acid (ra), and that ra treatment of hkc/hpv16 inhibits hpv16 e6/e7 mrna expression (l. pirisi et al., cancer res., 52: 187-193, 1992). we now demonstrate that hpv16 e2 and e5 mrnas are also decreased by ra treatment of hkc/hpv16, indicating a general inhibition by ra on the expression of hpv16 earl ... | 1993 | 8381332 |
| immunoelectron microscopical localization of human papillomavirus type 16 l1 and e4 proteins in cervical keratinocytes cultured in vivo. | the human papillomavirus (hpv) causes warts, but is also associated with the development of squamous cell dysplasia and carcinoma. the virus is host and tissue specific and the numerous hpv types show predilection for different body sites. experimental production of hpv 16 particles is at present only possible using in vivo culture of keratinocytes containing episomal viral dna. using immunoelectron microscopy, we have investigated the localization of hpv 16 e4 and l1 proteins in a keratinized e ... | 1993 | 8381449 |
| antibodies against papillomavirus antigens in cervical secretions from condyloma patients. | samples of cervical secretions and serum from 30 women with genital condylomas and 30 age-matched controls were tested for the presence of immunoglobulin a (iga) and igg antibodies against a panel of papillomavirus-derived antigens. the same cervical samples were also analyzed for presence of human papillomavirus (hpv) dna by southern blotting and polymerase chain reaction. by southern blotting hpv dna was detected in 8 of 30 patients with condylomas and 2 of 30 controls, and by the polymerase c ... | 1993 | 8381807 |
| serological response to hpv 16 in cervical dysplasia and neoplasia: correlation of antibodies to e6 with cervical cancer. | sera from patients with cervical cancer, cervical intraepithelial neoplasia (cin) and non-genital cancers, and from healthy individuals, were investigated for antibodies to human papilloma virus (hpv) early proteins e4, e6 and e7 and the major capsid protein li by western blot analysis of recombinant hpv proteins. there was a significantly higher prevalence of sera with antibodies to e6 in cervical cancer patients than in healthy individuals or in cin or non-genital-cancer patients. antibodies t ... | 1993 | 8382193 |
| genetic analysis of in vitro progression of human papillomavirus-transfected human cervical cells. | the authors have established an in vitro model system which demonstrates the progression of the transformed phenotypes of human cervical epithelial cells transfected with human papillomavirus (hpv) type 16 and 18 dnas. both viral dnas exhibit immortalizing potential; however, only hpv 18-immortalized cell lines progress to exhibit anchorage-independent growth and, in a limited number of cases, tumorigenesis. in this paper, the authors have examined the genetic basis for this in vitro progression ... | 1993 | 8382557 |
| comparison between methods for human papillomavirus dna testing: a model for self-testing in young women. | this study examined the accuracy of samples obtained by women with dacron swabs for testing for human papillomavirus (hpv). a total of 114 women obtained samples with the swabs and then had routine pelvic examinations done by nurse practitioners during which endocervical and exocervical samples were obtained by speculum-assisted technique. these samples were used for hpv dna testing and cytology. the results of both sets of samples were compared. a total of 104 samples (91%) obtained by self-sam ... | 1993 | 8382720 |
| oral cancer and human papillomaviruses: is there a link? | cancer of the oral cavity accounts for almost 56,000 new cases in india each year totalling almost 30% of all cancer cases in the country. despite the numerous advances in etiology and epidemiology, the mechanisms involved in oral carcinogenesis remain obscure. current research has provided some provocative results, suggesting an association between human papillomavirus (hpv) and development of squamous cell tumors including oral cancer. evidence has been presented showing hpv infection of the o ... | 1993 | 8382758 |
| genital hpv infection not a local but a regional infection: experience from a female teenage group. | to investigate the prevalence of human papillomavirus (hpv) infections in a group of female teenagers, and to analyse to what extent hpv dna was also detectable, in urethra and cervix samples among the patients with macroscopic genital warts compared with those without. | 1993 | 8383095 |
| intramuscular beta-interferon treatment for severe vulvar vestibulitis. | seven patients with vulvar vestibulitis associated with human papillomavirus (hpv) infection were treated with intramuscular (im) injections of human beta-interferon. the im route was chosen to avoid painful intralesional injections. complete remission of symptoms was achieved in three patients who received 45 x 10(6) iu of beta-interferon; they remained asymptomatic for a duration of 6-18 months following treatment. of the four patients who received only 30 x 10(6) iu, two had complete remissio ... | 1993 | 8383204 |
| development of type-specific and cross-reactive serological probes for the minor capsid protein of human papillomavirus type 33. | human papillomavirus type 33 (hpv33) is associated with malignant tumors of the cervix. in an attempt to develop immunological probes for hpv33 infections, antisera against various bacterial fusion proteins carrying sequences of the minor capsid protein encoded by l2 were raised in animals. antigenic determinants on the hpv33 l2 protein were identified by using truncated fusion proteins and were classified as type specific or cross-reactive with respect to hpv1, -8, -11, -16, and -18. cross-reac ... | 1993 | 8383218 |