Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| nonresolution of an amebic liver abscess after parasitologic cure. | a 43-year-old filipino male was admitted to a manila hospital with a 1 month history of epigastric pain and fever, and was found to have a palpable epigastric mass. computerized tomography revealed a large hepatic abscess which serologically was shown to be amebic. chemotherapy resulted in clinical cure and an initial reduction in size of the liver abscess. however, resolution of the abscess cavity did not occur, and on closed needle aspiration, 80 cc of characteristic amebic pus was recovered. ... | 1986 | 2871768 |
| fulminant amebic colitis. | nine patients with fulminating amebic colitis who were treated surgically from 1975 to 1982 are presented. only those who had bowel resections with exteriorization of the cut ends survived. the pertinent literature is reviewed briefly. | 1986 | 2872021 |
| specific circulating immune complexes in amoebic liver abscess. | a micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (cic). a cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. among the patients with amoebic liver abscess, 93% (14 of 15 confirmed cases) had indications of amoebic antigen in cic. in 57% of the suspected cases of amoebic liver abscess, amoebic antigen could be detected in cic. the specifici ... | 1986 | 2872230 |
| the interaction of human neutrophils and entamoeba histolytica increases cytopathogenicity for liver cell monolayers. | the in vitro interaction of neutrophils and virulent axenic entamoeba histolytica trophozoites on chang liver cells was studied to determine if the presence of neutrophils enhanced destruction of liver cell monolayers. axenic amoebae (strain hm1:imss) destroy liver cell monolayers in a dose-dependent manner (p less than .001). human neutrophils had no effect on the liver cell monolayers; however, when neutrophils were added to amoebae, destruction of monolayers was enhanced (p less than .05 comp ... | 1986 | 2872253 |
| isolation and characterization of phagocytosis- and virulence-deficient mutants of entamoeba histolytica. | we have used chemical mutagenesis to isolate 10 independent phagocytosis-deficient mutants of entamoeba histolytica from a highly virulent clone (strain hm1:imss). phagocytosis-deficient mutants were characterized by several biologic properties related to amoebic pathogenic mechanisms: virulence, adhesion to red blood cells, cytopathic effect of live trophozoites on cell culture monolayers, ability of amoebic extracts to destroy cell culture monolayers, and growth in semisolid agar. all of these ... | 1986 | 2872254 |
| purine-metabolising enzymes in entamoeba histolytica. | the enzymes that catalyse the salvage of purines in entamoeba histolytica trophozoites have been surveyed. adenine deaminase (ec 3.5.4.2), adenosine deaminase (ec 3.5.4.4), guanine deaminase (ec 3.5.4.3), adenine phosphoribosyltransferase (prtase) (ec 2.4.2.7), xanthine prtase (ec 2.4.2.22) and hypoxanthine prtase (ec 2.4.2.8) were all detected in cell homogenates but only at low activities, whereas amp deaminase (ec 3.5.4.6) and guanine prtase (ec 2.4.2.8) were not found. phosphorylases (ec 2.4 ... | 1986 | 2872591 |
| non-ulcerative amebiasis of rectum. | 1986 | 2872792 | |
| entamoeba histolytica: effect of growth conditions and bacterial associates on isoenzyme patterns and virulence. | in xenic culture, isolates of entamoeba histolytica from asymptomatic carriers are characterized, with rare exception, by possession of a nonpathogenic zymodeme. during the process of axenizing such an isolate, strain cdc:0784:4, a change in the pattern of the isoenzymes from nonpathogenic zymodeme i to pathogenic zymodeme ii was observed 40 days after the amebae had been transferred from a medium for xenic cultivation to one used for axenic cultivation, but before axenization of the amebae had ... | 1986 | 2873049 |
| production of mouse monoclonal antibodies which inhibit in vitro adherence of entamoeba histolytica trophozoites. | adherence by axenic entamoeba histolytica trophozoites to mammalian cells is mediated by an n-acetylgalactosamine (galnac)-inhibitable adhesin on the surface of the parasite. we isolated 35 hybridoma cell lines producing antibodies to e. histolytica as indicated by elisa with sonicated amebic protein or by immunofluorescence assay with fixed whole trophozoites. tissue culture supernatants were further screened for subcloning by the ability to bind to chinese hamster ovary (cho) cells which were ... | 1986 | 2873102 |
| the effect of colonic bilharzial lesions on the prevalence and invasiveness of intestinal amoebiasis. | 1986 | 2873192 | |
| evaluation of different cultures used for cultivation of entamoeba histolytica in arab republic of egypt. | 1986 | 2873193 | |
| ionophore a23187 stimulates entamoeba histolytica to release prostaglandin f2 alpha. | high performance liquid chromatography and radioimmunoassay were used to identify the prostaglandins synthesized by entamoeba histolytica (nih:200 strain). ca2+ionophore a 23187 stimulated amebae produced elevated levels of prostaglandin f2 alpha when compared with the control cells. there were no significant differences in the levels of prostaglandin e2 and thromboxane b2. this is the first report of a stimulated non-mammalian cell producing prostaglandin f2 alpha. | 1986 | 2873586 |
| [incidence of entamoeba histolytica in children with chronic diarrhea without macroscopic evidence of blood in the feces]. | 1986 | 2873654 | |
| in vitro activity of a substituted phenanthroline on entamoeba histolytica. | 1986 | 2873667 | |
| comparative in vitro activity of mefloquine, diloxanide furoate and other conventionally used amoebicides against entamoeba histolytica. | 1986 | 2874011 | |
| loss of susceptibility to complement lysis in entamoeba histolytica hm1 by treatment with human serum. | entamoeba histolytica hm1, exposed to a series of treatment with normal human serum (nhs), progressively lost susceptibility to complement lysis. trophozoites were incubated daily with unheated or heat-inactivated nhs for 2 hr at 36 degrees, starting with 10% v/v serum and increasing by 5% every 3 days up to 40% nhs. resistance to complement lysis was also obtained with two different hm1 clones but not with the low virulent strain hk9. induction of resistance dependent on the number of nhs treat ... | 1986 | 2874111 |
| antibody-dependent macrophage-mediated cytotoxicity against entamoeba histolytica. | interactions between trophozoites of entamoeba histolytica and peritoneal exudate macrophages from unsensitised and antigen-sensitised animals were studied in vitro. normal macrophages killed trophozoites to some extent. this killing capacity was enhanced by prior sensitisation of the animals with specific antigen. incorporation of anti-amoebic antiserum in the amoeba-macrophage mixture greatly enhanced the killing capacity of macrophages. fraction one (f-i) of a crude amoebic extract was most e ... | 1986 | 2874228 |
| amoebic abscess of spleen. | 1986 | 2874240 | |
| entamoeba histolytica as a commensal intestinal parasite in homosexual men. | entamoeba histolytica is considered to be an uncommon, imported organism in the united kingdom and in many parts of north america, but recent attention has been drawn to the possibility of sexual transmission of this parasite among homosexual men. to determine the prevalence and clinical importance of enteric parasitic infections in men attending a clinic in london for the treatment of sexually transmitted diseases, we studied 354 randomly selected patients who provided a single stool sample tha ... | 1986 | 2874484 |
| isoenzyme patterns and pathogenicity in amebic infection. | 1986 | 2874486 | |
| treatment of homosexual men infected with entamoeba histolytica. | 1986 | 2874487 | |
| amebic infections in asymptomatic homosexual men, lack of evidence of invasive disease. | a survey for enteric infections in 140 asymptomatic homosexual men who attended a community clinic revealed a high prevalence of infection with entamoeba histolytica (27.1 per cent) and giardia lamblia (15.7 per cent). in contrast, the prevalence of elevated indirect hemagglutination (iha) titers (greater than or equal to 1:128), which indicate invasive amebiasis, was low (5.7 per cent). our findings suggest that only a limited amount of invasive amebic disease is occurring in this group of homo ... | 1986 | 2874747 |
| elucidation of cellular population and nature of anti-amoebic antibodies in cytotoxicity to entamoeba histolytica (nih:200). | interactions between trophozoites of entamoeba histolytica and cells of the immune system were studied in vitro by mixing effector cells obtained from normal and immunized guinea pigs with trophozoites in a ratio of 10:1. crude amoebic extract (cae) and its highest molecular weight (mw 650,000) fraction (f-i) were used for priming the effector cells in vivo. the effector cells were collected from the spleens of normal and immunized animals. lymphocytes were prepared by allowing splenic mononucle ... | 1986 | 2875144 |
| analysis of antigens from membrane and soluble fractions of entamoeba histolytica. | axenic cultures of entamoeba histolytica strains hk-9, hm-1, and rahman were fractionated to provide plasma membranes, internal, vesiculated membranes, and a soluble cytosol. each particulate fraction was solubilized and all fractions were analyzed by techniques designed to demonstrate molecular complexity and serologic reactivity. the cytosol contained more antigenic moieties than either membrane fraction; however, the antigens associated with the membranes had very high reactivity and lower no ... | 1986 | 2875181 |
| [research on helminth eggs and protozoan cysts on money]. | 1986 | 2875514 | |
| [enteropathogens in travellers returning from the tropics]. | diarrhea is not only the most common health hazard during travel in the tropics but also the most frequent condition which prompts returning travellers to see a physician. the prevalence of bacterial and parasitic enteropathogens in people attending our outpatient department after returning from the tropics has been studied and the laboratory results of stool examinations have been compared with clinical symptoms. of the 173 persons enrolled, 19 (11%) harboured bacterial pathogens and pathogenic ... | 1986 | 2875520 |
| in vitro amoebicidal testing of natural products; part i. methodology. | 1986 | 2876447 | |
| the detection of enteropathogens in acute diarrhea in a family cohort population in rural egypt. | in 8 villages of rural northeastern egypt, a 2-year study of the etiologic agents associated with episodes of diarrhea was carried out. stool specimens (3,243) from 3,513 episodes of diarrhea were processed for enteropathogens. the most commonly identified agents in the group with diarrhea were giardia lamblia (44%), heat stable enterotoxin (st)-producing enterotoxigenic escherichia coli (etec) (15%), heat labile toxin (lt)-producing etec (12%), enteropathogenic e. coli (epec) (4%), rotavirus (3 ... | 1986 | 2876656 |
| [measurement of oxidation-reduction potentials in the cultivation of entamoeba histolytica]. | 1986 | 2876762 | |
| in vitro antimicrobial effects of furazolidone & metronidazole. | 1986 | 2876955 | |
| prevalence of enteric parasites in homosexual patients attending an outpatient clinic. | a total of 372 pooled stool specimens from 274 homosexual men with diarrhea were submitted for parasitologic examination over a 2.5-year period. each two-vial pooled specimen set contained portions of stool from 3 consecutive days in formalin and polyvinyl alcohol. of the 274 patients, 133 (48.5%) harbored one or more intestinal protozoa, with 161 (43.3%) of the 372 specimens submitted being positive for one or more organisms. the parasites identified included entamoeba histolytica (71 patients) ... | 1986 | 2877006 |
| on three stages of amoebic research. | 1986 | 2877277 | |
| interaction of serum components with the cytotoxic action of entamoeba histolytica. | native normal human serum is capable of inhibiting the cytotoxic action of entamoeba histolytica against k562 tissue culture target cells assessed by a 51cr-release test. it is suggested that a part of the inhibitory activity on amoebae's cytotoxic action is represented by the complement system which is known to lyze trophozoites by activation of the alternative pathway. for the rest of the serum's inhibitory activity on amoebic cytotoxic action molecule(s) is (are) responsible which act(s) inde ... | 1986 | 2877551 |
| factors contributing to the pathogenic behavior of entamoeba histolytica. | 1986 | 2877612 | |
| impaired production of alpha and gamma interferon in asymptomatic homosexual males. | in vitro production of alpha interferon and gamma interferon was examined in cell cultures from 90 asymptomatic homosexual males and 19 healthy heterosexual male controls. the production of alpha and gamma interferon was significantly suppressed in homosexuals as compared to that in heterosexual controls (p less than 0.005 and p less than 0.001, respectively). forty-one of the homosexuals produced less gamma interferon than any of the heterosexual controls. antibodies against the human immune sy ... | 1986 | 2877877 |
| changes in isoenzyme patterns of a cloned culture of nonpathogenic entamoeba histolytica during axenization. | the axenization of an entamoeba histolytica isolate with a nonpathogenic isoenzyme electrophoretic pattern (zymodeme) was recently achieved for the first time (15). forty days after the cells were transferred to the medium used for axenic cultivation, the amebae developed virulence properties, and the zymodeme converted to a pathogenic pattern. to exclude the possibility that the original isolate consisted of two zymodeme populations and that conditions of growth selected for a particular popula ... | 1986 | 2877951 |
| amoeba research and history. | 1986 | 2878270 | |
| macrophage spreading inhibition test as an alternate method for in vitro assessment of cell-mediated immunity against entamoeba histolytica. | during cellular immune responses sensitized lymphocytes release a number of mediators collectively known as 'lymphokines'. the assaying of macrophage spreading inhibition (msi) factor released by sensitized lymphocytes was employed as an alternative procedure for in vitro detection of cell-mediated immunity (cmi) in animals immunized with amoeba antigen preparation. the msi test was compared with macrophage migration inhibition test. a well defined cmi was detectable in animals immunized by amoe ... | 1986 | 2878487 |
| immunodiagnosis of entamoeba histolytica and ascaris lumbricoides infections in caucasian and aboriginal australians. | the immunodiagnostic efficiency of an indirect immunofluorescence test (ifat) and in vitro lymphocyte proliferative responsiveness (cell mediated immunity test, cmit) used to measure the immunological responses of individuals with known natural entamoeba histolytica and ascaris lumbricoides infections, was studied under survey conditions. e. histolytica was common among aborigines from cherbourg, kowanyama and central australia, but it was not found in brisbane caucasians. the protozoan was sele ... | 1986 | 2878514 |
| isoenzyme patterns of entamoeba histolytica isolates from asymptomatic carriers: use of gradient acrylamide gels. | a vertical polyacrylamide gradient gel (3% to 7%) was designed to facilitate the electrophoretic resolution and classification of isoenzyme patterns of entamoeba histolytica isolates. the following enzyme systems were used: phosphoglucomutase (pgm), hexokinase (hx), glucosephosphate isomerase (gpi), and malate dehydrogenase (me). the modifications in the electrophoretic procedure and sample preparation allowed the reproducible comparison of enzyme patterns of axenic, monoxenic, and mixed culture ... | 1986 | 2878625 |
| longitudinal study of diarrhoeal disease in a peri-urban community in manaus (amazon-brazil). | a 20-month longitudinal study of diarrhoeal disease was carried out in a poor peri-urban community of manaus (amazon-brazil), and the attack rate of this disease ranged from 0.2 to 4.8 episodes of diarrhoea per person per year. the age group most affected was 0 to 35 months old. a probable aetiological agent was identified in 68 of the 110 faecal samples collected. the most frequent enteropathogens isolated were enterotoxigenic escherichia coli and giardia lamblia. enterotoxigenic escherichia co ... | 1986 | 2878647 |
| pathogens that cause travelers' diarrhea in latin america and africa. | with the advent of rapid and convenient means of transportation, millions of persons travel each year from industrialized to developing countries in the tropics and subtropics. these travelers are at risk for a variety of infectious diseases that are endemic in these areas; the most frequently occurring of these is diarrhea. studies of groups of travelers to latin america and africa have found that approximately one-half develop diarrhea during their stay abroad. etiologic investigations of thes ... | 1986 | 3014633 |
| intestinal helminthiasis and haemoparasitism in an area of endemic dracunculiasis in oyo state of nigeria. | in the course of a survey of human intestinal and haemoparasites in oluyole local government area of nigeria known to be endemic for dracunculiasis, most of the inhabitants were burdened with parasitic infections which include ascariasis (43.7%), hookworm infection (27.1%), trichuriasis (31%), strongyloidiasis (2.5%), entamoeba histolytica infestation (3.9%), e. coli infestation (2.7%) and plasmodiasis (43.7%). peak of ascariasis was among the 1-10 years age group while those of trichuriasis, ho ... | 1986 | 2944853 |
| cell-mediated immunity in amoebic liver abscess. | cell-mediated immunity studies were carried out in 26 cases of amoebic liver abscess by the leucocyte migration inhibition test and in 23 cases by t-cell counting. the tests were repeated after treatment and clinical recovery in 20 and 18 cases respectively. in addition, the leucocyte migration inhibition test was performed on 20 normal healthy controls and 11 entamoeba histolytica cyst passers, and t-cell counting was carried out on 18 normal healthy controls and nine e. histolytica passers. th ... | 1986 | 3491219 |
| prevalence of intestinal parasitic infections in riyadh district, saudi arabia. | a total of 23,516 stool specimens collected from patients attending three medical centres in riyadh, saudi arabia, were examined for intestinal parasites. of these 5737 (24.4%) were positive. entamoeba histolytica (8.8%) and giardia lamblia (6.3%) were the commonest parasites found, and in the age group of one to 15 years. giardia was found in 14.8% of males and 11.9% of females. other intestinal parasites present included ascaris lumbricoides, trichuris trichiura, schistosoma mansoni, hymeolepi ... | 1986 | 3675035 |
| intestinal parasitic infections in pre-school children in the niger delta. | stool specimens from 1,062 pre-school children in the niger delta were examined for the presence of intestinal parasites. of that number, 77.6% harboured one or more than one species of intestinal parasites. ascaris lumbricoides (51.9%) and trichuris trichiura (41.7%) were the most prevalent species recorded. other parasites harboured by these children included hymenolepis sp. (18.3%), giardia lamblia (9.2%), entamoeba histolytica (4.6%), strongyloides stercoralis (1.4%). hookworm, taenia sp., i ... | 1986 | 3701056 |
| amoebiasis--entamoeba histolytica infections: a review. | 1986 | 3709394 | |
| [intestinal parasitoses in children of an indian community of bolivian altiplano]. | a survey about motion parasitosis has been carried out on one hundred indians (quechua ethnic group) living in a hamlet called amarete located in the bolivian altiplano. this community is living in a place difficult to reach (11,100 feet) where many pre-colombian traditions are still alive. all children were parasitized, 75% of them were carrier of 3 to 5 parasites. infestation with ascaris (91%) is the most frequent and the risks of getting amebic dysentery (entamoeba histolytica 41%) and balan ... | 1986 | 3731367 |
| [a rare cause of 2 unclear fatalities: undiagnosed acute and chronic amebiasis]. | two cases are reported of unexpected and unclarified death caused by an infection with entamoeba histolytica. the first case concerned a german whose disease was not diagnosed during the asymptomatic phase (2 years' duration) or in the acute phase during the last 3 weeks before death. this is typical of the disease. in the final phase, he developed a hepatic abscess. death occurred as a consequence of bilateral apoplexia in the adrenal glands. in the second case a turk fell ill with gastralgia a ... | 1986 | 3765902 |
| blastocystis hominis: pathogen or fellow traveler? | blastocystis hominis, an intestinal organism of uncertain taxonomic position, has long been considered nonpathogenic. some recent studies suggest, however, that it may be associated with diarrhea and may respond to treatment with iodoquinol or metronidazole. to investigate this possibility, we identified 148 persons whose stools contained this organism. of this number, 32 had at least 6 stool examinations performed. twenty-seven of the 32 persons were later found to have greater than or equal to ... | 1986 | 3766850 |
| protozoal enteric infections among expatriates in bangladesh. | in order to study the prevalence, incidence, and symptoms of infections with giardia lamblia and entamoeba histolytica, we followed 251 expatriates in bangladesh over a 1-year period. microscopic examination of fecal specimens was performed upon enrollment, at 3-month intervals, and during episodes of diarrhea. specimens were cultured for bacteria and samples of serum and saliva were collected for antibody studies (igg and siga). the prevalence of g. lamblia infections was 5.2% and the incidence ... | 1986 | 3789269 |
| human amoebiasis: epidemiological studies at two hospitals in ibadan, nigeria. | investigations were conducted into the prevalence rates of entamoeba histolytica over a five year period in ibadan, qyo state of nigeria. between 1979 and 1983, 1465 (17.23%) patients out of 8499 people examined at the university of ibadan teaching hospital were positive for the infection. within the same period only 37 (0.88%) out of 4196 people examined at the adeoyo state hospital were infected. both male and female were affected. there is also no influence of age on the prevalence rates as b ... | 1986 | 3557832 |
| an 88,000-mr giardia lamblia surface protein which is immunogenic in humans. | human anti-giardia lamblia sera specifically immunoprecipitated an 88,000-mr surface protein from radioiodinated trophozoites, establishing this protein as a potentially important immunogen in humans. a mouse monoclonal antibody (gl-1) was isolated which immunoprecipitated the same 88,000-mr surface protein recognized by the human sera. gl-1 gave uniform fluorescent staining of the cell surface and flagella of g. lamblia trophozoites from the portland 1 and wb strains as well as fresh clinical i ... | 1986 | 3536744 |
| impairment of exercise tolerance due to broxyquinoline-brobenzoxaldine combination. | a case report of impaired exercise tolerance during administration of broxyquinoline-brobenzoxaldine combination in therapeutic doses is presented. the first author of this paper, a 28-year-old healthy male, volunteered as a subject for a prospective study on the neurotoxicity of halogenated hydroxyquinolines and took a course of broxyquinoline-brobenzoxaldine combination. the author, who is a karate enthusiast, observed impairment of exercise tolerance during the course of treatment with the dr ... | 1986 | 3081430 |
| parasitic infections in asymptomatic homosexual men: cost-effective screening. | the purpose of this study was to develop a cost-effective strategy for screening for enteric protozoan infections in homosexual men without gastrointestinal symptoms suggesting infection. one hundred and one homosexual men in portland, oregon, each submitted at least one unpurged stool sample; 91% submitted three samples each. of these, 27% had entamoeba histolytica, 61% had nonpathogenic protozoa with or without e. histolytica, 36% had a nonpathogen alone, and 3% had giardia lamblia. protozoan ... | 1986 | 3095515 |
| [intestinal parasitic colonization and protein-caloric malnutrition]. | intestinal parasitic infection was determined by fecal examination for parasites with several complementary methods in 380 young gabonese infants aged 6 to 24 months (190 with normal weights and 190 with malnutrition, matched for age). trichuris trichiura, ascaris lombricoides, giardia intestinalis, entamoeba histolytica and strongyloides stercoralis were the most prevalent parasites, without any difference between well-fed infants and patients with malnutrition. the higher prevalence of strongy ... | 1986 | 3101646 |
| a dot immunobinding assay (dia) on nitrocellulose membrane for the serological detection of antibodies to entamoeba histolytica. | a rapid, cheap, simple and specific serological test of adequate sensitivity for detecting igg antibodies against entamoeba histolytica antigen is described. axenically cultured amoebic antigen was used to precoat the nitrocellulose membrane. the strips were incubated with test samples and later with horseradish peroxidase (hrpo) labelled protein-a conjugate. a dark blue spot was obtained by treatment with peroxidase substrate, 4-chloro-1-naphthol, in positive samples. serum samples from 32 heal ... | 1986 | 2885949 |
| seroepidemiology of e. histolytica infection in hyderabad. | 1986 | 2885950 | |
| [isolation, characterization and in vitro translation of rna from clones of entamoeba histolytica deficient in adhesion]. | 1986 | 2439050 | |
| [axenic encystment of e. histolytica]. | 1986 | 2439051 | |
| wall synthesis in axenic cultures of entamoeba histolytica grown in pehps medium. | 1986 | 2439052 | |
| [role of histamine in the local anti-amebic immune response]. | 1986 | 2439053 | |
| studies on the substrate specificity of alpha- and beta-amylase of entamoeba histolytica. | from the supernatant fraction of cell homogenates of entamoeba histolytica alpha-amylase (1,4-alpha-d-glucan glucanohydrolase, ec 3.2.1.1) and beta-amylase (1,4-alpha-d-glucan maltohydrolase, ec 3.2.1.2) were separated and purified by gel filtration and isoelectric focusing, followed by deae- and cm-chromatography, respectively. both enzymes catalyzed the degradation of amylose, amylopectin and glycogen. hydrolysis of polysaccharides by alpha-amylase yielded as reaction products maltose, maltotr ... | 1986 | 2421162 |
| separation and characterization of four different amylases of entamoeba histolytica. i. purification and properties. | cell homogenate of entamoeba histolytica trophozoites was investigated for amylolytic activity against various biogenic and synthetic substrates. after gel filtration of the cell homogenate on sephadex g-150, six partly separated amylases (i to vi) differing in their substrate specificities were detected using maltose, amylose, amylopectin, 4-nitrophenyl alpha-glucoside and 4-nitrophenyl alpha-maltotetraoside. all enzymes are able to degrade amylose, amylopectin, glycogen and biogenic malto-olig ... | 1986 | 2423097 |
| separation and characterization of four different amylases of entamoeba histolytica. ii. characterization of amylases. | purified e. histolytica amylases iii to vi were characterized by their hydrolytic behaviour towards 4-nitrophenyl alpha-malto-oligosaccharides, malto-oligosaccharides, amylose, amylopectin, glycogen and y-cyclodextrin. the influence of specific inhibitors on the amylase activity of e. histolytica was examined and compared with typical alpha- and beta-amylases. amylases iii and iv showed alpha-glucosidase and glucosyltransferase activity by cleaving terminal non-reducing glucose from pnpg1 (iii, ... | 1986 | 2423098 |
| entamoeba histolytica: specific antigen recognized by a monoclonal antibody. | specific antigenic determinants on the membrane surface of entamoeba histolytica that distinguish it from other entamoeba species were demonstrated. evidence for these antigenic determinants was obtained with a monoclonal antibody to e. histolytica which showed not only specificity but also sensitivity as demonstrated in enzyme linked immunosorbent assay. immunofluorescence microscopy showed that the monoclonal antibody recognized an epitope present on the membrane surface of e. histolytica trop ... | 1986 | 2423362 |
| assessment of some preservatives and stains for entamoeba histolytica. | 1986 | 2431077 | |
| acid intracellular vesicles and the cytolysis of mammalian target cells by entamoeba histolytica trophozoites. | entamoeba histolytica kills mammalian target cells in a multi-step sequential process with separate adherence, cytolytic, and phagocytic events. in the studies reported here, we used fluorescein isothiocyanate linked to dextran to label the endocytic vesicles of the hm1 strain of e. histolytica and measure vesicle ph (5.1 +/- 0.2 by spectrofluorimetry). concentrations of nh4cl (1.0-10.0 mm) sufficient to increase vesicle ph to greater than or equal to 5.7 inhibited amebic killing of target chine ... | 1986 | 2432267 |
| immune consequences of amebiasis. | 1986 | 2418425 | |
| susceptibility of entamoeba histolytica to oxidants. | the effect of hydrogen peroxide and hypochlorite on culture forms of entamoeba histolytica trophozoites was examined by using two strains of e. histolytica, virulent (ip:0682:1) and nonvirulent (dkb). the amoebae were incubated with various concentrations of hydrogen peroxide and hypochlorite, and their viability was determined at different times after incubation. when the viability of the virulent and nonvirulent strains was compared to different oxidant strengths, it became apparent that the v ... | 1986 | 2867045 |
| effects of amiloride on na+ content and pinocytosis in entamoeba histolytica. | amiloride, a blocker of na+ leak and na+-h+ exchange in animal cells, caused cells of entamoeba histolytica to release na+ (up to 40% of their original na+ content within 90 min, at an amiloride concentration of 3 mm); k+ content was not affected. by comparing the unidirectional uptake of 22na+ with that of the fluid-phase marker 125i-labeled poly(vinylpyrrolidone) we established that the amiloride-induced na+ loss was due to inhibition of pinocytic na+ uptake rather than to blockage of an amilo ... | 1986 | 2867784 |
| activation of complement by pathogenic and nonpathogenic entamoeba histolytica. | previous studies had demonstrated that strains of entamoeba histolytica isolated from patients with colitis or amebic liver abscess were resistant to complement-mediated killing, whereas strains from asymptomatic patients were readily lysed by non-immune serum. both serum-sensitive and serum-resistant strains of e. histolytica depleted complement rapidly as assessed by ch50, c3, and c7, and c5-9 hemolytic activities. activation of the alternative pathway was important in lysis of nonpathogenic s ... | 1986 | 2869084 |
| patients treated for amebic liver abscess develop cell-mediated immune responses effective in vitro against entamoeba histolytica. | we studied the afferent and efferent cell-mediated immune response in 15 patients treated for amebic liver abscess. patients had a lower t4 to t8 ratio (1.25 +/- 0.65) compared with age- and sex-matched controls (1.89 +/- 0.44, p less than 0.01) due to a decrease in t4-"helper" cells and an increase in t8-"suppressor" cells (p less than 0.01). the in vitro proliferative response of patient t lymphocytes to the plant mitogen concanavalin a (con a) was depressed; responses to phytohemagglutinin we ... | 1986 | 2869086 |
| isoenzyme analysis of entamoeba histolytica isolated from homosexual men. | 1986 | 2869090 | |
| the major neutral proteinase of entamoeba histolytica. | fplc anion-exchange and chromatofocusing chromatography were used to purify the major neutral proteinase from secretions of axenically cultured entamoeba histolytica trophozoites. hm-1 strain trophozoites, which were more proteolytically active than the less virulent hk-9 strain, were used for purification of the enzyme. it is a thiol proteinase with a subunit mr of approximately 56,000, a neutral ph optimum, and a pi of 6. the importance of this enzyme in extraintestinal amoebiasis is suggested ... | 1986 | 2869098 |
| [intestinal parasite survey in seoul by stool examination at hanyang university hospital] | the present study was undertaken to evaluate the present status of intestinal parasitic infection in seoul area, korea. during the period from june 1985 to july 1986, a total of 5,251 stool samples were collected in department of clinical pathology, hanyang university hospital and examined by formalin-ether sedimentation technique once for helminth ova and protozoan cysts. the results were summerized as follows: the overall egg positive rate of intestinal helminthes was 2.53 per cent; and 1.43 p ... | 1986 | 12886099 |
| [a survey on intestinal parasites of soldiers in korea] | total of 2,643 korean soldiers were examined of their stool for parasitic infections by both cellophane thick smear and formalin-ether concentration techniques from august 1983 to december 1985. out of them, 73.6% were free from any parasite, 22.6% were ova positive and 4.0% cyst positive. the ova positive rates by species were ascaris lumbricoides 2.0%, trichuris trichiura 13.0%, hookworm and trichostrongylus orientalis 0.08% respectively, clonorchis sinensis 7.6%, metagonimus yokogawai 1.1%, p ... | 1986 | 12886100 |
| entamoeba histolytica spread by food. | 1986 | 18749951 | |
| clinical studies on amoebiasis and giardiasis evaluating the efficacy of kutaja (holarrhena antidysenterica) in entamoeba histolytica cyst passers. | the clinical study records the clinical presentations of forty cases with amoebias and / or giardiasis including the efficacy of kutaja (holarrhena antidysenterica) in intestinal amoebiasis. e. h. cyst passers also have symptoms like loose motions, constipation, flatulence, abdominal cramping, diminished appetite and mucus in stools. patients with giardiasis have more tendency to diarrhoea and flatulence with no mucus in stools. 70 per cent good response was observed in e. h. cyst passers when t ... | 1986 | 22557529 |
| [electron-microscopic studies on fine structure and enzyme activity in the axenic and conventional strains of entamoeba histolytica] | the metabolism of entamoeba histolytica would be affected by various environmental factors, and alteration of the environment was known to affect the fine structure of e. histolytica. the present study was designed electronmicroscopically to investigate the ultrastructure and enzyme activities in the axenic and conventional strains of e. histolytica. the trophozoites of axenically cultivated hk-9 strain and conventional ys-27 and ys-49 strains of e. histolytica were collected and fixed with 4 pe ... | 1985 | 12888671 |
| primates as a source of entamoeba histolytica, their zymodeme status and zoonotic potential. | a survey of 17 species of non-human captive primates in quebec, canada, revealed that 40% of the animals were infected with the protozoan entamoeba histolytica. isoenzyme analysis of lysates prepared from cultured amebic isolates showed them to belong to either a zymodeme iii or viii, and therefore, characteristic of non-pathogenic strains of e. histolytica from humans. these isolates were also of low virulence in gerbil ceca. serum samples from infected and uninfected primates had negative titr ... | 1985 | 2869116 |
| molecular mechanisms of cytotoxicity mediated by entamoeba histolytica: characterization of a pore-forming protein (pfp). | 1985 | 2868017 | |
| detection of amoebic antigen by enzyme linked immuno-sorbent assay (elisa). | 1985 | 2868123 | |
| growth studies on axenic entamoeba histolytica and naegleria fowleri. | 1985 | 2868550 | |
| antibodies to entamoeba histolytica in patients with rheumatoid arthritis. | 1985 | 2868552 | |
| acanthamoeba, an opportunistic microorganism: a review. | granulomatous amebic encephalitis due to acanthamoeba spp. usually occurs in chronically ill and debilitated individuals. some of these patients may have received immunosuppressive therapy. another infection due to acanthamoeba spp. has been corneal ulcerations which usually occur after minimal trauma to the corneal epithelium (1). in contrast, primary amebic meningoencephalitis due to naegleria fowleri usually occurs in healthy, young individuals with a history of swimming in heated swimming po ... | 1985 | 2867047 |
| [short-term treatment of protozoonoses with tiberal]. | 1985 | 2867648 | |
| lethal recognition between entamoeba histolytica and the host tissues. | lethal recognition between entamoeba histolytica and the tissues and defence systems of the host results in a continuous interplay that determines the development of pathological lesions: (i) we have identified several of the steps and mediators utilized by the trophozoites to destroy host cells by contact-mediated cytolysis; (ii) we have established that the alternative complement system represents the main defence available to the host against the invading parasite. the amoebae recognize targe ... | 1985 | 2420045 |
| entamoeba histolytica trophozoites in the lumen and mucus blanket of rat colons studied in vivo. | trophozoites of entamoeba histolytica hm-1 were cultivated axenically in tyi-s medium. the amoebae were then transferred into this medium lacking serum (tyi) and inoculated into in vivo colon loops of adult sprague-dawley rats. the trophozoites were rapidly absorbed by the mucus, and few were found free in the luminal fluid by 1 h. by 4 h, the amoebae began to reappear in the lumen, aggregated in sloughed mucus blanket fragments. the colon was examined histologically and by scanning electron mic ... | 1985 | 2856915 |
| seroepidemiological study of antibody responses to the zymodemes of entamoeba histolytica. | in 303 apparently healthy subjects and 54 patients with confirmed invasive amoebiasis amoebae were isolated where possible, and entamoeba histolytica was classified into its various zymodems. 20% of the healthy subjects were antibody-positive; 17 healthy subjects proved to be symptomless carriers of pathogenic zymodemes and, like patients with invasive disease, all these were seropositive. furthermore, of subjects infected with pathogenic zymodemes 94-100% were strongly seropositive, compared wi ... | 1985 | 2857995 |
| frequency distribution of entamoeba histolytica zymodemes in a rural south african population. | to determine the prevalence of entamoeba histolytica and its zymodeme distribution pattern, isoenzyme electrophoresis was performed on isolates of the parasite from 1381 individuals of the population of a semirural endemic area south of durban. about 10% of the population was found to harbour e histolytica. the zymodeme distribution was similar to that described in other surveys but two new zymodemes were observed, a non-pathogenic zymodeme xix and a pathogenic zymodeme xx. non-pathogenic zymode ... | 1985 | 2857996 |
| is that amoeba harmful or not? | 1985 | 2858001 | |
| [tropical mimics; study of the feces is a must]. | 1985 | 2858059 | |
| isolation, purification, and partial characterization of an enterotoxin from extracts of entamoeba histolytica trophozoites. | soluble cell-free extracts of pathogenic entamoeba histolytica, as well as serum-free minimal media in which trophozoites are incubated, contain substances that cause the rapid rounding up and detachment of tissue-cultured monolayers of mammalian cells (cytopathic activity) and induce fluid secretion in ligated intestinal loops of indomethacin-pretreated rats (enterotoxic activity). a semiquantitative assay for the determination of the cytopathic activity based on the rate of detachment of tissu ... | 1985 | 2858451 |
| entamoeba histolytica: early progressive pathology in the cecum of the gerbil (meriones unguiculatus). | the early formation of amebic cecal lesions in the gerbil (meriones unguiculatus) was studied sequentially from 1 to 10 days post-inoculation. the earliest response of the cecal mucosa towards trophozoites of entamoeba histolytica was local release of interglandular and goblet cell mucin. trophozoites adhered to the surface epithelium or were trapped in the luminal mucus in the absence of epithelial cell damage. focal mucosal erosion occurred later, and was preceded by crypt hyperplasia, edema a ... | 1985 | 2858986 |
| n-acetyl-d-galactosamine-inhibitable adherence lectin of entamoeba histolytica. i. partial purification and relation to amoebic virulence in vitro. | adherence of axenic entamoeba histolytica, strain hm1-imss, to chinese hamster ovary (cho) cells is mediated by an amoebic adhesin that is inhibited by n-acetyl-d-galactosamine (galnac). the in vitro virulence for cho cells and human neutrophils (pmns) of four strains of axenic amoebae was strain hm1-imss greater than h303-nih = 200-nih greater than nonvirulent laredo (p less than .001). the hm1 strain had the greatest sensitivity to galnac-mediated inhibition of adherence (p less than .001). ga ... | 1985 | 2859338 |
| n-acetyl-d-galactosamine-inhibitable adherence lectin of entamoeba histolytica. ii. mitogenic activity for human lymphocytes. | a soluble protein preparation from virulent axenic amoebae (strain hm1-imss) has been demonstrated to be mitogenic for normal human lymphocytes; mitogenesis was optimal at 100 micrograms/ml when incubation was maintained for five to seven days with monocytes (p less than .001). after gel filtration chromatography mitogenic activity of fractions was associated with n-acetyl-d-galactosamine-inhibitable lectin activity (agglutination of chinese hamster ovary cells; p less than .001). lymphocyte pro ... | 1985 | 2859339 |
| entamoeba histolytica and e. invadens: sulfhydryl-dependent proteolytic activity. | sodium dodecyl sulfate (sds) and 2-mercaptoethanol (2-me) activated proteolytic enzymes present in extracts of entamoeba histolytica and e. invadens; sds (0.5%) and 2-me (1.4 and 715 mm) doubled the enzymatic activity when assayed on a stained insoluble substrate. urea (4 m) did not reduce this activity, suggesting that amebic proteases are stable in the above denaturant conditions. specific reagents for sulfhydryl (-sh) groups completely inhibited proteolytic activity regardless of ph. inhibiti ... | 1985 | 2859367 |
| structural bases of the cytolytic mechanisms of entamoeba histolytica. | the cellular bases of the powerful cytolytic activity of the human protozoan parasite entamoeba histolytica were explored by studying the effect of the virulent strain hm1:imss on epithelial monolayers of mdck cells using a combination of time-lapse microcinematography and transmission and scanning electron microscopy. early alterations of the epithelial cell membranes were detected by measuring changes in the transepithelial electrical resistance of mdck monolayers mounted in ussing chambers. t ... | 1985 | 2859368 |
| amoebic isoenzymes. | 1985 | 2859504 |