Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [amebiasis: recent progress]. | 1987 | 2888181 | |
| a double-antibody sandwich elisa for the detection of entamoeba histolytica antigen in stool samples of humans. | a double-antibody sandwich elisa was developed to detect detergent-solubilized antigens of entamoeba histolytica in stool samples of humans. the test system was evaluated for its methodical and diagnostic sensitivity and specificity. in recovery experiments the lower limit of detection was 400 ng e. histolytica (hk9) protein/ml stool, corresponding to approximately 2000 amoebic trophozoites/ml stool. samples of 97 patients with suspected intestinal amoebiasis were examined. specific antigens wer ... | 1987 | 2888183 |
| [effect of the biotope and the intensity of invasion on the activity of various oxidation-reduction enzymes in entamoeba histolytica]. | 1987 | 2888240 | |
| [synthesis and antiparasitic activity of new nitro-5-imidazole derivatives]. | syntheses are given for derivatives of the type: 2-amino-1-[(1-methyl-5-nitro-2-imidazolyl) methyleneimino] imidazole, of new hydrazones of 1-methyl-5-nitroimidazole-2-carboxaldehyde and also for semicarbazones and a hydrazone of 1-methyl-5-nitro-2-(4-formyl-styryl)imidazole. | 1987 | 2888689 |
| recognition of the galactose- or n-acetylgalactosamine-binding lectin of entamoeba histolytica by human immune sera. | cure of amebic liver abscess is associated with resistance to recurrent invasive amebiasis and the development of a humoral and cell-mediated immune response. we determined whether human immune sera contain blocking antibody for the 170-kilodalton (kda) galactose or n-acetylgalactosamine (gal/galnac)-binding lectin of entamoeba histolytica. by western blot (immunoblot) of whole amebae subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all eight immune sera studied here promi ... | 1987 | 2888730 |
| complement activation in patients with amebic liver abscess. | serum or plasma concentrations of components of the classical (c1q, c4) and alternative (c3, factor b) pathways, regulatory protein factor h, and one of the c3 products of degradation, c3d, were determined in 19 patients with amebic liver abscess (ala). patients were divided into two groups. thirteen patients that recovered under medical treatment who had a significantly shorter clinical course on admission (p less than 0.05) (group 1) exhibited either normal (c1q; c4; factor b; c3d) or increase ... | 1987 | 2888804 |
| isolation of a 220-kilodalton protein with lectin properties from a virulent strain of entamoeba histolytica. | a 220-kilodalton (kda) protein with lectin properties was isolated from entamoeba histolytica strain hm1:imss and was purified by sepharose 4b chromatography and electroelution from 5% sds-polyacrylamide gels. the protein contains 9% carbohydrate by weight; is rich in hydrophobic residues; and is very immunogenic in mice, hamsters, and rabbits. the protein binds to fixed monolayers of mdck cells and inhibits trophozoite attachment to the cultured cells. the 220-kda protein agglutinates human ery ... | 1987 | 2888825 |
| use of antibodies to characterize a 220-kilodalton surface protein from entamoeba histolytica. | antibodies were prepared against a 220-kilodalton (kda) protein partially purified by sepharose 4b chromatography of entamoeba histolytica strain hm38:imss homogenates, and the protein was found to have lectin properties. the antibodies specifically recognized this protein in trophozoite homogenates. immunologically related molecules with the same molecular weight were identified by polyclonal antibodies in strains hm1:imss, entamoeba invadens, and entamoeba histolytica laredo. six monoclonal an ... | 1987 | 2888826 |
| unloaded polyisobutylcyanoacrylate nanoparticles: efficiency against bloodstream trypanosomes. | the potential use of polyisobutylcyanoacrylate nanoparticles in antiparasitic chemotherapy is described. the nanoparticles on their own proved to have trypanocidal activity against trypanosoma brucei brucei in-vitro but not against trichomonas vaginalis or entamoeba histolytica. the trypanocidal activity was partly confirmed in-vivo with t. brucei-infected mice. | 1987 | 2888859 |
| a fast electrophoretic isoenzyme technique for the identification of invasive and non-invasive entamoeba histolytica and "e. histolytica-like" organisms. | cellulose acetate electrophoresis (cae) was used to separate glucosephosphate isomerase, hexokinase, malic enzyme, and phosphoglucomutase extracted from invasive and non-invasive entamoeba histolytica and "e. histolytica-like" organisms. each of these morphologically similar organisms possessed a unique cae isoenzyme profile that can be used as an aid in their identification. the cae technique used to obtain these isoenzyme profiles is rapid, simple, and economical, and it requires neither speci ... | 1987 | 2888886 |
| in vitro evaluation of the activity of entamoeba histolytica studies on motility. | the tube migration test for leukocyte function studies has been modified to test the motility of entamoeba histolytica. the test was more sensitive to the effect of antiamebic drugs than was a test for phagocytic activity. a 50% reduction in amebic motility was seen instantly after exposure to metronidazole (40 mg/l). a similar reduction of phagocytosis at the same concentration of the drug was seen only after two hours' preexposure. the extent of amebic migration in the test tube is dependent o ... | 1987 | 2889170 |
| immunological studies in amebiasis: isotypic characterization of specific antibodies by enzyme-linked immunofiltration assay. | fifty sera from 17 cases of invasive amebiasis (15 hepatic localizations, 1 ameboma, 1 diffused colic localization) were studied by enzyme-linked immunofiltration assay (elifa). igm and ige anti-entamoeba histolytica were detected in 10 and 15, respectively, of the 17 patients studied. igm and ige antibodies were found simultaneously in 10 cases; these 2 isotypes were only recognized twice in the same serum by the same antigenic components. during post-therapy monitoring, in less than 90 days ig ... | 1987 | 2889384 |
| the electrophoretic isoenzyme patterns of strains of entamoeba histolytica isolated in two major cities in canada. | the isoenzyme patterns of 92 isolates of entamoeba histolytica from british columbia and 28 from ontario were determined. seropositivity for e. histolytica was assessed by indirect hemagglutination and enzyme-linked immunosorbent assay in the one center and by elisa and amebic gel diffusion in the other. in both british columbia and ontario nonpathogenic zymodemes i and iii were most common. a newly described isoenzyme pattern was identified in ontario. only 9 of 120 zymodeme patterns identified ... | 1987 | 2889385 |
| cholesterol induced changes in glucose-6-phosphate generating enzymes, concanavalin a agglutinability and haemolytic activity of axenic entamoeba histolytica. | repeated passage of the 200-nih strain of entamoeba histolytica through cholesterol-enriched axenic growth medium induced marked increases in cholesterol, phosphoglucomutase and hexokinase levels and a less prominent rise in the protein content of amoebic cells. there was also pronounced enhancement of haemolytic activity and concanavalin a (con a) agglutinability of the culture, but no significant change was observed in glucose phosphate isomerase. these cholesterol-induced effects persisted to ... | 1987 | 2889429 |
| a simplified in vitro assay correlating amebicidal activity with chelation by 8-hydroxyquinoline and related compounds. | 1987 | 2889432 | |
| interaction of pathogenic and nonpathogenic entamoeba histolytica with human complement. | 1987 | 2889433 | |
| isoenzyme patterns of strains of entamoeba histolytica isolated in british columbia. | 1987 | 2889434 | |
| the reliability of entamoeba histolytica zymodemes in clinical laboratory diagnosis. | 1987 | 2889435 | |
| specificity of a cysteine proteinase of entamoeba histolytica towards the alpha 1-cb2 peptide of bovine collagen type i. | the cysteine proteinase of entamoeba histolytica was shown to hydrolyze the cyanogen bromide peptide alpha 1-cb2 of calf skin collagen type i yielding two split products. amino acid analyses of the formed peptides and estimation of the amino-terminal residues revealed that the alpha 1-cb2 peptide was exclusively cleaved between gly10 and leu11 within the sequence -arg9-gly10-leu11-yielding two peptides with 7 and 29 amino acids, respectively. under identical conditions the ratio of hydrolysis of ... | 1987 | 2890103 |
| isolation and partial characterization of the hexokinase isoenzymes from pathogenic and non-pathogenic strains of entamoeba histolytica. | isoenzyme electrophoretic patterns (zymodemes) are increasingly used to distinguish between pathogenic and non-pathogenic strains of entamoeba histolytica. isolates of e. histolytica from asymptomatic and symptomatic cases have been shown to differ in the electrophoretic mobility of their hexokinase and phosphoglucomutase isoenzymes. the hexokinase isoenzymes from a non-pathogenic strain and from a pathogenic strain of e. histolytica were purified by fast protein liquid chromatography in several ... | 1987 | 2890104 |
| [confirmation of the identity of intestinal amoebae and evaluation of the virulence of entamoeba histolytica by isoenzyme electrophoresis]. | 1987 | 2890195 | |
| alterations of humoral, cell mediated and antibody dependent cell mediated cytotoxic responses during the course of amoebic infection in guinea pigs. | the cellular and antibody dependent cellular cytotoxic (adcc) responses of splenic lymphocytes and peritoneal macrophages obtained from animals at variable intervals after inoculation were studied against trophozoites of axenic e histolytica (nih:200). cytotoxic responses of effector cells from infected animals were compared with those of effector cells from vaccine stimulated and unstimulated uninfected control animals. cellular and antibody-dependent cellular cytotoxic responses of the effecto ... | 1987 | 2890558 |
| isolation of the galactose-binding lectin that mediates the in vitro adherence of entamoeba histolytica. | entamoeba histolytica adheres to human colonic mucus, colonic epithelial cells, and other target cells via a galactose (gal) or n-acetyl-d-galactosamine (galnac) inhibitable surface lectin. blockade of this adherence lectin with gal or galnac in vitro prevents amebic killing of target cells. we have identified and purified the adherence lectin by two methods: affinity columns derivatized with galactose monomers or galactose terminal glycoproteins, and affinity columns and immunoblots prepared wi ... | 1987 | 2890654 |
| rat and human colonic mucins bind to and inhibit adherence lectin of entamoeba histolytica. | establishment of adherence by entamoeba histolytica is mediated by a 170-kd gal/galnac inhibitable lectin and is required for cytolysis and phagocytosis of mammalian target cells. we studied the biochemical mechanisms of the in vitro interaction between rat and human colonic mucins and axenic e. histolytica trophozoites. crude mucus prevented amebic adherence to chinese hamster ovary (cho) cells by up to 70%. purification of the colonic mucins by sepharose 4b chromatography, nuclease digestion, ... | 1987 | 2890655 |
| entamoeba histolytica cyst passers--to treat or not to treat? | 1987 | 2891196 | |
| a longitudinal study of asymptomatic carriers of pathogenic zymodemes of entamoeba histolytica. | the value of treating subjects who pass entamoeba histolytica cysts in their faeces is currently in question. in the endemic durban area iso-enzyme electrophoresis of e. histolytica isolates indicated that 1% of the asymptomatic population are infected with pathogenic zymodemes. the outcome of these potentially invasive infections was established by means of a longitudinal survey. all subjects had strongly positive serological responses--10% of them developed amoebic colitis while the rest remai ... | 1987 | 2891197 |
| cellular and humoral mechanisms of cytotoxicity: structural and functional analogies. | 1987 | 2891261 | |
| longitudinal study on the health status of children in a rural tanzanian community: parasitoses and nutrition following control measures against intestinal parasites. | three repeated cross-sectional surveys were undertaken among children (1 month to 15 years) of a rural community in southeastern tanzania. the study was part of a longitudinal project on the interactions among nutrition, parasitic infections and immunity within a primary health care programme emphasizing village health workers. all children underwent interviews and parasitological, anthropometric, anamnestic and clinical examinations. out of 550-590 children examined each year, a cohort of 170 c ... | 1987 | 2891267 |
| immunoprotective behaviour of plasma-membrane-associated antigens of axenic entamoeba histolytica. | immunisation of golden hamsters with plasma-membrane-associated antigens of a virulent subline of axenic entamoeba histolytica strain nih 200 v, entrapped in multilamellar phosphatidylcholine liposomes (mpl) or freund's complete adjuvant (fca), afforded protection against intrahepatic challenge with axenic amoebic trophozoites of the same strain. amoebic liver abscess developed in 86% and 80% of the animals that received empty liposomes or buffer emulsified in fca but in none of the animals that ... | 1987 | 2891855 |
| iga protease in entamoeba histolytica trophozoites. | 1987 | 2892356 | |
| antiamoebic properties of human colostrum. | 1987 | 2892357 | |
| [entamoeba histolytica surface antigens that interact with specific human iga immune sera]. | 1987 | 2892474 | |
| entamoeba histolytica in homosexual men. | 1987 | 3821803 | |
| correlation of positive syphilis serology with invasive amebiasis in japan. | approximately 20% of 52 japanese males with invasive amebiasis confirmed by gel diffusion precipitin test (gdp) were positive by both treponema pallidum hemagglutination test (tpha) and nontreponemal antigen tests, and an additional 20% were positive by tpha alone. however, none of 109 gdp-negative japanese males without invasive amebiasis were positive on these serologic tests for syphilis. at least 2 bisexuals and 4 homosexuals were among those with invasive amebiasis. all of these biased male ... | 1987 | 3826490 |
| pathogenic and free-living protozoa cultured from the nasopharyngeal and oral regions of dental patients: ii. | protozoa of the nose, mouth, and pharynx of 30 randomly chosen male caries patients at an odontological clinic of the national autonomous university of mexico, in mexico city, were surveyed by culture from swabs. culture tubes from swabs were observed every other day for 5 weeks. pathogens found included entamoeba histolytica, naegleria fowleri, acanthamoeba castellanii, a. culbertsoni, a. polyphaga, and giardia lamblia. such isolations of pathogens suggest that patients may be healthy carriers ... | 1986 | 3956463 |
| entamoeba histolytica schaudinn, 1903: from xenic to axenic cultivation. | 1986 | 3959002 | |
| partial characterization of dna-dependent rna polymerases from entamoeba histolytica. | 1986 | 2884943 | |
| [analysis of the organization of the dna of various strains and clones of entamoeba histolytica]. | 1986 | 2884944 | |
| [development of a basic material for an axenic culture medium for entamoeba histolytica. ii. peptones]. | 1986 | 2884945 | |
| isolation and initial characterization of dna from entamoeba histolytica hm1:imss. | 1986 | 2884946 | |
| high salt sds-dep isolation of rna from entamoeba histolytica: the recovery of intact 25s rrna from amebae of different culture ages and the co-isolation of dna. | 1986 | 2884947 | |
| [castela texana: screening for its anti-amoebic activity]. | 1986 | 2884948 | |
| [localization and identification of adhemiba i, a protein which participates in the adhesion of entamoeba histolytica to human erythrocytes and epithelial cells]. | 1986 | 2884949 | |
| adhesion of entamoeba histolytica trophozoites to human erythrocytes. 1. maintenance of the integrity, viability and adhesion of trophozoites in different media. | 1986 | 2884950 | |
| adhesion of entamoeba histolytica trophozoites to human erythrocytes. 3. the inhibition patterns by carbohydrates are strain-specific. | 1986 | 2884951 | |
| [characterization of 3 mutants of entamoeba histolytica deficient in virulence with different phenotypes for adhesion and toxin activity]. | 1986 | 2884952 | |
| [mitogenic and lectin activity of mutants of entamoeba histolytica deficient in virulence]. | 1986 | 2884953 | |
| [multifactorial mechanism for the cytolytic activity of entamoeba histolytica]. | 1986 | 2884954 | |
| cytopathogenicity of entamoeba histolytica; a cytoplasmic heat stable factor stimulates dna synthesis in mammalian cells. | 1986 | 2884955 | |
| alteration of isoenzyme patterns of a cloned culture of non pathogenic entamoeba histolytica upon changes in growth conditions. | 1986 | 2884956 | |
| anti-surface antibodies to entamoeba histolytica and their role in complement lysis. | 1986 | 2884957 | |
| [acid phosphatase activity of 2 strains of entamoeba histolytica of different virulence]. | 1986 | 2884958 | |
| [diapedesis in experimental amebiasis]. | 1986 | 2884959 | |
| [experimental amebiasis in the guinea pig: a model for resistance]. | 1986 | 2884960 | |
| [immunogenicity of a delipidated antigen of entamoeba histolytica]. | 1986 | 2884961 | |
| [inhibition by antibodies of the adhesion of trophozoites of entamoeba histolytica]. | 1986 | 2884962 | |
| in vitro studies on the interaction of human monocytes and the monocyte locomotion inhibitory factor produced by e. histolytica. | 1986 | 2884963 | |
| [ultrastructural changes associated with the inhibition of human monocyte chemotaxis caused by products of e. histolytica cultured in axenic medium]. | 1986 | 2884964 | |
| [effect of supernatants obtained from lymphocytes stimulated with concanavalin a on trophozoites of entamoeba histolytica]. | 1986 | 2884965 | |
| [effect of cellular immunity on the interaction between the peritoneal cell and the ameba in vitro]. | 1986 | 2884966 | |
| [induction of a local humoral immune response against antigens of entamoeba histolytica]. | 1986 | 2884967 | |
| [detection of class iga antibodies directed against a lipopeptidophosphoglycan of e. histolytica in samples of human colostrum]. | 1986 | 2884968 | |
| [interaction in vitro of trophozoites of entamoeba histolytica with macrophages from human colostrum]. | 1986 | 2884969 | |
| [genetic transformation in entamoeba histolytica]. | 1986 | 2884970 | |
| [evaluation of cellular immunity in patients with amebic liver abscess using the leukocyte migration inhibition test]. | 1986 | 2884971 | |
| [clinical correlation of the zymodeme of entamoeba histolytica in patients in a psychiatric hospital]. | 1986 | 2884972 | |
| epidemiology of amebiasis in a rural community of mexico: serologic and coproparasitoscopic survey. | 1986 | 2884973 | |
| [cell-cycle phases of entamoeba histolytica]. | 1986 | 2884974 | |
| [epidemiology and control of amebiasis in mexico]. | 1986 | 2884975 | |
| amebiasis in the world. | 1986 | 2884976 | |
| [comparative study of zymodemes of entamoeba histolytica and the genus entamoeba in polyacrylamide gels]. | 1986 | 2884977 | |
| [production of e. histolytica hm-1 in agitated axenic culture and by fermenter]. | 1986 | 2884978 | |
| [purification of the polysaccharide portion of the lipopeptidophosphoglycan extracted from trophozoites of entamoeba histolytica]. | 1986 | 2884979 | |
| [characterization of the ribosomal proteins of entamoeba histolytica]. | 1986 | 2884980 | |
| [motility mutants of entamoeba histolytica resistant to cytochalasin d]. | 1986 | 2884981 | |
| [phases of nuclear division in entamoeba histolytica]. | 1986 | 2884982 | |
| in vitro studies on the sensitivity of local entamoeba histolytica to anti-amoebic drugs. | the in vitro activity of drugs, namely dehydroemetine, ornidazole, metronidazole and tinidazole were determined against the locally isolated strains of e. histolytica in thailand. the test was performed in liquid monophasic medium, i.e. liver marmite serum medium. in all, locally isolated strains from thirty hosts studied, the minimal inhibitory concentration (mic) for dehydroemetine ranged from 0.125 to 1 microgram/ml, ornidazole ranged from 0.0625 to 0.25 microgram/ml, metronidazole ranged fro ... | 1986 | 2883732 |
| studies of the antigenic activity of entamoeba histolytica extracts. | 1986 | 2884001 | |
| virulence of entamoeba histolytica correlates with the capacity to develop complement resistance. | 1986 | 2883155 | |
| lysozyme and complement-dependent lysis of entamoeba histolytica. inhibition by leupeptin. | 1986 | 2883156 | |
| human amoebiasis: cultivation and experimental transmission in animals. | in vitro cultivation was carried out on an initial strain of entamoeba histolytica from human source. dysphasic medium was used and concentrations of horse serum and antibiotic varied in response to developments. experimental infection of dogs, rabbits and mice was carried. except for the controls, all animals were orally infected and monitored until the experiment was terminated. parasitaemia became evident as from the 6th day in dogs experimentally infected and until about the 20th day when lo ... | 1986 | 2881905 |
| protection of mice against intestinal amoebiasis with bcg, corynebacterium parvum and listeria monocytogenes. | treatment with corynebacterium parvum or bcg, or infection with live listeria monocytogenes was found to protect mice against subsequent infection with entamoeba histolytica. complete protection was obtained in mice treated with 10(7) (colony forming units) of bcg but not with 10(5). partial protection was achieved with 10(6) of bcg. these data provide evidence that non-specific immunity plays an important role in the host defense against amoebic infection. | 1986 | 2880331 |
| in vivo effect of histamine & acetylcholine on the virulence of entamoeba histolytica. | 1986 | 2880802 | |
| [intestinal parasitic diseases of school children in the republic of niger]. | coprologic surveys realized in schoolchildren in a few western areas of the republic of niger provided the following results. concerning protozoa, the prevalence of amebiasis-infestation is strong in all the studies areas, giardiasis is more frequent in dry areas. as for the helminths, hymenolepsis nana is met everywhere, the prevalence of ancylostomiasis decreases gradually from the south to the north and the endemic area of schistosoma mansoni is limited to the extreme south of the country. th ... | 1986 | 2879647 |
| [in vitro effect of tinidazole on entamoeba histolytica]. | 1986 | 2879651 | |
| [molecular mechanisms in the pathogenicity of entamoeba histolytica]. | 1986 | 2879652 | |
| stimulation of dna synthesis on animal cell microcultures by a heat-stable cytoplasmic factor of entamoeba histolytica. | 1986 | 2870593 | |
| secondary cerebral amebiasis due to infection with entamoeba histolytica. a case report with computer tomographic findings. | the clinical and serological findings and computer tomographic (ct) appearances of a case of secondary cerebral amebiasis due to entamoeba histolytica are presented. the effect of therapy on the neurological symptoms as well as on the ct findings are described in detail. the multiple intracerebral lesions resolved almost completely and the patient survived without any neurological sequelae. | 1986 | 2870923 |
| distinction between entamoeba gingivalis and entamoeba histolytica, revisited. | 1986 | 2868598 | |
| cutaneous amebiasis of the face: a case report. | this paper describes a case of cutaneous amebiasis of the face in a 17-month-old girl. about four weeks prior to admission she had diarrhea with high suspicion of intestinal amebiasis. fifteen days later she presented with edema of the right lower eyelid and epiphora. there was a vesicle in the inner angle of the eye which spontaneously drained, causing loss of the underlying tissue. the ulcer developed rapidly, destroying periorbital tissue. both cytology and biopsy of the ulceration border sho ... | 1986 | 2868670 |
| is entamoeba histolytica in homosexual men a pathogen? | entamoeba histolytica (eh) in homosexual men is generally considered to be pathogenic. to test this hypothesis, the generally accepted features of invasion (haematophagous trophozoites in faeces; high-titre serum antibody; moderate to severe acute inflammatory change; and presence of eh in the mucosa on rectal biopsy) and the zymodeme pattern of cultured trophozoites were assessed in twenty-three eh excretors and eleven control homosexual men. no trophozoites or antibody to eh were found in eith ... | 1986 | 2869346 |
| fulminant amebic colitis in a homosexual man. | this report describes a case of fulminant amebic colitis leading to perforation and death in a 35-yr-old homosexual man. although entamoeba histolytica may be isolated from stool specimens in 20 to 30% of selected homosexual populations, reports of severe or invasive disease are rare. some workers have suggested that amebae are only passive colonizers of the colon in homosexual men. in our patient, pathological examination confirmed the presence of extensive colitis with penetration of amebae th ... | 1986 | 2869684 |
| cysteine proteinase of entamoeba histolytica. i. partial purification and action on different enzymes. | a method for a 50-60-fold purification of a cysteine proteinase from trophozoites of entamoeba histolytica using 35-80% ammonium sulphate fractionation, gel chromatography on sephadex g-75, and preparative isoelectric focusing is described. the enzyme was examined for its proteolytic potencies towards native enzyme substrates. the amebic proteinase directly inactivates aldolase and glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle as well as glucose-6-phosphate dehydrogenase from yeast ... | 1986 | 2870430 |
| cysteine proteinase of entamoeba histolytica. ii. identification of the major split position in bovine insulin b-chain. | the substrate specificity of a cysteine proteinase of entamoeba histolytica was tested using the oxidized b-chain of bovine insulin. 28 proteolytic peptides were formed after different intervals of incubation and could be separated by high-performance liquid chromatography. two dominant peptides appearing after 10 min of incubation were isolated and analyzed for amino acid composition. the results obtained indicate that these two peptides were formed by cleavage of the peptide bond between the a ... | 1986 | 2870431 |
| antibody-induced caps in entamoeba histolytica: isolation and electrophoretic analysis. | polyspecific antibodies bound to the cell surface of entamoeba histolytica are polarized toward the uroid region and spontaneously released by the amoeba as supramolecular aggregate or cap. we purified these exfoliated structures and analyzed them by electron microscopy and sodium dodecyl sulfate electrophoresis. isolated caps were mainly composed of membranes and contained five major [35s]methionine-labeled bands; many more bands appeared (together with immunoglobulins) after silver staining. s ... | 1986 | 2871113 |
| amebiasis: introduction, current status, and research questions. | this series of five papers on the worldwide problem of amebiasis, its current status, research needs, and opportunities for progress has grown out of a renewal of investigative interest in amebiasis. the recent development of in vitro culture methods and of means to distinguish strain differences and the application of modern tools of cellular biology and biochemistry have raised new questions regarding strain definitions, virulence traits, host defenses, and the mechanism of invasive disease so ... | 1986 | 2871618 |
| problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. | major problems with a wide array of imperfect tests for diagnosis of amebiasis severely limit the understanding of its magnitude and epidemiology. a greater hindrance is the varied, inconsistent application of existing methods in different areas of the world. the best estimates suggest that probably 480 million people were infected with entamoeba histolytica and 36 million developed disabling colitis or extraintestinal abscesses in 1981. at least 40 thousand deaths are attributable to amebiasis, ... | 1986 | 2871619 |
| immunologic tools in the diagnosis of amebiasis: epidemiology in the united states. | several immunodiagnostic tests that detect antibody to entamoeba histolytica are now available. such tests are useful when demonstration of the etiologic agent is difficult (as in liver abscess), when routine diagnostic tests fail to disclose the organisms (as in severe intestinal disease compromised by failure of the laboratory to identify the organism or by the patient's use of interfering substances), and as epidemiologic tools for determining the prevalence of invasive disease in populations ... | 1986 | 2871620 |
| pathogenesis of disease caused by entamoeba histolytica: studies of adherence, secreted toxins, and contact-dependent cytolysis. | amebiasis is due to invasion of the colonic mucosa by the enteric protozoan entamoeba histolytica. the biology of the parasite and the pathogenic mechanisms of e. histolytica have been extensively studied. recent studies provide new insights into the mechanism by which amebas colonize the gut, interact with bacterial flora, adhere to and lyse tissue, and combat the host's inflammatory response. elucidation of the biochemical and cellular bases of the pathogenesis of disease caused by e. histolyt ... | 1986 | 2871621 |
| review of the human immune mechanisms directed against entamoeba histolytica. | the human immune mechanisms effective against the destructive parasite entamoeba histolytica have not been established. humoral responses develop with invasive disease but have not positively correlated with protection against recurrent invasive infection. though complement is amebicidal, invasive strains of amebas may be resistant. polymorphonuclear neutrophils have not been active in vitro against virulent amebic strains and could contribute to the pathogenesis of disease. in vitro studies hav ... | 1986 | 2871622 |