Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| comparative glycomics of fat globule membrane glycoconjugates from buffalo (bubalus bubalis) milk and colostrum. | the health-promoting effects of milk fat globule membrane (mfgm) glycoconjugates has attracted curiosity especially with regard to the challenges encountered to unravel the glycan complexities of mfgm glycoproteins and glycosphingolipids. in this context, we characterized glycans present in buffalo milk and colostrum fat globule membranes by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms analysis by adopting chemoselective glycoblotting technique. unlike human and bovi ... | 2017 | 28145111 |
| a brief and critical review on hydrofluorosis in diverse species of domestic animals in india. | india is one of the fluoride-endemic countries where the maximum numbers of ground or drinking water sources are naturally fluoridated. in india, a total of 23, out of 36 states and union territories have drinking water contaminated with fluoride in varying concentration. in the present scenario, especially in rural india, besides the surface waters (perennial ponds, dams, rivers, etc.), bore wells and hand pumps are the principal drinking water sources for domestic animals such as cattle (bos t ... | 2017 | 28144801 |
| identification of genetic variation in nod-like receptor 2 gene and influence of polymorphism on gene structure and function in buffalo (bubalus bubalis). | nucleotide-binding oligomerization domain (nod)-like receptor 2 is one of the important mediators of innate as well as adaptive immune response to microbial infections. in this study, nod-like receptor-2 was characterized by determining the full gene sequence and analyzing genetic diversity in indian buffaloes. sequence analysis of buffalo nod2 revealed 3042 nucleotides long orf, encoding 1013 amino acids from 12 exons. domain structure analysis indicated existence of 8 leucine-rich repeat (lrr) ... | 2017 | 28135669 |
| molecular characterization of hoxc8 gene and methylation status analysis of its exon 1 associated with the length of cashmere fiber in liaoning cashmere goat. | homeobox protein hox-c8 (hoxc8) is a member of hox family. it is expressed in the dermal papilla of the skin and is thought to be associated with the hair inductive capacity of dermal papilla cells. in the present study, we isolated and characterized a full-length open reading frame of hoxc8 cdna from the skin tissue of liaoning cashmere goat, as well as, established a phylogenetic relationship of goat hoxc8 with that of other species. also, we investigated the effect of methylation status of ho ... | 2017 | 28093668 |
| regucalcin is widely distributed in the male reproductive tract and exerts a suppressive effect on in vitro sperm capacitation in the water buffalo (bubalus bubalis). | regucalcin is a multi-functional, calcium-binding protein with roles in calcium homeostasis, apoptosis, cell proliferation, and free radical neutralization. regucalcin is broadly expressed in the male reproductive organs of rat and bovine; here, we report its expression in the reproductive tract of male buffalo-especially in testis, epididymis, seminal vesicle, prostate, and bulbourethral gland of buffalo-as analyzed by real-time pcr, western blot, and immunolocalization. regucalcin degradation ... | 2017 | 27987309 |
| validation of a fast real-time pcr method to detect fraud and mislabeling in milk and dairy products. | fast real-time pcr taqman assays were developed and validated for species identification in dairy products. based on the amplification of 12s rrna and cytb partial genes of mitochondrial dna, the methods were demonstrated to be sensitive, fast, and species-specific for bos taurus, ovis aries, bubalus bubalis, and capra hircus. the limit of detection calculated was lower than 1%, and the efficiency was reported to be higher than 96% in every assay. an internal amplification control was used to de ... | 2017 | 27865504 |
| alginate encapsulation preserves the quality and fertilizing ability of mediterranean italian water buffalo (bubalus bubalis) and holstein friesian (bos taurus) spermatozoa after cryopreservation. | the use of artificial insemination (ai) in buffalo (bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells in the female tract, difficulties in estrus detection, and variable estrus duration. although ai procedures are commonly used in bovine, use of ai has been limited in buffalo. in the zootechnical field, different studies have been conducted to develop techniques for improvement of fertilizing ability ... | 2017 | 27456772 |
| hsfy and znf280by show copy number variations within 17 water buffalo populations. | recent transcriptomic analysis of the bovine y chromosome revealed abundant presence of multi-copy protein coding gene families on the male-specific region of the y chromosome (msy). copy number variations (cnvs) of several msy genes are closely related to semen quality and male reproduction in cattle. however, the cnvs of msy genes in water buffalo are largely unknown. therefore, this study aimed to investigate the cnvs of hsfy and znf280by of 298 buffaloes from 17 populations distributed in ch ... | 2017 | 27739082 |
| heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview. | hemostatic and adhesive agents date back to world war ii, when homologous fibrin sealant came onto scene. considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. its components were a serine protease (a thrombin-like enzyme) extracted from the venom of crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of bubalus bubalis buffaloes. this new bioproduct has been used as ... | 2017 | 28396682 |
| comparative antinociceptive and sedative effects of epidural romifidine and detomidine in buffalo (bubalus bubalis). | in this study, comparative antinociceptive and sedative effects of epidural administration of romifidine and detomidine in buffalo were evaluated. eighteen healthy adult buffalo, allocated randomly in three groups (two experimental and one control; n=6) received either 50 μg/kg of romifidine or detomidine diluted in sterile saline (0.9 per cent) to a final volume of 20 ml, or an equivalent volume of sterile saline epidurally. antinociception, sedation and ataxia parameters were recorded immediat ... | 2017 | 28386033 |
| profile and artificial insemination practices of technicians and the artificial insemination success rates in leyte, samar, and biliran, philippines (2011-2015). | artificial insemination (ai) is a reproductive biotechnology that may be influenced by several factors, including the profile of the technicians and the practices used. assessing technician's profile and their ai practices can be significant in improving ai success rate. | 2017 | 28344401 |
| relationship between mozzarella yield and milk composition, processing factors, and recovery of whey constituents. | our aim was to identify the relationship between mozzarella cheese yield and buffalo milk composition, processing factors, and recovery of whey constituents. a production of 30 batches of mozzarella cheese at a dairy industry in northeast brazil (rio grande do norte) was monitored between march and november, 2015. mozzarella yield and 32 other variables were observed for each batch, and divided into 3 groups: milk composition variables (12); variables involved in the cheesemaking process (14); a ... | 2017 | 28342605 |
| empirical and bioinformatic characterization of buffalo (bubalus bubalis) colostrum whey peptides & their angiotensin i-converting enzyme inhibition. | whey based peptides are well known for their nutritional and multifunctional properties. in this context, whey proteins from buffalo colostrum & milk were digested by in vitro simulation digestion and analyzed by nano-lc-ms/ms. functional protein association networks, gene annotations and localization of identified proteins were carried out. an ace inhibitory peptide sorted from the library was custom synthesized and an in vitro ace assay was performed. the study led to the identification of 74 ... | 2017 | 28317767 |
| changes in the total lipid, neutral lipid, phospholipid and fatty acid composition of phospholipid fractions during pastırma processing, a dry-cured meat product. | pastırma is a dry-cured meat product, produced from whole beef or water buffalo muscles. this study was carried out to investigate the effect of production stages (raw meat, after curing, after 2(nd) drying and pastırma) on the total lipid, neutral lipid, phospholipid and fatty acid composition of phospholipid fraction of pastırma produced from beef m. longissimus dorsi muscles. the ph and colour (l*, a* and b*) analyses were also performed in raw meat and pastırma. it was found that pastırma pr ... | 2017 | 28316467 |
| differential surface glycoprofile of buffalo bull spermatozoa during mating and non-mating periods. | the buffalo has a seasonal reproduction activity with mating and non-mating periods occurring from late autumn to winter and from late spring to beginning of autumn, respectively. sperm glycocalyx plays an important role in reproduction as it is the first interface between sperm and environment. semen quality is poorer during non-mating periods, so we aimed to evaluate if there were also seasonal differences in the surface glycosylation pattern of mating period spermatozoa (mps) compared with no ... | 2017 | 28264742 |
| spermatozoa with high mitochondrial membrane potential and low tyrosine phosphorylation preferentially bind to oviduct explants in the water buffalo (bubalus bubalis). | although it is understood that spermatozoa are subjected to selection processes to form a functional sperm reservoir in the oviduct, the mechanism remains obscure. with the aim to understand the sperm selection process in the oviduct, in the present in vitro study, we analyzed mitochondrial membrane potential and tyrosine phosphorylation status in oviduct-explants bound and unbound spermatozoa. frozen semen from murrah buffalo bulls (n=10) used under progeny testing programme were utilized for t ... | 2017 | 28262463 |
| functional gene profiling through metarnaseq approach reveals diet-dependent variation in rumen microbiota of buffalo (bubalus bubalis). | recent advances in next generation sequencing technology have enabled analysis of complex microbial community from genome to transcriptome level. in the present study, metatranscriptomic approach was applied to elucidate functionally active bacteria and their biological processes in rumen of buffalo (bubalus bubalis) adapted to different dietary treatments. buffaloes were adapted to a diet containing 50:50, 75:25 and 100:0 forage to concentrate ratio, each for 6 weeks, before ruminal content sam ... | 2017 | 28246035 |
| identification and evaluation of reference genes for accurate gene expression normalization of fresh and frozen-thawed spermatozoa of water buffalo (bubalus bubalis). | the quantitative real time pcr (qrt-pcr) has become an important tool for gene-expression analysis for a selected number of genes in life science. although large dynamic range, sensitivity and reproducibility of qrt-pcr is good, the reliability majorly depend on the selection of proper reference genes (rgs) employed for normalization. although, rgs expression has been reported to vary considerably within same cell type with different experimental treatments. no systematic study has been conducte ... | 2017 | 28237344 |
| functional involvement of protein kinase c, rho-kinase and trpc3 decreases while plc increases with advancement of pregnancy in mediating oxytocin-induced myometrial contractions in water buffaloes (bubalus bubalis). | present study unravels the involvement of different calcium signaling pathways in oxytocin-induced contractions in myometrium of non-pregnant and pregnant buffaloes during early and mid-pregnancy stages. uteri of pregnant animals were more sensitive than of non-pregnant buffaloes. phasic contractions and frequency of contraction significantly increased with advancement of pregnancy, while tonic contractions non-significantly and amplitude significantly decreased from six months pregnancy onward. ... | 2017 | 28237334 |
| effect on quanti-quality milk and mozzarella cheese characteristics with further increasing the level of dried stoned olive pomace in diet for lactating buffalo. | following a previous report, an experiment was conducted to determine the effect of increasing level of dsop (dried stoned olives pomaces) in the diet of lactating buffaloes on milk and mozzarella cheese yield and characteristics. | 2017 | 28231700 |
| effect of feeding of blend of essential oils on methane production, growth, nutrient utilization in growing buffaloes. | an experiment was conducted to study the effect of a blend of essential oils (beo) on enteric methane emission and growth performance of buffaloes (bubalus bubalis). | 2017 | 28231698 |
| extra and intracellular calcium signaling pathway(s) differentially regulate histamine-induced myometrial contractions during early and mid-pregnancy stages in buffaloes (bubalus bubalis). | this study examines the differential role of calcium signaling pathway(s) in histamine-induced uterotonic action during early and mid-pregnancy stages in buffaloes. compared to mid pregnancy, tonic contraction, amplitude and mean-integral tension were significantly increased by histamine to produce myometrial contraction during early pregnancy with small effects on phasic contraction and frequency. although uterotonic action of histamine during both stages of pregnancy is sensitive to nifedipine ... | 2017 | 28228338 |
| cryoprotectant effect of trehalose in extender on post-thaw quality and in vivo fertility of water buffalo (bubalus bubalis) bull spermatozoa. | this study was designed to ascertain the cryoprotectant effects of different concentrations of trehalose [0 (t0), 25 (t25), 35 (t35), 45 (t45) mm], egg yolk [20% (e20), 15% (e15) v/v] and glycerol [7% (g7), 5% (g5) v/v] in tris-citric acid-based extender on post-thaw quality and in vivo fertility of buffalo bull spermatozoa. twenty-five ejaculates were collected from five bulls and split into four parts. after that, the split ejaculates from each of the bull were diluted either in t0e20g7 (contr ... | 2017 | 28224657 |
| basic fibroblast growth factor is critical to reprogramming buffalo (bubalus bubalis) primordial germ cells into embryonic germ stem cell-like cells. | primordial germ cells (pgcs) are destined to form gametes in vivo, and they can be reprogrammed into pluripotent embryonic germ (eg) cells in vitro. buffalo pgc have been reported to be reprogrammed into eg-like cells, but the identities of the major signaling pathways and culture media involved in this derivation remain unclear. here, the effects of basic fibroblast growth factor (bfgf) and downstream signaling pathways on the reprogramming of buffalo pgcs into eg-like cells were investigated. ... | 2017 | 28215675 |
| congenital malformations in river buffalo (bubalus bubalis). | the world buffalo population is about 168 million, and it is still growing, in india, china, brazil, and italy. in these countries, buffalo genetic breeding programs have been performed for many decades. the occurrence of congenital malformations has caused a slowing of the genetic progress and economic loss for the breeders, due to the death of animals, or damage to their reproductive ability or failing of milk production. moreover, they cause animal welfare reduction because they can imply foe ... | 2017 | 28208595 |
| urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed-artificial insemination in murrah buffalo (bubalus bubalis). | assessment of urine levels of luteinizing hormone (lh) for predicting the reproductive status of animals is in practice. the aim of this study was to predict the period of ovulation based on the urine levels of lh for timed-artificial insemination to increase the conception rate in buffaloes, which are naturally silent-oestrous animals. level of lh in urine was assessed using elisa, and a cut-off lh concentration for prediction of ovulation period was obtained using receiver operating characteri ... | 2017 | 28176388 |
| study of gastrointestinal parasites in water buffalo (bubalus bubalis) reared under mexican humid tropical conditions. | the objective was to determine the frequency of gastrointestinal parasites (gp) genera affecting water buffalo (bubalus bubalis) reared under humid tropical conditions of mexico. three hundred eighty-three munrah breed water buffalo were included, 251 adult females and 132 calves. feces were directly collected form the rectum of the animals and processed by the mcmaster technique. coprocultures were made to identify the genera of the nematodes. the frequency of gp in b. bubalis was 42%, independ ... | 2017 | 28161847 |
| inhibition of snake venom metalloproteinase by β-lactoglobulin peptide from buffalo (bubalus bubalis) colostrum. | bioactive peptide research has experienced considerable therapeutic interest owing to varied physiological functions, efficacy in excretion, and tolerability of peptides. colostrum is a rich natural source of bioactive peptides with many properties elucidated such as anti-thrombotic, anti-hypertensive, opioid, immunomodulatory, etc. in this study, a variant peptide derived from β-lactoglobulin from buffalo colostrum was evaluated for the anti-ophidian property by targeting snake venom metallopro ... | 2017 | 28155167 |
| identification and analysis of the expression of microrna from lactating and nonlactating mammary glands of the chinese swamp buffalo. | to study the role of microrna (mir) in the lactation physiology of water buffalo, 2 multiparous dairy buffaloes (including an 8-yr-old buffalo that had been lactating for 3 mo, as well as a 10-yr-old nonlactating, nonpregnant buffalo) were used for mir library construction. the profile of differentially expressed mir in lactating and nonlactating mammary gland tissues of these water buffalo were investigated using illumina-solexa high-throughput sequencing technology (illumina, san diego, ca). t ... | 2017 | 28109598 |
| direct and indirect effects of wastewater use and herd environment on the occurrence of animal diseases and animal health in pakistan. | the use of wastewater for rearing domestic animals is a common phenomenon in most of the developing countries like pakistan that face a serious shortage of freshwater resources. however, most of the literature has only focused on the indirect effects of wastewater use on animal health or productivity, and literature on the direct effects of wastewater use is rare. therefore, this study aims to investigate the direct and indirect effects of wastewater usage on the prevalence of animal diseases an ... | 2017 | 28092000 |
| causes and mechanisms of death in fatal water buffalo attacks. | deaths due to buffalo attack have not been well described. a 72-year-old man was trampled by a water buffalo (bubalus bubalis) while attempting to move the animal within an enclosed area at an abattoir. at autopsy, there were numerous injuries involving the chest, head, neck, and left upper arm. blunt force injury had resulted in multiple rib fractures with a flail chest and fracture/dislocation of the mid-cervical vertebrae caused either by physical crushing by the animal against the walls of t ... | 2017 | 28066906 |
| valproic acid increases histone acetylation and alters gene expression in the donor cells but does not improve the in vitro developmental competence of buffalo (bubalus bubalis) embryos produced by hand-made cloning. | use of histone deacetylase inhibitors (hdacis) is believed to improve the developmental competence and quality of cloned embryos produced. we examined the effects of treatment of buffalo fibroblasts with valproic acid (vpa), a hdaci on these cells and on embryos produced from them by hand-made cloning. vpa treatment (1.5, 3.0, or 4.5 mm) altered (p < 0.05) the growth characteristics and relative expression level of hdac1, dnmt1, dnmt3a, p53, and caspase3, and the global level of h3k9/14ac, h4k5a ... | 2017 | 28055238 |
| chicken egg yolk plasma in tris-citric acid extender improves the quality and fertility of cryopreserved water buffalo (bubalus bubalis) spermatozoa. | this study was primarily designed to evaluate the effect of different concentrations of ultraviolet (uv)-c-irradiated chicken egg yolk plasma (eyp; v:v; 10%, p1; 15%, p2; 20%, p3) or 20% (v:v) of whole chicken egg yolk (wcey) in tris-citric acid (tca) extender on water buffalo sperm quality during cryopreservation (postdilution, pd; postequilibration, pe; post-thawing, pt). also the effect of best evolved concentration of uv-c-irradiated eyp in extender on in vivo fertility of buffalo spermatozo ... | 2017 | 28043368 |
| using real-time pcr to identify pregnancy-associated glycoprotein 2 (pag-2) in water buffalo (bubalus bubalis) blood in early pregnancy. | this study investigates for the first time mrna pregnancy-associated glycoprotein 2 (pag-2) expression in blood cells during early pregnancy in water buffalo. the pags constitute a large family of glycoproteins expressed in the outer epithelial layer of the placenta in eutherian species. all pags are not concomitantly expressed throughout pregnancy; some of them are expressed in the earlier phases, whereas others appear later and are expressed over a shorter period. twenty-one lactating buffaloe ... | 2017 | 28043340 |
| impact of heat stress on cellular and transcriptional adaptation of mammary epithelial cells in riverine buffalo (bubalus bubalis). | the present study aims to identify the heat responsive genes and biological pathways in heat stressed buffalo mammary epithelial cells (mecs). the primary mammary epithelial cells of riverine buffalo were exposed to thermal stress at 42°c for one hour. the cells were subsequently allowed to recover at 37°c and harvested at different time intervals (30 min to 48 h) along with control samples (un-stressed). in order to assess the impact of heat stress in buffalo mecs, several in-vitro cellular par ... | 2017 | 27682256 |
| estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: application for fertility assessment in buffalo (bubalus bubalis) bulls. | this study was attempted to identify subfertile bulls by quantifying the endogenous levels of osteopontin (opn), total antioxidant capacity (tac) and malondialdehyde (mda) in seminal plasma of buffalo bulls. on the basis of conception rate, buffalo bulls were classified into two groups: high-fertile (conception rate >50%) and subfertile bulls (conception rate <40%). a total of 100 ejaculates (10 ejaculates from each bull) were collected through artificial vagina method. the concentration of opn, ... | 2017 | 27862407 |
| peptidome characterization of the antipyretic fraction of bubali cornu aqueous extract by nano liquid chromatography with orbitrap mass spectrometry detection. | in the present study, the antipyretic activity of bubali cornu (water buffalo horn) fraction and its metabolomics were investigated. the fraction decreased rat rectal temperature, and 13 endogenous metabolites were identified as potential biomarkers. selected metabolites were involved in arachidonic acid metabolism and glycerophospholipid metabolism etc. following treatment with the fraction, four metabolites, pyroglutamic acid, palmitelaidic acid, leukotriene a4, and prostaglandin a2 were rever ... | 2017 | 27860201 |
| expression and functional role of bone morphogenetic proteins (bmps) in cyclical corpus luteum in buffalo (bubalus bubalis). | the role of growth factors in the modulation of ovarian function is an interesting area of research in reproductive biology. recently, we have shown the expression and role of igf, egf, vegf and fgf in the follicle and cl. here, we report the presence of bone morphogenetic proteins (bmps) and their functional receptors in the corpus luteum (cl) of buffalo. the bubaline cl was classified into four stages according to the morphology and progesterone (p4) concentration. the qpcr, immunoblot and imm ... | 2017 | 27815159 |
| cholesterol-loaded cyclodextrins prevent cryocapacitation damages in buffalo (bubalus bubalis) cryopreserved sperm. | the aim of this study was to investigate the effect of cholesterol-loaded cyclodextrins (clc) on motility, viability, capacitation status, and in vivo fertility of buffalo frozen-thawed sperm. after the initial semen assessment, buffalo sperm were diluted in bullxcell extender containing 0- (control), 1.5-, and 3-mg/ml clc and cryopreserved. at thawing, sperm motility was evaluated by phase contrast microscopy, and viability-capacitation status was assessed by hoechst 33258-chlortetracycline (ct ... | 2017 | 27793453 |
| the nitric oxide serum level and combined utero-placental thickness in buffalo (bubalus bubalis) affected by pregnancy pathology. | the present study aimed mainly to study the level of nitric oxide (no) and combined thickness of the uterus and placenta (ctup) in buffaloes affected by pregnancy pathology. females (n = 104) were classified into three main groups: nonpregnant (n = 10), healthy pregnant (n = 54), and pathologically pregnant (n = 40). the healthy pregnant animals were subgrouped according to the stage of pregnancy to early stage (n = 15), mid stage (n = 15), late stage (n = 19), and full term (n = 5). the animals ... | 2017 | 27693011 |
| the tumor necrosis factor g1022g>a polymorphism is associated with resistance to tuberculosis in water buffalo (bubalus bubalis). | 2017 | 27739092 | |
| use of post-thaw semen quality parameters to predict fertility of water buffalo (bubalus bubalis) bull during peak breeding season. | this study was designed to predict the fertility of water buffalo bull using post-thaw semen quality parameters during peak breeding season. thirty ejaculates were collected from five bulls with artificial vagina and cryopreserved. at post-thaw, semen was analysed for motility parameters, velocity distribution, kinematics, dna integrity/fragmentation, viability, mitochondrial transmembrane potential, morphology, plasma membrane and acrosome integrity. data of 514 inseminations were collected for ... | 2017 | 27401143 |
| effect of egg yolk powder on freezability of murrah buffalo (bubalus bubalis) semen. | the aim of this study was to investigate the effect of commercial egg yolk powder as an alternative to fresh egg yolk on freezability of murrah buffalo semen. | 2016 | 27397983 |
| modulatory role of leptin on ovarian functions in water buffalo (bubalus bubalis). | the aim of the present study was to demonstrate the modulatory role of leptin on bubaline granulosa cells (gcs) and luteal cells (lcs) functions using an in vitro cell culture system and to establish a cross talk between leptin and insulin-like growth factor-1 (igf-1). gcs were collected from group iv follicles (>13 mm size) and lcs from mid-luteal phase corpus luteum and were grown in serum-containing media supplemented with leptin at three different dose rates (0.1, 1, and 10 ng/ml) and time d ... | 2016 | 27381558 |
| efficiency of beetle (dendroides canadensis) recombinant antifreeze protein for buffalo semen freezability and fertility. | overwintering larvae of the beetle dendroides canadensis produce potent antifreeze proteins to inhibit inoculative freezing and promote supercooling. we hypothesized that addition of dendroides canadensis recombinant antifreeze proteins (dafps) in the extender will improve the quality and fertility of cryopreserved nili-ravi buffalo (bubalus bubalis) sperm. the study was divided into two parts: (1) evaluation of the effect of dafps on the quality of frozen-thawed buffalo bull sperm and (2) exami ... | 2016 | 27349137 |
| serological investigation of leptospira infection and its circulation in one intensive-type water buffalo farm in the philippines. | water buffalo is an indispensable livestock in the philippines. leptospirosis is a serious zoonosis that can be fatal to humans and cause reproductive problems in livestock. leptospirosis has been reported in some countries where water buffaloes are commercially raised, highlighting the leptospira prevalence in this farming system, but information on leptospirosis in water buffalo farms in the philippines is limited. in this study, we collected blood samples from rats (n = 21), and water buffalo ... | 2016 | 27348885 |
| correction: comparative pharmacokinetics of ceftiofur hydrochloride and ceftiofur sodium after administration to water buffalo (bubalus bubalis). | 2016 | 27347830 | |
| semen quality parameters as fertility predictors of water buffalo bull spermatozoa during low-breeding season. | the present study was carried out to assess various postthaw semen quality parameters for the prediction of fertility in buffalo bull during low-breeding season. semen (30 ejaculates) was collected from five adult buffalo bulls with artificial vagina (42 °c). sperm motility parameters, velocity distribution, motion kinematics, and subpopulations were analyzed by computer-aided sperm motion analyzer (casa). moreover, sperm visual motility, supravital plasma membrane integrity, viability/acrosome ... | 2016 | 27321805 |
| clinical and ultrasonographic observations of functional and mechanical intestinal obstruction in buffaloes (bubalus bubalis). | this study was designed for clinical and laboratory evaluation of intestinal obstruction (io) in buffaloes (bubalus bubalis) with special emphasis on the diagnostic value of ultrasonographic findings. | 2016 | 27284223 |
| seasonal and ageing-depending changes of aquaporins 1 and 9 expression in the genital tract of buffalo bulls (bubalus bubalis). | the presence of aquaporins 1 (aqp1) and 9 (aqp9), integral membrane water channels that facilitate rapid passive movement of water and solutes, was immunohistochemically detected in the excurrent ducts collected from sexually mature buffalo bulls of proven fertility during the mating (late autumn-winter) and non-mating (late spring to the beginning of autumn) seasons. furthermore, the research was performed also on the epididymal cauda of a senile buffalo bull with inactive testis. aquaporins 1 ... | 2016 | 27260501 |
| igf1 stabilizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (bubalus bubalis) spermatozoa. | insulin like growth factor 1 (igf1) in the seminal plasma is reported to improve sperm motility by reducing oxidative stress. the present study was conducted to assess the effect of addition of igf1 on sperm function and protein composition during cryopreservation process. semen samples were collected from six murrah buffaloes (2 ejaculates from each animal) and diluted (80 million/ml) in tris egg yolk extender and divided into control, t1, t2 and t3, groups supplemented with 0, 50, 100 and 150 ... | 2016 | 27256665 |
| use of peripheral blood for production of buffalo (bubalus bubalis) embryos by handmade cloning. | buffalo embryos were produced by handmade cloning using peripheral blood-derived lymphocytes as donor cells. although the blastocyst rate was lower (p < 0.01) for lymphocyte- than control skin fibroblast-derived embryos (6.6 ± 0.84% vs. 31.15 ± 2.97%), the total cell number (152.6 ± 23.06 vs. 160.1 ± 13.25) and apoptotic index (6.54 ± 0.95 vs. 8.45 ± 1.32) were similar. the global level of h3k9ac was higher (p < 0.05) in lymphocyte- than that in skin-derived blastocysts; whereas in ivf blastocys ... | 2016 | 27242179 |
| genetic and genomic dissection of prolactin revealed potential association with milk production traits in riverine buffalo. | milk yield and quality has been a major selection criterion for genetic improvement in livestock species. role of prolactin gene in determining milk quality in terms of protein profile, lactose, lipids and other imperative macromolecules is very important. in this context, genetic profiling of prolactin gene in riverine buffalo of pakistan was performed and potential genetic markers were identified illustrating worth of this gene in marker-assisted selection of superior dairy buffaloes. series o ... | 2016 | 27240674 |
| comparative pharmacokinetics of ceftiofur hydrochloride and ceftiofur sodium after administration to water buffalo (bubalus bubalis). | objective to evaluate pharmacokinetics and bioavailability after administration of ceftiofur hydrochloride and ceftiofur sodium to water buffalo (bubalus bubalis). animals 5 healthy adult water buffalo (3 males and 2 nonlactating females). procedures all animals received a dose (2.2 mg/kg) of 3 ceftiofur products (2 commercially available suspensions of ceftiofur hydrochloride [cef1 and cef2, im] and ceftiofur sodium [cef3, iv]). blood samples were collected for up to 196 hours. concentrations o ... | 2016 | 27227504 |
| utility of ultrasonography for diagnosis of superficial swellings in buffalo (bubalus bubalis). | we studied 72 buffalo with superficial swellings in the head (n=4), neck (n=5), chest wall (n=4), abdominal wall (n=28), limbs (n=16), gluteal region (n=8), perineal region (n=6) and udder (n=1). ultrasonographically, the swellings varied according to type, duration, content and location. the clinical use of ultrasound to assess these superficial swellings allowed diagnosis of abscesses (n=21), hematomas (n=11), hernias (n=17), bursitis (n=13), urethral diverticula (n=6) and tumors (n=4). ultras ... | 2016 | 27181085 |
| saliva ferning, an unorthodox estrus detection method in water buffaloes (bubalus bubalis). | estrus detection is a major problem in buffalo husbandry because of inconsistent expression of estrous signs at different seasons, and a high prevalence of the silent heat and postpartum anestrus in this species. around 50% of the estrus events in buffaloes are currently undetected in the field conditions, resulting in a huge economic loss. although the cervicovaginal fluid fern patterns confirm the estrus for a breeding decision, the fluid discharge is absent during the silent-heat condition. t ... | 2016 | 27181055 |
| impact of buserelin acetate or hcg administration on the day of first artificial insemination on subsequent luteal profile and conception rate in murrah buffalo (bubalus bubalis). | this study was designed to investigate the impact of buserelin acetate (ba) or human chorionic gonadotropin (hcg) administration on the day of first artificial insemination (ai) on subsequent luteal profile (diameter of corpus luteum (cl) and plasma progesterone) and conception rate in murrah buffalo. the present experiment was carried out at two locations in 117 buffalo that were oestrus-synchronized using cloprostenol (500 μg) administered (i.m.) 11 days apart followed by ai during standing oe ... | 2016 | 27170495 |
| strategies to overcome seasonal anestrus in water buffalo. | reproductive seasonality in buffalo (bubalus bubalis) is characterized by behavioral, endocrine, and reproductive changes that occur over distinct periods of the year. during the nonbreeding season (spring and summer), the greater light-dark ratio (long days) suppresses estrus behavior and the occurrence of ovulation. anestrous buffaloes have insufficient pulsatile of lh to support the final stages of follicular development, and subsequently, estrus behavior and ovulation do not occur, limiting ... | 2016 | 27157389 |
| effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (bubalus bubalis) bull spermatozoa. | the effects of equilibration times (e1, 2 h; e2, 4 h; e3, 6 h), freezing rates (fr1, manual, 5 cm above liquid nitrogen (ln2 ) for 10 min, plunging in ln2 ; fr2, programmable ultra-fast, holding at +4 °c for 2 min, from 4 to -10 °c at -10 °c/min, from -10 to -20 °c at -15 °c/min, from -20 to -120 °c at -60 °c/min, holding at -120 °c for 30 sec, plunging in ln2 ), and thawing rates (t1, 37 °c for 30 sec; t2, 50 °c for 15 sec; t3, 70 °c for 7 sec) were evaluated on quality of buffalo bull spermato ... | 2016 | 27153390 |
| embryonic and fetal mortality in river buffalo (bubalus bubalis). | river buffalo are able to adapt to diverse climatic zones and are widespread globally. the resource use efficiency of buffalo is highly relevant in a resource-constrained world and the increasing requirement to produce more food. buffalo clearly have an important role in meeting the growing demand for animal protein. in the mediterranean and higher latitudes, buffalo show annual cycles of ovarian activity, embryonic development, and pregnancy rate. in buffalo, the cl starts to develop early in t ... | 2016 | 27142486 |
| water buffalo (bubalus bubalis) as a spontaneous animal model of vitiligo. | vitiligo is a multifactorial acquired depigmenting disorder. recent insights into the molecular mechanisms driving the gradual destruction of melanocytes in vitiligo will likely lead to the discovery of novel therapies, which need to be evaluated in animal models that closely recapitulate the pathogenesis of human vitiligo. in humans, vitiligo is characterized by a spontaneous loss of functional melanocytes from the epidermis, but most animal models of vitiligo are either inducible or geneticall ... | 2016 | 27124831 |
| microorganisms in the rumen and reticulum of buffalo (bubalus bubalis) fed two different feeding systems. | the community of microorganisms in the rumen and reticulum is influenced by feeding as well as the species and geographical distribution of ruminant animals. bacteria, methanogenic archaea and ciliate protozoa existing in the rumen and reticulum were evaluated by real-time polymerase chain reaction and light microscopy in buffalo in two feeding systems, grazing and feedlot. | 2016 | 27118203 |
| flow cytometric and near-infrared raman spectroscopic investigation of quality in stained, sorted, and frozen-thawed buffalo sperm. | flow cytometry and laser tweezers raman spectroscopy have been used to investigate nili-ravi buffalo (bubalus bubalis) sperm from different samples (fresh, stained, sorted and frozen-thawed) of the flow-sorting process to optimize sperm sex sorting procedures. during the sorting and freezing-thawing processes, the two detection methods both indicated there were differences in mitochondrial activity and membrane integrity. moreover, a dispersive-type nir (near infrared reflection) use of the rama ... | 2016 | 27095613 |
| anti-mullerian hormone (amh) concentration in follicular fluid and mrna expression of amh receptor type ii and lh receptor in granulosa cells as predictive markers of good buffalo (bubalus bubalis) donors. | high individual variability in follicular recruitment and hence in the number of embryos produced is a major factor limiting the application of reproductive technologies in buffalo. therefore, the identification of reliable markers to select embryo donors is critical to enroll buffaloes in embryo production programs. better understanding of factors involved in follicular growth is also necessary to improve the response to superovulation in this species. the aim of this work was thus to determine ... | 2016 | 27087535 |
| understanding tenderness variability and ageing changes in buffalo meat: biochemical, ultrastructural and proteome characterization. | understanding of biological impact of proteome profile on meat quality is vital for developing different approaches to improve meat quality. present study was conducted to unravel the differences in biochemical, ultrastructural and proteome profile of longissimus dorsi muscle between buffaloes (bubalus bubalis) of different age groups (young v. old). higher (p<0.05) myofibrillar and total protein extractability, muscle fibre diameter, and warner-bratzler shear force (wbsf) values was observed in ... | 2016 | 27076348 |
| ultrastructural changes in the sublingual salivary gland of prenatal buffalo (bubalus bubalis). | the present study was aimed to elucidate ultrastructural changes in the development of sublingual salivary gland of buffalo during prenatal life. | 2016 | 27057120 |
| effect of incubation on freezability of cholesterol-loaded cyclodextrin treated buffalo (bubalus bubalis) spermatozoa. | the aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (clc) treated buffalo spermatozoa. | 2016 | 27051205 |
| effect of age, sex and physiological stages on hematological indices of banni buffalo (bubalus bubalis). | to determine the physiological baseline values for hematological indices of banni buffalo (bubalus bubalis) as well as to assess their alteration due to age, sex and physiological stages. | 2016 | 27051182 |
| freezability of water buffalo bull (bubalus bubalis) spermatozoa is improved with the addition of curcumin (diferuoyl methane) in semen extender. | effects of curcumin as antioxidant in extender were evaluated on freezability of buffalo spermatozoa. semen from each of the five bulls (n = 3 replicates, six ejaculates/bull, a total of 30 ejaculates) was diluted in tris-citric acid extender containing curcumin (0.5, 1.0, 1.5 or 2.0 mm) or control. at pre-freezing and post-thawing, total antioxidant contents (μm/l) and lipid peroxidation levels (μm/ml) were higher (p < .05) and lower (p < .05) respectively, with 1.5 and 2.0 mm compared to 0.5 a ... | 2016 | 27709643 |
| polymorphisms in the dgat1 gene in buffaloes (bubalus bubalis) in the amazon. | water buffaloes (bubalus bubalis) are quite well adapted to climatic conditions in the amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. because of a lack of research dedicated to improving the rearing of buffaloes in the amazon, the objective of this study was to genetically characterize the murrah and mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol o-acy ... | 2016 | 27706739 |
| effect of sex of embryo on developmental competence, epigenetic status, and gene expression in buffalo (bubalus bubalis) embryos produced by hand-made cloning. | buffalo embryos were produced by hand-made cloning using skin fibroblasts from male and female buffaloes (n = 4 each) as donor cells for examining the effect of sex. although the rate of blastocyst formation (43.8% ± 1.31% vs. 42.2% ± 1.22%) was similar, the total cell number (333 ± 10.4 vs. 270 ± 10.9) was higher (p < 0.05) whereas the apoptotic index (6.39 ± 0.25 vs. 8.52 ± 0.38) was lower (p < 0.05) for male than for female blastocysts. in the blastocysts, the global level of h3k18ac was foun ... | 2016 | 27696910 |
| complete genome sequence of a myoviridae bacteriophage infecting salmonella enterica serovar typhimurium. | the bacteriophage 118970_sal3 was isolated from water buffalo feces in southern italy, exhibiting lytic activity against salmonella enterica serovar typhimurium. this bacteriophage belongs to the myoviridae family and has a 39,464-bp double-stranded dna (ds-dna) genome containing 53 coding sequences (cdss). | 2016 | 27688333 |
| nutrient utilisation, growth performance and blood metabolites in murrah buffalo calves (bubalus bubalis) divergently selected for residual feed intake. | the aim of this study was to evaluate differences in efficiency of feed utilisation between buffalo calves with low and high residual feed intake (rfi) by comparing feed intake, nutrient digestibility, growth traits and blood metabolites. eighteen male murrah buffalo calves (aged 4-6 months; 70 ± 1.0 kg body weight) were fed ad libitum with a total mixed ration for 120 d. based on linear regression models involving dry matter intake (dmi), average daily gain (adg) and mid-test metabolic body siz ... | 2016 | 27666680 |
| developmental competence and expression profile of genes in buffalo (bubalus bubalis) oocytes and embryos collected under different environmental stress. | the study examined the effects of different environmental stress on developmental competence and the relative abundance (ra) of various gene transcripts in oocytes and embryos of buffalo. oocytes collected during cold period (cp) and hot period (hp) were matured, fertilized and cultured in vitro to blastocyst hatching stage. the mrna expression patterns of genes implicated in developmental competence (oct-4, igf-2r and gdf-9), heat shock (hsp-70.1), oxidative stress (mnsod), metabolism (glut-1), ... | 2016 | 27650183 |
| a comparative analysis of sperm selection procedures prior to cryopreservation for nili-ravi buffalo bull (bubalus bubalis) semen-: assessment of its impact on post-thaw sperm functional quality. | sperm selection techniques have been developed to get sperm suspensions enriched in motile and functional cells. studies show that selection before cryopreservation improves post-thaw quality of cryopreserved sperm but information on buffalo bull sperm is scarce. the study was aimed to 1) perform a comparative analysis of sperm selection procedures; swim-up (su), sephadex™-g15 filtration (s-g15) or glass wool filtration (gwf) for total and motile cell recovery, 2) to assess the impact of sperm s ... | 2016 | 27616354 |
| modelling lactation curve for milk fat to protein ratio in iranian buffaloes (bubalus bubalis) using non-linear mixed models. | the aim of this study was to compare seven non-linear mathematical models (brody, wood, dhanoa, sikka, nelder, rook and dijkstra) to examine their efficiency in describing the lactation curves for milk fat to protein ratio (fpr) in iranian buffaloes. data were 43 818 test-day records for fpr from the first three lactations of iranian buffaloes which were collected on 523 dairy herds in the period from 1996 to 2012 by the animal breeding center of iran. each model was fitted to monthly fpr record ... | 2016 | 27600968 |
| separation and quantification of milk casein from different buffalo breeds. | understanding the milk protein expression profile in different buffalo breeds plays an important role in improving hybrid selection and determining the effects on milk protein synthesis. the aim of this research is to compare the differences in milk protein content, composition and distribution between river buffalo and their crossbreeds for hybrid screening. four groups of milk samples that included nili-ravi (n), murrah (m), a nili-ravi-murrah crossbreed (m-n), and a crossbreed of river buffal ... | 2016 | 27600966 |
| relationship between the ovarian follicular response at the start of an ovsynch-tai program and pregnancy outcome in the mediterranean river buffalo. | the aim of this study was to evaluate the relationship between the ovarian follicular response at the start of the ovsynch-fixed-time artificial insemination (tai) program and pregnancy outcome in the mediterranean river buffalo. the study involved 116 pluriparous buffaloes at 118 ± 65 days in milk maintained under commercial conditions on a buffalo dairy enterprise in southern italy. synchronization of the stage of the estrous cycle in the ovsynch-tai program involves an injection of gnrh on da ... | 2016 | 27577986 |
| cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and dna integrities, and in vivo fertility of water buffalo (bubalus bubalis) spermatozoa. | the objective of the study was to devise a cryoprotection synergism between glycerol and dimethyl sulfoxide (dmso) for water buffalo spermatozoa. additionally, the effect of best evolved concentrations of glycerol and dmso in extender was assessed on in vivo fertility of buffalo spermatozoa. ejaculates (n = 30) were equally distributed into five aliquots; first aliquot was diluted at 37 °c in extender having 7 % glycerol (control); second aliquot was diluted at 37 °c as well as at 4 °c in extend ... | 2016 | 27761771 |
| programmable fast-freezing method improves the post-thaw motion dynamics, integrities of plasmalemma, mitochondrial transmembrane, dna and, acrosome, and in vivo fertility of water buffalo (bubalus bubalis) spermatozoa. | the effects of freezing methods (fr1, nonprogrammable/static, 5 cm above liquid nitrogen [ln2 ] for 10 min, plunging in ln2 ; fr2, programmable medium, +4°c to -15°c at 3°c min(-1) , from -15 to -80°c at 10°c min(-1) and final holding for 1 min at -80°c, plunging in ln2 ; fr3, programmable fast, from initial holding at +4°c for 2 min, from +4°c to -20°c at 10°c min(-1) , from -20°c to -100°c at 30°c min(-1) , final holding for 1 min at -100°c and plunging in ln2 ) were assessed on post-thaw in v ... | 2016 | 27813131 |
| identification of four snps in lhb gene and their associations with sperm qualities of chinese buffaloes. | luteinizing hormone beta polypeptide (lhb) gene has been considered important for sexual behavior and has associations with sperm quality. in this study, four snps (g.276 t>c, g.377a>c, g.401t>c, and g.412a>g) were detected in the lhb gene of 165 water buffaloes by direct sequencing and identification of overlap peaks, each of which was associated with at least one sperm quality trait of ejaculate volume, sperm concentration, post-thaw sperm motilities, and sperm abnormalities by chi-square anal ... | 2016 | 27854152 |
| effect of terminalia arjuna bark powder on some diagnostic enzymes in buffalo (bubalus bubalis) ingesting arsenic contaminated water and fodder. | the study investigated the effect of terminalia arjuna bark powder on some diagnostic enzymes related to hepatic and muscle function in buffaloes ingesting arsenic contaminated water and fodder in an arsenic affected area. | 2016 | 27847431 |
| seasonal variations of italian mediterranean buffalo (bubalus bubalis) mozzarella cheese quality. | water buffalo mozzarella cheese is more appreciated in the summer, but milk production is higher during wintertime, as water buffalo are seasonally polyoestrous animals. the aim of the study reported in this research communication was to evaluate the effect of the reversing of the calving period on mozzarella cheese composition and quality traits. ten batches of mozzarella cheeses produced during the summer and winter periods were analysed for ph, colour, chemical composition. seasonal differenc ... | 2016 | 27845021 |
| corrigendum to "investigation into omocysteine, vitamin e and malondialdehyde as indicators of successful artificial insemination in synchronized buffalo cows (bubalus bubalis)" [res. vet. sci. volume 104, february 2016, pages 100-105]. | 2016 | 27495365 | |
| random regression models to estimate genetic parameters for weights in murrah buffaloes. | this article reports genetic analysis of the weight at different ages of murrah water buffaloes, using random regression models (rrm). models ranging from third to sixth order polynomial were used to describe direct genetic and animal permanent environmental effects. contemporary group was included as a fixed effect, and a cubic polynomial was used to model the mean curve of the population. the residual was modeled considering a log-linear function. two models were selected for study of genetic ... | 2016 | 28004872 |
| efficiency of uterine fluid cytology in the diagnosis of subclinical endometritis in the water buffalo (bubalus bubalis). | this study compared endometrial cytology vis-a-vis uterine fluid cytology for assessment of uterine health in clinically normal and subclinical endometritis (se)-affected buffaloes. uterine fluid samples and endometrial samples were collected from the buffaloes (n = 38) at oestrus using blue sheath and cytobrush, respectively. the smears were stained with field stain for 3 minutes, and a minimum of 400 cells were counted in each smear for determination of the percentage of polymorphonuclear (pmn ... | 2016 | 27966806 |
| genetic diversity analysis of buffalo fatty acid synthase (fasn) gene and its differential expression among bovines. | fatty acid synthase (fasn) gene seems to be structurally and functionally different in bovines in view of their distinctive fatty acid synthesis process. structural variation and differential expression of fasn gene is reported in buffalo (bubalus bubalis), a bovine species close to cattle, in this study. amino acid sequence and phylogenetic analysis of functionally important thioesterase (te) domain of fasn revealed its conserved nature across mammals. amino acid residues at te domain, responsi ... | 2016 | 26376068 |
| replacement of serum with ocular fluid for cryopreservation of immature testes. | cryopreservation of immature testis is a feasible approach for germplasm preservation of male animals. combinations of dimethyl sulfoxide (dmso) and foetal bovine serum (fbs) are used for testis cryopreservation. however, an alternative to fbs is needed, because fbs is expensive. buffalo ocular fluid (buof), a slaughter house by-product, could be an economical option. the objective of the present study was to assess whether buof can replace fbs for cryopreservation of immature mouse (mus musculu ... | 2016 | 27693391 |
| magnetic resonance imaging and computer tomography of brain lesions in water buffaloes and cattle stunned with handguns or captive bolts. | owing to the demand for genuine mozzarella, some 330 water buffaloes are being slaughtered every year in switzerland albeit a stunning procedure meeting animal welfare and occupational safety requirements remains to be established. to provide a basis for improvements, we sized anatomical specifics in water buffaloes and cattle and we assessed brain lesions after stunning with captive bolts or handguns by diagnostic imaging. in water buffaloes and cattle, the median distance from the frontal skin ... | 2016 | 26610289 |
| loss of heat shock protein 70 from apical region of buffalo (bubalus bubalis) sperm head after freezing and thawing. | the post-thaw fertility of frozen-thawed mammalian spermatozoa is substantially low as compared with that of fresh sperm. furthermore, the post-thaw fertility of the cryopreserved buffalo sperm has been reported to be poor as compared with that of cattle sperm. recently, heat shock protein 70 (hsp70) has been found to play a critical role in mammalian fertilization and early embryonic development in boar and cattle. however, the presence of such fertility-related hsp70 in buffalo sperm and its s ... | 2016 | 26607876 |
| profile of muscle tissue gene expression specific to water buffalo: comparison with domestic cattle by genome array. | in contrast with the past, the water buffalo is now not only a draft animal, but also an important food source of milk and meat. it is increasingly apparent that the water buffalo have huge potential for meat production, but its breeding needs to be investigated. regarding the molecular mechanisms involved in the meat quality difference between the buffalo (bubalus bulabis) and yellow cattle (bos taurus), 12 chemical-physical characteristics related to the meat quality of longissimus thoracis mu ... | 2016 | 26598327 |
| molecular epidemiology of pathogenic leptospira spp. among large ruminants in the philippines. | the extent of leptospira infection in large ruminants resulting to economic problems in livestock industry in a leptospirosis-endemic country like the philippines has not been extensively explored. therefore, we determined the prevalence and carrier status of leptospirosis in large ruminants using molecular techniques and assessed the risk factors of acquiring leptospirosis in these animals. water buffalo and cattle urine samples (n=831) collected from 21 farms during 2013-2015 were subjected to ... | 2016 | 27452879 |
| a novel selection signature in stearoyl-coenzyme a desaturase (scd) gene for enhanced milk fat content in bubalus bubalis. | modern molecular interventions are dynamic gears for breeding animals with superior genetic make-up. these scientific efforts lead us toward sustainable dairy herds with improved milk production in terms of yield and quality. many of candidate genes have been dissected at molecular level, and suitable genetic markers have been identified in cattle, but this work has not been validated in buffaloes so far. stearoyl-coenzyme a desaturase (scd) has been a potential candidate gene for fat content of ... | 2016 | 27307277 |
| simultaneously tracing the geographical origin and presence of bovine milk in italian water buffalo mozzarella cheese using maldi-tof data of casein signature peptides. | water buffalo (wb) casein (cn) and curd samples from indigenous italian and international breeds were examined with the objective of identifying signature peptides that could function as an indicator to determine the origin of their milk products. cn in complex mixtures were digested with trypsin, and peptide fragments were subsequently identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms). the unique presence of a β-cn a variant and an interna ... | 2016 | 27299776 |
| assessment of foot health and animal welfare: clinical findings in 229 dairy mediterranean buffaloes (bubalus bubalis) affected by foot disorders. | lameness represents the third most important health-related cause of economic loss in the dairy industry after fertility and mastitis. although, dairy mediterranean buffaloes (mb) and dairy cows share similar breeding systems predisposing to similar herd problems, published studies exploring its relevance and role in these ruminants are still rare and incomplete. the aims of this study were to describe the clinical findings of foot disorders (fds) in dairy mb and their influence on animal welfar ... | 2016 | 27297174 |
| sister chromatid exchange test in river buffalo lymphocytes treated in vitro with furocoumarin extracts. | furocoumarin extracts from psoralea morisiana, the endemic sardinian legume species, were tested for their mutagenic potential on river buffalo blood cells. the results obtained performing the sister chromatid exchange (sce) test in blood cultures of five river buffalo calves (exposure to furocoumarins for 72h) and five cows (exposure to furocoumarins for 3h, in the absence and presence of s9 metabolic activator) are reported. significant differences in mean values of sces were observed in cells ... | 2016 | 27180332 |
| effect of mastitis on luteal function and pregnancy rates in buffaloes. | the aim of this study was to investigate the effects of mastitis on cl development and function and pregnancy rate in buffaloes. sixty-six buffaloes (bubalus bubalus) reared in a commercial farm at el-beheira governorate, north of egypt were used in this study. according to the visual observation of milk, physical examination of the udder and actual somatic cell count in milk, buffalo cows were divided into three groups: without mastitis (w), n = 23; subclinical mastitis (sc), n = 18; and clinic ... | 2016 | 27177967 |
| high genetic diversity and distribution of bubu-dqa alleles in swamp buffaloes (bubalus bubalis carabanesis): identification of new bubu-dqa loci and haplotypes. | in this study, genetic diversity analysis of mhc class ii-dqa locus helped in identification of 25 new bubu-dqa nucleotide sequences in swamp buffaloes (bubalus bubalis carabanesis, bubu). phylogenetic analysis revealed the distribution of the buffalo dqa sequences in two major clusters of dqa1 and dqa2 genes, sharing common lineages with corresponding cattle alleles, possibly due to trans-species evolution. however, a highly divergent sequence, bubu-dqa*2501, homologous to cattle (bola) dqa3 al ... | 2016 | 27177904 |
| polymorphism in the a2m gene associated with high-quality milk in murrah buffaloes (bubalus bubalis). | the study of genes associated with host defense mechanisms, such as the a2m gene, plays a critical role in preventing diseases that reduce milk yield and its constituents. the aim of this study was to identify polymorphisms in the a2m gene in murrah buffaloes (bubalus bubalis), and investigate their associations with milk yield, fat and protein production, fat and protein percentages, and somatic cell count. hair follicle samples of 136 animals were collected for dna extraction, and polymorphism ... | 2016 | 27173184 |
| expression of putative stem cell marker, hepatocyte nuclear factor 4 alpha, in mammary gland of water buffalo. | buffaloes account for more than 56% of total milk production in india. cyclic remodeling of mammary glands of human, mice, cow, sheep, and goat is determined by mammary stem cells. it is logical to assume that buffalo mammary gland will have mammary stem/progenitor cells. thus far, no report exists on identification of buffalo mammary stem cells. hepatocyte nuclear factor 4 alpha (hnf4a) is a candidate marker for hepatic progenitor cells and has recently been suggested as a marker of bovine mamm ... | 2016 | 27092988 |