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growing old with cystic fibrosis - the characteristics of long-term survivors of cystic fibrosis.the proportion of patients with cystic fibrosis (cf) who are middle-aged is increasing - and is likely to continue to do so. we surveyed a population of long-term cf survivors to assess their burden of illness and profile their disease characteristics.200919022643
viable ultramicrocells in drinking water.to examine the diversity of cultivable 0.2 micron filtrate biofilm forming bacteria from drinking water systems.200919040704
in vitro activities of a novel nanoemulsion against burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species.respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (cf). species within the burkholderia cepacia complex are especially problematic in this patient population. we investigated a novel surfactant-stabilized oil-in-water nanoemulsion (nb-401) for activity against 150 bacterial isolates recovered primarily from cf respiratory tract specimens. these specimens includ ...200918955531
sec- and tat-dependent translocation of beta-lactamases across the escherichia coli inner membrane.beta-lactamases represent the major resistance mechanism of gram-negative bacteria against beta-lactam antibiotics. the amino acid sequences of these proteins vary widely, but all are located in the periplasm of bacteria. in this study, we investigated the translocation mechanism of representative beta-lactamases in an escherichia coli model. n-terminal signal sequence analyses, antibiotic activity assay, and direct measurement of translocation of a green fluorescent protein (gfp) reporter fused ...200918981261
genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees.the association of endophytic bacteria with their plant hosts has a beneficial effect for many different plant species. our goal is to identify endophytic bacteria that improve the biomass production and the carbon sequestration potential of poplar trees (populus spp.) when grown in marginal soil and to gain an insight in the mechanisms underlying plant growth promotion. members of the gammaproteobacteria dominated a collection of 78 bacterial endophytes isolated from poplar and willow trees. as ...200919060168
stenotrophomonas maltophilia infections of intact skin: a systematic review of the literature.to highlight the importance of intact skin infection syndromes caused by stenotrophomonas maltophilia, we review 17 reported cases. skin infection syndrome presentations included metastatic cellulitis (58%), primary cellulitis (23%), and ecthyma gangrenosum (17%). associated risk factors were hematologic malignancies and chemotherapy (94%), neutropenia (94%), presence of central venous catheter (17%), and exposure to broad-spectrum antibiotics (84%). the diagnosis was supported by cultures of sk ...200919070451
the role of ampr in regulation of l1 and l2 beta-lactamases in stenotrophomonas maltophilia.stenotrophomonas maltophilia is known to produce at least two chromosomal-mediated inducible beta-lactamases, l1 and l2. gene l2, which encodes a class a beta-lactamase, and the adjacent ampr gene form an ampr-class a beta-lactamase module. l1 belongs to the class b beta-lactamase and has no neighbor ampr-like regulatory gene. in this study, the ampr-l2 module from s. maltophilia kh was compared with ampr-beta-lactamase modules from several microorganisms with respect to the ampr and beta-lactam ...200919071216
morphological and genetic analysis of three bacteriophages of serratia marcescens isolated from environmental water.increases in multidrug-resistant strains of serratia marcescens are of great concern in pediatrics, especially in neonatal intensive care units. in the search for bacteriophages to control infectious diseases caused by multidrug-resistant s. marcescens, three phages (ksp20, ksp90, and ksp100) were isolated from environmental water and were characterized morphologically and genetically. ksp20 and ksp90 belonged to morphotype a1 of the family myoviridae, and ksp100 belonged to morphotype c3 of the ...200919087204
selenite resistant rhizobacteria stimulate seo(3) (2-) phytoextraction by brassica juncea in bioaugmented water-filtering artificial beds.selenium is a trace metalloid of global environmental concern. the boundary among its essentiality, deficiency, and toxicity is narrow and mainly depends on the chemical forms and concentrations in which this element occurs. different plant species-including brassica juncea-have been shown to play a significant role in se removal from soil as well as water bodies. furthermore, the interactions between such plants, showing natural capabilities of metal uptake and their rhizospheric microbial comm ...200919104867
probable trimethoprim/sulfamethoxazole-induced higher-level gait disorder and nocturnal delirium in an elderly man.to report a case of probable trimethoprim/sulfamethoxazole (tmp/smx)-induced higher-level gait disorder (hlgd) and nocturnal delirium in an elderly patient on high-dose oral therapy.200919109207
melanonychia caused by stenotrophomonas maltophilia. 200919120402
genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils.arsenic is known as a toxic metalloid, which primarily exists in inorganic form [as(iii) and as(v)] and can be transformed by microbial redox processes in the natural environment. as(iii) is much more toxic and mobile than as(v), hence microbial arsenic redox transformation has a major impact on arsenic toxicity and mobility which can greatly influence the human health. our main purpose was to investigate the distribution and diversity of microbial arsenite-resistant species in three different a ...200919128515
carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a canadian mill.forty-one bacterial strains isolated from the headbox water of two machines in a canadian paper mill were associated with the genera asticcacaulis, acidovorax, bacillus, exiguobacterium, hydrogenophaga, pseudomonas, pseudoxanthomonas, staphylococcus, stenotrophomonas based on the sequence of their 16s rrna genes. the metabolic profile of these strains were determined using biolog ecoplate, and the bacteria were divided into four metabolic groups. metabolic profiles of the bacterial communities c ...200919137341
characterization of a novel stenotrophomonas isolate with high keratinase activity and purification of the enzyme.a feather-degrading bacterium was isolated from poultry decomposition feathers in china. the strain, named l1, showed significant feather-degrading activity because it grew and reproduced quickly on basal medium containing 10 g/l of native feather as the source of energy, carbon, and nitrogen. according to the phenotypic characteristics and 16s rrna profile, the isolate belongs to stenotrophomonas maltophilia. keratinase activity of the isolate was determined during cultivation on raw feathers a ...200919137342
investigation of bacterial pathogens on 70 frequently used environmental surfaces in a large urban u.s. university.after reports of increased severity of bacterial infections from community institutions, a broad spectrum of 70 surfaces was sampled for potential bacterial pathogens in the morning and afternoon of one day per week over three consecutive weeks in a large u.s. university. surfaces included public telephone mouthpieces, water fountain drains, student computer keyboards and desks, and buttons on elevators, vending machines, and photocopiers. a total of 420 samples was obtained. bacterial counts we ...200919192740
current status of newer carbapenems.beta-lactam antibiotics represent the most commonly prescribed antibacterial agents. since many bacteria have developed resistance to older agents, new beta-lactams have been introduced constantly. in the late 1970s, a new class of exceptionally broad-spectrum beta-lactams, the carbapenems, was identified. the carbapenems, such as the most popular imipenem and meropenem, have the widest spectra of antibacterial activity of all the beta-lactams and provide excellent coverage of many gram-negative ...200919199922
characterization of the charge variants of l2 beta-lactamase in stenotrophomonas maltophilia.stenotrophomonas maltophilia kh has two acid beta-lactamases with isoelectric points (pis) of 4.6 and 5.4, and several basic beta-lactamases (pis >7.0) that produce a ladder-shaped pattern by ief. an isogenic l2 mutant, khl2(xyle), was constructed by gene replacement. from ief and native page zymograms of strains kh and khl2(xyle), it was demonstrated that the basic beta-lactamases and the acid beta-lactamase with pi 5.4 are encoded by the same l2 gene and that the active types of these l2 charg ...200919208880
stenotrophomonas chelatiphaga sp. nov., a new aerobic edta-degrading bacterium.a new ethylenediaminetetraacetic acid (edta)-utilizing gammaproteobacterial strain lpm-5(t) was isolated from municipal sewage sludge. aerobic, gram-negative, motile rods multiply by binary fission. neutrophilic and mesophilic, these are unable to grow in the presence of 3% nacl (w/v), and unable to reduce nitrate to nitrite, and are oxidase and catalase positive, but lipase negative. the major cellular fatty acids are c(i15:0), c(a15:0) and c(16:1w7c). the dominant phospholipids are phosphatidy ...200919216044
community-acquired stenotrophomonas maltophilia infections: a systematic review.stenotrophomonas maltophilia is a pathogen that causes infections mainly in immunocompromised patients. however, community-acquired s. maltophilia infections have been occasionally reported. the objective of this paper was to collect and evaluate the available published data referring to community-acquired s. maltophilia infections. we searched pubmed, the cochrane library, and scopus for articles providing data for patients with community-acquired s. maltophilia infections. eight case series an ...200919224257
structure and mechanism of copper- and nickel-substituted analogues of metallo-beta-lactamase l1.in an effort to further probe metal binding to metallo-beta-lactamase l1 (mbetal l1), cu- (cu-l1) and ni-substituted (ni-l1) l1 were prepared and characterized by kinetic and spectroscopic studies. cu-l1 bound 1.7 equiv of cu and small amounts of zn(ii) and fe. the epr spectrum of cu-l1 exhibited two overlapping, axial signals, indicative of type 2 sites with distinct affinities for cu(ii). both signals indicated multiple nitrogen ligands. despite the expected proximity of the cu(ii) ions, howev ...200919228020
species distribution and antimicrobial susceptibility of gram-negative aerobic bacteria in hospitalized cancer patients.nosocomial infections pose significant threats to hospitalized patients, especially the immunocompromised ones, such as cancer patients.200919228413
antimicrobial activity of three tick defensins and four mammalian cathelicidin-derived synthetic peptides against lyme disease spirochetes and bacteria isolated from the midgut.in this study, chemically synthesized tick defensins and cathelicidin-derived mammalian peptides were used to investigate the activity spectrum against borrelia garinii and symbiotic stenotrophomonas maltophila. synthetic tick defensins showed antimicrobial activity against staphylococcus aureus but not b. garinii and s. maltophila. mammalian peptides which have cationic property similar to tick defensins, showed antimicrobial activity similar to tick defensins. the antimicrobial peptides in tic ...200919229642
[microbiological diagnosis of bronchopulmonary colonization-infection in cystic fibrosis].cystic fibrosis (cf), a condition produced by mutations in the gene that encodes the cystic fibrosis transmembrane conductance regulator, is the most prevalent autosomal-recessive hereditary disease in caucasian populations. among other repercussions, this defect leads to an alteration of respiratory secretions and determines a predisposition for chronic bronchopulmonary colonization-infection, which is the main driver of the high morbidity and early mortality of cf patients. colonization by sta ...200919232782
pneumonia in solid organ recipients: spectrum of pathogens in 217 episodes.lower respiratory tract infection (lrti) remains a leading cause of morbidity and mortality after solid organ transplantation (sot).200919249559
bacterial contamination of cystic fibrosis clinics.respiratory pathogens from cf patients can contaminate inpatient settings, which may be associated with increased risk of patient-to-patient transmission. few data are available that assess the rate of bacterial contamination of outpatient settings. we determined the frequency of contamination of cf clinics and the effectiveness of alcohol-based disinfectants in reducing hand carriage of bacterial pathogens.200919250885
a stenotrophomonas maltophilia multilocus sequence typing scheme for inferring population structure.stenotrophomonas maltophilia is an opportunistic, highly resistant, and ubiquitous pathogen. strains have been assigned to genogroups using amplified fragment length polymorphism. hence, isolates of environmental and clinical origin predominate in different groups. a multilocus sequence typing (mlst) scheme was developed using a highly diverse selection of 70 strains of various ecological origins from seven countries on all continents including strains of the 10 previously defined genogroups. se ...200919251858
low rates of pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients.pseudomonas aeruginosa is an important cause of pulmonary infection in cystic fibrosis (cf). its correct identification ensures effective patient management and infection control strategies. however, little is known about how often cf sputum isolates are falsely identified as p. aeruginosa. we used p. aeruginosa-specific duplex real-time pcr assays to determine if 2,267 p. aeruginosa sputum isolates from 561 cf patients were correctly identified by 17 australian clinical microbiology laboratorie ...200919261796
evaluation of the vitek 2 cards for identification and antimicrobial susceptibility testing of non-glucose-fermenting gram-negative bacilli.we evaluated vitek 2 cards (ngnc and ast-gn10) for the accuracy of identification (id) and antimicrobial susceptibility testing (ast) of non-glucose-fermenting gram-negative bacilli (ngf-gnb). in a total of 201 strains, 190 strains (94.5%) were correctly identified, seven strains (3.5%)showed low discrimination, four strains (2.0%) had discrepancies, and no strain remained unidentified.reference ast of amikacin, aztreonam, cefepime, cefotaxime, ceftazidime, ciprofloxacin, imipenem,levofloxacin, ...200919343822
bacterial communities in a crude oil gathering and transferring system (china).bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ...200919351561
activity of levofloxacin and ciprofloxacin on biofilms and planktonic cells of stenotrophomonas maltophilia isolates from patients with device-associated infections.the aim of this work was to compare the in vitro effects of levofloxacin and ciprofloxacin on pre-formed biofilms and planktonic populations of stenotrophomonas maltophilia isolates from device-associated infections recovered over the period 2004-2008 at a university hospital in argentina. for planktonic susceptibility studies, the minimum inhibitory concentrations (mics) of fluoroquinolones were determined by the broth microdilution method. from the 32 isolates recovered, 84.4% were sensitive t ...200919362452
early- and late-onset pneumonia: is this still a useful classification?the choice of empirical treatment of nosocomial pneumonia in the intensive-care unit (icu) used to rely on the interval after the start of mechanical ventilation. nowadays, however, the question of whether in fact there is a difference in the distribution of causative pathogens is under debate. data from 308 icus from the german national nosocomial infection surveillance system, including information on relevant pathogens isolated in 11,285 cases of nosocomial pneumonia from 1997 to 2004, were u ...200919364852
[results of 4 years of microbiological testing of bronchoscopes].in the context of reducing endoscopy-related infectious risk and new national guidelines on microbiological samples from bronchoscopy, the results of a surveillance program set up in a hospital center were analyzed.200919367202
survey of gut colonisation with stenotrophomonas maltophilia among neonates. 200919380180
pseudomonas aeruginosa las quorum sensing autoinducer suppresses growth and biofilm production in legionella species.bacteria commonly communicate with each other by a cell-to-cell signalling mechanism known as quorum sensing (qs). recent studies have shown that the las qs autoinducer n-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-c(12)-hsl) of pseudomonas aeruginosa performs a variety of functions not only in intraspecies communication, but also in interspecies and interkingdom interactions. in this study, we report the effects of pseudomonas 3-oxo-c(12)-hsl on the growth and suppression of virulence factors ...200919383702
identification of lysine positive non-fermenting gram negative bacilli (stenotrophomonas maltophilia and burkholderia cepacia complex).the burkholderia cepacia complex (bcc) and stenotrophomonas maltophilia are closely related groups of non-fermenting gram-negative bacilli (nfgnbs) having a similar spectrum of infections ranging from superficial to deep-seated and disseminated infections. identification of these lysine decarboxylase-positive nfgnbs lags behind in most indian laboratories. a simplified identification scheme was devised for these two pathogens that allowed us to isolate them with an increasing frequency at our te ...200919384035
stenotrophomonas maltophilia: an emerging opportunist human pathogen.stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in the debilitated host. s maltophilia is not an inherently virulent pathogen, but its ability to colonise respiratory-tract epithelial cells and surfaces of medical devices makes it a ready coloniser of hospitalised patients. s maltophilia can cause blood-stream infections and pneumonia with considerable morbidity in immunosuppressed patients. management of infection is hampered by high-level intrinsic resistance to ...200919393961
volatile organic compounds: a potential direct long-distance mechanism for antagonistic action of fusarium oxysporum strain msa 35.fusarium oxysporum msa35 [wild-type (wt) strain] is an antagonistic fusarium that lives in association with a consortium of bacteria belonging to the genera serratia, achromobacter, bacillus and stenotrophomonas in an italian soil suppressive to fusarium wilt. typing experiments and virulence tests provided evidence that the f. oxysporum isolate when cured of the bacterial symbionts [the cured (cu) form], is pathogenic, causing wilt symptoms identical to those caused by f. oxysporum f. sp. lactu ...200919396945
[comparison between agar dilution and three other methods for determining the susceptibility of 228 clinical isolates of non-fermenting gram-negative rods].susceptibility testing for non-fermenting gram-negative rods (nfgnr) is problematic; valid methods are needed for this purpose.200919406523
analysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by dgge.a microbial consortium was obtained by enrichment culture of sea water samples collected from botan oil port in xiamen, china, using the persistent high concentration of a mixture of polycyclic aromatic hydrocarbons enrichment strategy. denaturing gradient gel electrophoresis (dgge) was used to investigate the bacterial composition and community dynamic changes based on pcr amplification of 16s rrna genes during batch culture enrichment. using the spray-plate method, three bacteria, designated a ...200919409577
ampdi is involved in expression of the chromosomal l1 and l2 beta-lactamases of stenotrophomonas maltophilia.two ampd homologues, ampd(i) and ampd(ii), of stenotrophomonas maltophilia have been cloned and analyzed. comparative genomic analysis revealed that the genomic context of the ampd(ii) genes is quite different, whereas that of the ampd(i) genes is more conserved in s. maltophilia strains. the ampd system of s. maltophilia is distinct from that of the enterobacteriaceae and pseudomonas aeruginosa in three respects. (i) ampd(i) of s. maltophilia is not encoded in an ampde operon, in contrast to wh ...200919414581
hydroxylation of thiacloprid by bacterium stenotrophomonas maltophilia cgmcc1.1788.chloropyridinyl neonicotinoid insecticides play a major role in crop protection and flea control on cats and dogs. imidacloprid, thiacloprid and acetamiprid have in common the 6-chloro-3-pyridinylmethyl group but differ in the nitroguanidine or cyanoamidine substituent on an acyclic or cyclic moiety. our previous study found that stenotrophomonas maltophilia cgmcc 1.1788 could hydroxylate imidacloprid to 5-hydroxy imidacloprid, and 5-hydroxy imidacloprid was easily converted to 10-19 times highe ...200919421875
bacterial contamination of patients' medical charts in a surgical ward and the intensive care unit: impact on nosocomial infections.the purpose of this study was to determine the degree of bacterial contamination of patients' files, and to compare the colonized bacteria between files from the surgical intensive care unit (icu) and the surgical ward at the wan fang hospital, taipei, taiwan.200919424563
stenotrophomonas maltophilia endogenous endophthalmitis: clinical presentation, sensitivity spectrum and management.stenotrophomonas maltophilia is gaining importance as a community-acquired pathogen, after becoming firmly established as a nosocomial pathogen. here we report a case of endogenous endophthalmitis due to s. maltophilia. antibiotic-susceptibility testing of the isolate was performed by the kirby-bauer disc diffusion method. the organism was resistant to aminoglycosides, imipenem, ticarcillin and cotrimoxazole and was sensitive to ceftazidime and chloramphenicol. the patient was successfully treat ...200919429764
control of pseudomonas aeruginosa and stenotrophomonas maltophilia contamination of microfiltered water dispensers with peracetic acid and hydrogen peroxide.the abilities of peracetic acid and hydrogen peroxide to remove or reduce pseudomonas aeruginosa and stenotrophomonas maltophilia in output water from microfiltered water dispensers (mwds) were investigated. two mwds were inoculated with strains of p. aeruginosa and s. maltophilia isolated from water. dispensers a and b were disinfected with 10% (v/v) peracetic acid (paa) and 3% (v/v) hydrogen peroxide (hp) respectively. each dispenser was disinfected three times at monthly intervals with contac ...200919439386
pediatric infective endocarditis: has staphylococcus aureus overtaken viridans group streptococci as the predominant etiological agent?viridans group streptococci (vgs) have traditionally been the most common etiological agents of infective endocarditis (ie). advances in cardiovascular surgery and the increasing use of long-term central venous catheters may have altered the epidemiology of pediatric ie.200819145264
comparison of pulsed-field gel electrophoresis and three rep-pcr methods for evaluating the genetic relatedness of stenotrophomonas maltophilia isolates.in this study, three facile repetitive-sequence pcr (rep-pcr) techniques have been compared with the pulsed-field gel electrophoresis (pfge) method for differentiating the genetic relatedness of clinical stenotrophomonas maltophilia isolates.200819146527
modified nitrocefin-edta method to differentially quantify the induced l1 and l2 beta-lactamases in stenotrophomonas maltophilia.to estimate the ethylene diamine tetraacetic acid (edta) concentration at which the l1 enzyme activity in the cell extracts of stenotrophomonas maltophilia can be mostly inhibited.200819146537
tigecycline for treatment of nosocomial-acquired pneumonia possibly caused by multi-drug resistant strains of stenotrophomonas maltophilia. 200819129079
[respiratory tract fluid microbiology in an intensive care unit].to know patterns of antimicrobial resistance of bacterial isolates from tracheal aspirates in an intensive care unit and to evaluate the cases of ventilator-associated pneumonia.200819133212
prevalence of tetracycline resistance genes in greek seawater habitats.the presence of selected tetracycline resistance (tcr) genes was studied in different greek seawater habitats, originated from wastewater treatment facilities, fishfarm, and coastal environments. the methods employed included assessment of the presence of twelve gene clusters by pcr, followed by hybridization with specific probes, in habitat extracted dna, tc(r) bacteria, and exogenous isolated plasmids conferring tcr. the direct dna-based analysis showed that tet(a) and tet(k) genes were detect ...200819107391
effects of electric polarization of indium tin oxide (ito) and polypyrrole on biofilm formation.the influence of electric polarization on primary adhesion and on biofilm formation was investigated. as substrata, indium tin oxide (ito) and polypyrrole coatings were used because of their electric conductivity. the materials were polarized from -600 mv to +600 mv, switching every 60 seconds. control was non-polarized substrata. primary adhesion under this regime was not strongly influenced, however, the morphology of the primary biofilm was obviously different from that of the control. biofil ...200819092192
lung infections after cancer chemotherapy.lung infections can be severe consequences of chemotherapy-induced immune defects. aetiological causes of infection include bacteria (most commonly pseudomonas aeruginosa, stenotrophomonas maltophilia, and nocardia species), viruses (eg, respiratory syncytial virus, parainfluenza virus, influenza virus a and b, and cytomegalovirus), and fungi (eg, aspergillus, fusarium, and mucorales species, and pneumocystis jirovecii). most infections are caused by bacteria (especially gram negative), but viru ...200819071255
[the changing patterns and the associated factors of microbial pathogens in ventilator-associated pneumonia in a respiratory intensive care unit from 1995 to 2004].to investigate the changing patterns and associated factors of microbial pathogens which caused ventilator-associated pneumonia (vap) in our respiratory intensive care unit (ricu) from 1995 to 2004.200819080406
opportunity knocks. 200819086123
study on the bacterial midgut microbiota associated to different brazilian populations of lutzomyia longipalpis (lutz & neiva) (diptera: psychodidae).the bacterial community associated with the midgut of three brazilian lutzomyia longipalpis (lutz & neiva) populations, two from endemic areas for visceral leishmaniasis (jacobina, bahia state and são luís, maranhão state) and one from a non-endemic area (lapinha cave, minas gerais state), was identified. five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. the species identification was based on molecular and traditional ba ...200819061048
cluster of stenotrophomonas maltophilia endocarditis after prosthetic valve replacement.early postoperative prosthetic valve endocarditis due to stenotrophomonas maltophilia was diagnosed in seven patients (two men) aged from 68 to 84 years (mean age 78.1 years) over a three-year period. all patients had undergone aortic valve replacement. s. maltophilia was isolated from at least two blood cultures per patient. four patients experienced cns embolic complications. three patients died. all patients were treated with ceftazidime, one in combination with amikacin, one with ciprofloxac ...200818988010
acquisition and evolution of plant pathogenesis-associated gene clusters and candidate determinants of tissue-specificity in xanthomonas.xanthomonas is a large genus of plant-associated and plant-pathogenic bacteria. collectively, members cause diseases on over 392 plant species. individually, they exhibit marked host- and tissue-specificity. the determinants of this specificity are unknown.200819043590
purification and characterization of cytoplasmic nad-dependent polypropylene glycol dehydrogenase from stenotrophomonas maltophilia.the oxidizing enzyme nad(+)-dependent polypropylene glycol dehydrogenase (ppg-dh) was purified to homogeneity from the cytoplasmic fraction of stenotrophomonas maltophilia grown on polypropylene glycol (diol type) 2000. the purified enzyme consisted of a homotetrameric protein (37 kda subunit) with a molecular mass of around 154 kda. the n-terminal amino acid sequence (25 residues) showed similarity to the sequences of nad(+)-dependent secondary alcohol dehydrogenases and nadh-dependent reductas ...200819054086
cross-utilization and expression of outer membrane receptor proteins for siderophore uptake by diamondback moth plutella xylostella (lepidoptera: plutellidae) gut bacteria.siderophore production by entomo- and phytopathogens, plus the cross-utilization of these siderophores and expression of outer membrane receptor proteins (omrps) by diamondback moth (dbm) gut bacterial strains, were all examined. all the tested strains grew in the presence of 2, 2'-dipyridyl, and the brachybacterium sp. psgb10, pseudomonas sp. prgb06, and serratia marcescens flgb16 strains were found to cross-utilize the siderophores of various entomopathogens, including bacillus thuringiensis. ...200819054090
[nationwide susceptibility surveillance of ciprofloxacin and various parenteral antimicrobials against bacteria isolated from patients with severe infections--third ciproxan injection special survey (2005)].we conducted 3 nationwide surveillance studies between 2001 and 2005 at 39 participating institutions throughout japan according to the special survey plan to investigate susceptibility to ciprofloxacin (cpfx) and various parenteral antimicrobials using clinical isolates from patients with severe infection during the reexamination period of parenteral cpfx. results of the first special survey (2001) were already reported in this journal. the current third special survey (2005) was conducted at 3 ...200819024645
pcr-based rapid genotyping of stenotrophomonas maltophilia isolates.all bacterial genomes contain repetitive sequences which are members of specific dna families. such repeats may occur as single units, or found clustered in multiple copies in a head-to-tail configuration at specific loci. the number of clustered units per locus is a strain-defining parameter. assessing the length variability of clusters of repeats is a versatile typing methodology known as multilocus variable number of tandem repeat analysis (mlva).200819025624
independent predictors for mortality in patients with positive stenotrophomonas maltophilia cultures.stenotrophomonas maltophilia is an emerging pathogen in nosocomial infections that may result in high mortality. s. maltophilia often present as part of a polymicrobial culture and it is not well established when treatment is indicated. we aimed to identify predictors of mortality in patients with positive cultures of s. maltophilia.200819037515
appearance of multidrug-resistant opportunistic bacteria on the gingiva during leukemia treatment.dentists generally recognize the importance of periodontal treatment in patients with leukemia, with the most attention paid to preventing the development of odontogenic infection. for physicians, the worst type of infection is one caused by multidrug-resistant bacteria. here, we report a patient with an abnormal increase in multidrug-resistant opportunistic bacteria in the gingiva during hematopoietic cell transplantation (hct).200818166109
[stenotrophomonas maltophilia and cancer]. 200818949155
isolation of new stenotrophomonas bacteriophages and genomic characterization of temperate phage s1.twenty-two phages that infect stenotrophomonas species were isolated through sewage enrichment and prophage induction. of them, s1, s3, and s4 were selected due to their wide host ranges compared to those of the other phages. s1 and s4 are temperate siphoviruses, while s3 is a virulent myovirus. the genomes of s3 and s4, about 33 and 200 kb, were resistant to restriction digestion. the lytic cycles lasted 30 min for s3 and about 75 min for s1 and s4. the burst size for s3 was 100 virions/cell, w ...200818952876
diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots.forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ...200818759227
pseudoxanthomonas sacheonensis sp. nov., isolated from btex-contaminated soil in korea, transfer of stenotrophomonas dokdonensis yoon et al. 2006 to the genus pseudoxanthomonas as pseudoxanthomonas dokdonensis comb. nov. and emended description of the genus pseudoxanthomonas.a gram-negative, strictly aerobic, rod-shaped bacterium, designated strain bd-c54(t), was isolated from btex (benzene, toluene, ethylbenzene and xylenes)-contaminated soil in sacheon, korea. growth of strain bd-c54(t) was observed at 15-35 degrees c (optimum 25-30 degrees c) and ph 6.0-9.5 (optimum ph 7.0-8.0). the predominant fatty acids were iso-c(15:0), iso-c(17:1)omega9c, iso-c(11:0) 3-oh, iso-c(16:0), iso-c(11:0) and iso-c(17:0). the strain contained large amounts of phosphatidylethanolamin ...200818768635
effect of bacterial interference on biofilm development by legionella pneumophila.in the ecology of legionella pneumophila a crucial role may be played by its relationship with the natural flora; thus we investigated the interactions between legionella and other aquatic bacteria, particularly within biofilms. among 80 aquatic bacteria screened for the production of bacteriocin-like substances (blss), 66.2% of them were active against l. pneumophila. the possible effect of some of these aquatic bacteria on the development and stability of l. pneumophila biofilms was studied. p ...200818769851
non-susceptibility trends among pseudomonas aeruginosa and other non-fermentative gram-negative bacteria from bacteraemias in the uk and ireland, 2001-06.pseudomonas and acinetobacter spp. are important opportunists, notorious for resistance. pseudomonas spp. are collected in the british society for antimicrobial chemotherapy (bsac) bacteraemia surveillance, with acinetobacter spp. and stenotrophomonas maltophilia well represented in the 'other gram-negatives' group.200818819980
dipeptidyl aminopeptidase iv from stenotrophomonas maltophilia exhibits activity against a substrate containing a 4-hydroxyproline residue.the crystal structure of dipeptidyl aminopeptidase iv from stenotrophomonas maltophilia was determined at 2.8-a resolution by the multiple isomorphous replacement method, using platinum and selenomethionine derivatives. the crystals belong to space group p4(3)2(1)2, with unit cell parameters a = b = 105.9 a and c = 161.9 a. dipeptidyl aminopeptidase iv is a homodimer, and the subunit structure is composed of two domains, namely, n-terminal beta-propeller and c-terminal catalytic domains. at the ...200818820015
cross-reaction of stenotrophomonas and xanthomonas species in a 23s rrna gene-directed pcr for detection of s. maltophilia. 200818842940
[diagnostic and therapeutic management of gram-negative infections].among gram negative bacteria, pseudomonas aeruginosa, the extended spectrum beta-lactamases (esbl)-producing strains, acinetobacter spp, in particular the multiresistant acinetobacter baumannii, and stenotrophomonas maltophilia are the most implicated micrororganisms in the ever more increasing problem of bacterial resistance. possible solutions have to be searched, on one hand, in the use of new drugs but, on the other hand, in the re-evaluation of those already available drugs, possibly consid ...200818843221
development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux.human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. we describe the construction and development of an active surveillance of pathogens (asp) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes.200818844996
[extended-spectrum beta-lactamases-producing stenotrophomonas maltophilia strains: ctx-m enzymes detection and virulence study].to study the beta-lactamases content of stenotrophomonas maltophilia strains and to evaluate the virulence potential of these strains with the in vivo caenorhabditis elegans model.200818848407
[oligonucleotide primers for detection and amplification of the emoa gene encoding bacterial ethylenediaminetetraacetate monooxygenase].a system of primers was designed on the basis of analysis of nucleotide sequences of the emoa gene encoding ethylenediaminetetraacetate (edta) monooxygenase, which are deposited in genbank. this system of primers makes it possible to obtain emoa gene fragments approximately 750 bp long for bacterial destructors of edta. polymerase chain reaction (pcr) of total dna isolated from enrichment and pure cultures showed that this system can be effectively used for detecting the emoa gene in representat ...200818924405
dgge with genomic dna: suitable for detection of numerically important organisms but not for identification of the most abundant organisms.identification of all important community members as well as of the numerically dominant members of a community are key aspects of microbial community analysis of bioreactor samples. a systematic study was conducted with artificial consortia to test whether denaturing gradient gel electrophoresis (dgge) is a reliable technique to obtain such community data under conditions where results would not be affected by differences in dna extraction efficiency from cells. a total of 27 consortia were est ...200818929384
clinical impact of the over-expression of efflux pump in nonfermentative gram-negative bacilli, development of efflux pump inhibitors.in this manuscript, we want to review the biochemical and genetic characteristics of the different efflux pumps involved in both intrinsic and acquired multiresistance in non-fermentative gram-negative bacteria such as pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia, as well as the regulation of their expression. moreover, the clinical impact of the over-expression of these efflux pumps and the investigation developed to define efflux pump inhibitors will be dis ...200818781925
chemotaxonomy of bacteria by comprehensive gc and gc-ms in electron impact and chemical ionisation mode.the analysis of the cellular lipidic fraction of bacteria is described. after hydrolysis and methylation, the fatty acid methyl esters (fames) are determined by 1-d gc using the sherlock midi bacteria identification system, by comprehensive gc (gc x gc) and by gc-ms in electron impact (ei) and positive chemical ionisation (pci) mode. with gc x gc, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. gc-ei-ms and gc-pci-ms w ...200818792008
evolution of antimicrobial susceptibility patterns of aerobic and facultative gram-negative bacilli causing intra-abdominal infections: results from the smart studies 2003-2007.study for monitoring antimicrobial resistance trends (smart) is an ongoing global antimicrobial surveillance program focused on clinical isolates from intra-abdominal infections (iai). the objective of this subanalysis was to assess the evolution of the antimicrobial susceptibility patterns among aerobic and facultative gram-negative bacilli (gnb) recovered over a 5-year period at our institution. we tested the in vitro activity of the antimicrobials, commonly used to treat iai, against consecut ...200818792817
predictive analysis of transmissible quinolone resistance indicates stenotrophomonas maltophilia as a potential source of a novel family of qnr determinants.predicting antibiotic resistance before it emerges at clinical settings constitutes a novel approach for preventing and fighting resistance of bacterial pathogens. to analyse the possibility that novel plasmid-encoded quinolone resistance determinants (qnr) can emerge and disseminate among bacterial pathogens, we searched the presence of those elements in nearly 1000 bacterial genomes and metagenomes.200818793450
antimicrobial activities of the essential oils of origanum onites l., origanum vulgare l. subspecies hirtum (link) ietswaart, satureja thymbra l., and thymus cilicicus boiss. & bal. growing wild in turkey.in the present study, four separate samples of origanum onites l., three separate samples of satureja thymbra l., origanum vulgare l. ssp. hirtum (link) ietswaart, and thymus cilicicus boiss. & bal. were collected from various regions of mugla, turkey. the essential oils of these plants were obtained by the hydrodistillation method. antimicrobial activities of the essential oils against microorganisms, including multiple antibiotic-resistant bacteria, were investigated using the disc diffusion m ...200818800908
antibacterial agents and heavy metal resistance in gram-negative bacteria isolated from seawater, shrimp and sediment in iskenderun bay, turkey.the aim of the present study was to determine the level of antibiotic resistance patterns and distribution of heavy metal resistance of bacterial isolates from seawater, sediment and shrimps, and to determine if there is a relationship between antibiotic and heavy metal resistance. we undertook studies in 2007 in the industrially polluted iskenderun bay, on the south coast of turkey. the resistance of 236 gram-negative bacterial isolates (49 from seawater, 90 from sediment and 97 from shrimp) to ...200818804847
trends in stenotrophomonas maltophilia bloodstream infection in relation to usage density of cephalosporins and carbapenems during 7 years. 200818808347
[stenotrophomonas maltophilia bacteremia after endoscopic retrograde cholangiopancreatography]. 200818547765
fourier transform infrared spectroscopy for rapid identification of nonfermenting gram-negative bacteria isolated from sputum samples from cystic fibrosis patients.the accurate and rapid identification of bacteria isolated from the respiratory tract of patients with cystic fibrosis (cf) is critical in epidemiological studies, during intrahospital outbreaks, for patient treatment, and for determination of therapeutic options. while the most common organisms isolated from sputum samples are pseudomonas aeruginosa, staphylococcus aureus, and haemophilus influenzae, in recent decades an increasing fraction of cf patients has been colonized by other nonfermenti ...200818550747
the emergence of stenotrophomonas maltophilia. 200818556278
prosthetic aortic valve endocarditis due to stenotrophomonas maltophilia complicated by subannular abscess.annular abscesses are serious complications of infectious native and prosthetic valve endocarditis. in this patient, we isolated stenotrophomonas maltophilia, a rare cause of subaortic abscess with high mortality/morbidity rates although virulent gram-positive cocci, s. aureus in particular, have been the most commonly isolated agents. we treated this case of endocarditis and the subannular abscess observed 1 year after the initial operation by aortic root replacement with resternotomy in additi ...200818557156
evaluation of a modified selective differential medium for the isolation of stenotrophomonas maltophilia.via medium for stenotrophomonas maltophilia was modified by substituting meropenem (16 mg/l) for imipenem. s. maltophilia grew from 40% of drains sampled within a hospital and surrounding locations in perth, western australia. the specificity of the new medium for s. maltophilia was 62%, and all contaminating bacteria were easily distinguishable by phenotypic tests and pcr.200818558447
stenotrophomonas maltophilia bacteremia after living donor liver transplantation: report of a case.stenotrophomonas maltophilia (previously named xanthomonas maltophilia) is an aerobic, nonfermentive, gram-negative bacillus that is widespread in the environment. it is considered to be an organism with a limited pathogenic potential, which is rarely capable of causing diseases in humans other than in those who are in an immunocompromised state. in this study, we outline the case of a patient with stenotrophomonas maltophilia bacteremia after living donor liver transplantation, which showed the ...200818560974
a meningitis case due to stenotrophomonas maltophilia and review of the literature.stenotrophomonas maltophilia (formerly xanthomonas maltophilia) is a gram-negative bacillus increasingly associated with serious nosocomial infections. here, we describe a 30-year-old male patient who developed meningitis associated with this organism after several neurosurgical procedures. a review of the literature revealed only 15 previous reports. most cases were associated with neurosurgical procedures. antimicrobial therapy is complicated by multiple drug resistance of the organism, and tr ...200818579427
the plant-associated bacterium stenotrophomonas rhizophila expresses a new enzyme for the synthesis of the compatible solute glucosylglycerol.the rhizobacterium stenotrophomonas rhizophila accumulates the compatible solutes glucosylglycerol (gg) and trehalose under salt stress conditions. the complete gene for the gg synthesis enzyme was cloned and sequenced. this enzyme from s. rhizophila represented a novel fusion protein composed of a putative c-terminal gg-phosphate synthase domain and an n-terminal putative gg-phosphate phosphatase domain, which was named ggpps. a similar gene was cloned from pseudomonas sp. strain oa146. the ggp ...200818586931
induction potential of clavulanic acid toward l1 and l2 beta-lactamases of stenotrophomonas maltophilia. 200818592278
microbial diversity of soil bacteria in agricultural field contaminated with heavy metals.in this study we evaluated the bacterial diversity in a soil sample from a site next to a chemical industrial factory previously contaminated with heavy metals. analysis of 16s rdna sequences amplified from dna directly extracted from the soil revealed 17 different bacterial types (genera and/or species). they included polyangium spp., sphingomonas spp., variovorax spp., hafina spp., clostridia, acidobacteria, the enterics and some uncultured strains. microbes able to tolerate high concentration ...200818595405
identification and management of unusual pathogens in cystic fibrosis. 200818607011
oil-removal enhancement in media with keratinous or chitinous wastes by hydrocarbon-degrading bacteria isolated from oil-polluted soils.the aim of this work was to isolate oil-degrading bacteria that use chitin or keratin as carbon sources from oil contaminated soils; and additionally to study if oil removal by these bacteria is enhanced when a chitinous or a keratinous waste is added to the culture media. to isolate the above-mentioned bacteria, 12 soil samples were collected close to an oil-well. such soils showed unsuitable nutrients content, but their counts of heterotrophic bacteria ranged within 10(5)-10(8) cfu g(-1) soil, ...200818613616
risk factors associated with mortality of infections caused by stenotrophomonas maltophilia: a systematic review.the objective of this review was to assess the quality of available literature regarding risk factors associated with mortality of infections caused by s. maltophilia. pubmed and ovid were searched from march 1985 to march 2008, and eligible studies were considered to be those that related s. maltophilia infection with risk factors associated with mortality; described the characteristics of patients in detail; and provided data regarding the outcome and mortality. thirty-eight studies were found ...200818621440
evidence for specificity of cultivable bacteria associated with arbuscular mycorrhizal fungal spores.bacteria associated with arbuscular mycorrhizal (am) fungal spores may play functional roles in interactions between am fungi, plant hosts and defence against plant pathogens. to study am fungal spore-associated bacteria (amb) with regard to diversity, source effects (am fungal species, plant host) and antagonistic properties, we isolated amb from surface-decontaminated spores of glomus intraradices and glomus mosseae extracted from field rhizospheres of festuca ovina and leucanthemum vulgare. a ...200818631178
smqnr, a new chromosome-carried quinolone resistance gene in stenotrophomonas maltophilia.a new chromosome-carried quinolone resistance gene from stenotrophomonas maltophilia, smqnr, was characterized. the gene was present in type strain ccug 5866 and was also detected in 24 clinical isolates and showed some allelic diversity. the expression of smqnr in escherichia coli decreased the susceptibilities of the e. coli isolates to several fluoroquinolones.200818644963
culturable aerobic bacteria from the upstream region of a karst water rivulet.the composition of 681 aerobic and heterotrophic strains that were isolated on two different media was assessed at four sampling points along a approximately 300 m stretch of a karst water rivulet. based on partial sequence analysis of 16s rrna genes, members of 35 genera were identified; however, only a few species dominated as their representatives were repeatedly isolated at different sampling sites. determination of the phylum affiliation showed that the isolates included members of bacterio ...200818645959
anti-adherence activity and antimicrobial durability of anti-infective-coated catheters against multidrug-resistant bacteria.to investigate the anti-adherence and antimicrobial durability of anti-infective catheters against multidrug-resistant (mdr) staphylococcus aureus (resistant to vancomycin, rifampicin and methicillin) and mdr gram-negative bacteria (stenotrophomonas maltophilia, acinetobacter baumannii/calcoaceticus and enterobacter agglomerans) that are often associated with catheter-related bloodstream infections (crbsis).200818653489
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