Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [morphofunctional changes in the midgut of the ixodes females (acari: ixodidae) during the immunizing feedings]. | the changes of the midgut in the females of the tick species ixodes persulcatus and i. ricinus during the second and third immunizing feeding on rabbits were studied by the histological technics. the alternation of one generation of the digestive cells of nymphal stage and two generations of the digestive cells of adult stage was observed. the generation of secretory cells is absent. the tick completes feeding and drop off when the last generation of the digestive cells is on the initial activit ... | 2006 | 17042279 |
| [the abundance and distribution of the ixodes persulcatus (acari: ixodidae) near its northern spreading limit in the ural mountains]. | a count of the tick species ixodes persulcatus schulze, 1930 was carried out in the "denezhkin kamen" nature reserve and adjacent territories (the severoural'sk and ivdel' districts of the sverdlovsk region, the northern urals geographical province) in the 2005. the abundance and distribution of unengorged adults has been evaluated on an area of 22.5 square kilometers (n 60 degrees 27'-60 degrees 30' e 059 degrees 38'-059 degrees 42'). the area includes proportionally main landscape and vegetati ... | 2006 | 17042282 |
| human granulocytic anaplasmosis and anaplasma phagocytophilum. | human granulocytic anaplasmosis is a tickborne rickettsial infection of neutrophils caused by anaplasma phagocytophilum. the human disease was first identified in 1990, although the pathogen was defined as a veterinary agent in 1932. since 1990, us cases have markedly increased, and infections are now recognized in europe. a high international seroprevalence suggests infection is widespread but unrecognized. the niche for a. phagocytophilum, the neutrophil, indicates that the pathogen has unique ... | 2005 | 16485466 |
| borrelia burgdorferi, host-derived proteases, and the blood-brain barrier. | neurological manifestations of lyme disease in humans are attributed in part to penetration of the blood-brain barrier (bbb) and invasion of the central nervous system (cns) by borrelia burgdorferi. however, how the spirochetes cross the bbb remains an unresolved issue. we examined the traversal of b. burgdorferi across the human bbb and systemic endothelial cell barriers using in vitro model systems constructed of human brain microvascular endothelial cells (bmec) and ea.hy 926, a human umbilic ... | 2005 | 15664945 |
| characterization of borrelia burgdorferi sensu lato isolated in moscow province--a sympatric region for ixodes ricinus and ixodes persulcatus. | the relationships between borrelia species and the vector ticks, ixodes persulcatus and ixodes ricinus were examined in a molecular epidemiological study. we conducted a survey in the moscow region which is a sympatric region for both species of tick. we examined 630 unfed i. ricinus and i. persulcatus, ticks collected from four different regions around moscow within an area of 250 km2. eighty-four ticks were culture positive (13.3%) and the prevalence rate varied in each region from 5.7% to 42. ... | 2005 | 15715174 |
| identification of a new borrelia species among small mammals in areas of northern spain where lyme disease is endemic. | the role of small mammals as reservoir hosts for borrelia burgdorferi was investigated in several areas where lyme disease is endemic in northern spain. a low rate of infestation by ixodes ricinus nymphs was found in the small mammal populations studied that correlated with the near-absence of b. burgdorferi sensu lato in 184 animals tested and with the lack of transmission of b. burgdorferi sensu lato to i. ricinus larvae that fed on them. in contrast, questing ticks collected at the same time ... | 2005 | 15746336 |
| detection and identification of ehrlichia spp. in ticks collected in tunisia and morocco. | a broad-range 16s rrna gene pcr assay followed by partial sequencing of the 16s rrna gene was used for the detection of members of the family anaplasmataceae in ticks in north africa. a total of 418 questing ixodes ricinus ticks collected in tunisia and morocco, as well as 188 rhipicephalus ticks from dogs and 52 hyalomma ticks from bovines in tunisia, were included in this study. of 324 adult i. ricinus ticks, 16.3% were positive for ehrlichia spp., whereas only 3.4 and 2.8% of nymphs and larva ... | 2005 | 15750072 |
| sequence analysis of the msp4 gene of anaplasma phagocytophilum strains. | the causative agent of human granulocytic ehrlichiosis was recently reclassified as anaplasma phagocytophilum, unifying previously described bacteria that cause disease in humans, horses, dogs, and ruminants. for the characterization of genetic heterogeneity in this species, the homologue of anaplasma marginale major surface protein 4 gene (msp4) was identified, and the coding region was pcr amplified and sequenced from a variety of sources, including 50 samples from the united states, germany, ... | 2005 | 15750101 |
| diversity of borrelia burgdorferi sensu lato in russian far east. | thirty strains of borrelia burgdorferi sensu lato have been isolated from ixodes persulcatus ticks and from skin lesions of lyme disease patients in the russian far east from 1997 to 2003. we amplified full-length outer surface protein a (ospa) gene of all strains. blast search and following phylogenetic analysis showed that strains form four well-defined groups. four strains belong to borrelia afzelii species. other strains distributed into tree major groups, identified as borrelia garinii. ind ... | 2005 | 15781992 |
| the microbial rosetta stone database: a compilation of global and emerging infectious microorganisms and bioterrorist threat agents. | thousands of different microorganisms affect the health, safety, and economic stability of populations. many different medical and governmental organizations have created lists of the pathogenic microorganisms relevant to their missions; however, the nomenclature for biological agents on these lists and pathogens described in the literature is inexact. this ambiguity can be a significant block to effective communication among the diverse communities that must deal with epidemics or bioterrorist ... | 2005 | 15850481 |
| molecular identification and analysis of borrelia burgdorferi sensu lato in lizards in the southeastern united states. | lyme borreliosis (lb) group spirochetes, collectively known as borrelia burgdorferi sensu lato, are distributed worldwide. wild rodents are acknowledged as the most important reservoir hosts. ixodes scapularis is the primary vector of b. burgdorferi sensu lato in the eastern united states, and in the southeastern united states, the larvae and nymphs mostly parasitize certain species of lizards. the primary aim of the present study was to determine whether wild lizards in the southeastern united ... | 2005 | 15870353 |
| immunoglobulin-regulated expression of borrelia burgdorferi outer surface protein a in vivo. | borrelia burgdorferi, the agent of lyme disease, down-regulates outer surface protein a (ospa), which is abundantly expressed in ticks, during infection of the mammalian host. in this study we examined the signals that may be responsible for maintaining the ospa-negative state of spirochetes during infection. transcription of ospa mrna was found in tissues of c3h-severe combined immunodeficient (c3h-scid) mice, but not immunocompetent c3h mice, inoculated with cultured b. burgdorferi, tick-borne ... | 2005 | 15908357 |
| [study on the coinfection of three tick-borne infectious diseases in china using polymerase chain reaction method]. | to study the existence of ehrluichiosis, lyme disease and tick-borne spotted fever coinfection in some areas in china. | 2005 | 15921584 |
| prevalence and incidence of antibodies to borrelia burgdorferi and to tick-borne encephalitis virus in agricultural and forestry workers from tuscany, italy. | the ticks ixodes persulcatus and ixodes ricinus are the main vectors of both borrelia burgdorferi sensu lato and tick-borne encephalitis (tbe) virus in eurasia. borrelia burgdorferi is the cause of lyme borreliosis, and tbe is a biphasic meningoencephalitis induced by an arbovirus belonging to the flavivirus family. the principal aims of the current investigation were (i) to determine the frequency of serological evidence of borrelia burgdorferi sensu lato and tbe infections in healthy agricultu ... | 2005 | 15948001 |
| innate immune responses in lyme borreliosis: enhanced tumour necrosis factor-alpha and interleukin-12 in asymptomatic individuals in response to live spirochetes. | innate immunity is important for early defence against borrelia spirochetes and should play a role in the clinical outcome of the infection. in order to study early cytokine responses, in vitro differentiated dendritic cells (dcs) and whole blood cells from 21 patients with different clinical outcomes of lyme neuroborreliosis were stimulated with live borrelia spirochetes. the borrelia-induced secretion of interleukin (il)-4, il-10, il-12p70, interferon (ifn)-gamma and tumour necrosis factor (tn ... | 2005 | 15958074 |
| [detection of a new species of rickettsiae in the ticks of ixodes persulcatus in russia]. | genetic analyses (polymerase chain reaction and nucleotide sequence test) have been used to describe a new species of rickettsiae in the ticks ipersukarus in russia. this species is named as rzckettsia tarasevichiae after academician of the russian academy of medical sciences irma vladimirovna tarasevich, head of the laboratory of rickettsial ecology, n.f.gamaleya research institute of epidemiology and microbiology. a total of 317 ipersukatus imagoes were collected in the regions of the urals, w ... | 2005 | 15984607 |
| possible tick-borne human enterovirus resulting in aseptic meningitis. | enterovirus-specific genetic sequences were isolated from two amblyomma americanum tick pools. identical genetic sequences were later obtained from cerebrospinal fluid of a patient with aseptic meningitis and a recent history of tick attachment. these observations suggest the possibility of an emerging tick-borne human enterovirus associated with aseptic meningitis. | 2005 | 16000481 |
| [monitoring of tick-borne encephalitis virus populations and etiological structure of morbidity over 60 years]. | the siberian subtype of the virus of tick-borne encephalitis (tbe), which is predominant in russia, constantly circulated in its eastern european regions in 1943-2003 and in the urals and west and east siberia in 1960-2003. this subtype is transmitted by two types of ticks: ixodes persulcatus and i. ricinis. changes were not found in the structure of viral populations at the peak and drop of the incidence of tbe. there was new evidence on the genetic heterogenicity of the siberian subtype: in ad ... | 2005 | 16078427 |
| [gradual variability of the parasitic system of tick-borne encephalitis]. | it has been shown that the temperature conditions of hibernation in the major vectors of tick-borne encephalitis viruses (tbev) the ticks ixodes ricinus and ixodes persulcatus occupy a special place among a lot of factors that determine the spatial distribution and the specific features of functioning of the parasitic system of tick-borne encephalitis. these conditions determine the borders of distribution of the major vectors of tbev, their sympatric zone, and the physiological features of indi ... | 2005 | 16078435 |
| babesia dna detection in canine blood and dermacentor reticulatus ticks in southwestern siberia, russia. | babesia infection was studied in 21 blood samples of dogs with symptoms of babesiosis and among 72 dermacentor reticulatus and 70 ixodes persulcatus ticks from southwestern siberia, russia. babesia dna was detected by hemi-nested pcr based on the 18s rrna gene with subsequent direct sequencing. all of the analyzed canine blood samples and three d. reticulatus, but none from i. persulcatus ticks studied were shown to contain babesia dna. nucleotide sequences of the babesia 18s rrna gene fragment ... | 2005 | 16187898 |
| tick-borne rickettsioses around the world: emerging diseases challenging old concepts. | during most of the 20th century, the epidemiology of tick-borne rickettsioses could be summarized as the occurrence of a single pathogenic rickettsia on each continent. an element of this paradigm suggested that the many other characterized and noncharacterized rickettsiae isolated from ticks were not pathogenic to humans. in this context, it was considered that relatively few tick-borne rickettsiae caused human disease. this concept was modified extensively from 1984 through 2005 by the identif ... | 2005 | 16223955 |
| effects of anaplasma phagocytophilum on host cell ferritin mrna and protein levels. | ferritin is a major intracellular iron storage protein and also functions as a cytoprotectant by sequestering iron to minimize the formation of reactive oxygen species. anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, is an obligate intracellular bacterium that colonizes neutrophils. we have previously reported that human promyelocytic hl-60 cells infected with a. phagocytophilum demonstrate increased transcription of ferritin heavy chain and also that the bacte ... | 2005 | 16239567 |
| [the peculiarities of the biology of ticks inhabiting the environs of tomsk city]. | the presence of ticks ixodes persulcatus and i. pavlovskyi occidentalis is revealed in urban parks and the forests directly adjoined to buildings. i. pavlovskyi occidentalis is found to be the dominant species nearby the city. the both species are occurred almost simultaneously, and the peak of their abundance usually falls on 2nd or 3rd ten-day period of may. in the city the ticks species ceased occurring in registers in the end of may, and in the adjacent forests they disappeared in the end of ... | 2005 | 16316054 |
| tickborne pathogen detection, western siberia, russia. | ixodes persulcatus (n = 125) and dermacentor reticulatus (n = 84) ticks from western siberia, russia, were tested for infection with borrelia, anaplasma/ehrlichia, bartonella, and babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. i. persulcatus ticks were infected with borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), anaplasma phagocytophilum (2.4% +/- 1.4%), ehrlichia muris (8.8% +/- 2.5%), and bartonella spp. (37.6% +/- 4.3%). d. re ... | 2005 | 16318722 |
| anaplasma phagocytophilum-infected ticks, japan. | we report anaplasma phagocytophilum infection of ixodes persulcatus and i. ovatus ticks in japan. unique p44/msp2 paralogs (and/or 16s rrna genes) were detected in tick tissues, salivary glands, and spleens of experimentally infected mice. these findings indicate the public health threat of anaplasmosis in japan. | 2005 | 16318739 |
| [ixodidae ticks in bishkek]. | the fauna of ixodidae ticks was studied in bishkek in 1998-2004. the city is situated in the center of the chuisk valley, at the foot of the kirghiz ridge, at the boundary of a submountain-plain-arid region and a plain-piedmont-semiarid one with steppe fragments. the ticks were gathered on a flag from small mammalians caught by gero traps, from birds, domestic and agricultural animals. a total of 648 animals of 13 species were examined. the bites of ticks were registered in 98 persons in the cit ... | 2005 | 16445237 |
| semi-nested pcr detection of bartonella henselae in ixodes persulcatus ticks from western siberia, russia. | questing adult ixodes persulcatus ticks from western siberia, russia were tested for infections with bartonella spp. using seminested pcr assay with primers specific to the groel gene. the proportion of ticks infected with bartonella spp. was 44% in 2002 (n = 50) and 38% in 2003 (n = 50). nucleotide sequences of a portion of the pcr products corresponded to bartonella henselae species. | 2004 | 15671737 |
| [possible types of tick-borne diseases and the predictive role of history data in their diagnosis (parasitological aspects of the problem)]. | the paper discusses the proven and probable role of different tick-borne pathogens and their combinations in the occurrence of zoonotic diseases. the variants of coexistence of various combinations of 7 proved disease agents (3 species of borrelia, 2 species of ehrlichia, babesia, and tbe virus) with different, if might to be, disease agents, such as rickettsiae of two groups, which do not belong to the borrelia burgdorferi sensu lato group spirochetes, are analyzed. the difference of their deve ... | 2004 | 15689134 |
| mechanisms of immunity to ehrlichia muris: a model of monocytotropic ehrlichiosis. | ehrlichia species can cause life-threatening infections or chronic persistent infections. mechanisms of protective immunity were examined in an ehrlichia muris mouse model of monocytotropic ehrlichiosis. c57bl/6 mice possessed strong genetic resistance to e. muris of an undetermined mechanism. cd8 t lymphocytes were particularly important, as revealed by 81% fatalities for e. muris-infected, major histocompatibility complex class i gene knockout mice compared with no deaths for wild-type c3h mic ... | 2004 | 14742542 |
| [the siberian and far-eastern subtypes of tick-borne encephalitis virus registered in russia's asian regions: genetic and antigen characteristics of the strains]. | agar gel precipitation test with cross-adsorbed immune sera was used for the antigenic differentiation of strains of tick-borne encephalitis virus (tbev). fifty strains of the far east tbev serotype and 46 strains of the siberian (aina) tbev serotype were isolated from ixodes persulcatus, which is the main vector of the above tbev subtypes in the asian and european parts of russia. the fragment of the envelope protein gene was sequenced for tbev strains. sequences of new-group strains of the sib ... | 2004 | 15293507 |
| comparison of disseminated and nondisseminated strains of borrelia burgdorferi sensu stricto in mice naturally infected by tick bite. | clinical isolates of borrelia burgdorferi sensu stricto have been categorized into disseminated and nondisseminated groups based on distinct ribosomal spacer restriction fragment length polymorphism genotypes (rsts). in order to determine whether transmission by tick bite would alter the dissemination dynamics and disease produced by distinct genotypes, disseminated isolates (rst1), nondisseminated isolates (rst3), and a standard laboratory strain (b-31) were established in a murine cycle utiliz ... | 2004 | 15322021 |
| a comparison of serologic tests for the detection of serum antibodies to whole-cell and recombinant borrelia burgdorferi antigens in cattle. | serum samples from healthy dairy and beef cattle, living in tick-infested areas of connecticut, usa, were analyzed by polyvalent enzyme-linked immunosorbent assays (elisa), indirect fluorescent antibody (ifa) staining methods, or western blot procedures to detect antibodies to tick-borne agents. of the 80 sera tested by elisa with whole-cell or 10 separate recombinant antigens (fusion proteins) of borrelia burgdorferi sensu stricto, 57 (71%) were positive to 1 or more antigens, while 36 (45%) re ... | 2004 | 15368740 |
| [population dynamics of ixodes persulcatus in a subzone of the south taiga of the middle urals]. | the results of a 27-year follow-up of the size of the taiga tick in a subarea of the south taiga of the middle urals are summarized. the author has established the period of its activity, the change in the parameter of the activity by years, and the rise in the size of the vector in the past decade. | 2004 | 15484981 |
| [fine structure of tarsal sensilla in the taiga tick ixodes persulcatus (ixodinae)]. | the first tarsal segment of the taiga tick bears 4 general types of sensilla (except for sensilla forming the haller's sensory organ on the dorsal surface of the tarsus): tactile mechanoreceptor sensilla of 3 types, contact chemo-mechanoreceptor sensilla of 2 types, and double-walled pore sensilla. one of these types, the chemo-mechanoreceptor upper-pore sensilla, was found only in the ixodinae, and not found in the examined representatives of the amblyomminae. this type of sensilla was also fou ... | 2004 | 15493282 |
| detection of anaplasma phagocytophilum dna in ixodes ticks (acari: ixodidae) from madeira island and setubal district, mainland portugal. | a total of 278 ixodes ticks, collected from madeira island and setubal district, mainland portugal, were examined by polymerase chain reaction (pcr) for the presence of anaplasma phagocytophilum. six (4%) of 142 ixodes ricinus nymphs collected in madeira island and 1 nymph and 1 male (2%) of 93 i. ventalloi collected in setubal district tested positive for a. phagocytophilum msp2 genes or rrs. infection was not detected among 43 i. ricinus on mainland portugal. all pcr products were confirmed by ... | 2004 | 15498168 |
| typing of borrelia relapsing fever group strains. | partial sequencing of the 16s-23s rdna intergenic spacer showed two to four genotypes each for borrelia hermsii and b. turicatae, both relapsing fever agents transmitted by argasid ticks, and for b. miyamotoi and b. lonestari, transmitted by ixodid ticks. field surveys of ixodes ticks in connecticut and sweden showed limited local diversity for b. miyamotoi. | 2004 | 15498172 |
| [genetic identification of rickettsiae of the tick-borne spotted fever group, isolated in the foci of tick-borne rickettsiosis]. | a total of 25 rickettsial cultures of the tick-borne spotted fever (tbsf) group from the collection of the research institute of infections in omsk, isolated from different sources in the territory of the russian federation (from the urals to the far east) during the period of 1954-2001) were studied by the methods of genetic analysis. the fragments of the gene coding the outer-membrane protein of 190 kd (ompa) and synthetase citrate (glta) of the rickettsiae under the study were sequenced. 23 i ... | 2004 | 15554312 |
| terrestrial distribution of the lyme borreliosis agent borrelia burgdorferi sensu lato in east asia. | lyme borreliosis is the most prevalent tick-borne zoonosis and an important emerging infection in europe, north america, and far eastern countries. the geographical distribution of borrelia spp. and the relationship between borrelia spp. and tick spp. in east asian countries have been studied. in northern asian countries, ixodes persulcatus carries eurasian-type borrelia garinii (20047 type), borrelia afzelii, and asian-type b. garinii (variant nt29), whereas borrelia burgdorferi sensu stricto h ... | 2004 | 15623946 |
| [methods for estimating the demographic structure of the taiga tick (ixodidae) based on results of standard parasitological observations]. | a retrospective estimation of the abundance dynamics of the taiga tick ixodes persulcatus in mixed coniferous and leaf-bearing forests of the udmurtia republic in the period 1957-1986 was carried out. a possibility to estimate the absolute number of all stages of i. persulcatus based on relative indices. females of i. persulcatus lay 20,250 eggs per 1 hectare, and this number of eggs gives birth to 15,000 larvae. from this number, 7870 larvae hibernate and 6550 individuals became fed. the number ... | 2004 | 15656092 |
| evaluation of groel gene analysis for identification of borrelia burgdorferi sensu lato. | the nucleotide sequences of the groel genes, the flagellin genes, and the 16s rrna genes from 22 reference strains of borrelia were compared. groel sequence analysis is useful not only in interspecies differentiation but also in intraspecies differentiation of borrelia afzelii and borrelia garinii isolates. | 2004 | 15004091 |
| [microorganism "montezuma" of the order rickettsiales: the potential causative agent of tick-borne disease in the far east of russia]. | a new microorganism, tentatively named "montezuma" was detected in ticks and in specimens (blood, bioptic specimens of the primary affect) taken from patients with an acute fever disease, etiologically linked with the bites of ixodes ticks in the far east of the russian federation. after sequencing the products of the amplification of dna isolated from ticks with wide-spectrum primers new primers were developed, highly specific to the unusual sequence thus obtained. the study revealed that ticks ... | 2004 | 15024973 |
| identification of three clinically relevant borrelia burgdorferi sensu lato genospecies by pcr-restriction fragment length polymorphism analysis of 16s-23s ribosomal dna spacer amplicons. | we report the results of a study of the prevalences of three clinically relevant borrelia burgdorferi sensu lato genospecies (borrelia burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii) in 1,040 questing ixodes ticks from all regions of latvia, where lyme borreliosis is endemic. the prevalences of borrelia in ixodes ricinus and ixodes persulcatus were 22.6 and 27.9%, respectively. molecular typing of b. burgdorferi from infected ticks was performed by restriction fragment length ... | 2004 | 15070987 |
| the emergence of lyme disease. | since its identification nearly 30 years ago, lyme disease has continued to spread, and there have been increasing numbers of cases in the northeastern and north central us. the lyme disease agent, borrelia burgdorferi, causes infection by migration through tissues, adhesion to host cells, and evasion of immune clearance. both innate and adaptive immune responses, especially macrophage- and antibody-mediated killing, are required for optimal control of the infection and spirochetal eradication. ... | 2004 | 15085185 |
| a novel alpha-proteobacterium resides in the mitochondria of ovarian cells of the tick ixodes ricinus. | an intracellular bacterium from ixodes ricinus ticks collected in italy was characterized by electron microscopy (em), pcr sequencing of the 16s rrna gene, molecular phylogenetic analysis, and in situ hybridization (ish). this bacterium was shown by em to be present in the cytoplasm, as well as in the mitochondria of ovarian cells. when universal 16s rrna bacterial primers were used, pcr amplification of ovarian dna followed by cloning and sequencing resulted in the same sequence being found in ... | 2004 | 15128508 |
| detection of a rickettsia closely related to rickettsia aeschlimannii, "rickettsia heilongjiangensis," rickettsia sp. strain rpa4, and ehrlichia muris in ticks collected in russia and kazakhstan. | using pcr, we screened 411 ticks from four genera collected in russia and kazakhstan for the presence of rickettsiae and ehrlichiae. in russia, we detected "rickettsia heilongjiangensis," rickettsia sp. strain rpa4, and ehrlichia muris. in kazakhstan, we detected rickettsia sp. strain rpa4 and a rickettsia closely related to rickettsia aeschlimannii. these agents should be considered in a differential diagnosis of tick-borne infections in these areas. | 2004 | 15131195 |
| survey of rodents and ticks in human babesiosis emergence area in japan: first detection of babesia microti-like parasites in ixodes ovatus. | babesia microti-like parasites were detected for the first time in ixodes ovatus in hyogo prefecture, japan, where two reported types of b. microti-like parasites were recognized in many rodents. of 80 adult i. ovatus ticks collected, 5 possessed the reported type and 1 possessed a new type of b. microti-like parasite. | 2004 | 15131209 |
| studies on the transovarial transmission of borrelia burgdorferi sensu lato in the taiga tick ixodes persulcatus. | the possibility of vertical transmission of borrelia burgdorferi sensu lato in ixodes persulcatus schulze, 1930 ticks was studied in the progeny of 20 females collected from the vegetation in an active focus of ixodid tick-borne borrelioses (itbb) located in the perm oblast, russia, where borrelia garinii and b. afzelii are circulating. the presence of borrelia dna was detected by the pcr method after feeding and egg laying in 16 engorged females (80.0%), as well as in 36.5 +/- 7.2% samples cont ... | 2004 | 15139380 |
| vectors of tick-borne diseases and epidemiological situation in latvia in 1993-2002. | during the period of 1993-2002 an increase and the remarkable changes in the incidence of tick-borne encephalitis (tbe) and lyme borreliosis (lb) as well as annual activities of vector species were noticed. the highest increase of tbe morbidity in latvia has been observed in 1994 and 1995, and less expressed also in 1998 which was followed by a significant decrease during subsequent years. whereas the highest peak of lb morbidity has been noticed in 1998 with only a minor decrease during subsequ ... | 2004 | 15146983 |
| first report on the coexistence and compatibility of seven tick-borne pathogens in unfed adult ixodes persulcatus schulze (acarina: ixodidae). | 2004 | 15146991 | |
| [long-term dynamics of the epizootic process in natural foci of ixodid tick borreliosis in mountain taiga forests of the middle ural]. | during eleven spring-summer seasons (1992-2002), the dynamics of the epizootic process in natural foci of ixodid tick-borne borrelioses (itbb) were studied in taiga forests of the middle ural (perm province, russia). in these foci, borrelia garinii and b. afzelii circulate, and their main vector is the ixodes persulcatus tick. main parameters of the epizootic process were calculated for each season. in four seasons (1993, 1996, 1999 and 2002), the parameters characterizing the abundance of unfed ... | 2004 | 15174388 |
| [new evidence for the detection of ehrlichia and anaplasma in ixodes ticks in russia and kazakhstan]. | polymerase chain reaction and sequence analysis were used to screen ehrlichia and anaplasma dna in 900 specimens of ixodes ticks of four genera (dermacetor, haemaphysalis, ixodes, and hyalomma) collected on 14 administrative territories of russia and kazakhstan. anaplasma phagocytophilia dna was detected and identified in ixodes persulcatus ticks gathered in the altai and primorye territories. ehrlichia muris dna was genotyped in the i. persulcatus ticks collected in the tyumen, omsk, and novosi ... | 2004 | 15193042 |
| [indication of borrelia burgdorferi sensu lato in the taiga tick larvae obtained from spontaneously infected females]. | hungry larvae from 17 clutches by spontaneously infected ixodes persulcatus females were examined by inoculation of the bsk ii medium, by microscopy of fixed smears, and by polymerase chain reaction (pcr) with the primers specific toward the conserved sequences of borrelia burgdorferi 16s ribosomal rna gene. a study of 781 larvae individually or as pools could not reveal borrelia dna by pcr. inoculations of the bsk ii medium with 600 larvae yielded no positive results either; immobile spiral for ... | 2003 | 14564837 |
| [long-term morbidity and spatial distribution of tick-borne encephalitis and ixodes tick-borne borreliosis in udmurtia]. | the paper considers the trends of morbidity due to tick-borne encephalities (tbe) and lxodes tick-borne borrelioses (ltbb) in udmurtia during 35 years (1965-2000) and evaluates the long-term influence of measures aimed at suppressing natural foci by eliminating the basic vector on this process. for this, data on 1062 cases of fevers occurring after the bite of a tick in udmurtia in 1965-1968, the similar data on 1509 cases in 1983-1987, and the official data on the incidence of these infections ... | 2003 | 14564842 |
| detection and typing of borrelia burgdorferi sensu lato genospecies in ixodes persulcatus ticks in west siberia, russia. | the prevalence of borrelia burgdorferi sensu lato (s.l.) genospecies in west siberia as well as in many other regions of russia remains insufficiently investigated. in the present study a total of 151 adult female ticks ixodes persulcatus schulze, collected at three localities in eastern regions of west siberia, where lyme disease is endemic, were examined for the presence of the spirochete b. burgdorferi s.l. by polymerase chain reaction targeting the 23s-5s rrna intergenic spacer regions. spir ... | 2003 | 14592703 |
| genetic diversity among borrelia strains determined by single-strand conformation polymorphism analysis of the ospc gene and its association with invasiveness. | lyme borreliosis (lb) is a tick-borne spirochetal infection caused by three borrelia species: borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto. lb evolves in two stages: a skin lesion called erythema migrans and later, different disseminated forms (articular, neurological, cardiac.). previous research based on analysis of ospc sequences allowed the definition of 58 groups (divergence of <2% within a group and >8% between groups). only 10 of these groups include all of the strains i ... | 2003 | 14605139 |
| detection of antibodies to anaplasma phagocytophilum and ehrlichia chaffeensis antigens in sera of korean patients by western immunoblotting and indirect immunofluorescence assays. | two hundred seventy one serum samples from south korean patients were tested to detect antibodies against anaplasma phagocytophilum (the human granulocytic ehrlichiosis agent) and ehrlichia chaffeensis (the human monocytic ehrlichiosis agent) by indirect fluorescent-antibody assay (ifa) and the western blot assay. these sera were collected from patients with symptoms of high fever. the rate of seropositivity for orientia tsutsugamushi was 50.9% by ifa at the public health & environmental researc ... | 2003 | 14607867 |
| evidence of babesia microti infection in multi-infected ixodes persulcatus ticks in russia. | to detect babesia-infected ixodes persulcatus shulze in a suburb of st. petersburg, russia, 738 adult ticks were studied using babesia specific primers and pcr techniques. the entire sample (more than 1,200 individuals) was screened for the presence of borrelia spp., ehrlichia spp. and tick-borne encephalitis virus (tbev). all 7 ticks infected with babesia microti, were also infected with other pathogens (all 7 among 417 infected ticks, zero amongst the remaining 321 naive ones (chi2 = 5.25, p<0 ... | 2003 | 14635819 |
| emerging infectious diseases in mongolia. | since 1990, mongolia's health system has been in transition. impressive gains have been accomplished through a national immunization program, which was instituted in 1991. nevertheless, the country continues to confront four major chronic infections: hepatitis b and c, brucellosis, tuberculosis, and sexually transmitted diseases (stds). as of 2001, only two cases of hiv infections had been detected in mongolia, but concern grows that the rate will increase along with the rising rates of stds and ... | 2003 | 14720388 |
| ability of ixodes persulcatus, haemaphysalis concinna and dermacentor silvarum ticks to acquire and transstadially transmit borrelia garinii. | in china, the ability of ixodes persulcatus, haemaphysalis concinna and dermacentor silvarum to transmit borrelia spirochetes was determined under laboratory conditions. results showed that all three tick species can acquire spirochetes by feeding on infected mice. however, the capability of the three species to maintain spirochetes was different. only i. persulcatus is able to maintain spirochetes alive during molting, and subsequent tick stages transmitted the infection to naive mice. in h. co ... | 2003 | 14756409 |
| characterization of borrelia burgdorferi sensu lato from novosibirsk region (west siberia, russia) based on direct pcr. | borrelia burgdorferi sensu lato infecting ixodes persulcatus ticks near novosibirsk, russia were detected using pcr with primers specific to 5s and 23s rrna genes. two genospecies, b. afzelii and b. garinii, were identified by the pcr-based restriction fragment length polymorphism analysis with tru9-i restriction endonuclease. comparison of the corresponding nucleotide sequences revealed considerable diversity of the 5s-23s intergenic spacer structure among b. garinii. | 2003 | 14758874 |
| [longevity of forest and taiga ticks (ixodidae) infected and non-infected with borrelia burgdorferi groups]. | a life longevity of ixodes ricinus and i. persulcatus have been examined in laboratory conditions. it was found that specimens infected with borreliae live slightly longer than non-infected ones (difference is not reliable); females live 1.5 times longer than males; i. ricinus lives 2-4 times longer than i. persulcatus. it is suggested that i. persulcatus has a higher rate of metabolism than i. ricinus and that the latter species obtains an ability to survive after the last moult two winters and ... | 2003 | 14964854 |
| survey of ixodid tick species on domestic cats in japan. | various species of ixodid ticks, attached to domestic cats in japan, were identified in spring (april to june) and autumn (september to november). in the spring, a total of 282 ticks, including 61 larvae, 70 nymphs, 127 females and 24 males were collected from 126 cats. of these, 264 were identified up to the species level. in the spring, haemaphysalis longicornis was the most frequently (39.7%, 50/126) found tick species on feline hosts, followed by ixodes ovatus (35.0%, 44/126), ixodes nippone ... | 2003 | 12531297 |
| [interrelations between ixodes persulcatus ticks and the tick-borne encephalitis virus of the red vole (clethrionomys rutilis) in western siberia]. | we present the data of 12-year survey (1989-2001) of the red vole population in southeastern west siberia, including estimation of host relative numbers, abundance of immature taiga ticks, and percentage of animals with antigemagglutitnins against tick-borne encephalitis (tbe) virus. we discuss the role of demographic groups of voles as tick's hosts and their participation in the maintenance of tbe causative agent population. the estimation of spontaneous tbe infection rate in summer as well as ... | 2003 | 12677668 |
| identification of ehrlichia chaffeensis, anaplasma phagocytophilum, and a. bovis in haemaphysalis longicornis and ixodes persulcatus ticks from korea. | a total of 1,467 tick (1,463 of haemaphysalis longicornis, three of ixodes persulcatus and one of i. turdus) collected from nine provinces of korea were examined by taqman real-time pcr for the presence of ehrlichia and anaplasma species. one set of primers and a probe were designed for detection of all of the ehrlichia and anaplasma species. template dnas (total 803) were prepared either from pools of larvae, nymphs, adult males and females, or from the salivary gland and midgut of adult ticks. ... | 2003 | 12804377 |
| prevalence of anaplasma phagocytophila and borrelia burgdorferi in ixodes persulcatus ticks from northeastern china. | a total of 1,345 ixodes persulcatus ticks collected from northeastern china were investigated for the presence of anaplasma phagocytophila and borrelia burgdorferi by a nested polymerase chain reaction (pcr). sixty-two (4.6%) ticks were positive for a. phagocytophila and 454 (33.8%) were positive for b. burgdorferi. seven (0.5%) were coinfected with both agents. sequence analysis of 919-basepair pcr amplicons revealed three types of a. phagocytophila. type 1 was identical to the published sequen ... | 2003 | 12812342 |
| "candidatus rickettsia tarasevichiae" in ixodes persulcatus ticks collected in russia. | we collected 209 ixodes persulcatus ticks in various regions of russia, including the southern urals and western and eastern siberia. using pcr amplification and sequencing of the citrate synthase-encoding gene (glta), we detected a new rickettsial genotype, which we named "candidatus rickettsia tarasevichiae." this bacterium was found in 9.27%, 10.0%, and 20.5% of the ticks collected in western siberia, eastern siberia, and the southern urals, respectively. "candidatus rickettsia tarasevichiae" ... | 2003 | 12860621 |
| [ixodes tick-borne borreliosis and tick-borne encephalitis in the udmurt republic: retrospective analysis of their spread]. | the primary clinical-anamnestic and laboratory data on 1,062 cases, registered in udmurtia in 1965-1968 and caused by the bites of taiga ticks (ixodes persulcatus), were retrospectively analyzed. the study revealed that not less than 27% of these cases could be regarded as cases of ixodes tick-borne borreliosis (itbb); formerly they were classified with tick-born encephalitis (tbe) or diseases of unclear etiology. the spread of itbb on the territory of the udmurt republic was shown to be similar ... | 2003 | 12886603 |
| [the possibility of the detection of one more tick-borne infection--babesiosis--on the territory of russia]. | a total of 739 taiga ticks of ixodes persulcatus species, obtained in the recreational zone of st. petersburg, were studied for the presence of babesia sp. with polymerase chain reaction. all these ticks underwent the preliminary examination for the presence of borrelia (3 species), ehrlichia (2 species) and tick-borne encephalitis (tbe) virus. in 7 cases babesia were detected among 413 ticks containing other pathogens. among 326 ticks no babesia were detected, as well as no other pathogens. all ... | 2003 | 12886630 |
| the taiga tick ixodes persulcatus (acari: ixodidae) in the sakha republic (yakutia) of russia: distributional and reproductive ranges. | the finding of an unfed adult female of the taiga tick ixodes persulcatus schulze is reported from the northern part of eastern siberia (the central part of the sakha republic [former yakutia]) of russia. this finding supplements other reported single findings of the taiga tick in different sites of the central part of the sakha republic, thus increasing its distributional range. the reproductive range of the taiga tick is limited to two separate areas in the southern parts of the republic. the ... | 2003 | 12597666 |
| real-time pcr for simultaneous detection and quantification of borrelia burgdorferi in field-collected ixodes scapularis ticks from the northeastern united states. | the density of spirochetes in field-collected or experimentally infected ticks is estimated mainly by assays based on microscopy. in this study, a real-time quantitative pcr (qpcr) protocol targeting the borrelia burgdorferi-specific reca gene was adapted for use with a lightcycler for rapid detection and quantification of the lyme disease spirochete, b. burgdorferi, in field-collected ixodes scapularis ticks. the sensitivity of qpcr for detection of b. burgdorferi dna in infected ticks was comp ... | 2003 | 12902243 |
| diagnosis of tick-borne encephalitis by a mu-capture immunoglobulin m-enzyme immunoassay based on secreted recombinant antigen produced in insect cells. | acute tick-borne encephalitis is diagnosed by detection of igm antibodies to tick-borne encephalitis virus (tbev) (genus flavivirus) in patient serum. tbev membrane (m) and envelope (e) proteins have previously been shown to form virus-like particles when expressed in mammalian cells. we expressed the prm/m and e proteins in insect cells with a recombinant baculovirus system and obtained antigenic protein secreted into the cell culture medium, as evidenced by detection by a panel of five monoclo ... | 2003 | 12958266 |
| [morphological barrier in mechanisms of reproductive isolation acting in areas of sympatry of closely related species ixodes persulcatus-i.pavlovskyi and i. persulcatus-i. ricinus (ixodidae)]. | a morphometric analysis of different male and female organs used in the process of copulation, including an attachment of the spermatophore to the genital opening of female and placing the spermatophore into the vestibular part of vagina, was carried out for the pairs of ixodid tick species ixodes persulcatus--i. pavlovskyi and i. persulcatus--i. ricinus in areas of their sympatry. in two disjunctive areas of sympatry of i. persulcatus--i. pavlovskyi (south-east altai and southern primorye), the ... | 2002 | 12624962 |
| [comparative characterization of a risk for infection with tick-borne encephalitis in the foci with different regional confinement]. | a comparative study of the foci of tick-borne encephalitis (tbe) has revealed that the infectivity of vectors taiga ticks (ixodes persulcatus) plays the leading role in the formation of foci with the highest epidemic activity (including forest steppe ones). the morbidity rates in each regional focus are directly related to the number of tick-bitten persons and to the quantity of the vector. | 2002 | 12557579 |
| [estimation of the absolute density of adult ixodes persulcatus ticks according to the results of collecting per flag-hour (ixodidae)]. | the results of two methods of estimation of abundance of adult ixodes persulcatus ticks, the absolute (at the sample plots) and the relative (per flag-hour) estimations, were compared. collecting of ticks and estimation of their abundance was conducted during 9 years at the forests of the far east and pre-ural region. the total of 1995 plots (100 sq. m each) were studied and 865 flag-hours were carried out. a good correlation of the data, obtained by these methods, was revealed. the average numb ... | 2002 | 11962198 |
| genetic identification of rickettsiae isolated from ticks in japan. | following the description in japan of japanese spotted fever, caused by rickettsia japonica, a search for the vector of this disease led to the isolation of several rickettsiae from various tick species. sixty-three rickettsial isolates were obtained from six different tick species, and six type strains were described by pcr and monoclonal antibody testing. we identified these six strains by amplification and sequencing of the genes encoding 16s rrna and citrate synthase. we confirmed that the i ... | 2002 | 12037083 |
| borrelia burgdorferi population kinetics and selected gene expression at the host-vector interface. | by using real-time quantitative pcr, the population dynamics and gene transcription of borrelia burgdorferi were examined in ticks and skin of mice during acquisition of the infection from mice by ticks and during transmission of the infection from ticks to mice. population dynamics were determined by using a flab dna target. a quantitative analysis of flab, ospa, ospc, dbpa, and arp transcription was also performed. the results revealed that both uninfected larval and nymphal ixodes scapularis ... | 2002 | 12065476 |
| simultaneous detection of anaplasma marginale and a new ehrlichia species closely related to ehrlichia chaffeensis by sequence analyses of 16s ribosomal dna in boophilus microplus ticks from tibet. | to identify ehrlichial agents in boophilus microplus ticks, dna samples of b. microplus collected from the tibet autonomous region and sichuan province of china were screened by a nested pcr. sixteen of 43 (37%) dna samples of b. microplus from tibet were positive in nested pcr analysis. all 27 samples from sichuan were negative. the screen identified two ehrlichial agents based on different 16s rrna genes that were found after amplifying and sequencing the 5'-end fragments of the 16s rrna genes ... | 2002 | 12202567 |
| determination of novel borrelia genospecies in swedish ixodes ricinus ticks. | a total of 301 adult questing ixodes ricinus ticks were collected at 15 different locations along the south and east coasts of sweden to determine the borrelia genospecies diversity. thirty-two ticks (11%) were found to be positive by nested pcr with borrelia burgdorferi sensu lato-specific primers. species determination was based on partial sequencing of the 16s rrna gene and the flagellin gene. five different borrelia species were found. the nucleotide sequence of the borrelia dna found in two ... | 2002 | 12202571 |
| [risk of human infection with ixodes tick-borne relapsing fever in different regional subzones of the perm region]. | serological and epidemiological surveys covered 2407 persons in 20 populated localities situated in all large geographical and regional subdivisions (subzones) in the perm region. indirect immunofluorescence tests revealed serum immunoglobulins to borrelia burgdorferi sensu lato. antibodies in 1:40 or higher dilution were found in nearly 5 to 11% of the dwellers of localities in the subzones of south taiga, coniferous broad-leaved forests, and forest steppe; about 3-7% and 1-6% of them were pres ... | 2002 | 12224266 |
| [a laboratory model of the parasitic system of lyme disease]. | a laboratory model of the parasitic system of lyme borreliosis (lb) with ixodes persulcatus and borrelia burgdorferi s.s. underwent 5 epizootic cycles (cycle i transition of borreliae by the scheme a tick-->a mouse-->a tick corresponds to the seasonal cycle). a group of 5-6 mice was inoculated with the suspension of infected nymphs by the intracutaneously route and 35-45 days later intact tick larvae or nymphs were fed on them. in the first three cycles, 40-100% of mice in a group (a total of 33 ... | 2002 | 12224267 |
| pcr detection of borrelia burgdorferi sensu lato, tick-borne encephalitis virus, and the human granulocytic ehrlichiosis agent in ixodes persulcatus ticks from western siberia, russia. | pcr assays were used to test adult ixodes persulcatus ticks from western siberia, russia, for borrelia burgdorferi sensu lato, tick-borne encephalitis virus (tbev), and the human granulocytic ehrlichiosis (hge) agent. of the 150 ticks that were studied, 38% were infected with b. burgdorferi, 46% were infected with tbev, and 8% were infected with the hge agent. these three pathogens were distributed in the ticks independently of one another. | 2002 | 12354885 |
| [detection and sequential analysis of granulocytic ehrlichia 444-epank gene]. | to provide further pathogenic evidence of granulocytic ehrlichia infection in china. | 2002 | 12411075 |
| [exploratory study on natural focus and its causative agent of genotype of lyme disease by polymerase chain reaction in the forest areas of beijing]. | to learn the existence of natural focus of lyme disease and its distribution. | 2002 | 12411092 |
| seasonal correlation of sporadic schizophrenia to ixodes ticks and lyme borreliosis. | background: being born in winter and spring is considered one of the most robust epidemiological risk factors for schizophrenia. the aetiology and exact timing of this birth excess, however, has remained elusive so far. since during phylogeny, borrelia dna has led to multiple germ-line mutations within the cb1 candidate gene for schizophrenia, a meta analysis has been performed of all papers on schizophrenic birth excesses with no less than 3000 cases each. all published numerical data were then ... | 2002 | 12453316 |
| [reason for fluctuation in the demographic structure of the taiga tick population (ixodidae) in dark conifer-leaf forests of kemchug upland]. | it was found out, that mean ratio of unfed taiga ticks ixodes persulcatus having a life cycle of 3-5 and 6 years counts respectively (n = 6) 33.6, 52.8, 13.2 and 0.4%. data on absolute number of i. persulcatus individuals in the process of onthogenesis is given. it is shown, that mortality of different unfed stages increases from larva to imago. in the autumn-winter period, the mean ratio of eliminated individuals counted 16% of larvae, 20% of nymphs, and 38 of imago. the mortality in the spring ... | 2002 | 12481603 |
| photoperiodic control of developmental diapause in nymphs of prostriate ixodid ticks (acari: ixodidae). | extrinsic control of developmental diapause in nymphs of prostriate ticks of the subgenus ixodes sensu stricto (ixodes ricinus and ixodes persulcatus from eurasia and ixodes scapularis from north america) appears to be based on a complex two-step photoperiodic reaction of a short-day/long-day type. diapause control in the subgenus afrixodes (the south african tick ixodes rubicundus) appears to be based on a simple long-day reaction. the option between non-diapause development and diapausing arre ... | 2002 | 14570127 |
| tick-host-borrelia population interactions: long-term records in eastern europe. | during 10 spring and summer seasons (1992-2001), the dynamics of the epizootic process in natural foci were studied in taiga forests of the pre-ural region, russia. in these foci, borrelia garinii, borrelia afzelii and tick-borne encephalitis (tbe) virus circulate transmitted by their main vector tick, ixodes persulcatus. main parameters of the epizootic process were calculated for each season. in three seasons (1993, 1996, and 1999) all parameters characterizing the abundance of unfed nymphs an ... | 2002 | 14570135 |
| the occurrence of parasitic arthropods in two groups of european bison in the białowieza primeval forest. | within 1992-2000, a total of 181 białowieza forest bison were examined from two winter herds. twelf parasitic arthropod species were observed, a high infestation being typical of demodex bisonianius, chorioptes bovis, ixodes ricinus, dermacentor reticulatus, and bisonicola sedecimdecembrii. the infestation in section 422 herd being higher for b. sedecimdecembrii, i. ricinus, d. reticulatus, ch. bovis. d. bisonianus was slightly more prevalent in the section 391 herd, the intensity being, however ... | 2001 | 16886430 |
| tick-borne encephalitis virus in lithuania. | tick-borne encephalitis (tbe) is a severe problem in lithuania, indicated by the 171 to 645 serologically confirmed cases that occurred each year between 1993 and 1999. in the present report, the first isolation and partial genetic analysis of a lithuanian tbe virus (tbev) strain isolated from a patient's serum sample is described. the patient was bitten by a tick while visiting the lazdijai district (veisiejai forest) in the southernmost part of lithuania, a geographical area where ixodes ricin ... | 2001 | 11837641 |
| [multistage mechanism of reproductive sympatric isolation of the closely-related species, ixodes persulcatus and i. pavloskyi (ixodidae)]. | the closely related species ixodes persulcatus and i. pavlovskyi are vectors of agents tick-born encephalitis and lyme disease. these species have two great disjunctive sympatric distribution areas (fig. 1), where both species inhabit the same biotopes, and during the same season. in these conditions there are prerequisites of contacts of opposite sexes of the different species during ambushing the hosts on vegetation. sequence of "switching on" of the morphological, physiological, and hostparas ... | 2001 | 11871251 |
| [involvement of the common shrew, sorex araneus (insectivora, soricidae), in circulation of the tick-borne encephalitis virus in south-western siberia]. | we presented the data on the abundance of immature instars of the taiga tick ixodes persulcatus schuize on the common shrews sorex araneus l. in natural foci of tick-borne encephalitis in the south of western siberia. basing on the results of virological and serological studies we demonstrated a low effectiveness of this host species as a donor of disease agent strains, which are predominant in the territory under study, for ticks feeding on shrews. the analysis of samples taken from the young s ... | 2001 | 11871252 |
| [histopathologic changes of bird skin in feeding places of ticks of the genus ixodes (acari: ixodidae)]. | histopathological changes of skin of passeriformes birds in the places of attachment and feeding of the ixodes persulcatus and i. lividus were investigated. the inflammation of bird skin is analogous to one of mammalian skin. the mouthparts of tick are surrounded by the fibrin cone and collagen capsula. they are formed by host. ticks of these species do not form the cement. a proliferation stage of skin inflammation in birds is expressed. the thick collagen capsula develops in a result of prolif ... | 2001 | 11881134 |
| lone star tick-infecting borreliae are most closely related to the agent of bovine borreliosis. | although borrelia theileri, the agent of bovine borreliosis, was described at the turn of the century (in 1903), its relationship with borreliae causing lyme disease or relapsing fever remains undescribed. we tested the previously published hypothesis that spirochetes infecting lone star ticks (amblyomma americanum) may comprise b. theileri by analyzing the 16s ribosomal dnas (rdnas) and flagellin genes of these spirochetes. b. theileri, the amblyomma agent, and b. miyamotoi formed a natural gro ... | 2001 | 11158095 |
| a relapsing fever group spirochete transmitted by ixodes scapularis ticks. | a species of borrelia spirochetes previously unknown from north america has been found to be transmitted by ixodes scapularis ticks. infected ticks are positive for borrelia spp. by dfa test but negative for borrelia burgdorferi by polymerase chain reaction (pcr) using species-specific primers for 16s rdna, outer surface protein a, outer surface protein c, and flagellin genes. a 1,347-bp portion of 16s rdna was amplified from a pool of infected nymphs, sequenced, and compared with the homologous ... | 2001 | 12653133 |
| specificities and sensitivities of four monoclonal antibodies for typing of borrelia burgdorferi sensu lato isolates. | borrelia burgdorferi, the agent of lyme borreliosis, is genetically more heterogeneous than previously thought. in europe five genospecies have been described from the original b. burgdorferi sensu lato (sl): b. burgdorferi sensu stricto (ss), b. garinii, b. afzelii, b. lusitaniae, and b. valaisiana. in the united states, b. burgdorferi ss as well as b. bissettii in california and b. andersonii on the east coast were differentiated. in asia, b. japonica has been identified along, with b. garinii ... | 2001 | 11238225 |
| the prevalence of borrelia burgdorferi sensu lato in ixodes persulcatus and i. ricinus ticks in the zone of their sympatry. | a total of 7210 unfed adult ixodes persulcatus schulze, 1930 and i. ricinus (l., 1758) ticks were collected from the vegetation by flagging in 35 study sites located in the zone of their sympatry (mainly in leningrad region, russia). borrelia infection in ticks was estimated by the dark-field microscopic analysis of gut contents in standard vital preparations at a magnification of x600. no correlation was revealed between the series of parameters characterising the abundance of each tick species ... | 2001 | 11266136 |
| characterization of lyme borreliosis isolates from patients with erythema migrans and neuroborreliosis in southern sweden. | southern sweden is an area of lyme borreliosis (lb) endemicity, with an incidence of 69 cases per 100,000 inhabitants. the most frequent clinical manifestations are erythema migrans (77%) and neuroborreliosis (16%). there was no record of human borrelia strains being isolated from patients in this region before the prospective study reported here. borrelia spirochetes were isolated from skin and cerebrospinal fluid (csf) from lb patients living in the region. a total of 39 strains were character ... | 2001 | 11283044 |
| p13, an integral membrane protein of borrelia burgdorferi, is c-terminally processed and contains surface-exposed domains. | to elucidate antigens present on the bacterial surface of borrelia burgdorferi sensu lato that may be involved in pathogenesis, we characterized a protein, p13, with an apparent molecular mass of 13 kda. the protein was immunogenic and was expressed in large amounts during in vitro cultivation compared to other known antigens. an immunofluorescence assay, immunoelectron microscopy, and protease sensitivity assays indicated that p13 is surface exposed. the deduced sequence of the p13 peptide reve ... | 2001 | 11292755 |
| distribution and characterization of tick-borne encephalitis viruses from siberia and far-eastern asia. | in this study, tick-borne encephalitis (tbe) viruses from siberia and far-eastern asia were characterized in order to determine virus subtype distribution. tbe viruses were isolated from ticks (ixodes persulcatus) collected in the far-eastern (khabarovsk and vladivostok) and siberian (irkutsk) regions of russia in 1999. phylogenetic analysis showed that isolates formed distinct clusters of far-eastern and siberian subtypes. there was also a minor difference in antigenicity between the irkutsk is ... | 2001 | 11369875 |
| human babesiosis in japan: isolation of babesia microti-like parasites from an asymptomatic transfusion donor and from a rodent from an area where babesiosis is endemic. | to determine the source of infection for the japanese index case of human babesiosis, we analyzed blood samples from an asymptomatic individual whose blood had been transfused into the patient. in addition, we surveyed rodents collected from near the donor's residence. examination by microscopy and pcr failed to detect the parasite in the donor's blood obtained 8 months after the donation of the blood that was transfused. however, we were able to isolate babesia parasites by inoculating the bloo ... | 2001 | 11376054 |