Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
treatment of extraoral sinus tracts from traumatized teeth with apical periodontitis.when a draining lesion is encountered on the skin of the face, an endodontic origin should always be considered in differential diagnosis. non-surgical endodontic therapy, sometimes complimented by surgery, or extraction are the choices for the treatment of these cases. three cases of extraoral sinus tract on the chin caused by necrotic pulp of traumatized lower anterior teeth are presented. a paste consisting of calcium hydroxide and barium sulfate powder mixed with glycerin was used. usage of ...19957641626
characterization of inhibitory effects of suspected periodontopathogens on osteogenesis in vitro.by using an in vitro bone-forming culture system, the chick periosteal osteogenesis (cpo) model, the direct effects on osteogenesis of sonicated extracts derived from oral bacteria were examined. both extracts from bacterial species having strong associations with periodontal diseases (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and prevotella intermedia, hereinafter referred to as suspected periodontopathogens) and extracts from species not correlated with periodontal diseas ...19957642257
detection of tet(m) and tet(o) using the polymerase chain reaction in bacteria isolated from patients with periodontal disease.the polymerase chain reaction was used to examine 114 tetracycline-resistant anaerobic and facultative anaerobic bacterial isolates from patients with periodontal disease for the tet(m) and tet(o) genes. a 740-base-pair fragment of the tet(m) gene was amplified from 84 of 114 isolates, and a 519-base-pair fragment of the tet(o) gene was amplified from 13 streptococcal isolates. six of 7 tetracycline-resistant isolates of veillonella spp. and tetracycline-resistant isolates of eubacterium spp. (n ...19957675524
synergism of lactate and succinate as metabolites utilized by veillonella to inhibit the growth of salmonella typhimurium and salmonella enteritidis in vitro.the inhibition of salmonellae growth by a veillonella bacterium isolated from the cecal contents of adult chickens was examined. the veillonella isolate was grown on an agar medium supplemented with 175 mumol of lactate or succinate/ml. either 0, 100, 125, 150, or 175 mumol of succinate/ml was added to the lactate medium; either 0, 100, 125, 150, or 175 mumol of lactate/ml was added to the succinate medium; and the ph of all media was adjusted to 6.0. agar overlays of veillonella cultures grown ...19957677651
expression of the sodium ion pump methylmalonyl-coenzyme a-decarboxylase from veillonella parvula and of mutated enzyme specimens in escherichia coli.the structural genes of the sodium ion pump methylmalonyl-coenzyme a (coa)-decarboxylase from veillonella parvula have recently been cloned on three overlapping plasmids (pjh1, pjh20, and pjh40) and sequenced. to synthesize the complete decarboxylase in escherichia coli, the genes were fused in the correct order (mmdadecb) on a single plasmid (pjh70). a dna region upstream of mmda apparently served as promoter in e. coli because expression of the mmd genes was not dependent on the correct orient ...19957601825
a comparison of the microbial flora in carious dentine of clinically detectable and undetectable occlusal lesions.it is not known whether the aetiology of occlusal hidden caries lesions (hcl) is identical to that of small visible lesions (svl). previous studies of the microflora of hcl suggest that relatively few species can be isolated. the aim of the present study was to compare the bacterial composition of dentine from 10 hcl and 17 svl in a population of children aged 8-18 years. the following bacteria were identified: actinomyces spp., mutans streptococci, streptococcus sanguis, streptococcus oralis, s ...19957867050
head and neck space infections in infants and children.a retrospective study was performed in 117 children with head and neck space infections treated at the children's hospital of pittsburgh from january 1986 through june 1992. peritonsillar space infections were the most common (49%), followed by retropharyngeal (22%), submandibular (14%), buccal (11%), parapharyngeal (2%), and canine (2%) space infections. the most common pathogens isolated (n = 78) were the aerobes beta-hemolytic streptococcus (18%) and staphylococcus aureus (18%), the anaerobes ...19957870436
microbiological assessment of an improved stannous fluoride dentifrice.recently, a new stabilized stannous fluoride (snf2) dentifrice (ssf) has been developed. the aim of the present work was to examine the antimicrobial activity of snf2, and to assess the long-term microbial safety of this dentifrice in a series of in vitro and clinical evaluations. results of in vitro time-kill experiments with representative oral bacteria demonstrated that snf2 exerts broad antimicrobial activity against both gram-positives and gram-negatives and, in particular, has potent activ ...19958593200
microbial alterations in supragingival dental plaque in response to a triclosan-containing dentifrice.a total of 325 subjects were entered into a double-blind, stratified 2-treatment clinical study that examined the effects of a dentifrice containing 0.3% triclosan, 2% gantrez copolymer and 0.243% sodium fluoride on supragingival dental plaque and gingivitis. a subset of 159 subjects including 72 men and 87 women participated in the microbiological component of this study, which was designed to detect shifts in supragingival bacterial species in response to triclosan. subjects were divided into ...19958602338
comparison of diagnostic tests for bacterial kidney disease in juvenile steelhead trout (oncorhynchus mykiss).in order to accurately diagnose bacterial kidney disease caused by renibacterium salmoninarum in steelhead trout, kidney tissue from experimentally infected fish was evaluated using a commercially available enzyme-linked immunosorbent assay (elisa) test kit, fluorescent antibody (fa) testing, bacteriologic culture, and histopathology. seventy-five steelhead trout were randomly assigned to 1 of 4 groups and intraperitoneally inoculated with 0.15 ml saline (n = 20), 1 x 10(10) organisms/ml (n = 18 ...19958580171
antibacterial activity of the metabolic by-products of a veillonella species and bacteroides fragilis.a veillonella species and bacteroides fragilis were isolated from the cecal contents of adult chickens. when growth on an agar medium supplemented with 0.4% glucose and adjusted to ph 6.5, mixed cultures containing veillonella and b. fragilis inhibited the growth of salmonella typhimurium; salmonella enteritidis, escherichia coli 0157:h7 and pseudomonas aeruginosa. decreasing the glucose concentration of the agar decreased the inhibitory activity of the mixed culture. mixed cultures grown on aga ...199516887516
resistance of veillonella to hydrosoluble pristinamycins: a possible outer membrane barrier effect.an in vitro study on 25 veillonella strains showed a consistent clindamycin susceptibility with resistance to erythromycin and to the hydrosoluble pristinamycins, quinupristin and dalfopristin and its 30:70 combination, the synergistin rp59500. double erythromycin-clindamycin disk tests did not show any inducible resistance pattern. the addition of 10 or 50 mug/ml of polymyxin b nonapeptide, an outer membrane permeabilizing agent, consistently reduced quinupristin and dalfopristin mics in most s ...199516887512
a comparative study of glucose and galactose uptake in pure cultures of human oral bacteria, salivary sediment and dental plaque.the ability to utilize glucose and the weaker sugar acidogen, galactose, was surveyed in salivary sediment, pooled dental plaque, and in pure cultures of the bacteria that numerically comprise most of the bacteria in these mixed microbial systems. except for a veillonella isolate, which showed no uptake of either sugar, glucose was utilized more rapidly than galactose by the 27 pure cultures tested and by both sediment and plaque. this sugar difference was also seen for two other measures of gly ...19957487576
phylogenetic placement of dialister pneumosintes (formerly bacteroides pneumosintes) within the sporomusa subbranch of the clostridium subphylum of the gram-positive bacteria.the nucleotide sequence of the 16s rrna gene of the type strain of dialister pneumosintes was determined. phylogenetic analysis revealed that this species belongs to the sporomusa branch of the clostridium subphylum of the gram-positive bacteria and should therefore be excluded from the family bacteroidaceae. within this branch, which encompasses several other gram-negative taxa, such as acidaminococcus, pectinatus, phascolarcobacterium, quinella, selenomonas, and zymophilus, dialister showed a ...19957537075
predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis.the purpose of this investigation was to study the microflora of severe, moderate and minimal periodontal lesions, in young adults with rapidly progressive periodontitis (rpp). subgingival plaque samples were taken from 142 periodontal lesions in 10 young adults aging 25 to 35 years. the examination of the subgingival microflora indicated that certain species, including porphyromonas gingivalis, bacteroides forsythus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, and campylobact ...19957722848
comparison of periodontal disease in hiv seropositive subjects and controls (ii). microbiology, immunology and predictors of disease progression.the aims of this study were to compare the prevalence of suspected periodontal pathogens in subgingival plaque from 29 hiv seropositive and 27 control subjects and to determine the association of these bacteria with periodontal destruction. subgingival plaque was collected from the mesiobuccal sites of all teeth, except 3rd molars. bacteria were identified and enumerated using non-isotopic whole chromosomal dna probes and a colony lift method. at baseline, hiv seropositive subjects had significa ...19957560241
effect of proteolytic enzymes on the lysis and growth of oral bacteria.previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ...19947478762
increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes.a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ...19947851086
[therapeutic possibilities in recurrent infantile tonsillitis].the present study compares two therapy protocols in 40 children with recurrent tonsillitis. twenty of them were randomly treated with penicillin, and the remaining 20 received penicillin + metronidazole. clinical and microbiological assessment of the results was made. the association of penicillin and metronidazole was most efficient, probably due to its activity against streptococus pyogenes and anaerobic betalactamase-producing bacterias. the high failure rate in the penicillin group states th ...19947873233
single polymerase chain reaction for the detection of tetracycline-resistant determinants tet k and tet l.oligonucleotide primers were used in polymerase chain reaction assays to detect tetracycline-resistant determinants tet k and tet l. forty-three isolates representing 11 genera carrying tet k and/or tet l determinants gave appropriate pcr products. the pcr products hybridized with labelled tet k or tet l probes, and differentiated the tet k from the tet l pcr products. we confirmed that two haemophilus aphrophilus carried the tet k determinant and two veillonella parvula carried the tet l determ ...19947877638
anaerobic susceptibility testing. slight differences in inoculum size can make a difference in minimum inhibitory concentrations.in the present study, we compared the actual inoculum density from inoculated broth microdilution wells to the targeted inoculum size (10(5) cfu/well) when the inoculum was prepared using a mcfarland nephelometer to achieve the standard density. three target inoculum sizes (10(5), 5 x 10(5), and 10(6) cfu/well) were used to compare the effect of slight inoculum size increases of both atcc and clinical strains of anaerobes on mics of various antimicrobials. actual colony counts of bacteroides fra ...19947924212
increased activity of a new chlorofluoroquinolone, bay y 3118, compared with activities of ciprofloxacin, sparfloxacin, and other antimicrobial agents against anaerobic bacteria.a total of 435 clinical isolates of anaerobes were tested with a broth microdilution method to determine the activity of bay y 3118 compared with those of other agents against anaerobic bacteria. all strains of bacteroides capillosus, prevotella spp., porphyromonas spp., fusobacterium spp., clostridium spp., eubacterium spp., peptostreptococcus spp., and veillonella parvula were susceptible (mics of < or = 2 micrograms/ml) to bay y 3118. against the 315 strains of the bacteroides fragilis group, ...19947979307
the predominant cultivable flora of sound and carious human root surfaces.little detailed knowledge exists about the composition of the human root flora. therefore, the predominant cultivable flora (pcf) was determined for samples of: (1) plaque from sound root surfaces (srs) in eight subjects without root caries, (2) plaque from incipient root lesions (irl) in eight subjects with root caries, and (3) carious material from advanced root lesions (arl) in nine other subjects with root caries. generally, one root surface was sampled per subject, and organisms-358, 512, a ...19947983259
relationship of oral microflora with oral health status in parkinson's disease.parkinson's disease (pd) patients report an increased craving for sweets, which may have an effect on microflora. we compared patients of pd who crave sweets with pd patients who do not. age- and sex-matched control subjects were used, with 14 subjects in each group. a plaque sample was taken from tooth #18 with a curette and placed into rtf, homogenized, and plated onto selective and non-selective media. microflora were expressed as % cfu's of total anaerobes. statistical analysis was performed ...19947716701
impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis.the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ...19948195981
the oral gram-negative anaerobic microflora in young children: longitudinal changes from edentulous to dentate mouth.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. the bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). during the primary period, 2 samples were collected from each child: a sample with d ...19947936718
in vitro activity of meropenem compared with imipenem, metronidazole, ampicillin, and ampicillin/sulbactam against anaerobes.the aim of the present study was to compare the in vitro activity of meropenem (ici 194660, cas 96036-03-2) with imipenem, metronidazole, clindamycin, ampicillin and ampicillin/sulbactam against a variety of anaerobic bacteria using an agar dilution method. 423 clinical isolates were tested belonging to 70 species of 15 anaerobic genera. they included bacteroides fragilis (n = 62), bacteroides thetaiotaomicron (n = 45), prevotella bivia (n = 11), fusobacterium nucleatum (n = 12), clostridium per ...19947945523
evaluation of the antimicrobial effectiveness of citric acid and sodium hypochlorite on the anaerobic flora of the infected root canal.this study was designed to evaluate the antimicrobial effectiveness of 25% citric acid, compared with 2.5% sodium hypochlorite, after time intervals of 5, 15, 30 and 60 min. anaerobic bacterial species were isolated from infected root canals and categorized according to gram stain as gram-positive cocci, gram-negative cocci, gram-positive rods and gram-negative rods. the experimental method used was similar to that proposed by the german society for hygiene and microbiology (dghm) with slight mo ...19947995646
chemostat flow cell system: an in vitro model for the evaluation of antiplaque agents.we developed an experimental in vitro model of dental plaque to assess the potential efficacy of antiplaque agents. the model used a chemostat, which provided a continuous source of 5 species of oral bacteria grown in an artificial "saliva-like" medium. this mixture was pumped through six flow cells, each containing two types of surfaces on which plaque formed and was subsequently measured. formation of bacterial plaque on hydroxyapatite surfaces was assessed by measurement of the dna and protei ...19947983262
the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration.obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ...19948004324
the effects of a 0.3% triclosan-containing dentifrice on the microbial composition of supragingival plaque.144 subjects completed a 6-month, double-blind study which examined the effects of a 0.3% triclosan/2% copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. the subjects were randomly assigned to use, in an oral hygiene program, either the triclosan/copolymer/fluoride test dentifrice or a control dentifrice. the latter had the same formulation as the test dentifrice except it did not contain triclosan. supragingival plaque was collected from the buccal and ...19948034778
anaerobic flora of the conjunctival sac in patients with aids and with anophthalmia compared with normal eyes.relatively few investigations of anaerobic bacteria as ocular flora have been conducted, and their results have been contradictory. the conjunctival sacs of 22 normal subjects and of 14 patients with acquired immunodeficiency syndrome, and 22 anophthalmic sockets were cultured for anaerobic bacteria thirty-four (77.3%) of the 44 eyes of normal subjects harbored anaerobic bacteria; propionibacterium acnes was present in 28 eyes (63.6%), lactobacillus species in 6 eyes (13.6%), and veillonella spe ...19948079632
bacterial colonization and degradation of demineralized dentin matrix in situ.lesion formation in dentin involves demineralization followed by degradation of the exposed organic matrix. proteinases from microorganisms present in the dentin are believed to play an important role in the breakdown of the dentinal collagen. in this study, the microflora colonizing decalcified dentin matrix, placed in situ, was identified. the gelatinolytic activity of the isolated strains was assessed and related to the degradation of the dentin matrix. the predominant species found were stre ...19948124693
[antimicrobial activities of cefteram against recently clinically detected and isolated strains from patients with dental infections].to investigate the antibiotic activity of cefteram (cftm), the minimum inhibitory concentrations (mics) of cftm and of the control drugs were determined against clinically isolated strains received from november 1991 to april 1993 from 19 dental facilities throughout the country, as well as against clinically isolated strains from samples obtained at this center from patients with dental infectious diseases, and the following results were obtained. 1. 430 strains were detected in 198 cases but i ...19948151907
fortuitous detection of prosthetic valve endocarditis caused by an uncommon etiologic agent. 19948159046
the effects of triclosan and zinc citrate, alone and in combination, on a community of oral bacteria grown in vitro.a mixed-culture chemostat system has been used as a more stringent laboratory system for evaluation of the antimicrobial effects of triclosan and zinc citrate. the inhibitors were added alone, and in combination, as a pulse (a high initial inhibitor concentration which decreased with time) or as a dose (concentration of inhibitor increased with time) to give maximum concentrations of 34.5 mumol/l triclosan and 39.8 mumol/l zinc citrate. when dosed, triclosan inhibited a. viscosus and all five gr ...19938380287
cytotoxicity versus antibacterial activity of some antiseptics in vitro.through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. a follow-up study was conducted to examine the germicidal effect of 0.05% naocl, 10 mg/ml metronidazole and supernatant of ca (oh)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. in vitro testi ...19938100851
tetracycline resistance in periodontal pathogens.antimicrobial agents are used in combination with debridement to eliminate putative periodontal pathogens, including porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans, from diseased tissues. the most frequently used antimicrobial agents are the tetracyclines. however, these agents are not effective in some patients. this lack of efficacy may be due to antimicrobial resistance. as many as 75% of the bacteria in the subgingival flora may be resistant to tetr ...19938324137
antibacterial effects of various endodontic irrigants on selected anaerobic bacteria.this study was undertaken to determine the antibacterial effects of various endodontic irrigants against six selected anaerobic bacteria. the organisms were mixed separately with dilutions of each of the irrigants in tubes containing fluid thioglycollate broth and allowed to remain in contact for specific time periods before being evaluated for growth. of the six irrigants tested, chlorhexidine appeared to be the most effective antibacterial substance. hydrogen peroxide, sodium hypochlorite and ...19938243347
microbial colonization of human tooth surfaces. 19938494649
a comparative study of cephradine, amoxycillin and phenoxymethylpenicillin in the treatment of acute dentoalveolar infection.a comparative double blind trial was undertaken of the efficacy of cephradine, amoxycillin and phenoxymethylpenicillin in the treatment of acute dentoalveolar abscesses. patients were admitted to the trial with acute dentoalveolar abscesses with systemic involvement and assessments were made of pain, swelling, temperature and lymphadenopathy. after admission to the trial appropriate surgical therapy was undertaken. the patients were then given either phenoxymethylpenicillin 250 mg four times dai ...19938494665
antibacterial activity of oral antibiotics against anaerobic bacteria.the spectrum and the antibacterial susceptibility of anaerobic bacteria to oral antibiotics isolated from clinical specimens was assessed in two different centres, the first receiving specimens from university departments and the second from general practitioners and small hospitals. susceptibility was studied with a microtiter ready-to-use panel system, using the manufacturer's modified wilkins-chalgren's broth as the test medium for the following antibiotics: ampicillin, ampicillin+sulbactam, ...19938508686
investigation of the influences of puberty, genetics, and environment on the composition of subgingival periodontal floras.the classical twin model was utilized in this study in an attempt to determine the importance of host genetics to the composition of the subgingival flora. simultaneously, the effect of puberty on the flora composition was assessed. the compositions of the floras were significantly different at ages 11 and 14 in the same people, indicating that transition to an adult flora composition may be initiated during puberty. however, the numbers of subjects who had prepubertal and postpubertal testoster ...19938514392
the effect of tetronasin and monensin on fermentation, microbial numbers and the development of ionophore-resistant bacteria in the rumen.the gram-negative rumen bacteria fibrobacter succinogenes s85, prevotella ruminicola m384 and veillonella parvula l59 were grown in media containing successively increasing concentrations of the ionophores, monensin and tetronasin. all three species became more resistant to the ionophore with which they were grown. increased resistance to one ionophore caused increased resistance to the other, and cross-resistance to another ionophore--lasalocid--and an antibiotic--avoparcin. recovery of tetrona ...19938407673
dental plaque development on defined streptococcal surfaces.coaggregations between bacterial species have been widely studied in vitro but not in the mouth. a new in vivo assay was used to measure the rate and composition of indigenous plaque formation onto bovine enamel chips covered with a continuous layer of bacteria. chips were covered with streptococcus oralis atcc 10557, which coaggregated with many oral species, or streptococcus gordonii s7, which did not coaggregate with these oral species, and placed in the mouth for 4 and 24 h. there were no di ...19938510979
sequence of the sodium ion pump methylmalonyl-coa decarboxylase from veillonella parvula.the genes encoding methylmalonyl-coa decarboxylase from veillonella parvula were cloned on plasmids using oligonucleotides derived from n-terminal amino acid sequences as specific probes. the entire dna sequence of the methylmalonyl-coa decarboxylase genes together with upstream and downstream regions was determined. the genes encoding subunits alpha (mmda), delta (mmdd), epsilon (mmde), gamma (mmdc), and beta (mmdb) of the decarboxylase were clustered on the chromosome in the given order. the p ...19938227015
the effect of glass-ionomer cement on carious dentine: an in vivo study.the effect of a glass-ionomer cement sealant on carious dentine was investigated clinically and bacteriologically. the study group consisted of 24 molar teeth, with small clinical visible occlusal dentine lesions, in 13 children (aged 7-18 years). twenty of these molars were filled and sealed with a glass-ionomer cement (fuji ionomer type iii) and 4 with a resin sealant (delton). from each molar, two dentine samples were collected aseptically with a time interval of 7 months. the first sample wa ...19938242680
microbiology of the pericoronal pouch in mandibular third molar pericoronitis.the microorganisms associated with mandibular third molar pericoronitis were investigated using direct microscopy and anaerobic culture method. the pericoronal pouch was sampled with paper points in a) 8 patients without mandibular third molar pericoronitis and b) 6 patients with mandibular third molar pericoronitis. under the microscope, the microflora was found to be a complex mixture comprising gram-positive and gram-negative cocci, rods and filaments (including fusiform and curved rods), mot ...19938265205
the immediate and long-term effects of invasive and noninvasive pit and fissure sealing techniques on the microflora in occlusal fissures of human teeth.the purpose of this study was to evaluate the effect of acid conditioning and occlusal sealant on microbial colonization of pit and fissures submitted to ameloplasty or left intact. human enamel blocks containing fissures prepared from the occlusal surfaces of unerupted third molars were implanted in occlusal fillings in molars of 12 patients for seven, 30, 60, and 120 days. after seven days of exposure to the oral environment, the pit and fissure blocks were removed and found to be colonized, m ...19938327360
lung abscess: analysis of 66 cases from 1979 to 1991.sixty-six patients with bacterial lung abscess were treated between 1979 and 1991 in our hospital. among these patients, death occurred in one of the 42 cases of community-acquired infection (mortality rate: 2.4%) and in 16 of the 24 cases of nosocomial infection (mortality rate: 66.7%). of all 66 cases, 55 were culture-positive, and the etiologic agents isolated from 24 of the culture-positive cases were found to be anaerobic bacteria. the most common aerobes isolated from the foci were identif ...19938358116
analysis of intestinal flora of a patient with congenital absence of the portal vein.a 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having a congenital absence of the portal vein. the blood ammonia level (approximately 120 micrograms dl-1) in the superior mesenteric vein was markedly low compared to the normal value of 300-350 micrograms dl-1 in the portal vein. the decreased ammonia concentration and urease activity of the patient's faeces were demonstrated. the dominant intestinal flora in the faeces o ...19938364525
antibacterial effects of various endodontic medicaments on selected anaerobic bacteria.this study was undertaken to determine the antibacterial effects of various endodontic medicaments against six selected anaerobic bacteria. the experiment involved the testing of the vapors of six medicaments using agar plates streaked with the bacteria. a zone of inhibition was recorded for each plate and the results were analyzed statistically. formocresol produced significantly larger zones of inhibition than any of the other medicaments.19938120484
comparative evaluation of the rapid id 32a kit system, miniaturized standard procedure and a rapid fermentation procedure for the identification of anaerobic bacteria.the rapid id 32a and a rapid fermentation procedure were compared with a miniaturized standard procedure for the identification of 102 isolates of anaerobic bacteria belonging to 11 genera. the miniaturized standard procedure identified all 102 isolates at the genus level and 90 at the species level. the rapid fermentation procedure failed to identify 12 and 24 isolates at the genus and species levels respectively and is unsatisfactory. the rapid id 32a system misidentified 4 isolates (4%) at th ...19938392248
microbiology of early supragingival plaque development after delmopinol treatment.the aim of this double blind, cross-over, microbiological study was to compare the effect of topical application of the plaque control agent 0.5% delmopinol hcl with placebo on early supragingival plaque formation. six subjects underwent 7 periods (0.5, 1, 2, 8 and 24 h and 3 and 7 days) of placebo and delmopinol application, respectively. at the start of each study period the teeth were professionally cleaned and 2 ml of placebo and delmopinol 0.5%, respectively, were applied on all teeth (twic ...19938510982
anaerobic bacteria in orofacial abscesses.obligate anaerobic bacteria were cultured from 15 orofacial abscesses. bacteroides species constituted 43 per cent, clostridium 21 per cent, fusobacterium 14 per cent, peptostreptococci 11 per cent, peptococci 7 per cent and veillonella 4 per cent of the isolates. this study confirms the polymicrobial nature of orofacial infections. sensitivity to antibiotics was unpredictable. clostridium difficile, clostridium tetani, peptostreptococcus productus and veillonella parvula showed resistance to so ...19939508939
immunobiological activities of bacteria isolated from the root canals of postendodontic teeth with persistent periapical lesions.although intraradicular bacteria are widely considered to be the primary etiological agents of periapical lesions, the immunobiological properties of the species in question are not adequately known. the aim of this study, therefore, was to investigate the pathobiological properties of the 10 most frequently isolated endodontopathic bacterial species. using cellular components of the pathogens obtained by sonic extraction, we have investigated their ability to induce monocyte migration, interleu ...199219186419
the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction.the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ...19921318331
subgingival temperature (iii). relation to microbial counts.the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ...19921321846
sealing of occlusal hidden caries lesions: an alternative for curative treatment?the bacterial composition of the dentine of thirty molars with sealed occlusal surfaces (delton tinted) was examined. undiagnosed hidden caries was present at the time of treatment. although clinically assessed as good (age 3.4 years, s.d. 2.1), 47 percent of the sealants had a marginal defect, when assessed with sem. the dentine of the treated teeth was of a soft consistency. the median of the number of microorganisms was 400 (range 0-3.10(5)) cfu per sample. mutans streptococci and lactobacill ...19921430495
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
bacterial growth inhibition and short-term clinical efficacy of a vegetable oil-based mouthrinse: preliminary study.bacterial growth of a vegetable oil-based product was determined in vitro. growth of streptococcus mutans and veillonella alcalescens was strongly inhibited for dilutions up to 50x. on the basis of these results, it was decided to evaluate the short-term, clinical efficacy of a 1:10 dilution of the concentrated product in water. fifteen human volunteers employed this rinse twice-a-day during a six-day period. planimetric plaque scores, as well as the plaque and gingival indexes according to löe, ...19921499249
enumeration of subgingival species on primary isolation plates using colony lifts.this study evaluated the feasibility of using a colony lift method and dna probes to enumerate bacterial species cultured on primary isolation plates. fourteen digoxigenin-labeled whole chromosomal dna probes representing 12 subgingival species were validated by hybridization with colony lifts prepared from 249 reference strains of 51 species grown on trypticase soy agar plates supplemented with 5% sheep blood. colonies of reference strains were lifted onto nytran filters from plates and treated ...19921528619
the early colonization of gram-negative anaerobic bacteria in edentulous infants.the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ...19921528621
comparative mitogenicity and polyclonal b cell activation capacity of eight oral or nonoral bacterial lipopolysaccharides in cultures of spleen cells from athymic (nu/nu-balb/c) and thymic (balb/c) mice.optimal stimulatory doses of purified phenol-water extracted lipopolysaccharides (lps) from 5 selected strains of 3 putative periodontopathogens (fusobacterium nucleatum, prevotella intermedia, and veillonella), 3 strains of 2 nonoral bacterial species (bacteroides fragilis and salmonella enteritidis), and pokeweed mitogen (pwm) induced significantly higher maximum mitogenic responses and polyclonal ig production in cultures of unfractionated spleen cells from nu/nu-balb/c (nude) than from balb/ ...19921528635
modulation of human neutrophil adherence by oral bacteria.polymorphonuclear neutrophils (pmns) comprise over 90 per cent of leukocytes in the oral cavity. although these phagocytic cells have primary defence roles in the gingiva, their stimulation by micro-organisms may also cause substantial tissue damage due to the release of lysosomal enzymes and oxygen radicals. adherence of pmns to the endothelium and their subsequent diapedesis and egress to areas of infection are considered early vital events in the inflammatory process. in this study, oral bact ...19921605750
resistance of anaerobic bacteria to antimicrobial agents in spain.as a consequence of antibiotic consumption, the entire microbial ecosystem attached to man is evolving towards resistance. in spain, penicillin resistance (mic > 0.5 mg/l) is found in about 10% of peptostreptococcus, clostridium perfringens and eubacterium, and in 50% of veillonella. cefoxitin resistance ( > 2 mg/l) is present in 10 to 20% of peptostreptococcus and clostridium, and in 50% of eubacterium; 21% of bacteroides (fragilis group) strains are resistant to 16 mg/l. a very low rate of imi ...19921295754
[roxithromycin in dental infections].roxithromicin is a semisynthetic macrolide antibiotic, with similar antibacterial activity to erythromycin. it is characterized by an excellent pharmacokinetic profile and a good tissue penetration. particularly at the dose of 300 mg per day the dental tissue diffusion is extremely good, and this is important to oppose the oral cavity infections. oral cavity infections can be either odontogenic or non odontogenic. odontogenic infections are typically primary and are caused by commensal bacteria ...19921301492
pyogenic sacroiliitis due to veillonella parvula. 19921617061
microbiologic and pathologic aspects of pulpal and periapical disease.the greatest cause of endodontic and periapical pathosis is microbial infection of the pulp. most odontogenic infections are of a polymicrobial nature. with advances in anaerobic isolation and culturing techniques, much has been learned about the presence of pathogenic organisms such as porphyromonas and prevotella species (formerly classified as black-pigmented bacteroides species) in infected root canals. this review provides a summary of recent developments in endodontic microbiology, virulen ...19921381632
bacterial invasion of non-exposed dental pulp.anaerobic procedures were adopted to demonstrate the early bacterial invasion of non-exposed dental pulps, and to isolate and identify the bacteria. of 19 freshly extracted teeth which originally exhibited deep dentinal lesions, clinical examination and electric pulp testing showed that nine of them had no pulpal exposure. thus the pulps of these teeth were covered by clinically sound dentine beneath the carious lesion. bacteria were found to have invaded the pulps of six of these nine teeth. th ...19921399049
anaerobic microflora associated with the pars oesophagea of the pig.in healthy pigs aged one to 35 days significant populations of microorganisms (5.5 to 6.9 log10 viable count cm-2 of the tissue) were found, by strict anaerobic techniques, to adhere to the pars oesophagea. the genera of anaerobes which were isolated included actinomyces, bifidobacterium, clostridium, lactobacillus, peptostreptococcus, streptococcus and veillonella and facultative anaerobes included escherichia, klebsiella, staphylococcus, streptococcus and yeasts. the microbial population adher ...19921410807
complement activation by lipopolysaccharides purified from gram-negative bacteria isolated from infected root canals.the purpose of the present study was to investigate the complement activation by lipopolysaccharides purified from porphyromonas (bacteroides) endodontalis, veillonella parvula, and fusobacterium nucleatum isolated from infected root canals. the rate of consumption of the c3t component of the complement increased remarkably when lipopolysaccharides of more than 10 micrograms were added.19921437068
in vitro hydrogen production by enteric bacteria cultured from children with small bowel bacterial overgrowth.lactulose breath hydrogen test and enterotest string test were carried out simultaneously on 19 children 3-5 years old. bacteria isolated from the jejunal fluid in upper small intestines of these children were incubated with lactulose at neutral ph. anaerobes were present in all but one child, and in 15 children they were present in numbers greater than 5 log10 organisms per ml. most of these bacteria did not produce hydrogen in vitro. hydrogen production (greater than 100 ppm) was observed with ...19921534365
osteomyelitis due to veillonella parvula: case report and review.anaerobic gram-negative cocci of the genus veillonella are generally normal flora of the oropharynx and upper respiratory tract. when isolated in clinical specimens, these bacteria are usually regarded as commensal organisms rather than as pathogens. we report a case of spinal osteomyelitis in which veillonella parvula was isolated in pure culture from bone as well as blood. osteomyelitis of the spine due to anaerobic bacteria may occur more frequently than has been recognized, and anaerobic cul ...19921571454
effect of piperacillin/tazobactam therapy on intestinal microflora.the effect of piperacillin/tazobactam treatment upon the intestinal microflora was studied in 20 patients with intraabdominal infections. the patients received piperacillin 4 g combined with tazobactam 500 mg q 8 h by intravenous injection for 4-8 days. stool specimens were collected before, during and after therapy for cultivation of aerobic and anaerobic microorganisms. six patients had measurable concentrations of piperacillin (1.2-276 mg/kg/faeces) and 4 patients tazobactam concentrations (0 ...19921322559
experimentally induced infection by oral anaerobic microorganisms in a mouse model.a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ...19921408362
growth yield increase and atp formation linked to succinate decarboxylation in veillonella parvula.veillonella parvula strain 259 (= dsm 2007) was able to grow on a mineral salts medium supplemented with (per litre) 1 g yeast extract, 1 g tween-80, and 3 mg putrescine.2 hcl, with 6 mm thioglycolate as reductant and lactate as growth substrate. succinate did not serve as a growth substrate, but when added in conjunction with lactate, it was decarboxylated to propionate and resulted in a measurable increase in growth yield, corresponding to the formation of 2.4 g cell dry mass per mol succinate ...19921510569
characterization of veillonella atypica pk1910 adhesin-mediated coaggregation with oral streptococcus spp.the gram-negative human oral bacterium veillonella atypica pk1910 exhibits both lactose-inhibitable and lactose-noninhibitable coaggregations with certain human oral streptococci. a mild sonication procedure was used to obtain a veillonella surface protein preparation against which antisera were prepared. to characterize the lactose-inhibitable coaggregation, coaggregation-defective (cog-) mutants unable to exhibit this kind of coaggregation (class 1 mutants) were used to absorb the antisera. on ...19921541534
modelling of mixed chemostat cultures of an aerobic bacterium, comamonas testosteroni, and an anaerobic bacterium, veillonella alcalescens: comparison with experimental data.a mathematical model of mixed chemostat cultures of the obligately aerobic bacterium comamonas testosteroni and the anaerobic bacterium veillonella alcalescens grown under dual limitation of l-lactate and oxygen was constructed. the model was based on michaelis-menten-type kinetics for the consumption of substrates, with noncompetitive inhibition of v. alcalescens by o2. the growth characteristics of the aerobic and anaerobic organisms were determined experimentally with pure cultures of the ind ...19921622213
energy conservation by succinate decarboxylation in veillonella parvula.veillonella parvula cannot grow with succinate as sole energy source. however, succinate decarboxylation simultaneous with malate or lactate fermentation increased growth yields by 2.4-3.5 g (mol succinate)-1. malate was fermented stoichiometrically to acetate and propionate whereas lactate fermentation produced more acetate and considerable amounts of h2. aspartate was utilized only in the presence of succinate as co-substrate. methylmalonyl-coa decarboxylase and atp-dependent pyruvate carboxyl ...19921645132
relation of counts of microbial species to clinical status at the sampled site.the purpose of the present investigation was to relate clinical characteristics at a site to the frequency of detection, absolute counts and proportions of 14 subgingival species. subgingival plaque samples were removed by curette from the mesial surface of 2299 teeth in 3 healthy and 87 subjects with periodontal attachment loss. samples were dispersed, diluted and plated on trypticase soy agar supplemented with 5% sheep blood. after 7 days of anaerobic incubation, colonies were lifted onto nylo ...19911661305
a comparison of human and animal mouth flora.human bite injuries are often believed to be more serious than animal bite injuries. one reason for this distinction might be a species difference in the microbial mouth flora. in order to compare this possibility, we cultured the mouths of humans, dogs, and cats. human oral flora contained the smallest number of bacteria followed by dog and cat oral flora, respectively. veillonella parvula was cultured from all humans and was also found in dog and cat mouths, but less frequently. v parvula is n ...19911744782
[infective endocarditis and pulmonary abscess in an intravenous drug addict caused by veillonella parvula]. 19911784772
relation of baseline microbial parameters to future periodontal attachment loss.the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ...19911661304
a multi-station dental plaque microcosm (artificial mouth) for the study of plaque growth, metabolism, ph, and mineralization.a plaque growth chamber was developed for long-term growth of five separate plaques from the same plaque or saliva sample under identical conditions of temperature and gas phase. reagent addition and growth conditions for each plaque could be independently controlled, and each was accessible for sequential sampling and electrode insertion. plaques were cultured for over six weeks on pellicle-coated lux (tm) 25-mm diameter cover-slips at 35 degrees c under 5% co2 in n2, and supplied with a medium ...19911960250
prediction of caries increment in scottish adolescents.salivary, clinical, and microbiological factors were compared with subsequent 2-yr caries increment in a group of 372 scottish adolescents. the caries increment was significantly correlated with previous caries experience, salivary buffering capacity and counts of lactobacilli, mutans streptococci and candida. veillonella levels and salivary flow rate were not correlated with caries increment. significant improvements in the predictions were obtained when the results of more than one test were i ...19912049927
effect of nutrients on defined bacterial plaques and streptococcus mutans c67-1 implantation in a model mouth.actinomyces viscosus wvu 627, streptococcus oralis lpa-1 and veillonella dispar omz 193 were cocultured on teeth in a model mouth for 66 h. synthetic saliva containing bovine salivary glycoprotein supported bacterial growth, although the delivery of an intermittent nutrient supplement, containing 1% (w/v) glucose or sucrose, gave greater bacterial cell and viable counts. when streptococcus mutans c67-1 was super-inoculated onto 24-hour mixed plaques, it became established under all regimens, but ...19912059972
comparison of the plaque microflora from natural and appliance-borne enamel surfaces.human enamel sections and slabs, mounted on a mandibular removable appliance, were worn by 5 adult subjects for a 1-week period. plaque was allowed to accumulate on the in situ test sites and on the adjacent natural dentition. at the end of the experimental period, the plaque microflora associated with (1) the enamel sections, (2) the enamel slabs, and (3) the acrylic base of the appliance test site was compared with that obtained from lingual and interproximal areas of the lower molar teeth. in ...19912070382
microbial risk indicators for periodontal attachment loss. 19911831856
bacteria in the apical 5 mm of infected root canals.ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ...19911809801
an examination of strains of the bacterium streptococcus vestibularis for relative cariogenicity in gnotobiotic rats and adhesion in vitro.the cariogenicity and adhesion of six strains of streptococcus vestibularis were compared with those of strains of strep. salivarius. all strains of strep. vestibularis produced low levels of caries, confined to the fissures, whereas the two strains of strep. salivarius produced high levels of caries, with one strain producing approximal as well as fissure caries. the values for adhesion to saliva-coated hydroxyapatite of strep. vestibularis in the absence (median 4.74%) and the presence (median ...19911651693
biological effects of veillonella parvula and bacteroides intermedius lipopolysaccharides.a comparative study on the endotoxic effects of lipopolysaccharide (lps) from veillonella parvula atcc 10790 and from bacteroides intermedius bmh was performed using an in vivo approach in the c57bl/6 mouse. phenol-water extracted lps of such anaerobes was purified by ultracentrifugation and dnase/rnase digestion, and characterized by a metachromatic assay for endotoxins and by electrophoresis on sds-polyacrylamide gel and silver staining. mouse ld50 for v. parvula lps was 1.479 mg and for b. in ...19911775087
[effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections].the results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. in the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to bacteroides, fusobacterium, peptococcus, peptostreptococcus, veillonella and actinomyces. significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. there was observed high sensitivity of the obli ...19911805697
predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
[oral bacterial flora and its pathogenic potential].the oral flora is a complex body formed by at least a hundred bacterial species which can be more or less regular residents in the oral cavity. thanks to a developing taxonomy related to progress made in bacterial identification, these oral species can be separated into five groups based gram staining and morphology: the gram positive cocci group (streptococcus, staphylococcus and related geni), the gram negative cocci group (neisseria, veillonella and related geni), the gram positive bacilli gr ...19911864855
swedish study group. a randomized multicenter trial to compare the influence of cefaclor and amoxycillin on the colonization resistance of the digestive tract in patients with lower respiratory tract infection.eighty-four patients with lower respiratory tract infections participated in a randomised double-blind parallel multicenter trial in order to compare the efficacy of cefaclor and amoxycillin as treatment for lower respiratory tract infections and their ability to influence colonization resistance. cefaclor was given to 40 patients and amoxycillin to 44 patients perorally in doses of 250 mg t.i.d. for seven days in a double-blind fashion. sputum, oropharyngeal and intestinal specimens were taken ...19911917031
[pleural empyema and ruptured subdiaphragmatic abscess].the authors report a case of a ruptured right subphrenic abscess causing a diffuse peritonitis complicating a chronic empyema. the authors recommend active treatment of purulent pleurisies, particularly when they are associated with a bronchopleural fistula.19911924980
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
specificity of antibodies present in human periapical lesions.various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ...19911940740
on the mechanism of sodium ion translocation by methylmalonyl-coa decarboxylase from veillonella alcalescens.veillonella alcalescens during lactate degradation developed an na+ concentration gradient with 7-8 times higher external than internal na+ concentrations in the logarithmic growth phase. the gradient declined to a factor of 1.9 in the late stationary phase. methylmalonyl-coa decarboxylase reconstituted into proteoliposomes performed an active electrogenic na+ transport, creating delta psi of 60 mv, delta pna+ of 50 mv, and delta mu na+ of 110 mv. in the initial phase of the transport, the decar ...19911991479
Displaying items 401 - 500 of 1130