Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness.the centers for disease control and prevention and united states army research institute for infectious diseases have developed real-time pcr assays for the detection of bioterrorism threat agents. these assays all rely on a limited number of approved real-time pcr master mixes. because the availability of these reagents is a critical element of bioterrorism preparedness, we undertook a joint national preparedness exercise to address the potential surge needs resulting from a large-scale bio-eme ...201223107058
evaluation of sample preparation methods for maldi-tof ms identification of highly dangerous bacteria.to propose a universal workflow of sample preparation method for the identification of highly pathogenic bacteria by maldi-tof ms.201222512320
misidentification of brucella melitensis as bergeyella zoohelcum by microscan walkaway®: a case report.to describe the misidentification of brucella melitensis as bergeyella zoohelcum by microscan walkaway®, a commonly used bacterial identification system.201222614245
16s rrna gene sequence analysis of a brucella melitensis infection misidentified as bergeyella zoohelcum.misidentification of brucella species from clinical specimens using commercial bacterial identification systems is a recurring problem. an isolate from a bacterimic patient was identified as bergeyella zoohelcum by microscan walk-away (siemens healthcare diagnostics inc., west sacramento, ca, usa) and as brucella melitensis by vitek 2 system (biomérieux inc., durham, nc, usa). because of this identification ambiguity by the two automated bacterial identification systems we performed 16s rrna seq ...201222421611
transcriptome analysis of hela cells response to brucella melitensis infection: a molecular approach to understand the role of the mucosal epithelium in the onset of the brucella pathogenesis.brucella spp. infect hosts primarily by adhering and penetrating mucosal surfaces, however the initial molecular phenomena of this host:pathogen interaction remain poorly understood. we hypothesized that characterizing the epithelial-like human hela cell line molecular response to wild type brucella melitensis infection would help to understand the role of the mucosal epithelium at the onset of the brucella pathogenesis. rna samples from b. melitensis-infected hela cells were taken at 4 and 12 h ...201222484383
bacteriological features of infectious spondylodiscitis at mohammed v military teaching hospital of rabat.to review the bacteriological features of infectious spondylodiscitis and provide recommendations for the initial therapy which remains empirical in our context. retrospective study including patients diagnosed with spondylodiscitis over a period of 4 years (2006-2009) at the rabat military teaching hospital. during the study period, we analysed 30 cases: the mean age was 49.9 years and 21 cases (70%) were male. the patients were predominantly hospitalized in neurosurgery department (15/30) foll ...201224031960
factors associated with seropositive antibodies to brucella melitensis in the nakhon nayok, thailand.human brucellosis is a re-emerging disease in thailand. in 2006, her royal highness princess maha chakri sirindhorn medical center nakorn nayok province had diagnosed three cases of brucellosis which have never been reported in this area.201223513464
identification of the virb operon genes encoding the type iv secretion system, in colombian brucella canis isolates.canine brucellosis is a zoonotic disease caused by brucella canis. the establishment of intracellular replicative niches of b. canis is mediated by proteins secreted by the type iv secretion system, which is encoded by the virb operon. the characterization of such genes has been conducted in other species of the genus, but not in b. canis. we report the design of a multiplex pcr test for the detection of the virb operon genes of b. canis. primers for each of the 12 genes were designed and evalua ...201223290573
seroprevalence of brucellosis in livestock within three endemic regions of the country of georgia.brucellosis is the one of most common livestock zoonoses in georgia, resulting in significant economic losses. livestock were sampled in three regions of georgia (kakheti, kvemo kartli, imereti). districts that historically reported high numbers of brucellosis related morbidity were selected for serological, bacteriological and molecular surveys. surveying efforts yielded samples from 10,819 large and small ruminants. in total, 735 serological tests were positive on rose bengal and 33 bacterial ...201223287714
safety and efficacy of reduced doses of brucella melitensis strain rev. 1 vaccine in pregnant iranian fat-tailed ewes.brucellosis is one of the most important zoonotic diseases and is a significant cause of abortion in animals. brucella melitensis strain rev. 1 is recommended as the most effective vaccine for small ruminants but the application of full doses in adult animals is restricted. this study was conducted to determine a proper reduced dose of vaccine which confers protection but which is not abortifacient in iranian fat-tailed sheep. a total of 51 non-vaccinated pregnant ewes were divided into three ma ...201223277121
cell mediated immune response after challenge in omp25 liposome immunized mice contributes to protection against virulent brucella abortus 544.brucellosis is a disease affecting various domestic and wild life species, and is caused by a bacterium brucella. keeping in view the serious economic and medical consequences of brucellosis, efforts have been made to prevent the infection through the use of vaccines. cell-mediated immune responses [cmi] involving interferon gamma and cytotoxic cd4(+) and cd8(+) t cells are required for removal of intracellular brucella. omp25 has been reported to be involved in virulence of brucella melitensis, ...201223273966
predicting antigenicity of proteins in a bacterial proteome; a protein microarray and naïve bayes classification approach.discovery of novel antigens associated with infectious diseases is fundamental to the development of serodiagnostic tests and protein subunit vaccines against existing and emerging pathogens. efforts to predict antigenicity have relied on a few computational algorithms predicting signal peptide sequences (signalp), transmembrane domains, or subcellular localization (psort). an empirical protein microarray approach was developed to scan the entire proteome of any infectious microorganism and empi ...201222589097
unusual presentation of neurobrucellosis: a solitary intracranial mass lesion mimicking a cerebral tumor : a case of encephalitis caused by brucella melitensis.among the diverse presentations of neurobrucellosis, solitary intracranial mass lesions are extremely rare. to the best of our knowledge, we describe here the second case of neurobrucellosis mimicking a cerebral tumor caused by brucella melitensis. the mass lesion was clinically and radiologically indistinguishable from a brain tumor. the diagnosis was established by isolating brucella melitensis in a blood culture and a positive wright's agglutination test on the cerebrospinal fluid at 1:320 ti ...201222231602
yersinia enterocolitica serotype o:9 cultured from swedish sheep showing serologically false-positive reactions for brucella melitensis.in a herd of 20 sheep in sweden, a country where brucellosis has never been diagnosed in sheep or goats, a total of six sheep were found serologically positive to brucella melitensis in two different rounds of sampling. yersinia enterocolitica serotype o:9 could at the time of the second sampling be isolated from four sheep, one of them at the same time serologically positive for b. melitensis. the article describes the case and gives some background information on brucellosis and y. enterocolit ...201223240071
crucial role of gamma interferon-producing cd4+ th1 cells but dispensable function of cd8+ t cell, b cell, th2, and th17 responses in the control of brucella melitensis infection in mice.brucella spp. are facultative intracellular bacterial pathogens responsible for brucellosis, a worldwide zoonosis that causes abortion in domestic animals and chronic febrile disease associated with serious complications in humans. there is currently no approved vaccine against human brucellosis, and antibiotic therapy is long and costly. development of a safe protective vaccine requires a better understanding of the roles played by components of adaptive immunity in the control of brucella infe ...201223006848
mlva16 typing of portuguese human and animal brucella melitensis and brucella abortus isolates.to investigate the epidemiological relationship of isolates from different portuguese geographical regions and to assess the diversity among isolates, the mlva16(orsay) assay (panels 1, 2a and 2b) was performed with a collection of 126 brucella melitensis (46 human and 80 animal isolates) and 157 b. abortus field isolates, seven vaccine strains and the representative reference strains of each species. the mlva16(orsay) showed a similar high discriminatory power (hgdi 0.972 and 0.902) for both sp ...201222905141
ifn-γ-deficient mice develop il-1-dependent cutaneous and musculoskeletal inflammation during experimental brucellosis.human brucellosis exhibits diverse pathological manifestations that can affect almost any organ. in particular, osteoarticular complications are the most common focal manifestation of brucellosis and occur in 40-80% of patients. in immunocompetent mice, brucella replication is generally restricted to the spleen, liver, and to a lesser extent, lns, thereby limiting their use for study of focal inflammation often found in brucellosis. here, we report that nasal, oral, or peritoneal infection of if ...201222636321
a rare case of seronegative culture--proven infection with brucella suis.brucellosis is a chronic infection produced by members of the brucella family. diagnosis of this condition requires either isolation of the organism in culture or positive serological tests.we describe a 27-year-old male admitted as a case of pyrexia of unknown origin (puo), who tested negative for brucella igm elisa test on preliminary evaluation but was subsequently diagnosed on the strength of positive blood and bone marrow cultures to be a case of brucellosis secondary to brucella suis infec ...201222905059
surgical management of cervical spinal epidural abscess caused by brucella melitensis : report of two cases and review of the literature.spinal epidural abscess, if especially caused by brucellosis is a very rare disease which is usually a consequence of spondylodiscitis. the spinal column can be affected at any joint; however, the lumbar spine is the most common region, especially at the level of the l4-5 and l5-s1. the frequency of spinal involvement usually seen at the lumbar, thoracic and cervical spine respectively. as an occupational disease in farmers, veterinaries, butchers, laboratory staff and shepherds, brucellosis can ...201222949972
in situ microscopy analysis reveals local innate immune response developed around brucella infected cells in resistant and susceptible mice.brucella are facultative intracellular bacteria that chronically infect humans and animals causing brucellosis. brucella are able to invade and replicate in a broad range of cell lines in vitro, however the cells supporting bacterial growth in vivo are largely unknown. in order to identify these, we used a brucella melitensis strain stably expressing mcherry fluorescent protein to determine the phenotype of infected cells in spleen and liver, two major sites of b. melitensis growth in mice. in b ...201222479178
serial kinetics of the antibody response against the complete brucella melitensis orfeome in focal vertebral brucellosis.human brucellosis is a common zoonosis worldwide. here we present a case of focal vertebral brucellosis in a 71-year-old mexican-american woman who contracted infection from unpasteurized goat milk. standard agglutination serology was negative; the diagnosis was established by the isolation of brucella melitensis from abscess fluid. a b. melitensis protein microarray comprised of nearly all proteins encoded by the bacterial genome was used to determine the kinetics of this patient's antibody res ...201222219303
simultaneous detection and differentiates of brucella abortus and brucella melitensis by combinatorial pcr.to evaluate simultaneous detection and differentiates of brucella abortus (b. abortus) and brucella melitensis (b. melitensis) through the combinatorial pcr method.201222182638
pathophysiology of the rhesus macaque model for inhalational brucellosis.the objective of this study was to characterize the rhesus macaque (rm) as a model for inhalational brucellosis in support of the u.s. food and drug administration's (fda) animal rule. the pathophysiology of chronic brucella melitensis aerosol infection was monitored in two phases that each occurred over an 8-week time period; dose escalation (8 rms; targeted doses of 5.0e+03, 5.0e+04, or 5.0e+05 cfu/animal or the unchallenged control) and natural history (12 rms; targeted dose of 2.50e+05 cfu/a ...201222064715
Acellular vaccines for ovine brucellosis: a safer alternative against a worldwide disease.Ovine brucellosis is a very contagious zoonotic disease distributed worldwide and constitutes a very important zoosanitary and economic problem. The control of the disease includes animal vaccination and slaughter of infected flocks. However, the commercially available vaccine in most countries is based on the attenuated strain Brucella melitensis Rev 1, which presents important safety drawbacks. This review is focused on the most recent and promising acellular vaccine proposals.201222149711
a large exposure to brucella melitensis in a diagnostic laboratory.background: brucella species are easily transmitted by aerosols and can be acquired in the laboratory. aim: to report the management of a large exposure to brucella melitensis that occurred over six days in a hospital diagnostic laboratory. methods: fifty-one exposed staff were managed according to centers for disease control and prevention guidelines. a further 96 non-exposed laboratory staff were tested for seroprevalence. testing was carried out using the brucella sp. serum agglutination test ...201222237130
lymphocyte activation test for diagnosis of seronegative brucellosis in humans.background: seronegative brucellosis is occasionally encountered in clinical practice especially in localized disease where diagnosis is reached mainly through positive blood culture. cellular immune responses are pivotal for protection against intracellular bacteria such as brucella. this study was performed to evaluate the expression of activation markers on peripheral blood mononuclear cells in response to in vitro stimulation by whole-cell suspension of brucella melitensis for the diagnosis ...201122234109
[spondylodiscitis: which etiology?].spondylodiskitis is a rare condition in adults but with high index of morbidity and mortality due to difficult diagnosis. the insidious evolution and the unspecificity of symptoms interfere with prompt treatment, being the diagnosis based primarily on a high index of suspicion. there are two major groups of spondylodiskitis: pyogenic, being staphylococcus aureus the most frequent etiological pathogen, and granulomatous, caused mainly by mycobacterium tuberculosis and brucella melitensis, both en ...201122713202
the first international standard anti-brucella melitensis serum.the world organisation for animal health (oie) requested an international standard anti-brucella melitensis serum (isabms) to standardise diagnostic tests and reagents for sheep and goats. the agreed criteria were the highest dilution (in negative serum) of the standard which must give a positive result and the lowest dilution (in negative serum) which must simultaneously give a negative result. the two dilutions for each assay were, respectively: indirect enzyme-linked immunosorbent assay (ieli ...201122435193
characterization of outer membrane vesicles from brucella melitensis and protection induced in mice.the outer membrane vesicles (omvs) from smooth b. melitensis 16 m and a derived rough mutant, vtrm1 strain, were purified and characterized with respect to protein content and induction of immune responses in mice. proteomic analysis showed 29 proteins present in omvs from b. melitensis 16 m; some of them are well-known brucella immunogens such as sod, groes, omp31, omp25, omp19, bp26, and omp16. omvs from a rough vtrm1 induced significantly higher expression of il-12, tnfα, and ifnγ genes in bo ...201122242036
deep-sequencing analysis of the mouse transcriptome response to infection with brucella melitensis strains of differing virulence.brucella melitensis is an important zoonotic pathogen that causes brucellosis, a disease that affects sheep, cattle and occasionally humans. b. melitensis strain m5-90, a live attenuated vaccine cultured from b. melitensis strain m28, has been used as an effective tool in the control of brucellosis in goats and sheep in china. however, the molecular changes leading to attenuated virulence and pathogenicity in b. melitensis remain poorly understood. in this study we employed the illumina genome a ...201122216095
brucellosis presenting as septic shock.brucellosis generally presents with fever, malaise, weight loss and bone pain with either an abrupt or insidious onset. a 76-year-old man presented in april 2010 with fever of 103°f, severe tachycardia, tachypnoea and a blood pressure of 80/50 mm hg requiring fluids and vasopressor support with norepinephrine. the patient had brucellosis in 1956 which was treated for 'many weeks' with tetracycline and streptomycin. he has had no recurrences since that time. he denies recent travel outside the us ...201122701076
brucella arthritis of the knee, 1 year after revision of anterior cruciate ligament reconstruction.brucellosis is a zoonotic infection with a broad spectrum of clinical manifestations. the authors report the first case in the literature of septic arthritis of the knee 1 year after revision of anterior cruciate ligament reconstruction. brucella melitensis biotype 3 was found in both synovial fluid and blood cultures. the patient was treated initially with arthroscopic debridement. after the diagnosis was confirmed, a second arthroscopic lavage and metal work removal was applied leaving the gra ...201122700607
extrapulmonary tuberculosis: importance of differential diagnosis.patients with tuberculosis (tb) typically present with pulmonary tb, but extrapulmonary tb is also an important clinical problem. in particular, osteoarticular tb has a great morbidity and its diagnosis gold-standard includes culture and histopathology of the infected area. the present case study describes a 53-year-old female portuguese farmer who had initial clinical and imaging features suggestive of tuberculous osteomyelitis. however, she denied any tb contacts and the tuberculin skin-test a ...201122679255
comparative analysis of glutaredoxin domains from bacterial opportunistic pathogens.glutaredoxin proteins (glxrs) are essential components of the glutathione system that reductively detoxify substances such as arsenic and peroxides and are important in the synthesis of dna via ribonucleotide reductases. nmr solution structures of glutaredoxin domains from two gram-negative opportunistic pathogens, brucella melitensis and bartonella henselae, are presented. these domains lack the n-terminal helix that is frequently present in eukaryotic glxrs. the conserved active-site cysteines ...201121904064
Molecular typing of Brucella melitensis endemic strains and differentiation from the vaccine strain Rev-1.In the present study forty-four Greek endemic strains of Br. melitensis and three reference strains were genotyped by Multi locus Variable Number Tandem Repeat (ML-VNTR) analysis based on an eight-base pair tandem repeat sequence that was revealed in eight loci of Br. melitensis genome. The forty-four strains were discriminated from the vaccine strain Rev-1 by Restriction Fragment Length Polymorphism (RFLP) and Denaturant Gradient Gel Electrophoresis (DGGE). The ML-VNTR analysis revealed that en ...201122021041
Brucella melitensis cyclic di-GMP phosphodiesterase BpdA controls expression of flagellar genes.Brucella melitensis encounters a variety of conditions and stimuli during its life cycle--including environmental growth, intracellular infection, and extracellular dissemination--which necessitates flexibility of bacterial signaling to promote virulence. Cyclic-di-GMP is a bacterial secondary signaling molecule that plays an important role in adaptation to changing environments and altering virulence in a number of bacteria. To investigate the role of cyclic-di-GMP in B. melitensis, all 11 pred ...201121856843
long-term survival of salvage cardiac transplantation for infective endocarditis.cardiac transplantation has been rarely performed in patients with infective endocarditis. a 31-year-old man developed aortic endocarditis due to brucella melitensis. he presented with fever and developed acute myocardial infarct, severe aortic regurgitation, and heart failure. aortic valve replacement did not improve cardiac function; hence, an emergent cardiac transplantation was carried out. eighteen years later, he is doing well and living an active and productive life. only 6 patients have ...201122051318
Survey to determine the seroprevalence of Toxoplasma gondii infection in British sheep flocks.A serological survey of Toxoplasma gondii infection in adult breeding sheep in Great Britain was conducted using surplus sera taken during a seroprevalence study of Brucella melitensis in 2009. Of the 3539 sera collected from 227 flocks, 2619 (74 per cent) were found to be positive for T gondii specific antibody when tested using latex agglutination. Multilevel logistic modelling suggested that the likelihood of infection increased with age and this effect appeared to be amplified in animals vac ...201121957115
acute brucellosis with myelodysplastic syndrome presenting as pancytopenia and fever of unknown origin.objective: to report a rare case of brucellosis with myelodysplastic syndrome (mds). clinical presentation and intervention: a 70-year-old woman presented with pancytopenia and fever of unknown origin (fuo). the initial diagnosis was brucellosis; the woman was treated with doxycycline and rifampin against brucella melitensis but was later diagnosed as suffering from mds. she was immediately transferred to the department of hematology for further evaluation. conclusion: this study highlights the ...201122133749
identification of immunoreactive proteins of brucella melitensis by immunoproteomics.infection with brucella causes brucellosis, a chronic disease in humans, which induces abortion and sterility in livestock. among the different brucella species, brucella melitensis is considered the most virulent and is the predominant species associated with outbreaks in china. to date, no safe human vaccine is available against brucella infection. the currently used live vaccines against brucella in livestock induce antibodies that interfere with the diagnosis of field infection in vaccinated ...201121922434
Acute renal failure due to Brucella melitensis.We present the case of a 42-year-old male patient who applied to the emergency department of our hospital with clinical nephritis, orchitis, acute renal failure without endocarditis, and a low-grade fever. Brucella agglutinin titers were 1:160, Rose Bengal test was positive and Brucella melitensis was isolated from urine and blood cultures.. A combination of oral rifampin (600 mg/day) and doxycycline (200 mg/day) was administered along with supportive treatment leading to resolution of his clini ...201122169790
Interactions of the human pathogenic Brucella species with their hosts.Brucellosis is a zoonotic infection caused primarily by the bacterial pathogens Brucella melitensis and B. abortus. It is acquired by consumption of unpasteurized dairy products or by contact with infected animals. Globally, it is one of the most widespread zoonoses, with 500,000 new cases reported each year. In endemic areas, Brucella infections represent a serious public health problem that results in significant morbidity and economic losses. An important feature of the disease is persistent ...201121939378
mlva genotyping of chinese human brucella melitensis biovar 1, 2 and 3 isolates.since 1950, brucella melitensis has been the predominant strain associated with human brucellosis in china. in this study we investigated the genotypic characteristics of b. melitensis isolates from china using a multiple-locus variable-number tandem-repeat analysis (mlva) and evaluated the utility of mlva with regards to epidemiological trace-back investigation.201122108057
[comparison of conventional methods and real-time multiplex polymerase chain reaction for identification and typing of brucella isolates of human origin].brucellosis which is a worldwide zoonotic disease, still constitutes a major public health problem in rural areas of turkey. the aim of the present study was to evaluate the species and biovar distribution of 187 presumptive brucella strains isolated from patients inhabiting at the provinces in eastern, south eastern and mediterranean regions over a 7-years period (from 2001 to 2007) and to compare multiplex real-time-polymerase chain reaction (m-rt-pcr) and conventional biotyping for the differ ...201121935772
brucella melitensis infection within warthin tumor of the parotid gland.brucellosis is a zoonotic systemic infectious disease, and multiorgan involvement is commonly seen, but involvement of the neck is a rare presentation of brucellosis. granulomatous infections of the parotid gland are extremely rare. warthin tumor is a well-known benign neoplasm of the salivary glands. in this report, we describe a warthin tumor associated with brucella melitensis in the same parotid gland.201121959459
limitations of the standard agglutination test for detecting patients with brucella melitensis bacteremia.abstract the sensitivity of the standard agglutination test (sat) for detecting brucellosis was determined in 264 israeli patients from whom a positive blood culture for brucella melitensis and serology were obtained within±1 week. a sat titer ≥1:160 had a diagnostic sensitivity of 91.7%, whereas raising the cutoff to ≥1:320, as recommended to decrease false-positive rates in endemic areas, reduced the sensitivity to 82.6%. physicians working in regions endemic for brucellosis should be aware ...201121919731
The Brucella TIR-like protein TcpB interacts with the death domain of MyD88.The pathogen Brucella melitensis secretes a Toll/interleukin-1 receptor (TIR) domain containing protein that abrogates host innate immune responses. In this study, we have characterized the biochemical interactions of Brucella TIR-like protein TcpB with host innate immune adaptor proteins. Using protein-fragment complementation assays based on Gaussia luciferase and green fluorescent protein, we find that TcpB interacts directly with MyD88 and that this interaction is significantly stronger than ...201122155231
Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis.Brucella melitensis is the etiological agent responsible for brucellosis. Present in the B. melitensis genome is a 116-residue protein related to arsenate reductases (Bm-YffB; BR0369). Arsenate reductases (ArsC) convert arsenate ion (H(2)AsO(4)(-)), a compound that is toxic to bacteria, to arsenite ion (AsO(2)(-)), a product that may be efficiently exported out of the cell. Consequently, Bm-YffB is a potential drug target because if arsenate reduction is the protein's major biological function t ...201121904062
chronic, progressive back pain, fever and a noticeable paravertebral mass. 201121363970
imported brucellosis in denmark: molecular identification and multiple-locus variable number tandem repeat analysis (mlva) genotyping of the bacteria.a polymerase chain reaction was used to identify brucella species isolated from humans in denmark. consecutive analysis of referred bacteria and re-examination of historical isolates identified all as brucella melitensis. multiple-locus variable number tandem repeat analysis (mlva) placed the isolates in the previously defined 'east mediterranean' b. melitensis group.201121375425
brucellosis in pregnancy.this study was undertaken in order to evaluate the effect of brucellosis in pregnancy. the serum agglutination test, coombs and/or blood culture systems were used in the diagnosis of brucellosis. from july 2003 to september 2010, the clinical and delivery patterns of 40 healthy pregnant women were compared with 39 pregnant women who had brucellosis. there were no birth defects, anomalies or mortalities. we observed that brucellosis in pregnancy increases the incidence of preterm delivery (p = 0. ...201121378061
an analysis of children with brucellosis associated with pancytopenia.brucellosis produces a variety of nonspecific hematologic abnormalities. hematologic complications of mild anemia and leukopenia have been frequently associated with acute brucellosis, but pancytopenia is less frequently seen. in this study, records of children with brucellosis aged under or equal to 16 years, admitted to yuzuncu yil university hospital between 2004 and 2010, were analyzed retrospectively. over this time period, 187 patients with brucellosis were diagnosed. twenty-five (13.3%) o ...201121381870
association between brucella melitensis dna and brucella sp. antibodies. 201121411601
peculiarities of the regulation of the brucella flagellum. 201121415118
modulation of microtubule dynamics by a tir domain protein from the intracellular pathogen, brucella melitensis.tir domain containing proteins play a crucial role in innate immunity in eukaryotes. brucella is a highly infectious intracellular bacterium that encodes a tir domain protein (tcpb) to subvert host innate immune responses to establish a beneficial niche for pathogenesis. tcpb inhibits nf-+¦b activation and pro-inflammatory cytokine secretions mediated by toll like receptor (tlr) 2 & 4. here, we demonstrate that tcpb modulates microtubule dynamics by acting as a stabilization factor. tcpb increas ...201121692747
isolation rates of brucella melitensis in an endemic area and implications for laboratory safety.a retrospective study was conducted to assess the potential threat posed by processing blood cultures to clinical microbiology laboratory personnel working in an area endemic for brucella melitensis in southern israel. the computerized laboratory records for the 2002-2009 period were reviewed, and the proportion of aerobic vials from which brucella organisms were isolated out of the total number of positive aerobic blood culture vials was determined. during the 8-year period, b. melitensis was i ...201121725863
epidemiological survey of rifampicin resistance in clinic isolates of brucella melitensis obtained from all regions of turkey.the aim of the present study was to assess the antimicrobial susceptibility of brucella melitensis isolates to rifampicin (rif) depending on time and regional differences. a total of 94 human brucella isolates collected in an 8-year period from the beginning of 2002 to the end of 2009 throughout turkey were investigated. the isolates were identified at species and biovar levels by conventional methods, and minimum inhibitory concentrations (mic) of rif was determined by using the e test method. ...201121826587
protective efficacy and safety of brucella melitensis 16m{delta}mucr against intraperitoneal and aerosol challenge in balb/c mice.brucellosis is a zoonosis of nearly worldwide distribution. vaccination against this pathogen is an important control strategy to prevent the disease. currently licensed vaccine strains used in animals are unacceptable for human use due to undesirable side effects and modest protection. substantial progress has been made during the past 10 years toward the development of improved vaccines for brucellosis. in part, this has been achieved by the identification and characterization of live attenuat ...201121708998
brucella melitensis infection following military duty in iraq.brucellosis is a common zoonotic disease worldwide; however, few cases are reported in the us. brucella melitensis infections are primarily acquired via consumption of high-risk foods or travel to endemic areas. we describe a case of b. melitensis infection in a tennessee soldier following deployment in iraq. initial symptoms included knee and back pain. culture of an aspirate of the left sacroiliac joint yielded b. melitensis. genetic analysis indicated that this isolate came from the middle ea ...201121824352
extended safety and efficacy studies of the attenuated brucella vaccine candidates 16m{delta}vjbr and s19{delta}vjbr in the immunocompromised irf-1-/- mouse model.the global distribution of brucellosis and high incidence in certain areas of the world warrants the development of a safer and efficacious vaccine. for the past ten years, we have focused our attention on the development of a safer, but still highly protective, live-attenuated vaccine for human and animal use. we have demonstrated the safety and protective efficacy of the vaccine candidates 16mδvjbr and s19δvjbr against homologous and heterologous challenge in multiple immunocompetent animal mo ...201122169089
structure of fructose bisphosphate aldolase from bartonella henselae bound to fructose 1,6-bisphosphate.fructose bisphosphate aldolase (fbpa) enzymes have been found in a broad range of eukaryotic and prokaryotic organisms. fbpa catalyses the cleavage of fructose 1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. the ssgcid has reported several fbpa structures from pathogenic sources, including the bacterium brucella melitensis and the protozoan babesia bovis. bioinformatic analysis of the bartonella henselae genome revealed an fbpa homolog. the b. henselae fbpa enzym ...201121904049
Immunoproteomic analysis of Brucella melitensis and identification of a new immunogenic candidate protein for the development of brucellosis subunit vaccine.In order to screen immunogenic candidate antigens for the development of a brucellosis subunit vaccine, an immunoproteomic assay was used to identify immunogenic proteins from Brucella melitensis 16 M soluble proteins. In this study, a total of 56 immunodominant proteins were identified from the two-dimensional electrophoresis immunoblot profiles by liquid chromatography tandem mass spectrometry (LC-MS/MS). Two proteins of interest, riboflavin synthase alpha chain (RS-a) and Loraine synthase (LS ...201121943783
BrabA.11339.a: anomalous diffraction and ligand binding guide towards the elucidation of the function of a 'putative ß-lactamase-like protein' from Brucella melitensis.The crystal structure of a ß-lactamase-like protein from Brucella melitensis was initially solved by SAD phasing from an in-house data set collected on a crystal soaked with iodide. A high-resolution data set was collected at a synchroton at the Se edge wavelength, which also provided an independent source of phasing using a small anomalous signal from metal ions in the active site. Comparisons of anomalous peak heights at various wavelengths allowed the identification of the active-site metal i ...201121904058
systems biology approach predicts antibody signature associated with brucella melitensis infection in humans.a complete understanding of the factors that determine selection of antigens recognized by the humoral immune response following infectious agent challenge is lacking. here we illustrate a systems biology approach to identify the antibody signature associated with brucella melitensis (bm) infection in humans and predict proteomic features of serodiagnostic antigens. by taking advantage of a full proteome microarray expressing previously cloned 1406 and newly cloned 1640 bm genes, we were able to ...201121863892
Brucella melitensis Survival During Manufacture of Ripened Goat Cheese at Two Temperatures.Abstract The aim of the current work was to assess the influence of two temperatures, 4°C and 24°C, on pH and water activity and their association with Brucella melitensis survival during the traditional manufacture of ripened goat cheese. Raw milk from a brucellosis-free goat herd was used for the manufacture of ripened cheese. The cheese was inoculated with 5×10(9) of the B. melitensis 16M strain during the tempering stage. The cheeses were matured for 5, 20, and 50 days at both temperatures ...201122129442
roles of genomic island 3 (gi-3) bab1_0278 and bab1_0263 open reading frames (orfs) in the virulence of brucella abortus in balb/c mice.the genomic island 3 (gi-3) shared by brucella melitensis and brucella abortus contains 29 genes encoding mostly unknown proteins. within this island, the open reading frames (orfs) bab1_0278 and bab1_0263 are present, bab1_0278 encodes a hypothetical protein of 64 amino acids sharing a domain with the gcra superfamily, whereas the amino acid sequence of bab1_0263 showed 42% identity with an iron regulated lsr2 protein. we obtained one deletion mutant for each one of these orfs present within th ...201122005180
[chronic brucellosis: a rare cause of septic loosening of arthroplasties with high risk of laboratory-acquired infections].brucella spp. are a main contributor to morbidity in endemic countries, especially in the near and middle east and the mediterranean. however, in most western industrialised countries, cases are rare. here, we report the case of a previously unrecognised chronic brucellosis with subsequent laboratory-acquired infection. a 64-year-old female presented to the orthopaedic department with loosening of her knee prosthesis after total knee arthroplasty five years earlier. bacterial cultures were obtai ...201120419627
management of an outbreak of brucellosis due to b. melitensis in dairy cattle in spain.brucella melitensis is a major human and animal pathogen, with a wide host range that includes all domestic ruminant species, although small ruminants are its preferred hosts. outbreaks in cattle due to b. melitensis have become a worldwide emerging problem particularly difficult to control due to the lack of knowledge on the epidemiology in this host species and of an effective vaccine. however, combination of molecular tools and strict biosecurity measures can help to solve these difficulties ...201120579679
brucella melitensis: a rare cause of febrile neutropenia.although brucellosis is endemic in middle eastern and mediterranean countries, febrile neutropenia caused by brucella species has rarely been reported in childhood. in this report the authors described an unusual case of febrile neutropenia due to brucella melitensis.201120863159
antimicrobial susceptibility of brucella melitensis isolates in peru.brucellosis is an important public health problem in peru. we evaluated 48 human brucella melitensis biotype 1 strains from peru between 2000 and 2006. mics of isolates to doxycycline, azithromycin, gentamicin, rifampin, ciprofloxacin, and trimethoprim-sulfamethoxazole were determined by the etest method. all isolates were sensitive to tested drugs during the periods of testing. relapses did not appear to be related to drug resistance.201121199926
control and eradication of brucella melitensis infection in sheep and goats.brucella melitensis is the main etiological agent of brucellosis in sheep and goats, and is also the main agent responsible for human brucellosis, a predominantly occupational disease related to professions in direct contact with livestock. as there is currently no viable method of preventing human brucellosis to safeguard people attention must be directed toward effectively controlling the disease in sheep and goats. this review focuses on the different strategies in different socioeconomic and ...201121215893
assessment of milk ring test and some serological tests in the detection of brucella melitensis in syrian female sheep.brucella melitensis infection prevalence among syrian female sheep, to evaluate a number of serological tests and to discuss some epidemiological aspects of brucellosis, was studied. a total of 2,580 unvaccinated syrian female sheep sera samples were tested for b. melitensis antibodies detection using four serological methods: the rose bengal test (rbt), the serum agglutination test (sat), the complement fixation test (cft) and an indirect enzyme-linked immunosorbent assay (ielisa). in addition, ...201121234678
rpoe1, an extracytoplasmic function sigma factor, is a repressor of the flagellar system in brucella melitensis.the genome of brucella melitensis contains genes coding for the sigma factors rpod, rpon, rpoh1, rpoh2, rpoe1 and rpoe2. previously published data show that b. melitensis is flagellated and that an rpoe1 mutant overexpresses the flagellar protein flge. in this study, we demonstrate that mutation of rpoe1 causes an overexpression of the flagellar genes flif, flge, flic, flaf and flbt, correlating with the production of a longer filament and thereby demonstrating that rpoe1 acts as a flagellar rep ...201121273248
role of flbt in flagellin production in brucella melitensis.it was recently demonstrated that the pathogen brucella melitensis produces a polar sheathed flagellum under the control of the master regulator ftcr. however, the regulatory mechanism controlling the flagellar assembly remains unknown. in this work, we investigate the flagellar hierarchy of b. melitensis as well as the flagellin flic regulation. we show that a mutation in flif or flge (coding for the basal body structure and the hook, respectively) does not affect flic synthesis, suggesting tha ...201121273249
brucellosis acquired by eating imported cheese.pyrexia of unknown origin is an important clinical presentation in both paediatric and adult medicine. we present a case of pyrexia of unknown origin in a 14 year-old boy which turned out to be due to infection with brucella melitensis, despite the patient not having left great britain - an officially brucellosis-free country - in six years. repeated history-taking provided a clue to the diagnosis.201121276115
b. melitensis rough strain b115 is protective against heterologous brucella spp. infections.brucellosis is one of the most serious zoonoses all over the world, with b. melitensis, b. abortus and b. suis being the most pathogenic species for humans. vaccination of domesticated livestock still represents the most efficient way to prevent human infection. however, the available brucella vaccines retain an important residual virulence and induce antibodies interfering with surveillance programs. moreover, each vaccine shows different protective effects versus different brucella species and ...201121300102
[pet-ct scan in a case of suspicion of metastatic dissemination due to the presence of multiple pulmonary nodules]. 201121440334
expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of brucella melitensis m5-90: a potential antigen for differential serodiagnosis of brucellosis in sheep and goats.antibodies produced in animals vaccinated using live attenuated vaccines against brucella spp. are indistinguishable using current conventional serological tests from those produced in infected animals. one potential approach is to develop marker vaccines in which specific genes have been deleted from parental vaccine strains that show good immunogenicity and vaccine efficacy. corresponding methods of detection for antibodies raised by the marker vaccine should also be developed. a specific frag ...201121446957
antimicrobial susceptibilities of brucella isolates from various clinical specimens.brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. in the study we claimed to identify brucella species from clinical samples of patients with active brucellosis from van region of eastern anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-brucella agents and a possible new alternative tigecycline.201121448305
brucellosis presenting as piriformis myositis: a case report. 201121450058
isolation of brucella melitensis from a rb51-vaccinated seronegative goat.the purpose of this study is to determine the etiology of abortions presented in a goat herd declared as free of brucellosis and vaccinated with rb51 located in mexico. the serological diagnosis of brucellosis in 33 animals was performed. the study included three goats that aborted in the last third of gestation and 15 goats that gave birth normally; samples of milk and vaginal exudate were subjected to bacteriological study. all animals were negative for serological diagnosis, and isolation of ...201121455694
bilateral pulmonary nodules due to brucellosis. 201121470756
complete genome sequences of brucella melitensis strains m28 and m5-90, with different virulence backgrounds.brucella melitensis is a gram-negative coccobacillus bacteria belonging to the alphaproteobacteria subclass. it is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. the b. melitensis strain m5-90, a live attenuated vaccine cultured from the b. melitensis virulent strain m28, has been an effective tool to control brucellosis in goats and sheep in china. here we report the complete genome sequences of b. melitensis m28 and m5-90, strai ...201121478357
endovascular repair of aortic rupture due to brucella aortitis.brucellosis is a zoonosis, caused by bacteria belonging to the genus brucella. aortic involvement is a rare complication, often following embolization from infective endocarditis. however, contiguous propagation from vertebral involvement may occur. we report the case of an 81 year old patient abruptly presenting with aortic rupture due to brucella melitensis infection. the diagnosis of aortic rupture was made by ct. the patient underwent urgent endovascular treatment using endoprosthesis deploy ...201121500181
the 16mδvjbr as an ideal live attenuated vaccine candidate for differentiation between brucella vaccination and infection.brucellosis brings great economic burdens for developing countries. live attenuated vaccines are the most efficient means for prevention and control of animal brucellosis. however, the difficulties of differentiating of infection from vaccine immunization, which is essential for eradication programs, limit their applications. therefore, the development of a vaccine that could differentiate infection from immunization will overcome the limitations and get extensive application. vjbr is a quorum s ...201121530111
[direct detection of the brucellosis-causing pathogen--a case report].bacterial zoonoses still represent a serious medical problem. one of the less frequent but severe zoonoses is brucellosis caused by the bacterium brucella melitensis. the presented case report describes relapsing imported brucellosis in a young male. in addition to four serological tests, the diagnosis was confirmed by direct detection of the pathogen in blood culture. the isolate of brucella melitensis was identified using the maldi-tof biotyper method and subsequently also by pcr.201121574131
evaluation of a nanobody phage display library constructed from a brucella-immunised camel.brucella are invasive gram-negative bacteria that multiply and survive within eukaryotic cells causing brucellosis. syrian (and middle east) health and economy sectors are still affected by this disease causing a serious national problem that needs to be solved. here, a strategy was developed to introduce a new generation of binders, known as nanobodies (nbs) in our combat against brucella. these nbs, recombinant single-domain variable fragments derived from camelid heavy-chain antibodies are ve ...201121592585
the whole genome sequences of four brucella strains.brucella melitensis and brucella suis are intracellular pathogens to livestock and humans. here we report four genome sequences, the virulent strain b. melitensis m28-12 and vaccine strains b. melitensis m5, m111 and b. suis s2 that show varied virulence and pathogenicity, which will help to design more effective brucellosis vaccine.201121602346
soluble expression and purification of brucella cell surface protein (bcsp31) of brucella melitensis and preparation of anti-bcsp31 monoclonal antibodies.brucella cell surface protein (bcsp31) is potentially useful for diagnosing brucellosis. we aimed to establish a monoclonal antibody (mab) against brucella melitensis bcsp31 and to investigate its distribution in diagnosis. soluble recombinant bcsp31 was successfully expressed and purified. two mabs (1f1 and 1e5) against b. melitensis bcsp31, effective in detecting both recombinant and cellular proteins, were obtained and characterized. the mabs did not react with escherichia coli, staphylococcu ...201121603860
comparison of different pcr methods for detection of brucella spp. in human blood samples.for detection of brucella species by pcr four dna extraction methods and four targets were compared using pure culture of brucella melitensis and the best conditions were applied in clinical samples. it was found that the magna pure lc method was the most efficient and sensitive method showing a positive pcr reaction with dna extracted from as low as 25 and 100 cfu suspended in one ml blood and one ml water, respectively. detection of brucella spp. by conventional pcr was investigated using four ...201121630571
brucellosis in camels.camels are highly susceptible to brucellosis caused by brucella melitensis and brucella abortus. difficulties can arise in diagnosis of camel brucellosis, especially as this disease provokes only few clinical signs in contrast to its clinical course in cattle. because none of the commonly used serological test can be perceived as a perfect test for brucella diagnosis in camel and most serological tests used for camels have been directly transposed from cattle without adequate validation, an inco ...201121632084
multi-comparative systems biology analysis reveals time-course biosignatures of in vivo bovine pathway responses to b.melitensis, s.enterica typhimurium and m.avium paratuberculosis.to decipher the complexity and improve the understanding of host-pathogen interactions, biologists must adopt new system level approaches in which the hierarchy of biological interactions and dynamics can be studied. this paper presents the application of systems biology for the cross-comparative analysis and interactome modeling of three different infectious agents, leading to the identification of novel, unique and common molecular host responses (biosignatures).201121645321
molecular typing of brucella species isolates from livestock and human.although host specificity has been observed in different species of brucella, crossing the animal host boundary is likely to occur at any time. in this study, bruce ladder pcr and abortus-melitensis-ovis-suis (amos) pcr assays were used to characterize 47 brucella isolates from indian origin in order to know exact species for understanding epidemiology of brucellosis. out of them, 28, 14, and 5 isolates were found to be brucella abortus, brucella melitensis, and brucella suis, respectively. furt ...201121647774
enhancing the role of veterinary vaccines reducing zoonotic diseases of humans: linking systems biology with vaccine development.the aim of research on infectious diseases is their prevention, and brucellosis and salmonellosis as such are classic examples of worldwide zoonoses for application of a systems biology approach for enhanced rational vaccine development. when used optimally, vaccines prevent disease manifestations, reduce transmission of disease, decrease the need for pharmaceutical intervention, and improve the health and welfare of animals, as well as indirectly protecting against zoonotic diseases of people. ...201121651944
infection of cattle in kenya with brucella abortus biovar 3 and brucella melitensis biovar 1 genotypes.brucella melitensis biovar 1 was isolated from bovine milk samples from a herd in central kenya, and brucella abortus biovar 3 was isolated from aborted fetus materials and vaginal discharge fluids from cattle in central and eastern provinces of kenya. all infections including those with b. melitensis were in cattle with reproductive problems kept in mixed herds indicating that cross infection occurs from small ruminants. multiple-locus variable-number tandem repeat analysis genotyping revealed ...201121660647
transcriptional profile of the intracellular pathogen brucella melitensis following hela cells infection.brucella spp. infect hosts primarily by adhering and penetrating mucosal surfaces; however the initial molecular phenomena of this host:pathogen interaction remain poorly understood. using cdna microarray analysis, we characterized the transcriptional profile of the intracellular pathogen brucella melitensis at 4h (adaptational period) and 12h (replicative phase) following hela cells infection. the intracellular pathogen transcriptome was determined using initially enriched and then amplified b. ...201121798337
mlva genotyping of human brucella isolates from turkey.a multiple loci variable-number tandem-repeat analysis (mlva) was applied to investigate the epidemiological relationship and genetic diversity among 162 human brucella isolates collected from all geographic regions of turkey in an 8-year period (2001-2008). the isolates were genotyped by using mlva-16(upsud) including eight minisatellite (panel 1) and eight microsatellite (panel 2 subdivided in 2a and 2b) markers. panels 1 and 2a distinguish 14 genotypes; two of these represented 85% of the str ...201121795514
[genetic characterization of the brucella melitensis isolates from mongolia, russia, and azerbaijan].the goal of this work was to provide comparative genetic characterization of the human and animal brucella melitensis isolates from mongolia, russia and azerbaijan using current molecular-genetic typing methods.201121786630
cutaneous manifestations in brucellosis.brucellosis is a common worldwide zoonotic disease. cutaneous manifestations are not specific and affect 1-14% of patients with brucellosis. here, we describe 49-year-old female with fever and a diffuse maculopapular rash due to brucella melitensis infection. histopathology of skin biopsy revealed leukocytoclastic vasculitis; positive blood cultures for b. melitensis established the diagnosis of brucellosis. we provide a review of the relevant literature.201121772606
protective live oral brucellosis vaccines stimulate th1 and th17 cell responses.zoonotic transmission of brucellosis often results from exposure to brucella-infected livestock, feral animals, or wildlife, or frequently via consumption of unpasteurized milk products or raw meat. since natural infection of humans often occurs by the oral route, mucosal vaccination may offer a means to confer protection for both mucosal and systemic tissues. significant efforts have focused on developing a live brucellosis vaccine, and deletion of znua gene involved in zinc transport has been ...201121768283
Refresh
firstprev
12345678910...
nextlast
Displaying items 401 - 500 of 2066