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[the distribution of intestinal parasites in students of the mustafa kemal university school of health.].intestinal parasites are an important national health problem in our country as in the rest of the world. in our study, the prevalence of intestinal parasites in female students (aged from 16-18 years) in the mustafa kemal university school of health was investigated. fecal samples and cellophane tape preparations were used for diagnosis. for this reason 142 fecal samples and 136 cellophane tape preparations were examined. one or more parasites were found in 65 (45.77%). fecal samples. the preva ...200517124683
[experimental infection of mice with blastocystis hominis].to seek a better pathway and proper number of parasites for blastocystis hominis (b.h) infection in normal and immunocompromised icr mice.200516566218
[emphasizing of blastocystis hominis on culture media].the aim of our study was to determine the frequency of blastocystis hominis in the stool specimens sent to the parasitological laboratory of the mureş county hospital between october 2003 and may 2004. the 124 inoculated specimens showed positive values in 41.93%. after an incubation in anaerobic conditions at 37 degrees c for 24, 72 and 96 hours in liquid and biphasic media, the last proved to be better (69,24%) at an incubation time of 72 hours.200416752753
chronic urticaria and blastocystis hominis infection: a case report.we report a case of a 45 year old woman which fulfilled the criteria of chronic urticaria (remitting and relapsing bouts of erythematous and pruriginuos lesions without angioedema, lasted four months). cutaneous manifestations were not related to a specific inducing factor, had no benefit from antihystamine and steroid drugs and were associated sometimes with mild gastroentric disorders. patient was submitted to extensive clinical, laboratory and intrumental investigations which permit to exclud ...200415368795
epidemiological survey of giardia spp. and blastocystis hominis in an argentinian rural community.the aim of this study was to relate personal data, socio-cultural and environmental characteristics, and the presence of symptoms/signs with the frequencies of giardia spp. and blastocystis hominis among a rural population in buenos aires province, argentina. of the surveyed population (350), 3.7% were infected with only giardia spp. or 22.9% with b. hominis, and 2.3% were infected with both protozoa. the frequency of infection according to sex; 6.1% of males were infected and 1.6% of females by ...200415381860
isolation and characterization of the mitochondrion-like organelle from blastocystis hominis.blastocystis hominis in an unusual protozoan parasite of the human intestinal tract. previous studies have described the presence of mitochondrial-like structures despite the anaerobic nature of the organism. in this study, we describe a simple and rapid technique to isolate and characterize mitochondrion-like organelles (mlo) from b. hominis. the parasite was disrupted using glass beads and the mlo were collected and purified using a sucrose gradient. negative staining and transmission electron ...200415177908
[digestive parasitism in sousse area. results of a 16 years retrospective study].to report the prevalence and type of intestinal parasites together with their kinetics in sousse region. our retrospective study concern data from the examination of 56,268 stool samples and 7552 tape tests, performed in the laboratory of parasitology of f. hached sousse hospital from 1st january 1987 up to 31 december 2002. we observed an overall parasite prevalence of 31.5%. most common parasites were protozoa, giardia lamblia, dientamoeba fragilis and blastocystis hominis being the most frequ ...200415453028
blastocystis hominis as a cause of hypoalbuminemia and anasarca.the protozoan blastocystis hominis has been considered nonpathogenic, but this classification has come under scrutiny in light of reports in the medical literature indicating it could be the cause of intestinal disorders and, in one case, hypoalbuminemia. reported here is a severe case of infection with b. hominis that caused acute gastroenteritis with prolonged diarrhea, hypoalbuminemia and anasarca. the diagnosis was based on the parasitological finding, since no other pathological evidence wa ...200415112065
infection status of intestinal parasites in children living in residential institutions in metro manila, the philippines.a small scale survey was performed to know the infection status of intestinal parasite in children of the residential institutions and street communities in metro manila, philippines. a total of 284 stool samples from 11 institutions and 3 street communities was examined by the formalin-ether concentration method. the scotch tape anal swab was adapted to 121 children to investigate the infection status of enterovius vermicularis. it was found out that 62.0% of the children examined were positive ...200415181346
evidence of waterborne transmission of blastocystis hominis.a cross-sectional study was performed in february 2001 to evaluate the prevalence and risk factors of blastocystis hominis infection in army personnel who resided in an army base in chonburi, thailand. a total of 904 army personnel were enrolled in this study. short-term in vitro cultivation was used to detect b. hominis in stool samples. in this population, b. hominis was the parasite most frequently found, and was identified in 334 of 904 stool specimens (36.9%). a significant association betw ...200415211009
protozoal infections in the developmentally disabled: often overlooked cause of rectal digging and fecal smearing. 200415226486
the usefulness of short-term in vitro cultivation for the detection and molecular study of blastocystis hominis in stool specimens.when in vitro cultivation was used as the 'gold standard' for the detection of blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. in vitro cultivation also enhanced pcr amplification for the detection of b. hominis in stool specimens. our data show the usefulness of in vitro cultivation for the detection and molecular study of b. hominis in stool specimens.200415243800
in vitro susceptibility of blastocystis hominis isolated from patients with irritable bowel syndrome.this study aims to determine the growth pattern and in vitro susceptibility of clinical isolates of blastocystis hominis to different concentrations of metronidazole, furazolidone and ciprofloxacin. stool specimens from 25 consecutive patients with irritable bowel syndrome presenting to the gastroenterology department of aga khan university hospital between january and may 2003 are examined by microscopy and cultured for b. hominis. drug susceptibility assays are performed for metronidazole, fur ...200415250669
enteric infection in relapse of inflammatory bowel disease: importance of microbiological examination of stool.previous reports have suggested that diarrhoeal relapses of inflammatory bowel disease (ibd) may be associated or confused with enteric infection, and that treatment of such infections with appropriate antibiotics may be beneficial. to re-evaluate the suggestion that enteric infection is rare and microbiological testing of stool not routinely necessary in patients presenting with relapse of ibd, we have reviewed the incidence of concurrent infection in patients presenting in relapse over a recen ...200415256979
point prevalence of cryptosporidium, cyclospora, and isospora infections in patients being evaluated for diarrhea.from march to september 2001, 315 specimens from "nonrepeat" patients that were submitted for ova and parasite examination were stained using the kinyoun modified acid-fast stain to detect the intestinal coccidians. four patients (1.3%) were infected with coccidians, 2 with cryptosporidium parvum and 2 with cyclospora cayetanensis. no infections with isospora belli were detected. in comparison, 15 patients (4.8%) had infections with one or more intestinal parasites detected by routine trichrome ...200415272527
[investigation of intestinal parasites in aids patients].in this study, enteric parasites were investigated in the stool samples of 38 aids patients (23 with chronic diarrhea and 15 without diarrhea) prospectively. at least three stool samples from each patient were investigated microscopically for ova or trophozoites. the samples were concentrated with formol-ether method and wet preparations stained with lugol were examined. in addition, the concentrated samples were stained with modified asid-fast (kinyoun's), rhodamine-auramine, modified trichrom ...200415293911
fecal-oral transmission of the cyst form of blastocystis hominis in rats.the infectivity of two blastocystis hominis strains, rn94-9 and nih:1295:1, was examined in 3-week-old spf wistar rats. the nih:1295:1 strain, originally isolated from a guinea pig, was only able to infect rats via intracecal inoculation of the cultured organisms, while the rn94-9 strain, originally isolated from a laboratory rat, was able to infect rats by oral inoculation of the cultures due to the presence of a cystic form in the in vitro culture. since many cysts were discharged in the feces ...200415480786
a survey of blastocystis infection in anuran and urodele amphibians.blastocystis infection in amphibians was surveyed in three species of anuran and one species of urodele amphibians captured at two distinct locations in japan. all three species of frogs were highly infected with blastocystis, while 69 individual urodele newts, cynopus pyrrhogaster, were negative for infection. eleven blastocystis isolates (47.8%) were recovered from 23 rana nigromaculata leopard frogs. twenty-three (92%) of 25 rana catesbeiana bullfrogs and all (100%) of 24 bufo japonicus japon ...200415177714
comparison of methods for detecting blastocystis hominis.in order to determine the comparative sensitivity of two methods of detecting blastocystis hominis and to investigate the seasonality of infection with this enteric protozoan parasite, the present study was conducted. in each of two 3-month periods representing winter, spring (february-april) and summer (july-september), 500 routine stool submissions were examined for b. hominis using microscopy following either formol-ether concentration or in vitro culture using jones' medium. the organism was ...200415168139
pcr-based identification of zoonotic isolates of blastocystis from mammals and birds.the genotype of blastocystis isolated from humans and animals is highly polymorphic. therefore, it is important to compare the genotypes of blastocystis isolates from humans and animals to determine the zoonotic potential of animal isolates. pcr-based genotype classification using known sequence-tagged site (sts) primers allows identification of zoonotic isolates of animal origin. to this end, 51 isolates from monkeys, cattle, pigs, chickens, quails and pheasants were subjected to genotype analy ...200415133074
molecular and phylogenetic analysis of blastocystis isolates from various hosts.blastocystis hominis is a protozoan parasite found in humans. b. hominis-like organisms have been found in a variety of animals, but have been called blastocystis sp. because the isolates from animals were indistinguishable from b. hominis morphologically. recent molecular studies show that some isolates from animals have genetic similarity with b. hominis. however, it has been unclear whether the isolates from animals have zoonotic potential or not. in the present study, the ssurdna of 19 blast ...200415041098
irritable bowel syndrome: in search of an etiology: role of blastocystis hominis.this study was designed to examine stool specimens of irritable bowel syndrome (ibs) patients for blastocystis hominis, a common intestinal parasite. one hundred fifty patients were enrolled, 95 ibs cases and 55 controls. these patients provided a medical history, and underwent physical and laboratory evaluations that included stool microscopy and culture for b. hominis and colonoscopy. the 95 cases (51 males and 44 females) had a mean +/- sd age of 37.8 +/- 13.2 years. stool microscopy was posi ...200415100450
metronidazole induces programmed cell death in the protozoan parasite blastocystis hominis.previous studies by the authors have shown that the protozoan parasite blastocystis hominis succumbed to a cytotoxic monoclonal antibody with a number of cellular and biochemical features characteristic of apoptosis in higher eukaryotes. the present study reports that apoptosis-like features are also observed in growing cultures of axenic b. hominis upon exposure to metronidazole, a drug commonly used for the treatment of blastocystosis. upon treatment with the drug, b. hominis cells displayed k ...200414702395
cryopreservation of protozoan parasites.conventional methods for the propagation and preservation of parasites in vivo or in vitro have some limitations, including the need for labor, initial isolation and loss of strains, bacterial, and fungal contamination, and changes in the original biological and metabolic characteristics. all these disadvantages are considerably reduced by cryopreservation. in this study, we examined the effects of various freezing conditions on the survival of several protozoan parasites after cryopreservation. ...200414969677
prevalence of enteric parasites in pet macaques in sulawesi, indonesia.on the indonesian island of sulawesi, nonhuman primate pets come into frequent contact with humans, presenting the possibility of zoonotic and anthropozoonotic disease transmission. we collected fecal samples from 88 pet macaques representing six of the seven macaque species currently recognized as endemic to sulawesi (macaca nigra, m. nigrescens, m. hecki, m. tonkeana, m. maura, and m. ochreata) as well as two non-endemic species (m. fascicularis and m. nemestrina) in order to determine the pre ...200414983465
changing patterns in intestinal parasitic infections among southeast asian laborers in taiwan.in order to determine whether there are changing patterns in intestinal parasitic infections among foreign laborers in north taiwan, one fecal specimen was obtained from each of 1,569 laborers and examined by the formalin-ethyl acetate sedimentation technique. the prevalence of intestinal parasitic infections was 8.2%. females (11.7%) had a significantly higher prevalence than males (6.9%), although there was no significant difference between filipinos (7.7%), thais (8.3%) and indonesians (12.5% ...200414586642
caspase-3-like protease influences but is not essential for dna fragmentation in blastocystis undergoing apoptosis.blastocystis hominis undergo apoptosis after treatment with a cytotoxic monoclonal antibody (mab), 1d5, by mechanisms that are not fully understood, although our previous study demonstrated that caspase-3-like protease activity is involved. to elucidate the mechanism of mab 1d5-induced apoptosis, we inhibited blastocystis caspase-3-like protease to investigate if there would be a concomitant decrease in in situ dna fragmentation. however, mab 1d5-induced apoptosis, evidenced by dna fragmentation ...200415540464
surveillance for enteric parasites among u.s. military personnel and civilian staff on joint task force base-bravo in soto cano, honduras and the local population in comayagua and la paz, honduras.a study was conducted in honduras to address whether military personnel assigned to joint task force-bravo in soto cano, honduras, routinely acquire parasite infections, and the results were compared with those collected from civilian base workers and the general local population in the nearby towns of comayagua and la paz. results from this study report 21 species of enteric parasites among hondurans living in comayagua and la paz, 13 species among local hondurans working as base civilian perso ...200415605940
prevalence of intestinal parasitic pathogens among hiv-positive individuals in iran.parasites are important enteric pathogens among patients with human immunodeficiency virus (hiv) infection. there have been very few reports on the prevalence of intestinal parasites among such patients in iran. to determine the prevalence of intestinal parasites among hiv-positive individuals, we collected single stool samples and analyzed them for detection of various intestinal parasites from 206 hiv-positive individuals with different immune status visited in different medical centers in ira ...200415623953
blastocystis hominis infection in irritable bowel syndrome patients.irritable bowel syndrome (ibs) is a functional bowel disorder in which abdominal pain is associated with a defect or a change in bowel habits. subtle inflammation, especially after infectious enteritis, has been sometimes suspected as one mechanism of pathogenesis. this research was performed (1) to evaluate the prevalence of parasitic infections and (2) the possible association of ibs and parasitic infections. fifty-nine ibs patients were recruited using symptom-based criteria (rome criteria ii ...200415689092
[blastocystosis is human protozoosis]. 200415689141
intestinal parasites in children from three west texas border communities. 200415724569
[blastocystosis: a high proportion of cases found in schoolchildren of são paulo, state of são paulo, brazil].as a part of medical assistance activities, parasitological examination of fecal samples from 227 school children from a public institution of são paulo (sp) revealed a rather high proportion of results positive for blastocystis hominis. other protozoan and worm species were markedly scarcer, a peculiar situation according to our judgement. it is acknowledged that blastocystosis is still largely an indefinite and controversial subject, which deserves adequate analysis to avoid drawbacks in the s ...200415334272
serum zinc and magnesium levels in patients with blastocystosis.the aim of the study was to investigate the total content of the essential elements of zinc and magnesium levels in patients infected with blastocystis hominis. zinc and magnesium concentrations were measured in 52 patients who were positive for the intestinal parasite blastocystis hominis. scores were obtained for the positives and their age- and sex-matched 60 blastocystis hominis-negative healthy controls. for comparison of two groups of continuous variables, the independent samples t-test wa ...200415051897
cutaneous lesions in blastocystis hominis infection. 200415339085
microorganisms and parasites in chronic infective diarrhea.to reveal the pattern of microorganisms in chronic infective diarrhea cases.200415931720
polymerase chain reaction-based genotype classification among human blastocystis hominis populations isolated from different countries.since the genotype of human blastocystis hominis isolates is highly polymorphic, pcr-based genotype classification using known sequenced-tagged site (sts) primers would allow the identification or classification of different genotypes. five populations of human b. hominis isolates obtained from japan, pakistan, bangladesh, germany, and thailand were subjected to genotype analysis by using seven kinds of sts primers. ninety-nine out of 102 isolates were identified as one of the known genotypes, w ...200414598169
zoonotic intestinal parasites in papio anubis (baboon) and cercopithecus aethiops (vervet) from four localities in ethiopia.a total of 59 faecal samples from ranging papio anubis (baboons) and another 41 from cercopithecus aethiops (vervet) from the rift valley areas of ethiopia were microscopically examined to determine the prevalence and species of major gastro-intestinal parasites of zoonotic importance. faecal smears were prepared from fresh faecal samples, stained using modified ziehl-neelsen method and microscopically examined. about 3 gm of the dropping was also preserved separately in clean and properly label ...200415099809
enteropathogens associated with cases of gastroenteritis in a rural population in jordan.stool specimens were collected from 180 patients belonging to a population of recently settled bedouins in jordan who presented with acute or persistent diarrhoea and other symptoms, and from 100 non-diarrhoeal controls. all samples were examined for parasites and bacterial pathogens by culture and pcr. bacterial isolates were tested for their susceptibility to common antimicrobial agents. pathogens and potential enteropathogens were identified from 140 (77.8%) of the patients, with more than on ...200415214876
[prevalence of blastocystis hominis among food handlers from caroni municipality, bolivar state, venezuela].a cross-sectional survey was conducted to determine the prevalence of blastocystis hominis infection in a random sample of apparently healthy food handlers. a total of 415 individuals attending the manoa urban outpatient clinic (caroní municipality, bolívar state, venezuela) in the adult hygiene program and who requested health certification to work as food handlers were studied. stool samples obtained by spontaneous evacuation were examined by direct microscopy and the willis concentration meth ...200414999338
[blastocystosis: controversy and indefinedness].blastocystosis is the infection caused by blastocystis hominis. it is associated with frequent and unquestionably very important controversy and lack of definition, above all due to its implications for general assistance and medical care. in that connection, there is considerable disagreement on the subject of the pathogenicity of this protozoan, which should be categorically defined. other aspects besides the above, require clarification through results from well conducted studies aiming at at ...200312937731
[prevalence of microsporidia and other intestinal parasites in patients with hiv infection, bogota, 2001].opportunistic intestinal parasites are a common cause of diarrhea in hiv-infected patients. to determine the prevalence of microsporidia and other opportunistic parasites infecting hiv patients in bogotá, colombia, 115 patients were examined for these infections during the year 2001. the institution and the sample percent from each are as follows: santa clara hospital, 33.0%; san pedro claver, 20.0%; simón bolívar hospital, 14.8%; san josé hospital, 13.9%; central de la policía hospital, 6.1%; c ...200314582330
blastocystis isolates from a pig and a horse are closely related to blastocystis hominis.blastocystis has a widespread distribution in a variety of animals, which is a potential source of infection for humans. however, the contribution of zoonotic transmission remains unclear due to the absence of molecular proof of these organisms being identical to those found in humans. we report herein the similar subgroup of blastocystis isolates from humans, pigs, and a horse using a restriction fragment length polymorphism (rflp) analysis of partial small-subunit ribosomal dna (ssu rdna). add ...200312624017
molecular characterization of blastocystis isolates from primates.twelve blastocystis isolates from primates were analyzed genetically by polymerase chain reaction (pcr) using diagnostic primers and pcr-restriction fragment length polymorphism (rflp) of ssurdna. two distinct genotypes, subtype 1 and a variant of subtype 1, were detected in two and six of the 12 isolates, respectively. the rflp profiles of the isolates designated as subtype 1 were identical to the profile of ribodeme 1. the rflp profiles of the six isolates designated as variants of subtype 1 w ...200312719144
prevalence of intestinal parasites among individuals with allergic skin diseases.the prevalence of intestinal protozoans and helminths in stool samples of individuals with allergic cutaneous symptoms was evaluated to study a possible link between parasites and allergy. altogether, 218 patients who had chronic urticaria, atopic dermatitis, or pruritus of unknown origin were included in the study. standard laboratory tests for the detection of allergic etiology were performed for all patients. the presence of intestinal parasites was investigated using microscopy, immunofluore ...200312880246
epidemiology and control of intestinal parasites with nitazoxanide in children in mexico.the purpose of this study was to evaluate the efficacy and the tolerance of nitazoxanide in children as a single broad-spectrum antiparasitic agent in the treatment of mixed parasite infections with both intestinal protozoa and helminths. two hundred seventy-two children (age range = 2-14 years) participated in this study. we systematically surveyed every household head using questionnaires designed to obtain information about household socioeconomic status and hygiene. parasitic infections were ...200312875284
[study on morphology of blastocystis hominis in culture and from diarrhea patients].to study morphology of different stage blastocystis hominis (b. h) for establishing a base in the research of life cycle and pathogenicity of b. h and providing information for clinical laboratory.200312884629
[influence of the protozoa blastocystis hominis on hepatic function]. 200312886585
[blastocystosis: nitazoxanide as a new therapeutic option].we report a case of a patient with aids and severe immunosuppression who developed diarrhea due to a vacuolar stage of blastocystis hominis. the patient was treated with nitazoxanide, a 5-nitrothiazole broad-spectrum antiparasitic agent. we obtained complete remission of symptoms and eradication of the parasite. nitazoxanide is a new treatment option for blastocystosis in aids patients. further studies to test its efficacy are needed.200312908045
intestinal parasitic infestations among children in an orphanage in pathum thani province.infection caused by intestinal parasites is still a common health problem especially in children from developing countries. orphans are a group of underprivileged population in society. to evaluate the intestinal parasitic infections in children in an orphanage in pathum thani province, thailand, stool samples were collected during a cross-sectional study in april 2001. examination for intestinal parasites were performed by using simple smear, formalin-ether concentration, boeck and drbohlav's l ...200312929999
zoonotic genotypes of blastocystis hominis detected in cattle and pigs by pcr with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal rna gene.twenty two blastocystis isolates from cattle and pigs were genotyped by pcr using diagnostic primers, and the homology among isolates was then confirmed by restriction fragment length polymorphism (rflp) analysis of the small subunit ribosomal rna gene. two distinct genotypes, subtypes 1 and 3, were detected in one and two of the ten isolates from cattle, and three and one of the 12 isolates from pigs, respectively. the rflp profiles of all isolates designated as subtype 1 and subtype 3 were ide ...200312756546
secretory and humoral antibody responses to blastocystis hominis in symptomatic and asymptomatic human infections.the study included 3 groups of individuals, in the first 2 groups they had positive stool microscopic examinations only for b. hominis indicating blastocystosis, with and without gastrointestinal symptoms, respectively, while the last group included apparently healthy individuals with no parasites in stool. stool and serum samples of these individuals were subjected to detection of anti-b. hominis fecal and serum iga and serum igg antibodies by indirect elisa, and detection of b. hominis fecal a ...200312739797
rotavirus type a and other enteric pathogens in stool samples from children with acute diarrhea on the colombian northern coast.the present study, conducted from march 1998 to july 2000, determined the etiology of acute diarrhea in 253 young children and infants from cartagena and sincelejo, colombia. in 253 stool samples, the following enteric pathogens were recovered: rotavirus type a (36.6%) as the major agent, salmonella spp (9.0%), shigella spp (8.0%), enteric pathogenic escherichia coli (6.0%), enteric hemorragic esc. coli (2.8%), providencia alcalifaciens (2.8%), aeromonas hydrophila (2.0%), yersinia enterocolitic ...200312687410
intestinal parasitic infection and associated symptoms in children attending day care centres in trujillo, venezuela.to examine the presence of intestinal protozoan and helminth infections and their association with clinical signs and symptoms in children in trujillo, venezuela.200312667154
clinical characteristics and endoscopic findings associated with blastocystis hominis in healthy adults.ninety-nine individuals with stools positive for blastocystis hominis but negative for other parasites were identified from medical records of healthy adults who had received a physical examination at taipei veterans general hospital from november 2000 to october 2002. the medical records of these 99 positive cases and 193 randomly selected controls, matched for age, sex, and date of examination, were retrospectively reviewed. the pathogenicity of b. hominis could not be demonstrated due to a la ...200313677378
molecular characterization of blastocystis isolates from birds by pcr with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal rna gene.seven blastocystis isolates from birds were genotyped by polymerase chain reaction (pcr) using diagnostic primers, and the homology among isolates was then confirmed by restriction fragment length polymorphism (rflp) analysis of the small subunit ribosomal rna gene. a zoonotic genotype, subtype 4, was detected in three isolates by pcr, but the rflp profiles of these isolates differed from those of the subtype 4 reported previously from a human. none of the other four isolates were amplified with ...200312632154
a placebo-controlled treatment trial of blastocystis hominis infection with metronidazole.blastocystis hominis, previously considered a harmless yeast, is now classified as a protozoan inhabiting the human intestinal tract. the pathogenicity of b. hominis remains controversial and is currently the subject of extensive debate.1- 5 as a result of the uncertainty surrounding the pathogenic role of b. hominis, large-scale treatment trials of b. hominis infection have so far been lacking. in spite of this, several drugs have been reported to be active against the parasite.6-8 the present ...200312650658
hematological effects of blastocystis hominis infection in male foreign workers in taiwan.blastocystis hominis found in stool specimens has been the most frequently identified parasite among foreign workers from southeast asia in taiwan since 1992. the prevalence of b. hominis was 14.1% in this study. in their quarantine physical examinations, 121 male thai workers were examined hematologically and screened for stool parasites using the merthiolate-iodine-formaldehyde concentration method. hematological values were compared in workers with and without a b. hominis infection. multiple ...200312743803
blastocystis hominis among food vendors in xochimilco markets.blastocystis hominis is a pathogenic protozoon that lives in the human bowel and causes diarrhea: the mode of transmission is a passive one, through the ingestion of stool-contaminated water or foods that contain infective forms of the parasite. the purpose of this study is to report the prevalence of blastocystis hominis among food vendors in the markets within the xochimilco jurisdiction, mexico city, mexico. a cross-sectional study was conducted in which food vendors answered an epidemiologic ...200317061516
polypeptides associated with in vitro cyst formation of blastocystis hominis.the objective of this study was to characterize the polypeptides associated with cysts of blastocystis hominis. this form is believed to be infective and plays a role in parasite resistance to anti-b. hominis drugs currently used for treatment of blastocystis associated diarrhea. cysts were induced through in vitro culture of the parasite in complete medium supplemented with bacterial extract with trypticase, metronidazole or doxycycline. sds-page analysis showed almost similar polypeptide patte ...200315115079
intestinal parasites of the pacific.information about intestinal parasites in hawaii and the pacific is not current. we reviewed reports on fecal samples obtained from two laboratories and found recovery rates of 9.3% in hawaii, 14.2% in saipan, 18% in rota and 9.5% in guam. the most frequently identified parasites were blastocystis hominis (7.6%), giardia lamblia (1.2%), and entamoeba coli (0.7%). although the incidence and types of organisms have changed with time, physicians in hawaii should continue looking for intestinal para ...200314631593
[human blastocystosis: prospective study symptomatology and associated epidemiological factors].an attempt has been made to contribute to the understanding of the symptoms and factors associated with the blastocystis hominis infection, as seen in persons seeking outside consultation from the dermatological and transmissible diseases department (dtdd) at the c.h.n.h. this is a case-control study carried out in people between the ages of 5 and 80 in a period from january to march 1999. the cases tested positive in parasitological tests for blastocystis hominis and were absent of other entero ...200312768212
multiple reproductive processes in blastocystis hominis. 200312855375
blastocystis hominis--an emerging cause of diarrhoea in renal transplant recipients.blastocystis hominis is an intestinal protozoan that is emerging as an important cause of diarrhea in the immunosuppressed population. we report two cases of diarrhea caused by this organism in renal transplant recipients. the infection was diagnosed promptly by careful stool examination and treated successfully with metronidazole. these case report highlights the fact that unusual parasites like blastocystis hominis should be looked for and treated in cases of diarrhea occurring in renal transp ...200314621046
[treatment of blastocystis hominis protozoosis with baitouweng decoction: a report of two cases]. 200315339549
a cross-sectional study of intestinal parasitic infections in a rural district of west china.parasitic infections are widespread in rural areas of west china. the remote and humid environment, traditional ways of life, contaminated potable water and limited health services all contribute to the transmission and persistence of fecal parasites.200318159450
a spectrum of pathogenic and non-pathogenic intestinal parasites in pre-employment medical check-up for workers and their families.stool analysis plays an important role in pre-employment tests for the screening of intestinal parasites in new workers.200323011980
incidence of blastocystis hominis in faecal samples submitted for routine microbiological analysis.over a one-year period, 1390 faecal samples were submitted to aberystwyth public health laboratory for routine microbiological examination. all were stained using a commercial trichrome method. blastocystis hominis was detected in 96 (6.9%), making it the most common parasite found in the study. of the b. hominis-positive specimens, 73% were missed on direct microscopy. molecular typing of b. hominis has revealed extensive genetic diversity in morphologically identical strains and thus detection ...200228700853
intestinal parasites, including cryptosporidium species, in iraqi patients with sickle-cell anaemia.stool samples were obtained from individuals admitted to three hospitals in basra during november 1997-may 1998. of 40 patients with sickle-cell anaemia, 25 (62.5%) had parasitic infections. in the apparently healthy comparison group, 26 of 175 individuals (14.8%) had intestinal parasitic infections, a statistically significant difference. the most common intestinal parasites isolated in the sickle-cell patients were blastocystis hominis (36%) and giardia lamblia (28%). the isolation rate of cry ...200215339123
[50-year-old patient returning from hawaii with changed stool habits. blastocystis hominis]. 200212085551
a survey of blastocystis sp. in livestock, pets, and zoo animals in japan.the prevalence of blastocystis sp. was examined in fecal samples collected from cattle, pigs, dogs, and a variety of zoo animals (primates, carnivores, herbivores, pheasants, and ducks) by direct observation of fresh fecal suspensions or cultured materials, using light microscopy. the cattle and pigs were randomly sampled from 11 and 12 commercial farms, respectively, located in the western region of japan. the dog material used in this study was obtained from pets housed in an animal shelter in ...200212062509
recent advances in blastocystis hominis research: hot spots in terra incognita.despite being discovered more than 80 years ago, progress in blastocystis research has been gradual and challenging, due to the small number of laboratories currently working on this protozoan parasite. to date, the morphology of blastocystis hominis has been extensively studied by light and electron microscopy but all other aspects of its biology remain little explored areas. however, the availability of numerous and varied molecular tools and their application to the study of blastocystis has ...200212062550
[blastocystis hominis and blastocystosis (zierdt-garavelli disease)]. 200212154808
[infections and diseases after travelling].with intensifying international travel numbers of travel associated infections and diseases will increase. systematic studies on infections and diseases with regard to the travel destination in tropical and subtropical areas are scarce in germany.200212192633
protozoon infections and intestinal permeability.intestinal permeability (ip) studies using some macromolecules have been assumed to demonstrate the intactness of intestinal mucosa. the aim of the present study is to determine the changes in ip among patients with protozoan infections. thirty nine patients with protozoan infections and ten healthy controls were enrolled in the study. protozoa were diagnosed by native-lugol, richie and trichrome staining of faeces. ip was evaluated by diethyl triamine penta acetic acid labeled with 99m techneti ...200211755426
enteropathogens associated with acute diarrhea in community and hospital patients in jakarta, indonesia.the prevalence of bacteria, parasite and viral pathogens in 3875 patients with diarrhea in community and hospital settings from march 1997 through august 1999 in jakarta, indonesia was determined using routine bacteriology and molecular assay techniques. bacterial pathogens isolated from hospital patients were, in decreasing frequency, vibrio cholerae o1, shigella flexneri, salmonella spp. and campylobacter jejuni, while s. flexneri, v. cholerae o1, salmonella spp. and c. jejuni were isolated fr ...200212381465
phylogenetic position of blastocystis hominis and of stramenopiles inferred from multiple molecular sequence data.blastocystis hominis, a parasite of the human intestine, has recently been positioned within stramenopiles by the small subunit rrna phylogeny. to further confirm its phylogenetic position using multiple molecular sequence data, we determined the nucleotide sequences putatively encoding small subunit ribosomal rna, cytosolic-type 70-kda heat shock protein, translation elongation factor 2, and the non-catalytic 'b' subunit of vacuolar atpase of b. hominis (he87-1 strain). moreover, we determined ...200211908898
[blastocytosis. an italian story]. 200212532568
methods for cultivation of luminal parasitic protists of clinical importance.cultivation of luminal protistan parasites has a long history. in this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: entamoeba histolytica, giardia intestinalis, trichomonas vaginalis, dientamoeba fragilis, blastocystis hominis, and balantidium coli. while cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that ...200212097242
blastocystis hominis and blastocystosis (zierdt-garavelli disease). 200211975117
hypersensitivity to non-steroidal anti-inflammatory drugs and chronic urticaria cured by treatment of blastocystis hominis infection. 200212072100
intestinal parasitic infections in bekasi district, west java, indonesia and a comparison of the infection rates determined by different techniques for fecal examination.this study was undertaken to determine the current status of intestinal parasitic infections among schoolchildren in west java, indonesia, and to compare the infection rates obtained by three different methods of fecal examination. a total of 285 fecal samples were collected from 131 males and 154 females at a junior high school. samples were brought to the department of parasitology, faculty of medicine, university of indonesia, and were examined for parasites by the kato-katz thick smear metho ...200212693577
incidence of blastocystis hominis in faecal samples submitted for routine microbiological analysis.over a one-year period, 1390 faecal samples were submitted to aberystwyth public health laboratory for routine microbiological examination. all were stained using a commercial trichrome method. blastocystis hominis was detected in 96 (6.9%), making it the most common parasite found in the study. of the b. hominis-positive specimens, 73% were missed on direct microscopy. molecular typing of b. hominis has revealed extensive genetic diversity in morphologically identical strains and thus detection ...200212371057
in vitro effect of nitazoxanide against entamoeba histolytica, giardia intestinalis and trichomonas vaginalis trophozoites.nitazoxanide, a 5-nitrothiazolyl derivative, is effective in the treatment of a broad range of parasitic infections. in vitro, it is active against several protozoa, including cryptosporidium parvum, blastocystis hominis, and giardia intestinalis. the objective of this study was to determine the in vitro effect of nitazoxanide on the growth and morphology of three anaerobic protozoa (entamoeba histolytica, giardia intestinalis, and trichomonas vaginalis) and to compare these effects with those o ...200212120985
in-vitro cultivation: a sensitive method for detecting blastocystis hominis.currently, the detection of human infection with blastocystis hominis is usually based on the examination under a light microscope of faecal samples, either directly, as 'simple smears', or after some form of concentration. whether short-term, in-vitro cultivation would increase the sensitivity of such detection remains a matter of controversy. over 900 fresh stool specimens, from soldiers in the royal thai army, were each checked for the parasite using three methods: simple smears; formalin-eth ...200212625935
prevalence of blastocystis hominis carriage in thai army personnel based in chonburi, thailand.blastocystis hominis is one of the most common intestinal parasites found in humans. the prevalence of b. hominis carriage in personnel who worked in the first army support command, chonburi, thailand, was determined. forty-four percent of 616 stool samples were positive for b. hominis using both simple smear and concentration (formalin/ethyl acetate) techniques. the usefulness of the concentration technique in the diagnosis of b. hominis has been confirmed. of 272 positive specimens, 75% were d ...200212188234
[utility of studying feces for the diagnosis and management of infants and preschool children with acute diarrhea].to analyze the results of a stool work-up protocol in a series of infants and preschoolers with acute diarrhea.200212216520
seasonal prevalence of intestinal parasites in the united states during 2000.one-third of 5,792 fecal specimens from 2,896 patients in 48 states and the district of columbia tested positive for intestinal parasites during the year 2000. multiple infections with 2-4 parasitic species constituted 10% of 916 infected cases. blastocystis hominis infected 662 patients (23% or 72% of the 916 cases). its prevalence appears to be increasing in recent years. eighteen other species of intestinal parasites were identified. cryptosporidium parvum and entamoeba histolytica/e. dispar ...200212224595
a cross-sectional study of intestinal parasitic infections among schoolchildren in nan province, northern thailand.a cross-sectional study of the prevalence of intestinal parasitic infections at eight schools in bo klau district and four schools in chalerm prakiet district, nan province, in january and february, 2001. a total of 1,010 fecal samples were examined using the formalin-ether sedimentation technique. results revealed that the rate of helminthic infection was 60.0%, while protozoa accounted for 36.2% of infections; mixed infections were common, resulting in a total prevalence of both parasites of 6 ...200212236415
[distribution of entero-parasitic infections in the peruvian highland: study carried out in six rural communities of the department of puno, peru].a prevalence study was carried out in six rural communities in the peruvian highlands with the purpose of achieving a better understanding of the distribution of entero-parasites. the communities were located along the banks of lake titicaca, in the provinces of puno and el collao, department of puno, peru, at 3,800 m.a.s.l. to that effect, a total of 91 feces samples of adults and children from the following communities were analyzed: conchaca, puñutani, capalla, culta, maraesqueña, and jaillih ...200212525846
prevalence of intestinal parasitic pathogens in hiv-seropositive individuals in northern india.to assess the prevalence of intestinal parasitic infections in human immunodeficiency virus (hiv)-seropositive subjects, fecal samples were collected from 120 hiv-seropositive patients and were analyzed for various intestinal parasites. thirty-six patients (30%) were found to harbor an intestinal parasite. cryptosporidium parvum was the most common (10.8%), followed by giardia lamblia (8.3%). cyclospora cayetanensis and blastocystis hominis each were detected in 3.3% of the patients, while isosp ...200212195048
palmoplantar pruritus subsiding after blastocystis hominis eradication. 200212013204
evaluation of an immunochromatographic dip-strip test for the detection of cryptosporidium oocysts in stool specimens.the study presented here examined the efficacy of a commercially available qualitative immunochromatographic assay for detecting cryptosporidium oocysts in stool samples. a total of 75 samples were tested, including 50 positive for cryptosporidium spp. by acid-fast stain, 20 positive for other parasites ( blastocystis hominis, endolimax nana, entamoeba coli, giardia lamblia, ascaris lumbricoides, strongyloides stercoralis and trichuris trichiura), and five negative samples. the observed sensitiv ...200212226697
epidemiological survey of blastocystis hominis in huainan city, anhui province, china.to provide scientific evidence for prevention and controlling of blastocystosis, the infection of blastocystis homonis and to study its clinical significance in huainan city, anhui province, china.200212378644
[morphological and ultrastructural observation of blastocystis hominis].to observe the morphology and ultrastructure of blastocystis hominis.200112571946
chronic intermittent diarrhoea and fatigue. 200111676320
ribodemes of blastocystis hominis isolated in japan.to determine if genetic diversity of blastocystis hominis exists in japan, we monitored 64 b. hominis-infected people: 39 asymptomatic people whose infections were detected during routine medical check-ups (32 japanese and 7 non-japanese) and 25 patients with gastrointestinal symptoms who visited the outpatient clinics of st. luke's international hospital (19 japanese and 6 non-japanese). we detected 6 known and 2 new riboprint patterns in isolates from the infected people. there were no differe ...200111693890
blastocystis hominis: a new pathogen in day-care centres? 200111381629
cryptosporidiosis in kuwaiti children: seasonality and endemicity.to detect the incidence of cryptosporidial infection in children presenting with gastrointestinal symptoms at the local hospital in kuwait.200111422253
morphology, histochemistry and infectivity of blastocystis hominis cyst.different morphological forms of blastocystis hominis had been identified in human stool samples. these included both cystic and trophic stages. the latter was induced to encyst by keeping them in potassium dichromate solution for two weeks. suspected of being the infective stage, cysts were studied in more detail as regards their morphology using both light and electron microscopy. histochemistry and infectivity studies were also carried out. light microscopy revealed the cysts to be ovoid or r ...200111478461
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