Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| utility of (11)c-methionine and (11)c-donepezil for imaging of staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)in-labelled leukocytes, (99m) tc-dpd, and (18)f-fdg. | the aim of this study was to compare (11)c-methionine and (11)c-donepezil positron emission tomography (pet) with (111)in-labeled leukocyte and (99m) tc-dpd (tc-99m 3,3-diphosphono-1,2-propanedicarboxylic acid) single-photon emission computed tomography (spect), and (18)f-fluorodeoxyglucose ((18)f-fdg) pet to improve detection of osteomyelitis. the tracers' diagnostic utility where tested in a juvenile porcine hematogenously induced osteomyelitis model comparable to osteomyelitis in children. fi ... | 2016 | 28078182 |
| biodistribution of the radionuclides (18)f-fdg, (11)c-methionine, (11)c-pk11195, and (68)ga-citrate in domestic juvenile female pigs and morphological and molecular imaging of the tracers in hematogenously disseminated staphylococcus aureus lesions. | approximately 5-7% of acute-care patients suffer from bacteremia. bacteremia may give rise to bacterial spread to different tissues. conventional imaging procedures as x-ray, computed tomography (ct), magnetic resonance imaging (mri), and ultrasound are often first-line imaging methods for identification and localization of infection. these methods are, however, not always successful. early identification and localization of infection is critical for the appropriate and timely selection of thera ... | 2016 | 27069765 |
| evolutionary diversity of suid herpesvirus 1 based on ul44 partial sequences. | the aim of this study was to use partial ul44 sequences (glycoprotein c) of suid herpesvirus 1 to examine the evolution and dynamics of the virus in different periods and hosts. | 2016 | 27362774 |
| intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine. | intradermal (id) vaccination constitutes a promising approach to induce anti-infectious immunity. this route of immunization has mostly been studied with influenza split-virion vaccines. however, the efficacy of id vaccination for sub-unit vaccines in relation to underlying skin innate immunity remains to be explored for wider application in humans. relevant animal models that more closely mimic human skin immunity than the widely used mouse models are therefore necessary. here, we show in domes ... | 2016 | 26768129 |
| pseudorabies virus isolates from domestic pigs and wild boars show no apparent in vitro differences in replication kinetics and sensitivity to interferon-induced antiviral status. | pseudorabies virus is the causative agent of aujeszky's disease. domestic pigs and wild boars are its natural hosts, and strains circulating within both populations differ in their capacity to induce clinical disease. cell biological and molecular explanations for the observed differences in virulence are, however, lacking. different virulence determinants that can be assessed in vitro were determined for five domestic swine strains, four wild boar strains and the nia3 reference strain. replicat ... | 2016 | 26590089 |
| age- and strain-dependent differences in the outcome of experimental infections of domestic pigs with wild boar pseudorabies virus isolates. | although pseudorabies virus (prv) has been eradicated in domestic swine in many countries, its presence in wild boars remains a threat for a reintroduction into the currently unprotected swine population. to assess the possible impact of such a reintroduction in a naive herd, an in vivo infection study using two genetically characterized wild boar prv isolates (bel24043 and bel20075) representative for wild boar strains circulating in south-western and central europe and the virulent nia3 refere ... | 2016 | 26589961 |
| porcine epidemic diarrhea virus and discovery of a recombinant swine enteric coronavirus, italy. | porcine epidemic diarrhea virus (pedv) has been detected sporadically in italy since the 1990s. we report the phylogenetic relationship of swine enteric coronaviruses collected in italy during 2007-2014 and identify a drastic shift in pedv strain variability and a new swine enteric coronavirus generated by recombination of transmissible gastroenteritis virus and pedv. | 2016 | 26689738 |
| a recombinant rabies virus (erags) for use in a bait vaccine for swine. | rabies viruses (rabv) circulating worldwide in various carnivores occasionally cause fatal encephalitis in swine. in this study, the safety and immunogenicity of a recombinant rabies virus, the erags strain constructed with a reverse genetics system, was evaluated in domestic pigs. | 2016 | 27489807 |
| heterogeneous antigenic properties of the porcine reproductive and respiratory syndrome virus nucleocapsid. | porcine reproductive and respiratory syndrome virus (prrsv) is an arterivirus responsible for a widespread contagious disease of domestic pigs with high economic impact. switzerland is one of the rare prrsv-free countries in europe, although sporadic outbreaks have occurred in the past. the prrsv isolate ivi-1173 from the short outbreak in switzerland in 2012 was entirely sequenced, and a functional full-length cdna clone was constructed. genetic and antigenic characterization of ivi-1173 reveal ... | 2016 | 27871316 |
| mortality due to porcine reproductive and respiratory syndrome virus in immunocompromised göttingen minipigs (sus scrofa domestica). | porcine reproductive and respiratory syndrome virus (prrsv) infection was diagnosed in 6 göttingen minipigs (sus scrofa domestica) with severe interstitial pneumonia. the virus was defined as a north american (na) subtype virus, which is common in the commercial pig population and might be derived from a widely used attenuated live-virus vaccine in europe. the orf5 sequence of the isolated prrsv was 98% identical to the vaccine virus. the affected pigs were part of a lung transplantation model a ... | 2016 | 27780006 |
| constant hepatitis e virus (hev) circulation in wild boar and red deer in spain: an increasing concern source of hev zoonotic transmission. | hepatitis e is a viral zoonosis that affects multiple hosts. the complete dynamics of infection in wildlife are still unknown, but the previous fact facilitates the maintenance and circulation of the virus, posing a risk to human health in the case of meat consumption from susceptible animals. in spain, it has been shown how domestic pigs, cattle and wildlife (i.e. wild boar and red deer) clearly interact in hunting farms, generating a complex epidemiological situation in terms of interspecies p ... | 2016 | 25571944 |
| prevalence of antibody to and dna of lawsonia intracellularis in samples from wild animals in korea. | we evaluated the prevalence of lawsonia intracellularis infection in three wild animal species in korea; the korean water deer ( hydropotes inermis ), siberian roe deer ( capreolus pygargus ), and raccoon dogs ( nyctereutes procyonoides ). we collected 136 sera and 109 fecal samples from individuals in 10 wildlife rescue and conservation centers. serum samples were tested for anti- l. intracellularis antibodies using a blocking enzyme-linked immunosorbent assay (belisa), and fecal samples were s ... | 2016 | 27487235 |
| hepatitis e: an emerging global disease - from discovery towards control and cure. | hepatitis e is a systemic disease affecting the liver predominantly and caused by infection with the hepatitis e virus (hev). hev has marked genetic heterogeneity and is known to infect several animal species including pigs, boar, deer, mongoose, rabbit, camel, chicken, rats, ferret, bats and cutthroat trout. hev is the sole member of the family hepeviridae and has been divided into 2 genera: orthohepevirus (mammalian and avian hev) and piscihepevirus (trout hev). human hevs included within the ... | 2016 | 26344932 |
| experimental evidence of hepatitis a virus infection in pigs. | hepatitis a virus (hav) is the leading cause of acute viral hepatitis worldwide, with hav infection being restricted to humans and nonhuman primates. in this study, hav infection status was serologically determined in domestic pigs and experimental infections of hav were attempted to verify hav infectivity in pigs. antibodies specific to hav or hav-like agents were detected in 3.5% of serum samples collected from pigs in swine farms. when the pigs were infected intravenously with 2 × 10(5) 50% t ... | 2016 | 26381440 |
| [detection of salmonella choleraesuis var. kunzendorf in a fattening pig with septicaemic salmonellosis. a case report]. | the present case reports the detection of salmonella (s.) choleraesuis var. kunzendorf and porcine circovirus type 2 in an organic fattening pig suffering from septicaemic salmonellosis and porcine dermatitis and nephropathy syndrome. six weeks after pigs had been housed in an organic fattening farm, an increase in mortality, diarrhea and coughing was observed. in recent years, s. choleraesuis var. kunzendorf has been frequently detected in wild boars in germany, whereas the same serovar did not ... | 2016 | 27808343 |
| latent porcine circovirus type 2-infected domestic pigs: a potential infection model for the effective development of vaccines against latent or chronic virus induced diseases. | until recently, knowledge of the pathogenicity of circoviridae and anelloviridae family members was limited. our previous discoveries provided clues toward resolving this issue based on studies of the latent nature of porcine circovirus type 2 (pcv2) genotype group members. we developed a conventional pig infection model that indicated that weaners already harbored latent pcv2 infection in the thymus, which enabled the viruses to specifically modulate the maturation of t-helper cells. this findi ... | 2016 | 26795369 |
| lung parasites of the genus metastrongylus molin, 1861 (nematoda: metastrongilidae) in wild boar (sus scrofa l., 1758) in central-italy: an eco-epidemiological study. | the respiratory tracts of 57 wild boars (sus scrofa l. 1758) hunted in central italy during the 2011/2012 hunting season were examined to detect the presence of lung worms. fifty-five out of 57 animals (96,5%) were positive. five species of metastrongylus were detected and their prevalence was as follows: metastrongylus asymmetricus noda, 1973 (91.2%), metastrongylus confusus jansen, 1964 and metastrongylus salmi gedoelst, 1923 (87.7%), metastrongylus apri gmelin, 1790 (80.7%), metastrongylus pu ... | 2016 | 26827860 |
| feral swine virome is dominated by single-stranded dna viruses and contains a novel orthopneumovirus which circulates both in feral and domestic swine. | feral swine are known reservoirs for various pathogens that can adversely affect domestic animals. to assess the viral ecology of feral swine in the usa, metagenomic sequencing was performed on 100 pooled nasal swabs. the virome was dominated by small, ssdna viruses belonging to the families circoviridae, anelloviridae and parvovirinae. only four rna viruses were identified: porcine kobuvirus, porcine sapelovirus, atypical porcine pestivirus and a novel orthopneumovirus, provisionally named swin ... | 2016 | 27417702 |
| a comprehensive microbiological safety approach for agarose encapsulated porcine islets intended for clinical trials. | the use of porcine islets to replace insulin-producing islet β-cells, destroyed during the diabetogenic disease process, presents distinct challenges if this option is to become a therapeutic reality for the treatment of type 1 diabetes. these challenges include a thorough evaluation of the microbiological safety of the islets. in this study, we describe a robust porcine islet-screening program that provides a high level of confidence in the microbiological safety of porcine islets suitable for ... | 2016 | 27862363 |
| molecular detection of porcine astrovirus in sichuan province, china. | porcine astrovirus (poastv) is widely distributed worldwide, and is highly prevalent among piglets with or without diarrhea, existing as at least five distinct lineages (poastv1-poastv5) within the genus mamastrovirus. however, our knowledge of the diversity and epidemiology of poastv in china is limited. | 2016 | 26739067 |
| molecular survey of blastocystis sp. from humans and associated animals in an indonesian community with poor hygiene. | blastocystis sp. is a common parasite found in human and animal fecal samples. currently, human blastocystis isolates are classified into nine subtypes (sts) based on the phylogeny of their small subunit ribosomal rna genes (ssu rdnas). since eight of the nine sts, except for st9, have been reported in both humans and animals, these parasites are considered to be potentially zoonotic sts. to evaluate whether zoonotic transmissions play a main role in the lifecycle of blastocystis, sts derived fr ... | 2016 | 27080248 |
| improved experimental model to evaluate submucosal injection solutions for endoscopic submucosal dissection. | endoscopic submucosal dissection carries an increased risk of bleeding and perforation. the creation of a long lasting submucosal cushion is essential for the safe and complete removal of the lesion. there is not a suitable experimental model for evaluation of the durability of the cushioning effect of different solutions. | 2016 | 26734797 |
| multispecies purification of testicular germ cells. | advanced methods of cellular purification are required to apply genome technology to the study of spermatogenesis. one approach, based on flow cytometry of murine testicular cells stained with hoechst-33342 (ho-facs), has been extensively optimized and currently allows the isolation of 9 germ cell types. this staining technique is straightforward to implement, highly effective at purifying specific germ cell types and yields sufficient cell numbers for high throughput studies. ho-facs is a techn ... | 2016 | 27557646 |
| dynamics of necrophagous insect and tissue bacteria for postmortem interval estimation during the warm season in romania. | the estimation of postmortem interval (pmi) is affected by several factors including the cause of death, the place where the body lay after death, and the weather conditions during decomposition. given the climatic differences among biogeographic locations, the understanding of necrophagous insect species biology and ecology is required when estimating pmi. the current experimental model was developed in romania during the warm season in an outdoor location. the aim of the study was to identify ... | 2016 | 26487246 |
| sheep carrying pathogenic yersinia enterocolitica bioserotypes 2/o:9 and 5/o:3 in the feces at slaughter. | yersinia enterocolitica is a heterogeneous species including non-pathogenic strains belonging to biotype 1a and pathogenic strains belonging to biotypes 1b and 2-5. pathogenic strains of biotypes 2-4 carrying the ail virulence gene have frequently been isolated from domestic pigs at slaughter. in sheep, mostly non-pathogenic biotype 1a strains have been reported. in our study, the prevalence of ail-positive y. enterocolitica was studied by pcr and culturing in 406 young sheep (<1year of age) and ... | 2016 | 27938687 |
| bee pollen as a promising agent in the burn wounds treatment. | the aim of the present study was to visualize the benefits and advantages derived from preparations based on extracts of bee pollen as compared to pharmaceuticals commonly used in the treatment of burns. the bee pollen ointment was applied for the first time in topical burn treatment. experimental burn wounds were inflicted on two white, domestic pigs. clinical, histopathological, and microbiological assessment of specimens from burn wounds, inflicted on polish domestic pigs, treated with silver ... | 2016 | 27293466 |
| erythrocytes from ggta1/cmah knockout pigs: implications for xenotransfusion and testing in non-human primates. | pig erythrocytes are potentially useful to solve the worldwide shortage of human blood for transfusion. domestic pig erythrocytes, however, express antigens that are bound by human preformed antibodies. advances in genetic engineering have made it possible to rapidly knock out the genes of multiple xenoantigens, namely galactose α1,3 galactose (agal) and n-glycolylneuraminic acid (neu5gc). we have recently targeted the ggta1 and cmah genes with zinc finger endonucleases resulting in double knock ... | 2016 | 24986655 |
| cross-sectional study of anti-trichinella antibody prevalence in domestic pigs and hunted wild boars in estonia. | trichinella spp. are relevant zoonotic pathogens in estonia. the aim of this nationwide cross-sectional study was to estimate the seroprevalence of trichinella spp. in domestic pigs (sus scrofa domestica) and hunted wild boars (sus scrofa). serum samples from 374 pigs, originating from 14 farms, and meat juice samples from 470 wild boars were tested for immunoglobulin g antibodies against trichinella excretory/secretory antigens using a commercial enzyme-linked immunosorbent assay (elisa). antib ... | 2016 | 27315523 |
| genetics of adiposity in large animal models for human obesity-studies on pigs and dogs. | the role of domestic mammals in the development of human biomedical sciences has been widely documented. among these model species the pig and dog are of special importance. both are useful for studies on the etiology of human obesity. genome sequences of both species are known and advanced genetic tools [eg, microarray snp for genome wide association studies (gwas), next generation sequencing (ngs), etc.] are commonly used in such studies. in the domestic pig the accumulation of adipose tissue ... | 2016 | 27288831 |
| historical incidence of spontaneous lesions in kidneys from naïve swine utilized in interventional renal denervation studies. | the use of preclinical animal models is integral to the safety assessment, pathogenesis research, and testing of diagnostic technologies and therapeutic interventions. with inherent similarity to human anatomy and physiology, various porcine models have been the preferred preclinical model in some research areas such as medical devices, wound healing, and skin therapies. the porcine model has been the cornerstone for interventional cardiology for the evaluation and development of this catheter-b ... | 2016 | 27188879 |
| conserved differences in protein sequence determine the human pathogenicity of ebolaviruses. | reston viruses are the only ebolaviruses that are not pathogenic in humans. we analyzed 196 ebolavirus genomes and identified specificity determining positions (sdps) in all nine ebolavirus proteins that distinguish reston viruses from the four human pathogenic ebolaviruses. a subset of these sdps will explain the differences in human pathogenicity between reston and the other four ebolavirus species. structural analysis was performed to identify those sdps that are likely to have a functional e ... | 2016 | 27009368 |
| preparation and characterization of an antibody antagonist that targets the porcine growth hormone receptor. | a series of antagonists specifically targeting growth hormone receptors (ghr) in different species, such as humans, rats, bovines, and mice, have been designed; however, there are currently no antagonists that target the porcine growth hormone (gh). therefore, in this study, we developed and characterized a porcine ghr (pghr) antibody antagonist (denoted by an98) via the hybridoma technique. the results from enzyme-linked immunosorbent assay, fluorescence activated cell sorter, indirect immunoin ... | 2016 | 26954133 |
| temporal stability of personality traits in group-housed gestating sows. | the movement of sows (sus scrofa domesticus) out of individual gestation stalls and into group housing can introduce new sources of stress due to the enhanced environmental and social complexity. some sows may have the behavioral capacity to adapt to these changes better than others. however, little is known about individual differences in behavioral responses, or personality traits, in gestating sows and how they impact the animal's ability to cope with group housing. the temporal consistency i ... | 2016 | 26915682 |
| the production of multi-transgenic pigs: update and perspectives for xenotransplantation. | the domestic pig shares many genetic, anatomical and physiological similarities to humans and is thus considered to be a suitable organ donor for xenotransplantation. however, prior to clinical application of porcine xenografts, three major hurdles have to be overcome: (1) various immunological rejection responses, (2) physiological incompatibilities between the porcine organ and the human recipient and (3) the risk of transmitting zoonotic pathogens from pig to humans. with the introduction of ... | 2016 | 26820415 |
| on-pump nonrobotic total endoscopic coronary artery bypass grafting: an animal study. | total endoscopic coronary artery bypass grafting (tecab) is currently performed exclusively with the help of a telemanipulator. after extensive practice in a biomechanical model, the operation was performed as a nonrobotic approach in an animal study. | 2016 | 26331233 |
| geometric features of pig airways using computed tomography. | accurate knowledge of the airway geometry is needed when constructing physical models of the airway tree and for numerical modeling of flow or sound propagation in the airways. human and animal experiments are conducted to validate these models. many studies documented the geometric details of the human airways. however, information about the geometry of pig airways is scarcer. earlier studies suggested that the morphology of animal airways can be significantly different from that of humans. the ... | 2016 | 27798351 |
| n-linked glycan profiling of ggta1/cmah knockout pigs identifies new potential carbohydrate xenoantigens. | the temporary or long-term xenotransplantation of pig organs into people would save thousands of lives each year if not for the robust human antibody response to pig carbohydrates. genetically engineered pigs deficient in galactose α1,3 galactose (gene modified: ggta1) and n-glycolylneuraminic acid (gene modified: cmah) have significantly improved cell survival when challenged by human antibody and complement in vitro. there remains, however, a significant portion of human antibody binding. | 2016 | 24033743 |
| genomewide association studies for hematological traits and t lymphocyte subpopulations in a duroc × erhualian f resource population. | it has been shown that hematological traits can act as important indicators of immune function in both humans and livestock. t lymphocytes are key components of the adaptive immune system, playing a critical role in immune response. to identify genomic regions affecting hematological traits and t lymphocyte subpopulations, we performed both a snp-based genomewide association study (gwas) and a haplotype analysis for 20 hematological traits and 8 t cell subpopulations at 3 different time points ( ... | 2016 | 28046140 |
| radiologic advantages of potential use of polymer plastic clips in neurosurgery. | plastic clips are made of diamagnetic material and may result in fewer computed tomography (ct) and magnetic resonance artifacts than titanium clips. considering that polymer plastic clips are increasingly being used in endoscopic surgery, our study examined the ct and magnetic resonance imaging (mri) characteristics of plastic clips after application in the neurocranium and compared them with titanium clips. | 2016 | 24067738 |
| population phylogenomic analysis and origin of mitochondrial dna in chinese domestic pig. | the genetic diversity of eight domestic pigs was analyzed using a hypervariable fragment in the mitochondrial (mt) dna control region; a portion of the hypervariable control region (515 bp) was sequenced from 153 samples. haplotype diversity and nucleotide diversity in yushan black pig populations were significantly higher than other populations (p < 0.01). a neighbor-joining tree was constructed from domestic pig mtdna and five wild pigs. the results indicate that there are only small differenc ... | 2016 | 24865908 |
| detecting mitochondrial signatures of selection in wild tibetan pigs and domesticated pigs. | selection in genomic regions is prevalent in mammals; however, the effects of selection on the mitogenome are not clearly understood. we determined the complete mitochondrial dna (mtdna) sequences from six wild tibetan pigs from the tibetan plateau and four domestic pig breeds from the lowland of neighboring southwest china. nucleotide diversity analysis using the sliding window method showed that the nucleotide diversity of wild tibetan pigs in most regions of the mitogenome was higher than tha ... | 2016 | 24810064 |
| quantitative assessment of atrial regional function using motion estimation computed tomography. | we validated a novel image-based motion estimation computed tomographic (ct) technique (ime) to quantify atrial regional function in swine in vivo. | 2016 | 24983435 |
| catheter based selective hypothermia reduces stroke volume during focal cerebral ischemia in swine. | total body hypothermia is an established neuroprotectant in global cerebral ischemia. the role of hypothermia in acute ischemic stroke remains uncertain. selective application of hypothermia to a region of focal ischemia may provide similar protection with more rapid cooling and elimination of systemic side effects. we studied the effect of selective endovascular cooling in a focal stroke model in adult domestic swine. | 2016 | 25676148 |
| autophagy portends the level of cardiac hypertrophy in experimental hypertensive swine model. | left ventricular (lv) hypertrophy (lvh) plays an important role in hypertensive heart disease, and may be accompanied by myocardial autophagy. however, the pattern of autophagy during evolution of lvh is unclear. we hypothesized that autophagy activation indicates advancing cardiac lvh with tissue remodeling. | 2016 | 25904651 |
| detection of african swine fever antibodies in experimental and field samples from the russian federation: implications for control. | african swine fever (asf) re-entered in europe in 2007 by georgia rapidly affecting neighbouring countries. since then, asf has caused severe problems to the russian federation (rf) and spread to northern and western regions, including ukraine (2012 and 2014) and belarus (2013). at the beginning of 2014, dead wild boars were found in lithuania and poland. several outbreaks have been later notified in the european union(eu), affecting domestic pigs and wild boar of latvia, lithuania and poland, a ... | 2016 | 25440300 |
| histochemical study of heparin-positive mast cells in the terminal part of porcine ductus choledochus and papilla duodeni major. | the study presented in detail the localization and density of mast cells (mcs) in the intramural part of the common bile duct (cbd) and in the major duodenal papilla (mdp) of domestic swine. mcs' density (number/mm(2) ) in different layers of both of the duct and papilla was evaluated after toluidine blue staining. their number was higher in the lamina propria mucosae than in the tunica muscularis of the studied structures. the localization of berberine-positive, (heparin containing) mcs and the ... | 2016 | 26394797 |
| influence of the cutting edge angle of a titanium instrument on chip formation in the machining of trabecular and cortical bone. | the placement of self-tapping implants is associated with microfractures and the formation of bone chips along the cutting flutes. this study was conducted to investigate the effect of different cutting edge angles on chip formation during the machining of trabecular and cortical bone using instruments with a rough titanium surface. | 2016 | 25032776 |
| carcass mass has little influence on the structure of gravesoil microbial communities. | little is known about how variables, such as carcass mass, affect the succession pattern of microbes in soils during decomposition. to investigate the effects of carcass mass on the soil microbial community, soils associated with swine (sus scrofa domesticus) carcasses of four different masses were sampled until the 15th day of decomposition during the month of june in a pasture near lincoln, nebraska. soils underneath swine of 1, 20, 40, and 50 kg masses were investigated in triplicate, as well ... | 2016 | 26024793 |
| a novel swine model of spontaneous hypertension with sympathetic hyperactivity responds well to renal denervation. | the large animal model of arterial hypertension is very valuable to test the antihypertensive drugs and devices. we characterized a novel swine model of spontaneous hypertension and investigated its response to renal denervation (rdn). | 2016 | 25958303 |
| a nuclear magnetic resonance spectroscopy as a method for evaluation of in vivo poly-l-lactide biodegradation kinetics from stent-polymer matrices: an experimental study utilizing porcine model of in-stent restenosis. | we aimed to comprehensively evaluate poly-lactide polymer degradation and sirolimus release kinetics from a drug-eluting stent matrix in the in vivo setting using a nuclear magnetic resonance (nmr) method. | 2016 | 25944843 |
| genetic variation of the porcine nr5a1 is associated with meat color. | because of the central role of steroidogenic factor 1 in the regulation of the development and function of steroidogenic tissues, including the adrenal gland, we chose the encoding gene nr5a1 as a candidate for stress response, meat quality and carcass composition in the domestic pig. to identify polymorphisms of the porcine nr5a1 we comparatively sequenced the coding, untranslated and regulatory regions in four commercial pig lines. single nucleotide polymorphisms could be found in the 3' utr a ... | 2016 | 26055431 |
| the fate of human platelets exposed to porcine renal endothelium: a single-pass model of platelet uptake in domestic and genetically modified porcine organs. | thrombocytopenia may represent a significant challenge to the clinical application of solid-organ xenotransplantation. when studied in a pig-to-primate model, consumptive coagulopathy has challenged renal xenografts. new strategies of genetic manipulation have altered porcine carbohydrate profiles to significantly reduce human antibody binding to pig cells. as this process continues to eliminate immunologic barriers to clinical xenotransplantation, the relationship between human platelets and pi ... | 2016 | 26375504 |
| focused post mortem dissection technique for harvest of rete mirabile in domestic swine (sus scrofa). | rete mirabile (rm) of the domestic pig is a popular animal model of arteriovenous malformations. the rm (latin for 'wonderful net)' comprises the arterioarterial portal connecting ascending pharyngeal arteries and the internal carotid arteries, which exists in the skull base of even-toed ungulates. although angiographic access of the rm is relatively easy, its post mortem procurement is complicated and its detailed technique has not been well described. | 2016 | 26359213 |
| submerged flapless technique vs. conventional flap approach for implant placement: experimental domestic pig study with 12-month follow-up. | the aim of our study was to compare osseointegration and peri-implant crestal bone resorption in submerged flapless and conventional flap surgery over a 12-month follow-up. | 2016 | 26147852 |
| effect of elevated reperfusion pressure on "no reflow" area and infarct size in a porcine model of ischemia-reperfusion. | the "no reflow" phenomenon (microvascular obstruction despite restoration of epicardial blood flow) develops postreperfusion in acute myocardial infarction and is associated with poor prognosis. we hypothesized that increased reperfusion pressure may attenuate the no reflow phenomenon, as it could provide adequate flow to overcome the high resistance of the microvasculature within the no reflow zone. thus, we investigated the effect of modestly elevated blood pressure during reperfusion on the e ... | 2016 | 26589289 |
| pervasive environmental contamination with human feces results in high prevalence of zoonotic sarcocystis infection in pigs in the punjab, india. | three species of sarcocystis-s. miescheriana, s. suihominis, and s. porcifelis-have been recorded from pigs ( sus scrofa ). among these 3 species, the zoonotic species s. suihominis is of paramount importance and an important food safety issue. previous studies indicate prevalence of porcine sarcocystis species in india, but molecular evidence, among other evidence, is required for proper species differentiation. myocardium from 250 stray and farm pigs destined for slaughter for human consumptio ... | 2016 | 26588086 |
| articular cartilage repair with recombinant human type ii collagen/polylactide scaffold in a preliminary porcine study. | the purpose of this study was to investigate the potential of a novel recombinant human type ii collagen/polylactide scaffold (rhco-pla) in the repair of full-thickness cartilage lesions with autologous chondrocyte implantation technique (aci). the forming repair tissue was compared to spontaneous healing (spontaneous) and repair with a commercial porcine type i/iii collagen membrane (pco). domestic pigs (4-month-old, n = 20) were randomized into three study groups and a circular full-thickness ... | 2016 | 26573959 |
| fractional radiofrequency combined with sonophoresis to facilitate skin penetration of 5-aminolevulinic acid. | ablative fractional technology has been used to improve transdermal drug delivery. however, there have been few previous in vivo investigations of the relative potency and methodology of fractional radiofrequency (rf) combined with sonophoresis. the purpose of this study was to investigate the effects of fractional rf combined with sonophoresis on 5-aminolevulinic acid (ala) penetration of the skin. three male domestic swine were used. the skin of the pigs was exposed to fractional rf and/or son ... | 2016 | 26563955 |
| intraluminal measurement of papillary duct urine ph, in vivo: a pilot study in the swine kidney. | we describe the in vivo use of an optic-chemo microsensor to measure intraluminal papillary duct urine ph in a large mammal. fiber-optic ph microsensors have a tip diameter of 140-µm that allows insertion into papillary bellini ducts to measure tubule urine proton concentration. anesthetized adult pigs underwent percutaneous nephrolithotomy to access the lower pole of the urinary collecting system. a flexible nephroscope was advanced towards an upper pole papilla with the fiber-optic microsensor ... | 2016 | 26526044 |
| detection of genetic diversity and selection at the coding region of the melanocortin receptor 1 (mc1r) gene in tibetan pigs and landrace pigs. | domestication and subsequent selective pressures have produced a large variety of pig coat colors in different regions and breeds. the melanocortin 1 receptor (mc1r) gene plays a crucial role in determining coat color of mammals. here, we investigated genetic diversity and selection at the coding region of the porcine melanocortin receptor 1 (mc1r) in tibetan pigs and landrace pigs. by contrast, genetic variability was much lower in landrace pigs than in tibetan pigs. meanwhile, haplotype analys ... | 2016 | 26431999 |
| perirenal fat promotes renal arterial endothelial dysfunction in obese swine through tumor necrosis factor-α. | perirenal fat is associated with poor blood pressure control and chronic kidney disease but the underlying mechanisms remain elusive. we tested the hypothesis that perirenal fat impairs renal arterial endothelial function in pigs with obesity-metabolic derangements. | 2016 | 26417644 |
| percutaneous renal access: surgical factors involved in the acute reduction of renal function. | studies in patients and experimental animals have shown that percutaneous nephrolithotomy (pcnl) can acutely impair glomerular filtration and renal perfusion, but the factors contributing to this decline in renal function are unknown. the present study assessed the contribution of needle puncture of the kidney vs dilation of the needle tract to the acute decline in renal hemodynamic and tubular transport function associated with pcnl surgery. | 2016 | 26415144 |
| a novel hemostatic patch that stops bleeding in cardiovascular and peripheral vascular procedures. | achieving hemostasis during surgery is a common challenge across surgical specialties and procedures, including cardiovascular and peripheral vascular procedures. although several hemostatic agents are available, they all become less effective as bleeding becomes more vigorous. veriset™ hemostatic patch is a topical hemostatic agent that has been approved for use on solid organs and in soft tissue. the primary aim of these studies was to assess the safety of veriset hemostatic patch during cardi ... | 2016 | 26597233 |
| distinction of non-ischemia inducing versus ischemia inducing coronary stenosis by fluorescent cardiac imaging. | intraoperative fluorescent cardiac imaging (fci) can quantitatively assess myocardial perfusion abnormalities produced by graded flow-limiting coronary stenosis (fls), but there are no data to distinguish fls from non flow-limiting stenosis (nfls) to determine their functional significance. the aim of the study was to evaluate whether non-ischemia inducing nfls can be quantified and differentiated from fls by fci technology. data are compared to gold standard fluorescent microspheres (fm). 11 do ... | 2016 | 26408104 |
| domestication and cereal feeding developed domestic pig-type intestinal microbiota in animals of suidae. | intestinal microbiota are characterized by host-specific microorganisms, which have been selected through host-microbe interactions under phylogenetic evolution and transition of feeding behavior by the host. although many studies have focused on disease-related intestinal microbiota, the origin and evolution of host-specific intestinal microbiota have not been well elucidated. pig is the ideal mammal model to reveal the origin and evolution of host-specific intestinal microbiota because their d ... | 2016 | 26315488 |
| porcine survival model to simulate acute upper gastrointestinal bleedings. | the existing animal models used for the simulation of acute gastrointestinal bleedings are usually non-survival models. we developed and evaluated a new porcine model (domestic pig, german landrace) in which the animal remains alive and survives the artificial bleeding without any cardiovascular impairment. this consists of a bleeding catheter which is implanted into the stomach, then subcutaneously tunnelled from the abdomen to the neck where it is exteriorized and fixed with sutures. using the ... | 2016 | 26306615 |
| a pharmacologic activator of endothelial kca channels increases systemic conductance and reduces arterial pressure in an anesthetized pig model. | ska-31, an activator of endothelial kca2.3 and kca3.1 channels, reduces systemic blood pressure in mice and dogs, however, its effects in larger mammals are not well known. we therefore examined the hemodynamic effects of ska-31, along with sodium nitroprusside (snp), in anesthetized, juvenile male domestic pigs. experimentally, continuous measurements of left ventricular (lv), aortic and inferior vena cava (ivc) pressures, along with flows in the ascending aorta, carotid artery, left anterior d ... | 2016 | 26239885 |
| comparison of complement activation-related pseudoallergy in miniature and domestic pigs: foundation of a validatable immune toxicity model. | complement activation-related pseudoallergy (carpa) is an acute adverse immune reaction caused by many nanomedicines. there is a regulatory need for a sensitive and standardizable in vivo predictive assay. while domestic pigs are a sensitive animal model, miniature pigs are favored in toxicological studies yet their utility as a carpa model has not yet been explored. herein, we used liposomal doxorubicin and amphotericin b (doxil/caelyx and ambisome), cremophor el and zymosan as carpa triggers t ... | 2016 | 26767512 |
| aortic hemostasis and resuscitation: preliminary experiments using selective aortic arch perfusion with oxygenated blood and intra-aortic calcium coadministration in a model of hemorrhage-induced traumatic cardiac arrest. | selective aortic arch perfusion (saap) uses a thoracic aortic balloon occlusion catheter for heart and brain perfusion in cardiac arrest to achieve return of spontaneous circulation (rosc). saap with oxygenated stored blood was studied in a model of hemorrhage-induced cardiac arrest. the study hypothesis was that intra-aortic calcium coadministration would be required to maintain normal aortic arch blood ionized calcium during saap and to achieve rosc. | 2016 | 26766760 |
| hypercholesterolemia impairs nonstenotic kidney outcomes after reversal of experimental renovascular hypertension. | revascularization of a stenotic renal artery improves kidney function only in select patients with renovascular hypertension (ht) secondary to atherosclerosis. however, the effects of reversal of renovascular ht (rrht) on the nonstenotic kidney are unclear. we hypothesized that concurrent hypercholesterolemia (hc) attenuates nonstenotic kidney recovery. | 2016 | 26739189 |
| the swine plasma metabolome chronicles "many days" biological timing and functions linked to growth. | the paradigm of chronobiology is based almost wholly upon the daily biological clock, or circadian rhythm, which has been the focus of intense molecular, cellular, pharmacological, and behavioral, research. however, the circadian rhythm does not explain biological timings related to fundamental aspects of life history such as rates of tissue/organ/body size development and control of the timing of life stages such as gestation length, age at maturity, and lifespan. this suggests that another bio ... | 2016 | 26735517 |
| detection and genetic characterization of a novel parvovirus distantly related to human bufavirus in domestic pigs. | in this study, a novel parvovirus (strain swine/zsana3/2013/hun, kt965075) was detected in domestic pigs and genetically characterized by viral metagenomics and pcr methods. the novel parvovirus was distantly related to the human bufaviruses and was detected in 19 (90.5 %) of the 21 and five (33.3 %) of the 15 faecal samples collected from animals with and without cases of posterior paraplegia of unknown etiology from five affected farms and one control farm in hungary, respectively. swine/zsana ... | 2016 | 26733298 |
| closed-loop glycaemic control using an implantable artificial pancreas in diabetic domestic pig (sus scrofa domesticus). | the performance of a completely implantable peritoneal artificial pancreas (ap) has been demonstrated in principle in a live diabetic domestic pig. the device consists of a smart glucose-sensitive gel that forms a gateway to an insulin reservoir and is designed to both sense glucose and deliver insulin in the peritoneal cavity. it can be refilled with insulin via subcutaneous ports and surgery was developed to insert the ap. diabetes was induced with streptozotocin (stz), the device filled with ... | 2016 | 26691655 |
| implantation of a tissue-engineered neo-bile duct in domestic pigs. | extrahepatic bile duct injuries remain severe complications during cholecystectomies and often require reconstruction by bilioenteric anastomosis (i.e., hepaticojejunostomy), which comes with further long-term complications (e.g., recurring ascending cholangitis, secondary biliary cirrhosis). in the case of inherent extrahepatic biliary atresia or during liver transplant, artificial or engineered bile ducts could allow novel surgical strategies without the need for hepaticojejunostomy. | 2016 | 26684913 |
| large-scale screening and characterization of enteroviruses and kobuviruses infecting pigs in vietnam. | a recent survey of pigs in dong thap province, vietnam identified a high frequency of enterovirus species g (ev-g) infection (144/198; 72.7%). amongst these was a plethora of ev-g types (ev-g1, ev-g6 and four new types ev-g8-ev-g11). to better characterize the genetic diversity of ev-g and investigate the possible existence of further circulating types, we performed a larger-scale study on 484 pig and 45 farm-bred boar faecal samples collected in 2012 and 2014, respectively. all samples from the ... | 2016 | 26653281 |
| assessing the accuracy of cone-beam computerized tomography in measuring thinning oral and buccal bone. | the aim of this study was to assess the accuracy and reliability of cone-beam computerized tomography (cbct) in measuring thinning bone surrounding dental implants. three implants were inserted into the mandible of a domestic pig at 6 different bone thicknesses on the vestibular and the lingual sides, and measurements were recorded using cbct. the results were obtained, analyzed, and compared with areas without implants. our results indicated that the bone thickness and the neighboring implants ... | 2016 | 26645480 |
| surgical implantation and functional assessment of an invasive telemetric system to measure autonomic responses in domestic pigs. | the first aim of this study was to establish a surgical procedure to implant a new telemetric device for the continuous recording of electrocardiogram (ecg) and blood pressure (bp) in freely moving pigs. a second aim was the functional assessment of cardiovascular parameters, including heart rate variability (hrv) and blood pressure variability (bpv), so that these data could be used as the basis for the objective evaluation of autonomic activity and balance in different behavioural contexts. el ... | 2016 | 26626089 |
| an implantable sensor device measuring suture tension dynamics: results of developmental and experimental work. | knowledge about suture tension dynamics after laparotomy closure is limited due to the lack of adequate measurement tools. as a consequence, a miniaturized implantable sensor and data logger were developed and applied experimentally in a porcine model to measure suture tension dynamics after laparotomy closure. | 2016 | 26621138 |
| early atherosclerosis aggravates renal microvascular loss and fibrosis in swine renal artery stenosis. | renal function in patients with atherosclerosis and renal artery stenosis (aras) deteriorates more frequently than in nonatherosclerotic ras. we hypothesized that aras aggravates stenotic-kidney micro vascular loss compared to ras. domestic pigs were randomized to normal, ras, and aras (ras fed a high-cholesterol diet) groups (n = 7 each). ten weeks later stenotic-kidney oxygenation, renal blood flow, and glomerular filtration rate (gfr) were evaluated in vivo, and micro vascular density by micr ... | 2016 | 26879682 |
| behavioural and physiological measures indicate subtle variations in the emotional valence of young pigs. | in the study of animal emotions, emotional valence has been found to be difficult to measure. many studies of farm animals' emotions have therefore focussed on the identification of indicators of strong, mainly negative, emotions. however, subtle variations in emotional valence, such as those caused by rather moderate differences in husbandry conditions, may also affect animals' mood and welfare when such variations occur consistently. in this study, we investigated whether repeated moderate ave ... | 2016 | 26850291 |
| argon and xenon ventilation during prolonged ex vivo lung perfusion. | evidence supports the use of ex vivo lung perfusion (evlp) as a platform for active reconditioning before transplantation to increase the potential donor pool and to reduce the incidence of primary graft dysfunction. a promising reconditioning strategy is the administration of inhaled noble gases based on their organoprotective effects. our aim was to validate a porcine warm ischemic lung injury model and investigate postconditioning with argon (ar) or xenon (xe) during prolonged evlp. | 2016 | 26850183 |
| novel roles for kv7 channels in shaping histamine-induced contractions and bradykinin-dependent relaxations in pig coronary arteries. | voltage-gated kv7 channels are inhibited by agonists of gq-protein-coupled receptors, such as histamine. recent works have provided evidence that inhibition of vascular kv7 channels may trigger vessel contractions. in this study, we investigated how kv7 activity modulates the histamine-induced contractions in "healthy" and metabolic syndrome (mets) pig right coronary arteries (cas). we performed isometric tension and immunohistochemical studies with domestic, lean ossabaw, and mets ossabaw pig c ... | 2016 | 26844882 |
| minipigs in translational immunosafety sciences: a perspective. | the porcine immune system has been studied especially with regard to infectious diseases of the domestic pig, highlighting the economic importance of the pig in agriculture. recently, in particular, minipigs have received attention as alternative species to dogs or nonhuman primates in drug safety evaluations. the increasing number of new drug targets investigated to modulate immunological pathways has triggered renewed interest to further explore the porcine immune system. comparative immunolog ... | 2016 | 26839327 |
| blood lactate concentrations in göttingen minipigs compared with domestic pigs. | measurement of the blood lactate concentration is a useful monitoring tool during anesthesia of animals and people. recently, blood lactate has been used to monitor anesthetized pigs, but very little is known about variations in blood lactate concentrations in this species. we therefore evaluate the effects of breed (domestic pigs compared with göttingen minipigs), body weight (domestic pigs of 40 kg compared with 70 kg), type of anesthesia (inhalation compared with infusion) and surgery (minor ... | 2016 | 26817974 |
| electrical impedance tomography (eit) for quantification of pulmonary edema in acute lung injury. | assessment of pulmonary edema is a key factor in monitoring and guidance of therapy in critically ill patients. to date, methods available at the bedside for estimating the physiologic correlate of pulmonary edema, extravascular lung water, often are unreliable or require invasive measurements. the aim of the present study was to develop a novel approach to reliably assess extravascular lung water by making use of the functional imaging capabilities of electrical impedance tomography. | 2016 | 26796635 |
| aortic root haemodynamics following david procedure: numerical analysis of 3-dimensional haemodynamics. | the aim was to determine 3-dimensional (3d) geometrical deformation of the aortic root (aor) following the david procedure in order to evaluate local haemodynamical conditions of individual aor elements. | 2016 | 26792930 |
| penile injuries in wild and domestic pigs. | in boars, sexually motivated mounting can not only cause problems such as lameness, but penile injuries are also reported. the relevance of penis biting in boars is discussed controversially, but reliable data is missing. in the present study, boars ( n = 435) and barrows ( n = 85) from experimental farms were therefore evaluated for scars, fresh wounds and severe injuries of the penis. similarly, 321 boars from 11 farms specializing in pork production with boars, and 15 sexually mature wild boa ... | 2016 | 27023619 |
| endovascular mr-guided renal embolization by using a magnetically assisted remote-controlled catheter system. | purpose to assess the feasibility of a magnetically assisted remote-controlled (marc) catheter system under magnetic resonance (mr) imaging guidance for performing a simple endovascular procedure (ie, renal artery embolization) in vivo and to compare with x-ray guidance to determine the value of mr imaging guidance and the specific areas where the marc system can be improved. materials and methods in concordance with the institutional animal care and use committee protocol, in vivo renal artery ... | 2016 | 27019290 |
| serological prevalence of enteropathogenic yersinia spp. in pigs and wild boars from different production systems in the moravian region, czech republic. | human yersiniosis caused by pathogenic yersinia spp. is one of the most common reported zoonoses in the european union and pigs are considered as the major reservoir of these bacteria. serological testing represents a suitable method to obtain information about the prevalence of enteropathogenic yersinia spp. in food animals. the prevalence of antibodies against enteropathogenic yersinia spp. was studied in 319 slaughtered pigs and 135 wild boars from different production systems in the moravian ... | 2016 | 26982547 |
| cellular cardiomyoplasty into infracted swine's hearts by retrograde infusion through the venous coronary sinus: an experimental study. | the aim was to create a model of myocardial infarction with a borderline myocardial impairment which would enable evaluation of the retrograde cellular cardiomyoplasty through the venous coronary sinus in a large animal model. | 2016 | 26953214 |
| gelatin-poly(lactic-co-glycolic acid) scaffolds with oriented pore channel architecture - from in vitro to in vivo testing. | a gelatin-poly(lactic-co-glycolic acid), plga, composite scaffold, featuring a highly oriented pore channel structure, was developed as a template for articular cartilage regeneration. as a design principle the composite scaffold was optimized to contain only medical grade educts and accordingly no chemical cross linking agents or other toxicological relevant substances or methods were used. scaffolds were synthesized using a freeze structuring method combined with an electrochemical process fol ... | 2016 | 26952462 |
| the effect of head up cardiopulmonary resuscitation on cerebral and systemic hemodynamics. | chest compressions during cardiopulmonary resuscitation (cpr) increase arterial and venous pressures, delivering simultaneous bidirectional high-pressure compression waves to the brain. we hypothesized that this may be detrimental and could be partially overcome by elevation of the head during cpr. | 2016 | 26905388 |
| intranasal oxytocin administration in relationship to social behaviour in domestic pigs. | intranasal administration of oxytocin has been shown to alter positive and negative social behaviour. positive social behaviour in pigs (sus scrofa) may be expressed through gentle social nosing, and greater insight in the specific expression hereof might contribute to the current search for positive indicators of animal welfare. we investigated whether oxytocin alters social nosing and whether this is specific to nose-body or nose-nose contact. sixty-four focal female pigs of 13weeks of age (ou ... | 2016 | 27143253 |
| complete antegrade transapical deployment of a branched aortic arch endograft: a porcine feasibility study. | to describe and prove the concept of a technique for complete transapical deployment of a single-branch aortic arch endograft in a porcine model. | 2016 | 27090167 |
| olfactory sensitivity in mammalian species. | olfaction enables most mammalian species to detect and discriminate vast numbers of chemical structures called odorants and pheromones. the perception of such chemical compounds is mediated via two major olfactory systems, the main olfactory system and the vomeronasal system, as well as minor systems, such as the septal organ and the grueneberg ganglion. distinct differences exist not only among species but also among individuals in terms of their olfactory sensitivity; however, little is known ... | 2016 | 27070753 |
| identification of a novel bufavirus in domestic pigs by a viral metagenomic approach. | bufavirus is a single-stranded dna virus belonging to the genus protoparvovirus. this study reports the identification and characterization of a porcine bufavirus by a metagenomic approach, and a limited epidemiology investigation of bufavirus in six swine farms. a comparative genome analysis showed a similarity of 93 % to a hungarian porcine bufavirus. bayesian and maximum-likelihood analyses of genome sequences showed a close relationship of porcine bufaviruses to human and monkey bufaviruses. ... | 2016 | 27058516 |
| coating of intravascular balloon with paclitaxel prevents constrictive remodeling of the dilated porcine femoral artery due to inhibition of intimal and media fibrosis. | here we investigated how a coating of intravascular balloon with paclitaxel (drug-coated balloon; dcb, freeway™) impacted porcine peripheral artery vascular function and remodeling. domestic swine (n = 54) underwent percutaneous overstretch balloon dilation of femoral and iliac arteries, controlled by angiography and optical coherence tomography (oct). paclitaxel tissue uptake was measured at 1 h and 1, 3, and 9 days post-dilation. at these time-points and at 32 ± 2 days, vascular function of th ... | 2016 | 27388164 |
| experimental taphonomy: post-mortem microstructural modifications in sus scrofa domesticus bone. | bone is a highly specialised form of hard and rigid connective tissue whose histological structure undergoes post-mortem modifications. in taphonomic research, histological examination of bone thin sections is used to investigate these post-mortem microstructural changes in skeletal tissues. in this study, diagenetic modifications in pig skeletal remains (sus scrofa domesticus) which were exposed to different taphonomic conditions as part of a long-term, real-time experiment were examined under ... | 2016 | 27368073 |
| feasibility of three-dimensional placement of human therapeutic stem cells using the intracerebral microinjection instrument. | the ability to safely place viable intracerebral grafts of human-derived therapeutic stem cells in three-dimensional (3d) space was assessed in a porcine model of human stereotactic surgery using the intracerebral microinjection instrument (imi) compared to a conventional straight cannula. | 2016 | 27593216 |
| urinary bladder matrix scaffolds strengthen esophageal hiatus repair. | laparoscopic repair of the hiatal hernia is associated with a recurrence rate between 12% and 42% depending on the defect size. although the impact of hiatal reinforcement on long-term recurrence remains controversial, the main limitation of this approach has been the risk of adverse events related with the use of synthetic materials in the vicinity of the esophagus. | 2016 | 27565070 |