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simian t-lymphotropic virus type 1 (stlv-1) infection in wild yellow baboons (papio hamadryas cynocephalus) from mikumi national park, tanzania.serum and peripheral blood leukocytes from wild yellow baboons (papio hamadryas cynocephalus) were tested for the presence of stlv-1-specific antibodies and proviral dna. fourteen of 30 sera tested positive by radioimmunoprecipitation assay (ripa) with htlv-1. among 36 dna samples tested by pcr 15 were positive by double nested pcr for a fragment of the stlv-1 env gene, the most sensitive assay among pcr tests employed. of 30 animals that were tested both serologically and by pcr in only 1 case ...19979123842
complete nucleotide sequence of the new simian t-lymphotropic virus, stlv-ph969 from a hamadryas baboon, and unusual features of its long terminal repeat.a third type of primate t-lymphotropic virus, ptlv-l, with stlv-ph969 as a prototype, has recently been isolated from an african baboon (papio hamadryas). classification of this virus has been based on partial sequence analysis of cdna from a virus-producing cell line, ph969. we obtained the complete nucleotide sequence of this virus with a proviral genome of 8,916 bp. all major genes, homologous in all human t-cell lymphotropic virus (htlv)-related viruses, and their corresponding mrnas, includ ...19979188619
[quantitative patterns in the clinical manifestations of radiation sickness in large laboratory animals exposed to radiation at superlethal doses. the quantitative patterns in the manifestations of radiation sickness in monkeys].radiation sickness manifestations have been studied in two species (macaca fascicularis and papio hamadryas) exposed to electron and gamma-neutron radiations in a wide supralethal doses range. dose-response relationships have been calculated for mortality and some clinical manifestations of the intestinal and cerebral forms of radiation sickness. higher radiosensitivity of baboons in comparison with macaca fascicularis and clinically more dangerous damage due to electrons as compared to gamma-ne ...19979244532
[follow-up study of the evolution of the quantitative eeg in intraventricular infusion of nerve growth factor in an aged baboon].the animal model of aged monkeys is a good homologue of alzheimer's disease in humans, in which it has been shown that there is disproportionate slowing of the eeg when compared with healthy subjects paired for age. this is found both on the conventional eeg and quantitatively. in the latter, relative energy has been the measurement most commonly used for diagnosis and follow-up with various treatments. the follow-up parameters evaluated in this study were: absolute and relative energy of the qu ...19979244612
[study of the effectiveness of recombinant alpha2-interferon and its inducers in primates with orthopoxvirus infection].the interferon-inducing and antiviral activities of high-molecular interferon inducers larifan and rifastin and of gene engineering human alpha 2-interferon were studied in monkeys infected with smallpox. these agents appreciably prolonged the life span of infected monkeys. for green monkeys the drug efficacies were as follows: larifan protected 50%, rifastin 86%, and realderon 50% (if used for treatment) and 80% (if used for urgent prophylaxis) of animals from death; for papio hamadryas the eff ...19979304302
[morpho-immunologic phenotypes of t-cell non-hodgkin's malignant lymphoma in baboons and their connection with with t-lymphotropic retrovirus stlv-1].8 cytological types were detected among 58 cases of t-nhl of papio hamadryas according to human updated kiel classification (1988). 5 of them were of low grade (lymphocytic, prolymphocytic, t-zone, angioimmunoblastic, small cell pleomorphic) and 3-of high grade (medium and large cell pleomorphic, immunoblastic, large cell anaplastic cd30/ki-1+). there are differences in a tumor structure and generalization in baboon and human lymphomas in spite of significant similarities. stlv-1 presence (antib ...19979139601
[preparation and characteristics of cultured strains of hepatitis a virus from humans and monkeys].cultural strains of hepatitis a virus (hav) have been isolated from spontaneously infected macaca mulatta (hav-mm), papio hamadryas (hav-ph), african green monkeys cercopithecus aethiops (hav-ca), and patients (hav-h). the strains replicate in continuous cells lines agmk, 4647, vero, and frhk-4. agmk and 4647 cells are the most permissive at 32 degrees c. virus propagation was not associated with the cytopathic effect and could be detected by enzyme immunoassay (eia), immune electron microscopy ...19979182400
androgen effects on bioactive and immunoreactive gonadotrophin levels during puberty in male baboons.the effect of androgens on changes in circulating lh and fsh during pubertal development was examined longitudinally in a 3 year study in male hamadryas baboons. baboon lh and fsh were measured by a species-specific radioimmunoassay and bioactive lh (b-lh) was measured by the mouse in vitro leydig cell bioassay. control baboons (n = 5) progressed normally through puberty. eight baboons were castrated prepubertally; of these four received testosterone implants at the chronological age (ca) of cli ...19979364367
growth and hormone characteristics of pubertal development in the hamadryas baboon.the semi-longitudinal collection of growth measurements in male and female hamadryas baboons has enabled documentation of the timing of puberty and the development of sexually dimorphic growth patterns in body weight, crown-rump length (crl), limb lengths, and muscle mass. in addition, another sexually dimorphic characteristic appears to be the presence of a pubertal growth spurt in body weight, and possibly crl, in male but not female baboons. serum testosterone levels rose during male developm ...19979379482
effects of ketamine sedation on glucose clearance, insulin secretion and counterregulatory hormone production in baboons (papio hamadryas).since the effects of ketamine sedation seem to differ between subspecies of baboons, we assessed the endocrine response to an intravenous glucose tolerance test (ivgtt) in 12 hamadryas baboons. the first phase insulin secretion, basal insulin, and glucose levels, as well as the glucose clearance, were significantly lower in sedated baboons as compared to fully awake animals. glucagon and cortisol were significantly higher, while growth hormone was lower during ketamine sedation. papio hamadryas ...19979438225
phylogenetic analysis of a simian t lymphotropic virus type i from a hamadryas baboon. 19979390755
ontogenetic changes in genetic regulation of fetal morphometrics in baboons (papio hamadryas subspp.).it is known that different genes are expressed during ontogeny; however, it is unclear how variation in that expression is associated with changes in growth patterns. the objective of this study is to assess how genetic variation in fetal morphology changes with ontogeny in baboons. longitudinal measures of the head and femur (60 to 180 days gestation) were available for 892 pregnancies. we used a genetic model that allowed both the genetic and environmental variances (sigma 2g and sigma 2e) to ...19979353978
genetics of adult body mass and maintenance of adult body mass in captive baboons (papio hamadryas subspecies).adult body mass and changes in mass during an individual's life are important indicators of general health and reproductive fitness. therefore, characterization of the factors that influence normal variation in body mass has important implications for colony management and husbandry. the main objective of this study was to quantify the genetic contribution to adult body mass and its maintenance in baboons. intra-individual mean and variance in body mass were calculated from multiple weight measu ...19979261509
the response of group-housed baboons to three enrichment toys.the behaviour of group-housed baboons was compared before and after the provision of durable cage toys. one adult male hamadryas baboon and 13 adult female olive baboons living in a large enclosure were observed after they were given seven nylon bones, seven kong toys and seven plaque attackers. observations were conducted four times per week on each subject over a 6-week period. abnormal, cage-directed, inactive and self-directed behaviours all significantly decreased after the provision of the ...19979121112
cyclical changes in the renin-angiotensin-aldosterone system during the menstrual cycle of the baboon (papio hamadryas).this study characterizes the renin-angiotensin-aldosterone system during the normal menstrual cycle in the baboon. ten animals received a daily dose of an ace inhibitor or placebo in a randomized blind cross-over design. data were obtained during the mid-follicular and early luteal phases of normal non-pregnant menstrual cycles. all examinations and blood collections were performed with ketamine sedation: 7-kg by im injection. blood pressure was recorded by sphygmomanometer. serum ace activity w ...19968906605
low blood protein heterozygosity in zoo-bred hamadryas baboons.an electrophoretic study of erythrocyte allozymes and serum proteins representing 32 genetic loci in 32 hamadryas baboons (papio h.hamadryas) from cologne and frankfurt zoos revealed biallelic polymorphism in phosphoglucomutase (pgm), mannose phosphate isomerase (mpi) and transferrin (tf). polymorphism amounted to p = 0.097 over 31 loci and observed heterozygosity to h(o) = 0.011, indicating significant reduction from the respective gene diversity parameters encountered in free-living hamadryas ...19968653592
changes of extracellular matrix in a baboon (papio hamadryas) model of insulin dependent diabetes: studies using electron microscopy and x-ray diffraction techniques.extracellular matrix plays an important role in many physiological functions and its abnormalities are thought to play a key role in the pathogenesis of diabetic complications. in this paper we used the techniques of electron microscopy, immunostaining and x-ray diffraction to document some of the early events in the changes of extracellular matrix in a model of insulin dependent diabetes in baboons. our results show that thickening of basement membrane and enlargement of mesangium are demonstra ...19969031807
a unique design for ease of access and movement of captive papio hamadryas.baboons are widely used in biomedical research but their size and behaviour present potential problems with accessibility. a unique system has been developed that ensures ease of access to all animals in a colony of papio hamadryas. two distinct caging complexes were linked by a network of overhead races, that are connected to a physical restraint area where individual animals could be separated, restrained and weighed without being handled. access to and separation of individual family groups w ...19968938619
reproductive and neonatal outcomes in captive bred baboons (papio hamadryas).baboons are widely used in biomedical research. although it is widely held that papio hamadryas breed well in captivity, each established colony has a different reproductive success often hypothesised to be due to husbandry practices. the national baboon colony in australia is a unique colony that houses papio hamadryas to mimic that structure seen in the wild. in this article; we have analysed their reproductive parameters and neonatal outcomes. the success of the colony husbandry practices was ...19968906608
androgens regulate circulating levels of insulin-like growth factor (igf)-i and igf binding protein-3 during puberty in male baboons.to examine the role of androgens in initiating the pubertal rise in circulating insulin-like factor-i (igf-i) levels, a longitudinal study of puberty in 13 male hamadryas baboons was conducted over 3 yr. the five control baboons commenced puberty (initial testicular enlargement) at a mean +/- se age of 4.2 +/- 0.4 yr. another eight baboons were castrated prepubertally; of those four received testosterone pellets (dose equivalent: 12.5-50 mg every 6 weeks) implanted se from the time of puberty. b ...19968550796
hamadryas baboons papio hamadryas as maintenance hosts of schistosoma mansoni in saudi arabia.free ranging hamadryas baboons (papio hamadryas) in four localities in the west and north of saudi arabia were examined for natural infection with schistosoma mansoni. faecal examination revealed infection with s. mansoni on four occasions within one year (at a prevalence rate of 2.5-4.0%) in only one locality, the al-baha area. the eggs were viable, as shown by miracidial hatching tests, and were recorded at a density of 140-280 eggs/g of faeces (7000-14,000 eggs/day). post-mortem examination o ...19968765452
the presence of a divergent t-lymphotropic virus in a wild-caught pygmy chimpanzee (pan paniscus) supports an african origin for the human t-lymphotropic/simian t-lymphotropic group of viruses.we isolated a divergent simian t-lymphotropic virus (stlv) (strain pp1664) from a wild-caught african bonobo (pygmy chimpanzee, pan paniscus). molecular and phylogenetic characterization of this virus show that it reliably separates from the two well-established primate t-lymphotropic virus types, htlv-i/stlv-i (ptlv-i) and ptlv-ii, and from a third type isolated from an african-born papio hamadryas and designated by us as ptlv-l. four of eight bonobos kept at the antwerp zoo, belgium, showed an ...19968609475
organization of the epaxial muscles in terrestrial and arboreal primates.the erector spinae muscles of the patas monkey hamadryas baboon, and spider monkey were dissected and the origins and insertions were described in detail. in the two terrestrial monkeys, a well-developed thoracolumbar segment of the muscle was observed. this trait seems to correlate to the sagittal movement of this region required in the pronograde quadrupedal locomotion. on the other hand, the erector spinae muscle of the spider monkey is quantitatively not so well developed and the arrangement ...19968953747
fetal ultrasonography: normal biometric ranges in the baboon (papio hamadryas).normal biometric ranges for fetal growth in a captive breeding baboon (papio hamadryas) colony are described. measurements include crownrump length, biparietal diameter, binocular distance, head circumference, abdominal circumference, femur length and amniotic fluid index. the pattern of fetal growth is compared with other baboon subspecies and man. the uses and limitations of such data for breeding colony management and optimum utilisation of experimentally derived data are discussed.19969029398
conservation of human chromosome 18 in baboons (papio hamadryas): a linkage map of eight human microsatellites.a panel of 25 microsatellite loci known to map to human chromosome 18 was screened for length polymorphisms in a multigenerational pedigree of baboons (papio hamadryas). eight of these loci were polymorphic in the baboon pedigrees, with observed heterozygosity values ranging from 0.16 to 0.88. all eight loci show strong evidence of linkage. the most likely map order among the microsatellite loci for papio is completely compatible with the locus order found in the human genome, and sex difference ...19969067425
phylogenetic characterization of simian t lymphotropic virus type i (stlv-i) from the ethiopian sacred baboon (papio hamadryas). 19968835205
molecular features of the tspy gene of gibbons and old world monkeys.restriction pattern, chromosome localization and the sequence of the testis-specific gene, tspy, were investigated in the white-handed gibbon, agile gibbon, siamang, hamadryas baboon and japanese monkey. southern blot analysis showed the tspy gene to be male specific in the primates used and disclosed variability of restriction pattern in gibbons. fluorescence in situ hybridization demonstrated that the probe pptspy2372, biotinylated using polymerase chain reaction, is located as a slight signal ...19968939361
the three human t-lymphotropic virus type i subtypes arose from three geographically distinct simian reservoirs.to investigate the origin of human t-lymphotropic virus i (htlv-i), strains of diverse geographical origin were analysed. we sequenced the ltr and env genes of htlv-i strains from brazil, central african republic, taiwan and zaire, and the simian t-lymphotropic virus type i (stlv-i) strain phsu1 from a baboon from the sukhumi primate centre. we performed phylogenetic analyses using neighbour-joining, parsimony and maximum likelihood methods. three separate htlv-i clusters were identified intersp ...19968627240
questions on the evolution of primate t-lymphotropic viruses raised by molecular and epidemiological studies of divergent strains.in human and simian t-lymphotropic viruses (htlv and stlv), collectively referred to as primate t-lymphotropic viruses (ptlv), four distinct clades can be distinguished: ptlv-i, ptlv-ii, and the newly discovered divergent stlvs isolated from hamadryas baboons and from bonobos (pygmy chimpanzees). the hamadryas stlv is clearly distinct from types i and ii, in terms both of sequence divergence and of genomic structure, and would qualify as a separate type, provisionally called ptlv-l. the bonobo s ...19968797730
comparative antigenicity of recombinant wild-type staphylokinase (sakstar) and a selected mutant (sakstar.m38) in a baboon thrombolysis model.staphylokinase, a bacterial plasminogen activator, is a potent, highly fibrin-specific but antigenic thrombolytic agent in humans. in an effort to attenuate the antigenicity of wild-type staphylokinase (sakstar variant), 2 of its 3 immunodominant epitopes were altered by substituting clusters of 2 or 3 charged amino acids with alanine, yielding the mutant sakstar.m38 (k35a, e38a, k74a, e75a, r77a), which was less antigenic in inbred new zealand white rabbits. in the present study, groups of 6 ba ...19968761847
the genomic structure of a new simian t-lymphotropic virus, stlv-ph969, differs from that of human t-lymphotropic virus types i and ii.a new simian t-lymphotropic virus, stlv-ph969, was recently isolated from a wild-born hamadryas baboon. previous analysis had revealed that it differs sufficiently from the other htlv/stlvs to be considered a new type, provisionally designated primate t-lymphotropic virus-l. here we analyse a 3850 bp cdna fragment spanning the 3' part of the stlv-ph969 genome. the fragment encodes three major proteins: env, tax and rex. sequence comparison and phylogenetic analysis indicate that in general stlv- ...19968627239
a comparison of tspy genes from y-chromosomal dna of the great apes and humans: sequence, evolution, and phylogeny.the genes for testis-specific protein y (tspy) were sequenced from chimpanzee (pan troglodytes), gorilla (gorilla gorilla), orangutan (pongo pygmaeus), and baboon (papio hamadryas). the sequences were compared with each other and with the published human sequence. substitutions were detected at 144 of the 755 nucleotide positions compared. in overviewing five sequences, one deletion in human, four successive nucleotide insertions in orangutan, and seven deletions/insertions in baboon sequence we ...19968798990
interspecies transmission of macaque simian t-cell leukemia/lymphoma virus type 1 in baboons resulted in an outbreak of malignant lymphoma.an outbreak of malignant lymphoma has been observed in one of the baboon (papio hamadryas) stocks of sukhumi primate center. more than 300 cases in this "high-lymphoma stock" have been registered since 1967. human t-cell lymphotropic virus type 1 (htlv-1)-related virus was implicated as the etiologic agent of sukhumi baboon lymphoma. the origin of this virus remained unclear. two possibilities were originally considered: the origin could be baboon simian t-cell leukemia/lymphoma virus type 1 (st ...19968627684
nucleotide polymorphism, effective population size, and dispersal distances in the yellow baboons (papio hamadryas cynocephalus) of mikumi national park, tanzania.we investigated genetic variability and population structure in a natural population of yellow baboons (papio hamadryas cynocephalus) from mikumi national park, tanzania. the amount of nuclear dna polymorphism in a random sample of animals from four social groups was estimated by using southern blotting to detect restriction fragment length polymorphisms in five nuclear autosomal loci. the average nucleotide heterozygosity was estimated to be 0.0033. this level of dna polymorphism is higher than ...199631918472
heritability of age at first birth in captive olive baboons.the evolution of life history traits is a topic of growing interest in primatology. traits associated with fertility, such as age at menarche and age at first birth, have great significance for natural selection, and knowing the genetic basis of such demographic traits may improve our understanding of population dynamics. knowledge of the heritability of reproductive traits may also have practical implications for the management of captive breeding colonies. a maximum likelihood method was used ...199531936949
[development and study of the properties of immunoglobulin against ebola fever].immunoglobulin to ebola fever has been for the first time prepared from hyperimmune equine blood sera by alcohol fractionation after cohn. preclinical study of the physicochemical and immunobiological properties of immunoglobulin showed that it protects up to 100% papio hamadryas infected intramuscularly at doses of 110 to 29 ld50 ebola virus. scheme for the use of ebola immunoglobulin has been experimentally validated.19958686265
zoonotic intestinal parasites of hamadryas baboons papio hamadryas in the western and northern regions of saudi arabia.six hundred and thirty-three faecal samples were randomly collected and examined for ova and cysts of intestinal parasites from five groups of hamadryas baboons of different population densities, with different human contact and in different ecological conditions (al-baha, turabah and al-taif in south-western and al-rihat and al-akhal in north-western saudi arabia). nine parasites were recorded (giardia lamblia, entamoeba histolytica, escherichia coli, balantidium coli, enterobius sp., trichuris ...19958544227
a non-human primate study (baboon; papio hamadryas) to determine if a long-acting progestogen, levonorgestrel butanoate, combined with a long-acting androgen, testosterone buciclate, can suppress spermatogenesis: i. dose-finding study.this study in adult male baboons was conducted to establish the dose of a long-acting progestogen, levonorgestrel butanoate, and that of a long-acting androgen, testosterone buciclate, which, when combined, would achieve optimal and prolonged suppression of spermatogenesis. two intramuscular injections of levonorgestrel butanoate at 3-month intervals and in the dose range 1-8 mg/kg reduced sperm production and plasma concentrations of testosterone, lh and fsh for periods of up to 6 months. the s ...19957665213
a non-human primate study (baboon; papio hamadryas) to determine if a long-acting progestogen, levonorgestrel butanoate, combined with a long-acting androgen, testosterone buciclate, can suppress spermatogenesis: ii. efficacy study.two combined injections of levonorgestrel butanoate (4 mg/kg) and testosterone buciclate (8 mg/kg) at 3-month intervals to adult male baboons initiated a decrease in sperm concentration from baseline values of 490x10(6)/ml to minimum values of 17x10(6/ml. this suppression was sustained until week 32, during which time between one and three azoospermic samples were collected from each of four out of five treated baboons in the period 10-24 weeks. circulating plasma levels of lh and testosterone d ...19957665214
influence of neuropeptides on the processes of higher nervous activity in primates. the action of cyclic analogs of enkephalins on the behavior of the lower monkeys.the effect of two cyclic enkephalin analogs (ivs-43 and ivs-46) on the group and individual behavior of rhesus macaques and hamadryas baboons has been studied. a pronounced influence of ivs-46 on competitive and operant goal-directed behavior of the monkeys was identified.19958614479
failure of experimental infection of baboons (papio hamadryas) with the eggs of asian taenia.the infectivity of the eggs of asian taenia sp. for humans is not known. using baboons (papio hamadryas) as a model to extrapolate the findings, three animals were exposed per os with 1000, 10,000 and 50,000 infective eggs of the asian taenia sp. the serological, biochemical, haematological and parasitological (based on necropsy) results show that baboons are refractory to the infection. it is concluded that the asian taeniid eggs may fail to develop in man.19958583131
linkage mapping in papio baboons: conservation of a syntenic group of six markers on human chromosome 1.we have established multipoint genetic linkage among six loci in baboons (papio hamadryas). published pcr primers designed to amplify five human microsatellite loci were used to amplify homologous loci in 229 pedigreed baboons. southern blotting was used to type two rflps in a functional gene (anti-thrombin iii) in a subset of those animals. all six loci are known to map to human chromosome 1q, a region of the genome predicted by karyotype studies to be conserved in baboons. pairwise recombinati ...19958530033
effects of age, sex, and heredity on measures of bone mass in baboons (papio hamadryas).bone mass and bone density were estimated in 219 pedigreed baboons (papio hamadryas) by radiographic morphometry of the left second metacarpal. compact bone width (total bone width--medullary canal diameter) and bone ratio (compact bone width/total bone width) decreased with increasing age squared in both sexes. the heritability of medullary canal diameter was 0.64 +/- 0.11, of compact bone width was 0.40 +/- 0.15 and of bone ratio was 0.67 +/- 0.13. the results indicate baboons are a useful mod ...19958750499
[standardization of conditions for the evaluation of effectiveness of antibacterial drugs in pneumonic plague in sacred baboons].the signs of pneumonic plague in sacred baboons infected by aerosol are: fever, hurried breathing, depression and constantly increasing bacteremia. some infected animals isolate the plague microbes while coughing and thus could be a source of the infection. by the clinical and pathomorphological signs, pneumonic plague in sacred baboons is similar to that in humans which makes it possible to use the animals in the development of schemes for special prophylaxis and treatment of the disease. in ef ...19958593090
a baboon (papio hamadryas) model of insulin-dependent diabetes.over a period of four years, streptozocin has been used to induce diabetes in 10 baboons, all of whom are insulin dependent. we describe our experience with their husbandry, induction of diabetes, insulin therapy, metabolic control and growth rate. streptozocin dosage of 60 mg/kg readily induces hyperglycemia with minimal hepatic or renal toxicity. using a once daily injection of mixed short and intermediate acting insulins at a dosage of 2-4 u/kg, it is possible to maintain a degree of metaboli ...19957563008
establishment and characteristics of an interleukin-2-dependent stlv-1-producing lymphoid cell line, sph-7(t), obtained from papio hamadryas with malignant lymphoma. 19947842161
fetotoxicity of angiotensin-converting enzyme inhibition in primate pregnancy: a prospective, placebo-controlled study in baboons (papio hamadryas).serious concerns have been raised about angiotensin-converting enzyme inhibition in pregnancy. the central question remains: does toxicity of angiotensin-converting enzyme inhibition pertain to pregnant humans?19948092208
haemodynamic actions of a nitric oxide (edrf) synthesis inhibitor in conscious baboons (papio hamadryas).1. the haemodynamic effects of intravenous nitric oxide inhibitor, n-nitro-l-arginine (nola), were examined in four conscious non-restrained baboons (papio hamadryas). mean arterial pressure, (map), systemic vascular resistance (svr) and cardiac output (co) were measured at timed intervals up to 24 h after a bolus injection of nola. 2. n-nitro-l-arginine increased blood pressure in a dose-dependent manner up to 9.5 mg/kg. increases in blood pressure were accompanied by increases in svr and decre ...19947529664
biochemical heterozygosity and morphologic variation in a colony of papio hamadryas hamadryas baboons.this analysis examines the association between genetic heterozygosity and individual morphologic variation in a captive population of papio hamadryas hamadryas consisting of 403 juveniles and adults. the population structure of the colony was artificially generated and maintained and is thus rigorously defined. subpopulations delimited by age, sex, and degree of inbreeding are also explored. heterozygosity, as enumerated from six simple mendelian biochemical loci, is compared with the residual m ...199428564474
effect of protein diets on the renal function of baboons (papio hamadryas) with remnant kidneys: a 5-year follow-up.to assess the progression of renal disease and the effects of protein intake in a species phylogenically close to humans, 14 adolescent baboons (papio hamadryas) were subjected to infarction of one third of the left kidney and, 2 months later, to right nephrectomy. they were then randomized to a synthetic protein diet containing either 8% or 25% casein. hemodynamic and metabolic measurements were obtained in awake animals every 4 months. modest proteinuria developed immediately after left kidney ...19948311075
[the cytogenetic characteristics of b-lymphoid lines from papio hamadryas baboons and macaca arctoides brown macaques].a cytogenetic analysis of 9 permanent suspension cell lines was performed. cell lines were obtained from papio hamadryas and macaca arctoides cells by different methods. five cell lines originated from non-malignant parental cells. lines spg-5 and spg-6 were analysed at early stages of cultivation, with normal karyotypes being identified. in cell lines pl-11, mal-1 and mal-4 pathological clones with structural and numerical chromosomal rearrangements appeared only following a long-term cultivati ...19947900211
baboon and cotton-top tamarin b2m cdna sequences and the evolution of primate beta 2-microglobulin.nonhuman primates represent phylogenetic intermediates for studying the divergence of human and murine beta 2ms. we report the nucleotide sequences of b2m cdna clones from a baboon cell line, 26cb-1 (papio hamadryas; primates: cercopithecoidea), and a cotton-top tamarin cell line, 1605l (saguinus oedipus; primates: ceboidea). the baboon and tamarin b2m sequences indicate a very slow rate of b2m evolution in primates relative to that in murid rodents. phenotypic evolution of beta 2m has also been ...19948026986
infection of a yellow baboon with simian immunodeficiency virus from african green monkeys: evidence for cross-species transmission in the wild.many african primates are known to be naturally infected with simian immunodeficiency viruses (sivs), but only a fraction of these viruses has been molecularly characterized. one primate species for which only serological evidence of siv infection has been reported is the yellow baboon (papio hamadryas cynocephalus). two wild-living baboons with strong sivagm seroreactivity were previously identified in a tanzanian national park where baboons and african green monkeys shared the same habitat (t. ...19947966642
[the evaluation in hamadryas baboons of the possibility for the specific prevention of ebola fever].the protective role of virus-neutralizing antibody was demonstrated in ebola virus-infected animals (papio hamadryas) used in experiments on the development of passive humoral immunity by using specific immunoglobulin. two immunizations of the monkeys with purified concentrated ebola virus antigen with complete freund adjuvant was shown to confirm intensive immunity to subsequent challenge protecting 80% of the immunized animals. it is concluded that effective preparations may be developed for s ...19948017061
brainstem auditory evoked response development in preterm and term baboons (papio hamadryas).brainstem auditory evoked responses were recorded longitudinally from 11 neonatal baboons (papio hamadryas), 6 of which were preterm. recordings were made in unsedated animals from day 161 to day 362 after conception (term = 182 days). the pattern of development of both waveform morphology and of wave latency was consistent with that seen in the human neonate, with a rapid maturation of the response during the perinatal period, and then a slower development to adult values. brainstem conduction ...19947842507
observations on the ascending aortic fold in two chacma baboons (papio hamadryas ursinus).baboons (papio) were used to study the ascending aortic fold. the fold was present in two out of 20 hearts examined. its position and shape was comparable to that observed in human subjects. the principal difference was the absence of paraganglia. the functional role of the baboon fold is unclear.19947897641
a primate t-lymphotropic virus, ptlv-l, different from human t-lymphotropic viruses types i and ii, in a wild-caught baboon (papio hamadryas).searching for clues to the evolution of the primate t-lymphotropic viruses (ptlvs), which include the human and the simian t-lymphotropic viruses (htlv and stlv), we have identified another ptlv, which differs sufficiently from the known ptlv-i and ptlv-ii types to be designated here ptlv-l. the virus was isolated from a wild-born baboon (papio hamadryas) from eritrea. in a cdna library a 1802-bp-long fragment was identified that extends from the env region, including the complete transmembrane ...19947908445
recombinant growth hormone and insulin-like growth factor i do not alter gonadotrophin stimulation of the baboon testis in vivo.in vitro studies indicate a physiological role for insulin-like growth factor i (igf-i) in paracrine regulation of testicular function and recent clinical studies suggest a potential role for growth hormone (gh) and/or igf-i in the treatment of hypogonadotrophic states in males. this study aimed to examine the effects of pretreatment with recombinant human gh (rhgh) or rhigf-i on the response to gonadotrophins of the non-human primate testis in vivo. using a balanced latin square design with rep ...19947921230
[the immunological efficacy of francisella tularensis outer membranes for hamadryas baboons].the protective properties of the preparation of f. tularensis outer membranes (om), obtained from f. tularensis vaccine strain 15, were studied in experiments on hamadryas baboons challenged subcutaneously with f. tularensis virulent strain schu (nonarctic subspecies). the subcutaneous immunization with the om preparation prevented the development of clinically pronounced infection in more than 70% of the monkeys challenged with f. tularensis strain schu in a dose of 787 live microbial cells 30 ...19947941871
temporal organisation of action in baboons: comparisons with the temporal segmentation in chimpanzee and human behaviour.the durations of sequences of functionally related movements, or action units, were analysed in the baboons papio hamadryas and papio anubis. action units are completed within a narrow time span or temporal segment as found previously in pongids and humans. although the temporal segmentation is generally similar in the three species, baboons show several differences from both chimpanzees and humans. firstly, their temporal segments are shorter and less variable and the different sorts of action ...19947953607
[spontaneous coronavirus infection in monkeys].a high level of the spread of coronavirus (cv) infection among hamadryas baboons and macaques of different species (about 50%), both resident in the animal house and imported, has been established. the tropism of cv to the gastrointestinal and respiratory tracts has been demonstrated. the course of spontaneous cv infection is accompanied by enterocolitis and/or pneumonia with periodic exacerbations, or takes the inapparent form. cases of virus persistence have also been noted. infected macaques ...19947856336
helminthic infestation complicated by intussusception in baboons (papio hamadryas).two juvenile baboons presented with diarrhoea, which did not resolve completely following antibiotic therapy. ileal intussusception was identified at autopsy in both cases. trichuris was the only gastrointestinal pathogen for which evidence could be found. this helminth is well-recognized as a cause of intussusception in human infants, but the complication has not been reported previously in non-human primates. it is likely to be fatal if undiagnosed.19947967467
[a trial to produce an inactivated lassa fever vaccine].a single and double immunization of papio hamadryas monkeys with an inactivated concentrated purified adsorbed preparation of lassa virus in doses of 0.5-1.0 and 1.1-2.1 mg by protein, respectively, protected the animals against the development of the disease and occurrence of viremia after an intramuscular challenge with 0.4 pfu dose. upon aerogenic infection of monkeys protection of 50% of the animals was achieved by a single immunization with the inactivated preparation in a dose 1.1 mg by pr ...19938303891
[a new variant of the simian t-lymphotropic retrovirus type i (stlv-if) in the sukhumi colony of hamadryas baboons].polymerase chain reaction (pcr) was developed for the detection of simian t-lymphotropic virus type 1 (stlv-1) infection of p. hamadryas and direct sequencing using oligo-nucleotide primer pairs specific for the tax and env regions of the related human t-lymphotropic virus type 1 (htlv-1). excellent specificity was shown in the detection of stlv-1 provirus in infected baboons by pcr using htlv-1-derived primers. the nucleotide sequences of env 467bp and tax 159bp of the proviral genome (env posi ...19937905690
a primate model of monotypism in atherosclerotic lesions.we have characterized the expression of allelic variants of x-linked glucose 6-phosphate dehydrogenase (g6pd) in aorta from homozygous, hemizygous, and heterozygous baboons (papio hamadryas). fibrous plaques from heterozygous baboons fed a high cholesterol, saturated fat diet contained distributions of g6pd allelic variants that differed from those of normal arterial wall and fatty streaks. the skewed allelic expression patterns in fibrous plaques of heterozygotes reflect decreased cellular hete ...19938224112
[the effect of psychotropic preparations on the behavioral and hormonal manifestations of emotional stress in monkeys].ethological and radioimmunological techniques were used to examine the effects of psychotropic agents on the behaviour of the male papio hamadryas and the hormonal activity of the steroid-producing glands at physiological rest and during emotional stress. prior (15 minutes before immobilization) intramuscular administration of haloperidol, 0.1 mg/kg, amitriptyline, 2.0 mg/kg or diazepam, 0.76 mg/kg, was demonstrated to produce no stress protective effect, but to result in predominant effects of ...19938220002
the sukhumi primate monkey model for viral lymphomogenesis: high incidence of lymphomas with presence of stlv-i and ebv-like virus.t-cell leukemia virus-like proviral sequences (stlv-i) as well as ebv-like sequences were detected in pbls and tissues of non-human primates (papio hamadryas baboons, green monkeys and macaca arctoides; sukhumi primate center/georgia) by pcr. surprisingly, two different types of stlv-i within papio hamadryas baboons were found. one of its represents the baboon prototype stlv-i-su described earlier, present in lymphomatous baboons from the "high-lymphoma stock", which shows about 83% homology to ...19938395624
corticotropin-releasing hormone in baboon pregnancy.during human pregnancy, plasma crh immunoreactivity (crh-ir) rises progressively, peaking during labor and falling after delivery. among animal species, only higher primates have elevated crh-ir during pregnancy. this study examines whether changes in plasma crh-ir in the baboon (papio hamadryas) are similar to those in the human. crh-ir was determined by ria in 16 baboons at different stages of gestation (44 samples) and in 3 males. assays were performed on vycor extracts of plasma and crh-ir d ...19938473382
[the properties of simian coronavirus].properties of 20 coronavirus (cv) isolates obtained from spontaneously infected papio hamadryas and rhesus monkeys were investigated. two of them were selected as simian cv prototype strains-cvrm 281 (rhesus monkey) and cvp 250 (papio hamadryas), and can be offered as candidates to the coronaviridae family. they are closely related to each other, but differ in some biological properties and polypeptides. these strains belong to the 2nd antigen group of mammalian cv with the prototype strain hcv ...19938073751
nuclear dna polymorphisms in a wild population of yellow baboons (papio hamadryas cynocephalus) from mikumi national park, tanzania.yellow baboons (papio hamadryas cynocephalus) from mikumi national park, tanzania were studied for polymorphisms in nuclear dna. the study population consists of four social groups that inhabit overlapping home ranges and exchange males. as a result, these groups are considered to be members of a single interbreeding population. human dna clones were used as probes to screen five loci (at3, ren, hexb, vim, and apob) for restriction fragment length polymorphisms (rflp). a total of 14 polymorphism ...19938097371
integrating behavior and cardiovascular responses: posture and locomotion. i. static analysis.heart rate, arterial blood pressure, and renal and mesenteric or femoral blood flow were telemetered from 11 papio hamadryas in an untethered free-ranging situation. the animals' behavior was recorded on videotape, and the cardiovascular (cv) data were recorded on the audio channels of the tape. the behavior was coded, and the codes were linked to the cv data via a time-code generator and computer control. the cv data were digitized into 1-s intervals, and the static relations between cv measure ...19938285290
alveolar bone loss in wild baboons.radiograph-based methods were used to assess alveolar bone loss in 12 juvenile and 18 adult baboons (papio hamadryas, sensu lato) living in the awash national park, ethiopia. alveolar bone loss, as measured from the cementoenamel junction to the alveolar crest, averaged about 1 mm in juveniles and about 1.5 mm in adults. densitometry of alveolar bone from the radiographs provides a baseline for comparisons with other adult baboons. periodontal disease surveys of baboon populations using such met ...199331941198
endometriosis and stromal tumor in a baboon (papio hamadryas). 19921318457
inhibitory and excitatory amino acid receptors, c-fos expression, and calcium-binding proteins in the brain of baboons (papio hamadryas) that exhibit 'spontaneous' grand mal epilepsy. 19921363039
x-linked glucose-6-phosphate dehydrogenase (g6pd) and autosomal 6-phosphogluconate dehydrogenase (6pgd) polymorphisms in baboons.electrophoretic polymorphisms of glucose-6-phosphate dehydrogenase (g6pd) and 6-phosphogluconate dehydrogenase (6pgd) were examined in captive colonies of five subspecies of baboons (papio hamadryas). phenotype frequencies and family data verified the x-linked inheritance of the g6pd polymorphism. insufficient family data were available to confirm autosomal inheritance of the 6pgd polymorphism, but the electrophoretic patterns of variant types (putative heterozygotes) suggested the codominant ex ...19921296572
experimental infection of hamadryas baboons papio hamadryas with a human isolate of schistosoma mansoni from saudi arabia. 199217588039
[effects of chronic surfagon administration on secretion of luteinizing hormone and morphofunctional state of the testes in papio hamadryas].the paper is concerned with the results of a study of the effects of chronic (12 weeks) subcutaneous infusion of surfagon (d-ala6, des-gly10-ethylamide of lh-rh, a daily dose of 3 micrograms/kg of body mass) using osmotic mini-pumps on hormonal function of the hypophyseogonadal system, spermatogenesis and testicular histology in experiments on papio hamadryas. prolonged infusion of surfagon through short-term transient activation of the hypophyseogonadal system was shown to cause inhibition of t ...19921308599
[the opsonizing activity of the sera of hamadryas baboons immunized with a preparation of the outer membranes of francisella tularensis (based on data from the luminol-dependent luminescence method)].the opsonizing properties of sera obtained from hamadryas baboons immunized with the preparation of f. tularensis outer membranes (om) were studied with the use of luminol-dependent chemiluminescence (cl) of whole blood. the immunization of monkeys with the om preparation was shown to lead to the formation of functionally active antibodies possessing opsonizing properties with respect to virulent f. tularensis. immune sera obtained from the animals immunized with live vaccine and from those immu ...19921496878
[the comparative action of relanium and oxytocin on higher nervous activity in lower monkeys].effects of relanium and oxytocin on higher nervous activity was studied in four rhesus monkeys (macaca mulatta) and two baboons (papio hamadryas). during observation of the animals in enclosure tranquilizing effect was seen only after relanium administration. under the same conditions oxytocin practically did not change the general behaviour pattern of monkeys. however, steady behavioural transformations were observed under the conditions of competitive food-procuring behaviour and during the op ...19921332292
[spontaneous and experimental hepatitis a in papio hamadryas].data on high susceptibility of papio hamadryas to hav are presented. for the first time, p. hamadryas were shown to be able to respond to both natural and experimental infection developing the features typical of hepatitis a: increased aminotransferase activity, virus shedding in feces, production of anti-hav igg and igm, histological liver lesions. an infection lingering for 3-4 months was observed, as well as a case of chronic experimental hepatitis a with relapse in 7 months of the disease. v ...19921335190
[stress-inducing behavioral changes and the functions of the neurohormonal systems in monkeys of different social ranks].as a result of intensive stressful stimuli hamadryas baboons developed, depending on their hierarchic status, depression-like states varying in severity. their development correlated with a drastic release of biogenic amines, activation of pituitary-adrenal system and inhibition of hormonal function of the gonads. it is shown that dominant and low-rank monkeys, in spite of the differences in their initial psychoemotional states and in the blood levels of mediators and hormones, demonstrated mark ...19921363277
baboon t cell lymphomas expressing the b cell-associated surface proteins cd40 and bgp95.papio hamadryas baboons in the sukhumi colony develop enzootic outbreaks of malignant lymphomas with an incidence of about 1.5% per year among adults of the high-risk stock. we investigated the surface phenotypes of cells from normal and lymphomatous animals using antibodies against human lymphocyte antigens. we found that more than 80% of the lymphomas that developed during the last 3 years were characterized histologically to be of the peripheral t cell type. generally, the lymphomatous cells ...19921383259
detection and characterization of t-cell leukemia virus-like proviral sequences in pbl and tissues of baboons by pcr.the "high lymphoma-prone" baboon stock (papio hamadryas) of the sukhumi primate center colony is characterized by a high prevalence of antibodies to the stlv-i/htlv-i type of retrovirus and a high manifestation of human atl-type (adult t-cell leukemia/lymphoma) malignancies (yakovleva et al., this symposium). this is in contrast to other primate colonies and wild monkeys, which have low seroprevalence and very few if any atl-type t-cell malignancies. to characterize the type of t-cell lymphoma r ...19921318468
[relationship between functional activity of the adrenal glands and gonads in monkeys during puberty].testicular and adrenal cortex endocrine functions were simultaneously tested in pubertal baboons (papio hamadryas) during one year. concentrations of androgens (testosterone, 5 alpha-dihydrotestosterone, dehydroepiandrosterone), corticosteroids (cortisol, progesterone, 17-hydroxyprogesterone, 17-hydroxypregnenolone) and aldosterone were determined in blood of the experimental monkeys by radioimmunoassay. it was shown that with increasing androgen (testosterone and dihydrotestosterone) levels in ...19921421246
a comparative study of natural killer cell activity, lymphoproliferation, and cell phenotypes in nonhuman primates.three different species of nonhuman primates (baboons [papio hamadryas], rhesus monkeys [macaca mulatta], and african green monkeys [cercopithecus aethiops]) were evaluated for their natural killer cell activity, and for the ability of their peripheral blood mononuclear cells to proliferate in response to known mitogens (concanavalin a, phytohemagglutinin, and pokeweed mitogen) and to react with a panel of mouse monoclonal antibodies directed against human leukocyte surface antigens. rhesus monk ...19921557864
immunohistochemical study on the localization of the epitope defined by a human saliva-specific mouse monoclonal antibody (p4-5c).a novel mouse monoclonal antibody (p4-5c) has been developed which recognizes the core portion of the protein carrying abo(h) blood group antigens in human saliva. this proved to be specific for human saliva using immunochemical investigations such as enzyme-linked immunosorbent assay, ouchterlony method and counter-immunoelectrophoresis. by light and electron microscope studies with immunohistochemical techniques using this human saliva-specific p4-5c as primary antibody, it was shown that p4-5 ...19911716968
microscopic anatomy of the baboon (papio hamadryas) adrenal medulla.adrenal medullas of 2 baboons perfused with formaldehyde/glutaraldehyde and tannic acid were studied by light and electron microscopy. tissues were postfixed in oso4. this procedure allows identification of noradrenaline cells on the basis of a selective reaction of glutaraldehyde with noradrenaline vesicles. as positive control for noradrenaline cells, similarly treated mouse adrenal medullas were also examined. light microscopic examination of thick sections of baboon medullas did not show nor ...19911810929
[the use of microdot immunoenzyme analysis with visual detection for the determination of tularemia antibodies].the possibility of using the micropoint enzyme immunoassay (eia) on a nitrocellulose membrane with the visual evaluation of results for the detection of tularemia igg antibodies in hamadryas baboons at the postvaccinal period has been studied. the sensitivity of this assay has been compared with that of the passive hemagglutination (pha) test, the microagglutination (ma) test and eia with the spectrophotometric evaluation of results in plates. as shown in this study, eia in the above-mentioned m ...19911872100
[experimental lassa fever in hamadryas baboons].intramuscular or aerogenic inoculation of baboons with lassa virus reproduced a severe form of the disease which clinically, pathoanatomically and virologically resembles the severe form of human lassa fever reported in the literature. the severity of monkey infection is demonstrated by rapid development of symptoms of general toxicity in the presence of fever, manifestations of hemorrhagic diathesis, high level of viremia and isolation of the causative agent from nasopharyngeal washings.19911882523
[the characteristics of the antibody response of animals immunized with components of the surface structures of francisella tularensis].antibody formation in animals immunized with one of the components of f. tularensis surface structures was studied. the time course of antibody formation in 20 hamadryas baboons was studied in the passive hemagglutination (pha) test, microagglutination (ma) test, and indirect enzyme immunoassay, used for the determination of igg, iga and igm antibodies. the character of antibody response in the animals immunized with components of f. tularensis surface structures (s-complex) and with live tulare ...19911950288
[the postvaccinal reaction in hamadryas baboons to the administration of attenuated strain 15 of the venezuelan equine encephalomyelitis virus].immunization of papio hamadryas with attenuated vee virus (strain 15) causes seroconversion in 100% of animals. postvaccinal reaction such as viremia and short-lasting fever occurs in response to the injection of 10(3) imd50 in about 30% of animals. cns lesion symptoms characterizing infection of papio hamadryas with natural trinidad strain do not occur in response to injection with attenuated strain.19912054507
infectivity of trypanosoma (trypanozoon) brucei gambiense for baboons (papio hamadryas, papio papio).in order to study sensitivity or resistance of t.b. gambiense to baboon serum, two species of baboons, p. hamadryas and p. papio were inoculated with t.b. gambiense clone litat 1.1. both species were receptive to infection but, parasitological and immunological parameters showed that p. papio was more trypanotolerant than p. hamadryas. the vat-specific trypanolysis test and the elisa, using moab for circulating antigen detection may be appropriate for the diagnosis of human trypanosomiasis due t ...19912042999
[effect of corticoliberin and naloxone on the functional activity of the hypophyseal-gonadal system in monkeys].experiments were staged on male papio hamadryas for a study of crf and naloxone action on the time course of lh and testosterone in peripheral blood. lh was determined by a biological micromethod in vitro; acth, cortisol and testosterone were determined by radioimmunoassays. exogenous crf was shown to increase inhibitory action of acute "procedure" stress (frequent fixation of monkeys and venipuncture for taking blood samples) of the secretion of lh but not testosterone. opioid preceptor blockad ...19911658776
variation in lp(a) levels and apo(a) isoform frequencies in five baboon subspecies.elevated levels of lipoprotein (a) [lp(a)] are positively correlated with risk of cardiovascular disease and are thought to be a function of allelic variation in apo(a), the unique protein component of lp(a). in this article we examine subspecies variation in lp(a) levels and apo(a) isoforms in the baboon. breeding populations of the five subspecies (papio hamadryas hamadryas, p.h. cynocephalus, p.h. ursinus, p.h. papio, and p.h. anubis) of common long-tailed baboons are maintained at the southw ...19912004745
linkage of plasminogen (plg) and apolipoprotein(a) (lpa) in baboons.four allelic forms of serum plasminogen (plg) were detected in baboons (papio hamadryas linneaus 1758) by isoelectric focusing and were determined to be inherited as autosomal codominant traits. linkage analysis of data from 179 progeny and their parents revealed that plg is tightly linked (lod score = 30.20) to the gene encoding apolipoprotein(a) (lpa), as in humans. no recombinant individuals were identified. this is the first linkage detected between plg and lpa in any species other than huma ...19911783400
integrating behavior and cardiovascular responses: the code.the next revolution in biology is predicted to be in the integrative domain, and the need to involve physiologists in this kind of research has been recognized. this paper represents an approach to providing some of the tools required for dealing with integrative physiology at the behavioral level. video tape recordings are made of the activities of a group of five baboons (papio hamadryas) while simultaneous recordings of arterial blood pressure, heart rate, renal blood flow, and mesenteric or ...19911858945
cytoarchitecture and intrafrontal connections of the frontal cortex of the brain of the hamadryas baboon (papio hamadryas).a study was made of the cytoarchitecture of the lateral and medial frontal cortex in the hamadryas baboon (papio hamadryas). the frontal cortico-cortical connections of areas 46, 8, 6, and 4 were investigated by injection of wheat-germ agglutinine conjugated to horseradish peroxiase (wga-hrp) into different regions of areas 46, 8, and 6. the lateral region of the frontal lobe of the baboon consists of broad areas of motor (area 4), premotor (area 6), and the dorsolateral prefrontal cortex, each ...19911719042
teeth emergence in wild olive baboons in kenya and formulation of a dental schedule for aging wild baboon populations.teeth emergence schedules are presented from analysis of 95 wild olive baboons papio anubis (age range 2-120 months) and compared to recently published results for wild and captive yellow baboons (p. cynocephalus; phillips-conroy and jolly: american journal of primatology 15:17-29, 1988). age at emergence of m1 (20.5 months males, 19.5 months females), i1 (32.5 and 33.5 months) and i2 (40.0 and 39 months) of olive baboons was earlier than in the wild yellow baboons and similar to captive yellow ...199131952415
genetic differentiation between baboon subspecies: relevance for biomedical research.the use of common names which may encompass a number of subspecies or species is pervasive in the biomedical literature. failure to identify the complete taxonomic classification of research subjects presents a source of error for scientists attempting to evaluate results or to repeat experiments. this paper examines the problem in a common animal model, the baboon. analyses of the genetic distances among five baboon subspecies (papio hamadryas anubis, p.h. cynocephalus, p.h. papio, p.h. ursinus ...199031963994
social relationships of mountain baboons: leadership and affiliation in a non-female-bonded monkey.instead of close and differentiated relationships among adult females, the accepted norm for savanna baboons, groups of drakensberg mountain baboons (papio ursinus) showed strong affiliation of females towards a single male. the same male was usually the decision-making animal in controlling group movements. lactating or pregnant females focused their grooming on this "leader" male, producing a radially patterned sociogram, as in the desert baboon (p. hamadryas); the leader male supported young ...199032075347
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