Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| absence of anisakid larvae in farmed european sea bass (dicentrarchus labrax l.) and gilthead sea bream (sparus aurata l.) in southeast spain. | in the present study, a total of 871 farmed fish, 612 gilthead sea bream (sparus aurata l.) and 259 european sea bass (dicentrarchux labrax l.), were examined for the presence of anisakid larvae. two diagnostic methods were applied, visual inspection and artificial digestion based on the degradation of fish soft tissue in an acidified pepsin enzyme solution. none of the samples examined in this study contained any anisakid parasite. the results suggest that consumption of these farmed fish speci ... | 2010 | 20615348 |
| replacement of dietary fish oil by vegetable oils affects humoral immunity and expression of pro-inflammatory cytokines genes in gilthead sea bream sparus aurata. | commercial gilthead sea bream feeds are highly energetic, fish oil traditionally being the main lipid source. but the decreased fish oil production together with the increased prices of this oil encourages its substitution by vegetable oils, imposing new nutritional habits to aquaculture species. partial replacement of fish oil by vegetable oils in diets for marine species allows good feed utilization and growth but may affect fish health, since imbalances in dietary fatty acids may alter fish i ... | 2010 | 20817101 |
| prolactin-induced production of reactive oxygen species and il-1β in leukocytes from the bony fish gilthead seabream involves jak/stat and nf-κb signaling pathways. | prolactin (prl), a peptide hormone produced by the pituitary gland, was shown to play an important role in the modulation of the immune system of lower and higher vertebrates. to further investigate the effects of prl on the activation of professional phagocytes of bony fish, we stimulated head kidney leukocytes and purified macrophages from the gilthead seabream (sparus aurata l.) with various physiological concentrations of native salmon prl for 2 and 16 h and analyzed the respiratory burst ac ... | 2010 | 20826756 |
| scarcity of parasite assemblages in the adriatic-reared european sea bass (dicentrarchus labrax) and sea bream (sparus aurata). | the shaping forces of parasite community structure still is the main subject in the ecological parasitology whilst community predictability and repeatability showed that hardly a generally applicable role is ever going to be assessed. defining and describing parasite communities can be very useful from the epizootiological point, in order to help in the assessment of the medical and economical impact of certain parasitosis, moreover when hosts are economically valuable species. since parasite as ... | 2010 | 20837380 |
| influence of moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (sparus aurata, l.). | abstract: | 2010 | 20939867 |
| quantitative trait loci for resistance to fish pasteurellosis in gilthead sea bream (sparus aurata). | fish pasteurellosis is a bacterial disease causing important losses in farmed fish, including gilthead sea bream, a teleost fish of great relevance in marine aquaculture. we report in this study a qtl analysis for resistance to fish pasteurellosis in this species. an experimental population of 500 offspring originating from eight sires and six dams in a single mass-spawning event was subjected to a disease challenge with photobacterium damselae subsp. piscicida (phdp), the causative agent of fis ... | 2010 | 20946317 |
| the nutritional background of the host alters the disease course in a fish-myxosporean system. | the aim of the present work was to determine if a practical plant protein-based diet containing vegetable oils (vo) as the major lipid source could alter the disease course when challenged with the myxosporean enteromyxum leei, a wide-spread parasite in the mediterranean basin causing heavy economic losses. gilthead sea bream (sparus aurata) fed for 9 months either a fish oil (fo) diet or a blend of vos at 66% of replacement (66vo diet) were challenged by exposure to parasite-contaminated water ... | 2010 | 20947256 |
| changes in complement responses in gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) under crowding stress, plus viral and bacterial challenges. | gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) were subjected to either experimental infection with photobacterium damselae subsp. piscicida or nodavirus after a period of 2 weeks of crowding in which fish were subjected to a 5-fold increase in density (10-50 kg/m(3)). samples were obtained before the crowding period (0 h or control) and at 24h and 72 h after crowding from both groups of infected fish. the complement haemolytic activity and the expression of the c3 ... | 2010 | 20951810 |
| novel horizontal transmission route for enteromyxum leei (myxozoa) by anal intubation of gilthead sea bream sparus aurata. | the aim of the present study was to determine whether enteromyxum leei, one of the most threatening parasitic diseases in mediterranean fish culture, could be transmitted by peranal intubation in gilthead sea bream sparus aurata l. fish were inoculated either orally or anally with intestinal scrapings of infected fish in 3 trials. oral transmission failed, but the parasite was efficiently and quickly transmitted peranally. prevalence of infection was 100% at 60 d post inoculation (p.i.) in trial ... | 2010 | 21166314 |
| multiple cu-atpase genes are differentially expressed and transcriptionally regulated by cu exposure in sea bream, sparus aurata. | copper (cu) is an essential metal, although in excess is highly toxic due to its redox properties and, therefore intracellular cu homeostasis is a highly regulated process. cu-atpases are pivotal regulatory, proteins of intracellular and bodily cu homeostasis. two cu-atpases, atp7a and atp7b with distinct, functions are found in mammals and herein we report the structure and expression under cu stress of, homologues of atp7a and atp7b in gilthead sea bream (sparus aurata), the first such report ... | 2010 | 20044148 |
| cloning of wap65 in sea bass (dicentrarchus labrax) and sea bream (sparus aurata) and expression in sea bass tissues. | the complementary dna encoding wap65 protein was cloned from the liver of two fish species sea bass (dicentrarchus labrax) and sea bream (sparus aurata). full-length cdna sequences were obtained from reverse transcribed total rna, followed by 5' and 3' rapid amplification of cdna end (race) experiments. the full-length cdna sequence of d. labrax is 1709bp and the coding sequence is flanked by a 67bp 5'-utr and a 358bp 3'-utr. the full-length cdna sequence of s. aurata is 1599bp, and the coding s ... | 2010 | 20085823 |
| coordination of deiodinase and thyroid hormone receptor expression during the larval to juvenile transition in sea bream (sparus aurata, linnaeus). | to test the hypothesis that ths play an important role in the larval to juvenile transition in the marine teleost model, sea bream (sparus auratus), key elements of the thyroid axis were analysed during development. specific rt-pcr and taqman quantitative rt-pcr were established and used to measure sea bream iodothyronine deiodinases and thyroid hormone receptor (tr) genes, respectively. expression of deiodinases genes (d1 and d2) which encode enzymes producing t3, trs and t4 levels start to inc ... | 2010 | 19549532 |
| heavy metal bioaccumulation and metallothionein content in tissues of the sea bream sparus aurata from three different fish farming systems. | the distribution and potential bioaccumulation of dietary and waterborne cadmium and lead in tissues of sea bream (sparus aurata), a major aquaculture species, was studied in relation to three different fish farming systems. metallothionein levels in fish tissues were also evaluated. results demonstrate that metal concentrations in various tissues significantly vary among fish culture systems. different tissues show different capacity for accumulating heavy metals. the content of both cadmium an ... | 2010 | 19444632 |
| metals in tissues of fish from yelkoma lagoon, northeastern mediterranean. | this study was performed to investigate the metal concentrations in muscle, liver, gonad, and gill of gilthead seabream (sparus aurata l., 1758), european seabass (dicentrarchus labrax l., 1758), and keeled mullet (liza carinata valenciennes, 1836) from yelkoma lagoon, northeastern mediterranean region. so, the levels of cadmium, cobalt, chromium, copper, iron, manganese, nickel, lead, and zinc in tissues of specimens from the lagoon were determined by inductively coupled plasma optical emission ... | 2010 | 19680760 |
| anandamide and am251, via water, modulate food intake at central and peripheral level in fish. | the endocannabinoid system is a major regulator of food intake in many animal species. studies conducted so far have mostly focused on mammals, and, therefore, in this study, the role of the endocannabinoid system in food intake in the sea bream sparus aurata was investigated. the effect of different doses of the endocannabinoid anandamide (aea), administered via water, was evaluated after different exposure times (30, 60 and 120 min) at both physiological and molecular levels. the results obtai ... | 2010 | 19800340 |
| investigation of several virulence properties among vibrio alginolyticus strains isolated from diseased cultured fish in tunisia. | we analysed 34 vibrio alginolyticus strains isolated from gilthead sea bream sparus aurata l. and sea bass dicentrarchus labrax l. cultured in fish farms on the tunisian mediterranean coast for the presence of several virulence properties such as extracellular products (ecp) production, growth in iron-limiting conditions and survival in fish serum. the results obtained with different substrates showed that ecp of v. alginolyticus were hydrolytic. the virulence was correlated with the ability of ... | 2009 | 19899346 |
| stimulation of gut immune system by early administration of probiotic strains in dicentrarchus labrax and sparus aurata. | early feeding (started during gut metamorphosis and establishment of the hypothalamic-pituitary-interrenal axis) with probiotic-supplemented diets, besides modifying the intestinal microflora, evoked profound effects on the physiology of fish larvae. using rotifers and artemia as living vectors, the autochthonous bacterium lactobacillus delbrueckii delbrueckii or a multispecies probiotic formulation (autochthonous lactobacillus fructivorans+lactobacillus plantarum from human feces) were orally a ... | 2009 | 19456355 |
| invasion and survival of photobacterium damselae subsp. piscicida in non-phagocytic cells of gilthead sea bream, sparus aurata l. | fluorescence microscopy and gentamicin protection assays were used to investigate the ability of four photobacterium damselae subsp. pisicida (phdp) strains to adhere to and to invade the fish epithelial cell line, saf-1, derived from sparus aurata. all strains tested were detected intracellularly using both techniques, although internalization levels varied among strains. treatment with cytochalasin d and experiments carried out at 4 degrees c demonstrated that a functional host cell cytoskelet ... | 2009 | 19460085 |
| evidence for the involvement of aquaporins in sperm motility activation of the teleost gilthead sea bream (sparus aurata). | the expression of aquaporins in the spermatozoa of the marine teleost gilthead sea bream (sparus aurata) and their involvement in the motility activation process were investigated. sperm motility was activated by a hyperosmotic shock, but it was completely inhibited by 10 microm hgcl(2), such inhibition being partially recovered by beta-mercaptoethanol (me). conventional rt-pcr using primers specific for s. aurata aquaglyceroporin (glp) and aquaporin 1a (aqp1a) demonstrated the presence of both ... | 2009 | 19571262 |
| experimental transmission of sparicotyle chrysophrii (monogenea: polyopisthocotylea) to gilthead seabream (sparus aurata) and histopathology of the infection. | the polyopisthocotylean sparicotyle chrysophrii (van beneden et hesse, 1863) was experimentally transmitted to gilthead seabream (sparus aurata l.) by exposure to eggs (egt) and by cohabitation with naturally parasitized fish (ct). in egt trials, the infection was successfully transmitted by introducing containers with monogenean eggs in the fish tanks, with the highest infection level (85.7% prevalence, 3.3 mean intensity) achieved at 6 weeks post exposure (p.e.) to the infection dose of 650 eg ... | 2009 | 19606789 |
| evaluation of the possible transmission of bse and scrapie to gilthead sea bream (sparus aurata). | in transmissible spongiform encephalopathies (tses), a group of fatal neurodegenerative disorders affecting many species, the key event in disease pathogenesis is the accumulation of an abnormal conformational isoform (prp(sc)) of the host-encoded cellular prion protein (prp(c)). while the precise mechanism of the prp(c) to prp(sc) conversion is not understood, it is clear that host prp(c) expression is a prerequisite for effective infectious prion propagation. although there have been many stud ... | 2009 | 19636413 |
| biochemical response in gilthead seabream (sparus aurata) to in vivo exposure to a mix of selected pahs. | in our previous studies we assessed the biochemical response of juvenile seabream to the exposure to three selected polycyclic aromatic hydrocarbons (pahs) (phenanthrene, pyrene and fluorene) in short-term laboratory experiments. since in the analyses conducted in the field it is much more natural to expect the presence of multiple contaminants at the same time, the objective of this study was to analyse the reaction of seabream to the exposure to a mix of the three pahs tested previously. the p ... | 2009 | 19167757 |
| high potential of adhesion to abiotic and biotic materials in fish aquaculture facility by vibrio alginolyticus strains. | the ability of vibrio alginolyticus strains isolated from sparus aurata and dicentrarchus labrax nursery to adhere to epithelial cell lines (hep-2 and caco-2), fish mucus and their ability to form a biofilm on different surfaces (glass, polystyrene, polyethylene and polyvinyl-chloride) was investigated in this study. | 2009 | 19245411 |
| a yeast assay based on the gilthead sea bream (teleost fish) estrogen receptor beta for monitoring estrogen mimics. | a yeast (saccharomyces cerevisiae)-based assay was developed and tested with steroids and chemicals (mostly pesticides). the induction of beta-galactosidase activity was strictly dependent on the presence of seabream (sparus aurata) betaa estrogen receptor (sberbetaa) and substances known to have estrogenic activity. 17beta-estradiol (e(2)) and diethylstilbestrol (des), both agonists, were most active and the antagonist tamoxifen (tam) was 14-fold less active than e(2). among the chemicals teste ... | 2009 | 19303142 |
| description of enterovibrio nigricans sp. nov., reclassification of vibrio calviensis as enterovibrio calviensis comb. nov. and emended description of the genus enterovibrio thompson et al. 2002. | eleven strains of halophilic, facultative anaerobes isolated from healthy and diseased dentex dentex and sparus aurata (bony fishes) cultured in spanish mediterranean fisheries have been studied by a polyphasic approach that included a wide phenotypic characterization, dna-dna hybridization and phylogenetic analysis using 16s rrna, reca and rpod gene sequences. all strains were phylogenetically related to enterovibrio species and vibrio calviensis. on the basis of sequence analysis and dna-dna h ... | 2009 | 19329591 |
| isolation and characterization of toll-like receptor 9 in half-smooth tongue sole cynoglossus semilaevis. | toll-like receptors (tlrs) are considered as key sensors to trigger the host's innate immune system and adaptive immune responses by recognizing various pamps and initiating signal transduction. tlr9, as a member of tlr family, mediates the recognition of unmethylated cpg dinucleotide motifs commonly found in both bacterial and viral genomes. in the current study, the tlr9 gene was isolated from one of flatfish species, half-smooth tongue sole (cynoglossus semilaevis). in the 4588 bp genomic seq ... | 2009 | 19340952 |
| profiling of infection specific mrna transcripts of the european seabass dicentrarchus labrax. | the european seabass (dicentrarchus labrax), one of the most extensively cultured species in european aquaculture productions, is, along with the gilthead sea bream (sparus aurata), a prospective model species for the perciformes which includes several other commercially important species. massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. revealing transcripts involved in immune response and studying their relative expression enhances the und ... | 2009 | 19361338 |
| some organochlorine pesticide residues in fish species in konya, turkey. | the levels of organochlorine pesticides were measured in 18 fish species from konya markets, turkey. these species were selected on the basis of their importance to local human fish consumption. the extracted residues were analyzed on a micro capillary gas chromatograph equipped with an electron capture detector. total 14 different organochlorine pesticides were determined. these residues were detected in all fish species, except in trout, horse mackerel and bonito. ddt and its metabolites and h ... | 2009 | 19103455 |
| gyrodactylus orecchiae sp. n. (monogenea: gyrodactylidae) from farmed populations of gilthead seabream (sparus aurata) in the adriatic sea. | gyrodactylus orecchiae sp. n. (monogenea, gyrodactylidae) is described from the skin, fins, eyes and gills of juvenile sparus aurata l. (gilthead seabream) following two outbreaks of gyrodactylosis amongst stocks held in inshore floating cages on the adriatic coast of albania and croatia. fish were heavily infected (1000+ gyrodactylids/fish) with g. orecchiae which reportedly resulted in approximately 2-10% mortality amongst the infected stock. morphologically, the haptoral hooks of g. orecchiae ... | 2009 | 19391328 |
| evaluation of candidate reference genes for qpcr during ontogenesis and of immune-relevant tissues of european seabass (dicentrarchus labrax). | the expression level of mrna can vary significantly in different experimental conditions, such as stress, infection, developmental stage or tissue. suitable reference genes are expected to exhibit constant expression levels. however no single gene is constitutively expressed in all cell types and under all experimental conditions. it has become clear that expression stability of the intended reference gene has to be examined before each experiment. for expression studies using quantitative real- ... | 2009 | 19398033 |
| evaluation of trace heavy metal levels of some fish species sold at retail in kayseri, turkey. | cd, ni, cr, zn, cu and pb concentrations of muscle tissue of 61 fish samples belonging to six fish species (sparus auratus, pomatomus saltatrix, sarda sarda, engraulis encrasicholus, sander lucioperca, scomber scombrus) retailed in kayseri, turkey were determined by flame atomic absorption spectrometry after wet digestion. cd concentrations of at least 31 fish samples (50.8%) and pb concentrations of at least six fish samples (9.8%) exceeded the corresponding turkish permissible limit of 0.05 an ... | 2009 | 18344008 |
| seasonal trends and tissue distribution of organochlorine pollutants in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean sea and their relationship with environmental and biological factors. | seasonal trends and tissue distribution of organochlorine pesticides (ocps) and polychlorinated biphenyls (pcbs) in gilthead sea bream (sparus aurata) samples from the western mediterranean were investigated. hexane extracts of white and red muscle, liver, and gills from wild and cultured specimens were purified by normal-phase high-performance liquid chromatography, and selected ocps and pcbs were determined by gas chromatography coupled to mass spectrometry as indicators of contamination. the ... | 2009 | 18779940 |
| application of in situ detection techniques to determine the systemic condition of lymphocystis disease virus infection in cultured gilt-head seabream, sparus aurata l. | immunohistochemistry (ihc) and in situ hybridization (ish) techniques have been used for the detection of lymphocystis disease virus (lcdv) in formalin-fixed, paraffin-embedded tissues from gilt-head seabream, sparus aurata l. diseased and recovered fish from the same population were analysed. ihc was performed with a polyclonal antibody against a 60-kda viral protein. a specific digoxigenin-labelled probe, obtained by pcr amplification of a 270-bp fragment of the gene coding the lcdv major caps ... | 2009 | 18803582 |
| effect of in vitro exposure to vibrio vulnificus on hydroelectrolytic transport and structural changes of sea bream (sparus aurata l.) intestine. | the everted gut sac technique has been used to investigate the effect of vibrio vulnificus on water and electrolyte (na(+), k(+), cl(-), hco(3)(-)) transport on the intestine of sea bream (sparus aurata l.). both the anterior and the posterior intestine were incubated in a medium containing 10(8) v. vulnificus cells ml(-1) at 25 degrees c for 2 h. the presence of v. vulnificus resulted in a significant reduction (p < 0.05) of water absorption in the anterior intestine, while sodium absorption in ... | 2009 | 18825505 |
| viral encephalopathy and retinopathy of dicentrarchus labrax and sparus aurata farmed in tunisia. | viruses belonging to the nodaviridae family cause disease worldwide among a large number of species of marine fish, and have been described in all continents. in the present study, a total of 69 farmed tunisian sea bass (dicentrarchus labrax) and 24 sea bream (sparus aurata) samples were tested monthly for the detection of betanodavirus. the virus was identified in both species using indirect immunofluorescence assays (ifat) and rt-pcr. in addition sequence analysis of part of the coat protein g ... | 2009 | 18982421 |
| lectinhistochemical detection of terminal carbohydrate residues in the enteric myxozoan enteromyxum leei parasitizing gilthead seabream sparus aurata (pisces: teleostei): a study using light and transmission electron microscopy. | the presence of terminal carbohydrate residues in enteromyxum leei (diamant, lom et dyková, 1994) palenzuela, redondo et alvarez-pellitero, 2002 stages in gilthead seabream intestines was studied at light microscopy (lm) and transmission electron microscopy (tem) level using lectin histochemical techniques. abundant mannose and/or glucose residues were demonstrated by the intense staining caused by binding of biotinylated concanavalin a (con a), at both lm and tem. a clear positivity was also ob ... | 2009 | 20128238 |
| comparing the inhibitory effects of five protoxicant organophosphates (azinphos-methyl, parathion-methyl, chlorpyriphos-methyl, methamidophos and diazinon) on the spontaneously beating auricle of sparus aurata: an in vitro study. | organophosphates (ops) can provoke toxicity by inhibiting acetylcholinesterase (ache) in non-target organisms, like fish. in a previous pilot study, the anticholinesterase effects of paraoxon on the heart of sparus aurata were examined [tryfonos, m., antonopoulou, e., papaefthimiou, c., chaleplis, g., theophilidis, g., 2009. an in vitro assay for the assessment of the effects of an organophosphate, paraoxon, and a triazine, atrazine, on the heart of the gilthead sea bream (sparus aurata). pest. ... | 2009 | 19674799 |
| interactive effects of environmental salinity and temperature on metabolic responses of gilthead sea bream sparus aurata. | the gilthead sea bream sparus aurata is a euryhaline and euritherm species with the capacity of living under different environmental conditions of salinity and temperature. the influence of acclimation to different environmental salinities (5, 38 and 55 per thousand) and temperatures (12 degrees , 19 degrees and 26 degrees c) for seven weeks was analyzed in plasma and tissues (liver, gills and kidney) of gilthead sea bream assessing levels of metabolites and enzyme activities related to energy m ... | 2009 | 19651228 |
| pituitary gene and protein expression under experimental variation on salinity and temperature in gilthead sea bream sparus aurata. | temperature and salinity are important factors that affect several physiological processes in aquatic organisms, which could be produced by variation of certain hormones. in this study, the expression of pituitary hormones involved in the acclimation to different temperatures and salinities was examined in sparus aurata, a euryhaline and eurytherm species, by q-real time rt-pcr and western blot analyses for mrna and protein expression, respectively. three different experimental conditions were d ... | 2009 | 19607931 |
| cytochrome p450 (cyp1a) induction in sea bream (sparus aurata) gills and liver following exposure to polychlorobiphenyls (pcbs). | 2009 | 19588260 | |
| targets for tnfalpha-induced lipolysis in gilthead sea bream (sparus aurata l.) adipocytes isolated from lean and fat juvenile fish. | the present study aimed to analyze adiposity heterogeneity and the role of liver x receptor (lxralpha) and peroxisome proliferator-activated receptors (ppars) as targets of tumour necrosis factor-alpha (tnfalpha) in gilthead sea bream (sparus aurata l.). the screening of 20 fish at the beginning of the warm season identified two major groups with fat and lean phenotypes. fat fish showed increased liver and mesenteric fat depots. this increased adiposity was concurrent in the adipose tissue to en ... | 2009 | 19561215 |
| dynamics of liver gh/igf axis and selected stress markers in juvenile gilthead sea bream (sparus aurata) exposed to acute confinement: differential stress response of growth hormone receptors. | the time courses of liver gh/igf axis and selected stress markers were analyzed in juvenile gilthead sea bream (sparus aurata) sampled at zero time and at fixed intervals (1.5, 3, 6, 24, 72 and 120 h) after acute confinement (120 kg/m(3)). fish remained unfed throughout the course of the confinement study, and the fasting-induced increases in plasma growth hormone (gh) levels were partially masked by the gh-stress inhibitory tone. hepatic mrna levels of growth hormone receptor-i (ghr-i) were not ... | 2009 | 19524697 |
| effects of fish oil replacement and re-feeding on the bioaccumulation of organochlorine compounds in gilthead sea bream (sparus aurata l.) of market size. | organochlorine pesticide residues and polychlorinated biphenyls were determined in raw materials, fish feeds and fillets from fish exposed through the productive cycle (14 months) to experimental diets with different percentages of fish oil replacement with vegetable oils. detectable amounts of organochlorine compounds were found in raw materials derived from fish sources with none being detected in vegetable ingredients. fish feeds presented trace concentrations of contaminants at the ng/g leve ... | 2009 | 19450868 |
| effects of feeding schedule on locomotor activity rhythms and stress response in sea bream. | feeding cycles entrain biological rhythms, which enable animals to anticipate feeding times and so maximize food utilization and welfare. in this article, the effect of mealtime was investigated in two groups of sea bream (sparus aurata): one group received a single daily meal at random times during the light period (random daytime feeding, rdf), whereas the other group received the meal during the light period but at the same time (scheduled daytime feeding, sdf). all the fish showed diurnal be ... | 2009 | 19410591 |
| disruption of the thyroid system by diethylstilbestrol and ioxynil in the sea bream (sparus aurata). | some environmental contaminants are thought to cause disruption of the thyroid system in vertebrates acting as endocrine disrupting chemicals (edcs). such chemicals may affect synthesis, transport and metabolism of thyroid hormones (ths). ioxynil (iox) and diethylstilbestrol (des) are potential edcs with strong affinity in vitro for sea bream transthyretin (ttr), a th distributor protein (thdp). the aim of the present study was to establish how such chemicals influence the thyroid axis in sea br ... | 2009 | 19375178 |
| expression of pituitary prolactin, growth hormone and somatolactin is modified in response to different stressors (salinity, crowding and food-deprivation) in gilthead sea bream sparus auratus. | prolactin (prl), growth hormone (gh) and somatolactin (sl) expression was studied in gilthead sea bream (sparus auratus) in response to several different stressors (salinity, food deprivation or stocking density). in the first experiment, specimens were acclimated during 100 days at three different environmental salinities: low salinity water (lsw, 6 ppt), brackish water (bw, 12 ppt) and seawater (sw, 38 ppt). osmoregulatory parameters corresponded to those previously reported for this species u ... | 2009 | 19348804 |
| acute effects of benzo[a]pyrene on liver phase i and ii enzymes, and dna damage on sea bream sparus aurata. | in the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons benzo[a]pyrene (b[a]p) were investigated in the liver of sparus aurata (sea bream). sexually immature gilthead sea bream were treated by intraperitoneal injection of b[a]p (20 mg kg(-1)) for 6, 12, 24, and 48 h. b[a]p accumulation was quantified in sea bream liver by mean of gas phase chromatography (gpc-ms) after the various exposure periods. the following biological res ... | 2009 | 19343524 |
| seasonality of nuclear abnormalities in gilthead sea bream sparus aurata (l.) erythrocytes. | the frequencies of erythrocyte nuclear abnormalities, such as irregularly shaped (isn), vacuolated nuclei (vn), binucleated cells (bn), including micronuclei (mn), were monitored in the peripheral blood of cultured gilthead sea bream (sparus aurata l.) from the southern adriatic sea (croatia) and assessed for a relation to seasonality. fish were sampled in february, april, june, and september. the lowest frequencies of isn and mn were measured in april, and the lowest frequencies of vn and bn we ... | 2009 | 19343523 |
| [strong genetic differentiation of the gilthead sea bream sparus aurata (l., 1758) between the two western banks of the mediterranean]. | we characterised four samples of gilthead sea bream from the two western mediterranean banks with three microsatellite loci and two rapds systems. contrarily to what could be predicted for a highly mobile species with a planktonic larval dispersal phase, we observed a strong and significant genetic differentiation at all loci between the two banks (f(st)=0.069(***)), whereas two samples from the gulf of lions were almost identical (f(st)=0.003 ns) while the two from the gulf of annaba displayed ... | 2009 | 19304263 |
| gene and protein expression for prolactin, growth hormone and somatolactin in sparus aurata: seasonal variations. | the seasonal variation of prl, gh and sl gene and protein expression has been analyzed in gilthead sea bream (sparus aurata) pituitaries using real-time q-pcr and western blots, respectively. animals were cultured in earthen ponds under natural photoperiod, temperature and salinity conditions. samples were taken during winter 2005 (january), spring 2005 (april), summer 2005 (july) and autumn 2005 (october). beta-actin, used as the housekeeping gene both for q-rt-pcr and western analysis, did not ... | 2009 | 19250974 |
| establishment of dominance relationships in gilthead sea bream sparus aurata juveniles during feeding: effects on feeding behaviour, feed utilization and fish health. | in gilthead sea bream sparus aurata held in groups of two, five or 10 fish, social hierarchies were observed. subordinate s. aurata were characterized by elevation of basal levels of plasma cortisol, together with a reduced immunological potential. subordinate fish also showed lower feed intake, feed utilization and lower growth. fatty acid composition was also affected by social status, with a lower content of saturated acids, oleic and eicosapentaenoic, in muscle and liver of fish considered a ... | 2009 | 20735600 |
| comparison among different gilthead sea bream (sparus aurata) farming systems: activity of intestinal and hepatic enzymes and 13c-nmr analysis of lipids. | in order to evaluate differences in general health and nutritional values of gilthead sea bream (sparus aurata), the effects of semi-intensive, land-based tanks and sea-cages intensive rearing systems were investigated, and results compared with captured wild fish. the physiological state was determined by measuring the activity of three different intestinal digestive enzymes: alkaline phosphatase (alp), leucine aminopeptidase (lap) and maltase; and the activity of the hepatic alp. also, the hep ... | 2009 | 22253985 |
| classification accuracy of algorithms for blood chemistry data for three aquaculture-affected marine fish species. | the objective of this study was determination and discrimination of biochemical data among three aquaculture-affected marine fish species (sea bass, dicentrarchus labrax; sea bream, sparus aurata l., and mullet, mugil spp.) based on machine-learning methods. the approach relying on machine-learning methods gives more usable classification solutions and provides better insight into the collected data. so far, these new methods have been applied to the problem of discrimination of blood chemistry ... | 2009 | 19031001 |
| immunohistochemical detection of estrogen receptors in fish scales. | calcium mobilization from internal stores, such as scales, induced by 17beta-estradiol during sexual maturation in salmonids is well documented. this calcium mobilization from scales is proposed to be mediated by the estrogen receptor (er). however, the er subtypes involved and signaling mechanisms responsible for this effect remain to be fully characterized. in the present study, we have localized eralpha, erbetaa and erbetab proteins in juvenile and adult sea bream (sparus auratus) and mozambi ... | 2009 | 18977356 |
| synchronization to light and restricted-feeding schedules of behavioral and humoral daily rhythms in gilthead sea bream (sparus aurata). | food is not continuously available in the wild, and so most animals show a wide variety of circadian rhythms that can be entrained to feeding time. the aim of this research was to evaluate the effect of time-restricted feeding on the daily rhythms of gilthead sea bream, with food being provided during the day or night under a 12:12 h light-dark (ld) cycle or constant light (ll) conditions. self-feeding and locomotor activity, as well as daily rhythms of cortisol, glucose, and melatonin, were eva ... | 2009 | 19916838 |
| evolution of lipopolysaccharide (lps) recognition and signaling: fish tlr4 does not recognize lps and negatively regulates nf-kappab activation. | it has long been established that lower vertebrates, most notably fish and amphibians, are resistant to the toxic effect of lps. furthermore, the lack of a tlr4 ortholog in some fish species and the lack of the essential costimulatory molecules for lps activation via tlr4 (i.e., myeloid differentiation protein 2 (md-2) and cd14) in all the fish genomes and expressed sequence tag databases available led us to hypothesize that the mechanism of lps recognition in fish may be different from that of ... | 2009 | 19201835 |
| leptotheca sparidarum n. sp. (myxosporea: bivalvulida), a parasite from cultured common dentex (dentex dentex l.) and gilthead sea bream (sparus aurata l.) (teleostei: sparidae). | a new myxosporean, leptotheca sparidarum n. sp., is described from the trunk kidney of two sparid fish, sparus aurata and dentex dentex, in several culture facilities from the western mediterranean coasts. it is distinguished from all the previously described species by spore morphometrics. spores with two equal polar capsules and one binucleated sporoplasm. spore measurements from d. dentex were 5-7.1 micron long x 8.8-12.3 micron thick x 5.88-6.18 micron wide; polar capsules 2.6-3.5 micron in ... | 2009 | 11831771 |
| spermiogenic nuclear protein transitions and chromatin condensation. proposal for an ancestral model of nuclear spermiogenesis. | we have chosen three species (sparus aurata, dicentrarchus labrax, and monodonta turbinata) that represent different transition patterns in the composition and structure of spermiogenic nuclei. the transition patterns of these species are representative of spermiogenesis in a large number of animal species. we analyze: (a) nuclear protein exchange; (b) chromatin condensation pattern; and (c) histone acetylation during spermiogenic development. in the simplest spermiogenesis histones and nucleoso ... | 2009 | 19132734 |
| older triploid fish retain impaired reproductive endocrinology in the european sea bass dicentrarchus labrax. | this paper reports on an evaluation of growth, gonadal development and reproductive endocrinology of older triploid (3n) european sea bass dicentrarchus labrax in comparison with their diploid (2n) counterparts throughout their fifth and seventh annual cycle of life. while older triploids retained impaired reproductive endocrinology, a sexually related dimorphic growth was observed with 3n females attaining the largest sizes. comparisons of some body indexes showed that 3n females had a signific ... | 2009 | 20738514 |
| melatonin concentrations during larval and postlarval development of gilthead sea bream sparus auratus: more than a time-keeping molecule? | in this study, melatonin (mel) and thyroxine (t(4)) concentrations were measured during larval and postlarval development of gilthead sea bream sparus auratus hormones were measured in whole bodies of larvae or the head and trunk of postlarvae after 67 days of exposure to constant light, 24l:0d, constant darkness, 0l:24d or 12l:12d and in the plasma of 6 month juveniles kept under the 12l:12d, 0l:24d and 24l:0d regimes. high mel concentrations in larvae suggested a distinct role of mel in early ... | 2009 | 20738488 |
| molecular cloning and characterization of follistatin in the gilthead sea bream, sparus aurata. | follistatin (fst) is an activin-binding protein that neutralizes the activity of activin. fst also binds other members of the transforming growth factor-beta (tgf-beta) superfamily, including myostatin (mstn). we report herein on the isolation and characterization of a full-length cdna sequence predicted to encode fst in a marine fish, the gilthead sea bream sparus aurata. the deduced amino acid sequence of sea bream fst (safst) is highly conserved to the counterpart sequences in other vertebrat ... | 2009 | 18167029 |
| cadmium accumulation and biochemical responses in sparus aurata following sub-lethal cd exposure. | cadmium (cd), a heavy metal with limited biological function, is widely distributed in the aquatic environment as a result of natural and anthropogenic activities. the effect of 4 and 11 days exposure of gilthead sea bream sparus aurata to sub-lethal concentrations of cd was evaluated as levels of cd content and cd-metallothionein (mt) presence in different organs. the possible genotoxic effect was also evaluated in erythrocytes by using the "comet assay", a promising tool for estimating dna dam ... | 2009 | 18538842 |
| rearing temperature enhances hepatic glucokinase but not glucose-6-phosphatase activities in european sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata) juveniles fed with the same level of glucose. | the aim of this work was to elucidate if the previous results observed in hepatic glucokinase (gk) and glucose-6-phosphatase (g6pase) activities in european sea bass and gilthead sea bream are due to temperature per se or to differences in feed intake at different water temperatures. for that purpose triplicate groups of fish (30 g initial body weight) were kept at 18 degrees c or 25 degrees c during two weeks and fed a fixed daily ration of a glucose-free or 20% glucose diet. at the end of the ... | 2008 | 18508397 |
| purification and kinetic characterization of 6-phosphofructo-1-kinase from the liver of gilthead sea bream (sparus aurata). | 6-phosphofructo-1-kinase (pfk) was purified to homogeneity from liver of gilthead sea bream (sparus aurata) and kinetic properties of the enzyme were determined. the native enzyme had an apparent molecular mass of 510 kda and was composed of 86 kda subunits, suggesting homohexameric structure. at ph 7, s. aurata liver pfk (pfkl) showed sigmoidal kinetics for fructose-6-phosphate (fru-6-p) and hyperbolic kinetics for atp. fructose-2,6-bisphosphate (fru-2,6-p2) converted saturation curves for fru- ... | 2008 | 18483063 |
| freshness monitoring of sea bream (sparus aurata) with a potentiometric sensor. | freshness in one of the main quality attributes for fish commercialization and consumption. the traditional method for fish freshness evaluation is sensory analysis. however, instrumental methods such as electrical, texture and colour measurements, image analysis, vis spectroscopy and electronic noses have been widely studied as objective alternatives. each of these methods has advantages and disadvantages, but none of them can be universally proposed for defining and measuring fish freshness. t ... | 2008 | 26059148 |
| molecular mechanisms determining sperm motility initiation in two sparids (sparus aurata and lithognathus mormyrus). | molecular mechanisms involved in sperm motility initiation in two sparids (sparus aurata and lithognathus mormyrus) have been studied. our comparative study demonstrates that osmolality is the key signal in sperm motility activation in both species, whereas k(+) and ca(2+) do not have any role. the straight-line velocity that resulted, however, was significantly different when measured in sperm activated with non-ionic and/or calcium-free solutions with respect to that measured in seawater-activ ... | 2008 | 18417709 |
| high levels of vegetable oils in plant protein-rich diets fed to gilthead sea bream ( sparus aurata l.): growth performance, muscle fatty acid profiles and histological alterations of target tissues. | the feasibility of fish oil (fo) replacement by vegetable oils (vo) was investigated in gilthead sea bream (sparus aurata l.) in a growth trial conducted for the duration of 8 months. four isolipidic and isoproteic diets rich in plant proteins were supplemented with l-lysine (0.55 %) and soya lecithin (1 %). added oil was either fo (control) or a blend of vo, replacing 33 % (33vo diet), 66 % (66vo diet) and 100 % (vo diet) of fo. no detrimental effects on growth performance were found with the p ... | 2008 | 18377678 |
| cyp27a1 expression in gilthead sea bream (sparus auratus, l.): effects of calcitriol and parathyroid hormone-related protein. | little is known about vitamin d metabolism in fishes. several reports have shown hydroxylase activities in various organs to produce vitamin d metabolites, but the enzymes involved have not been isolated or characterized. we isolated and characterized a renal mitochondrial hydroxylase, cyp27a1, that governs vitamin d metabolism in gilthead sea bream, sparus auratus. the enzyme is highly expressed in kidney and to a far lesser extent in liver. when treated with 25-hydroxy vitamin d or calcitriol, ... | 2008 | 18310458 |
| regulatory regions in the promoter and first intron of sparus aurata growth hormone gene: repression of gene activity by a polymorphic minisatellite. | transcriptional activity of the gilthead sea bream (sparus aurata) growth hormone (sagh) gene promoter has been investigated using transient transfection assays in gh3 cells. analysis of two fragments (1.7 kb and a 5'-deleted 0.9 kb) of sagh gene promoter directed luciferase reporter gene activity, indicating transcriptional activity of both fragments in vitro. the shorter fragment, containing five potential binding sites for the pituitary-specific transcription factor ghf-1/pit-1, two camp-resp ... | 2008 | 20483206 |
| copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (sparus aurata) after exposure to dietary or waterborne copper. | the high affinity copper transporter 1 (ctr1), metallothionein (mt) and glutathione reductase (gr) are essential for copper uptake, sequestration and defense respectively. following rearing on a normal commercial diet (12.6+/-0.2 mg kg(-1) cu), sea bream were fed an experimental control diet lacking mineral mix (7.7+/-0.3 mg kg(-1) cu), an experimental diet enhanced with cu (135+/-4 mg kg(-1) cu) or an experimental diet (7.7+/-0.3 mg kg(-1) cu) whilst exposed to cu in water (0.294+/-0.013 mg l(- ... | 2008 | 18304880 |
| investigating the presence of organochlorine pesticides and polychlorinated biphenyls in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean sea. | the presence of organochlorine pesticides and polychlorinated biphenyls in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean, spain, is investigated. these pollutants were determined in white and red muscle and liver by gas chromatography coupled to tandem mass spectrometry. contaminant profiles present in both wild and farmed fish tissues include ddts, pcbs and hcb. the highest concentrations of contaminants were found in liver, and an increase in pollutant level ... | 2008 | 18289607 |
| confinement exposure induces glucose regulated protein 75 (grp75/mortalin/mthsp70/pbp74/hspa9b) in the hepatic tissue of gilthead sea bream (sparus aurata l.). | glucose regulated protein 75 (grp75/mortalin/mthsp70/pbp74/hspa9b) is a molecular chaperone that was partially cloned and sequenced in gilthead sea bream (sparus aurata l.) using a rt-pcr and 3'race approach. the deduced amino acid sequence supported the early vertebrate divergence of the heat shock protein 70 family into cytoplasmic hsp70/hsc70 group, endoplasmic reticulum-resident group and the mitochondrial-type group of grp75. the tissue-specific regulation of grp75 was analyzed by real-time ... | 2008 | 18164226 |
| high density and food deprivation affect arginine vasotocin, isotocin and melatonin in gilthead sea bream (sparus auratus). | arginine vasotocin (avt) and isotocin (it) levels in plasma and pituitary, and melatonin (mel) levels in plasma were determined in gilthead sea bream (sparus auratus) subjected to two different types of stress: i) high density (hd) and ii) food deprivation (nf: non-fed). fishes were randomly assigned to one of 4 treatments that lasted for 14 days: 1) fed fish under normal low density (nd, 4 kg m(-3)); 2) non-fed (nf) fish under nd; 3) fed fish under high density (hd, 70 kg m(-3)); and 4) non-fed ... | 2008 | 18054261 |
| t cell receptor beta chain from sea bream (sparus aurata): molecular cloning, expression and modelling of the complexes with mhc class i. | the t cell receptor is a fundamental mediator of the adaptive immune responses, since tr alphabeta on t cells recognize foreign structures (peptides derived from processed antigens) bound to the major histocompatibility complex (mhc) on apc cells. in the present study, we report the cloning of six trb chains cdna sequences from gilthead sea bream (sparus aurata), a fish of high economical impact in south mediterranean aquaculture. the v-beta domains have the canonical features of known teleost a ... | 2008 | 18035419 |
| hepatic glucokinase and glucose-6-phosphatase responses to dietary glucose and starch in gilthead sea bream (sparus aurata) juveniles reared at two temperatures. | the effects of carbohydrate sources/complexity and rearing temperature on hepatic glucokinase (gk) and glucose-6-phosphatase (g6pase) activities and gene expression were studied in gilthead sea bream juveniles. two isonitrogenous (50% crude protein) and isolipidic (19% crude lipids) diets were formulated to contain 20% waxy maize starch or 20% glucose. triplicate groups of fish (63.5 g initial body weight) were fed each diet to near satiation during four weeks at 18 degrees c or 25 degrees c. gr ... | 2008 | 18023221 |
| biomagnification of organochlorine pollutants in farmed and wild gilthead sea bream (sparus aurata) and stable isotope characterization of the trophic chains. | organochlorine pollutants (pesticides and polychlorinated biphenyls) were analysed in farmed and wild gilthead sea bream (sparus aurata) tissues (white muscle and liver) from the western mediterranean (spain) and in their diets. determination was carried out by gas chromatography coupled to tandem mass spectrometry after clean up of the fatty extracts by normal phase hplc, with detection limits around 0.1 ng/g. carbon and nitrogen stable isotope ratios were also determined in the samples. organo ... | 2008 | 17945331 |
| nodavirus increases the expression of mx and inflammatory cytokines in fish brain. | nodavirus has become a serious pathogen for a wide range of cultured marine fish species. in the present work, the expression of genes related to immune and inflammatory responses of sea bream (sparus aurata l.), considered as non susceptible species, was studied both in vitro and in vivo. no replication of the virus was observed in head kidney macrophages and blood leukocytes. moreover, the enhancement of expression of several immune genes (tumor necrosis factor alpha (tnfalpha), interleukin-1- ... | 2008 | 17543386 |
| changes in cathepsin gene expression and relative enzymatic activity during gilthead sea bream oogenesis. | the aim of this study was to provide evidence on the modulation of lysosomal enzymes in terms of both gene expression and enzymatic activity during follicle maturation. for this purpose three lysosomal enzymes, cathepsins b, d, and l, were studied in relation to yolk formation and degradation, during the main phases of ovarian follicle growth in the pelagophil species, the sea bream sparus aurata. specific attention was focused on the gene expression quantification method, on the assay of enzyma ... | 2008 | 17538957 |
| maternal transfer of organochlorine compounds to oocytes in wild and farmed gilthead sea bream (sparus aurata). | to investigate the transfer of lipophilic organochlorine compounds to the gonads in gilthead sea bream females, liver and oocytes from wild and farmed females, just before spawning, were analysed by gas chromatography coupled to tandem mass spectrometry after clean-up of the fatty extracts by normal phase hplc. this analytical methodology allowed us to achieve limits of detection of around 0.1 ng/g. data obtained show that the chemicals studied are transferred from the liver to oocytes in ratios ... | 2008 | 17720218 |
| role of sp1 and srebp-1a in the insulin-mediated regulation of glucokinase transcription in the liver of gilthead sea bream (sparus aurata). | insulin induction of glucokinase (gck) transcription in the liver is essential for maintaining glucose homeostasis. to study the molecular mechanism underlying the regulation of hepatic gck expression in the carnivorous fish gilthead sea bream (sparus aurata), we analysed the role of sterol regulatory element binding protein-1a (srebp-1a) and specificity protein (sp) 1 in insulin-dependent gck transcription. transient transfection experiments performed in hepg2 cells and electrophoretic mobility ... | 2008 | 17686483 |
| melatonin synthesis under calcium constraint in gilthead sea bream (sparus auratus l.). | brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. access to calcium was limited by diluting the seawater to 2.5 per thousand and removing calcium from the diet or by prolonged feeding of vitamin d-deficient diet. interactions/relations between melatonin and calcium balance and the hypercalcemic endocrines pthrp and calcitriol were assessed. restricting calcium availability in both water and diet had no effect on plasma melatonin, but when cal ... | 2008 | 17434515 |
| linking the genomes of nonmodel teleosts through comparative genomics. | recently the genomes of two more teleost species have been released: the medaka (oryzias latipes), and the three-spined stickleback (gasterosteus aculateus). the rapid developments in genomics of fish species paved the way to new and valuable research in comparative genetics and genomics. with the accumulation of information in model species, the genetic and genomic characterization of nonmodel, but economically important species, is now feasible. furthermore, comparison of low coverage gene map ... | 2008 | 18297360 |
| cellular localization of insulin-like growth factor-ii protein in the sea bass (dicentrarchus labrax) from hatching to adult. | the cellular localization of igf-ii protein was investigated during larval and postlarval developmental stages of sea bass (dicentrarchus labrax) by immunohistochemistry using antisera raised against sparus aurata igf-ii. at hatching, igf-ii immunoreactivity was already present in the skin, developing intestine and skeletal muscle. during larval life igf-ii protein was also observed in heart musculature, in kidney and gill epithelia as well as in liver. in fry skeletal muscle a moderate igf-ii i ... | 2008 | 18283636 |
| growth dynamics of ulva rotundata (chlorophyta) in a fish farm: implications for biomitigation at a large scale(1). | changes in biomass of several macroalgae [ulva rotundata bliding; gracilariopsis longissima (s. g. gmel.) steentoft, l. m. irvine et farnham; ulva intestinalis l.; and cladophora sp.] and marine plants (zostera noltii and ruppia cirrhosa) growing naturally in earthen ponds of a fish farm (acuinova, san fernando, southern spain) were recorded during a year. the farm is mainly devoted to the culture of gilthered seabream (sparus aurata). the most conspicuous algal species thriving in the ponds was ... | 2008 | 27041626 |
| new tetrachromic vof stain (type iii-g.s) for normal and pathological fish tissues. | a new vof type iii-g.s stain was applied to histological sections of different organs and tissues of healthy and pathological larvae, juvenile and adult fish species (solea senegalensis; sparus aurata; diplodus sargo; pagrus auriga; argyrosomus regius and halobatrachus didactylus). in comparison to the original gutiérrez vof stain, more acid dyes of contrasting colours and polychromatic/metachromatic properties were incorporated as essential constituents of the tetrachromic vof stain. this facil ... | 2008 | 15967749 |
| molecular modelling of co-receptor cd8 alpha alpha and its complex with mhc class i and t-cell receptor in sea bream (sparus aurata). | t-cells are the main actors of cell-mediated immune defence; they recognize and respond to peptide antigens associated with mhc class i and class ii molecules. in this paper, we investigated by molecular modelling methods in the teleost sea bream (sparus aurata) the interaction among the molecules of the tertiary complex cd8/mhc-i/tcr, which determines the t-cell-mediated immunological response to foreign molecules. first, we predicted the three-dimensional structure of cd8 alpha alpha dimer and ... | 2008 | 18951040 |
| light synchronization of the daily spawning rhythms of gilthead sea bream (sparus aurata l) kept under different photoperiod and after shifting the ld cycle. | reproduction in most fish is typically a seasonal process, as spawning takes place usually at a given time of the year, depending on the reproduction strategy of the species, to ensure maximal survival of offspring. nevertheless, fish reproduction cannot be considered an exclusively annual phenomenon, because spawning may also show daily rhythmicity. in this study, we investigated the existence of a daily spawning rhythm in gilthead seabream (sparus aurata l) exposed to different light-dark (ld) ... | 2008 | 18780197 |
| effect of cryopreservation on phosphorylation state of proteins involved in sperm motility initiation in sea bream. | we have previously demonstrated that in sea bream sparus aurata motility initiation determined changes in the phosphorylation state of some proteins. this paper describes an investigation of the effect of a freezing-thawing procedure on the protein phosphorylation/dephosphorylation pattern. proteins extracted from fresh and cryopreserved spermatozoa (before and after motility activation) were separated on sds-page, blotted on nitrocellulose membrane and treated with anti-phosphotyrosine, anti-ph ... | 2008 | 18700136 |
| acute effects of cadmium on liver phase i and phase ii enzymes and metallothionein accumulation on sea bream sparus aurata. | this research was designed to study sparus aurata (sea bream) biotransformation and detoxification responses to acute exposure to cadmium (cd). sexually immature gilthead sea bream were treated by intraperitoneal injection of cd chloride (200 microg kg(-1)) for 6, 12, 24 and 48 h. cd accumulation was quantified in sea bream liver by graphite furnace atomic absorption spectroscopy after the various exposure periods. the following biological responses were measured: (1) ethoxyresorufin-o-deethylas ... | 2008 | 18665457 |
| effect of water temperature and dietary starch on growth and metabolic utilization of diets in gilthead sea bream (sparus aurata) juveniles. | we evaluated the effect of dietary starch level on growth performance, feed utilization, whole-body composition and activity of selected key enzymes of intermediary metabolism in gilthead sea bream juveniles reared at 18 and 25 degrees c. a diet was formulated to contain 48% crude protein, 12% lipids and 30% gelatinized maize starch (diet 30gs). two other diets were formulated to include the same level of ingredients as diet 30gs except for the gelatinized starch, which was included at 20% (diet ... | 2008 | 18586542 |
| development and validation of a gene expression oligo microarray for the gilthead sea bream (sparus aurata). | aquaculture represents the most sustainable alternative of seafood supply to substitute for the declining marine fisheries, but severe production bottlenecks remain to be solved. the application of genomic technologies offers much promise to rapidly increase our knowledge on biological processes in farmed species and overcome such bottlenecks. here we present an integrated platform for mrna expression profiling in the gilthead sea bream (sparus aurata), a marine teleost of great importance for a ... | 2008 | 19055773 |
| antibacterial and anti-paf activity of lipid extracts from sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata). | the anti-paf and the antibacterial activities of lipid extracts obtained from cultured sea bass (dicentrarchus labrax) and cultured gilthead sea bream (sparus aurata) were evaluated. total lipids of sea bass and gilthead sea bream exerted paf-like activity while, in higher amounts they inhibited this paf activity. neutral lipids of both sea bass and gilthead sea bream contained only paf antagonists while the polar lipid fractions contained both paf antagonists and agonists. total lipids of sea b ... | 2008 | 26047447 |
| oral delivery of live yeast debaryomyces hansenii modulates the main innate immune parameters and the expression of immune-relevant genes in the gilthead seabream (sparus aurata l.). | microorganisms isolated from fish can be used as prophylactic tools for aquaculture in the form of probiotic preparations. the purpose of this study was to evaluate the effects of dietary administration of the live yeast debaryomyces hansenii cbs 8339 on the gilthead seabream (sparus aurata l.) innate immune responses. seabream were fed control or d. hansenii-supplemented diets (10(6) colony forming units, cfu g(-1)) for 4 weeks. humoral (seric alternative complement and peroxidase activities), ... | 2008 | 19004644 |
| structural and functional divergence of two fish aquaporin-1 water channels following teleost-specific gene duplication. | teleost radiation in the oceans required specific physiological adaptations in eggs and early embryos to survive in the hyper-osmotic seawater. investigating the evolution of aquaporins (aqps) in these vertebrates should help to elucidate how mechanisms for water homeostasis evolved. the marine teleost gilthead sea bream (sparus aurata) has a mammalian aquaporin-1 (aqp1)-related channel, termed aqp1o, with a specialized physiological role in mediating egg hydration. however, teleosts have an add ... | 2008 | 18811940 |
| polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida. | the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ... | 2008 | 18783128 |
| [aquaporins in gametogenesis of vertebrate animals]. | a review of the data on the presence, localization, and supposed role of aquaporin water channels in oocytes of xenopus laevis, oogenesis and maturation of teleosts sparus auratus and oncorhynchus mykiss, oogenesis and oocyte maturation of rats and mice, and spermatogenesis of several mammalians. | 2008 | 18409379 |
| monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead seabream (sparus aurata l.). | the effects of the oral administration of heat-inactivated lactobacillus delbrüeckii ssp. lactis and bacillus subtilis, individually or combined, on gilthead seabream immune responses were investigated both systemically and locally in the gut. in a first experiment, seabream (65 g) were fed for 3 weeks different diets supplemented with 1 x 10(7)cfu g(-1)lactobacillus, 1 x 10(7)cfu g(-1)bacillus, or 0.5 x 10(7)cfu g(-1)lactobacillus plus 0.5 x 10(7)cfu g(-1)bacillus. controls were fed non-supplem ... | 2008 | 18442923 |
| ultrastructure of enteromyxum leei (diamant, lom, & dyková, 1994) (myxozoa), an enteric parasite infecting gilthead sea bream (sparus aurata) and sharpsnout sea bream (diplodus puntazzo). | the ultrastructure of the developmental stages of the myxozoan enteromyxum leei parasitizing gilthead seabream (sparus aurata) intestine and sharpsnout sea bream (diplodus puntazzo) intestine and gallbladder are described. the earliest stage observed was a small dense trophozoite located among enterocytes. proliferative stages, observed intercellularly in the epithelium of the intestine and gallbladder as well as in the lumen, possessed the typical cell-in-cell configuration throughout their dev ... | 2008 | 18460155 |
| effects on growth and biochemical responses in juvenile gilthead seabream 'sparus aurata' after long-term dietary exposure to low levels of dioxins. | the farmed fish gilthead seabream (sparus aurata) were fed with a dry feed spiked with a low level (23 ng who-teq/kg of feed) polychlorinated dibenzo-p-dioxins and dibenzofurans (pcdd/fs) mixture in order to assess bioaccumulation of these contaminants in the muscle and liver tissues after long-term exposure (approximately 390 days). furthermore, effects on fish growth, feeding and on the response of some biochemical markers (induction of the cyp1a dependent erod activity, the conjugating enzyme ... | 2008 | 18472132 |
| molecular characterization of interleukin-6 in the gilthead seabream (sparus aurata). | a cdna clone, designated sbil-6 (seabream interleukin-6), was obtained from a cdna library of enriched immune-stimulated sequences from gilthead seabream. the deduced sbil-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and one n-linked glycosylation site. at the amino acid level sbil-6 shares 23-26% identity with mammalian il-6 sequences and 30-51% identity with other fish il-6 sequences. the structure of the sbil-6 ge ... | 2008 | 18513800 |