Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| metal-specific induction of metallothionein isoforms in the blue crab callinectes sapidus in response to single- and mixed-metal exposure. | metallothioneins (mts) play an important role in the metabolism of copper and zinc during the molt cycle of the blue crab. in this study we examined the hypothesis that mt expression in crabs is metal specific. anion-exchange chromatography showed one major zncumt (znmt-i) in control crabs, two mt isoforms in cadmium-treated crabs (cdmt-i, cdmt-ii), and three forms (cumt-i, cumt-ii, cumt-iii) in copper-treated animals. amino acid analysis of the carboxymethylated apo-mts, purified by reversed-ph ... | 1992 | 1567201 |
| principal component analysis, trace elements, and blue crab shell disease. | 1992 | 1590588 | |
| calcitonin-like immunoreactivity in the blue crab: tissue distribution, variations in the molt cycle, and partial characterization. | calcitonin-like immunoreactivity has been found in blood and tissues of the marine blue crab, callinectes sapidus rathbun. the activity in blood rises significantly during the immediate premolt (d4) stage, but tissue immunoreactivity does not vary significantly with molt stage. the immunoreactivity to antisalmon calcitonin serum is due to a 27.2 kda protein that shows strong similarities in amino acid composition to a similar protein in a lobster and to human calcitonin precursor. the protein is ... | 1992 | 1640198 |
| in vivo magnetic resonance imaging of the blue crab, callinectes sapidus: effect of cadmium accumulation in tissues on proton relaxation properties. | nuclear magnetic resonance imaging (mri) has been used to visualize the internal anatomy of a living blue crab. the resolution obtained in these studies was sufficient to distinguish individual organs by the differences in their proton densities and proton relaxation properties. t1 (spin-lattice relaxation time)-weighted imaging revealed the lipid-rich nature of the hepatopancreas and gonadal tissue. to evaluate the effect of metal-induced stress on the different organs, crabs were exposed to el ... | 1992 | 1645119 |
| bdellovibrios in callinectus sapidus, the blue crab. | bdellovibrios were recovered from the gill tissue of all of 31 crabs sampled and from all samples of epibiota obtained from the ventral shell surface of 15 crabs. the results suggest that the blue crab is a reservoir for bdellovibrios. the association with crabs may be an important factor in the ecology of the bdellovibrios. | 1992 | 16348706 |
| the impact of limb autotomy on mate competition in blue crabs callinectes sapidus rathbun. | this study is the first to demonstrate experimentally that autotomy (self-amputation of a body part) adversely affects competition for mates. experiments were conducted using blue crabs callinectes sapidus rathbun to examine the consequences of limb loss and pairing precedence on mate acquisition by males. two adult males of equivalent size were introduced sequentially into pools containing a sexually-receptive female and observed after 24 h and 48 h. one male in each pair was left intact, while ... | 1992 | 28311879 |
| mercury bioaccumulation in organisms from three puerto rican estuaries. | we analyzed mercury levels in shrimp (palaemonetes sp.), blue crabs (callinectes sp.), fish (tarpon megalops atlantica and tilapia tilapia mossambica), lizards (ameiva exsul), cattle egret (bubulcus ibis) and moorhen (gallinula chloropus) in three estuaries in puerto rico in 1988. there were no quantifiable concentrations greater than the method detection limit of mercury in shrimp, crabs and lizards from any site. mercury levels were also below detection limits in tilapia, except for specimens ... | 1992 | 24226951 |
| the identification and structure-activity relations of a cardioactive fmrfamide-related peptide from the blue crab callinectes sapidus. | the pericardial organs and thoracic ganglia of the blue crab callinectes sapidus were resected and extracted. the extracts were fractionated by hplc and the fractions analyzed by a radioimmunoassay (ria) to fmrfamide. multiple peaks of immunoreactivity were present and one of these, upon fast atom bombardment mass spectrometry (fab-ms) and microsequencing, yielded the sequence gynrsflrfamide. the amount of this peptide in each crab is between 7 and 13 pmol. several incomplete sequences were also ... | 1991 | 1815216 |
| fishing a superfund site: dissonance and risk perception of environmental hazards by fishermen in puerto rico. | risk perception studies show that individuals tend to underestimate significant risks, overestimate negligible ones, and distrust authorities. they also rely on a variety of strategies or heuristics to reach decisions regarding their risk-taking behavior. we report on a survey of fishermen and crabbers engaged in recreational and substance fishing in a puerto rican estuary (near humacao), which has been declared a "superfund site" because of suspected contamination by mercury, and at ecologicall ... | 1991 | 1876726 |
| purification and characterization of an iron-binding protein from the blue crab (callinectes sapidus). | the presence of an iron-binding protein in the hemolymph of the blue crab (callinectes sapidus) was detected by gel filtration of 59fe-labeled hemolymph. the iron-binding protein was purified to homogeneity by ion exchange chromatography. 2. this protein has a mol. wt of 155,000 and consists of a single polypeptide chain with an isoelectric point of 5.0. 3. analysis of the iron-loaded protein indicates that it has a high affinity for iron and the capacity to bind approximately 10 atoms iron/mole ... | 1990 | 2085965 |
| blood glucose changes in the blue crab callinectes sapidus rathbun on transfer from sea water to air. | intertidal decapods regulate their blood glucose with a significant but transitory (60 min) increase upon exposure to atmospheric air, and this has been considered to be an adaptative response related to the almost complete lack of the pasteur effect in facultative anaerobes. in these animals we would not observe an increase in substrate availability to cope with the small amount of energy furnished by anaerobic pathways but rather a general metabolic depression. however, this hypothesis has nev ... | 1990 | 2094547 |
| concurrent protein synthesis is required for in vivo chitin synthesis in postmolt blue crabs. | chitin synthesis in crustaceans involves the deposition of a protein-polysaccharide complex at the apical surface of epithelial cells which secrete the cuticle or exoskeleton. the present study involves an examination of in vivo incorporation of radiolabeled amino acids and amino sugars into the cuticle of postmolt blue crabs, callinectes sapidus. rates of incorporation of both 3h leucine and 3h threonine were linear with respect to time of incubation. incorporation of 3h threonine into the endo ... | 1990 | 2250160 |
| cytokeratin and desmoplakin analogues within an intracellular parasite. | a significant amount of the total protein in the spore sacs of the microsporidian thelohania sp. consisted of the cytoskeletal elements, cytokeratin intermediate filaments, and the desmosomal analogues. the cytokeratin and desmosomal analogues were organized as cage envelopes surrounding the spores within the spore sac stage. thelohania sp. parasitizes the skeletal muscle of callinectes sapidus, a crustacean that does not appear to have cytokeratins or desmosomes. immunoprobe data indicate thelo ... | 1990 | 29314962 |
| what is molt-inhibiting hormone? the role of an ecdysteroidogenesis inhibitor in the crustacean molting cycle. | the in vivo molt-inhibitory effects of the ecdysone biosynthesis inhibitors 3-hydroxy-l-kynurenine and xanthurenic acid were investigated. these ecdysone biosynthesis inhibitors, isolated from the eyestalks of blue crabs (callinectes sapidus), were injected into eyestalk-ablated crayfish (procambarus clarkii). the active factor was found to be species-nonspecific within crabs and crayfish. the seasonal profiles of the xanthurenic acid and ecdysone titers exhibited a staggered relationship. moreo ... | 1989 | 16594067 |
| allostery in callinectes sapidus hemocyanin: cooperative oxygen binding and interactions with l-lactate, calcium, and protons. | allosteric interactions of callinectes sapidus hemocyanin have been investigated by generation of precision oxygen-binding curves using a modified imai apparatus and their subsequent analysis using numerical methods. the monod-wyman-changeux (mwc) model, the mwc model extended to include hybrid r3t3 states, and the phenomenological adair equation were fit to the experimental data. the hybrid mwc model provided a quantitatively better fit to the data than did the mwc model. the fits of the hybrid ... | 1988 | 2837279 |
| comparative properties of channel catfish (ictalurus punctatus) and blue crab (callinectes sapidus) acetylcholinesterases. | 1. acetylcholinesterases (aches) from channel catfish and blue crabs were examined for substrate preference, kms, effects of inhibitors, and ph and osmotic activity profiles. 2. similarities were noted for substrate preference along with ph and osmotic optima. 3. crab ache had a lower km (9 x 10(-5) vs 2 x 10(-4) m) and was more sensitive in terms of ki50s than fish ache to eserine (2.6 x 10(-7) vs 3 x 10(-7) m), malathion (4.5 x 10(-5) vs 1.6 x 10(-4) m) and parathion (6.9 x 10(-5) vs 7 x 10(-4 ... | 1988 | 2905945 |
| occurrence of trematodes in nerves of the blue crab, callinectes sapidus. | 1988 | 3373008 | |
| detection of protein variation in human serum by externally released precipitins from the blue crab, callinectes sapidus. | 1. a number of invertebrate species releases precipitins into the environment. this process is apparently part of an externally directed immunologic system. 2. a saline solution of precipitins released from the blue crab, callinectes sapidus, provided a reagent that produced two different precipitation reactions with proteins from the sera of human individuals. 3. one of the reaction variants was also detected with mammalian antiserum, the other was not. the former variant was associated with a ... | 1987 | 3427930 |
| correlation between osmoregulation and cell volume regulation. | the euryhaline crab, callinectes sapidus, behaves both as an osmoregulator when equilibrated in salines in the range of 800 mosm and below and an osmoconformer when equilibrated in salines above 800 mosm. there exists a close correlation between osmoregulation seen in the whole animal in vivo and cell volume regulation studied in vitro. hyperregulation of the hemolymph osmotic pressure and cell volume regulation both occurred in salines at approximately 800 mosm and below. during long-term equil ... | 1987 | 3565606 |
| the purification and characterization of glutathione s-transferase from the hepatopancreas of the blue crab, callinectes sapidus. | high glutathione s-transferase activity was found in the cytosol of f-cells from the hepatopancreas of the blue crab (callinectes sapidus). purification of glutathione s-transferase from hepatopancreas extracts by sephadex g-200, deae-sephacel, and chromatofocusing resulted in the isolation of two isozymes with isoelectric points of 5.9 and 5.7, as determined by analytical isoelectric focusing. using 1-chloro-2,4-dinitrobenzene as the substrate the specific activities of the two purified isozyme ... | 1987 | 3592675 |
| the effect of dibutyryl camp on sodium uptake by isolated perfused gills of callinectes sapidus. | hemolymph, collected from 25 and 100% seawater acclimated callinectes sapidus and perfused through the isolated gill, causes increases in na+ uptake in the gill. similarly, dibutyryl camp causes increases in na+ uptake when perfused through the gill. since pericardial organ (po) extracts dopamine and octopamine are known to increase camp levels in the perfused gill, it is suggested that these substances from the po may be the hemolymph factors which increase na+ uptake via the mediation of camp ... | 1987 | 3028905 |
| ammonium ion substitutes for k+ in atp-dependent na+ transport by basolateral membrane vesicles. | ion-transporting cells from posterior gills of blue crabs (callinectes sapidus) acclimated to low salinity were used as starting material for the preparation of microsomal membrane vesicles by density gradient centrifugation. the na+-k+-adenosinetriphosphatase (atpase)-enriched basolateral vesicles were loaded with kcl- or nh+4-containing medium by dilution and centrifugation, and initial rates of 22na+ uptake into the vesicles were measured by a rapid filtration procedure. varying the extravesi ... | 1987 | 3030142 |
| quaternary ammonium sulfanilamide: a membrane-impermeant carbonic anhydrase inhibitor. | a novel carbonic anhydrase (ca) inhibitor, quaternary ammonium sulfanilamide (qas), was tested for potency as a ca inhibitor and for its ability to be excluded from permeating biological membranes. inhibitor titration plots of qas vs. pure bovine ca ii and ca from the gills of the blue crab, callinectes sapidus, yielded ki values of approximately 15 microm; thus qas is a relatively weak but effective ca inhibitor. permeability of the qas was directly tested by two independent methods. the inhibi ... | 1987 | 3107405 |
| membrane-associated carbonic anhydrase in gills of the blue crab, callinectes sapidus. | the presence of the enzyme carbonic anhydrase (ca) on the basal membrane of the branchial endothelial cells in the blue crab and its physiological significance were studied in vivo using a membrane-impermeant ca inhibitor, quaternary ammonium sulfanilamide (qas). injection of qas into the hemolymph of callinectes sapidus resulted in the rapid development of a respiratory acidosis; pco2 rose almost 2 torr, ph was lowered by approximately 0.25 units, and total co2 rose by 2 mm. these results suppo ... | 1987 | 3107406 |
| total mercury and its distribution in blue crab and deep water pink shrimp from alexandria coast. | total mercury content and its distribution in muscles and viscera of male and female blue crab (callinectes sapidus rothbum) and deep water pink shrimp (parapenacus longirostris) collected from the 3 main fishing grounds near the alexandria coast in the mediterranean sea was estimated. the results indicate that the mercury content in the muscles of both species differ according to fishing areas, size, sex, and species. more than 75% of total mercury were accumulated in the viscera of both specie ... | 1987 | 3696196 |
| morphological and behavioral antipredatory adaptations of decapod zoeae. | zoeae of some species of estuarine decapods are retained in the estuary throughout development while others are exported into nearshore coastal waters. the horizontal migrations of decapod zoeae to coastal waters may have evolved to reduce the probability of encountering planktivorous fishes which are most abundant in the estuary. if so, then the morphological vulnerability of zoeae to fish predation should be inversely related to the number of predators occurring where they develop. six species ... | 1987 | 28311521 |
| responses to reversed nh3 and nh4+ gradients in a teleost (ictalurus punctatus), an elasmobranch (raja erinacea), and a crustacean (callinectes sapidus):evidence for nh4+/h+ exchange in the teleost and the elasmobranch. | ammonia excretion rates of channel catfish, ictalurus punctatus, little skate (raja erinacea), and blue crab (callinectes sapidus) were measured in experimental regimes which permitted simultaneous assessment of the partial pressure gradients for nonionized nh3 and the chemical concentration gradients of nh4+. under conditions of low external ammonia, the average ammonia excretion was +295 microm kg-1 h-1 for catfish, +149 microm kg-1 h-1 for blue crabs, and +59 microm kg-1 h1 for skates with pa ... | 1986 | 3746231 |
| the uptake and in vivo metabolism of the organophosphate insecticide fenitrothion by the blue crab, callinectes sapidus. | callinectes were exposed to [14c]fenitrothion at a level of 5.2 micrograms/liter in either 22 degrees c, 34 ppt; 22 degrees c, 17 ppt; or 17 degrees c, 34 ppt seawater. uptake from the water, as measured by a decrease in fenitrothion, and the distribution of radioactivity throughout the callinectes were determined. the nature of radiolabeled metabolites in the water and hepatopancreas was also determined. fenitrothion was absorbed more rapidly from the water at the higher salinity and temperatur ... | 1986 | 3764904 |
| colonization of the gut of the blue crab (callinectes sapidus) by vibrio cholerae. | attachment of vibrio cholerae to the mucosal surface of the intestine is considered to be an important virulence characteristic. vibrio cholerae, an autochthonous member of brackish water and estuarine bacterial communities, also attaches to crustacea, a significant factor in multiplication and survival of v. cholerae in nature. the ability of v. cholerae to attach to the gut wall of the blue crab (callinectes sapidus) was examined, and attachment was observed only in the hindgut and not the mid ... | 1986 | 3767362 |
| heterogeneous humoral and hemocyte-associated lectins with n-acylaminosugar specificities from the blue crab, callinectes sapidus rathbun. | callinectes sapidus serum and hemocyte microsomal fraction agglutinated a panel of untreated and enzyme treated vertebrate erythrocytes and cultured lymphoid cell lines. crossed absorption experiments suggested the presence of multiple specific lectins in the serum. the microsomal fraction showed a 35-fold increase in specific activity when compared to the hemocyte lysate suggesting that hemocyte lectins are membrane-associated. agglutination by serum and hemocyte lectins was inhibited by low co ... | 1986 | 3769455 |
| nutritional availability to rats of selenium in four seafoods: crab (callinectes sapidus), oyster (crassostrea virginica), shrimp (penaeus duorarum) and baltic herring (clupea harengus). | 1. the present study was conducted to determine the biological availability to rats of the selenium in four high-se seafoods: crab (callinectes sapidus), oyster (crassostrea virginica), shrimp (penaeus duorarum) and baltic herring (clupea harengus). 2. weanling male rats were fed on a se-deficient torula yeast diet for 4 weeks followed by either continued depletion or repletion for 4 weeks with 0.05, 0.1 or 0.2 microgram se as selenite/g, or 0.1 or 0.2 microgram se as freeze-dried cooked test fo ... | 1986 | 3676154 |
| the effects of temperature, salinity and a simulated tidal cycle on the toxicity of fenitrothion to callinectes sapidus. | the 96 hr lc50 for callinectes sapidus exposed to fenitrothion at 22 degrees c and a salinity of 34 ppt (parts per thousand) was estimated to be 8.6 micrograms/l with a 95% confidence interval of 7.4-9.9 micrograms/l. acute toxicity was shown to increase with increasing temperature as well as increasing salinity. exposure of callinectes to a simulated tidal cycle of 17 ppt salinity change at 6 hr intervals increased the acute toxicity of fenitrothion to callinectes. the autotomization response i ... | 1985 | 2858335 |
| source of vibrio spp. found in the hemolymph of the blue crab, callinectes sapidus. | 1985 | 4031533 | |
| incidence of bacteremia in stressed and unstressed populations of the blue crab, callinectes sapidus. | the incidence of bacteremia in the blue crab, callinectes sapidus, is reported to be in excess of 80%. because these results have been controversial, a field study was initiated to determine the effect of commercial capture and handling stresses on the incidence and levels of infection in blue crabs. the majority (75%) of "unstressed" crabs which were captured individually and bled immediately upon removal from the water were bacteremic, with a geometric mean level of infection of 14 cfu/ml of h ... | 1985 | 4051486 |
| bicarbonate-chloride exchange in gill plasma membranes of blue crab. | the uptake of chloride was studied in gill plasma membrane vesicles from low-salinity-adapted blue crabs (callinectes sapidus). cl- uptake was not na+ dependent. however, when a hco-3 gradient (in greater than out) was imposed across the membrane, a transient overshoot of about 2.5-fold was produced. approximately 90% of the cl- uptake reflected entry into the osmotically active intravesicular space. cl- itself, nitrate, hydroxyl, and sulfite could substitute for hco-3. the hco-3/cl- exchange pr ... | 1985 | 4061678 |
| method for measuring microbial degradation and mineralization of(14)c-labeled chitin obtained from the blue crab,callinectes sapidus. | a method for measuring microbial degradation and mineralization of radiolabeled native chitin is described.(14)c-labeled chitin was synthesized in vivo by injecting shed blue crabs (callinectes sapidus) with n-acetyl-d-[1-(14)c]-glucosamine and allowing for its incorporation into the exoskeleton. the cuticle had a total organic carbon content of 0.48 mg c mg(-1) with a specific radioactivity of 6,356 cpm mg(-1). glucosamine, i.e., chitin content as determined colorimetrically, was 22% (w/w). mic ... | 1985 | 24221359 |
| characterization of a sex pheromone in the blue crab,callinectes sapidus: crustecdysone studies. | the molting hormone/sex pheromone hypothesis of kittredge and takahashi (1972) and kittredge et al. (1971) was explored inc. sapidus. two concentrations of crustecdysone (5 × 10(-5) m and 5 × 10(-6) m) were presented to male crabs in a bioassay system in which courtship behavior was monitored. the results demonstrate that crustecdysone does not stimulate courtship in this species. the physical properties of crustecdysone were also compared to those of the partially purified sex pheromone derived ... | 1984 | 24318783 |
| allosteric modulation of callinectes sapidus hemocyanin by binding of l-lactate. | hemocyanin of the blue crab callinectes sapidus has the typical structure of crustacean hemocyanins in that its smallest in vivo structure is a hexamer of subunits each having a molecular mass of approximately 75 000. as found in the blood, callinectes hemocyanin consists of a mixture of hexamers and dodecamers (typically 1:4). as in other crustacean hemocyanins, the affinity with which oxygen binds to the binuclear copper site has been reported to be very sensitive to ph and to a variety of ino ... | 1984 | 25856833 |
| trace metal residues in shellfish from maryland waters, 1976-1980. | levels of seven heavy metal residues, arsenic, cadmium, chromium, copper, lead, mercury and zinc were monitored in samples of the american oyster (crassostrea virginica), the soft shell clam (mya arenaria), the hard shell clam (mercinaria mercinaria) and the blue crab (callinectes sapidus). samples were taken from the maryland section of the chesapeake bay and its tributaries over a five year period (1976-80). this study was undertaken to provide an estimate of a baseline for values of trace hea ... | 1984 | 6501792 |
| organochlorine residues in shellfish from maryland waters, 1976-1980. | shellfish samples, including the american oyster (craessostrea virginica), the soft shell clam (mya arenaria), the hard shell clam (mercenaria mercenaria) and the blue crab (callinectes sapidus), were taken from the maryland section of the chesapeake bay and its tributaries over a five year period (1976-80) and analyzed to determine residue levels of various organochlorine pesticides. qualitative and quantitative information was obtained for a variety of polychlorinated hydrocarbons. the purpose ... | 1984 | 6501793 |
| acid-base regulation during exercise and recovery in the blue crab, callinectes sapidus. | during swimming activity callinectes sapidus incurred a severe hemolymph acidosis due to elevated levels of pco2 and lactate. although hemolymph lactate concentration rose steadily during 1 h of exercise, hemolymph ph was maximally depressed within the first 15 min. a discrepancy between the quantities of lactate and h+ released from the tissues into the hemolymph is explained by a large apparent efflux of h+ into the ambient seawater, presumably via a branchial ion exchange process. ammonia exc ... | 1984 | 6528111 |
| microsomal cytochromes p-450 from marine crabs. | cytochromes p-450 were isolated from the hepatopancreas of several species of crabs (libinia emarginata, callinectes sapidus, menippe mercenaria and uca minax). elution profiles from an ion exchange column showed similar profiles for all species, indicating one broad cytochrome p-450 peak eluting at 0.2 m k cl. hydroxyapatite chromatography resolved the p-450 into three forms (cytochromes p-450i, p-450ii and p-450iii). the forms of cytochrome p-450 in the different crabs were compared with respe ... | 1984 | 6149863 |
| recognition of xenogeneic proteins by the blue crab: dissociation of the clearance and degradation reactions and lack of involvement of circulating hemocytes and humoral factors. | blue crabs clear foreign proteins from the circulation to the gills in a manner that can be explained by postulating the participation of a finite number of naturally occurring quasi-specific receptors. the rate of this clearance reaction is not increased by previous exposure to the foreign protein although recovery to the normal rate of clearance after high level blockade is quite rapid. the cleared foreign proteins are degraded in the gills in a relatively nonspecific fashion suggestive of a c ... | 1984 | 6724091 |
| total lipid and cholesterol content in the blue crab, callinectes sapidus rathbun. | total lipid and cholesterol concentrations in hepatopancreas, gonad and muscle were analyzed for 70 blue crabs. the concentrations were highest in hepatopancreas, then gonad, and muscle was the lowest. in male crabs, the concentrations decreased from the immature stage (feeding and growth stage) to the mature stage (breeding stage), and from august to september (breeding period) in mature males. in female crabs, the concentrations were comparatively high for peelers (pre-molting stage) and matur ... | 1984 | 6744830 |
| the intracellular partitioning of trace metals in marine shellfish. | marine organisms have evolved a number of metabolic strategies to deal with potentially toxic trace metals. to determine how "detoxification" mechanisms such as metal-binding proteins and concretions are involved in the intracellular partitioning of trace metals in marine shellfish, the oyster, crassostrea virginica, and the blue crab, callincetes sapidus, were exposed to controlled levels of trace metals. oysters accumulated cadmium readily and produced specific low molecular weight, cadmium-bi ... | 1983 | 6879150 |
| concentration of rotavirus and enteroviruses from blue crabs (callinectes sapidus). | a simple method for concentration and detection of rotavirus and enteroviruses in the blue crab is described. virus was separated from tissue homogenates at ph 9.5, concentrated by adsorption to protein precipitates at ph 3.5, and recovered by elution of precipitates at ph 9.2. test samples of 12 to 15 ml were produced from an initial 100 g of crab tissues. cat-floc precipitation was used to remove sample toxicity for cell cultures. recovery effectiveness averaged 52% with poliovirus 1, echoviru ... | 1983 | 6318667 |
| metal ion interactions with limulus polyphemus and callinectes sapidus hemocyanins: stoichiometry and structural and functional consequences of calcium(ii), cadmium(ii), zinc(ii), and mercury(ii) binding. | hemocyanins are oligomeric metalloproteins containing binuclear copper centers that reversibly combine with oxygen molecules. the structural stability and functional properties of these proteins are modified by divalent cations. equilibrium dialysis was used to study the reversible interaction of callinectes sapidus and limulus polyphemus hemocyanins with the divalent cations calcium, cadmium, zinc, copper, and mercury. the number of binding sites and association constants for each cation were o ... | 1983 | 6626526 |
| distribution ofvibrio cholerae in two florida estuaries. | the distribution ofvibrio cholerae was examined in 2 florida estuaries, apalachicola and tampa bay.vibrio cholerae serotype non-01 was the most abundant serotype, being isolated from 45% of the oyster samples, 30% of the sediments, 50% of the waters, and 75% of the blue crabs.vibrio cholerae serotype 01 was isolated from only one oyster sample. strong linear correlations betweenv. cholerae and temperature, salinity, or the other physical/chemical parameters measured,escherichia coli, or fecal co ... | 1983 | 24221617 |
| salinity adaptation of hco3--dependent atpase activity in the gills of blue crab (callinectes sapidus). | a bicarbonate-dependent atpase (ec 3.6.1.3) was found in microsomal preparations from blue crab gills. when the crabs were transferred to low salinity (200 mosmolal) from seawater (1000 mosmolal), the hco3- dependent atpase increased in all gill pairs, reaching its new steady state in 2 weeks. the greatest increase occurred in the sixth and seventh gill pairs (approx. 2.5-fold). maximal enzyme activity was observed at an mg2+ concentration of 2 mm and an optimal ph of 7.8. the apparent ka for hc ... | 1982 | 6125214 |
| specificity for tail fibers of a humoral crab factor neutralizing t2 bacteriophage. | it is now becoming clear that invertebrates, animals lacking the ability to make humoral antibodies, are capable of recognizing self from non-self. in several instances this recognition is manifest in normal ("unimmunized") animals by a rapid clearance of certain foreign materials from the circulation one example of such a rapid innate reaction involves the clearance of t2 bacteriophage from the blue crab (1). other studies have indicated that blue crab plasma contained a factor capable of neutr ... | 1982 | 7067888 |
| heavy metal ion interactions with callinectes sapidus hemocyanin: structural and functional changes induced by a variety of heavy metal ions. | hemocyanins are oligomeric proteins that reversibly bind oxygen. the oxygen binding site is a binuclear copper center bound to the protein by amino acid side chains. the hemocyanin of the blue crab, callinectes sapidus, occurs in vivo as a mixture of 25s dodecamers and 16s hexamers, whose oxygen binding properties are identical. four heavy metals have been used as probes of structure and function in this hemocyanin system. divalent cations of cadmium, copper, mercury, and zinc induced an indefin ... | 1982 | 7093201 |
| incidence of vibrio species associated with blue crabs (callinectes sapidus) collected from galveston bay, texas. | bacteria were readily isolated from the hemolymph of a majority (88%) of the blue crabs collected from galveston bay, texas. the hemolymph of most crabs contained moderate (greater than 10(3) bacteria/ml) to heavy (greater than (10(5) bacteria/ml) infections. large variances were observed in the bacterial number associated with individual crabs, but no significant difference was observed between the mean bacterial levels in the hemolymph of crabs collected during different seasons of the samplin ... | 1982 | 7103475 |
| cadmium-binding proteins from blue crabs (callinectes sapidus) environmentally exposed to cadmium. | 1982 | 7106072 | |
| diurnal changes in compound eye fine structure in the blue crab callinectes. 1. differences between noon and midnight retinas on an ld 11:13 cycle. | 1982 | 7200529 | |
| subunit structure and dissociation of callinectes sapidus hemocyanin. | the hemocyanin of the blue crab, callinectes sapidus has two major components sedimenting with approximate sedimentation coefficients of 17 s and 25 s. molecular weight data based on light scattering and sedimentation equilibrium measurements at ph 7.8 suggest that the two components have molecular weights of approximately 450 000 and 900 000 in the presence of stabilizing ca2+. in the absence of ca2+, the molecular weights are found to be about 5% lower, suggesting some dissociation of the hemo ... | 1981 | 7213799 |
| toxicity of ozonated estuarine water to juvenile blue crabs (callinectes sapidus) and juvenile atlantic menhaden (brevoortia tyrannus). | 1981 | 7248539 | |
| effect of the amino acid histidine on the uptake of cadmium from the digestive system of the blue crab, callinectes sapidus. | 1981 | 7296034 | |
| amiloride-sensitive ammonium and sodium ion transport in the blue crab. | in the estuarine crab, callinectes sapidus, net nh4+ efflux was twice as high in animals acclimated to 17% salinity seawater (sw) (0.495 +/- 0.084 mumol . h-1 . g wet wt-1, n = 7) than in animals acclimated to full-strength 35% sw (0.212 +/- 0.028 mumol . h-1 . g-1, n = 7). amiloride (3 x 10(-4) m) in the external sw reversibly inhibited these effluxes by 63 +/- 6% (n = 6) and 67 +/- 6% (n = 5), respectively. unidirectional na+ influx was reversibly inhibited 42 +/- 6% (n = 7) by amiloride in 17 ... | 1981 | 7304782 |
| uptake and survival of enteric viruses in the blue crab, callinectes sapidus. | uptake of poliovirus 1 by the blue crab, callinectes sapidus, was measured to assess the likelihood of contamination by human enteric viruses. virus was found in all parts of the crab within 2 h after the crab was placed in contaminated artificial seawater. the highest concentrations of virus were found in the hemolymph and digestive tract, but the meat also contained virus. the concentration of virus in the crabs was generally less than in the surrounding water. changes in salinity did not subs ... | 1981 | 6261683 |
| calcium and the tonic release of transmitter at a non-impulsive synapse in the crab. | depolarization-transmitter release coupling was studied in the promotor stretch receptor/motoneuron synapse of the crab. callinectes sapidus, a preparation in which presynaptic action potentials do not occur. intracellular microelectrode recordings were made from the presynaptic terminal and from the somata of postsynaptic motoneurons while injecting current pulses into the peripheral stretch receptor dendrite with the aid of the sucrose-gap. 1. for short current pulses, the relationship between ... | 1980 | 6109020 |
| evaluation of millipore coli-count samplers for monitoring fecal coliforms in the blue crab, callinectes sapidus. | analyses of crab meat homogenates with coli-count samplers (millipore corp.) resulted in fecal coliform counts comparable to counts obtained from standard most-probable-number testing procedures. | 1979 | 391154 |
| changes in ecdysteroids during embryogenesis of the blue crab, callinectes sapidus rathbun. | 1979 | 437355 | |
| the influence of salinity on toxicity of cadmium and chromium to the blue crab, callinectes sapidus. | 1979 | 444712 | |
| transverse sarcomere splitting. a possible means of longitudinal growth in crab muscles. | transversely split sarcomeres are seen in mouthpart muscles of the blue crab in the electron microscope. sarcomeres split only at the h zone. two new sarcomeres are formed by a z disk which appears in the h zone of the splitting sarcomere. splitting may involve breaking of the thick filaments in the h zone, elongation of these filaments, and formation of both new actin filaments and z-disk materials, sarcomere splitting would allow longitudinal growth of muscle cells without lengthening of sarco ... | 1979 | 457766 |
| effects of mercuric chloride on the resting membrane potentials of blue crab (callinectes sapidus) muscle fibers. | the effects of concentrations of mercuric chloride ranging from 3.68 to 368 microm (1--100 ppm) on the resting membrane potentials of muscle fibers in the extensor muscle of the carpopodite in walking legs of adults blue crabs were investigated in vitro. glass micropipettes filled with saturated solutions of potassium chloride and conventional electronic equipment were used. resting membrane potentials in uncontaminated artificial sea water averaged -78 mv +/- 4 mv (s.e.m.). mercuric chloride ad ... | 1979 | 473223 |
| chlorine, temperature, and exposure duration effects of power plant effluents on juvenile blue crabs callinectes sapidus and grass shrimp palaemonetes pugio. | 1979 | 490683 | |
| ponasterone a: a new ecdysteroid from the embryos and serum of brachyuran crustaceans. | the apolar ecdysteroid present in the developing embryo of the blue crab, callinectes sapidus rathbun, is tentatively identified as ponasterone a (2 beta, 14 alpha, 20,22-pentahydroxy-5, beta-cholest-7-en-6-one) on the basis of chromatographic, immunological, and mass spectral evidence. the apolar ecdysteroid present in the serum of land crabs, gecarcinus lateralis, in the late premolt stages of the intermolt cycle is also tentatively identified as ponasterone a on the basis of chromatographic a ... | 1979 | 538780 |
| identification of species in cooked crabmeat by thin layer isoelectric focusing. | thin layer polyacrylamide gel isoelectric focusing (tlief) is described for characterizing the species-specific, heat-denatured proteins of 8 species of crab: red (geryon quinquedens), rock (cancer irroratus), jonah (cancer borealis), blue (callinectes sapidus), king (paralithodes camtschatica), snow (chionoectes spp.), european edible (cancer pagurus), and dungeness (cancer magister). protein pattern differences are shown not only among species, but also between 2 modes of heat processing of th ... | 1979 | 39059 |
| the importance of predation and competition in organizing the intertidal epifaunal communities of barnegat inlet, new jersey. | community organization was studied by experiment and observation from october 1972-october 1974 in the marine epifaunal assemblages at each end of barnegat inlet, new jersey. the rock jetty at the wave-exposed eastern end of the inlet possesses an intertidal community with the following attributes: (1) a high intertidal zone dominated by the barnacle, balanus balanoides, but also occupied by the blue mussel, mytilus edulis, in rock crevices, (2) a mid and low intertidal zone with usually <10% fr ... | 1979 | 28309374 |
| the effects of pentachlorophenol (pcp) and 2,4-dinitrophenol (dnp) on the oxygen consumption of tissues from the blue crab, callinectes sapidus, under different osmotic conditions. | 1978 | 28895 | |
| characterization of glutathione s-transferase activity in tissues of the blue crab, callinectes sapidus. | 1978 | 30574 | |
| temperature and blood acid-base status in the blue crab, callinectes sapidus. | the blood acid-base status of blue crabs. callinectes sapidus, was assessed at temperatures ranging from 10 to 30 degrees c. blood ph varied inversely with temperature, with a slope (dph/tt) of - 0.012. the paco rose only slightly between 10 and 27 degrees c, from 2.09 torr to 2.91 torr, with the result that the total dissolved co2 actually fell slightly due to the decrease in solubility. in vitro the blood behaved like a conventional rosenthal system, with a rise in pco2 at constant ct which wa ... | 1978 | 33428 |
| nonhomogeneous excitatory synapses of a crab stomach muscle. | neuromuscular synapses of pyloric muscle p1 in the blue crab callinectes sapidus were examined using electrophysiological and electron microscopic methods. the muscle is innervated by a single excitatory axon of the stomatogastric ganglion. excitatory postsynaptic potentials show striking facilitation at very low frequencies of stimulation, indicating very slow decay of the facilitation process after a single nerve impulse. quantal content of transmitter release at a low frequency of stimulation ... | 1978 | 632814 |
| an evaluation of acute bromine chloride toxicity to grass shrimp (palaemonetes spp.) and juvenile blue crabs (callinectes sapidus). | 1978 | 638269 | |
| a simple method for the computation of first neighbour frequencies of dnas from cd spectra. | a procedure for the computation of the first neighbour frequencies of dna's is presented. this procedure is based on the first neighbour approximation of gray and tinoco. we show that the knowledge of all the ten elementary cd signals attached to the ten double stranded first neighbour configurations is not necessary. one can obtain the ten frequencies of an unknown dna with the use of eight elementary cd signals corresponding to eight linearly independent polymer sequences. these signals can be ... | 1978 | 673843 |
| cytological observations on the blood and hemopoietic tissue in the crab, callinectes sapidus. i. the fine structure of hemocytes from intermolt animals. | a fine structural analysis of hemocytes recovered from the pericardial sinus of the blue crab (callinectes sapidus) demonstrates that hyaline cells, intermediate cells, and granulocytes are present in the hemolymph. a detailed consideration of their shared and differing cytological features suggests that the various blood cells represent different stages of cytogenesis along a common or single path of cellular differentiation. the potential functional complexity of the hemocytes is attested to b ... | 1978 | 204416 |
| recognition of viruses and xenogeneic proteins by the blue crab, callinectes sapidus. i. clearance and organ concentration. | 1977 | 205450 | |
| excitatory synapses of blue crab gastric mill muscles. | physiological and ultrastructural studies were made of neuromuscular synapses in stomach muscles, especially two gastric mill muscles of the blue crab innervated by neurons of the stomatogastric ganglion. these muscles depolarized and contracted with application of glutamate, but not acetylcholine, whereas the dorsal dilator muscles of the pyloric region depolarized and contracted in acetylcholine, but not in glutamate. large excitatory postsynaptic potentials (epsp's) of 5-20 mv were recorded i ... | 1977 | 837405 |
| fate of labeled n-alkanes in the blue crab and stripped mullet. | results of these studies demonstrate that the blue crab was readily able to take up and discharge labeled n-alkanes, but it was not able to metabolize them. in contrast, it appears that n-alkanes taken up via the digestive tract in mullet are readily metabolized, probably via microorganisms in the gut. more limited metabolism was observed in mullet when n-alkanes were taken up via the gills. | 1977 | 861400 |
| the subunit characterization of callinectes sapidus hemocyanin. | hemocyanin from the blue crab, callinectes sapidus, sediments at 25.7 s and has a native molecular weight of 940 000 +/- 20 000. under solution conditions of increased ph (approximately 10) or ionic strength, the native molecule dissociates to a 17 s species. reversal of this dissociation was unsuccessful. at ph 10 and with the removal of mg2+, the 17 s species reversibly dissociates to form a subunit species which sediments at 6 s. a comparison of the circular dichroic spectra of the 25.7 s and ... | 1977 | 14702 |
| cheliped laterality in callinectes sapidus (crustacea: portunidae). | 1976 | 953070 | |
| comparative studies of intra- and extramitochondrial calcium phosphates from the hepatopancreas of the blue crab (callinectes sapidus). | subcellular fractions of cytoplasmic mineral granules and mineral-loaded mitochondria were isolated from whole homogenates of hepatopancreas of the blue crab (callinectes sapidus). chemical, physical, and morphologic studies were carried out on both subcellular fractions, with and without prior removal of organic components by hydrazine extraction. in contrast to cytoplasmic granules, whole mitochondria contained appreciable amounts of mineral ions not associated with a solid mineral phase. quan ... | 1976 | 990969 |
| oxygen consumption of the blue crab, callinectes sapidus rathbun, from proecdysis to postecdysis. | 1976 | 3345 | |
| response of isolated axonal preparations to hyperosmoticity: evidence for a cellular volume regulation. | when submitted to a hyperosmotic stress, isolated axons of callinectes sapidus shrink. volume regulation is observed if the experiment is carried out by using serum from a crab adapted to sea water. 3' : 5'-amp and dibutyryl amp are also effective. a compound isolated from the hemolymph and having a molecular weight close to 10 000 mimics the effects of the serum and induces a partial regulation of the axonal volume. | 1976 | 65947 |
| gas-bubble disease in the blue crab, callinectes sapidus. | 1976 | 1254984 | |
| blue crab mortality: interaction of temperature and ddt residues. | serial observations of ddt-contaminated and uncontaminated salt marshes in the norther gulf of mexico were made in november and december, 1973. blue crab (callinectes sapidus) mortalities observed in the ddt-contaminated marsh during this period were correlated with reduced daily temperature minima. gas chromatographic analysis of hepatopancreas and swimmeret muscle tissue of dead and dying crabs revealed total ddt residue concentrations as high as 39.0 ppm and 1.43 ppm, respectively. it is sugg ... | 1976 | 1267483 |
| a minchinia-like haplosporidan parasitizing blue crabs, callinectes sapidus. | 1976 | 822104 | |
| variations in serum constituents of the blue crab, callinectes sapidus: copper and zinc. | 1975 | 234043 | |
| temperature effects on axonal conduction in callinectes sapidus (rathbun) and carcinus maenas (l.). | 1975 | 236872 | |
| volume regulation and alanine transport. response of isolated axons of callinectes sapidus rathbun to hypo-osmotic conditions. | 1975 | 236878 | |
| free amino acid changes in normal and eyestalkless megalopa larvae of the blue crab, callinectes sapidus, during the course of the molt cycle. | 1975 | 236891 | |
| the function of hemocyanin in respiration of the blue crab callinectes sapidus. | blood po2 in the blue crab callinectes sapidus, a very active species of tropical origin, is lower at 22 degrees c than that of larger crabs in colder waters. these low oxygen levels permit its hemocyanin to be highly oxygenated at the gill, and to deliver almost half of its oxygen to the tissues in resting animals. sustained muscular activity results in conspicuous decreases in blood po2, ph and hemocyanin oxygenation. although the venous reserve is fully utilized, hemocyanin oxygenation at the ... | 1975 | 240904 |
| the influence of environmental salinity on hemocyanin function in the blue crab, callinectes sapidus. | the effects of inorganic ions and of the hydrogen ion on oxygen-binding properties of most respiratory pigments are opposite. the addition of salt to the medium increases oxygen affinities, and the addition of h+ decreases oxygen affinities of crustacean hemocyanins. these oxygenation properties, as observed in vitro, suggest that the oxygen-transport system must adapt to ionic changes in the blood. in fact, decreases in the salt concentration of the blood of estaurine blue crabs are accompanied ... | 1975 | 240905 |
| ionic and osmotic regulation and metabolic response to salinity of juvenile callinectes sapidus rathbun. | 1975 | 241556 | |
| respiration rates and atp concentrations in the excised gills of the blue crab as a function of salinity. | 1975 | 1194 | |
| atomic structure of intracellular amorphous calcium phosphate deposits. | the radial distribution function calculated from x-ray diffraction of mineralized cytoplasmic structures isolated from the hepatopancreas of the blue crab (callinectes sapidus) is very similar to that previously found for synthetic amorphous calcium phosphate. both types of mineral apparently have only short-range atomic order, represented as a neutral ion cluster of about 10 a in longest dimension, whose probable composition is expressed by the formula ca9(po4)6. the minor differences observed ... | 1975 | 1056015 |
| bacterial flora of the hemolymph of the blue crab, callinectes sapidus: most probable numbers. | the hemolymph of 290 freshly collected blue crabs from chincoteague bay, va., was sampled over a 15-month period from august 1968 through november 1969 and most probable numbers of bacteria were determined by tube dilution. the hemolymph of 18% of all crabs sampled was found to be sterile, with 16% sterility in summer and 23% in winter samples. despite individual variations, male crabs as a group had a higher bacterial hemolymph burden than females, and among both sexes summer counts were higher ... | 1975 | 1090257 |
| bacterial flora of the hemolymph of the blue crab, callinectes sapidus: numerical taxonomy. | bacteria isolated from the hemolymph of normal blue crabs were found to be predominantly vibrio sp., pseudomonas sp., acinetobacter sp., bacillus sp., flavobacterium sp., and coliforms. vibrio parahaemolyticus, a cosmopolitan facultative pathogen widely implicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood, was present in crabs collected between the months of may to november and was identified in up to 21% of the hemolymphs sampled. | 1975 | 1090258 |
| a disease of the blue crab, callinectes sapidus, of possible viral etiology. | 1975 | 1151102 | |
| tonic immobility in the blue crab (callinectes sapidus, rathbun) its relation to threat of predation. | the tonic immobility. response in the blue crab (callinectes sapidus, rathbun) was investigated in a series of seven experiments. although reported to be a powerful variable in other species, preinduction electric shock produced inconsistent increases in the duration of tonic immobility with the blue crab; manipulations that were more directly relevant to the fear of predation had considerably greater effects than shock. physical damage to the chelipeds, mirror image stimulation, and immobilizat ... | 1975 | 1171124 |