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effect of a terminated cover crop and aldicarb on cotton yield and meloidogyne incognita population density.terminated small grain cover crops are valuable in light textured soils to reduce wind and rain erosion and for protection of young cotton seedlings. a three-year study was conducted to determine the impact of terminated small grain winter cover crops, which are hosts for meloidogyne incognita, on cotton yield, root galling and nematode midseason population density. the small plot test consisted of the cover treatment as the main plots (winter fallow, oats, rye and wheat) and rate of aldicarb ap ...200819259531
nutsedge counts predict meloidogyne incognita juvenile counts in an integrated management system.the southern root-knot nematode (meloidogyne incognita), yellow nutsedge (cyperus esculentus) and purple nutsedge (cyperus rotundus) are important pests in crops grown in the southern us. management of the individual pests rather than the pest complex is often unsuccessful due to mutually beneficial pest interactions. in an integrated pest management scheme using alfalfa to suppress nutsedges and m. incognita, we evaluated quadratic polynomial regression models for prediction of the number of m. ...200819259526
exposure time to lethal temperatures for meloidogyne incognita suppression and its implication for soil solarization.meloidogyne incognita eggs or j2 were incubated in test tubes containing sand:peat mix and immersed in a water bath heated to 38, 39, 40, 41, 42, 43, 44 and 45 degrees c for a series of time intervals. controls were maintained at 22 degrees c. nematodes surviving or hatching were collected from baermann trays after three weeks of incubation. regression analyses between percent survival or egg hatch and hours of heat treatment were performed for each temperature. complete suppression of egg hatch ...200819259512
dose-response effects of clove oil from syzygium aromaticum on the root-knot nematode meloidogyne incognita.clove oil, derived from the plant syzygium aromaticum (l.) merr. & perry, is active against various organisms, and was prepared in a soy lecithin/detergent formulation to determine concentrations active against the root-knot nematode meloidogyne incognita (kofoid and white) chitwood.200818080287
maize 9-lipoxygenase zmlox3 controls development, root-specific expression of defense genes, and resistance to root-knot nematodes.root-knot nematodes (rkn) are severe pests of maize. although lipoxygenase (lox) pathways and their oxylipin products have been implicated in plant-nematode interactions, prior to this report there was no conclusive genetic evidence for the function of any plant lox gene in such interactions. we showed that expression of a maize 9-lox gene, zmlox3, increased steadily and peaked at 7 days after inoculation with meloidogyne incognita rkn. mu-insertional lox3-4 mutants displayed increased attractiv ...200818052887
a transgressive segregation factor (rkn2) in gossypium barbadense for nematode resistance clusters with gene rkn1 in g. hirsutum.host plant resistance is an important strategy for managing root-knot nematode (meloidogyne incognita) in cotton (gossypium l.). here we report evidence for enhanced resistance in interspecific crosses resulting from transgressive segregation of clustered gene loci. recently, a major gene, rkn1, on chromosome 11 for resistance to m. incognita in cv. acala nemx was identified using an intraspecific g. hirsutum cross with susceptible cv. acala sj-2. using interspecific crosses of acala nemxxsuscep ...200817940800
low amounts of herbivory by root-knot nematodes affect microbial community dynamics and carbon allocation in the rhizosphere.increased carbon translocation to the rhizosphere via 'leakage' induced by low amounts of plant parasitic nematodes can foster microorganisms. the effects of the root-knot nematode meloidogyne incognita on microbial biomass (c(mic)) and community structure (phospholipid fatty acids) in the rhizosphere of barley were studied. inoculation densities of 2000, 4000, and 8000 nematodes were well below the threshold level for plant damage. a (13)co(2) pulse-labelling was performed to assess the distrib ...200717916076
transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism.root-knot nematodes of the genus meloidogyne are obligate biotrophic parasites able to infest > 2000 plant species. the nematode effectors responsible for disease development are involved in the adaptation of the parasite to its host environment and host response modulation. here, the differences between the transcriptomes of preparasitic exophytic second-stage juveniles (j2) and parasitic endophytic third-stage juveniles (j3) of meloidogyne incognita were investigated. genes up-regulated at the ...200717692078
cloning of the gene lecanicillium psalliotae chitinase lpchi1 and identification of its potential role in the biocontrol of root-knot nematode meloidogyne incognita.the nematophagous fungus lecanicillium psalliotae (syn. verticillium psalliotae) is a well-known biocontrol agent. in this study, a chitinase gene lpchi1 was isolated for the first time from l. psalliotae using degenerate primers and dna-walking technique. the cloned gene lpchi1 encoding 423 amino acid residues shares a high degree of homology with other pathogenicity-related chitinases from entomopathogenic and mycoparasitic fungi. the complementary dna sequence of the mature chitinase was ampl ...200717665191
nematicidal and larvicidal activities of the essential oils from aerial parts of pectis oligocephala and pectis apodocephala baker.the chemical composition of the essential oils from aerial parts of pectis apodocephala and pectis oligocephala were analyzed by gc-ms. the essential oils of these species were predominantly constituted by monoterpenes. geranial (42.9-44.5%), neral (32.2-34.2%) and alpha-pinene (10.7-11.4%) were the main constituents in the oil of p. apodocephala, while p-cymene (50.3-70.9%) and thymol (24.4-44.7%), were the prevalent compounds in the oil of p. oligocephala. the essential oils were tested agains ...200717625675
management of disease complex caused by root knot nematode and root wilt fungus on pigeonpea through soil organically enriched with vesicular arbuscular mycorrhiza, karanj (pongamia pinnata) oilseed cake and farmyard manure.this investigation was undertaken to compare the percentage response of colonization and development of va-mycorrhiza (glomus fasciculatum) on a number of pulse crops viz. cowpea, chickpea, soybean, pigeonpea and lentil under glasshouse conditions. among the above-mentioned crops, pigeonpea exhibited the best performance and was selected for further studies. in this host the development and colonization percentage of g. fasciculatum was investigated under two separate substrates i. e. soil amend ...200717978958
molecular cloning and analysis of a new venom allergen-like protein gene from the root-knot nematode meloidogyne incognita.a new venom allergen-like protein gene isolated from meloidogyne incognita (designated mi-vap-2) was cloned and analysed. the genomic clone of mi-vap-2 is 1917-bp long, contains three introns, which range in size from 39 to 797 bp, and four exons ranging in size from 37 to 361 bp. the cdna of mi-vap-2 contains an open reading frame encoding 294 amino acids, being the first 16 residues a putative secretion signal. southern blot analysis suggested that mi-vp-2 is probably a member of a small multi ...200717481609
ecotypes of the model legume lotus japonicus vary in their interaction phenotypes with the root-knot nematode meloidogyne incognita.knowledge of host factors affecting plant-nematode interactions is scarce. here, relevant interaction phenotypes between a nodulating model host, lotus japonicus, and the endoparasitic root-knot nematode meloidogyne incognita are assessed via a genetic screen.200717475628
coprinus comatus damages nematode cuticles mechanically with spiny balls and produces potent toxins to immobilize nematodes.we reported recently a unique fungal structure, called the spiny ball, on the vegetative hyphae of coprinus comatus (o. f. müll.:fr.) pers. although some observations regarding the role of this structure were presented, its function remained largely unknown. in this study, we showed that purified (isolated and washed) spiny balls could immobilize and kill the free-living nematode panagrellus redivivus goodey highly efficiently. scanning electron microscopy studies illustrated that the spiny stru ...200717449690
effect of broccoli (brassica oleracea) tissue, incorporated at different depths in a soil column, on meloidogyne incognita.brassicas have been used frequently for biofumigation, a pest-management strategy based on the release of biocidal volatiles during decomposition of soil-incorporated tissue. however, the role of such volatiles in control of plant-parasitic nematodes is unclear. the goal of this study was to determine the direct localized and indirect volatile effects of amending soil with broccoli tissue on root-knot nematode populations. meloidogyne incognita-infested soil in 50-cm-long tubes was amended with ...200719259479
variability in reproduction of four populations of meloidogyne incognita on six cultivars of cotton.a range of virulence levels was found in four populations of meloidogyne incognita collected from cotton fields of the punjab region of pakistan. the most virulent population was associated with development of larger gall size, larger giant cell formation and improved success of juveniles transitioning into reproducing adults. the most virulent nematode population, mi-78, emanated from cotton cultivar niab-78. this cotton cultivar also possessed the greatest level of resistance to the three othe ...200719259478
potential for site-specific management of meloidogyne incognita in cotton using soil textural zones.the effect of various edaphic factors on meloidogyne incognita population densities and cotton yield were evaluated from 2001 to 2003 in a commercial cotton field in southeastern arkansas. the 6.07-ha field was subdivided into 512 plots (30.5 m x 3.9 m), and each plot was sampled for m. incognita prior to fumigation (ppre), at planting (pi), at peak bloom (pm) and at harvest (pf) each year. soil texture (percent sand fraction) and the pre-plant soil fertility levels each year were determined fro ...200719259468
use of pepper crop residues for the control of root-knot nematodes.the biofumigant effect of pepper crop residues (pcr) for controlling meloidogyne incognita populations was evaluated. under laboratory conditions, 0, 5, 10 and 20 g pcr were applied to 500 g nematode infested soil, with four replicates per treatment. after 20 days at 25 degrees c, pcr reduced significantly m. incognita populations and root galling indices in susceptible tomato cv. marmande, and increased k, n and organic c in soil. in the field, biofumigation with pcr combined with fresh animal ...200717098424
meloidogyne incognita: molecular and biochemical characterisation of a cathepsin l cysteine proteinase and the effect on parasitism following rnai.rna interference has been used to investigate the function of a cathepsin l cysteine proteinase mi-cpl-1, in the plant-parasitic nematode meloidogyne incognita. a reduction in gene transcript was observed and the number of nematodes infecting plants was reduced by almost 60% as was the number of established females producing eggs at 21 days post-infection. the cysteine proteinase activity of m. incognita, reported by the substrate glupna, was inhibited by the cysteine proteinase inhibitor oc-ide ...200716996059
response of five lettuce cultivars to root-knot nematode, meloidogyne incognita.the root-knot nematode, meloidogyne incognito (kofoid et white) chitwood is an important pathogen of vegetables. five commercial cultivars of lettuce (lactuca sativa l.) were evaluated under greenhouse conditions for resistance to meloidogyne incognita, benguet population. plants were inoculated with 1000 eggs collected from 'apollo' tomato (lycopersicon esculentum) roots. the degree of galling and number of egg masses were assessed 4 and 8 weeks after inoculation. host plant response was classi ...200718399501
evaluation of soil biodesinfestation with crop and garden residues in the control of root-knot nematodes populations.fresh crop and garden residues were applied both under laboratory conditions and in commercial greenhouse in order to asses their effect on soil nematodes populations and soil fertility. in the laboratory experiments, dosages of 5 to 20 g of cabbage residues, chicken manure, cabbage residues+chicken manure, grass+chicken manure, as well as leaves and stems of orange tree, pine tree, oleander, olive tree, palm tree and boxwood were mixed with 500 g soil having root-knot nematodes (meloidogyne inc ...200718399508
responses of heterodera glycines and meloidogyne incognita to exogenously applied neuromodulators.biogenic amines regulate important behaviours in nematodes and are associated with pharyngeal activity in plant-parasitic nematodes. a robust behavioural assay based upon nematode body movements was developed to expand the study of these and other neuroregulators in plant-parasitic nematodes. dopamine, octopamine and serotonin each had significant but differing effects on the behaviour of soybean cyst nematode heterodera glycines and root-knot nematode meloidogyne incognita juveniles. body movem ...200718005465
solubilization of insoluble zinc compounds by gluconacetobacter diazotrophicus and the detrimental action of zinc ion (zn2+) and zinc chelates on root knot nematode meloidogyne incognita.to examine the zinc (zn) solubilization potential and nematicidal properties of gluconacetobacter diazotrophicus. methods and results: atomic absorption spectrophotometer, differential pulse polarography and gas chromatography coupled mass spectrometry were used to estimate the total zn and zn(2+) ions and identify the organic acids present in the culture supernatants. the effect of culture filtrate of zn-amended g. diazotrophicus pal5 on meloidogyne incognita in tomato was examined under gnotob ...200717309498
reniform in u.s. cotton: when, where, why, and some remedies.the reniform nematode, rotylenchulus reniformis, is an emerging problem in u.s. cotton. the impact of this nematode and the extent to which it has and will continue to spread across the u.s. cotton belt are controversial. long-term changes in cotton production and unique biological attributes of r. reniformis are key factors. expert opinion surveys indicate that r. reniformis has replaced the root-knot nematode (meloidogyne incognita) as the major nematode of cotton in mississippi, louisiana, an ...200717428179
resistance to root-knot nematode in tomato roots expressing a nematicidal bacillus thuringiensis crystal protein.our laboratory has demonstrated previously that bacillus thuringiensis (bt) crystal (cry) proteins present in the cry5 and cry6 subclades intoxicate free-living nematodes. in this study, we tested whether the expression of nematicidal cry6a in transgenic plants provided protection against plant-parasitic nematodes. as bacterial codon usage is incompatible with expression in plants, two different codon-modified cry6a genes were synthesized for expression in plants. one was designed by maintaining ...200717451491
cotton root protection from plant-parasitic nematodes by abamectin-treated seed.abamectin is nematicidal to meloidogyne incognita and rotylenchulus reniformis, but the duration and length of cotton taproot protection from nematode infection by abamectin-treated seed is unknown. based on the position of initial root-gall formation along the developing taproot from 21 to 35 d after planting, infection by m. incognita was reduced by abamectin seed treatment. penetration of developing taproots by both nematode species was suppressed at taproot length of 5 cm by abamectin-treate ...200719259471
the future of nematode management in cotton.the importance of plant-parasitic nematodes as yield-limiting pathogens of cotton has received increased recognition and attention in the united states in the recent past. this paper summarizes the remarks made during a symposium of the same title that was held in july 2007 at the joint meeting of the society of nematologists and the american phytopathological society in san diego, california. although several cultural practices, including crop rotation, can be effective in suppressing the popul ...200719259500
differential gene expression in arabidopsis following infection by plant-parasitic nematodes meloidogyne incognita and heterodera schachtii.summary whole genome microarrays were used to study plant gene expression in mature meloidogyne incognita-induced galls in arabidopsis. we found 959 genes to be significantly differentially expressed, and two-thirds of these were down-regulated. microarray results were confirmed by qrt-pcr. the temporal and spatial responses of four differentially expressed genes were analysed using gus reporter plants following infection with m. incognita and the cyst nematode heterodera schachtii. the ammonium ...200720507524
virulence of meloidogyne spp. and induced resistance in grape rootstocks.harmony grape rootstock displays resistance to several meloidogyne spp. but that resistance is not durable in commercial vineyard settings. a 2-year experiment in a microplot setting revealed host specificities of two virulent populations of meloidogyne arenaria and an avirulent population of meloidogyne incognita. in a subsequent split-root experiment, the avirulent nematode population was demonstrated to induce resistance to the virulent nematode population. to quantify the level of resistance ...200719259475
comprehensive transcriptome profiling in tomato reveals a role for glycosyltransferase in mi-mediated nematode resistance.root-knot nematode (rkn; meloidogyne spp.) is a major crop pathogen worldwide. effective resistance exists for a few plant species, including that conditioned by mi in tomato (solanum lycopersicum). we interrogated the root transcriptome of the resistant (mi+) and susceptible (mi-) cultivars 'motelle' and 'moneymaker,' respectively, during a time-course infection by the mi-susceptible rkn species meloidogyne incognita and the mi-resistant species meloidogyne hapla. in the absence of rkn infectio ...200717434994
plantago lanceolata and plantago rugelii extracts are toxic to meloidogyne incognita but not to certain microbes.extracts from the plants plantago lanceolata and p. rugelii were evaluated for toxicity to the root-knot nematode meloidogyne incognita, the beneficial microbes enterobacter cloacae, pseudomonas fluorescens and trichoderma virens, and the plant-pathogenic fungi fusarium oxysporum f. sp. gladioli, phytophthora capsici, pythium ultimum, and rhizoctonia solani. wild plants were collected, roots were excised from shoots, and the plant parts were dried and ground to a powder. one set of extracts (10% ...200619259537
expression and regulation of the arabidopsis thaliana cel1 endo 1,4 beta glucanase gene during compatible plant-nematode interactions.the root-knot nematode meloidogyne incognita is an obligate endoparasite of plant roots and stimulates elaborate modifications of selected root vascular cells to form giant cells for feeding. an arabidopsis thaliana endoglucanase (atcel1) promoter is activated in giant cells that were formed in atcel1::uida transgenic tobacco and arabidopsis plants. activity of the full-length atcel1 promoter was detected in root and shoot elongation zones and in the lateral root primordia. different 5' and inte ...200619259541
a fusarium wilt resistance gene in gossypium barbadense and its effect on root-knot nematode-wilt disease complex.abstract fusarium wilt, caused by the soilborne pathogen fusarium oxysporum f. sp. vasinfectum race 1, is a vascular disease in cotton (gossypium spp.), and is a component of a disease complex with root-knot nematodes (meloidogyne incognita). genetic analysis of two interspecific crosses (g. barbadense pima s-7 x g. hirsutum acala nemx and pima s-7 x acala sj-2) showed that one major gene (designated fov1) with allele dosage effect conferred resistance to f. oxysporum f. sp. vasinfectum race 1 i ...200618943146
sensitivity of meloidogyne incognita and rotylenchulus reniformis to abamectin.avermectins are macrocyclic lactones produced by streptomyces avermitilis. abamectin is a blend of b(1a) and b(1b) avermectins that is being used as a seed treatment to control plant-parasitic nematodes on cotton and some vegetable crops. no ld(50) values, data on nematode recovery following brief exposure, or effects of sublethal concentrations on infectivity of the plant-parasitic nematodes meloidogyne incognita or rotylenchulus reniformis are available. using an assay of nematode mobility, ld ...200619259453
use of trichoderma harzianum and trichoderma viride for the biological control of meloidogyne incognita on tomato.trichoderma harzianum and trichoderma viride were tested for their capacity to reduce the incidence of the root-knot nematode meloidogyne incognita on tomato. in vitro studies demonstrated that all tested isolates were effective in causing nematode mortality compared with the control. trichoderma slightly reduced nematode damage to tomato in vivo. treatment of the soil with the biocontrol agents before transplanting, improved control over treatment directly at transplanting. the trichoderma isol ...200617390844
development of controlled release formulations of carbofuran and evaluation of their efficacy against meloidogyne incognita.controlled release (cr) formulations of the insecti-nematicide carbofuran have been prepared using commercially available rosin, sodium carboxymethylcellulose and sodium carboxymethylcellulose with clay (bentonite, kaolinite, and fuller's earth). the kinetics of carbofuran release in soil from the different formulations were studied in comparison with that of the commercially available granules (3g). release from the commercial formulation was faster than with the new cr formulations. addition o ...200616787021
nematode infection and reproduction in transgenic and mutant arabidopsis and tobacco with an altered phenylpropanoid metabolism.transgenic and mutant arabidopsis and tobacco plants with altered phenylpropanoid metabolism were infected with the plant parasitic root knot nematode meloidogyne incognita to assess the effect of the transgene or mutation on nematode infection and reproduction. modifications in the lignin biosynthetic pathway which alter lignin composition in roots affected reproduction. in arabidopsis with increased levels of syringyl lignin, reproduction was lower than in wild-type plants, while in tobacco wi ...200616831845
gastrodia anti-fungal protein from the orchid gastrodia elata confers disease resistance to root pathogens in transgenic tobacco.diseases of agricultural crops are caused by pathogens from several higher-order phylogenetic lineages including fungi, straminipila, eubacteria, and metazoa. these pathogens are commonly managed with pesticides due to the lack of broad-spectrum host resistance. gastrodia anti-fungal protein (gafp; gastrodianin) may provide a level of broad-spectrum resistance due to its documented anti-fungal activity in vitro and structural similarity to insecticidal lectins. we transformed tobacco (nicotiana ...200616858580
use of crop residues for the control of meloidogyne incognita under laboratory conditions.this laboratory study evaluates the biofumigant effect of different organic materials with the aim of developing non-chemical alternatives for the management of meloidogyne incognita (kofoid & white) chitwood populations. sources of organic material from the production system were selected with the aim of reducing agricultural residue accumulation problems as well as decreasing the costs due to the use of chemical fertilizers and pesticides. the selected materials were residues from pepper, stra ...200616927410
integrated application of some compatible biocontrol agents along with mustard oil seed cake and furadan on meloidogyne incognita infecting tomato plants.experiments were carried out to study the effect of two fungal bioagents along with mustard oil cake and furadan against root knot nematode meloidogyne incognita infecting tomato under greenhouse condition. bioagents viz., paecilomyces lilacinus and trichoderma viride alone or in combination with mustard cake and furadan promoted plant growth, reduced number of galls/plant, egg masses/root system and eggs/egg mass. the fungal bioagents along with mustard cake and nematicide showed least nematode ...200617048300
colonization by the arbuscular mycorrhizal fungus glomus versiforme induces a defense response against the root-knot nematode meloidogyne incognita in the grapevine (vitis amurensis rupr.), which includes transcriptional activation of the class iii chitinase gene vch3.inoculation of the grapevine (vitis amurensis rupr.) with the arbuscular mycorrhizal (am) fungus glomus versiforme significantly increased resistance against the root-knot nematode (rkn) meloidogyne incognita. studies using relative quantitative reverse transcription-pcr (rqrt-pcr) analysis of grapevine root inoculation with the am fungus revealed an up-regulation of vch3 transcripts. this increase was greater than that observed following infection with rkn. however, inoculation of the mycorrhiz ...200616326755
a major gene mapped on chromosome xii is the main factor of a quantitatively inherited resistance to meloidogyne fallax in solanum sparsipilum.meloidogyne fallax is an emerging pest in europe and represents a threat for potato production. we report the mapping of genetic factors controlling a quantitative resistance against m. fallax identified in the solanum sparsipilum genotype 88s.329.15. when infected, this genotype develops a necrotic reaction at the feeding site of the juveniles and totally prevents their development to the female stage. a "f1" diploid progeny consisting of 128 individuals was obtained using the potato (s. tubero ...200616365760
isolation and characterization of another cdna encoding a chorismate mutase from the phytoparasitic nematode meloidogyne arenaria.a new cdna, named ma-cm-2, encoding a chorismate mutase (cm), has been isolated from meloidogyne arenaria. the full-length cdna, carrying the trans-spliced sl1 leader sequence, was 753-bp long with an open reading frame of 576 bp. the deduced protein ma-cm-2 including amino-terminal signal peptide shows significant similarity to cms of meloidogyne incognita, meloidogyne javanica, and also bacteria. secondary structure prediction of ma-cm-2 indicates the presence of the three conserved alpha-heli ...200616458297
characterization of a new beta-1,4-endoglucanase gene from the root-knot nematode meloidogyne incognita and evolutionary scheme for phytonematode family 5 glycosyl hydrolases.cellulases from plant parasitic nematodes are encoded by multiple gene families and are thought to originate from horizontal gene transfer. unraveling the evolution of these genes in the phylum will help understanding the evolution of plant parasitism in nematodes. here we describe a new gene, named mi-eng-2, that encodes a family 5 glycosyl hydrolase (ghf5) with a predicted signal peptide and devoid of linker domain and cellulose-binding domain. the beta-1,4-endoglucanase activity of the protei ...200616962258
qtl mapping for resistance to root-knot nematodes in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source.root-knot nematodes meloidogyne incognita (kofoid and white) can cause severe yield loss in cotton (gossypium hirsutum l.). the objectives of this study were to determine the inheritance and genomic location of genes conferring root-knot nematode resistance in m-120 rnr, a highly resistant g. hirsutum line with the auburn 623 rnr source of resistance. utilizing two interspecific f(2) populations developed from the same m-120 rnr by gossypium barbadense (cv. pima s-6) cross, genome-wide scanning ...200616960714
velvetbean (mucuna pruriens) extracts: impact on meloidogyne incognita survival and on lycopersicon esculentum and lactuca sativa germination and growth.velvetbean (mucuna spp.) is a summer annual that has been used as a cover crop to reduce erosion, fix nitrogen and suppress weeds and plant-parasitic nematodes. crude aqueous extracts (1:15 dry weight plant/volume water) were made from velvetbean plant parts, and various concentrations of the extracts were evaluated in vitro for toxicities to different stages of meloidogyne incognita (kofoid and white) chitwood and for suppression of hypocotyl and root growth and inhibition of germination of tom ...200616953489
the comparative cellular architecture of the female gonoduct among tylenchoidea (nematoda: tylenchina).the cellular architecture of the female gonoduct of 68 nematode populations representing 42 species belonging to tylenchidae, belonolaimidae, hoplolaimidae and meloinema is shown to have an overall similarity in cellular gonoduct structure. the oviduct consists of two rows of four cells; the spermatheca is comprised of 10 to 20 cells, and the uterus cells, except in the case of psilenchus, are arranged in four (tylenchidae) or three (belonolaimidae, hoplolaimidae and meloinema) regular rows. alt ...200619259542
amelioration of root-knot disease of lady's finger plants by potentized cina and santonin.lady's finger plants (hibiscus esculentus), grown in pots, were inoculated with the second-stage larvae (76+/-6) of root-knot nematodes meloidogyne incognita, starting 7 days later they were treated with cina 30c, santonin 30c or ethanol 30c by foliar spray for 10 consecutive days. the drugs in 90% ethanol were diluted with distilled water 1:1000 before application on plants. thirty days after the last treatment the plants were uprooted. cina 30c and santonin 30c reduced nematode infestation of ...200616815517
phenotypic expression of rkn1-mediated meloidogyne incognita resistance in gossypium hirsutum populations.the root-knot nematode meloidogyne incognita is a damaging pest of cotton (gossypium hirsutum) worldwide. a major gene (rkn1) conferring resistance to m. incognita was previously identified on linkage group a03 in g. hirsutum cv. acala nemx. to determine the patterns of segregation and phenotypic expression of rkn1, f(1), f(2), f(2:3), bc(1)f(1) and f(2:7) recombinant inbred lines (ril) from intraspecific crosses between acala nemx and a closely related susceptible cultivar acala sj-2 were inocu ...200619259455
efficacy of a novel nematicidal seed treatment against meloidogyne incognita on cotton.the efficacy of abamectin as a seed treatment for control of meloidogyne incognita on cotton was evaluated in greenhouse, microplot, and field trials in 2002 and 2003. treatments ranging from 0 to 100 g abamectin/100 kg seed were evaluated. in greenhouse tests 35 d after planting (dap), plants from seed treated with abamectin were taller than plants from nontreated seed, and root galling severity and nematode reproduction were lower where treated seed were used. the number of second stage juveni ...200619259454
a method for generating meloidogyne incognita males.a method for producing mass quantities of meloidogyne incognita males free from other developmental stages was developed. host plants were grown hydroponically to facilitate nematode harvest. pruning stress was shown to cause a higher percentage of juveniles to develop as males vs. a no-stress control. application of pruning stress in the first 48 hr post-inoculation was also shown to be more effective at driving male development than at later times.200619259447
a symbiont-independent endo-1,4-beta-xylanase from the plant-parasitic nematode meloidogyne incognita.substituted xylan polymers constitute a major part of the hemicellulose fraction of plant cell walls, especially in monocotyledons. endo-1,4-beta-xylanases (ec 3.2.1.8) are capable of hydrolyzing substituted xylan polymers into fragments of random size. many herbivorous animals have evolved intimate relationships with endosymbionts to exploit their enzyme complexes for the degradation of xylan. here, we report the first finding of a functional endo-1,4-beta-xylanase gene from an animal. the gene ...200616673939
effects of cina on root-knot disease of mulberry.root-knot disease of mulberry is caused by the nematode meloidogyne incognita. it has important economic implications for sericulture. the homeopathic medicines, cina mother tincture (mt) and potentised cina 200c, prepared from the flowering meristems of artemisia nilagirica (clarke) pamp, were applied by foliar spray on mulberry (morus alba l.) infected with m. incognita juveniles (j2). pretreatment (ending 6 days before inoculation) and post-treatment (starting 6 days after inoculation) schedu ...200616569626
dynamics of meloidogyne incognita virulence to resistance genes rk and rk in cowpea.the virulence index of three meloidogyne incognita field isolates to the resistance gene rk in cowpea was 0%, 75%, and 120%, with the index measured as reproduction on resistant plants as a percentage of the reproduction on susceptible plants. continuous culture of the 75% virulent isolate on susceptible tomato for more than 5 years (about 25 generations) resulted in virulence decline to about 4%. the rate of the decline in virulence was described by exponential decay, indicating the progressive ...200619259435
reproduction of meloidogyne incognita on winter cover crops used in cotton production.substantial reproduction of meloidogyne incognita on winter cover crops may lead to damaging populations in a subsequent cotton (gossypium hirsutum) crop. the amount of population increase during the winter depends on soil temperature and the host status of the cover crop. our objectives were to quantify m. incognita race 3 reproduction on rye (secale cereale) and several leguminous cover crops and to determine if these cover crops increase population densities of m. incognita and subsequent dam ...200619259434
root-knot nematode management in double-cropped plasticulture vegetables.combination treatments of chisel-injected fumigants (methyl bromide, 1,3-d, metam sodium, and chloropicrin) on a first crop, followed by drip-applied fumigants (metam sodium and 1,3-d +/- chloropicrin) on a second crop, with and without oxamyl drip applications were evaluated for control of meloidogyne incognita in three different tests (2002 to 2004) in tifton, ga. first crops were eggplant or tomato, and second crops were cantaloupe, squash, or jalapeno pepper. double-cropped vegetables suffer ...200619259431
screening of field pea (pisum sativum) selections for their reactions to root-knot nematode (meloidogyne incognita).pot studies were carried out to evaluate the reactions of 23 selections of field pea (pisum sativum) against meloidogyne incognita (2000 freshly hatched juveniles (j2)/pot). experiment was conducted under greenhouse conditions ((24.7+/-3) degrees c and (62+/-7)% rh) and terminated 45 d after inoculation. the roots of all the selections were assessed to determine root-knot indices (rki) on a 0-4 scale. out of 23 selections hfp-990713, pant p-25, and hfp-0129 were resistant; pant p-2005, ndp-2 and ...200616502508
atcat6, a sink-tissue-localized transporter for essential amino acids in arabidopsis.amino acids represent the major form of reduced nitrogen that is transported in plants. amino acid transporters in plants often show tissue-specific expression patterns and are used by plants to transport these metabolites from source to sink during development and under changing environmental conditions. we identified one amino acid transporter, atcat6, which is expressed in sink tissues such as lateral root primordia, flowers and seeds. additionally atcat6 was induced during infestation of roo ...200617052324
synthesis, characterization, and biotoxicity of n n donor sulphonamide imine silicon(iv) complexes.the organosilicon derivatives of 2-[1-(2-furayl)ethyledene]sulphathiazole with organosilicon chlorides have been synthesised and characterized on the basis of analytical, conductance, and spectroscopic techniques. probable trigonal bipyramidal and octahedral structures for the resulting derivatives have been proposed on the basis of electronic, ir, (1)h, (13)c nmr, and (29)si nmr spectral studies. in the search for better fungicides, bactericides, nematicides, and insecticides studies were condu ...200617496998
effect of different initial population densities of meloidogyne incognita on the growth of cicer arietinum.studies were undertaken to determine the effect of different initial population densities (pi = 50, 100, 250, 500, 1000, 2500, 5000, 10,000 and 15,000 j2/pot) of meloidogyn incognita on chikpea cv. bandal local in a pot experiment. plant growth, flowering and pod bearing were influenced by all the initial population densities. increase in pi not only resulted in proportional decrease in plant lenght, fresh weight, dry weight and pod bearing but also delayed the flowering. the highest reduction w ...200617390808
evaluation of a nematode bio-product dbx-20% against root-knot nematode meloidogyne incognita affecting grapevine under field conditions.a field trial was conducted in el-shourouk farm, el-beheira governorate, western nile valley, egypt to determine the effectiveness of the commercial bio-product dbx 1003 20% containing the nematode-trapping fungus dactylaria brochopaga against root-knot nematode meloidogyne incognita infesting grapevine variety superior. its effects on plant growth criteria and yield production were also investigated. the fungus was introduced to soil by either of two ways. first: soil was drenched with spore su ...200617390807
soil amendment with dried weed leaves as non-chemical approach for the management of meloidogyne incognita infecting tomato.in pot trial, dried ground weed leaves of cynodon dactylon, datura stramonium, eichhomia crassipes, emex spinosus, ricinus communis and sisymbrium irio were mixed with soil at the rate of 1, 3, 5 and 10 g/kg soil and compared their nematicidal potential with carbofuran as a standard against the root-knot nematode, m. incognita infecting tomato. in addition, their effects on growth rate of tomato plants were also investigated. the results showed that m. incognita populations in the soil and root ...200617612348
[interaction between structure of plant steroids and their effect on phytonematodes].the effects of certain plant steroids of the groups of furostanol glycosides, glycoalkaloids, and alpha-ecdysone on growth and development of phytoparasitic nematodes were studied. it was shown using an experimental system including tomato, lycopersicon esculentum mill., and root-knot nematode, meloidogyne incognita kofoid et white, that steroid molecule had significant nematicidic activity if it contained a carbohydrate moiety and an additional heterocycle in the steroid core. the maximum nemat ...200615029710
identification and mapping of microsatellite markers linked to a root-knot nematode resistance gene (rkn1) in acala nemx cotton (gossypium hirsutum l.).host-plant resistance is the most economic and effective strategy for root-knot nematode (rkn) meloidogyne incognita control in cotton (gossypium hirsutum l.). molecular markers linked to resistance are important for incorporating resistance genes into elite cultivars. to screen for microsatellite markers (ssr) closely linked to rkn resistance in g. hirsutum cv. acala nemx, f1, f2, bc1f1, and f2:7 recombinant inbred lines (rils) from intraspecific crosses and an f2 from an interspecific cross wi ...200616362274
a method for field infestation with meloidogyne incognita.a field inoculation method was developed to produce meloidogyne spp. infestation sites with minimal quantities of nematode inoculum and with a reduced labor requirement compared to previous techniques. in a preseason-methyl bromidefumigated site, nematode egg suspensions were delivered at concentrations of 0 or 10(x) eggs/m of row where x = 2.12, 2.82, 3.52, or 4.22 through a drip line attached to the seed firmer of a commercial 2-row planter into the open seed furrow while planting cowpea. thes ...200519262898
fitness of virulent meloidogyne incognita isolates on susceptible and resistant cowpea.a study of life-history traits was made to determine factors associated with the fitness of meloidogyne incognita isolates virulent to resistance gene rk in cowpea. egg hatch, root penetration, egg mass production, and fecundity (eggs per egg mass) of avirulent and virulent phenotypes were compared among m. incognita isolates, isofemale lines, and single descent lines over multiple generations on resistant and susceptible cowpea. variation (p </= 0.05) in both hatch and root penetration rates wa ...200519262891
isofemale line analysis of meloidogyne incognita virulence to cowpea resistance gene rk.isofemale lines (ifl) from single egg masses were studied for genetic variation in meloidogyne incognita isolates avirulent and virulent to the resistance gene rk in cowpea (vigna unguiculata). in parental isolates cultured on susceptible and resistant cowpea, the virulent isolate contained 100% and the avirulent isolate 7% virulent lineages. virulence was selected from the avirulent isolate within eight generations on resistant cowpea (lineage selection). in addition, virulence was selected fro ...200519262890
rna interference of dual oxidase in the plant nematode meloidogyne incognita.rna interference (rnai) is a powerful tool for the analysis of gene function in model organisms such as the nematode caenorhabditis elegans. recent demonstrations of rnai in plant parasitic nematodes provide a stimulus to explore the potential of using rnai to investigate disruption of gene function in meloidogyne incognita, one of the most important nematode pests of global agriculture. we have used rnai to examine the importance of dual oxidases (peroxidase and nadph oxidase), a class of enzym ...200516255249
use of cucumis metuliferus as a rootstock for melon to manage meloidogyne incognita.root-knot nematode-susceptible melons (cantaloupe) were grown in pots with varying levels of meloidogyne incognita and were compared to susceptible melons that were grafted onto cucumis metuliferus or cucurbita moschata rootstocks. in addition, the effect of using melons as transplants in nematode-infested soil was compared to direct seeding of melons in nematode-infested soil. there were no differences in shoot or root weight, or severity of root galling between transplanted and direct-seeded n ...200519262873
application of rna interference to root-knot nematode genes encoding esophageal gland proteins.plant parasitic nematodes have been, so far, refractory to transformation or mutagenesis. the functional analysis of nematode genes relies on the development of reverse genetic tools adapted to these obligate parasites. here, we describe the application of rna interference (rnai) to the root-knot nematode meloidogyne incognita for the knock-down of two genes expressed in the subventral esophageal glands of the nematode and potentially involved in parasitism, the calreticulin (mi-crt) and the pol ...200516042006
nematicidal natural products from the aerial parts of lantana camara linn.lantanilic acid, camaric acid and oleanolic acid possessing nematicidal activity were isolated from the methanolic extract of the aerial parts of lantana camara linn. through bio-assay guided fractionation. these compounds exhibited 98%, 95% and 70% mortality respectively against root-knot nematode meloidogyne incognita at 0.5% concentration. conventional nematicide furadan showed 100% mortality at this concentration.200516010828
effects of dl-methionine on hatching and activity of meloidogyne incognita eggs and juveniles.several concentrations of dl-methionine were tested in the laboratory for their effects on meloidogyne incognita (kofoid & white) chitwood egg hatching and juvenile activity in aqueous suspensions and infested soil. after 7 days in methionine solutions, the proportions of hatched eggs were reduced by 23.3% at 0.25 mg litre(-1) methionine and by 76.4% at 25 g litre(-1) when compared with controls in tap water. an effect of methionine solutions on juvenile activity was also apparent after 24 h in ...200515751006
evaluation of several approaches to manage meloidogyne incognita and cotton seedling disease complexes in the high plains of texas.field experiments were conducted for control of the southern root-knot nematode (meloidogyne incognita) and cotton seedling disease fungi (primarily thielaviopsis basicola) in one naturally infested field during 1999 and 2000 and in three additional fields in 2000. treatments included: seed-applied fungicides (triadimenol + mefenoxam + thiram and carboxin + pcnb + mefenoxam), cultivars (paymaster [pm] 2326 rr and pm 2200 rr), and a nematicide (aldicarb at 0.83 kg a.i/ha). plant stands were highe ...200519262844
resistance gene analogue markers are mapped to homeologous chromosomes in cultivated tetraploid cotton.degenerate primers designed from conserved motifs of known plant resistance gene products were used to amplify genomic dna sequences from the root-knot nematode (meloidogyne incognita) resistance genetic source, upland cotton (gossypium hirsutum) cultivar auburn 634 rnr. a total of 165 clones were isolated, and sequence analysis revealed 57 of the clones to be novel nucleotide sequences, many containing the resistance (r)-protein nucleotide-binding site motif. a cluster analysis was performed wi ...200515726317
an sxp/ral-2 protein produced by the subventral pharyngeal glands in the plant parasitic root-knot nematode meloidogyne incognita.meloidogyne incognita is a major parasite of numerous plant families, including many crop species. upon infection of the plant root, it induces several multinucleate giant cells by the injection of pharyngeal gland secretions into the root cells. in order to obtain a better understanding of the nematode-plant interaction, characterization of the pharyngeal gland secretions is a necessity. by differential display, a nematode gene was identified that encodes a new member of the sxp/ral-2 protein f ...200515565464
isolation and characterization of a cdna encoding a serine proteinase from the root-knot nematode meloidogyne incognita.this report describes the first serine proteinase gene isolated from the sedentary nematode meloidogyne incognita. using degenerate primers, a 1372bp cdna encoding a chymotrypsin-like serine proteinase (mi-ser1) was amplified from total rna of adult females by rt-pcr and 5' and 3' rapid amplification of cdna ends. the deduced amino acid sequence of mi-ser1 encoded a putative signal peptide and a prodomain of 22 and 33 amino acids, respectively, and a mature proteinase of 341 amino acids with a p ...200515888294
developmental expression and molecular analysis of two meloidogyne incognita pectate lyase genes.proteinaceous secretions from the oesophageal glands of plant-parasitic nematodes have crucial roles in nematode parasitism of plants. two cdnas (designated mi-pel-1 and mi-pel-2) encoding pectate lyases were isolated from the root-knot nematode, meloidogyne incognita, oesophageal gland-cell subtractive cdna libraries, and the corresponding genomic dnas were subsequently cloned. southern blot analyses revealed that homologues to these pectate lyase genes were broadly distributed in meloidogyne s ...200515862581
in planta secretion of a calreticulin by migratory and sedentary stages of root-knot nematode.esophageal secretions from endoparasitic sedentary nematodes are thought to play key roles throughout plant parasitism, in particular during the invasion of the root tissue and the initiation and maintenance of the nematode feeding site (nfs) essential for nematode development. the secretion in planta of esophageal cell-wall-degrading enzymes by migratory juveniles has been shown, suggesting a role for these enzymes in the invasion phase. nevertheless, the secretion of an esophageal gland protei ...200516478047
[effects of meloidogyne incognita initial inoculation density on ginger growth].the study with potted ginger showed that meloidogyne incognita infection could reduce the plant height, stem diameter, quantity of shoots, and fresh weight of stem, leaf and root, and finally, reduce ginger yield. with the enhancement of m. incognita initial inoculation density, the growth of ginger was damaged more severely, e. g., under inoculating 50, 100 and 200 m. incognita eggs per 100 g soil, ginger yield decreased by 27.91%, 37.73% and 42.14%, respectively. the reproduction rate of m. in ...200516471354
activity of hydroxamic acids from secale cereale against the plant-parasitic nematodes meloidogyne incognita and xiphinema americanum.abstract cyclic hydroxamic acids are secondary metabolites found in the family poaceae and have been implicated in the allelopathy of rye (secale cereale). the toxicity of these compounds against plant-parasitic nematodes is unknown. diboa (2,4-dihydroxy-(2h)-1,4-benzoxazin-3(4h)-one), dimboa (2,4-hydroxy-7-methoxy-(2h)-1,4-benzoxazin-3(4h)-one), and their degradation products boa (benzoxazolin-2(3h)-one) and mboa (6-methoxy-benzoxazolin-2(3h)-one) were screened in vitro against meloidogyne inco ...200518943462
studies on the efficacy of the antihelminthic antibiotic (kt-199) in the control of root-knot nematode meloidogyne incognita (kofoid and white) chitwood on tomato. 20056746308
biological control potential of the obligate parasite pasteuria penetransagainst the root-knot nematode, meloidogyne incognita infestation in brinjal.the efficacy of the obligate bacterial parasite, pasteuria penetrans against the rootknot nematode, meloidogyne incognita infestation was assessed in brinjal. the seedling pans with sterilized soil were inoculated with nematodes and root powder of p. penetrans were applied at different dosages viz., 0 x 10(6), 0.5 x 10(6) spores and 1 x 10(6) spores/pan. seeds of brinjal cv co2 were sown in the pans and seedlings were allowed to grow. the seedlings were transplanted to microplots containing ster ...200516628937
integrated management of root-knot nematode, meloidogyne incognita infestation in tomato and grapevine.an integrated approach with the obligate bacterial parasite, pasteuria penetrans and nematicides was assessed for the management of the root-knot nematode, meloidogyne incognita infestation in tomato and grapevine. seedlings of tomato cv. co3 were transplanted into pots filled with sterilized soil and inoculated with nematodes (5000 juveniles/pot). the root powder of p. penetrans at 10 mg/pot was applied alone and in combination with carbofuran at 6 mg/pot. application of p. penetrans along with ...200516628938
morphological variability and molecular phylogeny of the nematophagous fungus monacrosporium drechsleri.an isolate of the nematode-trapping fungus monacrosporium drechsleri was collected from cultures of the root-knot nematode meloidogyne arenaria that had been maintained on tomato roots in greenhouse pots in beltsville, maryland. the plant-parasitic nematodes heterodera glycines, meloidogyne incognita and pratylenchus zeae and the free-living nematodes caenorhabditis elegans and panagrellus redivivus were placed on colonies of m. drechsleri grown in petri dishes to study ability of the isolate to ...200516396348
impact of the nematophagous fungus pochonia chlamydosporia on nematode and microbial populations.the microbial and nematode populations associated with two plants (tomato and cabbage) inoculated with the nematophagous fungus, pochonia chlamydosporia var. chlamydosporia or root knot nematode (meloidogyne incognita), or both, were compared with those in unplanted controls. the dominant factor affecting culturable microbial populations was found to be the presence or absence of tomato plants. generally microbial colony counts were lowest in unplanted soil, small increases were associated with ...200516363363
phenylacetic acid-producing rhizoctonia solani represses the biosynthesis of nematicidal compounds in vitro and influences biocontrol of meloidogyne incognita in tomato by pseudomonas fluorescens strain cha0 and its gm derivatives.the aim of the present investigation was to determine the influence of rhizoctonia solani and its pathogenicity factor on the production of nematicidal agent(s) by pseudomonas fluorescens strain cha0 and its gm derivatives in vitro and nematode biocontrol potential by bacterial inoculants in tomato.200515610416
management of meloidogyne incognita on tomato by root-dip treatment in culture filtrate of the blue-green alga, microcoleus vaginatus.the nematicidal potential of culture filtrates of the blue-green alga, microcoleus vaginatus (cyanobacterium) was tested against meloidogyne incognita on tomato in pots under greenhouse conditions. prior to the transplantation of tomato seedling, roots were dipped in different concentrations (0.2%, 0.5%, 1%, 2%, 10%, 50% and 100%) of culture filtrate of m. vaginatus for 30 min. root-dip treatment reduced the root galling and final population of m. incognita and increased vegetative growth of pla ...200515792580
ex vitro composite plants: an inexpensive, rapid method for root biology.plant transformation technology is frequently the rate-limiting step in gene function analysis in non-model plants. an important tool for root biologists is the agrobacterium rhizogenes-derived composite plant, which has made possible genetic analyses in a wide variety of transformation recalcitrant dicotyledonous plants. the novel, rapid and inexpensive ex vitro method for producing composite plants described in this report represents a significant advance over existing composite plant inductio ...200516045479
studies on the management of root-knot nematode, meloidogyne incognita-wilt fungus, fusarium oxysporum disease complex of green gram, vigna radiata cv ml-1108.studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (azadirachta indica) seed powder at 50 mg/kg soil, green mould (trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, meloidogyne incognita-wilt fungus, fusarium oxysporum disease complex on green gram, vigna radiata cv ml-1108. all the treatments significantly improved ...200516052706
effects of fly ash, pseudomonas striata and rhizobium on the reproduction of nematode meloidogyne incognita and on the growth and transpiration of pea.glasshouse experiments were conducted twice to assess the ash amendments (0, 20, and 40% with soil), a phosphate solubilizing microorganism pseudomonas striata and a root-nodule bacterium rhizobium sp on the reproduction of root-knot nematode meloidogyne incognita and on the growth and transpiration of pea. amendments of fly ash with soil had no effect on transpiration. however, m. incognita reduced the rate of transpiration from 1st week onward after inoculation while inoculation of rhizobium s ...200516114471
extracellular protease of pseudomonas fluorescens cha0, a biocontrol factor with activity against the root-knot nematode meloidogyne incognita.in pseudomonas fluorescens cha0, mutation of the gaca-controlled apra gene (encoding the major extracellular protease) or the gaca regulatory gene resulted in reduced biocontrol activity against the root-knot nematode meloidogyne incognita during tomato and soybean infection. culture supernatants of strain cha0 inhibited egg hatching and induced mortality of m. incognita juveniles more strongly than did supernatants of apra and gaca mutants, suggesting that apra protease contributes to biocontro ...200516151170
root-knot nematode problem of some winter ornamental plants and its biomanagement.a microplot study under field conditions was carried out during 2 consecutive years to assess the effect of root-knot nematode infection (2,000 meloidogyne incognita eggs/kg soil) on three winter ornamental plants: hollyhock (althea rosea), petunia (petunia hybrida), and poppy (papaver rhoeas). effects of root-dip treatment with the biocontrol agents pochonia chlamydosporia, bacillus subtilis, and pseudomonas fluorescens and the nematicide fenamiphos were tested. the three ornamental species wer ...200519262861
reproduction of belonolaimus longicaudatus, meloidogyne javanica, paratrichodorus minor, and pratylenchus brachyurus on pearl millet (pennisetum glaucum).pearl millet (pennisetum glaucum) has potential as a grain crop for dryland crop production in the southeastern united states. whether or not pearl millet will be compatible in rotation with cotton (gossypium hirsutum), corn (zea mays), and peanut (arachis hypogaea) will depend, in part, on its host status for important plant-parasitic nematodes of these crops. the pearl millet hybrid 'tifgrain 102' is resistant to both meloidogyne incognita race 3 and m. arenaria race 1; however, its host statu ...200519262863
factors affecting the suppression of heterodera glycines by n-viro soil.previous laboratory research demonstrated that n-viro soil (nvs), an alkaline-stabilized municipal biosolid, suppressed plant-parasitic nematodes. this study continued to explore the use of nvs as a nematode management tool specifically addressing factors that could influence its use. n-viro soil from different locations, the components of nvs (de-watered biosolids and fly ash admixtures), and sterilized nvs were applied to sand microcosms to determine effects on nematode survival sand solution ...200519262864
variability of meloidogyne exigua on coffee in the zona da mata of minas gerais state, brazil.minas gerais is the major coffee-producing state of brazil, with 28% of its production coming from the region of zona da mata. four major species of root-knot nematode attacking coffee (meloidogyne incognita, m. paranaensis, m. coffeicola, and m. exigua) have been reported from brazil. to determine the variability in meloidogyne spp. occurring in that region, 57 populations from 20 localities were evaluated for morphological, enzymatic, and physiological characteristics. according to the perinea ...200519262880
effect of soils from six management systems on root-knot nematodes and plant growth in greenhouse assays.the effects of soil management systems on root-knot nematode (meloidogyne incognita) eggs and gall incidence on tomato (lycopersicon esculentum) and cucumber (cucumis sativus) following tomato were evaluated. soil was collected from a replicated field experiment in which six management systems were being assessed for vegetable production. soil management systems were conventional production, organic production, bahiagrass (paspalum notatum) pasture, bahiagrass: stylosanthes (stylosanthes guianen ...200519262892
effect of three plant residues and chicken manure used as biofumigants at three temperatures on meloidogyne incognita infestation of tomato in greenhouse experiments.plant residues of broccoli, melon, and tomato with or without addition of chicken manure were used as biofumigants in two pot experiments with meloidogyne incognita-infested soils. the efficacy of these biofumigants in controlling m. incognita infestation in susceptible tomato bio-assay plants was studied at soil temperatures of 20 masculine, 25 masculine, and 30 masculinec. none of the plant residues was effective at 20 masculinec, and broccoli was more effective than tomato or melon at 25 masc ...200519262896
two chorismate mutase genes from the root-knot nematode meloidogyne incognita.summary parasitism genes encoding secretory proteins expressed in the oesophageal glands of phytoparasitic nematodes play critical roles in nematode invasion of host plants, establishment of feeding sites and suppression of host defences. two chorismate mutase (cm) genes potentially having a role in one or more of these processes were identified from a meloidogyne incognita oesophageal gland-cell subtractive cdna library. these m. incognita enzymes (designated as mi-cm-1 and mi-cm-2) with amino- ...200520565635
production of auxin and related compounds by the plant parasitic nematodes heterodera schachtii and meloidogyne incognita.mass spectrometric analysis revealed the presence of auxin, mainly in conjugated form, in secretions of heterodera schachtii and meloidogyne incognita, with or without treatment with dmt or resorcinol. m. incognita showed the highest production rates, though treatment of m. incognita with resorcinol had a negative effect on auxin production. analysis of auxin precursor molecules in lysates of h. schachtii, m. incognita and caenorhabditis elegans suggested that auxin is most probably a degradatio ...200516363359
differential activation of abi3 and lea genes upon plant parasitic nematode infection.summary promoter activity of abi3 and of three lea genes was monitored in arabidopsis transgenics infected with heterodera schachtii and meloidogyne incognita. abi3::gus expression was induced (in four different promoter deletion constructs) during early infection stages with h. schachtii. similar gus expression patterns, though slightly later in time compared with abi3, were observed for one of the lea promoter constructs, whereas the other two were not induced by h. schachtii. expression was m ...200520565660
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