Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| prevalence of hiv infection and risky sexual practices among adult males with travel-associated entamoeba histolytica infection in taiwan, 2006-2013. | 2015 | 25593040 | |
| notes from the field: outbreak of diarrheal illness caused by shigella flexneri - american samoa, may-june 2014. | on may 9, 2014, a physician at hospital a in american samoa noticed an abnormally high number of children presenting to the emergency department with bloody diarrhea. based on preliminary testing of stool specimens, entamoeba histolytica infection was suspected as a possible cause. shigella was also suspected in a subset of samples. on may 22, the american samoa department of health requested assistance from cdc with the outbreak investigation. the goals of the investigation were to establish th ... | 2015 | 25590684 |
| copro prevalence and estimated risk of entamoeba histolytica in diarrheic patients at beni-suef, egypt. | amoebiasis diagnosis is usually based on microscopy that cannot differentiate pathogenic e. histolytica from morphologically identical non-pathogenic species. 194 fecal samples were collected from diarrheic &/or dysenteric patients and examined for entamoeba complex microscopically, e. histolytica/e. dispar coproantigen using ict and e. histolytica coproantigen using tech lab e. histolytica ii elisa test. entamoeba complex trophozoites/cysts, e. histolytica/e. dispar coproantigen and e. histolyt ... | 2015 | 25542044 |
| entamoeba histolytica racc selectively engages p21-activated kinase effectors. | rho family gtpases modulate actin cytoskeleton dynamics by signaling through multiple effectors, including the p21-activated kinases (paks). the intestinal parasite entamoeba histolytica expresses ∼20 rho family gtpases and seven isoforms of pak, two of which have been implicated in pathogenesis-related processes such as amoebic motility and invasion and host cell phagocytosis. here, we describe two previously unstudied pak isoforms, ehpak4 and ehpak5, as highly specific effectors of ehracc. a s ... | 2015 | 25529118 |
| serosurvey of entamoeba histolytica exposure among tepehuanos population in durango, mexico. | the seroepidemiology of entamoeba histolytica infection in tepehuanos population in mexico is largely unknown. this study aimed to study the seroprevalence and correlates of e. histolytica antibodies in tepehuanos in durango, mexico. through a cross-sectional study, we determined the frequency of e. histolytica igg antibodies in 156 tepehuanos people in durango, mexico using an enzyme-linked immunoassay. furthermore, we studied the association of e. histolytica seroprevalence with the socio-demo ... | 2015 | 26199578 |
| antiamoebic and antigiardial activity of clerodane diterpenes from mexican salvia species used for the treatment of diarrhea. | terpenoids from salvia species have been identified to possess biological properties as antiprotozoal agents. here, we evaluated the antiamoebic and antigiardial activities of 14 known clerodane and modified clerodane-type diterpenes isolated from five mexican salvia species against entamoeba histolytica and giardia lamblia, and analyzed the effects of the functionalities in decalin ring or in the whole clerodane framework to visualize the structural requirements necessary to produce an antiprot ... | 2015 | 26190323 |
| metronidazole hydrazone conjugates: design, synthesis, antiamoebic and molecular docking studies. | metronidazole hydrazone conjugates (2-13) were synthesized and screened in vitro for antiamoebic activity against hm1: imss strain of entamoeba histolytica. six compounds were found to be better inhibitors of e. histolytica than the reference drug metronidazole. these compounds showed greater than 50-60% viability against hela cervical cancer cell line after 72 h treatment. also, molecular docking study was undertaken on e. histolytica thioredoxin reductase (ehthrase) protein which showed signif ... | 2015 | 26174554 |
| molecular biology research to benefit patients with entamoeba histolytica infection. | the development of molecular microbiology has made it possible for us to deepen our understanding of the pathogenesis of amebiasis. research using the trophozoite form of entamoeba histolytica has clearly shown us the importance of the interface between the parasite and host cells in vitro. immuno-pathogenesis after excystation was similarly well advanced by the use of a novel murine model of amebic colitis. however, it is still challenging to apply these findings to clinical and epidemiological ... | 2015 | 26173474 |
| dimethylated h3k27 is a repressive epigenetic histone mark in the protist entamoeba histolytica and is significantly enriched in genes silenced via the rnai pathway. | rna interference (rnai) is a fundamental biological process that plays a crucial role in regulation of gene expression in many organisms. transcriptional gene silencing (tgs) is one of the important nuclear roles of rnai. our previous data show that entamoeba histolytica has a robust rnai pathway that links to tgs via argonaute 2-2 (ago2-2) associated 27-nucleotide small rnas with 5'-polyphosphate termini. here, we report the first repressive histone mark to be identified in e. histolytica, dime ... | 2015 | 26149683 |
| in entamoeba histolytica, a bspa family protein is required for chemotaxis toward tumour necrosis factor. | entamoeba histolytica cell migration is essential for the development of human amoebiasis (an infectious disease characterized by tissue invasion and destruction). the tissue inflammation associated with tumour necrosis factor (tnf) secretion by host cells is a well-documented feature of amoebiasis. tumour necrosis factor is a chemoattractant for e. histolytica, and the parasite may have a tnf receptor at its cell surface. | 2015 | 28357299 |
| entamoeba histolytica - tumor necrosis factor: a fatal attraction. | 2015 | 28357296 | |
| magnetic resonance imaging of pathogenic protozoan parasite entamoeba histolytica labeled with superparamagnetic iron oxide nanoparticles. | the aim of this study was to establish a noninvasive tracking of the pathogenic parasite entamoeba histolytica (eh) after superparamagnetic iron oxide (spio) labeling by magnetic resonance imaging (mri) on a single-cell level in vitro and in vivo in a mouse model for amebic liver abscess (ala). | 2015 | 26135016 |
| detection of entamoeba histolytica by recombinase polymerase amplification. | amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. traditional microscopy approaches are neither sensitive nor specific for entamoeba histolytica. antigen assays are more specific, but many cases are missed unless tested by molecular methods. although polymerase chain reaction (pcr) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource- ... | 2015 | 26123960 |
| [drainage of amoebic liver abscess by single incision laparoscopic surgery. report of a case]. | single incision laparoscopic surgery has increased recently due to successful results, achieved in several procedures. the aim of the present work is to present the first case in which single incision laparoscopy is used for the drainage of an amoebic liver abscess. | 2015 | 26118779 |
| molecular detection of the carriage rate of four intestinal protozoa with real-time polymerase chain reaction: possible overdiagnosis of entamoeba histolytica in nigeria. | diarrhea remains the second largest killer of children worldwide, and nigeria ranks number two on the list of global deaths attributable to diarrhea. meanwhile, prevalence studies on potentially diarrheagenic protozoa in asymptomatic carriers using molecular detection methods remain scarce in sub-saharan countries. to overcome sensitivity issues related to microscopic detection and identification of cysts in stool concentrates, real-time polymerase chain reaction (pcr) was used to analyze genomi ... | 2015 | 26101274 |
| insights into endosomal maturation of human holo-transferrin in the enteric parasite entamoeba histolytica: essential roles of rab7a and rab5 in biogenesis of giant early endocytic vacuoles. | the pathogenic amoeba entamoeba histolytica is one of the causative agents of health hazards in tropical countries. it causes amoebic dysentery, colitis and liver abscesses in human. iron is one of the essential nutritional resources for survival and chronic infection caused by the amoeba. the parasite has developed multiple ways to import, sequester and utilize iron from various iron-binding proteins from its host. in spite of its central role in pathogenesis, the mechanism of iron uptake by th ... | 2015 | 26096601 |
| protection against amoebic liver abscess in hamster by intramuscular immunization with an autographa californica baculovirus driving the expression of the gal-lectin lc3 fragment. | in a previous study, we demonstrated that oral immunization using autographa californica baculovirus driving the expression of the gal-lectin lc3 fragment (acnpv-lc3) of entamoeba histolytica conferred protection against ala development in hamsters. in this study, we determined the ability of acnpv-lc3 to protect against ala by the intramuscular route as well as the liver immune response associated with protection. results showed that 55% of hamsters im immunized with acnpv-lc3 showed sterile pr ... | 2015 | 26090442 |
| binding and endocytosis of bovine hololactoferrin by the parasite entamoeba histolytica. | entamoeba histolytica is a human parasite that requires iron (fe) for its metabolic function and virulence. bovine lactoferrin (b-lf) and its peptides can be found in the digestive tract after dairy products are ingested. the aim of this study was to compare virulent trophozoites recently isolated from hamster liver abscesses with nonvirulent trophozoites maintained for more than 30 years in cultures in vitro regarding their interaction with iron-charged b-lf (b-holo-lf). we performed growth kin ... | 2015 | 26090404 |
| cytotoxicity of protein-carbon nanotubes on j774 macrophages is a functionalization grade-dependent effect. | carbon nanotubes (cnts) are used as carriers in medicine due to their ability to be functionalized with chemical substances. however, cytotoxicity analysis is required prior to use for in vivo models. the aim of this study was to evaluate the cytotoxic effect of cnts functionalized with a 46 kda surface protein from entamoeba histolytica (p46-cnts) on j774a macrophages. with this purpose, cnts were synthesized by spray pyrolysis and purified (p-cnts) using sonication for 48 h. a 46 kda protein, ... | 2015 | 26075262 |
| chew on this: amoebic trogocytosis and host cell killing by entamoeba histolytica. | entamoeba histolytica was named 'histolytica' (from histo-, 'tissue'; lytic-, 'dissolving') for its ability to destroy host tissues. direct killing of host cells by the amoebae is likely to be the driving factor that underlies tissue destruction, but the mechanism was unclear. we recently showed that, after attaching to host cells, amoebae bite off and ingest distinct host cell fragments, and that this contributes to cell killing. we review this process, termed 'amoebic trogocytosis' (trogo-, 'n ... | 2015 | 26070402 |
| potential amoebicidal activity of hydrazone derivatives: synthesis, characterization, electrochemical behavior, theoretical study and evaluation of the biological activity. | four new hydrazones were synthesized by the condensation of the selected hydrazine and the appropriate nitrobenzaldehyde. a complete characterization was done employing 1h- and 13c-nmr, electrochemical techniques and theoretical studies. after the characterization and electrochemical analysis of each compound, amoebicidal activity was tested in vitro against the hm1:imss strain of entamoeba histolytica. the results showed the influence of the nitrobenzene group and the hydrazone linkage on the a ... | 2015 | 26035095 |
| heat shock protein 90 inhibitors repurposed against entamoeba histolytica. | hsp90 is an essential chaperone responsible for trafficking a vast array of client proteins, which are substrates that hsp90 regulates in eukaryotic cells under stress conditions. the atp-binding n-terminal domain of hsp90 (also known as a ghkl type atpase domain) can serve as a specific drug target, because sufficient structural diversity in the atp-binding pocket of hsp90 allows for ortholog selectivity of hsp90 inhibitors. the primary objective of this study is to identify inhibitors specific ... | 2015 | 26029171 |
| synthesis, characterization and antiamoebic activity of chalcones bearing n-substituted ethanamine tail. | a series of chalcones (4-21) possessing n-substituted ethanamine were synthesized by the aldol condensation reaction of 1-(4-(2-substituted ethoxy)phenyl)ethanones with different aldehydes preceded by the reaction of 2-chloro n-substituted ethanamine hydrochloride and 4-hydroxy acetophenone. the structure of all the synthesized compounds was elucidated by various spectral and x-ray diffraction studies. the compounds were screened against hm1: imss strain of entamoeba histolytica and cytotoxicity ... | 2015 | 26021707 |
| palladium(ii) complexes of os donor n-(di(butyl/phenyl)carbamothioyl)benzamide and their antiamoebic activity. | two promising palladium(ii) compounds of general formula, cis-[pd(l-o,s)2] [where hl-o,s = n-(di(butyl/phenyl)carbamothioyl)benzamide] as metal based antiamoebic drug candidates, have been synthesized. both complexes are characterized in the solid state by ft-ir spectroscopy, tga and single crystal x-ray study, as well as in solution by other spectroscopic techniques such as (1)h and (13)c nmr, and uv-visible. all these studies confirm the coordination of ligands through oxygen and sulphur atoms ... | 2015 | 26005916 |
| parasitism in children aged three years and under: relationship between infection and growth in rural coastal kenya. | parasitic infections, which are among the most common infections worldwide, disproportionately affect children; however, little is known about the impact of parasitic disease on growth in very early childhood. our objective was to document the prevalence of parasitic infections and examine their association with growth during the first three years of life among children in coastal kenya. | 2015 | 25996157 |
| a rare case of multilocus brain abscess due to entamoeba histolytica infection in a child. | 2015 | 25987125 | |
| entamoeba mitosomes play an important role in encystation by association with cholesteryl sulfate synthesis. | hydrogenosomes and mitosomes are mitochondrion-related organelles (mros) that have highly reduced and divergent functions in anaerobic/microaerophilic eukaryotes. entamoeba histolytica, a microaerophilic, parasitic amoebozoan species, which causes intestinal and extraintestinal amoebiasis in humans, possesses mitosomes, the existence and biological functions of which have been a longstanding enigma in the evolution of mitochondria. we previously demonstrated that sulfate activation, which is not ... | 2015 | 25986376 |
| amoeboma-lest we forget!!! | amoebiasis is an infection with the parasitic intestinal protozoan entamoeba histolytica (e. histolytica). most infections are usually asymptomatic, but the disease spectrum can range from dysentery to extraintestinal infections, including liver abscesses. the management of a 68-year-old male with amoeboma, a rare manifestation of intestinal amoebiasis, is described along with a review of literature. | 2015 | 25972693 |
| [coecum amoebiasis can be a rare manifestation of entamoeba histolytica infection]. | a 58-year-old danish male was admitted to hospital with acute abdominal pain. laparoscopy showed a normal appendix. however, a tumour in the ascending colon was found and hemicolectomy was performed. pathological examination showed deep ulcerations with undermined edges and massive inflammation and necrosis. periodic acid schiff (pas)-positive amoebae trophozoites were identified, and the patient was diagnosed with and treated for amoebiasis. the patient had not been abroad for 14 years but he w ... | 2015 | 25967092 |
| iron status of schoolchildren (6-15 years) and associated factors in rural nigeria. | schoolchildren are vulnerable to anaemia because of their higher iron need to meet the demands of puberty and adolescence. | 2015 | 25952679 |
| structure of an adp-ribosylation factor, arf1, from entamoeba histolytica bound to mg(2+)-gdp. | entamoeba histolytica is the etiological agent of amebiasis, a diarrheal disease which causes amoebic liver abscesses and amoebic colitis. approximately 50 million people are infected worldwide with e. histolytica. with only 10% of infected people developing symptomatic amebiasis, there are still an estimated 100,000 deaths each year. because of the emergence of resistant strains of the parasite, it is necessary to find a treatment which would be a proper response to this challenge. adp-ribosyla ... | 2015 | 25945714 |
| structures of a histidine triad family protein from entamoeba histolytica bound to sulfate, amp and gmp. | three structures of the histidine triad family protein from entamoeba histolytica, the causative agent of amoebic dysentery, were solved at high resolution within the seattle structural genomics center for infectious disease (ssgcid). the structures have sulfate (pdb entry 3oj7), amp (pdb entry 3omf) or gmp (pdb entry 3oxk) bound in the active site, with sulfate occupying the same space as the α-phosphate of the two nucleotides. the c(α) backbones of the three structures are nearly superimposabl ... | 2015 | 25945711 |
| characterization of ph-induced transitions of entamoeba histolytica d-phosphoglycerate dehydrogenase. | entamoeba histolytica d-phosphoglycerate dehydrogenase (ehpgdh) exists as a functionally active homodimer at ph 7. our earlier studies have shown that ionic interactions are essentially required for the oligomeric status and activity of the protein. present study focuses on ph associated structural modulations of ehpgdh. far-uv cd spectra showed loss in the secondary structure of the protein as a function of low ph, however, the protein was not completely unfolded even at ph 2. energy minimized ... | 2015 | 25944370 |
| amino acid residues leu135 and tyr236 are required for rna binding activity of cfim25 in entamoeba histolytica. | pre-mrna 3' end processing in the nucleus is essential for mrna stability, efficient nuclear transport, and translation in eukaryotic cells. in human, the cleavage/polyadenylation machinery contains the 25 kda subunit of the cleavage factor im (cfim25), which specifically recognizes two ugua elements and regulates the assembly of polyadenylation factors, poly(a) site selection and polyadenylation. in entamoeba histolytica, the protozoan parasite responsible for human amoebiasis, ehcfim25 has bee ... | 2015 | 25941172 |
| detection and molecular characterisation of giardia duodenalis, cryptosporidium spp. and entamoeba spp. among patients with gastrointestinal symptoms in gambo hospital, oromia region, southern ethiopia. | to assess the prevalence and genetic diversity of the enteric protozoa species g. duodenalis, cryptosporidium spp. and entamoeba histolytica in individuals with gastrointestinal symptoms compatible with infections by these pathogens seeking medical attention in a rural area in southern ethiopia. | 2015 | 25939247 |
| atg8 is involved in endosomal and phagosomal acidification in the parasitic protist entamoeba histolytica. | autophagy is one of two major bulk protein degradation systems and is conserved throughout eukaryotes. the protozoan entamoeba histolytica, which is a human intestinal parasite, possesses a restricted set of autophagy-related (atg) proteins compared with other eukaryotes and thus represents a suitable model organism for studying the minimal essential components and ancestral functions of autophagy. e. histolytica possesses two conjugation systems: atg8 and atg5/12, although a gene encoding atg12 ... | 2015 | 25923949 |
| [amoebic liver abscess in a patient initially diagnosed with pneumonia: case report and discussion of relevant literature]. | in one-third of the patients with amoebiasis, amoebic liver abscess (ala) may occur after the penetration of amoebic trophozoites through the intestinal wall. ala is seen mostly among men aged 20-45 years with a serious clinical outcome, with fever and abdominal pain on the right upper quadrant. most patients have no recent history of amoebic colitis; indeed, they have neither gastrointestinal complaints nor entamoeba histolytica (e. histolytica) cysts/trophozoites in their stools. therefore, ul ... | 2015 | 25917589 |
| identification of entamoeba histolytica by molecular method in surface water of rasht city, iran. | this study was conducted with the aim of determining surface water contamination with cysts of entamoeba histolytica using pcr in rasht city, northern iran. | 2015 | 25905058 |
| entamoeba histolytica: protein arginine transferase 1a methylates arginine residues and potentially modify the h4 histone. | in eukaryotes, histone arginine methylation associates with both active and repressed chromatin states depending on the residues involved and the status of methylation. even when the amino-terminus of entamoeba histolytica histones diverge from metazoan sequences, these regions contain arginine residues that are potential targets for methylation. however, histone arginine methylation as well as the activity of arginine methyltransferases (prmts) has not been studied in this parasite. the aim of ... | 2015 | 25889855 |
| entamoeba histolytica rhomboid protease 1 has a role in migration and motility as validated by two independent genetic approaches. | rhomboid proteins represent a recently discovered family of intramembrane proteases present in a broad range of organisms and with increasing links to human diseases. the enteric parasite entamoeba histolytica has evolved multiple mechanisms to adapt to the human host environment and establish infection. our recent studies identified ehrom1 as a functional e. histolytica rhomboid protease with roles in adhesion to and phagocytosis of host cells. since those studies were performed in a non-virule ... | 2015 | 25889553 |
| chemokine levels and parasite- and allergen-specific antibody responses in children and adults with severe or uncomplicated plasmodium falciparum malaria. | chemokine and antibody response profiles were investigated in children and adults with severe or uncomplicated plasmodium falciparum malaria; the aim was to reveal which profiles are associated with severe disease, as often seen in nonimmune children, or with mild and uncomplicated disease, as seen in semi-immune adults. blood samples were obtained from children under 5 years of age as well as adults with falciparum malaria. classification of malaria was performed according to parasite densities ... | 2015 | 25883801 |
| seroepidemiology of entamoeba histolytica infection in general population in rural durango, mexico. | the seroepidemiology of entamoeba histolytica in mexico has been scantily studied. the aim of the study was to determine the seroprevalence and correlates of e. histolytica antibodies in adults in rural areas in durango, mexico. | 2015 | 25883706 |
| assessments of immunomodulatory and inflammatory effects against induction of entamoeba histolytica (hm1 ims strain) crude extract antigen in complete freund's adjuvant induced rheumatoid arthritis female wistar rats. | today it is well known about mechanisms of cell communication, how the cells that mediate immune response and tissue injury accumulate in tissues but the aetiology of rheumatoid arthritis (ra) is still unknown. this study was to evaluate immunomodulatory effects of crude entamoeba histolytica (hm1 ims strain) antigen in complete freund's adjuvant female wistar rats by studying the alterations in humoral and cell mediated immune responses and also the inflammatory effects by evaluating the change ... | 2015 | 25881902 |
| antiprotozoal nitazoxanide derivatives: synthesis, bioassays and qsar study combined with docking for mechanistic insight. | in view of the serious health problems concerning infectious diseases in heavily populated areas, we followed the strategy of lead compound diversification to evaluate the near-by chemical space for new organic compounds. to this end, twenty derivatives of nitazoxanide (ntz) were synthesized and tested for activity against entamoeba histolytica parasites. to ensure drug-likeliness and activity relatedness of the new compounds, the synthetic work was assisted by a quantitative structure-activity ... | 2015 | 25872791 |
| roles and regulation of the mucus barrier in the gut. | the gastrointestinal tract is coated by a thick layer of mucus that forms the front line of innate host defense. mucus consists of high molecular weight glycoproteins called mucins that are synthesized and secreted by goblet cells and functions primarily to lubricate the epithelium and protect it from damage by noxious substances. recent studies have also suggested the involvement of goblet cells and mucins in complex immune functions such as antigen presentation and tolerance. under normal phys ... | 2015 | 25838985 |
| correction: crystal structure of calcium binding protein-5 from entamoeba histolytica and its involvement in initiation of phagocytosis of human erythrocytes. | 2015 | 25816121 | |
| pyrazolo[3,4-d]pyrimidines as novel inhibitors of o-acetyl-l-serine sulfhydrylase of entamoeba histolytica: an in silico study. | amoebiasis, a worldwide explosive epidemic, caused by the gastrointestinal anaerobic protozoan parasite entamoeba histolytica, infects the large intestine and, in advance stages, liver, kidney, brain and lung. metronidazole (mnz)-the first line medicament against amoebiasis-is potentially carcinogenic to humans and shows significant side-effects. pyrazolo[3,4-d]pyrimidine compounds have been reported to demonstrate antiamoebic activity. in silico molecular docking simulations on nine pyrazolo[3, ... | 2015 | 25799964 |
| homology modeling of mdr1 gene mdr1_enthi of e. histolytica & its molecular docking with anti-entamoeba histolytica agents. | to investigate the theoretical model of the three-dimensional structure of multidrug resistance protein 1(p-glycoprotein, expressed by mdr1 gene of e. histolytica) and its molecular docking with anti-amoebic agents. the theoretical model of multidrug resistance protein 1(p-glyoprotein, expressed by mdr1 gene of e. histolytica) was predicted by homology modeling on the structure of the 3g61. docking studies were performed to investigate the interaction of p-glycoprotein with antiamoebic gents. mr ... | 2015 | 25786505 |
| functional correlation of medicinal chemistry and entamoeba histolytica treatment: an emphasis on the past, present and future chemotherapy. | entamoeba being an internal parasite is the causative organism of many human and animal infections with lethal consequences. chemotherapy against entamoeba was well established by both synthetic and natural products. vigorous attainments were made by different researchers all over the world for discovering better chemical entities and the potent formulations. preexisting compounds were developed into non-cytotoxic and with an enhanced therapeutic compounds by usage of multiparametric optimizatio ... | 2015 | 25769970 |
| enteric pathogens of food sellers in rural gambia with incidental finding of myxobolus species (protozoa: myxozoa). | ongoing surveillance of enteric pathogens of public health significance among casual food sellers is undertaken in many resource-limited countries. we report the results of a survey in kiang west province, the gambia, and provide an exemplar methodology for such surveys in resource-limited laboratories. | 2015 | 25758855 |
| a rare case of multilocus brain abscess due to entamoeba histolytica infection in a child. | brain abscess due to entamoeba histolytica (e. histolytica) may pose a diagnostic problem or a therapeutic challenge, as evidenced by the paucity of papers reporting complete recovery after treatment. an 11-year-old girl presented with progressive drowsiness, diminished movements of the left upper limb, and swallowing problems. cranial mri showed multiple, contrast-dense masses with fluid content. she was started on meropenem. surgical drainage was performed. no bacterial or fungal growth was ob ... | 2015 | 25737180 |
| small gtpase rab21 mediates fibronectin induced actin reorganization in entamoeba histolytica: implications in pathogen invasion. | the protozoan parasite entamoeba histolytica causes a wide spectrum of intestinal infections. in severe cases, the trophozoites can breach the mucosal barrier, invade the intestinal epithelium and travel via the portal circulation to the liver, where they cause hepatic abscesses, which can prove fatal if left untreated. the host extra cellular matrix (ecm) plays a crucial role in amoebic invasion by triggering an array of cellular responses in the parasite, including induction of actin rich adhe ... | 2015 | 25730114 |
| new enzymatic pathways for the reduction of reactive oxygen species in entamoeba histolytica. | entamoeba histolytica, an intestinal parasite that is the causative agent of amoebiasis, is exposed to elevated amounts of highly toxic reactive oxygen and nitrogen species during tissue invasion. a flavodiiron protein and a rubrerythrin have been characterized in this human pathogen, although their physiological reductants have not been identified. | 2015 | 25725270 |
| a novel mitosomal β-barrel outer membrane protein in entamoeba. | entamoeba possesses a highly divergent mitochondrion-related organelle known as the mitosome. here, we report the discovery of a novel protein in entamoeba, which we name mitosomal β-barrel outer membrane protein of 30 kda (mbomp30). initially identified through in silico analysis, we experimentally confirmed that mbomp30 is indeed a β-barrel protein. circular dichroism analysis showed mbomp30 has a predominant β-sheet structure. localization to entamoeba histolytica mitosomes was observed throu ... | 2015 | 25711150 |
| prevalence of entamoeba histolytica -like cysts compared to e. histolytica antigens detected by elisa in the stools of 600 patients from three socioeconomic communities in the metropolitan city of lahore, pakistan. | amoebiasis, caused by entamoeba histolytica , has a worldwide distribution and is of public health significance in many developing countries. it has a fecal-oral transmission cycle and is most prevalent in developing countries in regions where substandard sanitary conditions exist due to poverty. little is known about the epidemiology of e. histolytica infection and its presence in different socioeconomic communities in developing countries. we undertook the present study in the city of lahore, ... | 2015 | 25189631 |
| [clinical case of the month. amoebic liver abscess contracted in india with diagnosis confirmed by pcr]. | amoebiasis is a disease of parasitic origin responsible for dysentery and extra-intestinal complications. the infection is by entamoeba histolytica, an amoeba whose geographical distribution is cosmopolitan, but that is more prevalent in tropical areas. only a few infections are symptomatic and some of them may cause extra-intestinal complications. hepatic amoebiasis is the most frequently observed. we report the case of a belgian woman who developed an amoebic liver abscess after returning from ... | 2015 | 24053104 |
| an amebic anti-inflammatory peptide down-regulates ex vivo il-1β expression in patients with rheumatoid arthritis. | the monocyte locomotion inhibitory factor (mlif) is a heat-stable pentapeptide produced by entamoeba histolytica in culture. this factor displays several anti-inflammatory properties (i.e., inhibition of locomotion and respiratory burst in monocytes, reduction of skin hypersensitivity and delay of mononuclear cells in human rebuck skin windows) with inhibition of adhesion molecules, chemokines, and other genes including interleukin-1β (il-1β). in animal models, it reduces carragenin-induced infl ... | 2015 | 22749729 |
| amoebic cervicitis mimicking posterior wall fibroid: a rare presentation. | amoebiasis of the uterine cervix is an extremely rare entity, and presentation as fibroid uterus has not been reported, to the best of our knowledge in our extensive search of the english literature. it can clinically simulate cervical malignancy by virtue of surface papillomatous and overall ulcerated and necrotic appearance. we present a case of amoebic infection of the cervix in a 45-year-old female which was suspected to be a posterior wall fibroid with degeneration until a histopathological ... | 2014 | 22743593 |
| leptin signaling protects the gut from entamoeba histolytica infection. | the role of the adipose-derived hormone leptin, and leptin receptors, in signaling satiety to the central nervous system and regulating energy balance is well recognized. but leptin also acts on peripheral tissues such as skeletal muscles, adipose tissues, pancreas, liver, intestine and the immune system. the existence of different splice variants of leptin receptor and the numerous intracellular signaling pathways triggered by leptin make this a truly versatile system. two recent studies explor ... | 2014 | 22356851 |
| degradation of the transcription factors nf-κb, stat3, and stat5 is involved in entamoeba histolytica-induced cell death in caco-2 colonic epithelial cells. | entamoeba histolytica is a tissue-invasive protozoan parasite causing dysentery in humans. during infection of colonic tissues, amoebic trophozoites are able to kill host cells via apoptosis or necrosis, both of which trigger il-8-mediated acute inflammatory responses. however, the signaling pathways involved in host cell death induced by e. histolytica have not yet been fully defined. in this study, we examined whether calpain plays a role in the cleavage of pro-survival transcription factors d ... | 2014 | 25352693 |
| expression, purification, and evaluation of recombinant leca as a candidate for an amebic colitis vaccine. | entamoeba histolytica, which causes amebic colitis and liver abscess, is considered a major enteric pathogen in residents and travelers to developing countries where the disease is endemic. interaction of this protozoan parasite with the intestine is mediated through the binding of the trophozoite stage to intestinal mucin and epithelium via a galactose and n-acetyl-d-galactosamine (gal/galnac) lectin comprised of a disulfide linked heavy (ca. 180 kda) and light chain (ca. 35 kda) and a noncoval ... | 2014 | 23827311 |
| the cell surface proteome of entamoeba histolytica. | surface molecules are of major importance for host-parasite interactions. during entamoeba histolytica infections, these interactions are predicted to be of prime importance for tissue invasion, induction of colitis and liver abscess formation. to date, however, little is known about the molecules involved in these processes, with only about 20 proteins or protein families found exposed on the e. histolytica surface. we have therefore analyzed the complete surface proteome of e. histolytica. usi ... | 2014 | 24136294 |
| discovery of entamoeba histolytica hexokinase 1 inhibitors through homology modeling and virtual screening. | entamoeba histolytica, the parasite which causes amebiasis is responsible for 110,000 deaths a year. entamoeba histolytica depends on glycolysis to obtain atp for cellular work. according to metabolic flux studies, hexokinase exerts the highest flux control of this metabolic pathway; therefore, it is an excellent target in the search of new antiamebic drugs. to this end, a tridimensional model of e. histolytica hexokinase 1 (ehhk1) was constructed and validated by homology modeling. after virtua ... | 2014 | 23534932 |
| entamoeba histolytica acetyl-coa synthetase: biomarker of acute amoebic liver abscess. | to characterize the entamoeba histolytica (e. histolytica) antigen(s) recognized by moribound amoebic liver abscess hamsters. | 2014 | 25182945 |
| gal-lectin-dependent contact activates the inflammasome by invasive entamoeba histolytica. | entamoeba histolytica (eh) is an extracellular protozoan parasite of the human colon, which occasionally breaches the intestinal barrier. eradicating ameba that invades is essential for host survival. a defining but uncharacterized feature of amebic invasion is direct contact between ameba and host cells. this event corresponds with a massive pro-inflammatory response. to date, pathogen recognition receptors (prrs) that are activated by contact with viable eh are unknown. here we show that the i ... | 2014 | 24253103 |
| deciphering molecular mechanisms of mrna metabolism in the deep-branching eukaryote entamoeba histolytica. | although extraordinary rapid advance has been made in the knowledge of mechanisms regulating messenger rna (mrna) metabolism in mammals and yeast, little information is known in deep-branching eukaryotes. the complete genome sequence of entamoeba histolytica, the protozoan parasite responsible for human amoebiasis, provided a lot of information for the identification and comparison of regulatory sequences and proteins potentially involved in mrna synthesis, processing, and degradation. here, we ... | 2014 | 24249245 |
| the ribosomal rna transcription unit of entamoeba invadens: accumulation of unprocessed pre-rrna and a long non coding rna during encystation. | the ribosomal rna genes in entamoeba spp. are located on extrachromosomal circular molecules. unlike model organisms where rrna transcription stops during growth stress, entamoeba histolytica continues transcription; but unprocessed pre-rrna accumulates during stress, along with a novel class of circular transcripts from the 5'-external transcribed spacer (ets). to determine the fate of rrna transcription during stage conversion between trophozoite to cyst we analyzed entamoeba invadens, a model ... | 2014 | 24200639 |
| rickettsiae, protozoa, and opisthokonta/metazoa. | rhizobiales (formerly named rickettsiales) cause in rare instances meningitis and meningovasculitis, respectively. in case of history of exposure, infection by rhizobiales needs to be considered since both diagnosis and therapy may be extremely difficult and pathogen-specific. the same applies to protozoa; in this chapter, babesia species, free-living amoebae and entamoeba histolytica infection, including severe meningitis and brain abscess, infection by trypanosoma species (south american and a ... | 2014 | 24365428 |
| evaluation of the effect of miltefosine on trichomonas vaginalis. | trichomonas vaginalis causes trichomoniasis in humans, a sexually transmitted disease commonly treated with metronidazole (mtz). mtz is known to cause undesirable side effects, and mtz-resistant parasites have been reported. thus, the development of an alternative treatment is desirable. miltefosine (mlt) is an alkylphosphocholine synthetic lipid analogue that displays antiparasitic activity against leishmania, trypanosoma cruzi, entamoeba histolytica, acanthamoeba spp., giardia lamblia, t. vagi ... | 2014 | 24363204 |
| clinical significance of high anti-entamoeba histolytica antibody titer in asymptomatic hiv-1-infected individuals. | anti-entamoeba histolytica antibody (anti- e. histolytica) is widely used in seroprevalence studies though its clinical significance has not been assessed previously. | 2014 | 24338349 |
| multilocus sequence typing system (mlst) reveals a significant association of entamoeba histolytica genetic patterns with disease outcome. | the relationship between parasite genotypes and outcome of amoebic infection is still a paradox and needed to be explored. proper identification and genetic characterization of entamoeba histolytica clinical isolates is an effective tool for exploring this relation. along with conventional polymorphic marker (chitinase), trna linked short tandem repeat (str) loci has been employed as multilocus genotyping tool due to its better resolution and evolutionary significance. some common as well as exc ... | 2014 | 24321887 |
| analysis of u3 snorna and small subunit processome components in the parasitic protist entamoeba histolytica. | in the early branching parasitic protist entamoeba histolytica, pre-rrna synthesis continues when cells are subjected to growth stress, but processing slows down and unprocessed pre-rrna accumulates. to gain insight into the regulatory mechanisms leading to accumulation, it is necessary to define the pre-rrna processing machinery in e. histolytica. we searched the e. histolytica genome sequence for homologs of the ssu processome, which contains the u3snorna, and 72 proteins in yeast. we could id ... | 2014 | 24631428 |
| proteomic identification of s-nitrosylated proteins in the parasite entamoeba histolytica by resin-assisted capture: insights into the regulation of the gal/galnac lectin by nitric oxide. | entamoeba histolytica is a gastrointestinal protozoan parasite that causes amebiasis, a disease which has a worldwide distribution with substantial morbidity and mortality. nitrosative stress, which is generated by innate immune cells, is one of the various environmental challenges that e. histolytica encounters during its life cycle. although the effects of nitric oxide (no) on the regulation of gene expression in this parasite have been previously investigated, our knowledge on s-nitrosylated ... | 2014 | 24626316 |
| post-travel screening of asymptomatic long-term travelers to the tropics for intestinal parasites using molecular diagnostics. | the incidence of asymptomatic travel-related parasitic infection is uncertain. previous studies did not distinguish new incident infections, from past infections. regardless of symptoms, we performed multiplex real-time polymerase chain reaction on pre- and post-travel stool samples of dutch long-term travelers to the (sub)tropics. serological screening for schistosoma spp. was only performed in travelers to sub-saharan africa. in total, 679 travelers were included in the study. the follow-up ra ... | 2014 | 24615130 |
| entamoeba histolytica: gene expression analysis of cells invading tissues. | entamoeba histolytica is a protozoan parasite that presents a risk to the health of millions of people worldwide. due to the existence of different clinical forms caused by the parasite and also different virulence levels presented by one strain, one would expect differences in the profile of gene transcripts between virulent and nonvirulent cultures. in this study we used the differential display to select gene segments related to invasiveness of amoeba. one brazilian strain of e. histolytica i ... | 2014 | 24605052 |
| laboratory diagnosis of amebiasis in a sample of students from southeastern brazil and a comparison of microscopy with enzyme-linked immunosorbent assay for screening of infections with entamoeba sp. | epidemiological studies on amebiasis have been reassessed since entamoeba histolytica and e. dispar were first recognized as distinct species. because the morphological similarity of these species renders microscopic diagnosis unreliable, additional tools are required to discriminate between entamoeba species. the objectives of our study were to compare microscopy with elisa kit (ivd®) results, to diagnose e. histolytica infection, and to determine the prevalence of amebiasis in a sample of stud ... | 2014 | 24603737 |
| the entamoeba histolytica dnmt2 homolog (ehmeth) confers resistance to nitrosative stress. | nitric oxide (no) has antimicrobial properties against many pathogens due to its reactivity as an s-nitrosylating agent. it inhibits many of the key enzymes that are involved in the metabolism and virulence of the parasite entamoeba histolytica through s-nitrosylation of essential cysteine residues. very little information is available on the mechanism of resistance to no by pathogens in general and by this parasite in particular. here, we report that exposure of the parasites to s-nitrosoglutat ... | 2014 | 24562908 |
| risk factors for intestinal parasitosis among antiretroviral-treated hiv/aids patients in ethiopia. | summary a cross-sectional survey was conducted to determine the risk factors associated with intestinal parasitosis in hiv/aids patients receiving antiretroviral therapy (art). socio-demographic information was collected and faecal samples were analysed from 384 randomly selected patients on art. data on cd4+ t-cell counts and world health organization clinical staging were obtained from the medical records at the hospital. the overall prevalence of intestinal parasitosis was 56% (95% confidence ... | 2014 | 24554001 |
| unusual multiple large abscesses of the liver: interest of the radiological features and the real-time pcr to distinguish between bacterial and amebic etiologies. | we report a rare case of amebiasis generating 19 large liver abscesses. such a quantity of abscesses is rare, especially when occurring in a young casual traveler without any immunodeficiency disorders. a possible co-infection was excluded. by contrast, the amebic etiology was confirmed by means of serology and real-time pcr. | 2014 | 24548161 |
| expression of ehrad54, ehrad51, and ehblm proteins during dna repair by homologous recombination in entamoeba histolytica. | entamoeba histolytica, the protozoan responsible for human amoebiasis, exhibits a great genome plasticity that is probably related to homologous recombination events. it contains the rad52 epistasis group genes, including ehrad51 and ehrad54, and the ehblm gene, which are key homologous recombination factors in other organisms. ehrad51 and ehrad54 genes are differentially transcribed in trophozoites when dna double-strand breaks are induced by ultraviolet-c irradiation. moreover, the ehrad51 rec ... | 2014 | 24534563 |
| synthesis of 4'-(2-ferrocenyl)-2,2':6'2''-terpyridine: characterization and antiprotozoal activity of mn(ii), co(ii), ni(ii), cu(ii) and zn(ii) complexes. | a terpyridine ligand fctpy was reacted with divalent metals (cu, co, mn, ni and zn), yielding five complexes of general formula [metal(fctpy)2][pf6]2. the structure of fctpy was determined by single crystal x-ray diffraction studies. the complexes characterized using various spectroscopic techniques suggested an octahedral geometry around the central metal ion. these complexes were screened for their antiamoebic, trypanocidal and antimalarial activities. it was found that, complexes 2 and 3 show ... | 2014 | 24534536 |
| design, synthesis and biological evaluation of 3-[4-(7-chloro-quinolin-4-yl)-piperazin-1-yl]-propionic acid hydrazones as antiprotozoal agents. | n-acylhydrazones derived from 7-chloro-4-piperazin-1-yl-quinoline were synthesized and biologically evaluated for blood-stage of plasmodium falciparum and entamoeba histolytica trophozoites. n-acylhydrazone f12 was found to inhibit the p. falciparum growth as well as its life cycle with good selectivity, which was achieved by inhibiting hematin formation. compound f24 showed better ic50 value than the amoebicidal drug metronidazole. | 2014 | 24530492 |
| the future for vaccine development against entamoeba histolytica. | entamoeba histolytica is the causative agent of amebiasis, one of the top three parasitic causes of mortality worldwide. in the majority of infected individuals, e. histolytica asymptomatically colonizes the large intestine, while in others, the parasite breaches the mucosal epithelial barrier to cause amebic colitis and can disseminate to soft organs to cause abscesses. vaccinations using native and recombinant forms of the parasite gal-lectin have been successful in protecting animals against ... | 2014 | 24504133 |
| entamoeba histolytica-secreted cysteine proteases induce il-8 production in human mast cells via a par2-independent mechanism. | entamoeba histolytica is an extracellular tissue parasite causing colitis and occasional liver abscess in humans. e. histolytica-derived secretory products (sps) contain large amounts of cysteine proteases (cps), one of the important amoebic virulence factors. although tissue-residing mast cells play an important role in the mucosal inflammatory response to this pathogen, it is not known whether the sps induce mast cell activation. in this study, when human mast cells (hmc-1 cells) were stimulat ... | 2014 | 24502918 |
| structure, absolute configuration, and antidiarrheal activity of a thymol derivative from ageratina cylindrica. | the leaves of ageratina cylindrica afforded a thymol derivative that was characterized by physical and spectroscopical methods as (8s)-8,9-epoxy-6-hydroxy-l0-benzoyloxy-7-oxothymol isobutyrate (1). the absolute configuration of 1 was established as 8s by vibrational circular dichroism spectroscopy in combination with density functional theory calculations and by evaluation of the flack and hooft x-ray parameters. compound 1 showed weak antiprotozoal activity against entamoeba histolytica and gia ... | 2014 | 24502360 |
| association between allelic variation due to short tandem repeats in trna gene of entamoeba histolytica and clinical phenotypes of amoebiasis. | genotypes of entamoeba histolytica (e. histolytica) may contribute clinical phenotypes of amoebiasis such as amoebic liver abscess (ala), dysentery and asymptomatic cyst passers state. hence, we evaluated allelic variation due to short tandem repeats (strs) in trna gene of e. histolytica and clinical phenotypes of amoebiasis. asymptomatic cyst passers (n=24), patients with dysentery (n=56) and ala (n=107) were included. extracted dna from stool (dysentery, asymptomatic cyst passers) and liver as ... | 2014 | 24495629 |
| a novel c-terminal homologue of aha1 co-chaperone binds to heat shock protein 90 and stimulates its atpase activity in entamoeba histolytica. | cytosolic heat shock protein 90 (hsp90) has been shown to be essential for many infectious pathogens and is considered a potential target for drug development. in this study, we have carried out biochemical characterization of hsp90 from a poorly studied protozoan parasite of clinical importance, entamoeba histolytica. we have shown that entamoeba hsp90 can bind to both atp and its pharmacological inhibitor, 17-aag (17-allylamino-17-demethoxygeldanamycin), with kd values of 365.2 and 10.77 μm, r ... | 2014 | 24486610 |
| insights into the gtp/gdp cycle of rabx3, a novel gtpase from entamoeba histolytica with tandem g-domains. | members of the small gtpase ras superfamily regulate a host of systems through their ability to catalyze the gtp/gdp cycle. all family members reported thus far possess a single gtpase domain with a p-loop containing a nucleoside triphosphate hydrolase fold. here for the first time we report a novel member from entamoeba histolytica, ehrabx3, which harbors two gtpase domains in tandem and exhibits unique biochemical properties. a combination of biochemical and microcalorimetric studies revealed ... | 2014 | 24471929 |
| stress granule formation in entamoeba histolytica: cross-talk between ehmlbp, ehrle3 reverse transcriptase and polyubiquitinated proteins. | the entamoeba histolytica-methylated line-binding protein (ehmlbp) binds to methylated repetitive dna and is a positive regulator of a reverse transcriptase of a long interspersed nucleotide element (line). this protein protects trophozoites against heat shock by reducing protein aggregation. the presence of ehmlbp and polyubiquitinated proteins in heat shock-induced protein aggregates raised the question whether these proteins interact. this assumption was confirmed by co-immunoprecipitation ex ... | 2014 | 24471581 |
| synthesis of 3-tetrazolylmethyl-4h-chromen-4-ones via ugi-azide and biological evaluation against entamoeba histolytica, giardia lamblia and trichomona vaginalis. | the synthesis of novel 3-tetrazolylmethyl-4h-chromen-4-ones via an ugi-azide multicomponent reaction and their biological evaluation against entamoeba histolytica, giardia lamblia and trichomona vaginalis are described. reported yields are moderate to good and biological results show that these compounds could be considered as candidates to anti-parasitic drugs, especially against g. lamblia. | 2014 | 24468633 |
| a genomewide overexpression screen identifies genes involved in the phosphatidylinositol 3-kinase pathway in the human protozoan parasite entamoeba histolytica. | entamoeba histolytica is a protozoan parasite that causes amoebic dysentery and liver abscess. e. histolytica relies on motility, phagocytosis, host cell adhesion, and proteolysis of extracellular matrix for virulence. in eukaryotic cells, these processes are mediated in part by phosphatidylinositol 3-kinase (pi3k) signaling. thus, pi3k may be critical for virulence. we utilized a functional genomics approach to identify genes whose products may operate in the pi3k pathway in e. histolytica. we ... | 2014 | 24442890 |
| nano-yeast-scfv probes on screen-printed gold electrodes for detection of entamoeba histolytica antigens in a biological matrix. | the time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. herein, we present low-cost affinity reagents, nano-yeast-scfv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free elec ... | 2014 | 24434498 |
| bioassay-guided fractionation of extracts from codiaeum variegatum against entamoeba histolytica discovers compounds that modify expression of ceramide biosynthesis related genes. | leaves of codiaeum variegatum ("garden croton") are used against bloody diarrhoea by local populations in cameroon. this study aims to search for the active components from c. variegatum against entamoeba histolytica, and thereby initiate the study of their mechanism of action. a bioassay-guided screening of the aqueous extracts from c. variegatum leaves and various fractions was carried out against trophozoites of e. histolytica axenic culture. we found that the anti-amoebic activity of extract ... | 2014 | 24416462 |
| prevalence of intestinal parasites among the population of the gaza strip, palestine. | intestinal parasitic diseases in gaza strip are a significant health problem. the main aim of this study is to determine the prevalence of parasitic infection among patients in the five governorates of the gaza strip. a cross-sectional parasitological survey was conducted on 600 patients. stool specimens were examined microscopically for the presence of parasite eggs or larvae/cysts per wet amount by the saline iodine and formol-ether concentration method. of 600 subjects examined, 245 (40.8%) w ... | 2014 | 25706427 |
| antiprotozoal activity against entamoeba histolytica of plants used in northeast mexican traditional medicine. bioactive compounds from lippia graveolens and ruta chalepensis. | amoebiasis caused by entamoeba histolytica is associated with high morbidity and mortality is becoming a major public health problem worldwide, especially in developing countries. because of the side-effects and the resistance that pathogenic protozoa build against the standard antiparasitic drugs, e.g., metronidazole, much recent attention has been paid to plants used in traditional medicine around the world in order to find new antiprotozoal agents. we collected 32 plants used in northeast mex ... | 2014 | 25517343 |
| leptin receptor mutation results in defective neutrophil recruitment to the colon during entamoeba histolytica infection. | amebiasis is an enteric infection caused by entamoeba histolytica, with symptoms ranging in severity from asymptomatic colonization to dysentery. humans with the q223r leptin receptor mutation have increased susceptibility to amebiasis, but the mechanism has been unclear. using a mouse model expressing the mutation, we tested the impact of the q223r mutation on the innate immune response to e. histolytica infection. the 223r mutation resulted in delayed clearance of amebae from the cecum, as had ... | 2014 | 25516614 |
| evolutionary genomics and population structure of entamoeba histolytica. | amoebiasis caused by the gastrointestinal parasite entamoeba histolytica has diverse disease outcomes. study of genome and evolution of this fascinating parasite will help us to understand the basis of its virulence and explain why, when and how it causes diseases. in this review, we have summarized current knowledge regarding evolutionary genomics of e. histolytica and discussed their association with parasite phenotypes and its differential pathogenic behavior. how genetic diversity reveals pa ... | 2014 | 25505504 |
| crystal structure of calcium binding protein-5 from entamoeba histolytica and its involvement in initiation of phagocytosis of human erythrocytes. | entamoeba histolytica is the etiological agent of human amoebic colitis and liver abscess, and causes a high level of morbidity and mortality worldwide, particularly in developing countries. there are a number of studies that have shown a crucial role for ca2+ and its binding protein in amoebic biology. ehcabp5 is one of the ef hand calcium-binding proteins of e. histolytica. we have determined the crystal structure of ehcabp5 at 1.9 å resolution in the ca2+-bound state, which shows an unconvent ... | 2014 | 25502654 |
| data set for the proteomics analysis of the endomembrane system from the unicellular entamoeba histolytica. | entamoeba histolytica is the protozoan parasite agent of amebiasis, an infectious disease of the human intestine and liver. this parasite contact and kills human cells by an active process involving pathogenic factors. cellular traffic and secretion activities are poorly characterized in e. histolytica. in this work, we took advantage of a wide proteomic analysis to search for principal components of the endomembrane system in e. histolytica. a total of 5683 peptides matching with 1531 proteins ... | 2014 | 26217682 |
| therapy of liver abscesses. | liver abscess (la) is an uncommon but potentially life-threatening disease with significant morbidity and mortality. | 2014 | 26287275 |